CN115918654A - Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice - Google Patents
Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice Download PDFInfo
- Publication number
- CN115918654A CN115918654A CN202211618080.8A CN202211618080A CN115918654A CN 115918654 A CN115918654 A CN 115918654A CN 202211618080 A CN202211618080 A CN 202211618080A CN 115918654 A CN115918654 A CN 115918654A
- Authority
- CN
- China
- Prior art keywords
- extract
- composition
- herbicides
- crops
- quassia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004009 herbicide Substances 0.000 title claims abstract description 53
- 239000000284 extract Substances 0.000 title claims abstract description 48
- 235000009694 Quassia amara Nutrition 0.000 title claims abstract description 40
- 229940013788 quassia Drugs 0.000 title claims abstract description 40
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 30
- 235000009566 rice Nutrition 0.000 title claims abstract description 30
- 231100000674 Phytotoxicity Toxicity 0.000 title claims abstract description 24
- 239000005556 hormone Substances 0.000 title claims abstract description 23
- 229940088597 hormone Drugs 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 11
- 240000007594 Oryza sativa Species 0.000 title abstract 2
- 240000003085 Quassia amara Species 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 claims abstract description 33
- 230000002363 herbicidal effect Effects 0.000 claims abstract description 25
- 238000010298 pulverizing process Methods 0.000 claims abstract description 5
- 238000007873 sieving Methods 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 4
- 241000158615 Quassia Species 0.000 claims description 38
- 241000209094 Oryza Species 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 15
- 239000002689 soil Substances 0.000 claims description 14
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- -1 isooctyl Chemical group 0.000 claims description 9
- 150000002148 esters Chemical class 0.000 claims description 8
- OLZQTUCTGLHFTQ-UHFFFAOYSA-N octan-2-yl 2-(4-amino-3,5-dichloro-6-fluoropyridin-2-yl)oxyacetate Chemical group CCCCCCC(C)OC(=O)COC1=NC(F)=C(Cl)C(N)=C1Cl OLZQTUCTGLHFTQ-UHFFFAOYSA-N 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 7
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- IVDRCZNHVGQBHZ-UHFFFAOYSA-N 2-butoxyethyl 2-(3,5,6-trichloropyridin-2-yl)oxyacetate Chemical compound CCCCOCCOC(=O)COC1=NC(Cl)=C(Cl)C=C1Cl IVDRCZNHVGQBHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000375 suspending agent Substances 0.000 claims description 5
- 239000005627 Triclopyr Substances 0.000 claims description 4
- 230000003054 hormonal effect Effects 0.000 claims description 4
- REEQLXCGVXDJSQ-UHFFFAOYSA-N trichlopyr Chemical compound OC(=O)COC1=NC(Cl)=C(Cl)C=C1Cl REEQLXCGVXDJSQ-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 125000003158 alcohol group Chemical group 0.000 claims description 3
- 239000011230 binding agent Substances 0.000 claims description 3
- 239000006184 cosolvent Substances 0.000 claims description 3
- 239000002270 dispersing agent Substances 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 150000002576 ketones Chemical class 0.000 claims description 3
- 239000004530 micro-emulsion Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 3
- 150000003839 salts Chemical group 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 239000003381 stabilizer Substances 0.000 claims description 3
- 239000004562 water dispersible granule Substances 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 239000000080 wetting agent Substances 0.000 claims description 3
- 239000005558 Fluroxypyr Substances 0.000 claims description 2
- 239000007900 aqueous suspension Substances 0.000 claims description 2
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 2
- 239000004495 emulsifiable concentrate Substances 0.000 claims description 2
- 239000012053 oil suspension Substances 0.000 claims description 2
- ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 abstract description 19
- 240000004659 Picrasma quassioides Species 0.000 abstract description 5
- 235000010913 Picrasma quassioides Nutrition 0.000 abstract description 5
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 description 11
