CN115886017A - Rat and insect killing composition and rat and insect killing method - Google Patents
Rat and insect killing composition and rat and insect killing method Download PDFInfo
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- CN115886017A CN115886017A CN202210696555.9A CN202210696555A CN115886017A CN 115886017 A CN115886017 A CN 115886017A CN 202210696555 A CN202210696555 A CN 202210696555A CN 115886017 A CN115886017 A CN 115886017A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a rat and insect killing composition and a method for killing rat and insect, wherein the rat and insect killing composition comprises a sterilant and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% and less than 0.1% by weight, based on the total weight of the rodenticidal composition. The method for killing the mouse insects comprises the step of feeding the mouse insect killing composition to the mice. The rodenticide killing composition has good palatability, and can control the population density and the quantity of parasites on the surface of the rats simultaneously.
Description
Technical Field
The invention relates to the field of hygiene of mouse and insect pest control, in particular to a mouse and insect killing composition and a method for killing mice and insects.
Background
Rat damage is one of the major biological disasters worldwide. The high population density of the rats not only causes great economic loss in the planting industry and the animal husbandry, but also can spread diseases, and seriously threatens the health and safety of human beings. Increased population density in rodents results in increased risk of developing murine disease epidemics. Plague is the most aggressive murine disease and one of the most serious infectious diseases that have historically occurred in humans. Acarids and fleas are important vector organisms of plague and other mouse-borne diseases and can transmit plague bacilli and other pathogens by absorbing blood among different individuals.
The traditional method for controlling the population density of the rats is to poison the rats by adopting a rodenticide so as to reduce the population density. However, the method has the problems of rapid rebound of the population density of the rats, great accidental injury to non-target animals, endangerment of animal welfare and the like. Moreover, after the traditional rodenticide kills the rats, fleas and the like parasitizing on the body surfaces of the rats leave the rats and are greatly overflowed in the environment. Fleas leaving the rat can survive in the environment for 1 month and then be further transferred to livestock or human beings, threatening human health. Therefore, there is a need for a rodenticide composition that can control both the population density and the number of ectoparasites in rodents.
In addition, the palatability of the rat and insect killing composition is closely related to the rat and insect killing effect, the palatability is better, the attraction to rats is stronger, the ingestion coefficient of the rats is higher, and the poisoning effect is better. Therefore, the selection of the rat and insect killing composition with better palatability has important significance for improving the rat and insect killing effect.
Therefore, a rat and insect killing composition which is good in palatability and can control the population density and the quantity of ectoparasites of rats is needed in practical work.
Disclosure of Invention
In view of the above, the present invention provides a rodenticide killing composition and a method for killing rodenticides by using the rodenticide killing composition, wherein the rodenticide killing composition has good palatability and can simultaneously control the population density and the number of ectoparasites of rats.
In view of the above objects, a first aspect of the present invention provides a rat/insect killing composition comprising: infertility agents and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% and less than 0.1% by weight, based on the total weight of the rodenticidal composition.
In the context of this specification, a "sterilant" refers to an agent which is capable of rendering a rat incapable of reproduction, with the goal of reducing the birth rate of the rat, and includes rendering a male rat incapable of producing sperm or incapable of producing healthy sperm; an agent which prevents a female mouse from producing a healthy egg, prevents fertilization of an egg, prevents implantation of a fertilized egg in the uterus, causes embryonic death, or the like.
In a preferred embodiment of the invention, the infertility agent is selected from at least one of levonorgestrel and ethinylestradiol, more preferably a combination of levonorgestrel and ethinylestradiol.
In a preferred embodiment of the invention, the amount of ivermectin is present in a content of greater than or equal to 0.0005% by weight and less than or equal to 0.05% by weight, based on the total weight of the rodenticidal insecticidal composition.
In a preferred embodiment of the present invention, the amount of ivermectin is 0.01% by weight based on the total weight of the rodenticidal disinfecting composition.
In a preferred embodiment of the invention, the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1.
In a preferred embodiment of the invention, the weight ratio of levonorgestrel, ethinylestradiol and ivermectin is 1.
