Sampling equipment that stem cell was used
Technical Field
The utility model relates to a stem cell preparation technical field specifically is a sampling equipment that stem cell used.
Background
Stem cells are a kind of multipotential cells with self-replicating ability, which can be differentiated into a plurality of functional cells under certain conditions, so that the therapy of treating patients by using the differentiation ability of stem cells is widely applied in clinical medicine, the existing human stem cells for stem cell therapy are generally sampled from adipose cell tissues of human body, after being extracted from subcutaneous adipose layers of human body, the adipose stem cells can be obtained after a series of treatments such as centrifugation and shaking, in the existing sampling operation of stem cells in adipose cells, the centrifugation and shaking of adipose tissues in vitro are generally performed by means of a plurality of devices in cooperation, which need to be operated for a plurality of times, thus increasing the difficulty of sampling stem cells, in comparison with the design provided by the application No. 201920907963.8, this design allows the stem cell tube to be centrifuged and shaken, but when stem cells are precipitated, not only the stem cell tube needs to be shaken, but also the stem cell tube often needs to be turned upside down, and this design cannot be turned upside down.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, provide a sampling equipment that stem cell was used, at first can carry out the centrifugation to stem cell's sampling test tube, still can reverse stem cell's sampling test tube, guarantee that stem cell can separate out fast completely to this improves sample efficiency, and the stability of the sampling test tube of guaranteeing stem cell when centrifugation and reversal, can effectively solve the problem in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: a sampling device for stem cells comprises an outer case and a first motor;
an outer box body: an upper inlet hole is formed in the upper side of the inner box body, a second motor is fixed on the lower side of the inner box body, an inner box body is fixed at the output end of the second motor, a lower inlet hole is formed in the upper side of the inner box body, the lower inlet hole and the upper inlet hole are arranged in a vertically corresponding mode, a vertical plate is fixed in the inner box body, an electromagnet is fixed at the upper end of the right side of the vertical plate, the inner box body is driven to rotate through the second motor, a rotating frame in the inner box body can be indirectly rotated, and then a stem cell test tube placed in the rotating frame is centrifuged;
a first motor: the device is fixed on the left side inside the inner box body, a transmission rod is fixed at the output end of the inner box body, a rotating frame is fixed at the right end of the transmission rod in a rotating mode, the rotating frame and the lower inlet hole are arranged in a vertically corresponding mode, a supporting rod movably penetrates through the upper end of the left side of the rotating frame, an iron block is fixed at the left end of the supporting rod, the iron block and an electromagnet are arranged in a left-right corresponding mode, a second spring is fixed on the right side of the iron block, the other end of the second spring is fixed on the left side of the rotating frame, the supporting rod is located in the second spring, the rotating frame is driven to rotate through a first motor, the stem cell test tube is turned upside down, the stem cell test tube is supported through the supporting rod, stability of the stem cell test tube during turning upside down and centrifugation is guaranteed, the iron block is attracted leftwards through the electromagnet;
wherein: the electric box further comprises a single chip microcomputer which is fixed on the upper side of the outer box body, the input end of the single chip microcomputer is electrically connected with the output end of an external power supply, and the output end of the single chip microcomputer is electrically connected with the input ends of the first motor, the second motor and the electromagnet.
Further, still including supporting seat and shock attenuation flexible glue board, the downside at the outer box is fixed to the upside of supporting seat, the downside of supporting seat is fixed with the shock attenuation flexible glue board, improves this device through the supporting seat and lays the stability on the workstation, reduces the vibrations of this device when the centrifugation through the shock attenuation flexible glue board.
Further, still including outer push pedal and first spring, the inside bottom side at the revolving rack is fixed to the one end of first spring, the downside at outer push pedal is fixed to the other end of first spring, outer push pedal activity interlude is in the revolving rack, through the outer push pedal of first spring upwards promotion, and outer push pedal upwards promotes the test tube, makes things convenient for taking out of test tube.
Furthermore, the motor also comprises a supporting transverse plate which is fixed in the outer box body, the output end of the second motor rotates to penetrate through the supporting transverse plate, the supporting transverse plate can assist the second motor to support the inner box body, and the service life of the second motor is prolonged.
Further, still including the balancing weight, fix on the right side of interior box, when box rotated in making the second motor drive through the balancing weight, the center of rotation of guaranteeing interior box was located the output of second motor directly over, improved the life of second motor.
Compared with the prior art, the beneficial effects of the utility model are that: the sampling device for stem cells has the following advantages:
1. drive interior box through the second motor and rotate, can indirectly make the revolving rack that is located the interior box rotate, and then will put into the stem cell test tube of revolving rack and carry out the centrifugation, drive the revolving rack through first motor and rotate, and then make the stem cell test tube overturn the upset, support the stem cell test tube through supporting the pole, guarantee the stability of stem cell test tube when overturning upset and centrifugation, attract the iron plate left through the electro-magnet, and then make to support the pole and move to the left, make things convenient for the stem cell test tube to put into in the revolving rack.
2. Improve the stability on this device lays the workstation through the supporting seat, reduce the vibrations of this device when the centrifugation through the shock attenuation flexible glue board, upwards promote the push-out board through first spring, the push-out board upwards promotes the test tube, make things convenient for taking out of test tube, can assist the interior box of second motor support through supporting the diaphragm, improve the life of second motor, when box rotates in making the second motor drive through the balancing weight, the rotation center of box is located directly over the output of second motor in guaranteeing, improve the life of second motor.
