CN115886006A - Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight - Google Patents
Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight Download PDFInfo
- Publication number
- CN115886006A CN115886006A CN202211145990.9A CN202211145990A CN115886006A CN 115886006 A CN115886006 A CN 115886006A CN 202211145990 A CN202211145990 A CN 202211145990A CN 115886006 A CN115886006 A CN 115886006A
- Authority
- CN
- China
- Prior art keywords
- carvacrol
- fluazinam
- composition
- percent
- konjak
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to an agricultural bactericide composition, in particular to a bactericide composition containing fluazinam and carvacrol, wherein the mass ratio of the fluazinam to the carvacrol is 95-20. Compared with a single preparation, the sterilization composition has a remarkable synergistic effect on the konjac azalea disease, and the prevention effect is improved; the medicine consumption is reduced, the low toxicity and the low residue are realized, and the pollution to the environment is reduced; due to the difference of the antibacterial mechanisms of the two raw medicines, the generation of drug resistance is favorably slowed down, and meanwhile, the addition of the natural product is favorable for reducing the toxicity of the compound medicament. The invention has no obvious adverse effect on the leaves and the growth of the konjak, meets the safety requirement of pesticide preparations and has good prevention effect on the konjak filament disease.
Description
Technical Field
The invention relates to a pesticide emulsion in water and a pesticide wettable powder, in particular to a pesticide emulsion in water and a pesticide wettable powder compounded by fluazinam and carvacrol.
Background
Konjak is a perennial herb of the family of Araceae, is the only special commercial crop which can provide glucomannan in large quantity and is known at present, and is widely applied to the aspects of food, agriculture and medicine. Konjak is used as a key crop for industrialized development of Guizhou agriculture, and provides an important way for increasing the income of vast farmers. However, with the increase of the planting area and the growth period, the southern blight of konjak (Athlia rolfsii (GZCC 22-0087)) causes the yield of konjak to be reduced by 20-40%, and the healthy development of the konjak industry is seriously influenced.
Fluazinam is a light yellow crystal with the name fluazinam and the chemical formula C 13 H 4 Cl 2 F 6 N 4 O 4 Melting point 115 ℃, CAS No.: 79622-59-6, is a broad-spectrum high-effective protective bactericide. The structural formula is as follows:
carvacrol is a natural product extracted from thyme plant, having the chemical formula C 10 H 14 O, melting point 0 ℃, CAS No.: 499-75-2. Has the properties of being easily soluble in alcohol and ether, almost insoluble in water and capable of volatilizing with water vapor. The structural formula is as follows:
the frequent application of single fluazinam and carvacrol in the field can cause the rapid generation of drug resistance and reduce the control effect of the drug. Therefore, it is urgent to reduce the generation speed of drug resistance and increase the prevention effect of the drug in the field.
Disclosure of Invention
The invention aims to overcome the problems of insufficient control effect and easy generation of drug resistance of a single bactericide, and simultaneously, the toxicity of a compound medicament is reduced by adding a natural product, so that the bactericidal composition with better bactericidal effect, lower cost and slow generation of drug resistance is finally provided.
Fluazinam is a pyridylamine derivative and a dinitroaniline bactericide, and has broad-spectrum and efficient protection activity. Fluazinam inhibits all stages of the fungal infection process by inhibiting spore germination, hyphal break through, growth and sporulation. As a low-toxicity bactericide which has no cross resistance with other medicaments, the fluazinam can be used as foliar spray and can also be used for soil treatment, and has very good soil stability. Carvacrol is used as a plant source pesticide, and the antibacterial mechanism of the carvacrol mainly inhibits the germination of pathogenic bacteria spores or quickly penetrates cell membranes of pathogenic microorganisms through strong surface activity and fat solubility, so that the pathogenic microorganisms die due to water imbalance. The invention hopes to achieve the purpose of toxicity strength synergism by combining two agricultural medicines with different antibacterial mechanisms so as to achieve the purposes of reducing toxicity, improving antibacterial activity and delaying drug resistance.
The invention also aims to provide the bactericidal composition for preventing and controlling the konjac azalea disease.
In order to achieve the purpose, the invention provides a bactericidal composition, wherein the mass ratio of the bactericidal composition diniconazole to picoxystrobin is 95-20.
The bactericidal composition also comprises one or a mixture of more of a protective agent, a growth regulator, a plant nutrient or a soil regulator.
