CN115970914B - Centrifugal equipment and method for detecting cell proteins by PCR Array technology - Google Patents
Centrifugal equipment and method for detecting cell proteins by PCR Array technology Download PDFInfo
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- CN115970914B CN115970914B CN202310181179.4A CN202310181179A CN115970914B CN 115970914 B CN115970914 B CN 115970914B CN 202310181179 A CN202310181179 A CN 202310181179A CN 115970914 B CN115970914 B CN 115970914B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses centrifugal equipment and a method for detecting cell proteins by using a PCR Array technology, and belongs to the technical field of centrifugal equipment. The utility model provides a centrifugal equipment is used in cell protein detection to PCR Array technique, including box and hinged joint at the case lid at box top, fixed plate has been set firmly in the box, fixed plate rotation is connected with the rotor plate, be provided with the standing groove that is circumference evenly distributed on the rotor plate, be provided with the rack in the standing groove, placed the test tube on the rack, fixedly connected with actuating lever on the rotor plate, be provided with the actuating device who is used for driving actuating lever pivoted in the box, the actuating device outside is provided with the shock absorbing subassembly, still be provided with on the rack with shock absorbing subassembly matched with self-positioning subassembly; the invention effectively avoids the condition that the test tube is damaged due to overlarge vibration force, ensures the use stability of the test tube, can rapidly realize the putting in and taking out of the test tube at the test tube position, and greatly improves the centrifugal efficiency and the centrifugal effect.
Description
Technical Field
The invention relates to the technical field of centrifugal equipment, in particular to centrifugal equipment and a method for detecting cell proteins by using a PCR Array technology.
Background
Centrifugation is one of the most common methods for separating proteins, enzymes, nucleic acids and cellular sub-components, and is also a method for separating, purifying or clarifying in biochemical laboratories, and the centrifugation technology uses a strong centrifugal force generated when an object rotates at a high speed to enable suspended particles placed in a rotating body to settle or float, so that some particles achieve the purpose of concentrating or separating other particles, and the suspended particles often refer to cells, cellular organelles, viruses, biological macromolecules and the like which are made into a suspended state.
The existing centrifugal machine for cells can generate vibration and noise due to high-speed rotation of a motor, and a test tube is possibly damaged due to overlarge vibration force in the centrifugal process, so that the test tube is required to be manually fixed and limited, the test tube is prevented from flying out in the centrifugal process, the operation is complicated when the test tube is taken and placed, and the taking and placing efficiency of the test tube is affected; meanwhile, the existing centrifugal machine is provided with the placing groove for placing the test tube on the workbench, the placing groove is deeper, the outside leakage size of the test tube relative to the placing groove is small, and the placing groove is generally inclined, so that the lowering speed and the taking-out time of the test tube by workers are influenced, and the centrifugal efficiency is further influenced.
Disclosure of Invention
The invention aims to solve the problems in the prior art, and provides centrifugal equipment and a method for detecting cell proteins by using a PCR Array technology.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a PCR Array technique is centrifugal equipment for cell protein detects, includes box and hinged joint at the case lid at the box top, fixed plate has been set firmly in the box, the fixed plate internal rotation is connected with the rotor plate, be provided with the standing groove that is circumference evenly distributed on the rotor plate, be provided with the rack in the standing groove, the test tube has been placed on the rack, fixedly connected with actuating lever on the rotor plate, be provided with in the box and be used for driving actuating lever pivoted actuating device, the actuating device outside is provided with shock absorbing assembly, still be provided with on the rack with shock absorbing assembly matched with self-positioning assembly.
Preferably, the vibration absorbing assembly comprises a shell fixedly arranged in the box body and coated on the outer side of the driving device, a rotating pipe is rotationally connected to the inner wall of the shell, a storage cavity is formed between the shell and the rotating pipe, liquid is filled in the storage cavity, the rotating pipe is fixedly connected with the driving rod, a plurality of connecting plates are arranged on the outer side of the rotating pipe, a first pneumatic telescopic rod is connected to the connecting plates, and one end, far away from the connecting plates, of the first pneumatic telescopic rod is connected with a stress plate.
Preferably, the self-positioning assembly comprises an ear plate fixedly arranged on the placement frame, a movable groove is formed in the ear plate, a second pneumatic telescopic rod is arranged on the inner wall of the movable groove, one end, far away from the inner wall of the movable groove, of the second pneumatic telescopic rod is connected with a limiting clamping block, and an extrusion inclined plane is arranged on the limiting clamping block.
Preferably, the first pneumatic telescopic rod and the second pneumatic telescopic rod have the same structure and comprise sleeves, the two sleeves are respectively connected with the connecting plate and the inner wall of the movable groove, a piston is connected in each sleeve in a sliding manner, a second elastic element is arranged between the piston and the inner wall of the sleeve, and one side of the piston, which is away from the second elastic element, is connected with the movable rod.