- 239000000575 pesticide Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000000469 ethanolic extract Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000009331 sowing Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 238000012271 agricultural production Methods 0.000 description 3
- 238000001784 detoxification Methods 0.000 description 3
- MAGVJLLHDZWQFM-UHFFFAOYSA-N n-chloro-n-methylmethanamine Chemical class CN(C)Cl MAGVJLLHDZWQFM-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- ZKFARSBUEBZZJT-UHFFFAOYSA-N 1-butoxypropan-2-yl 2-(4-amino-3,5-dichloro-6-fluoropyridin-2-yl)oxyacetate Chemical group CCCCOCC(C)OC(=O)COC1=NC(F)=C(Cl)C(N)=C1Cl ZKFARSBUEBZZJT-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 125000002147 dimethylamino group Chemical class [H]C([H])([H])N(*)C([H])([H])[H] 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000005886 Chlorantraniliprole Substances 0.000 description 1
- 239000005499 Clomazone Substances 0.000 description 1
- NRFQZTCQAYEXEE-UHFFFAOYSA-N Fenclorim Chemical compound ClC1=CC(Cl)=NC(C=2C=CC=CC=2)=N1 NRFQZTCQAYEXEE-UHFFFAOYSA-N 0.000 description 1
- GRSMWKLPSNHDHA-UHFFFAOYSA-N Naphthalic anhydride Chemical compound C1=CC(C(=O)OC2=O)=C3C2=CC=CC3=C1 GRSMWKLPSNHDHA-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- PSOVNZZNOMJUBI-UHFFFAOYSA-N chlorantraniliprole Chemical compound CNC(=O)C1=CC(Cl)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl PSOVNZZNOMJUBI-UHFFFAOYSA-N 0.000 description 1
- KIEDNEWSYUYDSN-UHFFFAOYSA-N clomazone Chemical compound O=C1C(C)(C)CON1CC1=CC=CC=C1Cl KIEDNEWSYUYDSN-UHFFFAOYSA-N 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- MEFQWPUMEMWTJP-UHFFFAOYSA-N fluroxypyr Chemical compound NC1=C(Cl)C(F)=NC(OCC(O)=O)=C1Cl MEFQWPUMEMWTJP-UHFFFAOYSA-N 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses an application method of Picrasma quassioides (D.Don) Benn.) extract in relieving phytotoxicity of hormone herbicides on rice, which comprises the following steps: step 1: pulverizing ramulus Et folium Picrasmae, sieving with 40-100 mesh sieve, extracting with solvent, and concentrating to obtain paste extract; step 2: the paste extract and its composition can be used for crops. The quassia extract can enhance the tolerance of crops to herbicides to improve the selectivity of the herbicides, can be used for preparing herbicide safeners, can protect the crops from the toxicity of the herbicides to a certain extent, and can enhance the selectivity of the herbicides by enhancing the tolerance of the crops to the herbicides without influencing the sensitivity of target weeds to the herbicides.
Description
Technical Field
The invention relates to the technical field of natural medicinal chemistry and pesticides, in particular to an application method of a quassia extract in relieving phytotoxicity of hormone herbicides on rice.
Background
Chemical weeding is an important sign for realizing agricultural modernization. However, the herbicide is a 'double-edged sword', and improper use of the herbicide is very easy to cause phytotoxicity to crops. The most effective way to solve the phytotoxicity of herbicides is to use safeners. The safeners can expand the application range of the herbicide, once the corresponding safeners are found in the existing herbicide varieties with low selectivity, the safeners can solve the safety problem of the herbicide on some crops, and the herbicide can also become new varieties applied in the fields of the crops, thereby prolonging the service life and avoiding the huge cost of developing new herbicides. Therefore, the development of herbicide safeners is of great significance to agricultural production.
The safeners currently used are almost all from chemical synthesis, and the commercialized herbicide safeners comprise fenclorim, clomazone, chlorantraniliprole, fenchloranil, naphthalic anhydride and the like. The countries studied are mainly america, swiss, germany, japan, canada, russia, hungary, etc. China also pays much attention to the subject, but research on the synthesis and biological activity of the safeners is still in the beginning. Monomer substances with detoxification activity are extracted, separated and purified from plants, and chemical synthesis and structure optimization are carried out on the substances with detoxification activity, so that an important way is provided for development of safeners.
