CN115926956A - Cell culture exosome collection device - Google Patents
Cell culture exosome collection device Download PDFInfo
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- CN115926956A CN115926956A CN202310070015.4A CN202310070015A CN115926956A CN 115926956 A CN115926956 A CN 115926956A CN 202310070015 A CN202310070015 A CN 202310070015A CN 115926956 A CN115926956 A CN 115926956A
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- China
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- fixed
- cell culture
- exosome
- bottle
- blake bottle
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 46
- 238000004113 cell culture Methods 0.000 title claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 19
- 238000002347 injection Methods 0.000 claims description 18
- 239000007924 injection Substances 0.000 claims description 18
- 238000000502 dialysis Methods 0.000 claims description 13
- 239000000017 hydrogel Substances 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 6
- 235000017491 Bambusa tulda Nutrition 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 6
- 239000011425 bamboo Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 230000001681 protective effect Effects 0.000 description 5
- 210000004748 cultured cell Anatomy 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012930 cell culture fluid Substances 0.000 description 2
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000028023 exocytosis Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The invention provides a cell culture exosome collecting device, and relates to the technical field of cell culture collecting devices. This cell culture exosome collection device, including bottom plate and blake bottle main part, the fixed blake bottle bottom fixing base that is provided with in bottom of blake bottle main part, the top of blake bottle main part is provided with the round along fixed edgewise outward, the fixed round that is provided with in top of going up the edgewise is sealed to fill up, the top of sealing up is provided with down the edgewise, the fixed sealed bottle lid of blake bottle that is provided with in top along the limit down. According to the invention, the rotatable rotating seat is arranged, so that the culture bottle main body can be fixedly arranged on the rotating seat to be fixed when the cell culture exosomes are collected, and then the water in the cell culture exosomes and the exosome liquid can be centrifugally separated through the centrifugal action only by rotating the motor to drive the culture bottle main body to rotate, so that the cell culture exosomes can be better collected.
Description
Technical Field
The invention relates to the technical field of cell culture collection devices, in particular to a cell culture exosome collection device.
Background
The exosome is a common membrane-bound nano vesicle comprising a plurality of biomolecules, is derived from cells through exocytosis, is taken up by target cells, and can transmit biological signals between local or remote cells, and the exosome secretion is a constitutive phenomenon, relates to physiological and pathological processes, determines exosome surface molecules and inclusion, and is still a challenge on how to effectively separate exosomes.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a cell culture exosome collecting device, which can be used for effectively preventing the dialysis filter screen from shaking in the rotating process so as to influence the quality of separated and collected cell culture exosomes by arranging a plurality of fixing holes in the culture bottle main body and fixedly clamping the dialysis filter screen in the culture bottle main body through the fixing holes, and a plurality of purified hydrogel balls are arranged in the dialysis filter screen, so that the cultured cell exosomes can be better adsorbed and collected through the purified hydrogel balls, the collection quality of the cultured cell exosomes is improved, and the separated water in the centrifugal process flows into the bottom of the culture bottle main body through the filter screen and is discharged through a drain hole after the collection is finished.
(II) technical scheme
In order to realize the purpose, the invention is realized by the following technical scheme: the utility model provides a cell culture exosome collection device, includes bottom plate and blake bottle main part, the fixed blake bottle bottom fixing base that is provided with in bottom of blake bottle main part, the top of blake bottle main part is provided with the round outward and is gone up the border, the fixed round that is provided with in top of going up the border is sealed, the sealed top of filling up is provided with down the border, the fixed sealed bottle lid that is provided with of blake bottle of top along the border down, the fixed a plurality of fixed orificess that is provided with in inner wall top of blake bottle main part, it is a plurality of fixed orifices that are fixed on the fixed orifice, fixed joint has solid fixed ring on the fixed orifice, gu fixed ring's the fixed dialysis filter screen that is provided with in bottom, the inside of dialysis filter screen is provided with a plurality of purified water gel balls, the fixed motor box that is provided with in bottom middle part of bottom plate, the fixed motor box's that is provided with in preceding terminal surface is provided with motor switch, the fixed the rotation motor box's that is provided with in inside rotates the motor box, the fixed pivot that is provided with of output, bottom plate fixedly connected with rotates the seat, the top of seat rotates is provided with the round outward and connects the ring.