Preferably, the self-positioning assembly further comprises an air guide groove arranged in the connecting plate, the driving rod and the rotating plate, one end of the air guide groove is connected with the first pneumatic telescopic rod, and the other end of the air guide groove is connected with the second pneumatic telescopic rod through a hose.
Preferably, the rack comprises an upper pipe body and a lower pipe body which are connected in a sliding mode, an accommodating groove used for sliding the upper pipe body is formed in the lower pipe body, and a first elastic element is arranged between the inner wall of the accommodating groove and the bottom of the upper pipe body.
Preferably, the outer side of the upper pipe body and the outer side of the lower pipe body are respectively connected with a first ring body and a second ring body in a rotating mode through a pin shaft, the first ring body is connected in a placing groove in a rotating mode, a swinging rod is movably connected to the second ring body, one end, far away from the second ring body, of the swinging rod is movably connected with a moving ring, the moving ring is sleeved on the outer side of a driving rod, a third elastic element is further sleeved on the outer side of the driving rod, two ends of the third elastic element are respectively connected with the moving ring and a shell, a connecting rod is connected to the outer side of the moving ring, a sliding block is fixedly connected to the connecting rod, a sliding groove used for sliding of the sliding block is formed in the box body, and a pull rope is connected between the sliding block and the box cover.
Preferably, the inner wall of the lower pipe body is provided with a rubber pad, a bottom cavity and a side cavity are arranged in the rubber pad, and a communication groove is arranged between the bottom cavity and the side cavity.
Preferably, the fixed bevel gear is fixedly connected to the fixed plate, and the driven bevel gear meshed with the fixed bevel gear is arranged on the placing frame.
The centrifugal method for detecting the cell protein by the PCR Array technology comprises the centrifugal equipment for detecting the cell protein by the PCR Array technology, and further comprises the following steps:
s1: when the device is used, the box cover is opened, the box cover is turned over relative to the box body, a pulling force is generated on the pulling rope when the box cover is turned over, the pulling rope drives the sliding block to move upwards in the sliding groove, the sliding block drives the movable ring to move upwards outside the driving rod through the connecting rod, the third elastic element is stretched, the movable ring pulls the second ring body through the swinging rod in the upward moving process, the second ring body drives the lower pipe body to move upwards relative to the upper pipe body, the first elastic element is compressed, the second ring body drives the lower pipe body to rotate by taking the connecting pin shaft of the first ring body and the placing groove as the circle center, the placing rack and the placing groove are in a vertical state, and then the test tubes are respectively arranged in different placing racks, and can be rapidly placed into the placing rack due to the fact that the placing rack is shallower and is in a straight line with the placing groove;
s2: then the box cover is turned over, so that the box cover is closed on the top of the box body again, the pull rope is not pulled by the box cover, the movable ring is reset and moves downwards under the pulling of the elastic force of the third elastic element, the movable ring drives the second ring body to move through the swinging rod, the second ring body drives the lower tube body to deflect relative to the placing groove, and the lower tube body slides relative to the upper tube body, so that the placing frame can accommodate a test tube with a larger area, the area of the test tube leaking out of the placing frame is reduced, and the protection and fixing effects on the test tube are improved;
s3: the driven bevel gear on the outer side of the rack after deflection is meshed with the fixed bevel gear on the fixed plate, and when the driving rod drives the rack to rotate through the rotating plate, the driven bevel gear on the rack rotates after being meshed with the fixed bevel gear;
s4: then controlling the driving equipment to operate, so that the output end of the driving equipment drives the driving rod to rotate, and the driving rod drives the placing rack at the placing groove to rotate through the rotating plate, so that the cells in the test tube are subjected to centrifugal operation;
s5: when the driving rod rotates, the driving rod drives the connecting plate to rotate in the shell through the rotating pipe, vibration is buffered and absorbed by utilizing the damping characteristic of liquid, noise and vibration are reduced when the driving rod rotates and operates, the risk of damage to the test tube caused by overlarge vibration force in the centrifuging process of the test tube is reduced, the connecting plate drives the stress plate to rotate in the liquid through the first pneumatic telescopic rod, the stress plate is stressed to extrude the first pneumatic telescopic rod, air in the first pneumatic telescopic rod enters the second pneumatic telescopic rod through the air guide groove and the hose, and the second pneumatic telescopic rod stretches and pushes the limiting clamping block to move in the movable groove, so that the limiting clamping block pushes the top of the test tube in the placing groove to position the top of the test tube;
s6: in the process that the limiting clamping block pushes the test tube, the test tube presses the bottom cavity of the rubber pad in the lower tube body, air in the bottom cavity enters the side cavity through the communicating groove, the side cavity expands to enable the rubber pad to press the side wall of the test tube, and the test tube in centrifugation is fixed efficiently.