Chemical safeners inevitably pose new potential environmental pollution risks during synthesis and use. The plant source safener is derived from plants, has the main performance of a chemically synthesized safener and also has the environmental protection advantage which the chemically synthesized safener does not have. The development of biopesticides including plant-derived safeners in China is one of effective ways for reducing the dosage of chemical pesticides, and is also a basic requirement and an important guarantee for the development of modern green and organic agricultural production. Therefore, the plant source safener is bound to play an increasingly important role in the green agricultural production in the future, and has wide market demands in China and even the global scope.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to solve the problem that hormone herbicides have phytotoxicity on crops in the prior art, and provides an application method of a quassia extract in relieving the phytotoxicity of the hormone herbicides on rice.
2. Technical scheme
In order to achieve the purpose, the invention adopts the following technical scheme:
the application method of the quassia extract in relieving the phytotoxicity of hormone herbicides on rice comprises the following steps:
step 1: pulverizing ramulus Et folium Picrasmae, sieving with 40-100 mesh sieve, extracting with solvent, and concentrating to obtain paste extract;
step 2: the paste extract and its composition can be used for crops.
Preferably, the hormonal herbicide includes 2 m 4 chloro or its ester or salt forms such as sodium, potassium, dimethylamine salts, isooctyl ester and the like, fluroxypyridine esters, fluroxypyr-meptyl ester, fluroxypyr-2-butoxy-1-methylethyl ester, triclopyr, butoxyethyl triclopyr, and combinations thereof.
Preferably, the quassia extract is an alcohol, ketone or ester extract of quassia, and the alcohol comprises one or more of methanol, ethanol, propanol, butanol, pentanol, acetone and ethyl acetate.
The invention also provides a composition for relieving the phytotoxicity of the hormone herbicide on crops, which is a composition A prepared by mixing the quassia extract with one or more than one processing acceptable carrier or a composition B prepared by mixing the quassia extract with the hormone herbicide and one or more than one processing acceptable carrier.
Preferably, the weight percentage of the quassia extract in the composition is 0.1-99%.
Preferably, the processing-acceptable carrier is selected from one or more of an emulsifier, a dispersant, a wetting agent, a penetrant, a cosolvent, a stabilizer, a synergist and a binder.
Preferably, the composition can be prepared into missible oil, microemulsion, water suspending agent, aqueous emulsion, wettable powder, soluble granules, water dispersible granules, suspending agent, dispersible oil suspending agent and the like.
The invention also provides the application of the composition in reducing the phytotoxicity of the herbicide to crops, wherein the composition A is used for soaking the crops, or is applied to the crops or the soil to which the hormone herbicide is applied, or is applied to the crops or the soil after being combined with the hormone herbicide; or directly applying the composition B to crops or soil
Preferably, the quassia extract is used in an effective and safe amount of 1-4000 g/mu.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) According to the invention, the quassia extract can protect crops from being poisoned by herbicides to a certain extent, and the herbicide selectivity is improved by enhancing the tolerance of the crops to the herbicides without influencing the sensitivity of target weeds to the herbicides.
(2) In the invention, the research shows that the picrasma quassioides ethanol extract can reduce the concentration of the chloropyridyl ester to 1-72g a.i./hm to a certain extent under the appropriate concentration 2 The rice phytotoxicity of (1), wherein, the protection effect on rice seedlings is the best when the concentration of the ethanol extract of the quassia tree is 50g/L and 200g/L, and the plant height can reach 90.23 percent and 91.58 percent of the blank control.
Drawings
FIG. 1 is a comparison chart of the research on the protection effect of ethanol extract of quassia on the growth of rice seedlings, wherein ck, medicine + quassia are shown from left to right in the chart.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1:
an application method of the quassia extract in relieving phytotoxicity of hormone herbicides on rice comprises the following steps:
step 1: pulverizing ramulus Et folium Picrasmae, sieving with 40-100 mesh sieve, extracting with solvent, and concentrating to obtain paste extract with water content of 10-85%;
and 2, step: the pasty extract and the composition thereof are used for crops, and the mass percentage of the quassia extract in the composition is 0.1-99%.
