CN115926942A - Nucleic acid extraction instrument - Google Patents
Nucleic acid extraction instrument Download PDFInfo
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- CN115926942A CN115926942A CN202310245596.0A CN202310245596A CN115926942A CN 115926942 A CN115926942 A CN 115926942A CN 202310245596 A CN202310245596 A CN 202310245596A CN 115926942 A CN115926942 A CN 115926942A
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- medicine
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 61
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 61
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 61
- 238000000605 extraction Methods 0.000 title description 13
- 238000012360 testing method Methods 0.000 claims abstract description 77
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 56
- 239000003814 drug Substances 0.000 claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 48
- 230000000712 assembly Effects 0.000 claims abstract description 5
- 238000000429 assembly Methods 0.000 claims abstract description 5
- 239000011324 bead Substances 0.000 claims description 25
- 239000000243 solution Substances 0.000 claims description 21
- 239000012530 fluid Substances 0.000 claims description 16
- 238000001802 infusion Methods 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 8
- 239000012149 elution buffer Substances 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000013589 supplement Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 description 5
- 238000007599 discharging Methods 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 230000001502 supplementing effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 238000005336 cracking Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of genetic engineering instruments and equipment, and particularly discloses a nucleic acid extractor which comprises a base, a movable dosing assembly, a test tube bin module and a special test tube, wherein the test tube bin module is relatively fixed with the base; the number of the special test tubes is multiple, and the special test tubes are placed in the test tube bin module; the number of the movable dosing assemblies is multiple, and each movable dosing assembly is filled with different reagents and used for adding different reagents into the special test tubes; the special test tube is provided with a first liquid discharge port which can discharge liquid medicine. The test tube bin module is not required to be prefabricated with various reagents to be used, and can be used for placing nucleic acid samples to be extracted, so that the problem that the detection efficiency is seriously influenced because the nucleic acid extractor can only extract a small amount of nucleic acid samples at one time because the reagents to be used of the conventional nucleic acid extractor occupy a large amount of space of the reagent bin module is solved.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to a nucleic acid extraction instrument.
Background
The nucleic acid extractor is an instrument which automatically finishes the work of extracting the nucleic acid of a sample by using a matched nucleic acid extracting reagent. Nucleic acid extraction comprises the following basic steps: 1. cracking: adding lysis solution into the sample, and mechanically moving and heating to uniformly mix and fully react the reaction solution, so that cells are lysed, and nucleic acid is released; 2. adsorption: adding magnetic beads into a sample lysis solution, fully and uniformly mixing, adsorbing nucleic acid by utilizing the characteristic that the magnetic beads have strong affinity to the nucleic acid under high salt and low pH values, and separating the magnetic beads from the lysis solution under the action of an external magnetic field; 3. washing: transferring the magnetic beads adsorbed with the nucleic acid into a new washing buffer solution, fully and uniformly mixing, washing away impurities, and separating the magnetic beads from the washing buffer solution under the action of an external magnetic field; 4. and (3) elution: and transferring the magnetic beads into an elution buffer, removing the external magnetic field to fully and uniformly mix the magnetic beads and the eluent, separating the combined nucleic acid from the magnetic beads, and mixing the nucleic acid into the elution buffer to obtain the purified nucleic acid.
The existing nucleic acid extraction instrument generally adopts a magnetic bead separation technology of a magnetic rod method, realizes the adsorption and transfer of magnetic beads by utilizing the mutual matching motion of a magnetic rod and a magnetic rod sleeve, simultaneously utilizes the magnetic rod sleeve to repeatedly stir liquid, fully mixes a reaction liquid system, and finally obtains purified nucleic acid through steps of sample cracking, nucleic acid adsorption to the magnetic beads, magnetic bead washing, nucleic acid elution and the like. In a nucleic acid extractor, a magnetic rod and a magnetic rod sleeve are respectively and directly connected with two power elements, and the magnetic rod sleeve are synchronously moved, synchronously vibrated, independently moved or vibrated by respective direct drive of the two power elements.