Compared with the prior art, the invention provides the centrifugal equipment and the method for detecting the cell protein by the PCR Array technology, which have the following beneficial effects:
1. according to the centrifugal equipment and the method for detecting the cell protein by the PCR Array technology, when the driving rod rotates, the driving rod drives the connecting plate to rotate in the shell through the rotating pipe, vibration is buffered and absorbed by utilizing the damping characteristic of liquid, so that noise is reduced and vibration is reduced when the driving rod rotates and operates, the risk that a test tube is damaged due to overlarge vibration force in the centrifugal process is reduced, the connecting plate drives the stress plate to rotate in the liquid through the first pneumatic telescopic rod, the stress plate is stressed to extrude the first pneumatic telescopic rod, air in the first pneumatic telescopic rod enters the second pneumatic telescopic rod through the air guide groove and the hose, and the second pneumatic telescopic rod stretches and pushes the limiting clamping block to move in the movable groove, so that the limiting clamping block pushes the top of the test tube in the placing groove, the top of the test tube is positioned, the situation that the test tube is damaged due to overlarge vibration force is effectively avoided, and the stability of the use of the test tube is ensured.
2. According to the centrifugal equipment and the method for detecting the cell protein by the PCR Array technology, a pull rope is generated by turning over a box cover, the pull rope drives a sliding block to move upwards in a sliding groove, the sliding block drives a moving ring to move upwards outside a driving rod through a connecting rod, a third elastic element is stretched, the moving ring pulls a second ring body through a swinging rod in the upward moving process, a lower pipe body is driven by the second ring body to move upwards relative to an upper pipe body, a first elastic element is compressed, the second ring body drives the lower pipe body to rotate by taking a connecting pin shaft of the first ring body and a placing groove as a circle center, a placing rack and the placing groove are in a vertical state, and then test tubes are respectively arranged in different placing racks.
3. According to the centrifugal equipment and the method for detecting the cell protein by the PCR Array technology, the driven bevel gear on the outer side of the rack after deflection is meshed with the fixed bevel gear on the fixed plate, and when the drive rod drives the rack to rotate through the rotating plate, the driven bevel gear on the rack rotates after being meshed with the fixed bevel gear, so that the centrifugal effect on the test tube is further improved.
4. According to the centrifugal equipment and the method for detecting the cell protein by the PCR Array technology, when the test tube is pushed by the limiting clamping block, the test tube presses the bottom cavity of the rubber pad in the lower tube body, air in the bottom cavity enters the side cavity through the communicating groove, the side cavity expands to press the rubber pad to the side wall of the test tube, the test tube in centrifugation is efficiently fixed, and the use stability of the test tube is guaranteed.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of a cross-sectional structure of a case according to the present invention;
FIG. 3 is a partially enlarged schematic illustration of the structure of portion A of FIG. 2 in accordance with the present invention;
FIG. 4 is a partially enlarged schematic illustration of the structure of portion B of FIG. 2 in accordance with the present invention;
FIG. 5 is a schematic diagram of a cross-sectional structure of a case according to the present invention;
FIG. 6 is a schematic view of a rack according to the present invention;
FIG. 7 is a schematic view of the outer structure of the moving ring of the present invention;
FIG. 8 is a schematic cross-sectional view of a rack according to the present invention;
FIG. 9 is a schematic cross-sectional view of a lower pipe body according to the present invention;
FIG. 10 is a schematic cross-sectional view of an ear plate of the present invention;
fig. 11 is a schematic cross-sectional view of a first pneumatic telescopic rod according to the present invention.
In the figure: 1. a case; 101. a fixing plate; 102. a chute; 2. a case cover; 3. a rotating plate; 301. a placement groove; 4. a test tube; 5. a driving rod; 6. a driving device; 7. a housing; 701. a rotary tube; 702. a connecting plate; 703. a first pneumatic telescopic rod; 704. a force-bearing plate; 8. a placing rack; 801. an upper tube body; 8011. a first ring body; 802. a lower pipe body; 8021. a second ring body; 803. a receiving groove; 804. a first elastic element; 9. ear plates; 901. a movable groove; 902. the second pneumatic telescopic rod; 903. a limit clamping block; 10. a sleeve; 1001. a piston; 1002. a second elastic element; 1003. a movable rod; 11. an air guide groove; 12. a moving ring; 121. a swinging rod; 122. a third elastic element; 123. a connecting rod; 124. a slide block; 125. a pull rope; 13. a rubber pad; 131. a bottom cavity; 132. a side cavity; 133. a communication groove; 14. fixing a bevel gear; 141. driven bevel gears.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Example 1:
referring to fig. 1, fig. 2, fig. 5 and fig. 6, a centrifugal device for detecting cell proteins in PCR Array technology comprises a box body 1 and a box cover 2 hinged to the top of the box body 1, wherein a fixing plate 101 is fixedly arranged in the box body 1, a rotating plate 3 is rotatably connected in the fixing plate 101, a placing groove 301 which is uniformly distributed in circumference is arranged on the rotating plate 3, a placing rack 8 is arranged in the placing groove 301, a test tube 4 is arranged on the placing rack 8, a driving rod 5 is fixedly connected on the rotating plate 3, a driving device 6 for driving the driving rod 5 to rotate is arranged in the box body 1, a shock absorbing component is arranged on the outer side of the driving device 6, and a self-positioning component matched with the shock absorbing component is further arranged on the placing rack 8.