3. This sampling equipment that stem cell was used can at first carry out the centrifugation to stem cell's sampling test tube, still can reverse stem cell's sampling test tube, guarantees that stem cell can separate out fast totally to this improves sample efficiency, and guarantees stem cell's sampling test tube's stability when centrifugation and reversal.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top view of the present invention;
fig. 3 is a schematic view of the cross-sectional structure at a-a of the present invention.
In the figure: the device comprises an inner box body 1, a single chip microcomputer 2, an outer box body 3, a supporting seat 4, a damping soft rubber plate 5, a rotating frame 6, a supporting rod 7, an outer pushing plate 8, a vertical plate 9, a first motor 10, a transmission rod 11, a supporting transverse plate 12, a second motor 13, a balancing weight 14, a first spring 15, a lower inlet hole 16, a second spring 17, an iron block 18, an upper inlet hole 19 and an electromagnet 20.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a sampling device for stem cells comprises an outer case 3 and a first motor 10;
outer box 3: the upside is provided with an upper inlet hole 19, the inner downside is fixed with a second motor 13, the output end of the second motor 13 is fixed with an inner box body 1, the upside of the inner box body 1 is provided with a lower inlet hole 16, the lower inlet hole 16 and the upper inlet hole 19 are arranged corresponding to each other up and down, a vertical plate 9 is fixed in the inner box body 1, the upper end of the right side of the vertical plate 9 is fixed with an electromagnet 20, the inner box body 1 is driven to rotate by the second motor 13, a rotating frame 6 positioned in the inner box body 1 can be indirectly rotated, then a stem cell test tube placed in the rotating frame 6 is centrifuged, the device also comprises a supporting seat 4 and a damping soft rubber plate 5, the upside of the supporting seat 4 is fixed at the downside of the outer box body 3, the lower side of the supporting seat 4 is fixed with the damping soft rubber plate 5, the stability of the device placed on a workbench is improved by the supporting seat 4, the vibration of the device during, the output end of the second motor 13 penetrates through the supporting transverse plate 12 in a rotating mode, the second motor 13 can be assisted to support the inner box body 1 through the supporting transverse plate 12, the service life of the second motor 13 is prolonged, the counterweight block 14 is further included and is fixed on the right side of the inner box body 1, when the inner box body 1 is driven to rotate by the second motor 13 through the counterweight block 14, the rotating center of the inner box body 1 is guaranteed to be located right above the output end of the second motor 13, and the service life of the second motor 13 is prolonged;
first motor 10: the device is fixed on the left side in the inner box body 1, the output end of the device is fixed with a transmission rod 11, the right end of the transmission rod 11 is rotatably penetrated through a vertical plate 9 to be fixed with a rotating frame 6, the rotating frame 6 is vertically and correspondingly arranged with a lower inlet hole 16, the upper end of the left side of the rotating frame 6 is movably penetrated through a supporting rod 7, the left end of the supporting rod 7 is fixed with an iron block 18, the iron block 18 is horizontally and correspondingly arranged with an electromagnet 20, the right side of the iron block 18 is fixed with a second spring 17, the other end of the second spring 17 is fixed on the left side of the rotating frame 6, the supporting rod 7 is positioned in the second spring 17, the rotating frame 6 is driven by a first motor 10 to rotate, so that a stem cell test tube is reversely turned, the supporting rod 7 is supported against the stem cell test tube, the stability of the stem cell test tube during reverse turning and centrifugation is ensured, the electromagnet 20 attracts the iron block 18 leftwards, so that the supporting rod 7 moves leftwards, one end of a first spring 15 is fixed on the bottom side of the interior of the rotating frame 6, the other end of the first spring 15 is fixed on the lower side of the outer push plate 8, the outer push plate 8 is movably inserted into the rotating frame 6, the outer push plate 8 is pushed upwards through the first spring 15, and the outer push plate 8 pushes the test tube upwards, so that the test tube can be conveniently taken out;
wherein: the electric box further comprises a single chip microcomputer 2 fixed on the upper side of the outer box body 3, the input end of the single chip microcomputer is electrically connected with the output end of an external power supply, and the output end of the single chip microcomputer is electrically connected with the input ends of the first motor 10, the second motor 13 and the electromagnet 20.
When in use: when the single chip microcomputer 2 is opened, the electromagnet 20 starts to work, the electromagnet 20 attracts the iron block 18 leftwards, the iron block 18 pulls the abutting rod 7 leftwards, the second spring 17 is stretched, the stem cell test tube is fed into the rotating frame 6 from the upper inlet hole 19 and the lower inlet hole 16, the stem cell test tube presses the outer push plate 8 downwards, the first spring 15 is compressed, the single chip microcomputer 2 controls the electromagnet 20 to stop working, the second spring 17 contracts, the second spring 17 pulls the iron block 18 rightwards, the abutting rod 7 moves rightwards, the abutting rod 7 abuts against the upper end of the test tube, when centrifugation is needed, the single chip microcomputer 2 controls the second motor 13 to work, the second motor 13 drives the inner box body 1 to rotate, parts in the inner box body 1 all rotate, the rotating frame 6 drives the test tube to rotate for centrifugation, when inversion is needed, the single chip microcomputer 2 controls the first motor 10 to work, the first motor 10 drives the first motor 11 to rotate, the transmission rod 11 drives the rotating frame 6 to, the turret 6 brings the test tubes upside down.
It should be noted that the first electric motor 10 and the second electric motor 13 disclosed in the present embodiment may be implemented by OT-20GB type reduction motors manufactured by wayoda motors, inc, and the electromagnet 20 may be implemented by a small-sized DYSD type electromagnet manufactured by beijing engli mechanical equipment, inc. The single chip microcomputer 2 controls the first motor 10, the second motor 13 and the electromagnet 20 to work by adopting a method commonly used in the prior art.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.