In a second aspect of the present invention, there is provided a method of killing rodents comprising feeding the rodenticide killing composition as defined above to rodents.
Drawings
Fig. 1 shows the change in food intake within 7 days of the rat insect killer bait of the present invention, wherein a is the change in food intake of male big hamsters; b is the change of food intake of female big hamster; the low dose group diet contained 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin; the high dose group diet contained 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.1% ivermectin; # denotes p <0.05 in the low dose group compared to the control group; * Indicating p <0.05 in the high dose group compared to the control group.
FIG. 2 is a graph showing the change of male reproductive organs after the bait for mouse and insect killer EP-1 of the present invention is used for 7 days and then normally raised for 7 days, wherein A is a photograph showing the morphological changes of testis and epididymis of large hamster; b is the weight change of the testae of the big hamster; c is the weight change of the epididymis of the big hamsters; d is the weight change of the seminal vesicle of the big hamster; EP-1 bait contains 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin; * Indicating p <0.05 compared to control.
FIG. 3 is a graph showing the change of female reproductive organs after the rat and insect killer EP-1 bait is used for 7 days and then normally raised for 7 days, wherein A is the change of the weight of the ovary of a rat and an insect killer; b is the change of the weight of the uterus of the rat; EP-1 bait contains 0.005% of levonorgestrel, 0.005% of ethinyloestradiol and 0.01% of ivermectin; * Indicating p <0.05 compared to control.
FIG. 4 is a graph showing the behavior of parasitic ticks (Haemaphysalis longicornis) on the body surface of mice after 5 days of use of the mouse worm biocide EP-1 bait of the present invention, wherein A is the change in survival rate; b is the change in the blood satiety rate; EP-1 bait contains 0.005% of levonorgestrel, 0.005% of ethinyloestradiol and 0.01% of ivermectin; * Indicating p <0.05 compared to control.
FIG. 5 is a graph showing the survival rate of body surface parasitic fleas (Xenopsyllabeops) after treatment with the present rodenticide EP-1 bait, wherein the EP-1 bait contains 0.005% levonorgestrel, 0.005% ethinyloestradiol and 0.01% ivermectin; * Indicating p <0.05 compared to control.
FIG. 6 is a comparison of the average number of fleas on the surface of rats 7 days after the administration of the rat insect killer EP-1 bait of the present invention in Beijing Donglingshan area, wherein the EP-1 bait contains 0.005% levonorgestrel, 0.005% ethinylestradiol ether and 0.01% ivermectin; * Indicating p <0.05 compared to control.
Detailed Description
It should be noted that technical terms or scientific terms used in the embodiments of the present specification should have a general meaning as understood by those skilled in the art, unless otherwise defined.
The experimental procedures in the following examples are conventional unless otherwise specified. The raw materials and reagents used in the following examples are all commercially available products unless otherwise specified.
As mentioned in the background of the present specification, there is a need for a composition for killing and killing mouse parasites, which is palatable and can control both the population density of mice and the number of ectoparasites. It has been found that ivermectin is a highly effective broad-spectrum antiparasitic agent with good and rapid killing effect against a variety of parasites (e.g., mites, ticks, lice, fleas, fly larvae, etc.) and low toxicity to mammals and birds. Ivermectin has selective inhibitory effect, and binds to chloride ion channel with high affinity of invertebrate nerve cell and muscle cell with glutamic acid as valve, resulting in increased permeability of cell membrane to chloride ion, causing hyperpolarization of nerve cell or muscle cell, and paralysis or death of parasite. Therefore, the ivermectin can be added into the basic bait to prepare bait for feeding the rats, so that the aim of quickly killing various parasites on the surfaces of the rats is fulfilled.