The hormonal herbicides in this embodiment include 2-methyl-4-chloro or its ester or salt forms (e.g., sodium, potassium, dimethylamine salts, isooctyl ester, etc.), fluroxypyr-meptyl, fluroxypyr-2-butoxy-1-methylethyl ester, triclopyr, butoxyethyl ester of triclopyr, and combinations thereof.
In this embodiment, the quassia extract is an alcohol, ketone or ester extract of quassia, and the alcohol includes one or more of methanol, ethanol, propanol, butanol, pentanol, acetone and ethyl acetate.
In this embodiment, the composition for alleviating phytotoxicity of the hormone herbicide on the crops is a composition a prepared by mixing the quassia extract with one or more processing acceptable carriers or a composition B prepared by mixing the quassia extract with the hormone herbicide and one or more processing acceptable carriers.
In this embodiment, the processing-acceptable carrier is one or more selected from an emulsifier, a dispersant, a wetting agent, a penetrant, a cosolvent, a stabilizer, a synergist, and a binder.
In the embodiment, the composition can be prepared into missible oil, microemulsion, water suspension, emulsion in water, wettable powder, soluble granules, water dispersible granules, suspending agent, dispersible oil suspension and the like.
In the embodiment, the composition is applied to the aspect of reducing the phytotoxicity of the herbicide to crops, namely, the composition A is used for soaking the crops, or is applied to the crops or the soil to which the hormone herbicide is applied, or is applied to the crops or the soil after being combined with the hormone herbicide; or directly applying the composition B to crops or soil, wherein the effective safe use amount of the quassia extract is 1-4000 g/mu.
In this example, the quassia extract is pre-treated prior to use, comprising the steps of: dissolving 1-500 parts of ramulus Et folium Picrasmae extract in 1-500 parts of organic solvent such as ethyl acetate or ethanol, adding 10-50000 parts of water to obtain mixed solvent, and adding 0.02-5 parts of Tween-80 for dispersing.
In the embodiment, the first discovery shows that the damage of the picrasma quassioides extract obtained after drying, crushing, sieving, extracting by using alcohol substances and concentrating the extract to crops can play a role in protecting. The quassia extract and the composition thereof are applied to crops, can protect the crops from the phytotoxicity of hormone herbicides, and can be used for preparing herbicide safeners.
Example 2:
test results and analysis:
1.1 Experimental materials:
the test rice variety: huanghuazhan (Yuanyongping agricultural high-tech, ltd.)
The test plant material: cleaning the tested materials, drying, pulverizing, sealing, and freezing at-25 deg.C.
Main apparatus and equipment: ethanol, tween 20, distilled water, ethyl acetate, n-hexane, chloroform, acetone, etc. are analytically pure (national medicine group reagent Co., ltd.)
The test soil is rice matrix soil.
1.2 Experimental methods:
(1) Extracting effective components of plant materials: the shaking extraction method is adopted, the plant dry material is weighed in an electronic balance and placed in a conical flask, 95% ethanol is added, and the material is dissolved in the ethanol by stirring with a glass rod. Placing the mixture in a shaking table (the rotating speed is set to be 60 r) to shake and extract for 2h. And then, the plant material is placed in a funnel, a glass rod is used for drainage, and filter paper is used for filtration, so that the filtration speed is increased. Concentrating to paste by using a rotary evaporator, scraping the extract, washing the distillation flask with absolute ethyl alcohol for several times, transferring the extract and the washing liquid into a volumetric flask, and fixing the volume with the absolute ethyl alcohol. Preparing 50g/L of quassia absolute ethyl alcohol crude extract, and storing in a refrigerator at 4 ℃ for later use.
(2) And (3) culturing rice seedlings: selecting plump rice seeds with basically consistent size for germination accelerating and sowing, soaking the rice seeds in the picrasma quassioides ethanol extract at room temperature for 24h, sterilizing the soaked seeds with 3% sodium hypochlorite solution for about 20min, and cleaning with ultrapure water for 2-3 times. Two layers of filter paper are laid in a culture dish, the disinfected seeds are placed in the culture dish, the seeds are kept moist by ultrapure water, a preservative film with holes is used for sealing, the culture dish is placed in an artificial intelligent climate box with the temperature of 27 ℃ and the relative humidity of 85% for germination acceleration for 48 hours, and water is added during the germination acceleration to keep the rice seeds moist. Selecting rice seeds with consistent bud length from various varieties, sowing the rice seeds in square plastic boxes (with the length of 13cm, the width of 8cm and the height of 5 cm), filling 200g of soil which is sieved and air-dried and is not used with herbicide into each box, keeping the soil humidity at about 20%, uniformly sowing 30 rice seeds in each box, sowing each box, covering 0.5-1.0cm of fine soil on each box, placing the box in a greenhouse for cultivation, fixing 15 seedlings/box after rice seedlings emerge neatly, watering at regular time, and keeping moisture.
