CN115867671A - 使用测序选择核酸配体 - Google Patents

使用测序选择核酸配体 Download PDF

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Publication number
CN115867671A
CN115867671A CN202180046730.3A CN202180046730A CN115867671A CN 115867671 A CN115867671 A CN 115867671A CN 202180046730 A CN202180046730 A CN 202180046730A CN 115867671 A CN115867671 A CN 115867671A
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China
Prior art keywords
nucleic acid
target
acid ligand
coupled
wells
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Pending
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CN202180046730.3A
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English (en)
Chinese (zh)
Inventor
C·查蒙
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Illumina Inc
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Illumina Inc
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Publication of CN115867671A publication Critical patent/CN115867671A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180046730.3A 2020-12-21 2021-12-16 使用测序选择核酸配体 Pending CN115867671A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063128669P 2020-12-21 2020-12-21
US63/128,669 2020-12-21
PCT/US2021/063835 WO2022140158A1 (en) 2020-12-21 2021-12-16 Selecting aptamers using sequencing

Publications (1)

Publication Number Publication Date
CN115867671A true CN115867671A (zh) 2023-03-28

Family

ID=79687195

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180046730.3A Pending CN115867671A (zh) 2020-12-21 2021-12-16 使用测序选择核酸配体

Country Status (11)

Country Link
US (1) US20230313273A1 (https=)
EP (1) EP4263858A1 (https=)
JP (1) JP2024500261A (https=)
KR (1) KR20230123873A (https=)
CN (1) CN115867671A (https=)
AU (1) AU2021409478A1 (https=)
CA (1) CA3183773A1 (https=)
IL (1) IL299522A (https=)
MX (1) MX2022014811A (https=)
TW (1) TW202235628A (https=)
WO (1) WO2022140158A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240209429A1 (en) * 2022-12-07 2024-06-27 Illumina, Inc. Monoclonal clustering using double stranded dna size exclusion with patterned seeding
CN116515975A (zh) * 2023-04-21 2023-08-01 深圳市真迈生物科技有限公司 利用测序筛选适配体的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102277353A (zh) * 2007-10-22 2011-12-14 普罗诺塔股份有限公司 选择适配体的方法
US20140303030A1 (en) * 2011-10-28 2014-10-09 Albert-Ludwigs-Universitaet Freiburg Method for identifying aptamers
CN109182455A (zh) * 2018-08-08 2019-01-11 淮海工学院 一种蛋白质核酸适配体的筛选方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2260103A4 (en) * 2008-03-12 2012-06-13 Univ Syracuse DIRECT SELECTION OF STRUCTURAL DEFINED APTAMERS
US20120088232A1 (en) * 2010-09-30 2012-04-12 Missouri State University Aptamer-Based Device For Detection Of Cancer Markers And Methods Of Use
EP4170031A1 (en) * 2012-10-23 2023-04-26 Caris Science, Inc. Aptamers and uses thereof
CA3057368A1 (en) * 2017-03-27 2018-10-04 Caris Science, Inc. Oligonucleotide probes and uses thereof
CN110892071A (zh) * 2017-03-30 2020-03-17 奥曼尼提纳米有限公司 用于功能性寡核苷酸选择的方法和组合物
US12258558B2 (en) * 2018-06-04 2025-03-25 Cz Biohub Sf, Llc Compositions and methods for screening aptamers
US11926820B2 (en) * 2019-09-13 2024-03-12 Google Llc Methods and compositions for protein and peptide sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102277353A (zh) * 2007-10-22 2011-12-14 普罗诺塔股份有限公司 选择适配体的方法
US20140303030A1 (en) * 2011-10-28 2014-10-09 Albert-Ludwigs-Universitaet Freiburg Method for identifying aptamers
CN109182455A (zh) * 2018-08-08 2019-01-11 淮海工学院 一种蛋白质核酸适配体的筛选方法

Also Published As

Publication number Publication date
TW202235628A (zh) 2022-09-16
EP4263858A1 (en) 2023-10-25
MX2022014811A (es) 2023-03-07
IL299522A (en) 2023-02-01
CA3183773A1 (en) 2022-06-30
JP2024500261A (ja) 2024-01-09
US20230313273A1 (en) 2023-10-05
KR20230123873A (ko) 2023-08-24
AU2021409478A1 (en) 2023-01-05
WO2022140158A1 (en) 2022-06-30

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