For example, chinese patent publication No. CN113462557B discloses a nucleic acid extraction instrument, wherein the driving assembly includes a magnetic rod assembly and a magnetic sleeve assembly, the magnetic rod assembly and the magnetic sleeve assembly are disposed above the heating assembly, the magnetic sleeve assembly includes a magnetic rod sleeve, the magnetic rod sleeve is connected with a magnetic sleeve driving structure, the magnetic sleeve driving structure is directly connected with a second driving assembly, and the second driving assembly directly drives the magnetic sleeve driving structure to move; the bar magnet subassembly includes the bar magnet, be connected with bar magnet bearing structure on the bar magnet, the extrusion is provided with the actuating arm on the bar magnet bearing structure, the below of actuating arm is provided with bar magnet drive structure, bar magnet drive structure and first drive assembly lug connection, the surface of bar magnet drive structure is connected for the contact with the surface of actuating arm, first drive assembly drive bar magnet drive structure rebound, the indirect actuating arm that supports drives the bar magnet bearing structure and shifts up, bar magnet bearing structure relies on bar magnet subassembly self gravity downstream. The nucleic acid extractor has safe, reliable and difficult damage driving effect, small volume and high heating efficiency; the heating efficiency of the heating assembly is improved, the heat radiation which is not beneficial to the nucleic acid extraction effect is reduced, the excessive volatilization of the reagent is reduced, the stability of the concentration of the reagent is ensured, and the efficiency and the extraction quality of the nucleic acid extraction are improved; the assembly precision of the heating components is high, the position deviation or the up-and-down dislocation is not easy to occur, the matching precision of the kit and the heating device is high, and the improvement of the quality and the efficiency of nucleic acid extraction is facilitated.
However, the above-mentioned technical solutions have the following problems: besides the nucleic acid samples to be extracted, the reagent bin module is also preset with reagents such as lysis solution, magnetic bead solution, ground washing buffer solution and elution buffer solution which need to be used in sequence, and the reagents occupy a large amount of space, so that the nucleic acid extraction instrument can only extract a small amount of nucleic acid samples at a time, and the detection efficiency is seriously influenced.
Disclosure of Invention
The invention provides a nucleic acid extractor, and aims to solve the problem that reagents to be used in the existing nucleic acid extractor occupy a large amount of space of a reagent bin module, so that the nucleic acid extractor can only extract a small amount of nucleic acid samples at one time, and the detection efficiency is seriously influenced.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a nucleic acid extractor comprises a base, a movable dosing assembly, a test tube bin module and a special test tube, wherein the test tube bin module is relatively fixed with the base; the number of the special test tubes is multiple, and the special test tubes are placed in the test tube bin module; the number of the movable dosing assemblies is multiple, and each movable dosing assembly is filled with different reagents and used for adding different reagents into the special test tubes; the special test tube is provided with a first liquid discharge port which can discharge liquid medicine.
By adopting the technical scheme, various reagents to be used do not need to be prefabricated in the test tube bin module, the reagents to be used can be completely used for placing nucleic acid samples to be extracted, and the problem that the existing nucleic acid extraction instrument only can extract a small amount of nucleic acid samples at a time and the detection efficiency is seriously influenced because the reagents to be used occupy a large amount of space of the reagent bin module is solved. Different reagents are contained in each movable medicine adding assembly, the liquid medicine is stored respectively, mixed pollution is avoided, the last movable medicine adding assembly does not influence the operation of the next movable medicine adding assembly, even if the last movable medicine adding assembly does not add medicine, the next movable medicine adding assembly can start to add medicine in a new round, and the nucleic acid extraction efficiency is improved.
Further, the test tube bin module includes:
the vibrating table is arranged on the base; a plurality of vibrating tables are arranged on the vibrating table
And the placing component is used for placing the special test tubes.
Further, the placement assembly includes:
the electromagnetic rod can generate and eliminate magnetic force by electrifying and powering off;
the heater is used for heating the liquid medicine in the special test tube;
and the second liquid discharge port is connected with the first liquid discharge port.
Further, the number of the vibration tables is plural.
Further, a track is arranged on the base; remove the medicine subassembly and include:
the moving device runs on the track;
the mounting rack is fixed on the mobile device;
the reagent bins are arranged on the mounting frame; each reagent bin is provided with a liquid outlet;
further, near the track be provided with fluid infusion mouth, when removing the medicine subassembly and remove to fluid infusion mouth position, fluid infusion mouth can be to removing the reagent of supplementing in the medicine subassembly.
Furthermore, the liquid supplementing opening is formed in the base, a runner is formed in the moving device and the mounting frame of the movable medicine adding assembly, and reagents enter the reagent bin through the runner from the liquid supplementing opening.
By adopting the technical scheme, the medicine adding component can be conveniently moved to automatically supplement liquid without manual addition.
The use method of the nucleic acid extractor comprises the following steps: placing a nucleic acid sample to be extracted into a special test tube of a test tube bin module, moving a dosing assembly to sequentially add a first reagent into the special test tube, after the reaction is finished, retaining magnetic beads, discharging waste liquid, then moving the dosing assembly to sequentially add a second reagent into the special test tube, repeating the steps until elution buffer is finally added, fully mixing, retaining the magnetic beads, and collecting discharged liquid medicine, wherein the liquid medicine is nucleic acid extracting solution.
Drawings
FIG. 1 is a diagram showing the construction of a nucleic acid extracting apparatus according to an embodiment of the present invention;
FIG. 2 is a block diagram of a placement module according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a specially-produced test tube according to an embodiment of the present invention.
In the figure: 100-base, 110-track, 120-fluid infusion port, 200-mobile medicine feeding component, 210-mobile device, 220-mounting rack, 230-reagent bin, 300-test tube bin module, 310-vibration table, 320-placing component, 321-electromagnetic rod, 322-heater, 323-second fluid outlet, 400-purpose-made test tube and 410-first fluid outlet.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure. While the invention will be described in conjunction with the preferred embodiments, it is not intended that features of the invention be limited to these embodiments. On the contrary, the invention is described in connection with the embodiments for the purpose of covering alternatives or modifications that may be extended based on the claims of the present invention. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be practiced without these particulars. Moreover, some of the specific details have been left out of the description in order to avoid obscuring or obscuring the focus of the present invention. It should be noted that the embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
It should be noted that in this specification, like reference numerals and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the present invention are used, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have specific orientations, be configured in specific orientations, and operate, and thus, should not be construed as limiting the present invention.
The terms "first," "second," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
In the description of the present embodiment, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected," and "connected" are to be interpreted broadly, e.g., as a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present embodiment can be understood in specific cases by those of ordinary skill in the art.
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Referring to fig. 1 and 3, the present embodiment discloses a nucleic acid extractor, which includes a base 100, a mobile medicine-adding assembly 200, a test tube bin module 300, and a special test tube 400. The test tube bin module 300 is relatively fixed with the base 100, a plurality of special test tubes 400 are arranged in the test tube bin module 300, and the movable medicine adding assembly 200 runs on the base 100 and adds medicine into the special test tubes 400. The special test tube 400 is provided with a first liquid discharge port 410 for discharging liquid medicine.
The principle and the using method of the embodiment are as follows: placing a nucleic acid sample to be extracted into a special test tube 400 of a test tube bin module 300, moving a medicine adding assembly 200 to sequentially add a first reagent into the special test tube 400, after the reaction is finished, keeping magnetic beads, discharging waste liquid, then moving the medicine adding assembly 200 to sequentially add a second reagent into the special test tube 400, repeating the steps until elution buffer solution is finally added, fully mixing, keeping the magnetic beads, and collecting discharged liquid medicine, wherein the liquid medicine is nucleic acid extracting solution.