(3) Experiments of reducing phytotoxicity of quassia: the method comprises the steps of soaking seeds in 50g/L of picrasma quassioides ethanol extract, accelerating germination and sowing, performing stem leaf treatment by using a 3WP-2000 type walking spray tower developed by Nanjing mechanization research institute of agricultural department according to the recommended dosage of the field when the rice grows to 2-3 leaf stage, setting clear water for comparison, repeating three treatments for each treatment, and continuously culturing under the greenhouse condition after pesticide application. And 7d and 15d after the pesticide is applied, investigating the plant height of the rice seedlings, observing the growth condition of the rice, and calculating the plant height. Attached: selection of four pesticide dosages: 75 ml/mu of 20% fluroxypyr emulsifiable concentrate; 40% of 2 fluroxypyr meptyl oil 70 ml/mu; 2, 40 ml/mu of methyl 4 chlorodimethylamine salt aqueous solution; triclopyr butoxyethyl ester 30 ml/acre.
(4) Data processing: and recording the plant height data of each group, the average value of the plant height and the percentage of the plant height relative to CK by using EXCEL.
2 results and analysis:
in this example, as shown in Table 1, after applying only four kinds of agricultural chemicals (20% fluroxypyr meptyl, 40%2 m fluroxypyr meptyl, 2 m 4 chlorodimethylamine salt aqua and butoxyethyl triclopyr) for 15d, rice remarkably suffered phytotoxicity, and the plant height became remarkably short, accounting for only 53.61%,57.39%,69.07% and 49.83% of CK. The plant heights of the control group soaked by the quassia quassioides account for 96.91%,95.88%,87.97% and 50.17% compared with CK. Therefore, the seed soaking by the quassia tree extract can relieve the four pesticide hazards, particularly the first three pesticide hazards, and the butoxyethyl triclopyr has no obvious effect.
TABLE 1 detoxification effects of quassia extracts on rice phytotoxicity of four pesticides
In the present example, as shown in fig. 1, under the treatment of applying the pesticide alone, the rice seedlings were short, growth was inhibited, and symptoms such as yellowing and stem and leaf spreading occurred. These symptoms were recovered by adding ethanol extract of quassia tree. Moreover, the growth of rice seedlings, to which 20% fluroxypyr meptyl, 40%2 m fluroxypyr meptyl, 2 m 4 chlorodimethylamine salt aqueous solution was applied, was restored to the control level, and the effect of butoxyethyl triclopyr was insignificant.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (9)
1. An application method of the quassia extract in relieving phytotoxicity of hormone herbicides on rice is characterized by comprising the following steps:
step 1: pulverizing ramulus Et folium Picrasmae, sieving with 40-100 mesh sieve, extracting with solvent, and concentrating to obtain paste extract;
step 2: the paste extract and its composition can be used for crops.
2. The method of using quassia extract for reducing phytotoxicity of hormonal herbicides on rice as claimed in claim 1, wherein said hormonal herbicides include 2 m 4 chloro or its ester or salt form such as sodium, potassium, dimethylamine or isooctyl, fluroxypyridine ester, fluroxypyr meptyl, fluroxypyr-meptyl, 2-butoxy-1-methylethyl fluroxypyr, triclopyr, butoxyethyl triclopyr and combinations thereof.
3. The method for applying quassia extract to relieve the phytotoxicity of hormone herbicides on rice as claimed in any one of claims 1 and 2, wherein the quassia extract is alcohol, ketone or ester extract of quassia, and the alcohol comprises one or more of methanol, ethanol, propanol, butanol, pentanol, acetone and ethyl acetate.