Specifically, when equipment is used, place test tube 4 in proper order and get into rack 8 in the standing groove 301, begin to carry out centrifugal operation to test tube 4 afterwards, through controlling the operation of driving equipment 6, driving equipment 6 can be motor or motor etc. can drive actuating lever 5 pivoted equipment, make driving equipment 6 drive actuating lever 5 rotatory, actuating lever 5 drives the rack 8 rotation on rotating plate 3 and rotating plate 3, in this process, the subassembly work of shaking, make actuating lever 5 reduce noise and alleviate vibration when rotating the operation, reduce test tube 4 and lead to the impaired risk of test tube 4 because of the excessive shock force in the centrifugation process, and the subassembly work of shaking is driven to the subassembly of shaking, fix a position test tube 4 top, effectively avoid because the circumstances that the shock force is too big to lead to test tube 4 takes place, guaranteed test tube 4 stability that uses, and then guarantee centrifugal effect.
Example 2:
referring to fig. 2, fig. 5 and fig. 11, a centrifugal device for detecting cell proteins in PCR Array technology, based on embodiment 1, further, the shock absorbing assembly includes a housing 7 fixed inside a case 1 and coated outside a driving device 6, a rotating tube 701 is rotatably connected to an inner wall of the housing 7, a storage cavity is formed between the housing 7 and the rotating tube 701, a liquid is filled in the storage cavity, the rotating tube 701 is fixedly connected to the driving rod 5, a plurality of connection plates 702 are provided outside the rotating tube 701, a first pneumatic expansion rod 703 is connected to the connection plates 702, and a stress plate 704 is connected to an end of the first pneumatic expansion rod 703 far from the connection plates 702.
Specifically, when the driving rod 5 rotates, the driving rod 5 drives the connecting plate 702 to rotate in the shell 7 through the rotating tube 701, vibration is buffered and absorbed by utilizing the damping characteristic of liquid, so that the noise and vibration of the driving rod 5 are reduced during rotation operation, and the risk that the test tube 4 is damaged due to overlarge vibration force in the centrifuging process of the test tube 4 is reduced.
Example 3:
referring to fig. 2, fig. 3, fig. 5, fig. 6, fig. 10 and fig. 11, a centrifugal device for detecting cell proteins in PCR Array technology, based on embodiment 2, further, the self-positioning assembly includes an ear plate 9 fixedly arranged on the placement frame 8, a movable groove 901 is formed in the ear plate 9, a second pneumatic telescopic rod 902 is arranged on the inner wall of the movable groove 901, one end, far away from the inner wall of the movable groove 901, of the second pneumatic telescopic rod 902 is connected with a limiting clamping block 903, and an extrusion inclined plane is arranged on the limiting clamping block 903.
Further, the first pneumatic telescopic rod 703 and the second pneumatic telescopic rod 902 have the same structure and comprise two sleeves 10, the two sleeves 10 are respectively connected with the connecting plate 702 and the inner wall of the movable groove 901, a piston 1001 is slidably connected in each sleeve 10, a second elastic element 1002 is arranged between the piston 1001 and the inner wall of the sleeve 10, and one side of the piston 1001, which is away from the second elastic element 1002, is connected with the movable rod 1003.
Further, the self-positioning assembly further comprises an air guide groove 11 formed in the connecting plate 702, the driving rod 5 and the rotating plate 3, one end of the air guide groove 11 is connected with the first pneumatic telescopic rod 703, and the other end of the air guide groove 11 is connected with the second pneumatic telescopic rod 902 through a hose.
Specifically, the actuating lever 5 drives the connecting plate 702 to rotate in the casing 7 through the rotation pipe 701, the connecting plate 702 drives the atress board 704 to rotate in liquid through the first pneumatic telescopic rod 703, the atress board 704 atress extrudes first pneumatic telescopic rod 703, the air in the first pneumatic telescopic rod 703 gets into the second pneumatic telescopic rod 902 through air guide groove 11 and hose, the second pneumatic telescopic rod 902 is tensile and promotes spacing fixture block 903 and remove in the movable groove 901, make spacing fixture block 903 push the test tube 4 top in the standing groove 301, fix a position test tube 4 top, avoid test tube 4 to receive centrifugal force and fly out when centrifuging, the stability that test tube 4 used has been guaranteed.