The invention also provides a bactericide, wherein the bactericide contains 1-20% of active ingredients of fluazinam and carvacrol; contains 0.1 to 50 percent of the composition and the balance of carriers and auxiliary agents acceptable to pesticides.
According to the preferred embodiment of the bactericide, the dosage forms are aqueous emulsion and wettable powder.
The invention also provides application of the bactericidal composition in preventing and treating the konjac southern blight.
The invention also provides a method for controlling crop diseases, which is to apply the bactericidal composition to the root, the rhizome or the leaf of the konjak before or after the konjak is infected with the diseases or to the soil where the konjak grows.
The technical solution of the present invention will be described in more detail below:
the invention provides a sterilization composition, which contains fluazinam and carvacrol, wherein the mass ratio of the fluazinam to the carvacrol is 95-20.
Preferably, the mass ratio of fluazinam to carvacrol is 95.
And mixing and dissolving the aqueous emulsion, 3% of agricultural emulsion 2201#, 2% of nonylphenol polyoxyethylene ether phosphate, 2% of agricultural emulsion 600# acid phospholipid, 10% of cyclohexanone and 20% of solvent oil S150 to obtain an oil phase. 5% ethylene glycol, 0.3% xanthan gum and deionized water are added to 100% to prepare a water phase. Adding the oil phase into the water phase, fully mixing, and performing high-shear emulsification to obtain the product.
For wettable powder, 4% calcium lignosulfonate, 4% NNO, 1% K-12, 5% white carbon black and light calcium are added to 100%, mixed uniformly and crushed to obtain the product.
All the "%" referred to in the present invention are "mass%" unless otherwise specified.
The bactericidal composition also comprises one or a mixture of more of a protective agent, a growth regulator, a plant nutrient or a soil regulator. The protectant, growth regulator, plant nutrient or soil regulator are commercially available products.
For the selection of the processing aid of the aqueous emulsion, the emulsifier is selected from the following components: nonylphenol polyoxyethylene (E0 = 10) ether phosphate ester, tristyrylphenol polyoxyethylene ether phosphate ester (Nongru 600# phosphate ester), nongru 700#, nongru 2201#, emulsifier T-60, TX-10, nongru 1601#, nongru 600#, nongru 400#; the solvent is selected from: xylene, toluene, cyclohexanone and solvent oil (trade name: S-150, S-180 and S-200).
The auxiliary agents for the wettable powder are as follows: the dispersant is selected from: polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates; the wetting agent is selected from: alkyl sulfates, alkyl sulfonates; the filler is selected from: diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder and the like.
The bactericidal composition can be provided in the form of a finished preparation, namely, all the substances in the composition are mixed; it can also be provided in a single dose formulation, mixed directly in a tub (tank) prior to use. The concentrates of the invention are typically mixed with water to give the desired concentration of active.
Compared with the prior art, the invention has the following beneficial effects:
(1) Fluazinam inhibits all stages of the fungal infection process by inhibiting spore germination, hyphal break through, growth and sporulation. Carvacrol is used as a plant source pesticide, and the antibacterial mechanism of the carvacrol mainly inhibits the germination of pathogenic bacteria spores or quickly penetrates cell membranes of pathogenic microorganisms through strong surface activity and fat solubility, so that the pathogenic microorganisms die due to water imbalance. Therefore, the antibacterial effect under the same mass concentration can be effectively improved by compounding medicaments with different antibacterial mechanisms.
(2) The two raw medicines forming the bactericidal composition have different antibacterial mechanisms, and are beneficial to delaying the generation of drug resistance of germs.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the invention to these embodiments. It will be appreciated by those skilled in the art that the present invention encompasses all alternatives, modifications and equivalents as may be included within the scope of the claims.
1. Preparation examples
The percentage contents in all the preparation ratios are mass percentages.
1. Aqueous emulsion
Example (b): 10% fluazinam carvacrol (effective ingredient ratio 95
Weighing 9.5% fluazinam technical product, 0.5% carvacrol technical product, 3% Nongru 2201#, 2% nonylphenol polyoxyethylene (EO = 10) ether phosphate, 2% Nongru 600# acid phospholipid, 10% cyclohexanone and 20% solvent oil S150, and mixing and dissolving to obtain an oil phase; adding 5% of ethylene glycol, 0.3% of xanthan gum, 0.03% of benzoic acid and deionized water to 100% to prepare a water phase, fully mixing the oil phase and the water phase, and performing high-shear emulsification to obtain the product.
2. Wettable powder
According to the formula requirement, various auxiliary agents, fillers and raw pesticide of a crusher are mixed by coarse powder in advance, and then the mixture is fully crushed and mixed in a jet mill to prepare wettable powder.
The embodiment is as follows: 20% fluazinam carvacrol (effective ingredient ratio 95
Weighing 19% fluazinam original drug, 1% carvacrol original drug, 4% calcium lignosulfonate, 4% NNO, 1% K-12, 5% white carbon black, adding calcium carbonate to 100%, mixing, and pulverizing to obtain the final product.
2. Biological Activity assay
The biological activity test is divided into an indoor toxicity test and a living body control effect test
Activity experiment 1: indoor toxicity determination of fluazinam and carvacrol compounded bactericide on konjac azalea disease
The pathogenic strain of the konjak white-azalea disease (Athalia rolfsii (GZCC 22-0087)) is provided by the biotechnological research institute of academy of agricultural sciences of Guizhou province, and the joint toxicity strength of different compound formulas is evaluated by adopting a co-toxicity coefficient method in the experiment, so that a reasonable proportion is defined.
The PDA culture medium preparation and fungus activation method comprises the following steps:
weighing 800 g of peeled potatoes, boiling, filtering, adding 80g of agar and 80g of glucose, uniformly mixing and dissolving, transferring 45mL of agar and 80g of glucose into a 100mL conical flask, sealing, autoclaving at 120 ℃ for 30min, and cooling for later use. The mycelia were picked and placed on sterilized PDA plates, and cultured in an incubator at 25 ℃ and 70% humidity for 3 days.
Formulation of the drug solution and EC 50 The test method comprises the following steps:
according to the predicted fluazinam and carvacrol EC 50 Values, established as a built test concentration gradient of 1. Mu.g/mL, 0.5. Mu.g/mL, 0.25. Mu.g/mL, 0.125. Mu.g/mL, and 0.0625. Mu.g/mL, respectively. The mass ratio of the fluazinam to the carvacrol tested is 95-20. The experiment is divided into 7 groups of compound combinations with different mass ratios, a culture medium containing dimethyl sulfoxide is used as a blank control CK, and 3 experiments in each group are arranged in parallel. And calculating the dosing amount of the fluazinam and the carvacrol according to the mass concentration ratio, respectively adding the dosing amounts into 5mL of deionized water containing Tween 80, uniformly mixing, pouring into the 45mL of PDA culture medium prepared above, uniformly mixing, pouring into 3 flat plates on average, and waiting for 2 hours for standby. The bacterial cake was inoculated on the above culture medium containing the drug solution by punching with a 4mm punch, and cultured in an incubator at 25 ℃ and 70% humidity for 3 days. Finally, the growth diameter of each cake was measured by a cross method. And the inhibition ratio I (%) was calculated according to the following formula:
the colony growth diameters after blank control and agent treatment are indicated as C and T, respectively.
Taking logarithm according to the concentration of the medicament and the antibacterial rate to obtain a regression line and EC 50 And a correlation coefficient R 2 . Obtaining an Actual Toxicity Index (ATI) according to a calculation method of the Sun Yunpei; the Theoretical Toxicity Index (TTI) is calculated according to the compounding ratio, and the co-toxicity coefficient (CTC) of the compounding formula is calculated according to the following formula.
S: EC of standard fungicide 50
M: EC of the mixture 50
TTI＝TI A ×P A +TI B ×P B
TI A The toxicity strength of a single agent of the A medicament; p A Is the percentage content of the A medicament in the mixture
TI B The virulence strength of a single agent of the B medicament; p is B Is the percentage content of the B medicament in the mixture
If the co-toxicity coefficient is more than 120, the synergistic effect is shown; 120-100 shows that the additive effect is achieved; antagonism is indicated below 100
TABLE 1 indoor toxicity test results of fluazinam and carvacrol compounded for konjac azalea disease a
“ a "Each group of experiments was repeated three times.
From the results in table 1, it can be seen that the synergistic effect is shown for the konjac azadirachtin when the fluazinam and the carvacrol are mixed in the mass ratio of 95 to 5 to 20. The result shows that the diniconazole and picoxystrobin have obvious synergistic effect according to the mass ratio of 95 50 The value is relatively optimal.
Activity experiment 2: in vivo efficacy test of devil's tongue
The konjak is planted in a flowerpot, and the experiment can be started after the growth height of the konjak is more than 20 cm. Under the growth condition of a large greenhouse, the protection and treatment effects of the compound combination after the potted konjak is inoculated with the konjak southern blight are evaluated. Day one, treatment group: cleaning rhizoma Amorphophalli root, cutting into 0.5cm thick pieces, perforating with a puncher to obtain 4mm fungus cake, inoculating on the surface of the slices, and covering with wet cotton; protection group: uniformly spraying each liquid medicine with the concentration of 8 mu g/mL on the section; the following day, treatment group: uniformly spraying the medicinal liquid with the concentration of 8 mug/mL on the cut surface; protection group: the procedure of the first day of the treatment group was repeated. Finally, the konjak was placed in a petri dish (the bottom of the dish was flooded with a small amount of water to the slices) and cultured in an incubator at an isothermal temperature of 25 ℃ for 4d.
DSA is the percentage of disease determined by cutting the root section and according to the ratio of the disease area to the total area, and the classification system classifies the cut surfaces as follows:
grade 1, DSA <5%;
grade 3, DSA is between 6 and 10 percent;
grade 5, DSA is between 11-20%;
grade 7, DSA is between 21 and 50 percent;
level 9, DSA >50%.
The disease index (C) is calculated as follows:
the disease control efficiency (I) is calculated as follows:
wherein C represents the disease index of the control group and T represents the disease index of the reconstituted group.
TABLE 2 in vivo preventive effect for preventing and treating konjaku white silk disease
a: the effective components of the aqueous emulsion are fluazinam and carvacrol which account for 10 percent, and the rest components are various auxiliary agents b: the wettable powder contains fluazinam and carvacrol as effective components accounting for 20% and the rest of the wettable powder containing the various assistants
c: blank control
As can be seen from the results in Table 2, the protective and therapeutic activities of the wettable powders are superior to those of the aqueous emulsions. Meanwhile, the protective activity of the aqueous emulsion and the wettable powder is superior to the therapeutic activity.
The indoor toxicity test and the living body control effect test show that:
(1) The bactericide composition reduces the problem of possible drug failure caused by ultraviolet, rainwater, drug adsorption and the like by adding a preparation. Compared with a single agent, the aqueous emulsion and the wettable powder respectively achieve the control effects of 69 percent and 84 percent of thiabendazole by 10 percent and 20 percent of content under the same mass concentration. Proved by experiments, the two medicines with different antibacterial mechanisms play a more obvious synergistic role in the actual prevention effect of resisting the fusarium konjac.
(2) By adding natural product, the cost of medicine can be reduced and low toxicity and low residue can be realized
(3) The composition consists of active ingredients with different antibacterial action mechanisms, and can delay the generation of drug resistance. The invention has no adverse effect on the leaves and the growth vigor of crops and meets the safety requirement of pesticide preparations.
The sterilization composition has good prevention effect on the konjac azalea disease.
Claims (6)
1. A germicidal composition, comprising: the components of the composition comprise: the mass ratio of fluazinam to carvacrol is 95-20.
2. A formulation suitable for agricultural use, characterized by: the preparation is a compound pesticide sterilization composition containing fluazinam and carvacrol.
3. The composition of claim 1, wherein: the composition is in the form of aqueous emulsion or wettable powder.
4. Use of the composition according to claim 1 for preventing and treating southern blight of konjak.
5. The composition of claim 3, wherein: the aqueous emulsion comprises the following components in percentage by mass:
4 to 9.5 percent of fluazinam;
6 to 0.5 percent of carvacrol;
3% agricultural milk 2201#;
2% nonylphenol polyoxyethylene ether phosphate;
2% of agricultural milk 600# phosphoric acid ester;
10% cyclohexanone;
20% mineral spirit S150;
mixing and dissolving to obtain an oil phase.
Mixing 5% of ethylene glycol;
0.3% xanthan gum;
deionized water is added to 100% to prepare a water phase.
Adding the oil phase into the water phase, fully mixing, and emulsifying by high shear to obtain the product.
6. The composition of claim 3, wherein: the wettable powder comprises the following components in parts by mass:
8 to 19 percent of fluazinam;
12 to 1 percent of carvacrol;
4% calcium lignosulfonate;
4％NNO；
1％K-12；
5% of white carbon black; adding light calcium to 100%, mixing uniformly and grinding to obtain the product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211145990.9A CN115886006A (en) | 2022-09-20 | 2022-09-20 | Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211145990.9A CN115886006A (en) | 2022-09-20 | 2022-09-20 | Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115886006A true CN115886006A (en) | 2023-04-04 |
Family
ID=86487299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211145990.9A Pending CN115886006A (en) | 2022-09-20 | 2022-09-20 | Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115886006A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053529A (en) * | 2012-12-29 | 2013-04-24 | 广东中迅农科股份有限公司 | Carvacrol-fluazinam pesticide composition |
| CN104686522A (en) * | 2015-02-13 | 2015-06-10 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Sterilizing composition containing sedaxane and fluazinam |
| CN109645010A (en) * | 2019-01-15 | 2019-04-19 | 四川农业大学 | A kind of pharmaceutical composition and its suspending agent preventing and treating Alternaria alternate |
-
2022
- 2022-09-20 CN CN202211145990.9A patent/CN115886006A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053529A (en) * | 2012-12-29 | 2013-04-24 | 广东中迅农科股份有限公司 | Carvacrol-fluazinam pesticide composition |
| CN104686522A (en) * | 2015-02-13 | 2015-06-10 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Sterilizing composition containing sedaxane and fluazinam |
| CN109645010A (en) * | 2019-01-15 | 2019-04-19 | 四川农业大学 | A kind of pharmaceutical composition and its suspending agent preventing and treating Alternaria alternate |
Non-Patent Citations (2)
| Title |
|---|
| 李利军 等: "魔芋白绢病病原菌生物学特性及中药提取物药物筛选", 西北农业学报, vol. 27, no. 10, pages 1518 - 1525 * |
| 覃丽萍 等: "氟啶胺与粘帚霉对防治茉莉白绢病的协同增效作用", 农药, vol. 46, no. 4, pages 269 - 271 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104839168B (en) | Bactericidal composition and its application | |
| CN107897198B (en) | Compound composition of zhongshengmycin and eugenol and application thereof | |
| CN115886006A (en) | Bactericidal composition compounded by fluazinam and carvacrol and used for resisting konjac southern blight | |
| CN101543221A (en) | Sterilizing composite compounded by mancozeb and tea saponin and application thereof | |
| CN107318865A (en) | The composition pesticide of captan and furametpyr compounding | |
| CN111109295B (en) | Pesticide composition with kasugamycin and nano-silver as active components and application thereof | |
| CN109418280A (en) | A kind of bactericidal composition containing copper 8-hydroxyquinolinate and Mancozeb | |
| CN107232217B (en) | Bactericide composition and application thereof | |
| CN107691456B (en) | Bactericidal composition containing fluoxastrobin and shenqinmycin | |
| CN106689181B (en) | Bactericidal composition containing fenpyrazamine | |
| CN114158565B (en) | Sterilization composition containing amifepin and chlorphenamine amide and application thereof | |
| CN104488898B (en) | Containing methylsulfonyl bacterium azoles and the complex composition of Xinjunan acetate and antibacterial | |
| CN115590026A (en) | Diniconazole and picoxystrobin compounded anti-konjac southern blight bactericidal composition | |
| CN115553296A (en) | Thiediazole and pyraclostrobin compounded konjak root rot resistant bactericidal composition | |
| CN115067362B (en) | Sterilization composition containing rhodopseudomonas palustris and tricyclazole | |
| CN104756996B (en) | Sterilization composition containing quinolone copper and ipconazole | |
| CN109479886A (en) | A kind of pesticide and preparation method containing prothioconazoles and probenazole | |
| CN104041502B (en) | A kind of containing cyflufenamid with the bactericidal composition of tridemorph | |
| CN115918666A (en) | 1,2-benzisothiazolin-3-one and carvacrol compounded konjak soft rot resistant pesticide suspending agent | |
| CN115590027A (en) | Tebuconazole and thymol compounded konjak root rot resistant bactericidal composition | |
| CN111903685B (en) | Bactericidal composition, bactericide and application | |
| CN115804383B (en) | Sterilization composition for preventing and treating corn ear rot and application thereof | |
| CN105685057A (en) | Sterilization composition containing tebuconazole and trichloroiminocyanuric acid | |
| CN110583669B (en) | Bactericidal composition for melon diseases | |
| CN105994321A (en) | Bactericidal composition containing oxolinic acid and zhongshengmycin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |