CN115885923A - Method for efficiently improving content of natural astaxanthin in eggs - Google Patents
Method for efficiently improving content of natural astaxanthin in eggs Download PDFInfo
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- CN115885923A CN115885923A CN202211659842.9A CN202211659842A CN115885923A CN 115885923 A CN115885923 A CN 115885923A CN 202211659842 A CN202211659842 A CN 202211659842A CN 115885923 A CN115885923 A CN 115885923A
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- astaxanthin
- eggs
- natural astaxanthin
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- feed
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- 239000001168 astaxanthin Substances 0.000 title claims abstract description 132
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- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 129
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to the field of agriculture, in particular to a method for efficiently improving the content of natural astaxanthin in eggs, which comprises the steps of feeding laying hens with feed for improving the natural astaxanthin in the eggs, wherein the feed for improving the content of the natural astaxanthin in the eggs consists of basic feed and special feed containing the natural astaxanthin; the method for inducing haematococcus pluvialis to efficiently accumulate astaxanthin by using epigallocatechin gallate greatly improves the yield of the haematococcus pluvialis to accumulate astaxanthin, thereby obviously improving the production efficiency; the levorotatory natural astaxanthin directly obtained is added into the feed to be beneficial to absorption of broiler chickens, so that the absorptivity of nutrients in the feed and the biotransformation rate of astaxanthin can be improved, and eggs rich in natural astaxanthin are obtained; furthermore, the epigallocatechin gallate can also enhance the function of broiler chickens and improve the egg yield, and the laid eggs also contain epigallocatechin gallate, so that the effect of the eggs is further enhanced.
Description
Technical Field
The invention relates to the field of agriculture, in particular to a method for efficiently improving the content of natural astaxanthin in eggs.
Background
Astaxanthin, also known as astaxanthin, is a ketocarotenoid, is fat-soluble and is easily soluble in organic solvents such as chloroform, acetone, and dimethyl sulfoxide. The natural astaxanthin is widely present in shrimps, crabs, salmonids, certain algae and fungi, has strong oxygen radical scavenging capacity, is a strongest antioxidant recognized at present, and has obvious effects on delaying senility, improving immunity, preventing and treating tumors, cardiovascular diseases, diabetes and the like. The existing production method of astaxanthin mainly comprises a chemical synthesis method, a natural extraction method and a biological synthesis method.
Astaxanthin can be divided into three classes according to molecular structure: l, D and D, the structure of astaxanthin has two symmetrical centers, and is C-3 and C-3' of two-end ring structures in the molecule. One asymmetric center can have two conformations, two asymmetric carbon atoms C-3 and C-3 'of astaxanthin can exist in the form of R or S, so that 3 stereoisomers are obtained, namely 3S, 3' S, 3'R, 3' S and 3R, 3'R isomers are mirror images, each enantiomer has opposite optical activity and can rotate plane polarized light to the left or the right, and the polarized light of the 3S and 3' S structure is called as left rotation; the polarized light of 3R and 3'R structure is rightward called dextrorotation, and the polarized light of 3S and 3' R structure has no optical rotation and is called racemization. Different sources of extraction of the raw materials determine different molecular structures of astaxanthin, resulting in different qualities of astaxanthin. The natural L-astaxanthin is 100% natural astaxanthin extracted from haematococcus pluvialis, is the astaxanthin which has the strongest oxidation resistance and the best effect of being taken by a human body at present; the artificially mixed and synthesized astaxanthin only accounts for about 25 percent of the levorotatory astaxanthin, the antioxidant effect only accounts for one fourth of 100 percent of the levorotatory astaxanthin, and the astaxanthin is not suitable for human bodies and is forbidden to be sold in the United states; the dextro-astaxanthin extracted by the rhodotorula fermentation method only has partial oxidation resistance, has high sugar content and has potential harm to human bodies; the astaxanthin extracted by crushing the shrimp shells is 100 percent of dextro-astaxanthin, has extremely low oxidation resistance and basically has no antioxidant aging effect on human bodies.
The Chinese patent with the application number of 201811339339.9 discloses a method for improving the content of natural astaxanthin in eggs, the feed consists of a basic feed and a premix mixed with the natural astaxanthin, the premix is formed by mixing natural haematococcus pluvialis, haematococcus pluvialis oil, feed-grade cuttlefish paste or shrimp paste, spirulina powder and edible lactic acid bacteria, and the basic feed is formed by mixing corn, wheat bran, bean cakes, fish oil, salt and methionine. The natural haematococcus pluvialis and haematococcus pluvialis oil are added into the traditional chicken feed to increase the proportion of esterified astaxanthin in eggs, the loss amount of astaxanthin is reduced in the egg cooking process, and cuttlefish paste or shrimp paste, spirulina powder and edible lactic acid bacteria are added to improve the feed flavor, improve the food digestibility and the biological value, greatly improve the astaxanthin content in the eggs, control the growth environment and promote the accumulation of astaxanthin.
The method directly adds natural haematococcus pluvialis and other materials into the feed, however, the astaxanthin content in the haematococcus pluvialis is only 1.5% -3.0%, the amount of astaxanthin absorbed by the chickens when the haematococcus pluvialis is directly added into the feed is less, and the natural astaxanthin content in eggs laid by the chickens is lower finally.
Disclosure of Invention
In order to solve the problems, the invention provides a method for efficiently increasing the content of natural astaxanthin in eggs. The natural astaxanthin is extracted after the haematococcus pluvialis is induced to efficiently accumulate the astaxanthin by utilizing epigallocatechin gallate, the astaxanthin with higher content and purity is obtained, and the eggs rich in the natural astaxanthin are obtained by feeding laying hens with mixed feed consisting of basic feed and special feed containing the natural astaxanthin.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a method for efficiently increasing the content of natural astaxanthin in eggs comprises the steps of feeding laying hens with feed for increasing the natural astaxanthin in eggs, wherein the feed for increasing the natural astaxanthin content in eggs consists of basic feed and special feed containing natural astaxanthin, and the special feed accounts for 3-8% of the total amount of the basic feed;
the special feed is formed by mixing trace elements, compound vitamins and natural astaxanthin;
the basic feed is prepared by mixing corn, wheat, fish oil, soybean meal, anhydrous calcium hydrophosphate, stone powder, salt and methionine;
the natural astaxanthin is extracted by inducing Haematococcus pluvialis to efficiently accumulate astaxanthin by utilizing epigallocatechin gallate.
Further, the steps of extracting the natural astaxanthin after inducing haematococcus pluvialis to efficiently accumulate the astaxanthin by using the epigallocatechin gallate are as follows:
s1, preparing an algae solution: culturing haematococcus pluvialis cells to logarithmic growth phase to obtain haematococcus pluvialis solution;
s2, astaxanthin accumulation: adding epigallocatechin gallate into the cultured algae solution, wherein the addition amount of epigallocatechin gallate is 7-10 mg/L; then culturing the above solution containing epigallocatechin gallate at 26-28 deg.C under 9000-10000lx for 8-10 days.
And S3, extracting natural astaxanthin of the algae cells by using an organic solvent.
Preferably, BG11 medium is used for culturing Haematococcus pluvialis cells in the step S1.
Preferably, in step S1, BG11 culture medium is adopted, the culture is carried out under the conditions of temperature of 20 +/-2 ℃, light intensity of 1800 +/-200 lx and light-dark ratio of 12h/12h, and the culture is carried out under a daylight lamp until the haematococcus pluvialis cells reach logarithmic growth phase, wherein the cell concentration reaches 3.0X 10 5 one/mL.
Preferably, the step S3 specifically includes: centrifuging the algae solution after astaxanthin accumulation for 5min at 4000r/min, and removing the supernatant to obtain algae cell precipitate; adding mixed solution containing 5% (v/v) KOH and 30% methanol to the algae cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break cell wall, adding ethanol: and (3) oscillating the mixed reagent of ethyl acetate (v: v) =3, centrifuging to collect supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
Preferably, the basic feed is prepared by mixing the following components in parts by weight: 60-65 parts of corn, 5-6 parts of wheat, 2-4 parts of fish oil, 18-20 parts of soybean meal, 1-3 parts of anhydrous calcium hydrophosphate, 6-8 parts of stone powder, 0.3-0.5 part of salt and 0.01-0.15 part of methionine. Preferably 62 parts of corn, 5 parts of wheat, 3 parts of fish oil, 18 parts of soybean meal, 2 parts of anhydrous calcium hydrophosphate, 7 parts of stone powder, 0.3 part of salt and 0.15 part of methionine.
Preferably, the special feed is prepared from trace elements, compound vitamins and a nutrition enhancer rich in astaxanthin according to the weight ratio of 1:2-4:1, and mixing the components in a ratio of 1.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for inducing haematococcus pluvialis to efficiently accumulate astaxanthin by utilizing epigallocatechin gallate, a raw material haematococcus pluvialis cell laboratory can be automatically cultured according to a conventional method, a small amount of epigallocatechin gallate is added into a cultured algae solution, natural astaxanthin in haematococcus pluvialis can be compositely induced to be rapidly and efficiently accumulated by combining strong light, the yield of the astaxanthin accumulated by the haematococcus pluvialis is greatly improved, and therefore the production efficiency is obviously improved; the directly obtained levorotatory natural astaxanthin is added into the feed to be beneficial to broiler chicken absorption, so that the absorptivity of feed nutrients and the biotransformation rate of astaxanthin can be improved, and eggs rich in natural astaxanthin are obtained; furthermore, the epigallocatechin gallate with the functions of resisting bacteria, viruses, oxidation, arteriosclerosis, thrombosis, angiogenesis, inflammation and tumors can also enhance the functions of broiler chickens and improve the egg yield, and the laid eggs also contain the epigallocatechin gallate, so that the effect of the eggs is further improved.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Description of materials: haematococcus pluvialis, which was obtained from natural astaxanthin, inc. of Jingzhou city and contained 2% natural astaxanthin.
Example 1
A method for efficiently increasing natural astaxanthin content of eggs comprises feeding laying hens with feed for increasing natural astaxanthin content of eggs, wherein the feed for increasing natural astaxanthin content of eggs comprises basic feed and special feed containing natural astaxanthin; wherein the special feed accounts for 5% of the total amount of the basic feed.
The special feed is formed by mixing trace elements, compound vitamins and natural astaxanthin; wherein the microelements are formed by mixing ferrous sulfate, copper sulfate, zinc sulfate and the like by weight; the compound vitamin is prepared by mixing microorganism A, vitamin D, microorganism E, vitamin B1, vitamin B2, vitamin B12, folic acid and the like; wherein the microelements, the compound vitamins and the nutrition enhancer rich in astaxanthin are mixed according to the weight ratio of 1:3:1 in proportion.
The basic feed is prepared by mixing the following components in parts by weight: 62 parts of corn, 5 parts of wheat, 3 parts of fish oil, 18 parts of bean pulp, 2 parts of anhydrous calcium hydrophosphate, 7 parts of stone powder, 0.3 part of salt and 0.15 part of methionine.
The natural astaxanthin is extracted by inducing haematococcus pluvialis to efficiently accumulate astaxanthin by utilizing epigallocatechin gallate. Wherein the epigallocatechin gallate is prepared from green tea by extracting, separating by chromatography, concentrating by evaporation, vacuum distilling, cooling, crystallizing, and drying.
The structural formula of the epigallocatechin gallate is as follows:
the method comprises the following steps of (1) inducing Haematococcus pluvialis to efficiently accumulate astaxanthin by using epigallocatechin gallate, and extracting natural astaxanthin, wherein the steps are as follows:
s1, preparing an algae solution: culturing in BG11 culture medium at 18 deg.C under 1600lx light intensity and 12h/12h light-to-dark ratioCulturing under fluorescent lamp until haematococcus pluvialis cell reaches logarithmic growth phase, wherein cell concentration reaches 3.0 × 10 5 Per mL;
s2, astaxanthin accumulation: adding epigallocatechin gallate into the cultured algae solution, wherein the addition amount of epigallocatechin gallate is 7 mg/L; then, the above-mentioned solution containing epigallocatechin gallate was cultured at 26 ℃ under 9000lx light intensity for 10 days.
S3, extracting natural astaxanthin of algae cells by using an organic solvent: centrifuging the algae solution after astaxanthin accumulation for 5min at 4000r/min, and removing the supernatant to obtain algae cell precipitate; adding a mixture containing 5% KOH and 30% methanol to the algal cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break the wall, adding ethanol: and (3) oscillating the mixed reagent of ethyl acetate (v: v) =3, centrifuging to collect supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
Example 2
The steps of extracting natural astaxanthin after inducing haematococcus pluvialis to efficiently accumulate astaxanthin by using epigallocatechin gallate comprise the following steps:
s1, preparing an algae solution: culturing by using BG11 culture medium under the conditions of 22 ℃ of temperature, 2000lx of light intensity and 12h/12h of light-to-dark ratio and culturing under a fluorescent lamp until haematococcus pluvialis cells reach logarithmic growth phase, wherein the cell concentration reaches 3.0 multiplied by 105 cells/mL;
s2, astaxanthin accumulation: adding epigallocatechin gallate into the cultured algae solution, wherein the addition amount of epigallocatechin gallate is 10 mg/L; then culturing the above solution containing epigallocatechin gallate at 28 deg.C under light intensity of 10000lx for 8d.
S3, extracting natural astaxanthin of algae cells by using an organic solvent: centrifuging the algae solution after astaxanthin accumulation for 5min at 4000r/min, and removing the supernatant to obtain algae cell precipitate; adding mixed solution containing 5% (v/v) KOH and 30% methanol to the algae cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break cell wall, adding ethanol: and (3) oscillating the mixed reagent of ethyl acetate (v: v) =3, centrifuging to collect supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
Example 3
The steps of extracting the natural astaxanthin after inducing the haematococcus pluvialis to efficiently accumulate the astaxanthin by utilizing the epigallocatechin gallate are as follows:
s1, preparing an algae solution: adopting BG11 culture medium, culturing at 20 deg.C under light intensity of 1800lx and light-dark ratio of 12h/12h under fluorescent lamp till Haematococcus pluvialis cells reach logarithmic phase, wherein the cell concentration reaches 3.0 × 105 cells/mL;
s2, astaxanthin accumulation: adding epigallocatechin gallate into the cultured algae solution, wherein the addition amount of epigallocatechin gallate is 8 mg/L; then, the above-mentioned solution containing epigallocatechin gallate was cultured at 27 ℃ under 9500lx light intensity for 9 days.
S3, extracting natural astaxanthin of algae cells by using an organic solvent: centrifuging the algae solution after astaxanthin accumulation for 5min at 4000r/min, and removing the supernatant to obtain algae cell precipitate; adding mixed solution containing 5% (v/v) KOH and 30% methanol to the algae cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break cell wall, adding ethanol: and (3) mixing the reagent with ethyl acetate (v: v) =3, oscillating, centrifuging, collecting supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
Comparative example 1
Compared with the example 1, the specific steps of extracting the natural astaxanthin are as follows:
s1, preparing an algae solution: culturing in BG11 culture medium at 20 deg.C under light intensity of 1800lx and light-to-dark ratio of 12h/12h under fluorescent lamp until Haematococcus pluvialis cells reach logarithmic phase, at which time the cell concentration reaches 3.0 × 10 5 Per mL;
s2, astaxanthin accumulation: the algal solution was cultured at 27 ℃ under a light intensity of 9500lx for 9 days.
S3, extracting natural astaxanthin of algae cells by using an organic solvent: centrifuging the algae solution after astaxanthin accumulation for 5min at 4000r/min, and removing the supernatant to obtain algae cell precipitate; adding mixed solution containing 5% (v/v) KOH and 30% methanol to the algae cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break cell wall, adding ethanol: and (3) oscillating the mixed reagent of ethyl acetate (v: v) =3, centrifuging to collect supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
And (4) relevant testing:
1. the contents of astaxanthin extracted in examples 1 to 3 and comparative examples 1 to 3 were measured by HPLC, and the results are shown in Table 1.
TABLE 1
| Sample (I) | Astaxanthin content (. Times.10) -3 g/L) |
| Example 1 | 40.6 |
| Example 2 | 42.5 |
| Example 3 | 42.3 |
| Comparative example 1 | 29.5 |
As can be seen from the test results in the table above, the content and purity of astaxanthin obtained by the culture methods of examples 1-3 are higher than those of comparative example 1, and it can be seen that Epigallocatechin gallate induces Haematococcus pluvialis to efficiently accumulate astaxanthin. With the best results from example 2.
2. The feeds prepared in examples 1 to 3 and comparative example 1 were each subjected to a laying hen feeding test.
The testing process comprises the following steps: uniformly mixing the base feed and the special feed which are proportioned and ground into powder in the examples 1-3 and the comparative example 1 in a mechanical stirring mode, and feeding laying hens respectively, wherein the base feed and the special feed are divided into 4 groups, each group contains 100 laying hens, each group correspondingly feeds a laying hen feed, each laying hen is fed with 100g of the feed every day, the laying hens are continuously fed for 25 days, the age of the laying hens is controlled to be 150-200 days, and the temperature of a henhouse is controlled to be 15-20 ℃. The obtained eggs were evaluated for the presence of astaxanthin and for the color of the egg yolk, and the sensory requirements for eggs containing natural astaxanthin are shown in table 2.
TABLE 2
The standard is suitable for detecting the content of total astaxanthin in the haematococcus pluvialis astaxanthin eggs by using an ultraviolet visible spectrophotometer.
A.1 Main Equipment and reagents
An ultraviolet-visible spectrophotometer;
a centrifuge (with centrifuge tube);
electronic balance (0.01 mg);
dimethylsulfoxide (analytical grade).
A.2 operating procedure
A.2.1 sample preparation
Accurately weighing 30mg of the uniformly mixed sample, putting the sample into a centrifuge tube, adding 2mL of dimethyl sulfoxide, soaking the sample in a 50 ℃ constant-temperature water bath for 15min, putting the sample into a centrifuge, centrifuging the sample at 350O rpm/min for 5min, and transferring the supernatant into a 100mL brown volumetric flask. And adding dimethyl sulfoxide into the residue again, soaking, extracting and centrifuging, repeating the steps until the solution is colorless, transferring all the solution into a volumetric flask, and then metering the volume to a scale by using the dimethyl sulfoxide for later use.
A.2.2 measurement read absorption
And (3) taking dimethyl sulfoxide as a blank control, reading an absorbance value at 489nm by using an ultraviolet-visible spectrophotometer, wherein the absorbance value is between 0.2 and 0.8, and if the absorbance value is not between, diluting and detecting.
A.2.3 calculation
The absorbance value is calculated as follows:
wherein: e (1% 1 cm) -1% (g/ml) of astaxanthin dimethyl sulfoxide solution mass extinction coefficient at 489nm (1908);
a (489) -absorbance value of sample at 489 nm;
v-volume of solution;
a is dilution multiple;
m-sample mass (g).
Note: 1. preparing astaxanthin egg yolk sample, namely separating egg yolk from egg white, and uniformly stirring the egg yolk to obtain a sample for later use.
2. The natural astaxanthin in the astaxanthin egg yolk is completely and fully extracted. Repeating the steps of soaking, extracting, centrifuging and transferring supernatant until the extraction solution is colorless and the residue is light yellow.
The results are shown in Table 2.
TABLE 3
| Sample(s) | Astaxanthin content (. Times.10) -3 g/L) |
| Example 1 egg | 5.6 |
| Example 2 egg | 6.5 |
| Example 3 egg | 6.3 |
| Comparative example 1 egg | 3.5 |
From the above test results, it can be seen that the eggs laid by the laying hens fed with the feeds prepared in examples 1 to 3 and comparative example 1 all contain astaxanthin.
Although embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A method for efficiently improving the content of natural astaxanthin in eggs is characterized in that a feed for improving the natural astaxanthin content of the eggs is added to a laying hen, the feed for improving the natural astaxanthin content of the eggs consists of a basic feed and a special feed containing natural astaxanthin, and the special feed accounts for 3-8% of the total amount of the basic feed;
the special feed is formed by mixing trace elements, compound vitamins and natural astaxanthin;
the basic feed is prepared by mixing corn, wheat, fish oil, soybean meal, anhydrous calcium hydrophosphate, stone powder, salt and methionine;
the natural astaxanthin is extracted by inducing Haematococcus pluvialis to efficiently accumulate astaxanthin by utilizing epigallocatechin gallate.
2. The method for efficiently increasing the content of natural astaxanthin in eggs according to claim 1, wherein the steps of extracting the natural astaxanthin after inducing Haematococcus pluvialis to efficiently accumulate astaxanthin by using epigallocatechin gallate (EGCG) comprise:
s1, preparing an algae solution: culturing haematococcus pluvialis cells to logarithmic growth phase to obtain haematococcus pluvialis solution;
s2, astaxanthin accumulation: adding epigallocatechin gallate into the cultured algae solution, wherein the addition amount of epigallocatechin gallate is 7-10 mg/L; then culturing the above solution containing epigallocatechin gallate at 26-28 deg.C under 9000-10000lx for 8-10 days.
And S3, extracting natural astaxanthin of the algal cells by using an organic solvent.
3. The method for efficiently increasing the content of natural astaxanthin in eggs according to claim 2, wherein BG11 medium is used for culturing Haematococcus pluvialis cells in the step S1.
4. The method according to claim 3, wherein in step S1, BG11 medium is used, the medium is cultured at 20 + -2 deg.C, light intensity of 1800 + -200 lx, and light-to-dark ratio of 12h/12h, and the medium is cultured under fluorescent lamp until Haematococcus pluvialis cells reach logarithmic growth phase, at which time the cell concentration reaches 3.0X 10 5 one/mL.
5. The method for efficiently increasing the content of natural astaxanthin in eggs according to claim 2, wherein the step S3 specifically comprises the following steps: centrifuging the algae solution accumulated with astaxanthin at 4000r/min for 5min, and removing supernatant to obtain algae cell precipitate; adding a mixture containing 5% KOH and 30% methanol to the algal cell precipitate to destroy chlorophyll, centrifuging, collecting precipitate, grinding the precipitate to break the wall, adding ethanol: and (3) oscillating the mixed reagent of ethyl acetate (v: v) =3, centrifuging to collect supernatant, concentrating the supernatant through vacuum rotary evaporation, and freeze-drying to obtain the natural astaxanthin.
6. The method for efficiently increasing the content of natural astaxanthin in eggs according to claim 1, wherein the basic feed is prepared by mixing the following components in parts by weight: 60-65 parts of corn, 5-6 parts of wheat, 2-4 parts of fish oil, 18-20 parts of soybean meal, 1-3 parts of anhydrous calcium hydrophosphate, 6-8 parts of stone powder, 0.3-0.5 part of salt and 0.01-0.15 part of methionine. Preferably 62 parts of corn, 5 parts of wheat, 3 parts of fish oil, 18 parts of soybean meal, 2 parts of anhydrous calcium hydrophosphate, 7 parts of stone powder, 0.3 part of salt and 0.15 part of methionine.
7. The method for efficiently increasing the content of natural astaxanthin in eggs according to claim 1, wherein the special feed is prepared from trace elements, compound vitamins and a nutrition enhancer rich in astaxanthin according to a weight ratio of 1:2-4:1, and mixing the components in a ratio of 1.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008131888A (en) * | 2006-11-28 | 2008-06-12 | Sanei Gen Ffi Inc | Method for improving light resistance of haematoccous pluvialis pigment |
| US20160312175A1 (en) * | 2015-04-21 | 2016-10-27 | Korea Advanced Institute Of Science And Technology | Cell coating method using compound comprising gallate group and lanthanoid metal salt or transition metal salt |
| WO2017130106A1 (en) * | 2016-01-25 | 2017-08-03 | Bio-P S.R.L. | Process for producing starch from microalgae |
| CN107058440A (en) * | 2017-05-05 | 2017-08-18 | 华中农业大学 | A kind of method of utilization rotating biological membrane reactor adhere-wall culture Haematococcus pluvialis production astaxanthin |
| CN108669337A (en) * | 2018-04-02 | 2018-10-19 | 山东鸣惠生物科技股份有限公司 | A kind of haematococcus health egg and its produce and use feed |
| CN109221792A (en) * | 2018-08-19 | 2019-01-18 | 无为县绿农果蔬农民专业合作社 | A kind of feed that cray immunity can be improved |
| CN109258966A (en) * | 2018-11-12 | 2019-01-25 | 中铭生物科技(深圳)有限公司 | A method of improving egg natural astaxanthin content |
| KR20220010720A (en) * | 2019-04-22 | 2022-01-26 | 퍼펙트 데이, 인크. | Egg substitutes and compositions comprising egg substitutes and methods of making the same |
-
2022
- 2022-12-23 CN CN202211659842.9A patent/CN115885923B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008131888A (en) * | 2006-11-28 | 2008-06-12 | Sanei Gen Ffi Inc | Method for improving light resistance of haematoccous pluvialis pigment |
| US20160312175A1 (en) * | 2015-04-21 | 2016-10-27 | Korea Advanced Institute Of Science And Technology | Cell coating method using compound comprising gallate group and lanthanoid metal salt or transition metal salt |
| WO2017130106A1 (en) * | 2016-01-25 | 2017-08-03 | Bio-P S.R.L. | Process for producing starch from microalgae |
| CN107058440A (en) * | 2017-05-05 | 2017-08-18 | 华中农业大学 | A kind of method of utilization rotating biological membrane reactor adhere-wall culture Haematococcus pluvialis production astaxanthin |
| CN108669337A (en) * | 2018-04-02 | 2018-10-19 | 山东鸣惠生物科技股份有限公司 | A kind of haematococcus health egg and its produce and use feed |
| CN109221792A (en) * | 2018-08-19 | 2019-01-18 | 无为县绿农果蔬农民专业合作社 | A kind of feed that cray immunity can be improved |
| CN109258966A (en) * | 2018-11-12 | 2019-01-25 | 中铭生物科技(深圳)有限公司 | A method of improving egg natural astaxanthin content |
| KR20220010720A (en) * | 2019-04-22 | 2022-01-26 | 퍼펙트 데이, 인크. | Egg substitutes and compositions comprising egg substitutes and methods of making the same |
Non-Patent Citations (3)
| Title |
|---|
| 张勇等: "雨生红球藻培养基筛选及诱导条件优化", 《科技风》, no. 1, 31 January 2022 (2022-01-31), pages 139 - 141 * |
| 邓薏: "近五年国内中药抗氧化作用研究进展(上)", 《中药药理与临床》, vol. 28, no. 6, 31 December 2012 (2012-12-31), pages 155 - 162 * |
| 顾聃等: ""抗氧化剂耦合非生物胁迫调控微藻油脂和类胡萝卜素合成的研究进展"", 《中国油脂》, vol. 47, no. 7, 30 September 2021 (2021-09-30), pages 132 - 138 * |
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