CN115956569A - Sterilization composition containing methionin and application thereof - Google Patents
Sterilization composition containing methionin and application thereof Download PDFInfo
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- CN115956569A CN115956569A CN202211650625.3A CN202211650625A CN115956569A CN 115956569 A CN115956569 A CN 115956569A CN 202211650625 A CN202211650625 A CN 202211650625A CN 115956569 A CN115956569 A CN 115956569A
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- 239000000203 mixture Substances 0.000 title claims abstract description 32
- 230000001954 sterilising effect Effects 0.000 title claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 title description 4
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 title description 2
- 229930182817 methionine Natural products 0.000 title description 2
- 201000010099 disease Diseases 0.000 claims abstract description 27
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 27
- 150000001412 amines Chemical class 0.000 claims abstract description 24
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 23
- 239000004480 active ingredient Substances 0.000 claims abstract description 8
- 240000008067 Cucumis sativus Species 0.000 claims description 31
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 31
- 241000233679 Peronosporaceae Species 0.000 claims description 21
- OFFFOVCCGHKJES-UHFFFAOYSA-N 4-methylthiadiazole Chemical compound CC1=CSN=N1 OFFFOVCCGHKJES-UHFFFAOYSA-N 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 239000000375 suspending agent Substances 0.000 claims description 7
- 239000004495 emulsifiable concentrate Substances 0.000 claims description 5
- 239000000839 emulsion Substances 0.000 claims description 5
- 239000004563 wettable powder Substances 0.000 claims description 5
- 239000012752 auxiliary agent Substances 0.000 claims description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 claims description 3
- 240000008790 Musa x paradisiaca Species 0.000 claims description 3
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 241000233622 Phytophthora infestans Species 0.000 claims description 3
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 241000723873 Tobacco mosaic virus Species 0.000 claims description 2
- 239000006184 cosolvent Substances 0.000 claims description 2
- 239000002270 dispersing agent Substances 0.000 claims description 2
- 239000003995 emulsifying agent Substances 0.000 claims description 2
- 239000000945 filler Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000004530 micro-emulsion Substances 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000002562 thickening agent Substances 0.000 claims description 2
- 239000004562 water dispersible granule Substances 0.000 claims description 2
- 239000000080 wetting agent Substances 0.000 claims description 2
- 206010039509 Scab Diseases 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000002518 antifoaming agent Substances 0.000 claims 1
- 239000007900 aqueous suspension Substances 0.000 claims 1
- 230000000855 fungicidal effect Effects 0.000 claims 1
- 239000012053 oil suspension Substances 0.000 claims 1
- WOSNCVAPUOFXEH-UHFFFAOYSA-N thifluzamide Chemical compound S1C(C)=NC(C(F)(F)F)=C1C(=O)NC1=C(Br)C=C(OC(F)(F)F)C=C1Br WOSNCVAPUOFXEH-UHFFFAOYSA-N 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 8
- 239000003899 bactericide agent Substances 0.000 abstract description 5
- 238000003912 environmental pollution Methods 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- VZWOXDYRBDIHMA-UHFFFAOYSA-N 2-methyl-1,3-thiazole Chemical compound CC1=NC=CS1 VZWOXDYRBDIHMA-UHFFFAOYSA-N 0.000 abstract 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 15
- 239000003814 drug Substances 0.000 description 13
- 238000012360 testing method Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 230000003902 lesion Effects 0.000 description 10
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 8
- 229960003085 meticillin Drugs 0.000 description 8
- 230000001018 virulence Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 239000000440 bentonite Substances 0.000 description 6
- 229910000278 bentonite Inorganic materials 0.000 description 6
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 6
- 229920005551 calcium lignosulfonate Polymers 0.000 description 6
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 241000222199 Colletotrichum Species 0.000 description 4
- 241001281805 Pseudoperonospora cubensis Species 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000012424 soybean oil Nutrition 0.000 description 4
- 239000003549 soybean oil Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- LWEAHXKXKDCSIE-UHFFFAOYSA-M 2,3-di(propan-2-yl)naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S([O-])(=O)=O)=C(C(C)C)C(C(C)C)=CC2=C1 LWEAHXKXKDCSIE-UHFFFAOYSA-M 0.000 description 3
- 239000005995 Aluminium silicate Substances 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 235000012211 aluminium silicate Nutrition 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 230000000857 drug effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 229920000136 polysorbate Polymers 0.000 description 3