4. The composition for relieving the phytotoxicity of the hormone herbicide on crops is a composition A prepared by mixing a quassia extract with one or more than one processing acceptable carrier or a composition B prepared by mixing the quassia extract with the hormone herbicide and one or more than one processing acceptable carrier.
5. The composition according to claim 4, wherein the quassia extract is present in the composition in an amount of 0.1 to 99% by weight.
6. The composition according to any one of claims 4 and 5, wherein the processing-acceptable carrier is selected from one or more of an emulsifier, a dispersant, a wetting agent, a penetrant, a cosolvent, a stabilizer, a synergist and a binder.
7. The composition of claim 6, wherein the composition can be prepared into emulsifiable concentrate, microemulsion, water suspension, aqueous emulsion, wettable powder, soluble granule, water dispersible granule, suspending agent, dispersible oil suspension and the like.
8. The use of the composition of claim 4 for reducing the phytotoxicity of herbicides on crops, wherein the composition A is used for seed soaking of crops, or applied to crops or soil to which the hormone herbicides have been applied, or applied to crops or soil after being combined with the hormone herbicides; or the composition B is directly applied to crops or soil.
9. The use according to claim 8, wherein the quassia extract is used in an effective and safe amount of 1-4000 g/acre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211618080.8A CN115918654A (en) | 2022-12-15 | 2022-12-15 | Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211618080.8A CN115918654A (en) | 2022-12-15 | 2022-12-15 | Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115918654A true CN115918654A (en) | 2023-04-07 |
Family
ID=86697480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211618080.8A Pending CN115918654A (en) | 2022-12-15 | 2022-12-15 | Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115918654A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823588A (en) * | 2012-09-12 | 2012-12-19 | 湖南农业大学 | Application of furan coumarin compound in reducing toxic effect of amide herbicide on crops |
| CN105519525A (en) * | 2016-03-01 | 2016-04-27 | 湖南农业大学 | Application of Cnidium monnieri (L.) Cuss extract in reducing drug misadventure of herbicide on crops |
| CN105660635A (en) * | 2016-03-01 | 2016-06-15 | 湖南农业大学 | Application of Angelica pubescens Maxim. f. Biserrata Shan et Yuan extract in reducing of phytotoxicity of herbicide to crops |
| CN106561646A (en) * | 2015-10-10 | 2017-04-19 | 重庆市涪陵区屋机花椒种植场 | A method for relieving poisoning effects of amide-type herbicides to rice by utilizing a zanthoxylum bungeanum extract product |
| CN109221263A (en) * | 2018-10-30 | 2019-01-18 | 湖南人文科技学院 | A kind of botanical herbicides safener and the method for alleviating rice drug misadventuring |
| CN110074116A (en) * | 2019-06-03 | 2019-08-02 | 江西鑫邦科技有限责任公司 | A kind of pyridines Herbicidal combinations of brassinolide-containing class compound |
| CN110074126A (en) * | 2019-05-31 | 2019-08-02 | 江西鑫邦科技有限责任公司 | A kind of phenoxy carboxylic acid Herbicidal combinations of brassinolide-containing class compound |
| CN114341142A (en) * | 2019-09-02 | 2022-04-12 | 先正达农作物保护股份公司 | Strigolactone derivatives as plant growth regulator compounds |
-
2022
- 2022-12-15 CN CN202211618080.8A patent/CN115918654A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823588A (en) * | 2012-09-12 | 2012-12-19 | 湖南农业大学 | Application of furan coumarin compound in reducing toxic effect of amide herbicide on crops |
| CN106561646A (en) * | 2015-10-10 | 2017-04-19 | 重庆市涪陵区屋机花椒种植场 | A method for relieving poisoning effects of amide-type herbicides to rice by utilizing a zanthoxylum bungeanum extract product |
| CN105519525A (en) * | 2016-03-01 | 2016-04-27 | 湖南农业大学 | Application of Cnidium monnieri (L.) Cuss extract in reducing drug misadventure of herbicide on crops |
| CN105660635A (en) * | 2016-03-01 | 2016-06-15 | 湖南农业大学 | Application of Angelica pubescens Maxim. f. Biserrata Shan et Yuan extract in reducing of phytotoxicity of herbicide to crops |
| CN109221263A (en) * | 2018-10-30 | 2019-01-18 | 湖南人文科技学院 | A kind of botanical herbicides safener and the method for alleviating rice drug misadventuring |
| CN110074126A (en) * | 2019-05-31 | 2019-08-02 | 江西鑫邦科技有限责任公司 | A kind of phenoxy carboxylic acid Herbicidal combinations of brassinolide-containing class compound |
| CN110074116A (en) * | 2019-06-03 | 2019-08-02 | 江西鑫邦科技有限责任公司 | A kind of pyridines Herbicidal combinations of brassinolide-containing class compound |
| CN114341142A (en) * | 2019-09-02 | 2022-04-12 | 先正达农作物保护股份公司 | Strigolactone derivatives as plant growth regulator compounds |
Non-Patent Citations (3)
| Title |
|---|
| 张秋裕 等: "苦木化学成分研究", 《中草药》, vol. 51, no. 19, pages 4884 - 4890 * |
| 王琦 等: "271 苦木科植物化学成分及生物活性研究进展", 《国外医学中医中药分册》, vol. 26, no. 5, pages 277 - 280 * |
| 赵文娜 等: "苦木化学成分和药理作用研究进展", 《中药材》, vol. 34, no. 7, pages 1149 - 1152 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| May et al. | An assessment of the allelopathic potential of Eucalyptus | |
| Xudan | The effect of foliar application of fulvic acid on water use, nutrient uptake and yield in wheat | |
| Liu et al. | Biologically active secondary metabolites of barley. II. Phytotoxicity of barley allelochemicals | |
| CN102273459A (en) | Weeding composition containing pyribenzoxim and cyhalofopbutyl | |
| CN111903715A (en) | Direct-seeding rice cold-resistant and flood-resistant seed coating agent and preparation method and use method thereof | |
| EP3003034A1 (en) | Natural bioherbicides and related materials and methods | |
| NAQVI | ITALIAN RYEGRAss (zozr zor L.) | |
| CN111018697B (en) | Method for extracting trans-p-hydroxycinnamic acid from corn straws and application of trans-p-hydroxycinnamic acid as herbicide | |
| CN106172481A (en) | A kind of plant seed developmental inhibitors for controlling weeds and application thereof | |
| CN115918654A (en) | Application method of quassia extract in relieving phytotoxicity of hormone herbicides on rice | |
| CN103988837A (en) | Garden nursery weed killer and use method thereof | |
| Gunaeni et al. | The Effect of Plant Growth Regulators and Planting Density against Viral Infection and the Production from Bulbs of True Shallot Seed in the Highlands | |
| CN105660635A (en) | Application of Angelica pubescens Maxim. f. Biserrata Shan et Yuan extract in reducing of phytotoxicity of herbicide to crops | |
| Bovey et al. | Occurrence of plant growth inhibitors in tropical and subtropical vegetation | |
| CN114163490A (en) | Iridoid compound and application thereof in preparation of herbicide | |
| CN106577666A (en) | Ternary combined weeding composition | |
| CN105519525A (en) | Application of Cnidium monnieri (L.) Cuss extract in reducing drug misadventure of herbicide on crops | |
| CN114052034B (en) | Herbicide and application thereof | |
| JP4428913B2 (en) | Plant germination inhibitor | |
| WO2002003803A1 (en) | Methods and compositions for modulating plant growth and senescence | |
| CN109497066A (en) | 15 β-the hydroxyklaineanone of antifeedant for insect of transporting in plant and application | |
| CN108552233A (en) | A kind of paraquat aqua and preparation method thereof | |
| CN110981730B (en) | Method for extracting trans-ethyl p-hydroxycinnamate from corn straws and application of trans-ethyl p-hydroxycinnamate as herbicide | |
| US5646091A (en) | Rehmannia glutinosa extract and a safener composition having safener activity to a herbicide paraquat | |
| CN111072483B (en) | Method for extracting methyl p-hydroxycinnamate from corn straws and application of methyl p-hydroxycinnamate as herbicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |