CN113545358A - Rat and insect killing composition and rat and insect killing method - Google Patents
Rat and insect killing composition and rat and insect killing method Download PDFInfo
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- CN113545358A CN113545358A CN202110770303.1A CN202110770303A CN113545358A CN 113545358 A CN113545358 A CN 113545358A CN 202110770303 A CN202110770303 A CN 202110770303A CN 113545358 A CN113545358 A CN 113545358A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a rat and insect killing composition and a method for killing rat and insect, wherein the rat and insect killing composition comprises a sterilant and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% and less than 0.1% by weight, based on the total weight of the rodenticidal composition. The method for killing the mouse insects comprises the step of feeding the mouse insect killing composition to the mice. The rodenticide killing composition has good palatability, and can control the population density and the quantity of parasites on the surface of the rats simultaneously.
Description
Technical Field
The invention relates to the field of hygiene of mouse and insect pest control, in particular to a mouse and insect killing composition and a method for killing mice and insects.
Background
The rat damage is one of the major natural disasters in the world. The high population density of the rats not only causes great economic loss in the planting industry and the animal husbandry, but also can spread diseases, and seriously threatens the health and safety of human beings. Increased population density in rodents results in increased risk of developing murine disease epidemics. Plague is the most aggressive murine disease and one of the most serious infectious diseases that have historically occurred in humans. Acarids and fleas are important vector organisms of plague and other mouse-borne diseases and can transmit plague bacilli and other pathogens by absorbing blood among different individuals.
The traditional method for controlling the population density of the rats is to poison the rats by adopting a rodenticide so as to reduce the population density. However, the method has the problems of rapid rebound of the population density of the rats, great accidental injury to non-target animals, animal welfare and the like. Moreover, the traditional rodenticide can cause fleas and the like parasitizing on the body surface of the rat to leave the rat body and largely spill into the environment after killing the rat. Fleas leaving the rat can survive in the environment for 1 month and further transfer to livestock or humans, threatening human health. Therefore, there is a need for a rodenticide composition that can control both the population density and the number of ectoparasites in rodents.
In addition, the palatability of the rat and insect killing composition is closely related to the rat and insect killing effect, the palatability is better, the attraction to rats is stronger, the ingestion coefficient of the rats is higher, and the poisoning effect is better. Therefore, the selection of the rodenticide killing composition with better palatability has important significance for improving the rodenticide killing effect.
Therefore, there is a need for a composition for killing and killing mouse insects, which is palatable and can control the population density and the number of ectoparasites of the mouse simultaneously.
Disclosure of Invention
In view of the above, the present invention provides a rodenticide killing composition and a method for killing rodenticides by using the rodenticide killing composition, wherein the rodenticide killing composition has good palatability and can simultaneously control the population density and the number of ectoparasites of rats.
In view of the above objects, the first aspect of the present invention provides a rodenticide composition comprising: infertility agents and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% and less than 0.1% by weight, based on the total weight of the rodenticidal composition.
In the context of this specification, a "sterilant" refers to an agent which is capable of rendering a rat incapable of reproduction, with the goal of reducing the birth rate of the rat, and includes rendering a male rat incapable of producing sperm or incapable of producing healthy sperm; an agent which prevents a female mouse from producing a healthy ovum or prevents fertilization of an ovum, or prevents a fertilized ovum from rooting in the uterus or causes embryonic death or deformity.
In a preferred embodiment of the invention, the infertility agent is selected from at least one of levonorgestrel and ethinylestradiol, more preferably a combination of levonorgestrel and ethinylestradiol.
In a preferred embodiment of the invention, the amount of ivermectin is present in a content of greater than or equal to 0.0005% by weight and less than or equal to 0.05% by weight, based on the total weight of the rodenticidal insecticidal composition.
In a preferred embodiment of the present invention, the amount of ivermectin is 0.01% by weight based on the total weight of the rodenticidal disinfecting composition.
In a preferred embodiment of the invention, the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1:1: 1-1: 1: 20.
In a preferred embodiment of the invention, the weight ratio of levonorgestrel, ethinylestradiol and ivermectin is 1:1: 2.
In a preferred embodiment of the present invention, the amount of levonorgestrel is 0.005 wt%, the amount of ethinylestradiol is 0.005 wt%, and the amount of ivermectin is 0.01 wt%, based on the total weight of the rat/insect-killing composition.
In a second aspect of the present invention, there is provided a method of killing rodents comprising feeding the rodenticide killing composition as defined above to rodents.
Drawings
Fig. 1 shows the change in food intake within 7 days of the rat insect killer bait of the present invention, wherein a is the change in food intake of male big hamsters; b is the change of food intake of female big hamster; the low dose group diet contained 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin; the high dose group diet contained 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.1% ivermectin; # denotes p <0.05 in the low dose group compared to the control group; denotes p <0.05 in the high dose group compared to the control group.
FIG. 2 is a graph showing the change of male reproductive organs after the bait for mouse and insect killer EP-1 of the present invention is used for 7 days and then normally raised for 7 days, wherein A is a photograph showing the morphological changes of testis and epididymis of large hamster; b is the weight change of the testae of the big hamster; c is the weight change of the epididymis of the big hamsters; d is the weight change of the seminal vesicle of the big hamster; EP-1 bait contains 0.005% of levonorgestrel, 0.005% of ethinyloestradiol and 0.01% of ivermectin; denotes p <0.05 compared to control.
FIG. 3 is a graph showing the change of female reproductive organs after the rat and insect killer EP-1 bait is used for 7 days and then normally raised for 7 days, wherein A is the change of the weight of the ovary of a rat and an insect killer; b is the change of the weight of the uterus of the rat; EP-1 bait contains 0.005% of levonorgestrel, 0.005% of ethinyloestradiol and 0.01% of ivermectin; denotes p <0.05 compared to control.
FIG. 4 is a graph showing the behavior of parasitic ticks (Haemaphysalis longicornis) on the body surface of mice after 5 days of use of the mouse worm biocide EP-1 bait of the present invention, wherein A is the change in survival rate; b is the change in the blood saturation rate; EP-1 bait contains 0.005% of levonorgestrel, 0.005% of ethinyloestradiol and 0.01% of ivermectin; denotes p <0.05 compared to control.
FIG. 5 is a graph showing the change in survival rate of body surface parasitic fleas (Xenopsylla cheopis) after treatment with an EP-1 bait according to the present invention, wherein the EP-1 bait contains 0.005% levonorgestrel, 0.005% ethinylestradiol ether, and 0.01% ivermectin; denotes p <0.05 compared to control.
FIG. 6 is a comparison of the average number of fleas on the surface of rats 7 days after the administration of the rat insect killer EP-1 bait of the present invention in Beijing Donglingshan area, wherein the EP-1 bait contains 0.005% levonorgestrel, 0.005% ethinylestradiol ether and 0.01% ivermectin; denotes p <0.05 compared to control.
Detailed Description
It should be noted that technical terms or scientific terms used in the embodiments of the present specification should have a general meaning as understood by those skilled in the art, unless otherwise defined.
The experimental procedures in the following examples are conventional unless otherwise specified. The raw materials and reagents used in the following examples are all commercially available products unless otherwise specified.
As mentioned in the background of the present specification, there is a need for a composition for killing and killing mouse pests, which is palatable and can control both the population density of the mouse and the number of ectoparasites. It has been found that ivermectin is a highly effective broad-spectrum antiparasitic agent with good and rapid killing effect against a variety of parasites (e.g., mites, ticks, lice, fleas, fly larvae, etc.) and low toxicity to mammals and birds. Ivermectin has selective inhibitory effect, and binds to chloride ion channel with high affinity of invertebrate nerve cell and muscle cell with glutamic acid as valve, resulting in increased permeability of cell membrane to chloride ion, causing hyperpolarization of nerve cell or muscle cell, and paralysis or death of parasite. Therefore, the ivermectin can be added into the basic bait to prepare bait for feeding the rats, so that the aim of quickly killing various parasites on the surfaces of the rats is fulfilled.
However, prior art studies have shown that the addition of ivermectin to base baits has poor palatability problems, for example, prior art documents (J.Jacob, K.Aplin, D.M.Watson, L.A.Hinds; Association of the efficacy of organic intakes of antibiotics on palatability of flies and clocks on commercial rats; Journal of Pest Science; https:// doi.org/10.1007/s 10340-020-. In this test, four types of granules containing different concentrations of ivermectin (2.3 mg/kg, 4.0mg/kg, 7.6mg/kg and 29.9mg/kg of ivermectin, respectively) were used to feed rats. The test results show that as the concentration of ivermectin in the granules increases, the palatability of the granules becomes significantly worse, the preference of the rats for the granules is significantly reduced (the preference is-2.5% at 7.6mg/kg feed), and particularly, the rat refusal to the granules is more significant at high concentration of ivermectin (the preference is-18% at 29.9mg/kg feed). And this prior art document explicitly states: the use of high concentrations of ivermectin should be avoided. However, in practical application, ivermectin needs to reach a certain concentration in bait to achieve the effect of killing various parasites on the surfaces of rats, and how to improve the palatability of the bait containing the high-concentration ivermectin becomes especially important.
The palatability refers to the food preference or acceptance degree of the rats to the rat and insect killing composition, and is an important parameter for measuring the performance of the rat and insect killing composition. The rat and insect killing effect of the rat and insect killing composition is closely related to the palatability of the rat and insect killing composition, because: the palatability is good, the attraction to the rats is strong, the ingestion coefficient of the rats is high, and the poisoning effect is good; the palatability is poor, the ingestion coefficient of the rats is low, and the poisoning effect is poor. Therefore, the improvement of the palatability of the bait has important significance for improving the deratization effect.
The inventor of the application finds that the combination of ivermectin and a sterilizing agent can improve the palatability of baits and control the population density and the number of ectozoa of rats.
The invention adopts the combined use of the sterilant and the ivermectin to prepare the rat and insect killing composition, and achieves the effect of simultaneously reducing the population density of rats and the number of parasites on body surfaces. The raticide composition contains a sterilant and ivermectin which is an effective component for killing acarids and fleas, can continuously reduce the population density of the rats, can kill acarids and fleas in a short time, and can obviously reduce the occurrence risk of plague and other mouse-borne diseases.
The invention searches the appropriate content of the ivermectin in the rat and insect killing composition. Determining that the amount of ivermectin is present in an amount greater than or equal to 0.0001 wt% and less than 0.1 wt%, based on the total weight of the rodenticidal composition; preferably, the amount of ivermectin is present in a content of greater than or equal to 0.0005% by weight and less than or equal to 0.05% by weight, based on the total weight of the rodenticidal insecticidal composition; more preferably, the amount of ivermectin is 0.01% by weight based on the total weight of the rodenticidal disinfecting composition.
The preferred infertility agent of the present invention is selected from at least one of levonorgestrel and ethinylestradiol, and more preferably a combination of levonorgestrel and ethinylestradiol.
In the preferable scheme, the optimal content of the levonorgestrel and the ethinylestradiol in the rat-worm killing composition is searched, and the preferable weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is finally determined to be 1:1: 1-1: 1: 20; preferably, the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1:1: 2; most preferably, the amount of levonorgestrel is 0.005 wt%, the amount of ethinylestradiol is 0.005 wt%, and the amount of ivermectin is 0.01 wt%, based on the total weight of the rodenticidal disinfecting composition.
The rodenticide killing composition of the present invention may further comprise a basal bait such as a commercially available large mouse growth feed, gramineous crop seeds, leguminous seeds, a bran-based bait, and the like.
The rodenticide insecticidal composition of the present invention can be prepared by a method conventional in the art, for example, by adding a sterilizing agent and ivermectin to the above-mentioned base bait to prepare a bait. The corresponding preparation method can be selected by the person skilled in the art according to the needs, and the preparation can also be carried out by adopting the method given in the embodiment of the invention.
The effects of the rat and insect killing composition are mainly reflected in three aspects of palatability, sterile effect and effect of killing ticks and fleas.
(1) Palatability
Example 2 of the present invention an experiment on the palatability of the rodenticide composition of the present invention was carried out. The baits used in this experiment were: a low dose group diet comprising 0.005% levonorgestrel, 0.005% ethinylestradiol ether, and 0.01% ivermectin (100 mg/kg); a high dose group diet comprising 0.005% levonorgestrel, 0.005% ethinylestradiol ether, and 0.1% ivermectin (1000 mg/kg). Palatability experiments prove that the mice can have severe food refusal when the consumption of the ivermectin is 1000mg/kg of bait, and the influence on the bait palatability is limited and has no obvious difference with a control when the consumption of the ivermectin is 100mg/kg of bait. The prior art literature demonstrates that food refusal in rats is very significant in the presence of high concentrations (29.9mg/kg feed) of ivermectin (see J.Jacob, K.Aplin, D.M.Watson, L.A.Hinds; Association of the efficacy of organic acids on mortalities of fats and ticks on commercial rats; Journal of Pest Science; https:/doi.org/10.1007/s 10340-020-. The result shows that the sterilizing agent and the ivermectin are combined for use, an unexpected technical effect is achieved in the aspect of improving the palatability, and the bait has better palatability when the consumption of the ivermectin is 100 mg/kg-1000 mg/kg.
(2) Sterility Effect
Example 3 of the present invention was conducted to test the sterility effect of the rodenticide composition of the present invention. The baits used in this experiment were: EP-1 bait, containing 0.005% levonorgestrel, 0.005% ethinyloestradiol and 0.01% ivermectin (100 mg/kg). The sterility experiment proves that when the male adult large hamster rat insecticide EP-1 bait and the female adult large hamster rat insecticide are respectively fed with the bait, the testis, epididymis and seminal vesicle weight of the male adult large hamster are remarkably reduced relative to a control (the male adult large hamster is fed with a commercial large mouse growth feed in which levonorgestrel, ethinylestradiol and ivermectin are not added), the ovary weight of the female adult large hamster is remarkably reduced relative to the control, the uterus weight is remarkably increased relative to the control, and the increase of the uterus weight can cause male sterility. This demonstrates that the use of a combination of a sterilant with ivermectin according to the invention results in sterility in rats.
(3) Effective in killing tick and flea
Examples 4 to 5 of the present invention conducted experiments on the killing effect of the rat insect-killing agent composition of the present invention on ticks and fleas indoors, and example 6 of the present invention conducted experiments on the killing effect of the rat insect-killing agent composition of the present invention on fleas outdoors. The baits used in this experiment were all EP-1 baits containing 0.005% levonorgestrel, 0.005% ethinylestradiol ether and 0.01% ivermectin (100 mg/kg). The indoor killing effect experiment on ticks and fleas proves that the death rate of the haemaphysalis longicornis parasitized on the body surface of the mouse can be 100% when the mouse worm killer EP-1 bait is fed, and no saturated haemaphysalis longicornis individual is found; results in only 5.1% survival of the fleas parasitizing the body surface of the mice, which is much lower than 93.5% survival of the controls (fed the commercial large mouse growth diet without levonorgestrel, ethinylestradiol and ivermectin added thereto). The killing effect experiment of the wild rat on fleas proves that the flea number of the wild rat watchband is obviously reduced when the wild rat is fed with the bait EP-1 for killing the wild rat. This demonstrates that the use of the present invention in combination with ivermectin significantly reduces the mortality, satiety, survival and numbers of fleas and ticks parasitic on murine surfaces.
The present invention also provides a method of killing rodents comprising feeding the above rodent killing composition to rodents for controlling the number of murine and murine ectoparasites, including fleas (Siphonaptera), acarids (arachneidea).
The invention provides a feasible method for simultaneously controlling the population density of the mice and the number of the ectozoa of the mice, which is beneficial to preventing the mice from spreading diseases caused by the massive exozoa overflowing during large-scale rat killing.
The technical solution provided by the present invention is further described with reference to specific embodiments. The following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.
Levonorgestrel and ethinylene used in the examples below were purchased from Dalian Meiren Biotech, Inc., ivermectin was purchased from sublimation Baike Biotech, Zhejiang, and commercial rat and rat growth feeds were purchased from Australian cooperative feeds, Beijing, and adult rat hamsters and ICR strain male mice were provided by the animal institute of Chinese academy of sciences.
Example 1 preparation of rat and insect killer bait
The rat and insect killer bait of the embodiment comprises a low-dose group bait and a high-dose group bait, wherein the low-dose group bait comprises: the mass ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1:1:2 (namely, the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is equivalent to that of a low-dose group bait containing 0.005% of levonorgestrel, 0.005% of ethinylestradiol and 0.01% of ivermectin (100mg/kg)), and the purity of each component is more than 99%; high dose group baits: the mass ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1:1:20 (namely, the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is equivalent to that of a high-dose group bait containing 0.005% of levonorgestrel, 0.005% of ethinylestradiol and 0.1% of ivermectin (1000mg/kg)), and the purity of each component is more than 99%. The rat insect disinfestation agent powder of the low-dose group (the levonorgestrel, the ethinyloestradiol and the ivermectin are mixed according to the mass ratio of 1:1: 2) and the rat insect disinfestation agent powder of the high-dose group (the levonorgestrel, the ethinyloestradiol and the ivermectin are mixed according to the mass ratio of 1:1: 20) are gradually stirred and dissolved in absolute ethyl alcohol at the temperature of 55 ℃. The total mass concentration is about 0.2 mg/ml. Adding the ethanol solution into clear water at a ratio of 1:10(v/v), and stirring to obtain a suspension liquid medicine.
The suspension liquid medicine is gradually dripped into feed particles by taking commercial rat and mouse growth feed as basic bait, and is uniformly mixed until the suspension liquid is completely absorbed, and 22ml of suspension liquid is formed in every 500g of mixture on average. And placing the mixed baits in a 45 ℃ oven to be dried for 12h in a dark place to prepare low-dose group baits and high-dose group baits. The bait can be preserved for about 1 month in a way of avoiding high temperature, humidity and light.
Example 2 palatability of rat and insect killer bait
This example used the low dose group baits and the high dose group baits prepared in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat and rat growth feed), and the other operations were the same. The experiment adopts 12 male and female adult hamsters with the same weight, wherein the average of male is about 130-. Experiments were performed in 2 x 2 groups of 6 per group, following the large hamster gender and high and low dose group bait treatment. All rats were housed individually in a single cage. The day and night time is 12h to 12 h. Each large hamster was fed 12g of bait daily for 7 days. And checking the death rate at intervals of 24h, collecting the residual baits, weighing, and calculating the bait consumption for 24 h.
The high dose group of male big hamsters consumed significantly less bait than the control (fig. 1A and 1B), which all consumed about 8-10g daily, while the high dose group was only 1-4g (fig. 1A). The feeding condition of the low-dose rat/insect killer bait is improved, and the feeding of the low-dose group is more than 5g on the 6 th and 7 th days of the bait treatment, so that the feeding is not obviously different from that of a control (figure 1A), and the influence of the low-dose ivermectin on the palatability of the bait is limited.
The palatability of the rat insect killer bait in female large hamsters was higher than that of males (fig. 1B). Control rats fed about 7-9g daily, while the high dose group was 1-6g (FIG. 1B). No significant change in palatability was found in the low dose group compared to the control, although bait consumption was lower in the low dose group than in the control at all time points (fig. 1B).
Example 3 detection of the sterility Effect of the rat/insect killer bait
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin (100mg/kg)), and the EP-1 bait was prepared as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat and rat growth feed), and the other operations were the same. The experiment adopts 12 male and female adult hamsters with the same weight, wherein the average of male is about 130-. Experiments were performed in 2 x 2 groups of 6 per group according to the sex of large hamsters and treatment with sterilant. All rats were housed individually in a single cage. The day and night time is 12h to 12 h. Each large hamster was fed 12g of bait daily for 7 days. After the fed rats are normally raised for 1 week, the genital organ changes including the weight of the testicle, the weight of the epididymis, the weight of the seminal vesicle, the weight of the ovary and the weight of the uterus are observed in an anatomical mode.
The male sterility effect of the EP-1 bait for killing rat and insect is shown in figure 2. The rodenticide bait had a significant effect on the weight of the reproductive organs of male hamsters (fig. 2A). After 7 days of bait treatment followed by 7 days of normal feeding, the treated group had a significant decrease in testicular weight, only about 1/3 in weight (2.7g versus 0.8g, fig. 2B) compared to the control group. Epididymis was similar to testis, and the weight of the treated group was even lower than 0.25g, which is much lower than 1g of the control group (FIG. 2C). The change in seminal vesicles was more pronounced, decreasing from an average of 2g in the control group to 0.3g in the treated group (fig. 2D).
The treatment with EP-1 bait as a rodenticide also had a significant effect on the weight of the reproductive organs of female large hamsters. The treated rats had a significant reduction in ovaries, at about 1/3 for the control (fig. 3A). Uterine weight was significantly increased after treatment, approximately 1.8-fold higher than control (0.32g vs. 0.58g, fig. 3B). The magnitude of the increase in uterine weight may lead to female infertility.
Example 4 the killing effect of a rat insect killer bait on ticks indoors
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin (100mg/kg)), and the EP-1 bait was prepared as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat and rat growth feed), and the other operations were the same. Male mice of the ICR strain were used for the experiment at 10 weeks size, 4 per group. The experimental tick is haemaphysalis longicornis (haemaphysalis longicornis). At the start of the experiment, mice were fixed and fed with control and EP-1 bait, respectively, while ticks were placed on the body of the mice, 20-30 ticks per mouse. The survival rate and the blood saturation rate of the ticks are observed every 24h, the dropped ticks (whether because of blood saturation or death) are removed, the observation lasts for 5 days, and the cumulative survival rate and the blood saturation rate of the ticks for 5 days are calculated.
The treatment of the feed EP-1 of the raticide has a very obvious and obvious effect on the survival of body surface ticks (haemaphysis iongicornis). Treatment of mice with EP-1 bait resulted in death of the surface-colonized haemaphysalis longicornis, with a mortality rate of 100% within 5 days. No killed haemaphysalis individuals were found within 5 days of the control group (fig. 4A). The blood saturation rate of the haemaphysalis longicornis also fundamentally reversed within 5 days, and the blood saturation rate of the control group within 5 days is 100% when no haemaphysalis longicornis individuals are found in the treatment group (fig. 4B).
Example 5 the killing effect of a rat insect killer bait on fleas indoors
This example was conducted using rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin (100mg/kg)) and the bait preparation method for the low dose group was as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat and rat growth feed), and the other operations were the same. Male mice of the ICR strain of 10 weeks size were used for the experiment, 3 mice per group. The experimental flea was Xenopsylla cheopis (Xenopsylla cheopis). Mice were first fed with control and EP-1 bait. After feeding for at least 3 days, the mice were fixed and the Trichoplusia repens were placed directly on the body of the mice, and approximately 100 Trichoplusia repens per mouse were placed to suck the blood for 3 hours. Thereafter, the Xenopus niveus attached to the surface of the mouse was carefully removed and placed in a clean 15ml centrifuge tube for feeding. After 48h, the survival rate of the Haemophilus impatiens is observed and recorded.
The treatment with the EP-1 bait as the raticide has a remarkable effect on the survival of body surface fleas (Xenopsylla cheopis). Treatment of mice with EP-1 bait feed resulted in death of Trichoplusia species parasitizing the body surface (mice were fixed with an appliance, fleas imbibed for 3 hours). After the Haphularia impatiens collected on the body surface is normally raised for 48 hours, only 5.1 percent of the EP-1 bait treatment group survives; the average survival rate of the control group was as high as 93.5% (fig. 5).
Example 6 killing Effect of a rat insect killer bait on fleas in the wild
This example was conducted using a rat insect killer EP-1 bait (containing 0.005% levonorgestrel, 0.005% ethinylestradiol and 0.01% ivermectin (100mg/kg)), and the EP-1 bait was prepared as shown in example 1. The control diet was not supplemented with this rat insect killer powder (only containing commercial rat and rat growth feed), and the other operations were the same. The experimental sample is arranged at the road side of the pear garden ridge-brickkiln line. Selecting an area with higher mouse density, setting a control area and an experimental area of about 2 hectares, and adopting a grid type dosing method of dosing 10-15g of EP-1 bait every 5-6m to dose about 3kg of control and the mouse and insect killer EP-1 bait in total. 1 week after bait casting, 3 squares of 0.5 hectare size were selected in each area, and about 30-35 squirrel cages were caught for 3 days under each square. And counting the species, the weight and the sex of the captured mice, and detecting fleas on the body surface for counting.
The bait of the rodenticide EP-1 can reduce the number of fleas on the watchband of the wild rodent. The control group has the average flea number per mouse which is far higher than that of the experimental group (2.8 relative to 0.25, figure 6) and has a remarkable difference in flea number when the sample plot is selected and the bait is put in one time in the Beijing Donglingshan area and the detection is carried out after 7 days.
Claims (9)
1. A rodenticide killing composition comprising: infertility agents and ivermectin; the amount of ivermectin is present in a content of greater than or equal to 0.0001% and less than 0.1% by weight, based on the total weight of the rodenticidal composition.
2. The rodenticidal composition according to claim 1, wherein the sterilant is at least one member selected from the group consisting of levonorgestrel and ethinyl.
3. The rodenticidal composition according to claim 1 or 2, wherein the amount of ivermectin is present in an amount of greater than or equal to 0.0005 wt% and less than or equal to 0.05 wt% based on the total weight of the rodenticidal composition.
4. The rodenticide killing composition according to claim 3, wherein the amount of ivermectin is 0.01% by weight based on the total weight of the rodenticide killing composition.
5. The rodenticidal composition according to claim 2, wherein the sterilant is a combination of levonorgestrel and ethinylestradiol.
6. The rodenticide killing composition according to claim 5, wherein the weight ratio of the levonorgestrel to the ethinylestradiol to the ivermectin is 1:1: 1-1: 1: 20.
7. The rodenticide insecticidal composition according to claim 6, wherein the weight ratio of levonorgestrel, ethinyloestradiol and ivermectin is 1:1: 2.
8. The rodenticide insecticidal composition according to claim 5, wherein the levonorgestrel is in an amount of 0.005 wt.%, the ethinylestradiol is in an amount of 0.005 wt.%, and the ivermectin is in an amount of 0.01 wt.%, based on the total weight of the rodenticide insecticidal composition.
9. A method of killing rodents comprising feeding the rodenticidal killing composition according to any one of claims 1 to 8 to rodents.
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| CN115088717A (en) * | 2022-05-10 | 2022-09-23 | 中国科学院动物研究所 | Rodenticide composition, rodenticide bait and method |
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