Example 4:
referring to fig. 1, 2, 3, 4, 5, 6, 7 and 8, in a centrifugal device for detecting cellular proteins according to the PCR Array technology, further, based on embodiment 1, the placement frame 8 includes an upper tube 801 and a lower tube 802 which are slidably connected, a receiving groove 803 for sliding the upper tube 801 is provided on the lower tube 802, and a first elastic element 804 is provided between an inner wall of the receiving groove 803 and a bottom of the upper tube 801.
Further, the outer sides of the upper pipe body 801 and the lower pipe body 802 are respectively connected with a first ring body 8011 and a second ring body 8021 in a rotating mode, the first ring body 8011 is connected in the placing groove 301 in a rotating mode through a pin shaft, the second ring body 8021 is movably connected with a swinging rod 121, one end, far away from the second ring body 8021, of the swinging rod 121 is movably connected with a moving ring 12, the moving ring 12 is sleeved on the outer side of the driving rod 5, a third elastic element 122 is further sleeved on the outer side of the driving rod 5, two ends of the third elastic element 122 are respectively connected with the moving ring 12 and the shell 7, a connecting rod 123 is connected to the outer side of the moving ring 12, a sliding block 124 is fixedly connected to the connecting rod 123, a sliding groove 102 for the sliding of the sliding block 124 is formed in the box 1, and a pull rope 125 is connected between the sliding block 124 and the box cover 2.
Specifically, when the equipment is used, the case cover 2 is opened, the case cover 2 is turned over relative to the case body 1, pull rope 125 is pulled during the case cover 2 turning over, pull rope 125 drives slide block 124 to move upwards in chute 102, slide block 124 drives movable ring 12 to move upwards in the outside of driving rod 5 through connecting rod 123, third elastic element 122 is stretched, movable ring 12 moves upwards in-process and pulls second ring 8021 through swinging rod 121, second ring 8021 drives lower pipe body 802 to move upwards relative to upper pipe body 801, first elastic element 804 is compressed, and second ring 8021 drives lower pipe body 802 to rotate with the connecting pin shaft of first ring 8011 and of placing groove 301 as the centre of a circle, make placing rack 8 and placing groove 301 be vertical state, then install test tube 4 in different placing rack 8 respectively, because placing rack 8 is shallower and is a straight line with placing groove 301, then the case cover 2 is turned over, case cover 2 is closed again at case body 1 top, case cover 2 no longer produces pull rope 802 relative to upper pipe body 801, the elastic element 802 is pulled down in the third ring 8011, the elastic element is pulled down at the placing rack 802, the area of the second ring body 8021 can be improved, the area of the test tube is increased relative to the place rack 8 is increased, the test tube is moved down by the elastic element is pulled down by the placing rack 8, the test tube is driven by the elastic element is driven down, the test tube is moved down by the test tube 4 is placed down in the placing rack 8, the test tube is placed in the different placing rack 8, and the test tube is placed in the placing rack is placed in the different placing rack 8, and is in the test tube is in the different places, and is in the rack 8, and is in the 5, and is in a line with a line, and is in the 5, and is in the line, and is in the a line, in the 5, and is in the 5, and in the a, and is.
Example 5:
referring to fig. 2 and 9, in a centrifugal apparatus for detecting cellular proteins according to the PCR Array technology, further, in the embodiment 4, a rubber pad 13 is provided on the inner wall of a lower tube 802, a bottom chamber 131 and a side chamber 132 are provided in the rubber pad 13, and a communication groove 133 is provided between the bottom chamber 131 and the side chamber 132.
Specifically, when the test tube 4 is pushed by the limiting clamping block 903, the test tube 4 presses the bottom cavity 131 of the rubber pad 13 in the lower tube body 802, air in the bottom cavity 131 enters the side cavity 132 through the communication groove 133, the side cavity 132 expands to press the rubber pad 13 to the side wall of the test tube 4, the test tube 4 in the center is efficiently fixed, and the use stability of the test tube 4 is guaranteed.
Example 6:
referring to fig. 2, 3, 5 and 6, in a centrifugal device for detecting cellular proteins according to the PCR Array technique, a fixed bevel gear 14 is fixedly connected to a fixed plate 101, and a driven bevel gear 141 engaged with the fixed bevel gear 14 is provided to a rack 8 in accordance with example 4.
Specifically, after the cover 2 is covered, the rack 8 deflects and is obliquely arranged with the placing groove 301, the driven bevel gear 141 on the outer side of the rack 8 is meshed with the fixed bevel gear 14 on the fixed plate 101, when the driving rod 5 drives the rack 8 to rotate through the rotating plate 3, the driven bevel gear 141 on the rack 8 can be meshed with the fixed bevel gear 14 and then rotates, so that the lower tube body 802 and the upper tube body 801 rotate in two ring bodies respectively, and the centrifugal effect on the test tube 4 is further improved.
The invention also discloses a centrifugal method for detecting the cell protein by the PCR Array technology, which comprises the centrifugal equipment for detecting the cell protein by the PCR Array technology, and further comprises the following steps:
s1: when the device is used, the box cover 2 is opened, the box cover 2 is turned over relative to the box body 1, when the box cover 2 is turned over, a pull rope 125 is pulled, the pull rope 125 drives the sliding block 124 to move upwards in the sliding groove 102, the sliding block 124 drives the movable ring 12 to move upwards outside the driving rod 5 through the connecting rod 123, the third elastic element 122 is stretched, the movable ring 12 pulls the second ring 8021 through the swinging rod 121 in the upward moving process, the second ring 8021 drives the lower pipe body 802 to move upwards relative to the upper pipe body 801, the first elastic element 804 is compressed, the second ring 8021 drives the lower pipe body 802 to rotate by taking the connecting pin shaft of the first ring 8011 and the placing groove 301 as the circle center, so that the placing rack 8 and the placing groove 301 are in a vertical state, and then the test tube 4 is respectively installed in different placing racks 8, and the test tube 4 can be quickly placed in the placing rack 8 due to the fact that the placing rack 8 is shallower and is in a straight line with the placing groove 301;
s2: then, the box cover 2 is turned over, so that the box cover 2 is re-closed at the top of the box body 1, the box cover 2 does not generate pulling force on the pull rope 125 any more, the movable ring 12 is reset and moves downwards under the pulling force of the third elastic element 122, the movable ring 12 drives the second ring 8021 to move through the swinging rod 121, the second ring 8021 drives the lower pipe body 802 to deflect relative to the placing groove 301, and the lower pipe body 802 slides relative to the upper pipe body 801, so that the placing rack 8 can accommodate a test tube 4 with a larger area, the area of the test tube 4 leaking out of the placing rack 8 is reduced, and the protection and fixing effects on the test tube 4 are improved;
s3: the driven bevel gear 141 on the outer side of the rack 8 after deflection is meshed with the fixed bevel gear 14 on the fixed plate 101, and when the driving rod 5 drives the rack 8 to rotate through the rotating plate 3, the driven bevel gear 141 on the rack 8 can be meshed with the fixed bevel gear 14 and then rotates, so that the centrifugal effect on the test tube 4 is further improved;
s4: then the driving device 6 is controlled to operate, so that the output end of the driving device 6 drives the driving rod 5 to rotate, and the driving rod 5 drives the placing rack 8 at the placing groove 301 to rotate through the rotating plate 3, so that the cells in the test tube 4 are subjected to centrifugal operation;
s5: when the driving rod 5 rotates, the driving rod 5 drives the connecting plate 702 to rotate in the shell 7 through the rotating tube 701, vibration is buffered and absorbed by utilizing the damping characteristic of liquid, noise and vibration are reduced when the driving rod 5 rotates and operates, the risk of damage to the test tube 4 caused by overlarge vibration force in the centrifuging process of the test tube 4 is reduced, the connecting plate 702 drives the stress plate 704 to rotate in the liquid through the first pneumatic telescopic rod 703, the stress plate 704 is stressed to extrude the first pneumatic telescopic rod 703, air in the first pneumatic telescopic rod 703 enters the second pneumatic telescopic rod 902 through the air guide groove 11 and the hose, and the second pneumatic telescopic rod 902 stretches and pushes the limiting clamping block 903 to move in the movable groove 901, so that the limiting clamping block 903 pushes the top of the test tube 4 in the placing groove 301, and the top of the test tube 4 is positioned;
s6: in the process that the limiting clamping block 903 pushes the test tube 4, the test tube 4 presses the bottom cavity 131 of the rubber pad 13 in the lower tube body 802, air in the bottom cavity 131 enters the side cavity 132 through the communication groove 133, and the side cavity 132 expands to press the rubber pad 13 to the side wall of the test tube 4, so that the test tube 4 in the center is efficiently fixed.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (2)
1. The utility model provides a centrifugal equipment is used in cell protein detection to PCR Array technique, includes box (1) and hinged joint at case lid (2) at box (1) top, a serial communication port, fixed plate (101) have been set firmly in box (1), fixed plate (101) internal rotation is connected with rotor plate (3), be provided with on rotor plate (3) and be circumference evenly distributed's standing groove (301), be provided with rack (8) in standing groove (301), test tube (4) have been placed on rack (8), fixedly connected with actuating lever (5) on rotor plate (3), be provided with in box (1) and be used for driving actuating lever (5) pivoted actuating device (6), actuating device (6) outside is provided with shock absorbing assembly, still be provided with on rack (8) with shock absorbing assembly matched with self-positioning assembly.
The vibration absorbing assembly comprises a shell (7) fixedly arranged inside a box body (1) and coated on the outer side of a driving device (6), a rotating pipe (701) is rotationally connected to the inner wall of the shell (7), a storage cavity is formed between the shell (7) and the rotating pipe (701), liquid is filled in the storage cavity, the rotating pipe (701) is fixedly connected with a driving rod (5), a plurality of connecting plates (702) are arranged on the outer side of the rotating pipe (701), a first pneumatic telescopic rod (703) is connected to the connecting plates (702), and a stress plate (704) is connected to one end, far away from the connecting plates (702), of the first pneumatic telescopic rod (703);
the self-positioning assembly comprises an ear plate (9) fixedly arranged on the placement frame (8), a movable groove (901) is formed in the ear plate (9), a second pneumatic telescopic rod (902) is arranged on the inner wall of the movable groove (901), a limiting clamping block (903) is connected to one end, far away from the inner wall of the movable groove (901), of the second pneumatic telescopic rod (902), and an extrusion inclined plane is arranged on the limiting clamping block (903);
the structure of the first pneumatic telescopic rod (703) and the structure of the second pneumatic telescopic rod (902) are the same, each of the first pneumatic telescopic rod and the second pneumatic telescopic rod comprise a sleeve (10), the two sleeves (10) are respectively connected with the connecting plate (702) and the inner wall of the movable groove (901), a piston (1001) is connected in a sliding manner in each sleeve (10), a second elastic element (1002) is arranged between the piston (1001) and the inner wall of the sleeve (10), and one side, deviating from the second elastic element (1002), of the piston (1001) is connected with the movable rod (1003);
the self-positioning assembly further comprises an air guide groove (11) formed in the connecting plate (702), the driving rod (5) and the rotating plate (3), one end of the air guide groove (11) is connected with the first pneumatic telescopic rod (703), and the other end of the air guide groove (11) is connected with the second pneumatic telescopic rod (902) through a hose;
the placing rack (8) comprises an upper pipe body (801) and a lower pipe body (802) which are connected in a sliding mode, a containing groove (803) used for the sliding of the upper pipe body (801) is formed in the lower pipe body (802), and a first elastic element (804) is arranged between the inner wall of the containing groove (803) and the bottom of the upper pipe body (801);
the novel multifunctional electric power box is characterized in that a first ring body (8011) and a second ring body (8021) are respectively connected to the outer sides of the upper pipe body (801) and the lower pipe body (802) in a rotating mode, the first ring body (8011) is connected in a placing groove (301) in a rotating mode through a pin shaft, a swinging rod (121) is movably connected to the second ring body (8021), one end, far away from the second ring body (8021), of the swinging rod (121) is movably connected with a movable ring (12), the movable ring (12) is sleeved on the outer side of a driving rod (5), a third elastic element (122) is sleeved on the outer side of the driving rod (5), two ends of the third elastic element (122) are connected with the movable ring (12) and a shell (7) respectively, a connecting rod (123) is connected to the outer side of the movable ring (12), a sliding groove (102) used for sliding the sliding of the sliding rod (124) is formed in the box (1), and a pull rope (125) is connected between the sliding groove (124) and a box cover (2).
The inner wall of the lower pipe body (802) is provided with a rubber pad (13), a bottom cavity (131) and a side cavity (132) are formed in the rubber pad (13), and a communication groove (133) is formed between the bottom cavity (131) and the side cavity (132);
fixed bevel gears (14) are fixedly connected to the fixed plate (101), and driven bevel gears (141) meshed with the fixed bevel gears (14) are arranged on the placing frame (8).
2. A centrifugal method for detecting cell proteins by using a PCR Array technology, which comprises the centrifugal device for detecting cell proteins by using the PCR Array technology according to claim 1, and further comprises the following steps:
s1: when the device is used, the box cover (2) is opened, the box cover (2) is turned over relative to the box body (1), a pull rope (125) is pulled when the box cover (2) is turned over, the pull rope (125) drives the sliding block (124) to move upwards in the sliding groove (102), the sliding block (124) drives the movable ring (12) to move upwards outside the driving rod (5) through the connecting rod (123), the third elastic element (122) is stretched, the movable ring (12) pulls the second ring body (8021) through the swinging rod (121) in the upward moving process, the second ring body (8021) drives the lower tube body (802) to move upwards relative to the upper tube body (801), the first elastic element (804) is compressed, the second ring body (8021) drives the lower tube body (802) to rotate by taking a connecting pin shaft of the first ring body (8011) and the placing groove (301) as a circle center, the placing frame (8) and the placing groove (301) are in a vertical state, and then the placing frame (4) is respectively arranged in different placing frames (8), and the placing frame (8) and the placing frame (301) can be placed in a straight line (4) rapidly;
s2: then the box cover (2) is turned over, so that the box cover (2) is re-closed at the top of the box body (1), the box cover (2) does not pull the pull rope (125), the movable ring (12) is reset to move downwards under the pulling of the elastic force of the third elastic element (122), the movable ring (12) drives the second ring body (8021) to move through the swinging rod (121), the second ring body (8021) drives the lower pipe body (802) to deflect relative to the placing groove (301), and the lower pipe body (802) slides relative to the upper pipe body (801), so that the placing rack (8) can accommodate test tubes (4) with larger area, and the area of the test tubes (4) leaking out of the placing rack (8) is reduced, so that the protection and fixing effects on the test tubes (4) are improved;
s3: the driven bevel gear (141) on the outer side of the rack (8) after deflection is meshed with the fixed bevel gear (14) on the fixed plate (101), and when the driving rod (5) drives the rack (8) to rotate through the rotating plate (3), the driven bevel gear (141) on the rack (8) can be meshed with the fixed bevel gear (14) and then rotates;
s4: then, the driving equipment (6) is controlled to operate, the output end of the driving equipment (6) drives the driving rod (5) to rotate, the driving rod (5) drives the placing rack (8) at the placing groove (301) to rotate through the rotating plate (3), and then the cells in the test tube (4) are subjected to centrifugal operation;
s5: when the driving rod (5) rotates, the driving rod (5) drives the connecting plate (702) to rotate in the shell (7) through the rotating pipe (701), vibration is buffered and absorbed by utilizing the damping characteristic of liquid, so that the noise and vibration of the driving rod (5) are reduced when the driving rod rotates and the risk of damage to the test tube (4) caused by overlarge vibration force in the centrifuging process of the test tube (4) is reduced, the connecting plate (702) drives the stress plate (704) to rotate in the liquid through the first pneumatic telescopic rod (703), the stress plate (704) is stressed to extrude the first pneumatic telescopic rod (703), air in the first pneumatic telescopic rod (703) enters the second pneumatic telescopic rod (902) through the air guide groove (11) and a hose, and the second pneumatic telescopic rod (902) stretches and pushes the limiting clamping block (903) to move in the movable groove (901), so that the limiting clamping block (903) pushes the top of the test tube (4) in the placing groove (301) to position the top of the test tube (4);
s6: in the process that limiting clamping blocks (903) push test tubes (4), the test tubes (4) press bottom cavities (131) of rubber pads (13) in lower tube bodies (802), air in the bottom cavities (131) enters side cavities (132) through communication grooves (133), and the side cavities (132) expand to enable the rubber pads (13) to press side walls of the test tubes (4), so that the test tubes (4) in the center are efficiently fixed.
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| CN106076665B (en) * | 2016-08-08 | 2018-07-03 | 安徽惠恩生物科技股份有限公司 | A kind of cell experiment centrifugation systems with test tube mounting bracket |
| CN109453905B (en) * | 2018-12-14 | 2021-03-12 | 任清波 | Blood centrifuge with rotating device |
| CN109794366A (en) * | 2019-01-14 | 2019-05-24 | 上海化工研究院有限公司 | A kind of flexible type micro high speed centrifugation device of umbrella shape |
| CN112439555A (en) * | 2019-09-04 | 2021-03-05 | 瑞莱生物科技(天津)有限公司 | Medical centrifuge with get and put and carry test tube function |
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| CN112221725B (en) * | 2020-09-24 | 2022-04-22 | 湖北中医药高等专科学校 | Vacuum test analysis device for clinical laboratory |
| CN113083520A (en) * | 2021-03-16 | 2021-07-09 | 韩宝云 | Blood centrifuge is used in inspection |
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| CN114405691B (en) * | 2021-12-26 | 2024-06-18 | 山东维尤纳特生物科技有限公司 | Centrifugal equipment for producing metaldehyde |
| CN114308412A (en) * | 2021-12-28 | 2022-04-12 | 杭州华葵金配生物科技有限公司 | Centrifuge for cell processing |
| CN114367386A (en) * | 2021-12-29 | 2022-04-19 | 钟尚军 | Table type centrifuge |
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| CN114655291B (en) * | 2022-03-24 | 2023-10-10 | 弘景智业(北京)多式联运咨询有限公司 | Mechanical automatic conveying device convenient to adjust and conveying method |
| CN115591679B (en) * | 2022-09-27 | 2023-09-01 | 无锡市新吴区新瑞医院(上海交通大学医学院附属瑞金医院无锡分院) | Blood examination centrifugal processor and use method thereof |
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