Preferably, a circle of fixed casing is fixedly arranged on the outer edge of the top end of the bottom plate, a plurality of grooves are fixedly formed in the inner wall of the fixed casing, and a plurality of rotating pulleys are rotatably arranged in each groove;
through above-mentioned technical scheme, the outside of rotating the seat is provided with a fixed section of thick bamboo that protects, and is provided with a plurality of rotation pulleys on the fixed inner wall that protects a section of thick bamboo, can fix the blake bottle main part promptly, and can assist the blake bottle main part to rotate, prevents that the phenomenon of blake bottle main part card on fixed section of thick bamboo that protects from taking place.
Preferably, four supporting columns are fixedly arranged at the bottom of the bottom plate, and supporting seats are fixedly arranged at the bottoms of the four supporting columns.
Preferably, the fixed main joint groove that is provided with in bottom middle part of blake bottle bottom fixing base, the fixed round annular joint groove that is provided with along the limit outside the bottom of blake bottle bottom fixing base, the blake bottle main part passes through main joint groove and the fixed joint in annular joint groove and rotates on the seat.
Preferably, a drain hole is fixedly arranged at the bottom of the side wall of the culture bottle main body, and a sealing nut is arranged on the drain hole;
through above-mentioned technical scheme, the effectual discharge of waste liquid through the wash port.
Preferably, two mounting blocks are fixedly arranged in the middle of the top end of the sealed bottle cap of the culture bottle, and handles are rotatably arranged on the two mounting blocks;
through the technical scheme, the culture bottle main body can be conveniently carried through the handle, and the culture bottle main body is conveniently taken out from the fixed protective cylinder by a worker.
Preferably, a water injection hole is fixedly arranged on one side of the top of the sealing bottle cap of the culture bottle, a water injection pipe is arranged on the top of the water injection hole, an adjusting valve is fixedly arranged on the water injection pipe, and an air hole is fixedly arranged at one end, far away from the water injection hole, of the top of the sealing bottle cap of the culture bottle;
through above-mentioned technical scheme, the top of the sealed bottle lid of blake bottle is provided with the bleeder vent, can strengthen the inside atmospheric pressure of blake bottle main part through the bleeder vent to can accelerate the speed of collecting.
Preferably, a plurality of fastening bolts are arranged between the upper edge of the culture bottle main body and the lower edge of the culture bottle sealing cover;
through above-mentioned technical scheme, through be provided with sealed the pad between sealed bottle lid of blake bottle and blake bottle main part, can strengthen the sealed effect of whole blake bottle main part through sealed the pad, and be provided with a plurality of fixing bolt and can effectually fix sealed bottle lid of blake bottle and blake bottle main part, it is not hard up to prevent that the pivoted in-process from appearing.
The working principle is as follows: this cell culture exosome collection device, at first put into the fixed section of thick bamboo that protects with the blake bottle main part, with rotating the fixed joint of seat, later install the blake bottle main part with the sealed bottle lid of blake bottle again, fix through fixing bolt, fixed back that finishes, irritate the inside of blake bottle main part with cell culture fluid through the water injection pipe, fixed back that finishes, drive the blake bottle main part through the work of change machine and rotate, make cell culture throw away moisture from cell culture fluid through centrifugal action, cell culture exosome is adsorbed to rethread purification hydrogel ball, can collect cell culture exosome.
Advantageous effects
The invention provides a cell culture exosome collecting device. The method has the following beneficial effects:
the invention provides a cell culture exosome collecting device, which is characterized in that a rotatable rotating seat is arranged, a culture bottle main body can be fixedly arranged on the rotating seat to be fixed when cell culture exosomes are collected, then the culture bottle main body is driven to rotate only by rotating a motor, and water in the cell culture exosomes and exosome liquid can be centrifugally separated through centrifugal action, so that the cell culture exosomes can be better collected, a fixed protective cylinder is arranged outside the rotating seat, and a plurality of rotating pulleys are arranged on the inner wall of the fixed protective cylinder, so that the culture bottle main body can be fixed, the culture bottle main body can be assisted to rotate, and the phenomenon that the culture bottle main body is clamped on the fixed protective cylinder is prevented.
The invention provides a cell culture exosome collecting device, which is characterized in that a plurality of fixing holes are formed in a culture bottle main body, a dialysis filter screen is fixedly clamped in the culture bottle main body through the fixing holes, so that the situation that the dialysis filter screen shakes in the rotating process to influence the quality of separated and collected cell culture exosomes can be effectively prevented, a plurality of purified hydrogel balls are arranged in the dialysis filter screen, the cultured cell exosomes can be better adsorbed and collected through the purified hydrogel balls, the collection quality of the cultured cell exosomes is improved, separated water in the centrifuging process flows into the bottom of the culture bottle main body through the filter screen, and the separated water is discharged through a drain hole after the collection is finished.
The invention provides a cell culture exosome collecting device, which is characterized in that a sealing gasket is arranged between a culture bottle sealing bottle cap and a culture bottle main body, the sealing effect of the whole culture bottle main body can be enhanced through the sealing gasket, the culture bottle sealing bottle cap and the culture bottle main body can be effectively fixed through a plurality of fixing bolts, looseness in the rotating process is prevented, air holes are formed in the top of the culture bottle sealing bottle cap, air pressure in the culture bottle main body can be enhanced through the air holes, and therefore the collecting speed can be accelerated.
Drawings
FIG. 1 is a schematic overall view of a cell culture exosome collecting device of the present invention;
FIG. 2 is a front view of a flask body of a cell culture exosome collecting device of the present invention;
FIG. 3 is an isometric view of the flask body of a cell culture exosome collection device of the present invention;
FIG. 4 is a cross-sectional view of the main body of the culture flask of a cell culture exosome collecting device according to the present invention;
FIG. 5 is a cross-sectional view of a stationary casing of a cell culture exosome collecting device of the present invention;
fig. 6 is an enlarged view a in fig. 2.
Wherein, 1, a bottom plate; 2. a support column; 3. a supporting seat; 4. a motor case; 5. a motor switch; 6. fixing the protective cylinder; 7. rotating the motor; 8. a rotating shaft; 9. a rotating seat; 10. a main clamping block; 11. a snap ring; 12. a groove; 13. rotating the pulley; 14. a main culture flask body; 15. the bottom of the culture bottle is fixed; 16. a sealing nut; 17. an upper edge; 18. a fixing hole; 19. a main clamping groove; 20. an annular clamping groove; 21. a fixing ring; 22. a dialysis filter screen; 23. purifying the hydrogel spheres; 24. a drain hole; 25. sealing a bottle cap of the culture bottle; 26. a water injection hole; 27. a water injection pipe; 28. adjusting a valve; 29. air holes are formed; 30. mounting blocks; 31. a handle; 32. a lower edge; 33. fastening a bolt; 34. and a gasket.
Detailed description of the preferred embodiments
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
As shown in fig. 1-6, an embodiment of the present invention provides a cell culture exosome collecting device, including a bottom plate 1 and a culture bottle main body 14, a culture bottle bottom fixing seat 15 is fixedly disposed at the bottom of the culture bottle main body 14, a circle of upper edge 17 is fixedly disposed at the top outer edge of the culture bottle main body 14, a circle of sealing gasket 34 is fixedly disposed at the top of the upper edge 17, a lower edge 32 is disposed at the top of the sealing gasket 34, a culture bottle sealing bottle cap 25 is fixedly disposed at the top of the lower edge 32, a plurality of fixing holes 18 are fixedly disposed at the top of the inner wall of the culture bottle main body 14, a fixing ring 21 is fixedly clamped on the plurality of fixing holes 18, a dialysis filter net 22 is fixedly disposed at the bottom of the fixing ring 21, a plurality of purified hydrogel balls 23 are disposed inside the dialysis filter net 22, a motor box 4 is fixedly disposed at the middle portion of the bottom end of the bottom plate 1, a motor switch 5 is fixedly disposed at the front end face of the motor box 4, a rotating motor 7 is fixedly disposed inside the motor box 4, a rotating motor 7 is fixedly disposed at the output end of the rotating motor 7, a rotating shaft 8 is fixedly disposed at the top of the rotating motor 8, a rotating block 10 is fixedly connected to the top of the rotating seat 9, and a circle of the rotating block 11 is disposed at the top fixedly connected to the rotating seat 9.
The top of bottom plate 1 is provided with the fixed a section of thick bamboo 6 that protects of round along fixed being provided with outward, and a plurality of recesses 12 have been fixed to have seted up on the fixed inner wall that protects a section of thick bamboo 6, all rotates in every recess 12 and is provided with a plurality of rotating pulley 13. The bottom of bottom plate 1 is fixed and is provided with four support columns 2, and the bottom of four support columns 2 is all fixed and is provided with supporting seat 3. The fixed main joint groove 19 that is provided with in bottom middle part of blake bottle bottom fixing base 15, the outer border of bottom of blake bottle bottom fixing base 15 is fixed and is provided with round annular joint groove 20, and blake bottle main part 14 is on rotating seat 9 through the fixed joint of main joint groove 19 and annular joint groove 20. A drain hole 24 is fixedly arranged at the bottom of the side wall of the culture bottle main body 14, and the sealing screw cap 16 is arranged on the drain hole 24. Two installation blocks 30 are fixedly arranged in the middle of the top end of the sealed bottle cap 25 of the culture bottle, and a handle 31 is rotatably arranged on the two installation blocks 30. The top one side of the sealed bottle lid 25 of blake bottle is fixed and is provided with water injection hole 26, and the top of water injection hole 26 is provided with water injection pipe 27, and the last fixed governing valve 28 that is provided with of water injection hole 28, the top of the sealed bottle lid 25 of blake bottle is kept away from the fixed bleeder vent 29 that is provided with of one end of water injection hole 26, is provided with a plurality of fastening bolt 33 between the last border 17 of blake bottle main part 14 and the lower border 32 of the sealed bottle lid 25 of blake bottle.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A cell culture exosome collecting device, includes bottom plate (1) and blake bottle main part (14), its characterized in that: the bottom of blake bottle main part (14) is fixed and is provided with blake bottle bottom fixing base (15), the top of blake bottle main part (14) is provided with a circle of border (17) along the fixed reason outward, the fixed sealed pad (34) of round that is provided with in top of going up border (17), the top of sealed pad (34) is provided with down border (32), the fixed sealed bottle lid (25) of blake bottle that is provided with in top of border (32) down, the fixed a plurality of fixed orificess (18) that are provided with in inner wall top of blake bottle main part (14), it is a plurality of fixed orifices (18), a plurality of fixed joint has solid fixed ring (21) on fixed orifice (18), the fixed dialysis filter screen (22) that is provided with in bottom of solid fixed ring (21), the inside of dialysis filter screen (22) is provided with a plurality of purification hydrogel balls (23), the fixed motor box (4) that is provided with in the bottom middle part of bottom (1), the preceding terminal surface of motor box (4) is fixed and is provided with motor switch (5), the fixed rotation motor (7) that is provided with in the inside of motor box (4), the fixed rotation motor (7) that is provided with the output of rotation motor (7), the fixed pivot (8) passes pivot (8), the fixed top of pivot (9) is connected with rotation block (9) top of rotation block (10), and a circle of clamping ring (11) is fixedly arranged on the outer edge of the top of the rotating seat (9).
2. A cell culture exosome collecting device according to claim 1, characterised in that: the top of bottom plate (1) is provided with a round fixed casing (6) along fixed outward, a plurality of recesses (12) have been fixed to be seted up on the inner wall of fixed casing (6), every all rotate in recess (12) and be provided with a plurality of rotating pulley (13).
3. A cell culture exosome collection device according to claim 1, characterised in that: the bottom of bottom plate (1) is fixed and is provided with four support columns (2), four the bottom of support column (2) all is fixed and is provided with supporting seat (3).
4. A cell culture exosome collecting device according to claim 1, characterised in that: the bottom middle part of blake bottle bottom fixing base (15) is fixed and is provided with main joint groove (19), the fixed round annular joint groove (20) that is provided with along the limit outside the bottom of blake bottle bottom fixing base (15), blake bottle main part (14) are on rotating seat (9) through main joint groove (19) and the fixed joint in annular joint groove (20).
5. A cell culture exosome collecting device according to claim 1, characterised in that: a drain hole (24) is fixedly arranged at the bottom of the side wall of the culture bottle main body (14), and a sealing screw cap (16) is arranged on the drain hole (24).
6. A cell culture exosome collection device according to claim 1, characterised in that: two installation blocks (30) are fixedly arranged in the middle of the top end of the sealed bottle cap (25) of the culture bottle, and a lifting handle (31) is rotatably arranged on each installation block (30).
7. A cell culture exosome collection device according to claim 1, characterised in that: the fixed water injection hole (26) that is provided with in top one side of the sealed bottle lid of blake bottle (25), the top of water injection hole (26) is provided with water injection pipe (27), the fixed governing valve (28) that is provided with on water injection pipe (27), the fixed bleeder vent (29) that is provided with in one end that water injection hole (26) were kept away from at the top of the sealed bottle lid of blake bottle (25).
8. A cell culture exosome collecting device according to claim 1, characterised in that: a plurality of fastening bolts (33) are arranged between the upper edge (17) of the culture bottle main body (14) and the lower edge (32) of the culture bottle sealing cover (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310070015.4A CN115926956A (en) | 2023-02-07 | 2023-02-07 | Cell culture exosome collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310070015.4A CN115926956A (en) | 2023-02-07 | 2023-02-07 | Cell culture exosome collection device |
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| Publication Number | Publication Date |
|---|---|
| CN115926956A true CN115926956A (en) | 2023-04-07 |
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ID=86699308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310070015.4A Pending CN115926956A (en) | 2023-02-07 | 2023-02-07 | Cell culture exosome collection device |
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| Country | Link |
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| CN (1) | CN115926956A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100184200A1 (en) * | 2009-01-22 | 2010-07-22 | Tatung University | Cell culture device |
| CN209577053U (en) * | 2019-02-20 | 2019-11-05 | 林嘉盈 | The extraction element of excretion body separation in a kind of umbilical cord stem cells culture supernatant |
| CN211771366U (en) * | 2020-01-05 | 2020-10-27 | 天津久益生物科技有限公司 | Screw-mouth cell culture bottle |
| CN213855123U (en) * | 2020-11-16 | 2021-08-03 | 南京紫金山分子医学技术研究院有限公司 | Full-automatic exosome extraction element |
| CN215209491U (en) * | 2021-04-28 | 2021-12-17 | 武汉华洋细胞技术有限公司 | Cell culture and exosome collecting device |
| CN215799511U (en) * | 2021-06-07 | 2022-02-11 | 河南省银丰生物工程技术有限公司 | A extraction element for cell exosome |
| WO2022212462A1 (en) * | 2021-03-31 | 2022-10-06 | Corning Incorporated | Cell culture device sensor caps, systems, and methods |
| CN217628424U (en) * | 2022-05-18 | 2022-10-21 | 杨光华 | Novel cell culture device for continuously collecting cell exosomes |
| WO2023134476A1 (en) * | 2022-01-12 | 2023-07-20 | 阜阳师范大学 | Animal cell tissue culture device and culture method |
-
2023
- 2023-02-07 CN CN202310070015.4A patent/CN115926956A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100184200A1 (en) * | 2009-01-22 | 2010-07-22 | Tatung University | Cell culture device |
| CN209577053U (en) * | 2019-02-20 | 2019-11-05 | 林嘉盈 | The extraction element of excretion body separation in a kind of umbilical cord stem cells culture supernatant |
| CN211771366U (en) * | 2020-01-05 | 2020-10-27 | 天津久益生物科技有限公司 | Screw-mouth cell culture bottle |
| CN213855123U (en) * | 2020-11-16 | 2021-08-03 | 南京紫金山分子医学技术研究院有限公司 | Full-automatic exosome extraction element |
| WO2022212462A1 (en) * | 2021-03-31 | 2022-10-06 | Corning Incorporated | Cell culture device sensor caps, systems, and methods |
| CN215209491U (en) * | 2021-04-28 | 2021-12-17 | 武汉华洋细胞技术有限公司 | Cell culture and exosome collecting device |
| CN215799511U (en) * | 2021-06-07 | 2022-02-11 | 河南省银丰生物工程技术有限公司 | A extraction element for cell exosome |
| WO2023134476A1 (en) * | 2022-01-12 | 2023-07-20 | 阜阳师范大学 | Animal cell tissue culture device and culture method |
| CN217628424U (en) * | 2022-05-18 | 2022-10-21 | 杨光华 | Novel cell culture device for continuously collecting cell exosomes |
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