Further, referring to fig. 2, the test tube bin module 300 includes a plurality of vibration tables 310, and the vibration tables 310 are mounted on the base 100. Each vibration table 310 is provided with a plurality of placing assemblies 320 for placing the special test tubes 400. The placing assembly 320 comprises an electromagnetic rod 321, a heater 322 and a second liquid outlet 323, wherein the electromagnetic rod 321 can generate and eliminate magnetic force by being powered on and off, the heater 322 is used for heating the liquid medicine in the special test tube 400, and the second liquid outlet 323 is connected with the first liquid outlet 410 and used for discharging the liquid medicine.
Further, be provided with track 110 on the base 100, remove medicine subassembly 200 and include mobile device 210, be fixed with mounting bracket 220 on the mobile device 210, install a plurality of reagent storehouses 230 on the mounting bracket 220, be provided with the liquid outlet on every reagent storehouse 230. The moving device 210 moves along the track 110, so that the reagent bin 230 moves above the special test tube 400, and when the reagent bin moves to a position right above the specified special test tube 400, the reagent bin adds the reagent into the special test tube 400 through the liquid outlet. Then the reagent bin 230 moves to the position right above other special test tubes 400 to add medicine to other special test tubes 400.
Further, the number of the mobile medicine-adding assemblies 200 is multiple, and the reagent bin 230 of each mobile medicine-adding assembly 200 contains different reagents for adding different reagents into the special test tube 400.
Further, a fluid infusion port 120 is disposed near the rail 110, and when the mobile medicine adding assembly 200 moves to the position of the fluid infusion port 120, the fluid infusion port 120 can replenish the reagent in the reagent chamber 230 of the mobile medicine adding assembly 200. Further, the fluid infusion port 120 is opened on the base 100, a flow channel is formed in the moving device 210 and the mounting rack 220 of the mobile medicine adding assembly 200, and the reagent enters the reagent bin 230 through the flow channel from the fluid infusion port 120.
The specific application method of this embodiment is as follows:
the special test tubes 400 are placed on the placing component 320 in the test tube bin module 300, and each special test tube 400 is added with a nucleic acid sample to be extracted. A movable medicine adding assembly 200 sequentially adds lysis solution into each special test tube 400, when the special test tubes 400 on a vibration table 310 are added with medicine, the vibration table 310 starts to vibrate, and a heater 322 heats the special test tubes 400 to crack cells and release nucleic acid. The mobile medicated assembly 200 continues to move, heat the special test tubes 400 on the other vibration tables 310, and repeat the above steps. When the liquid medicine on the first vibration table 310 is cracked, the heating is stopped, the second mobile medicine-adding assembly 200 starts to operate, and the magnetic bead liquid is added into the special test tube 400 on the vibration table 310 and is continuously vibrated and mixed. After the dosing of the special test tube 400 on the first vibrating table 310 is completed, the next vibrating table 310 is cracked, and the second mobile dosing assembly 200 continues to dose the special test tube 400 on the second vibrating table 310. When the liquid medicine on the first vibration table 310 finishes vibration mixing, the vibration table 310 stops vibrating, the electromagnetic rod 321 is electrified and magnetized, the magnetic beads are kept still and adsorbed until the magnetic beads are adsorbed by the electromagnetic rod 321 and tightly attached to the inner wall of the special test tube 400, and the first liquid discharge port 410 and the second liquid discharge port 323 are opened to discharge waste liquid. Then the first liquid outlet 410 and the second liquid outlet 323 are closed, the electromagnetic rod 321 is closed, the third mobile medicine adding assembly 200 starts to add washing buffer solution to the special test tube 400 on each vibration table 310 in sequence, and each vibration table 310 starts to mix in sequence. After mixing, the vibration table 310 is stopped in sequence, the electromagnetic rod 321 is opened, the magnetic beads are kept still and adsorbed until the magnetic beads are adsorbed by the electromagnetic rod 321 and tightly attached to the inner wall of the special test tube 400, and the first liquid outlet 410 and the second liquid outlet 323 are opened to discharge waste liquid. Then adding elution buffer solution in the same way, mixing, standing, adsorbing, finally collecting the discharged liquid medicine, namely the nucleic acid extracting solution, and collecting and cleaning the rest special test tubes 400 with magnetic beads or directly treating the special test tubes as waste consumables. When the reagent amount in the reagent bin 230 of the movable dosing assembly 200 is insufficient, the movable dosing assembly 200 returns to the corresponding dosing port to replenish the liquid medicine.
The present invention has been described in detail with reference to the preferred embodiments thereof, and it should be understood that the present invention is not limited thereto, but includes any modifications, equivalents, and improvements within the spirit and scope of the present invention.
Claims (8)
1. A nucleic acid extractor comprises a base (100), a movable medicine adding assembly (200), a test tube bin module (300) and a special test tube (400), and is characterized in that the test tube bin module (300) is relatively fixed with the base (100); the number of the special test tubes (400) is multiple, and the special test tubes are placed in the test tube bin module (300); the number of the mobile medicine adding assemblies (200) is multiple, and different reagents are contained in each mobile medicine adding assembly (200) and are used for adding different reagents into the special test tubes (400); the special test tube (400) is provided with a first liquid discharge port (410) which can discharge liquid medicine.
2. The nucleic acid extractor of claim 1, wherein the tube magazine module (300) comprises:
a vibration table (310) mounted on the base (100); a plurality of vibrating tables (310) are arranged on the vibrating table
A placement assembly (320) for placing the purpose-made test tubes (400).
3. The nucleic acid extractor of claim 2, wherein the placement module (320) comprises:
an electromagnetic rod (321), the electromagnetic rod (321) being capable of generating and eliminating a magnetic force by being energized and de-energized;
the heater (322) is used for heating the liquid medicine in the special test tube (400);
and a second drain port (323) connected to the first drain port (410).
4. The nucleic acid extractor of claim 2, wherein the number of the vibration table (310) is plural.
5. The nucleic acid extractor of claim 1, wherein the base (100) is provided with a rail (110); the mobile medicated assembly (200) comprises:
a moving device (210) running on the track (110);
a mounting rack (220) fixed on the mobile device (210);
a plurality of reagent bins (230), wherein the reagent bins (230) are mounted on the mounting rack (220); each reagent bin (230) is provided with a liquid outlet.
6. The nucleic acid extractor according to claim 5, wherein a fluid infusion port (120) is disposed near the rail (110), and when the mobile medicine adding assembly (200) moves to the position of the fluid infusion port (120), the fluid infusion port (120) can supplement a reagent into the mobile medicine adding assembly (200).
7. The nucleic acid extractor of claim 6, wherein the fluid infusion port (120) is opened on the base (100), and the moving device (210) and the mounting rack (220) of the mobile medicine-adding assembly (200) have flow channels formed therein, through which the reagent passes from the fluid infusion port (120) into the reagent chamber (230).
8. The use method of the nucleic acid extractor as claimed in claim 1, wherein the nucleic acid sample to be extracted is placed in a special test tube (400) of the test tube bin module (300), the mobile medicine-adding assembly (200) sequentially adds a first reagent into the special test tube (400), after the reaction is completed, the magnetic beads are retained, waste liquid is discharged, then the mobile medicine-adding assembly (200) sequentially adds a second reagent into the special test tube (400), the steps are repeated until an elution buffer solution is finally added, the magnetic beads are retained after the complete mixing, and the discharged liquid medicine is collected, wherein the liquid medicine is the nucleic acid extracting solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310245596.0A CN115926942A (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310245596.0A CN115926942A (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction instrument |
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| CN115926942A true CN115926942A (en) | 2023-04-07 |
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| CN202310245596.0A Pending CN115926942A (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction instrument |
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2023
- 2023-03-15 CN CN202310245596.0A patent/CN115926942A/en active Pending
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Application publication date: 20230407 |