However, prior art studies have shown that poor palatability of ivermectin added to base baits, such as prior art literature (J.Jacob, K.Aplin, D.M.Watson, L.A.Hinds; assassinging the efficacy of organic intakes of antibiotics on mobility of flies and ticks on commercial rats; journal of Pest Science (2021) 94. In this test, four types of granules containing different concentrations of ivermectin (2.3 mg/kg, 4.0mg/kg, 7.6mg/kg and 29.9mg/kg of ivermectin, respectively) were used to feed rats. Test results show that the ivermectin bait fed by the method can kill fleas on the body surface of a mouse, but the palatability of the granules is obviously deteriorated along with the increase of the concentration of the ivermectin in the granules, and particularly, the rat refuses the granules to a significant degree under the condition of high concentration of the ivermectin (the bait feeding amount of a control group is more than twice of that of an ivermectin group when the concentration of the ivermectin in the granules is 29.9mg/kg of the feed). And this prior art document explicitly states: the use of high concentrations of ivermectin should be avoided. However, in practical application, ivermectin needs to reach a certain concentration in bait to achieve the effect of killing various parasites on the surfaces of rats, and how to improve the palatability of the bait containing the high-concentration ivermectin becomes especially important.
The palatability refers to the food preference or acceptance degree of the rats to the rat and insect killing composition, and is an important parameter for measuring the performance of the rat and insect killing composition. The rat and insect killing effect of the rat and insect killing composition is closely related to the palatability of the composition, because: the palatability is good, the attraction to the rats is strong, the ingestion coefficient of the rats is high, and the poisoning effect is good; the palatability is poor, the ingestion coefficient of the rats is low, and the poisoning effect is poor. Therefore, the improvement of the palatability of the bait has important significance for improving the deratization effect.
The inventor finds that the combination of ivermectin and the sterilizing agent has good palatability, and can control the population density and the number of ectoparasites of the rats.
The invention adopts the combined use of the sterilant and the ivermectin to prepare the rat and insect killing composition, and achieves the effect of simultaneously reducing the population density of rats and the number of parasites on body surfaces. The raticide composition contains a sterilant and ivermectin which is an effective component for killing acarids and fleas, can continuously reduce the population density of the rats, can kill acarids and fleas in a short time, and can obviously reduce the occurrence risk of plague and other mouse-borne diseases.
The invention provides a method for detecting the appropriate content of ivermectin in the rat/insect killing composition. Determining that the amount of ivermectin is present in an amount greater than or equal to 0.0001 wt% and less than 0.1 wt%, based on the total weight of the rodenticidal composition; preferably, the amount of ivermectin is present in a content of greater than or equal to 0.0005% by weight and less than or equal to 0.05% by weight, based on the total weight of the rodenticidal insecticidal composition; more preferably, the amount of ivermectin is 0.01% by weight based on the total weight of the rodenticidal disinfecting composition.
The preferred infertility agent of the present invention is selected from at least one of levonorgestrel and ethinylestradiol, and more preferably a combination of levonorgestrel and ethinylestradiol.
In the preferable scheme, the optimal contents of the levonorgestrel and the ethinyloestradiol in the rat insect killing composition are searched, and the preferable weight ratio of the levonorgestrel to the ethinyloestradiol to the ivermectin is 1-1; most preferably, the weight ratio of levonorgestrel, ethinyloestradiol and ivermectin is 1.
The rodenticide killing composition of the present invention may further comprise a base bait, such as a commercially available large mouse growth feed, gramineous crop seeds, leguminous seeds, and bran-based baits, and the like.
The rodenticide insecticidal composition of the present invention can be prepared by a method conventional in the art, for example, by adding a sterilizing agent and ivermectin to the above-mentioned base bait to prepare a bait. The corresponding preparation method can be selected by the person skilled in the art according to the needs, and the preparation can also be carried out by adopting the method given in the embodiment of the invention.
The effects of the rat and insect killing composition are mainly reflected in three aspects of palatability, sterile effect and effect of killing ticks and fleas.
(1) Palatability
Example 2 of the present invention an experiment on the palatability of the rodenticide composition of the present invention was carried out. The baits used in this experiment were: a low dose group diet comprising 0.005% levonorgestrel, 0.005% ethinylestradiol ether, and 0.01% ivermectin; a high dose group diet containing 0.05% levonorgestrel, 0.05% ethinylestradiol ether, and 0.1% ivermectin. Palatability experiments prove that the ivermectin can cause severe food refusal of rats when the concentration of the ivermectin in the bait is 0.1%. When the concentration of the ivermectin in the bait is 0.01 percent, the influence on the palatability of the bait is limited, wherein the palatability of males has no obvious difference from a control 7 days after the bait is given; the palatability of females was not significantly different from the control at all time periods tested. This shows that the bait has better palatability when the dose of ivermectin is 0.01% or less when the sterilizing agent and the ivermectin are combined for use.
(2) Sterility Effect
Example 3 of the present invention was conducted to test the sterility effect of the rodenticide composition of the present invention. The baits used in this experiment were: EP-1 bait, which contains 0.005% levonorgestrel, 0.005% ethinyloestradiol and 0.01% ivermectin. The sterility experiment proves that when the male adult large hamster rat insecticide EP-1 bait and the female adult large hamster rat insecticide are respectively fed with the bait, the testis, epididymis and seminal vesicle weight of the male adult large hamster are remarkably reduced relative to a control (the male adult large hamster is fed with a commercial large mouse growth feed in which levonorgestrel, ethinylestradiol and ivermectin are not added), the ovary weight of the female adult large hamster is remarkably reduced relative to the control, the uterus weight is remarkably increased relative to the control, and the increase of the uterus weight can cause male sterility. This demonstrates that the use of a combination of a sterilant with ivermectin according to the invention results in sterility in rats.
(3) Effective in killing tick and flea
Examples 4 to 5 of the present invention conducted experiments on the killing effect of the rat insect-killing agent composition of the present invention on ticks and fleas indoors, and example 6 of the present invention conducted experiments on the killing effect of the rat insect-killing agent composition of the present invention on fleas outdoors. The baits used in this experiment were all EP-1 baits containing 0.005% levonorgestrel, 0.005% ethinylestradiol ether and 0.01% ivermectin. The indoor experiments on the killing effect of ticks and fleas prove that the death rate of the haemaphysalis parasitism on the body surface of the mouse can be 100 percent when the bait is fed with the mouse rat insect killer EP-1, and no saturated haemaphysalis individuals are found; this resulted in only 5.1% survival of Haematococcus on mice, which was much lower than 93.5% of the control (fed a commercial large mouse growth diet without levonorgestrel, ethinylestradiol and ivermectin added to it). The killing effect experiment of the wild rat on fleas proves that the flea number of the wild rat watchband is obviously reduced when the wild rat is fed with the bait EP-1 for killing the wild rat. This demonstrates that the use of the present invention in combination with ivermectin significantly reduces the mortality, satiety, survival and numbers of fleas and ticks parasitic on murine surfaces.
The present invention also provides a method of killing rodents comprising feeding the above rodent killing composition to rodents for controlling the number of murine and murine ectoparasites, including fleas (Siphonaptera), acarids (arachneidea).
The invention provides a feasible method for simultaneously controlling the population density of the mice and the number of the ectozoa of the mice, which is beneficial to preventing the mice from spreading diseases caused by the massive exozoa overflowing during large-scale rat killing.
The technical solution provided by the present invention is further described with reference to specific embodiments. The following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.
Levonorgestrel and ethinylene used in the examples below were purchased from Dalian Meiren Biotech, inc., ivermectin was purchased from sublimation Baike Biotech, zhejiang, and commercial rat and rat growth feeds were purchased from Australian cooperative feeds, beijing, and adult rat hamsters and ICR strain male mice were provided by the animal institute of Chinese academy of sciences.
Example 1 preparation of rat/insect killer bait
The rat and insect killer bait of the embodiment comprises a low-dose group bait and a high-dose group bait, wherein the low-dose group bait comprises: the mass ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1; high dose group baits: the mass ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1. The rat insect killer powder of the low-dose group (levonorgestrel, ethinyloestradiol and ivermectin mixed according to the mass ratio of 1. The ethanol solution was added to the clear water at a ratio of 1.
Using commercial rat and mouse growth feed as basic bait, gradually dripping the suspension liquid medicine into feed particles, uniformly mixing until the suspension liquid medicine is completely absorbed, and averagely 20-50ml of suspension liquid is mixed per 500g of mixture. And placing the mixed baits in a 45 ℃ oven to be dried for 12h in a dark place to prepare low-dose group baits and high-dose group baits. The bait can be preserved for about 1 month in a way of avoiding high temperature, humidity and light.
Example 2 palatability of rat and insect killer bait
This example used the low dose group baits and the high dose group baits prepared in example 1. The control diet did not contain the rat insect killer powder (only commercial large mouse growth feed) and the other operations were the same. The experiment adopts 12 adult large hamster males and females with the same weight, wherein the average male is about 130-135g, and the average female is about 125-130g. Experiments were performed in 2 x 2 groups of 6 per group, following the large hamster gender and high and low dose group bait treatment. All rats were housed individually in a single cage. 12h day and night. Each large hamster was fed 12g of bait daily for 7 days. And checking the death rate at intervals of 24h, collecting the residual baits, weighing, and calculating the bait consumption for 24 h.
The high dose group of male big hamsters consumed significantly less bait than the control (fig. 1A and 1B), which all consumed about 8-10g daily, while the high dose group was only 1-4g (fig. 1A). The feeding condition of the low-dose rat/insect killer bait is improved, and on the 7 th day of bait treatment, the feeding of the low-dose group is more than 5g, which has no significant difference with a control (figure 1A), and the influence of the low-dose ivermectin on the palatability of the bait is limited.
The palatability of the rat insect killer bait in female large hamsters was higher than that of males (fig. 1B). Control rats fed about 7-9g daily, while the high dose group was 1-6g (FIG. 1B). No significant change in palatability was found in the low dose group compared to the control, although bait consumption was lower in the low dose group than in the control at all time points (fig. 1B).
Example 3 detection of the sterility Effect of the rat/insect killer bait
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin), and the EP-1 bait was prepared as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat growth feed), and the other operations were the same. The experiment adopts 12 adult large hamster males and females with the same weight, wherein the average male is about 130-135g, and the average female is about 125-130g. Experiments were performed in 2 x 2 groups of 6 per group according to the sex of large hamsters and treatment with sterilant. All rats were housed individually in a single cage. 12h day and night. Each large hamster was fed 12g of bait daily for 7 days. After the fed rats are normally raised for 1 week, the genital organ changes including the weight of the testicle, the weight of the epididymis, the weight of the seminal vesicle, the weight of the ovary and the weight of the uterus are observed in an anatomical mode.
The male sterility effect of the EP-1 bait for killing rat and insect is shown in figure 2. The rodenticide bait had a significant effect on the weight of the reproductive organs of male hamsters (fig. 2A). After 7 days of bait treatment and then 7 days of normal feeding, the treated group had a significant drop in testicular weight, only about 1/3 of the control (2.7 g vs 0.8g, fig. 2B). Epididymis was similar to testis, and the weight of the treated group was even lower than 0.25g, which is much lower than 1g of the control group (FIG. 2C). The change in seminal vesicles was more pronounced, decreasing from an average of 2g in the control group to 0.3g in the treated group (fig. 2D).
The treatment with the feed EP-1 serving as the rodenticide has a significant effect on the weight of the reproductive organ of the female large hamster. The ovaries of the treated rats were significantly reduced to about 1/3 of the control (FIG. 3A). Uterine weight was significantly increased after treatment, approximately 1.8-fold higher than control (0.32 g vs. 0.58g, fig. 3B). The magnitude of the increase in uterine weight may lead to female infertility.
Example 4 the killing effect of a rat insect repellent bait on ticks indoors
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin), and the EP-1 bait was prepared as shown in example 1. The control diet did not contain the rat insect killer powder (only commercial large mouse growth feed) and the other operations were the same. Male mice of the ICR strain were used for the experiment at 10 weeks size, 4 per group. The experimental tick is haemaphysalis longicornis (haemaphysalis longicornis). At the beginning of the experiment, mice were fixed and fed with control and EP-1 bait, respectively, while ticks were placed on the body of the mice, 20-30 ticks per mouse. The survival rate and the blood saturation rate of the ticks are observed every 24h, the dropped ticks (whether because of blood saturation or death) are removed, the observation lasts for 5 days, and the cumulative survival rate and the blood saturation rate of the ticks for 5 days are calculated.
The bait treatment of the rat insect killer EP-1 has a very obvious and obvious effect on the survival of superficial ticks (haemaphysalis longicornis). Treatment of mice with EP-1 bait resulted in death of the Haematococcus longicornus which parasitized on the body surface, with a mortality rate of 100% within 5 days. No killed haemaphysalis individuals were found within 5 days of the control group (fig. 4A). The blood saturation rate of the haemaphysalis longicornis also fundamentally reversed within 5 days, and the blood saturation rate of the control group within 5 days is 100% when no haemaphysalis longicornis individuals are found in the treatment group (fig. 4B).
Example 5 the killing effect of a rat insect killer bait on fleas indoors
This example was conducted using rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin), and the bait was prepared in the low dose group as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat growth feed), and the other operations were the same. Male mice of the ICR strain were used in 10-week size, 3 mice per group. The experimental flea was Xenopsylla cheopis (Xenopsylla cheopis). Mice were first fed with control and EP-1 bait. After feeding for at least 3 days, the mice were fixed and the Trichoplusia repens were placed directly on the body of the mice, and approximately 100 Trichoplusia repens per mouse were placed to suck the blood for 3 hours. Afterwards, the Xenopus niveus attached to the surface of the mouse was carefully removed and placed in a clean 15ml centrifuge tube for feeding. After 48h, the survival rate of the Haemophilus impatiens is observed and recorded.
The treatment with the EP-1 bait as the raticide has a remarkable effect on the survival of body surface fleas (Xenopsylla cheopis). Treatment of mice with EP-1 bait feed resulted in death of Trichoplusia species (mice were fixed with an instrument, fleas imbibed for 3 hours) parasitizing the body surface. After the Haphularia impatiens collected on the body surface is normally raised for 48 hours, only 5.1 percent of the EP-1 bait treatment group survives; the average survival rate of the control group was as high as 93.5% (fig. 5).
Example 6 killing Effect of a rat insect killer bait on fleas in the wild
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin), and the EP-1 bait was prepared as shown in example 1. The control diet did not contain the rat insect killer powder (only commercial large mouse growth feed) and the other operations were the same. The experimental sample is arranged at the road side of the pear garden ridge-brickkiln line. Selecting an area with higher mouse density, setting a control area and an experimental area which are about 2 hectares, and adopting a grid type dosing method of throwing 10-15g of EP-1 bait every 5-6m to throw about 3kg of control and the EP-1 bait of the rat and insect killer respectively. 1 week after bait casting, 3 squares of 0.5 hectare size were selected in each area, and about 30-35 squirrel cages were caught for 3 days under each square. And counting the species, the weight and the sex of the captured mice, and detecting fleas on the body surface for counting.
The bait of the rodenticide EP-1 can reduce the number of fleas on the watchband of the wild rodent. The control group has the average flea number per mouse which is far higher than that of the experimental group (2.8 relative to 0.25, figure 6) and has a remarkable difference in flea number when the sample plot is selected and the bait is put in one time in the Beijing Donglingshan area and the detection is carried out after 7 days.
Claims (8)
1. A rodenticide killing composition comprising: infertility agents and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% by weight and less than 0.1% by weight, based on the total weight of the rodenticidal composition.
2. The rodenticidal composition according to claim 1, wherein the sterilant is at least one member selected from the group consisting of levonorgestrel and ethinyl.
3. The rodenticidal composition according to claim 1 or 2, wherein the amount of ivermectin is present in an amount of greater than or equal to 0.0005 wt% and less than or equal to 0.05 wt% based on the total weight of the rodenticidal composition.
4. The rodent-killing composition of claim 3, wherein the amount of ivermectin is 0.01 weight percent based on the total weight of the rodent-killing composition.
5. The rodenticidal composition according to claim 2, wherein the sterilant is a combination of levonorgestrel and ethinylestradiol.
6. The rodenticide insecticidal composition according to claim 5, wherein the weight ratio of levonorgestrel to ethinylestradiol to ivermectin is 1.
7. The rodenticide insecticidal composition according to claim 6, wherein the weight ratio of levonorgestrel, ethinylestradiol and ivermectin is 1.
8. A method of killing rodents comprising feeding the rodenticidal killing composition according to any one of claims 1 to 7 to rodents.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030040494A1 (en) * | 2001-07-23 | 2003-02-27 | Strobel Michael A. | Application of water and organic solvent soluble invermectin for topical and oral use |
| US20030215481A1 (en) * | 2002-05-09 | 2003-11-20 | Borchert Jeff N. | Control of ticks and fleas of rodents with systemic insecticides and insect growth regulators |
| CN1500387A (en) * | 2002-11-13 | 2004-06-02 | 中国科学院动物研究所 | Bait for controlling the fertility number of rats and the use thereof |
| US20150216888A1 (en) * | 2014-02-04 | 2015-08-06 | Iowa State University Research Foundation, Inc. | Antiparasitic polyanhydride nanoparticles |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869113B (en) * | 2010-06-17 | 2013-09-04 | 中国农业科学院植物保护研究所 | Cyclodextrin inclusion compound of rodents sterilant and preparation method thereof |
| CN103784484B (en) * | 2014-01-24 | 2016-05-11 | 河南科技大学 | A kind of castrating liquid and preparation method thereof for dog |
| CN103783052B (en) * | 2014-01-24 | 2015-12-02 | 河南科技大学 | A kind of novel mouse sterile bait and preparation method thereof |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030040494A1 (en) * | 2001-07-23 | 2003-02-27 | Strobel Michael A. | Application of water and organic solvent soluble invermectin for topical and oral use |
| US20030215481A1 (en) * | 2002-05-09 | 2003-11-20 | Borchert Jeff N. | Control of ticks and fleas of rodents with systemic insecticides and insect growth regulators |
| CN1500387A (en) * | 2002-11-13 | 2004-06-02 | 中国科学院动物研究所 | Bait for controlling the fertility number of rats and the use thereof |
| US20150216888A1 (en) * | 2014-02-04 | 2015-08-06 | Iowa State University Research Foundation, Inc. | Antiparasitic polyanhydride nanoparticles |
Non-Patent Citations (7)
| Title |
|---|
| J. JACOB 等: "Assessing the efficacy of oral intake of insecticides on mortality of fleas and ticks on commensal Rattus species", JOURNAL OF PEST SCIENCE, no. 94, pages 1543 * |
| 姜晓文;张春燕;于文会;冯国峰;徐利;: "复方伊维菌素乳液的毒性试验研究", 东北农业大学学报, no. 09, pages 101 - 105 * |
| 张知彬;: "左炔诺孕酮和炔雌醚复合物(EP-1)及组分对鼠类不育效果的研究进展", 兽类学报, no. 02, pages 203 - 210 * |
| 曲平;景海江;仇志华;: "应用伊维菌素驱杀实验动物体内外寄生虫", 实验动物科学, no. 01, pages 28 - 30 * |
| 翟青新;: "小鼠体外寄生虫的药浴治疗", 中国比较医学杂志, no. 02, pages 70 - 73 * |
| 邓林: "固定剂量复方阿苯达唑伊维菌素毒性研究", 中国优秀硕士学位论文全文数据库 农业科技辑, no. 1, pages 050 - 783 * |
| 陈千权;曲家鹏;刘明;张堰铭;: "高原鼠兔对炔雌醚、左炔诺孕酮和EP-1不育药饵适口性", 动物学杂志, no. 03, pages 87 - 90 * |
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