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 3
- 235000010234 sodium benzoate Nutrition 0.000 description 3
- 239000004299 sodium benzoate Substances 0.000 description 3
- 239000000230 xanthan gum Substances 0.000 description 3
- 229920001285 xanthan gum Polymers 0.000 description 3
- 229940082509 xanthan gum Drugs 0.000 description 3
- 235000010493 xanthan gum Nutrition 0.000 description 3
- 239000001871 (1R,2R,5S)-5-methyl-2-prop-1-en-2-ylcyclohexan-1-ol Substances 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 2
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 2
- 241000223195 Fusarium graminearum Species 0.000 description 2
- 241000220225 Malus Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- AMTWCFIAVKBGOD-UHFFFAOYSA-N dioxosilane;methoxy-dimethyl-trimethylsilyloxysilane Chemical compound O=[Si]=O.CO[Si](C)(C)O[Si](C)(C)C AMTWCFIAVKBGOD-UHFFFAOYSA-N 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 229940095045 isopulegol Drugs 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229940068196 placebo Drugs 0.000 description 2
- 239000000902 placebo Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 229940083037 simethicone Drugs 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 240000002066 Amorpha fruticosa Species 0.000 description 1
- 235000004047 Amorpha fruticosa Nutrition 0.000 description 1
- 241001281802 Pseudoperonospora Species 0.000 description 1
- 241000534944 Thia Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- VLLMWSRANPNYQX-UHFFFAOYSA-N thiadiazole Chemical compound C1=CSN=N1.C1=CSN=N1 VLLMWSRANPNYQX-UHFFFAOYSA-N 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of agricultural bactericides, and provides a bactericidal composition containing methyl thiazole induced amine and alpha-isoashbyl, wherein the active ingredients of the bactericidal composition comprise methyl thiazole induced amine and alpha-isoashbyl, and the mass ratio of the methyl thiazole induced amine to the alpha-isoashbyl is 40; the bactericidal composition has excellent synergistic effect on crop diseases; can effectively reduce the usage amount of pesticides, slow down the generation of drug resistance of diseases, reduce environmental pollution and is safe to crops through field observation.
Description
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing methyl thiadiazole induced amine and alpha-isoashbyl and application thereof.
Background
The etiologic agent of cucumber downy mildew is pseudoperonospora cubensis of the genus Pseudoperonospora flagellata, the sporangium of which is spread by airflow and rain. Downy mildew mainly occurs on leaves, the disease occurs in seedling stage, and cotyledons are faded and gradually become yellow irregular spots. The disease occurs in the adult plant stage, light green water-soaked spots appear on leaves, the leaves are mutually limited by veins and are polygonal, yellow green turns brown, the disease spots in the later stage are converged into pieces, the whole leaves are dry, and the leaves of the whole plant die when the disease spots are serious.
The chemical agent is used for preventing and treating cucumber downy mildew, has the advantages of obvious drug effect, convenience and simplicity in use and quick response, but chemical prevention can cause harm to life safety of people and livestock, pesticide residues after use can cause environmental pollution, and the disease resistance is gradually enhanced after long-term use of a single agent. The pesticide compounding is a method for using a bactericide to reduce the generation of pesticide resistance of diseases, and particularly the bactericidal composition with a synergistic effect can delay or overcome the pesticide resistance generated after screening pesticides on pests, reduce the cost and further reduce the environmental safety problem in pesticide use.
The methyl thiadiazole induced amine is a thiadiazole plant disease prevention activator jointly developed and researched by southern university. CN100420686C discloses a synthetic method of thiamine synthase, and also discloses that it has excellent control effects on cucumber fusarium wilt, wheat fusarium graminearum, rice aspergillus oryzae, sclerotinia sclerotiorum, apple rot, banana colletotrichum, cucumber colletotrichum, cotton verticillium wilt and potato late blight; CN101091490a discloses that it has excellent control effect on cucumber downy mildew. The current research result shows that the compound has good effect of inducing disease resistance to virus diseases, and the action mechanism is inducing plants to generate systemic and broad-spectrum disease resistance activity.
Alpha-isoashorin, the chemical structure of which is:
is separated from amorpha fruticosa seeds. China chemical industries, inc. and Shenyang chemical researchThe patent application CN103125503B of the institute Limited company discloses the application of alpha-isogrisein as an agricultural bactericide, and tests that the alpha-isogrisein has an excellent control effect on cucumber downy mildew.
In the prior art, no report is provided about the compounding of methionin and alpha-isobotrytis for controlling cucumber downy mildew.
Disclosure of Invention
The invention provides a sterilization composition with excellent synergistic effect, and the specific technical scheme is as follows:
the active ingredients of the sterilizing composition containing the methyl thiadiazole induced amine consist of the methyl thiadiazole induced amine and alpha-isoashesin, and the mass ratio of the methyl thiadiazole induced amine to the alpha-isoashesin is (40).
The mass ratio of methyl thiadiazole induced amine to alpha-isoashesin in the bactericidal composition is 20.
The mass ratio of the methyl thiadiazole induced amine to the alpha-isoashorin in the bactericidal composition is 1:1-1.
In the bactericidal composition, the mass ratio of the methyl thiadiazole induced amine to the alpha-isoashbyn is 1: 1. 1: 10. 1:20.
the sterilization composition comprises an auxiliary agent, and the weight percentage of the active ingredients is 1-75%. Preferably, the weight percentage of the active ingredients is 10-70%.
The auxiliary agent is one or more of wetting agent, emulsifier, dispersant, suspending agent, antifreezing agent, thickener, defoamer, solvent, cosolvent and filler.
The preparation of the bactericidal composition is missible oil, oil suspending agent, water suspending agent, wettable powder, water dispersible granules, microemulsion, emulsion in water and granules.
The bactericidal composition is used for preventing and treating crop diseases.
The crop diseases comprise cucumber downy mildew, cucumber fusarium wilt, wheat fusarium graminearum, rice aspergillus oryzae, sclerotinia sclerotiorum, apple rot pathogen, banana colletotrichum, cucumber colletotrichum, cotton verticillium wilt, potato late blight and tobacco mosaic virus. Preferably, the crop disease is cucumber downy mildew.
By adopting the technical scheme, the invention has the following beneficial effects:
when the mass ratio of the methicillin-induced amine to the alpha-isoashbyl is 1:1-1. Can effectively reduce the usage amount of pesticides, slow down the generation of drug resistance of diseases and reduce environmental pollution. And is safe to crops in field observation
Detailed Description
The present invention will be further explained with reference to specific embodiments.
Example 1: pot culture test of compounding methicillin-inducing amine and alpha-isopulegol on cucumber downy mildew
Test materials:
pseudoperonospora cubensis (Pseudoperonospora cubensis); collecting and identifying in the field, and culturing and storing the living bodies indoors.
The test method comprises the following steps:
refer to section 7 of "indoor bioassay agricultural chemicals test guidelines for fungicides": test for controlling cucumber downy mildew-pot culture method. Potted cucumber seedlings with the same growth vigor are selected for experiments, and the cucumber seedlings are in a 4-5 leaf stage. At a concentration of 1X 10 6 CFU/mL sporangia suspension was sprayed uniformly onto the back of cucumber leaves with an inoculation sprayer until the surface was wet. After spray inoculation, the mixture is transferred into a climatic chamber, the relative humidity is more than 90 percent, and the temperature is 22 ℃. Spray treatment of the stems and leaves of the preparation is carried out after 24h (concentration gradient of the preparation is determined according to a pre-experiment), and disease condition investigation is carried out after 14 d.
Grading standard:
level 0: no disease;
level 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, stage: the area of the lesion spots accounts for 6 to 10 percent of the area of the whole leaf;
stage 5: the area of the lesion spots accounts for 11 to 25 percent of the area of the whole leaf;
and 7, stage: the area of the lesion spots accounts for 26 to 50 percent of the area of the whole leaf;
and 9, stage: the area of the lesion spots accounts for more than 50 percent of the area of the whole leaf.
And (3) calculating the drug effect:
corresponding regression analysis is carried out according to the concentration of each single agent and the composition and the corresponding control effect to obtain the EC of each agent 50 The value is obtained.
And (3) calculating the co-toxicity coefficient (CTC) of the mixture by using a co-toxicity coefficient calculation method of Sun Yunpei, and determining the synergy of the mixture.
Single dose virulence index = (EC of standard agent) 50 A certain single agent EC 50 )×100；
Theoretical virulence index (TT I) = the virulence index of the A single agent is multiplied by the proportion of the A single agent in the mixed preparation + the virulence index of the B single agent is multiplied by the proportion of the B single agent in the mixed preparation;
measured virulence index (AT I) = (standard single dose EC) 50 EC of value/blend 50 Value) × 100;
co-virulence coefficient (CTC) = (measured virulence index/theoretical virulence index) × 100;
when the CTC is more than 120, the mixture has a synergistic effect, when the CTC is less than 80, antagonism is obtained, and the CTC is added between 80 and 120.
Test results
As can be seen from Table 1, the EC of methicillin against Pseudoperonospora cubensis 50 EC of alpha-isopulegol with value of 8.50mg/L on cucumber downy mildew 50 The value 138.42mg/L; when the ratio of the methicillin to the alpha-isoashbyl is 40: 1. 20: 1. 10: 1. 1: 1. 1: 10. 1:20. 1: EC against cucumber downy mildew at 40 hours 50 The values were 7.80mg/L, 8.25mg/L, 8.73mg/L, 12.75mg/L, 40.47mg/L, 63.41mg/L, 90.05mg/L, respectively.
When the mass ratio of the methicillin-induced amine to the alpha-isoashbyl is 1:1-1; when the mass ratio of the methicillin-induced amine to the alpha-isoashbyl is 40.
Table 1: indoor test for pairing cucumber downy mildew by methicillin-inducing amine and alpha-isoashbya
Example 2: test of field control effect of compounding of methicillin-inducing amine and alpha-isobotrytis on cucumber downy mildew
And (3) testing crop diseases: downy mildew of cucumber
Testing the medicament:
24% emulsifiable solution (containing, for example, 4% of methyl-thia-luramine, 20% of alpha-isoashesin, 500% of agricultural emulsion, 80% of tween, 2% of sodium dodecyl sulfate, 5% of glycol and 100% of soybean oil in percentage by weight);
4% of emulsifiable concentrate (comprising, for example, 4% of methyl-thia-lured amine, 500% of agricultural emulsion, 8% of tween, 4% of sodium dodecyl sulfate, 2% of glycol, 5% of soybean oil and 100% of soybean oil supplement);
20 percent of emulsifiable solution (comprising, for example, 20 percent of alpha-isoashesin, 500 8 percent of agricultural emulsion, 80 percent of tween, 2 percent of sodium dodecyl sulfate, 5 percent of glycol and 100 percent of soybean oil for supplementing foot).
The test method comprises the following steps: refer to the pesticide field efficacy test criterion (I) that the bactericide prevents and treats cucumber downy mildew. Setting a cell: each cell area was 45m2, randomized block permutation, and the experiment was repeated 4 times per treatment. The medicament is applied at the early stage of the onset of cucumber downy mildew, and is sprayed on the leaf surfaces, and the spraying amount is mainly no water drops; the medicine is applied for 2 times at intervals of 7d, the base of the disease condition is investigated before the first medicine application, the disease condition index is investigated 14 days after the second medicine application, and the prevention effect is calculated.
The investigation method comprises the following steps: and randomly surveying 4 points per cell, 2 plants per point, surveying all leaves per plant, and recording the total leaf number and the leaf number of each grade of disease.
Grading standard:
level 0: no disease exists;
stage 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the area of the lesion spots accounts for 6 to 10 percent of the area of the whole leaf;
and 5, stage: the area of the lesion spots accounts for 11 to 25 percent of the area of the whole leaf;
and 7, stage: the area of the lesion spots accounts for 26 to 50 percent of the area of the whole leaf;
and 9, stage: the lesion spot area accounts for more than 50% of the whole leaf area.
And (3) calculating the drug effect:
CK 0-pre-drug disease index for placebo; CK 1-post-drug disease index of placebo zone; PT 0-pre-drug disease index in the drug treatment area; PT 1-post-dose disease index in the drug treatment area.
Test results
As can be seen from the table 2, compared with a single dose, the control effect of the 24 percent compound missible oil diluted by 300 times on cucumber downy mildew is 94.4 percent; the control effect of the compound medicament on the cucumber downy mildew is obviously improved, the use amount of active ingredients can be effectively reduced, the environmental pollution is reduced, and the generation of the drug resistance of diseases is slowed down; and is safe for cucumber observation by adding.
Table 2: test of field control effect of compound medicament on cucumber downy mildew
| Medicament | Dilution factor | Control effect% |
| 24% emulsifiable concentrate | 300 | 94.4 |
| 4% emulsifiable concentrate | 300 | 64.6 |
| 20% emulsifiable concentrate | 300 | 48.2 |
| CK | - | - |
Example 3 formulation example
The 40% wettable powder comprises the following components in percentage by weight:
20% of methyl thiadiazole induced amine, 20% of alpha-isoashitin, 4% of nekal, 5% of sodium dodecyl sulfate, 4% of calcium lignosulfonate, 5% of bentonite and kaolin for 100% of the total.
The 25% wettable powder comprises the following components in percentage by weight:
10% of methyl thiadiazole induced amine, 15% of alpha-isoashitin, 6% of nekal, 2% of sodium dodecyl sulfate, 5% of calcium lignosulfonate, 4% of bentonite and kaolin for 100% of the total.
The 30% wettable powder comprises the following components in percentage by weight:
5% of methyl-thia-luramine, 25% of alpha-isoashesin, 4% of nekal, 5% of sodium dodecyl sulfate, 4% of calcium lignosulfonate, 4% of bentonite and the balance of kaolin to 100%.
35% of suspending agent, which comprises the following components in percentage by weight:
15% of methyl-thia-luramine, 20% of alpha-isoashesin, 4% of calcium lignosulfonate, 4915% of At l ox, 0.5% of xanthan gum, 1.5% of ethylene glycol, 0.5% of sodium benzoate, 0.5% of simethicone, 1% of bentonite and deionized water for making up 100%.
25% of a suspending agent, which comprises the following components in percentage by weight:
10% of methyl thia luramine, 15% of alpha-isoashesin, 5% of calcium lignosulfonate, 4915% of At l ox, 0.8% of xanthan gum, 1.2% of ethylene glycol, 0.5% of sodium benzoate, 0.3% of simethicone, 1.5% of bentonite and deionized water for making up to 100%.
20% of a suspending agent, which comprises the following components in percentage by weight:
10% of methyl thiadiazole induced amine, 10% of alpha-isoashesin, 4% of calcium lignosulfonate, 4915% of At l ox, 0.4% of xanthan gum, 1% of ethylene glycol, 0.5% of sodium benzoate, 0.5% of dimethyl silicone oil, 1.5% of bentonite and deionized water for making up 100%.
Finally, it is also noted that the above-mentioned list is only a few specific embodiments of the present invention. All modifications which can be derived or suggested from the disclosure herein by a person skilled in the art are deemed to be within the scope of the present invention.
Claims (10)
1. The sterilizing composition containing the methyl thiadiazole induced amine is characterized in that the active ingredients comprise the methyl thiadiazole induced amine and alpha-isofolinin, and the mass ratio of the methyl thiadiazole induced amine to the alpha-isofolinin is (40-1).
2. The bactericidal composition according to claim 1, wherein the mass ratio of the thizamide to the α -isoashbyl is 20.
3. The bactericidal composition according to claim 1, wherein the mass ratio of the thifluzamide to the alpha-isoashorin is 1:1-1.
4. The bactericidal composition according to any one of claims 1 to 3, wherein the bactericidal composition comprises the auxiliary agent, and the active ingredient is 1 to 75 weight percent.
5. The bactericidal composition of claim 4, wherein the active ingredients are present in an amount ranging from 10% to 70% by weight.
6. The bactericidal composition of claim 3, wherein the auxiliary agent is one or more of a wetting agent, an emulsifying agent, a dispersing agent, a suspending agent, an antifreezing agent, a thickening agent, an antifoaming agent, a solvent, a cosolvent, and a filler.
7. The bactericidal composition of claim 6, wherein the bactericidal composition is formulated as emulsifiable concentrate, oil suspension, aqueous suspension, wettable powder, water dispersible granules, microemulsion, aqueous emulsion, or granules.
8. Use of the fungicidal composition according to any one of claims 1 to 7 for controlling crop diseases.
9. The use of claim 8, wherein the crop diseases include cucumber downy mildew, cucumber fusarium wilt, wheat scab, rice koji, sclerotinia sclerotiorum, apple rot, banana anthracnose, cucumber anthracnose, cotton verticillium wilt, potato late blight, tobacco mosaic virus.
10. The use according to claim 9, characterized in that the crop disease is cucumber downy mildew.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091490A (en) * | 2007-01-12 | 2007-12-26 | 南开大学 | New type heterocycle bactericide and composition |
| CN103125503A (en) * | 2011-12-02 | 2013-06-05 | 中国中化股份有限公司 | Purpose of alpha-toxicarol serving as agricultural fungicide |
| CN107801720A (en) * | 2016-09-08 | 2018-03-16 | 四川利尔作物科学有限公司 | Bactericidal composition and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091490A (en) * | 2007-01-12 | 2007-12-26 | 南开大学 | New type heterocycle bactericide and composition |
| CN103125503A (en) * | 2011-12-02 | 2013-06-05 | 中国中化股份有限公司 | Purpose of alpha-toxicarol serving as agricultural fungicide |
| CN107801720A (en) * | 2016-09-08 | 2018-03-16 | 四川利尔作物科学有限公司 | Bactericidal composition and its application |
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