CN115862442A - Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process - Google Patents
Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process Download PDFInfo
- Publication number
- CN115862442A CN115862442A CN202211150017.6A CN202211150017A CN115862442A CN 115862442 A CN115862442 A CN 115862442A CN 202211150017 A CN202211150017 A CN 202211150017A CN 115862442 A CN115862442 A CN 115862442A
- Authority
- CN
- China
- Prior art keywords
- vibration
- swing
- plate
- parallel plate
- cam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000017531 blood circulation Effects 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000033001 locomotion Effects 0.000 title claims abstract description 20
- 230000008569 process Effects 0.000 title claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims abstract description 92
- 230000017105 transposition Effects 0.000 claims abstract description 9
- 239000012530 fluid Substances 0.000 claims description 12
- 238000007906 compression Methods 0.000 claims description 5
- 230000006835 compression Effects 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000010355 oscillation Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 11
- 210000004204 blood vessel Anatomy 0.000 description 8
- 210000001772 blood platelet Anatomy 0.000 description 7
- 230000003534 oscillatory effect Effects 0.000 description 7
- 238000010008 shearing Methods 0.000 description 5
- 238000004088 simulation Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 238000002513 implantation Methods 0.000 description 4
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002980 postoperative effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000002792 vascular Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000009194 climbing Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000004791 biological behavior Effects 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003433 contraceptive agent Substances 0.000 description 1
- 230000002254 contraceptive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 208000037803 restenosis Diseases 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 125000003003 spiro group Chemical group 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Images
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The invention discloses a parallel flat plate flow cavity system for simulating a blood flow environment in a human body movement process, which comprises a parallel flat plate flow cavity, a swinging assembly and a vibrating assembly, wherein the swinging assembly is arranged on the parallel flat plate flow cavity; the swing assembly comprises a transposition mechanism, a jacking mechanism, a connecting bracket, a swing mechanism and a crank connecting rod mechanism; the vibration assembly comprises a vibration support frame, a pushing bottom plate and a cam slewing mechanism; the vibration guide columns are arranged at four corners of the bottom of the connecting support respectively, the positions, corresponding to the vibration guide columns, on the pushing bottom plate and the vibration support frame are respectively provided with a fixed sleeve and a movable sleeve which are matched, and the vibration guide columns penetrate through the corresponding movable sleeves and then are sleeved with the fixed sleeves and fixed. The system can be used for simulating a vibration and oscillation environment independently or jointly, and can effectively simulate the platelet adhesion condition on the stent in a blood flow environment after the stent is implanted, thereby providing beneficial assistance for related medical research.
Description
Technical Field
The invention belongs to the technical field of cell biomechanics experimental devices, particularly relates to a parallel flat plate flow cavity system for simulating a blood flow environment in a human body movement process, and particularly relates to a parallel flat plate flow cavity system for simulating a vibration and swing environment.
Background
Relevant researches show that biological movement of all organs, tissues, cells and the like on all levels is performed in a certain mechanical environment. The influence of the fluid shearing force on cells is an important subject of the current research on cell biology and cell mechanics, but due to the complexity of the physiological environment in human body, the in vitro experiment becomes the main means of the research on cell mechanics, wherein the parallel flat plate flow cavity is one of the main devices for researching the change rule of the cells under the action of the shearing stress.
The parallel flat plate flow chamber is a flat flow chamber with the height far smaller than the transverse width and the longitudinal length, constant shearing force is provided by hydrostatic pressure with different height differences, or transient shearing force is provided by an activated pump to generate pressure difference between the inflow pipe and the outflow pipe, so that cells in the flow chamber are subjected to uniform or pulse shearing force, and then the flow condition of fluid inside the flow chamber and the stress condition of the cells can be observed after the flow chamber is matched with a related optical instrument.
In recent years, the incidence of cardiovascular and cerebrovascular diseases has increased year by year, and the use of vascular stents has also become widespread. Along with the continuous development of blood vessel support technique, various novel naked supports, medicine coating support constantly appear, have improved the later stage unobstructed rate that artery blood vessel intervenes treatment greatly, but the later stage incidence of blockking up again is far above other positions of blood vessel, based on the emergence of this kind of condition, the urgent parallel flat plate flow chamber that needs to study a blood flow environment that can simulate the support postoperative for observe the adhesion condition of postoperative platelet on the support and implant the back restenosis problem with the research support. However, the existing parallel flat plate flow chamber is mainly used for researching cell adhesion characteristics, cannot be well used for research under the actual stent implantation environment, and has obvious simulation scene limitation. Meanwhile, because the blood vessel of the human body is in a motion state at every moment after the stent is implanted, the simple static simulation system is difficult to be used for simulating the adhesion condition of the blood platelets on the stent in the blood flow environment after the stent is implanted.
Chinese patent CN 103881899A discloses a parallel plate flow chamber system simulating oscillatory blood flow shear stress environment, which changes the switching frequency of the electromagnetic valve and the size and proportion of the post-flow load of the parallel plate flow chamber to realize oscillatory flow environment with different combinations of average value, oscillatory amplitude and oscillatory frequency inside the parallel plate flow chamber, thereby applying oscillatory shear stress with different characteristics to the vascular endothelial cells cultured at the bottom of the parallel plate flow chamber, and further quantitatively analyzing the biological behavior and mechanism of the oscillatory shear stress for quantitatively regulating and controlling the adherent cells in vitro. However, the system mainly solves the problems of complex structure, high manufacturing cost and difficult control process of oscillatory flow of the traditional mechanical and blade type pulsating flow generation pump, and still cannot accurately simulate various motion modes, so that a parallel flat plate flow chamber experimental device capable of simulating multi-modal motion modes is still needed to be researched.
Disclosure of Invention
The invention aims to solve the defects in the prior art and discloses a parallel flat plate flow cavity system for simulating a blood flow environment in a human body movement process, which can effectively simulate the platelet adhesion condition on a stent in the blood flow environment after the stent is implanted by controlling a swinging component and a vibrating component.
The technical scheme of the invention is as follows: a parallel flat plate flow cavity system for simulating blood flow environment in the human body movement process comprises a parallel flat plate flow cavity, a swinging assembly and a vibrating assembly which are sequentially arranged; the swing assembly comprises a transposition mechanism, a jacking mechanism, a connecting bracket, a swing mechanism and a crank connecting rod mechanism; the transposition mechanism is fixedly arranged above the jacking mechanism and comprises a cavity containing frame and a hinged support movably connected with the cavity containing frame; the jacking mechanism comprises an active jacking mechanism and a passive jacking mechanism which are oppositely arranged at two sides of the connecting bracket; the active jacking mechanism and the passive jacking mechanism are respectively connected with the bottom surfaces of two ends of the cavity containing frame, and the active jacking mechanism is in transmission connection with the crank link mechanism; the swing slewing mechanism is arranged on the connecting bracket; the vibration assembly comprises a vibration support frame, a pushing bottom plate and a cam slewing mechanism; the vibration guide columns penetrate through the corresponding movable sleeves and are sleeved and fixed with the fixed sleeves, and vibration springs are sleeved on the vibration guide columns and located between the movable sleeves and the fixed sleeves in a surrounding mode.
Further, initiative climbing mechanism includes first spout, first slider and first lifting rod, and first slider sliding connection just can follow its longitudinal sliding in first spout, and the bottom and the fixed bottom that meets of first lifting rod, top pass on the first spout behind the movable hole of adaptation link up the setting through the bottom surface of jacking head with cavity holding frame, the first slider outside end is connected with crank link mechanism's one end is articulated.
Further, passive climbing mechanism includes the second spout, second slider and second jacking pole, second slider sliding connection just can follow its longitudinal sliding in the second spout, the bottom and the fixed meeting of second slider of second jacking pole, the top passes behind the movable hole of the adaptation on the second spout through the bottom surface linking setting of jacking head and cavity holding frame, it has reset spring to fit with a contraceptive ring at the second jacking pole, reset spring establishes between the inner chamber top surface of second slider top surface and second spout and reset spring both ends respectively with second slider top surface and the fixed meeting of second spout inner chamber top surface.
Furthermore, a cross-shaped groove is arranged in the cavity containing frame, and the size of the groove is matched with that of the parallel flat plate flow cavity.
Furthermore, the swing mechanism comprises a swing shaft and a swing bearing connected to the swing shaft, one end of the swing shaft is connected with the crank link mechanism, the other end of the swing shaft is connected with an external motor, the swing bearing is arranged in a swing bearing seat, the swing bearing seat comprises a swing upper shaft seat and a swing lower shaft cover, the swing upper shaft seat is fixedly connected with the bottom surface of the connecting support, and the swing lower shaft cover is fixedly connected with the swing upper shaft seat.
Furthermore, the vibration supporting frame is fixed on the bottom plate, the cam rotating mechanism is arranged below the pushing bottom plate and comprises a cam, a vibration bearing and a cam shaft, the vibration bearing is connected to a vibration bearing seat, the cam is arranged between the two vibration bearings and is connected to the cam shaft in a key mode, the vibration bearing is arranged in a bearing cover on the vibration bearing seat, the vibration bearing seat is fixed on the bottom plate, and one end of the cam shaft is connected with an external motor in a key mode.
Furthermore, a parking pin hole is formed in the second sliding groove, a parking hole is also formed in the position, corresponding to the parking pin hole, of the second sliding block, and the parking bolt sequentially penetrates through the second sliding groove, the parking pin hole in the second sliding block and the parking hole to achieve parking of the swing assembly.
Furthermore, the camshaft and the vibration support frame are correspondingly provided with pin holes, when the cam reaches the maximum vibration position, the vibration pins are sequentially inserted into the pin holes in the camshaft and the vibration support frame, and the vibration assembly is limited and fixed.
Furthermore, the parallel flat plate flow cavity comprises an upper perspective plate, an upper parallel plate, a lower parallel plate and a lower perspective plate which are fixedly connected in sequence, the upper perspective plate and the lower perspective plate are made of transparent materials, a compression gasket is clamped between the upper parallel plate and the lower parallel plate, and rubber gaskets are arranged between the matching surfaces of the upper perspective plate and the upper parallel plate and between the matching surfaces of the lower parallel plate and the lower perspective plate; the upper perspective plate is uniformly provided with a plurality of threaded holes for being in threaded connection with slotted headless shaft position screws, the corresponding positions of the two sides of the lower parallel plate are respectively provided with a fluid inlet and a fluid outlet for being externally connected with a hollow circular tube, a group of positioning blocks are respectively and symmetrically fixedly arranged in cavities formed by the two ends of the upper parallel plate and the lower parallel plate after being connected, and the outer side ends of the positioning blocks are provided with connecting guide pillars.
Furthermore, three connecting grooves are respectively arranged on the peripheral frame edges of the four sides of the cavity containing frame, the connecting groove at the middle position on the same frame edge is used for the passing of the hollow circular tube, and the arrangement positions of the connecting grooves at the positions of the two sides are matched with the arrangement position and the size of the connecting guide post on the parallel flat plate flow cavity.
The invention has the beneficial effects that:
1. the parallel flat plate flow cavity system for simulating the blood flow environment in the human body movement process can be used for simulating the vibration and swing environment independently or jointly, namely the platelet adhesion condition of a support in the blood flow environment after the support is implanted can be effectively simulated, the simulation scene is closer to the real condition, the obtained data is more real and representative, the working scene of the parallel flat plate flow cavity can be effectively expanded, and beneficial assistance is provided for relevant medical research;
2. the swing assembly and the vibration assembly disclosed by the application respectively utilize the parking bolt and the vibration bolt to realize the moving and stopping control of the corresponding assemblies, the design can improve the stability of the corresponding assemblies in a non-working state, and can also ensure that the working effect of the assemblies in a working state is not interfered;
3. the cross-shaped groove in the cavity containing frame provides two perpendicular swinging directions for the parallel flat plate flow cavity, and the cavity swinging effects in different directions can be simulated after the parallel flat plate flow cavity is arranged in the groove hole in the corresponding direction;
4. the spiro union several fluting does not have the first axle position screw to be used for the winding of blood vessel support silk spacing on parallel dull and stereotyped flow chamber in this application, can be used to the blood flow environment of direct simulation support implantation postoperative, and can be used to simulate the blood flow environment under the normal condition again when spinning up six fluting does not have the first axle position screw to its bottom surface and the position that the bottom surface of last perspective board flushes, and this design has fully promoted system's suitability.
Drawings
FIG. 1 is an isometric view of a parallel plate flow chamber system simulating the blood flow environment during human movement;
FIG. 2 is an isometric view of a parallel plate flow chamber;
FIG. 3 is a view of the internal structure of a parallel plate flow chamber;
FIG. 4 is a schematic view of the maximum swing position of the swing assembly;
FIG. 5 is a schematic view of a parallel plate flow chamber and a swing assembly connection;
FIG. 6 is an internal structure view of the swing turning mechanism;
FIG. 7 is a schematic view of the vibration assembly in a maximum amplitude position and secured thereto;
FIG. 8 is an internal structural view of the cam rotating mechanism;
wherein, 1-parallel flat plate flow cavity, 2-swinging component, 3-vibrating component;
11-upper perspective plate, 12-upper parallel plate, 13-lower parallel plate, 14-lower perspective plate, 15-compression gasket, 16-rubber gasket, 17-slotted headless shaft position screw, 18-fluid inlet, 19-connecting guide column;
21-a transposition mechanism, 22-an active jacking mechanism, 23-a passive jacking mechanism, 24-a connecting support, 25-a swing rotation mechanism, 26-a crank connecting rod mechanism and 27-a parking bolt;
211-cavity housing frame, 212-hinged support;
2111-connecting grooves;
221-a first chute, 222-a first slide block, 223-a first lifting rod, 224-a lifting head;
231-a second sliding chute, 232-a second sliding block, 233-a second jacking rod and 234-a return spring;
251-oscillating bearing, 252-oscillating shaft, 253-oscillating bearing seat;
2531-swing upper shaft seat, 2532-swing lower shaft cover;
261-connecting rod, 262-crank;
31-bottom plate, 32-vibration support frame, 33-pushing bottom plate, 34-cam rotating mechanism, 35-vibration guide column, 36-fixed sleeve, 37-movable sleeve, 38-vibration spring and 39-vibration bolt;
341-cam, 342-oscillating bearing, 343-camshaft, 344-oscillating bearing seat, 345-cam cap.
Detailed Description
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications and substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit of the invention.
Example 1:
in order to better simulate the stent implantation environment close to the actual condition, the embodiment provides a parallel flat plate flow chamber system capable of simulating the vibration and swing environment; comprises a parallel flat plate flow cavity 1, a swinging component 2 and a vibrating component 3 which are arranged from top to bottom in sequence.
The swing assembly 2 comprises a transposition mechanism 21, an active jacking mechanism 22, a passive jacking mechanism 23, a connecting support 24, a swing rotation mechanism 25 and a crank connecting rod mechanism 26, wherein the active jacking mechanism 22 and the passive jacking mechanism 23 are oppositely arranged on two sides of the connecting support 24, the transposition mechanism 21 is fixedly arranged above the active jacking mechanism 22 and the passive jacking mechanism 23, the swing rotation mechanism 25 is arranged on the connecting support 24, one end of the crank connecting rod mechanism 26 is connected with the swing rotation mechanism 25, and the other end of the crank connecting rod mechanism 26 is connected with the active jacking mechanism 22.
The transposition mechanism 21 comprises a cavity containing frame 211 and a hinged support 212, wherein a cross-shaped groove is formed in the cavity containing frame 211 and can be used for internally arranging parallel flat plate flow cavities 1 in two perpendicular directions so as to simulate the cavity swinging conditions in different directions; the two ends of the top of the hinge bracket 212 are hinged to the two opposite outer surfaces of the cavity accommodating frame 211, and the cavity accommodating frame 211 in the initial state can be in a horizontal stable state;
the bottom surfaces of the other two opposite surfaces of the cavity accommodating frame 211 are respectively connected with the active jacking mechanism 22 and the passive jacking mechanism 23, the active jacking mechanism 22 comprises a first sliding groove 221, a first sliding block 222 and a first jacking rod 223, the first sliding block 222 is slidably connected in the first sliding groove 221 and can longitudinally slide along the first sliding groove, the bottom of the first jacking rod 223 is fixedly connected with the first sliding block 222, the top of the first jacking rod 223 penetrates through a movable hole of the first sliding groove 221 and then is connected with the bottom surface of the cavity accommodating frame 211 through a jacking head 224, and the outer side end of the first sliding block 222 is hinged with one end of the crank link mechanism 26. The passive jacking mechanism 23 includes a second sliding groove 231, a second sliding block 232 and a second jacking rod 233, the second sliding block 232 is slidably connected in the second sliding groove 231 and can slide longitudinally along the second sliding block, the bottom of the second jacking rod 233 is fixedly connected with the second sliding block 232, the top of the second jacking rod 233 passes through a movable hole of the second sliding groove 231 and then is connected with the bottom surface of the cavity accommodating frame 211 through a jacking head, a return spring 234 is sleeved on the second jacking rod 233, the return spring 234 is arranged between the top surfaces of the inner cavities of the second sliding grooves 231 on the top surfaces of the second sliding blocks 232, and two ends of the return spring 234 are respectively fixedly connected with the top surfaces of the second sliding blocks 232 and the inner cavities of the second sliding grooves 231.
The crank-link mechanism 26 includes a connecting rod 261 and a crank 262, one end of the connecting rod 261 is hinged with the outer surface of the first slider 222, the other end is hinged with one end of the crank 262, and the other end of the crank 262 is connected with the swing-swing mechanism 25.
The swing rotation mechanism 25 comprises a swing bearing 251 and a swing shaft 252, the swing bearing 251 is connected to the swing shaft 252, two ends of the swing shaft 252 are provided with key slots, one end of the swing shaft 252 is connected with a crank 262 key in the crank link mechanism 26, and the other end is connected with an external motor; the swing bearing 251 is arranged in the swing bearing seat 253, the swing bearing seat 253 comprises a swing upper shaft seat 2531 and a swing lower shaft cover 2532, the swing upper shaft seat 2531 is fixedly connected with the bottom surface of the connecting bracket 24, and the swing lower shaft cover 2532 is fixedly connected with the swing upper shaft seat 2531; the connecting bracket 24 is fixedly connected to the inner side surfaces of the first sliding groove 221 and the second sliding groove 231.
A parking pin hole is formed in the second sliding groove 231, a parking hole is also formed in the second sliding block 232 at a position corresponding to the parking pin hole, and the parking pin 27 sequentially penetrates through the second sliding groove 231, the parking pin hole formed in the second sliding block 232 and the parking hole to realize the parking of the swing assembly 2.
The vibration assembly 3 comprises a bottom plate 31, a vibration support frame 32, a pushing bottom plate 33 and a cam rotation mechanism 34, wherein the vibration support frame 32 is fixed on the bottom plate 31, four corners of the bottom of the connecting support 24 are respectively provided with a vibration guide column 35, a fixed sleeve 36 matched with the vibration guide column 35 is arranged on the pushing bottom plate 33, a movable sleeve 37 matched with the vibration guide column 35 is arranged on the vibration support frame 32 at a position corresponding to the vibration guide column 35, the bottom end of the vibration guide column 35 penetrates through the correspondingly arranged movable sleeve 37 and then is sleeved and fixed with the fixed sleeve 36 at the corresponding position of the bottom, and a vibration spring 38 is annularly sleeved on the vibration guide column 35 and positioned between the movable sleeve 37 and the fixed sleeve 36 for realizing reciprocating vibration. The cam rotating mechanism 34 is correspondingly arranged below the pushing bottom plate 33, the cam rotating mechanism 34 comprises a cam 341, a vibration bearing 342 and a cam shaft 343, the vibration bearing 342 is connected to a vibration bearing seat 344, the cam 341 is arranged between the two vibration bearings 342 and is connected to the cam shaft 343 in a key mode, the vibration bearing 342 is arranged in a bearing cover on the vibration bearing seat 344, the vibration bearing seat 344 is fixed on the bottom plate 31, and one end of the cam shaft 343 is connected to an external motor (not shown in the figure) in a key mode.
In order to prevent the axial play of the cam 341, two cam caps 345 are fixedly coupled to both sides of the cam 341.
The cam shaft 343 and the vibration support frame 32 are correspondingly provided with pin holes, and when the cam 341 reaches the maximum vibration position, the vibration bolt 39 can be sequentially inserted into the pin holes on the cam shaft 343 and the vibration support frame 32, so that the vibration assembly 3 is limited and fixed.
The parallel flat plate flow chamber 1 is a main observation component, comprising an upper perspective plate 11, an upper parallel plate 12, a lower parallel plate 13 and a lower perspective plate 14 which are fixedly connected in sequence through a bolt and nut component, and a certain pretightening force is required to be applied during connection, the upper perspective plate 11 and the lower perspective plate 14 are both made of transparent acrylic plates, a compression gasket 15 is clamped between the upper parallel plate 12 and the lower parallel plate 13 for sealing, and rubber gaskets 16 are arranged between the connection surfaces of the upper perspective plate 11 and the upper parallel plate 12 and between the connection surfaces of the lower parallel plate 13 and the lower perspective plate 14 in order to further improve the sealing effect; six threaded holes are uniformly distributed on the upper perspective plate 11 and used for being in threaded connection with six slotted headless shaft position screws 17, a fluid inlet 18 and a fluid outlet with the diameter of 2mm are respectively arranged at the corresponding positions of the two sides of the lower parallel plate 13 and used for being externally connected with a hollow circular tube, a group of positioning blocks are respectively and symmetrically arranged in cavities formed at the two ends after the upper parallel plate 12 and the lower parallel plate 13 are connected, the positioning blocks are fixedly connected with the upper parallel plate 12 and the lower parallel plate 13 through pin shafts, and connecting guide columns 19 are arranged at the outer ends of the positioning blocks.
Three connecting grooves 2111 are respectively arranged on the peripheral frame edges of four sides of the cavity accommodating frame 211, the connecting groove 2111 at the middle position on the same frame edge is used for the passing of a hollow circular tube so that the hollow circular tube can be smoothly butted with a fluid inlet 18/a fluid outlet on the lower parallel plate 13, the arrangement positions of the connecting grooves 2111 at the two side positions are matched with the arrangement position and the size of a connecting guide pillar 19 on the parallel flat plate flowing cavity 1, and the parallel flat plate flowing cavity 1 can be connected in the cavity accommodating frame 211 in a clamping mode through the connecting guide pillar 19.
The specific workflow of the system is as follows:
firstly, on six slotted headless axial position screws 17 on a parallel flat plate flow cavity 1, a stent wire winding mode is adopted to simulate the vascular environment after stent implantation. Of course, if the system needs to be used for researching and simulating the blood flow environment in a normal state, the six slotted headless shaft position screws 17 are screwed to the positions of the bottom surfaces of the screws and the bottom surface of the upper perspective plate 11.
After the swing direction of the test is selected, the parallel flat plate flow chamber 1 is placed in the groove in the corresponding direction in the chamber body accommodating frame 211.
During the test, the fluid flowing condition and the cell stress condition on the blood vessel stent wire in the test process can be observed through the upper perspective plate 11 made of the transparent material.
The swing component 2 and/or the vibration component 3 can be used for simulating the blood flow condition after the blood vessel stent is implanted and the adhesion condition of platelets in the moving process.
According to the blood vessel stent vibration control method, the parking bolt 27 and the vibration bolt 29 are utilized to realize the dynamic stop control of corresponding movement, if the swing assembly 2 needs to be operated independently to study the blood flow condition and the platelet adhesion condition after the blood vessel stent is implanted in the swing movement process, the vibration bolt 39 is firstly inserted in place to realize the parking of the vibration assembly 3, then the parking bolt 27 is pulled out from the second sliding groove 231 to enable the swing assembly 2 to be in a normal operation state, and the working mode of the swing assembly 2 is as follows: firstly, an external motor connected with the swing rotation mechanism 25 works to drive the swing shaft 252 to rotate so as to drive the crank link mechanism 26 to operate, under the action of the crank link mechanism 26, the first slider 222 moves upwards along the first chute 221, and then the first lifting rod 223 drives the cavity containing frame 211 on the side to lift upwards, the cavity containing frame 211 on one side of the passive lifting mechanism 23 sinks passively, the cavity containing frame 211 inclines at an angle, the cavity containing frame 211 on one side of the passive lifting mechanism 23 sinks to drive the second lifting rod 233 and the second slider 232 to sink, and then the return spring 234 is stretched, and when the first slider 222 rises to the highest limit position, the parallel flat plate flow chamber 1 finishes the swing work in a single direction; after that, the crank link mechanism 26 starts to drive the first slider 222 to slide downwards, the parallel plate flow chamber 1 starts to reset and swings in the other direction, in this process, the reset spring 234 in the stretching state can drive the driven jacking mechanism 23 to quickly return to the original position under the action of the elastic restoring force, until the parallel plate flow chamber 1 swings to the limit position in the other direction, the reset spring 234 is in the pressing state, the reset spring 234 in the compression state can help the parallel plate flow chamber 1 to return to the original position again under the action of the elastic restoring force in the following process, and then the swing control on the parallel plate flow chamber 1 is realized repeatedly.
If the vibration component 2 needs to be operated independently to study the blood flow condition after the vascular stent is implanted and the adhesion condition of platelets in the vibration motion process, the parking bolt 27 is kept in the state of being inserted in place to realize the parking of the swing component 2, at this time, the vibration bolt 39 is pulled off from the cam shaft 343 to enable the vibration component 3 to be in the state of normal operation, and the working mode of the vibration component 3 is as follows: the external motor connected to the cam rotating mechanism 34 works to drive the cam 341 to rotate, and in the rotating process of the cam 341, the pushing bottom plate 33 connected to the cam 341 is driven to move up and down, so as to drive the connecting bracket 24 to vibrate up and down, and the vibrating spring 38 on the vibrating guide post 35 helps the pushing bottom plate 33 to rapidly and stably reset so as to improve the stability of the vibrating process, thereby finally realizing the effect of vibrating the parallel plate flow chamber 1.
Certainly, the oscillating module 2 and the vibrating module 3 do not only operate independently, if the oscillating and vibrating operations of the parallel flat plate flow chamber 1 need to be completed simultaneously, the oscillating pin 39 and the parking pin 27 only need to be removed simultaneously to enable the oscillating module 2 and the vibrating module 3 to be in a state of normal operation, then the operations of oscillating and oscillating are realized according to the related operating modes, and when the experiment is finished, the oscillating pin 39 and the parking pin 27 are inserted in place again to enable the system to be stable.
Meanwhile, when the system is used for a simulation experiment, the swinging direction can be changed, namely, the moving direction of the parallel flat plate flow cavity 1 is changed by placing the parallel flat plate flow cavity 1 on the cavity containing frame 211 through the slotted holes in different directions of the cross-shaped groove, so that the swinging mode can be changed.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. However, the above description is only an example of the present invention, the technical features of the present invention are not limited thereto, and any other embodiments that can be obtained by those skilled in the art without departing from the technical solution of the present invention should be covered by the claims of the present invention.
Claims (10)
1. The parallel flat plate flow cavity system for simulating the blood flow environment in the human body movement process is characterized by comprising a parallel flat plate flow cavity, a swinging assembly and a vibrating assembly which are sequentially arranged;
the swing assembly comprises a transposition mechanism, a jacking mechanism, a connecting bracket, a swing mechanism and a crank connecting rod mechanism;
the transposition mechanism is fixedly arranged above the jacking mechanism and comprises a cavity containing frame and a hinged support movably connected with the cavity containing frame;
the jacking mechanism comprises an active jacking mechanism and a passive jacking mechanism which are oppositely arranged at two sides of the connecting bracket;
the active jacking mechanism and the passive jacking mechanism are respectively connected with the bottom surfaces of two ends of the cavity containing frame, and the active jacking mechanism is in transmission connection with the crank link mechanism;
the swing mechanism is arranged on the connecting bracket;
the vibration assembly comprises a vibration support frame, a pushing bottom plate and a cam slewing mechanism;
the vibration guide column is sleeved and fixed with the fixed sleeve after penetrating through the corresponding movable sleeve, and a vibration spring is sleeved on the vibration guide column and positioned between the movable sleeve and the fixed sleeve.
2. The parallel plate flow chamber system for simulating the blood flow environment during human body movement according to claim 1, wherein the active jacking mechanism comprises a first sliding groove, a first sliding block and a first jacking rod, the first sliding block is slidably connected in the first sliding groove and can slide longitudinally along the first sliding groove, the bottom of the first jacking rod is fixedly connected with the first sliding block, the top of the first jacking rod passes through a movable hole matched with the first sliding groove and then is connected with the bottom surface of the chamber accommodating frame through a jacking head, and the outer side end of the first sliding block is hinged with one end of the crank link mechanism.
3. The system according to claim 1, wherein the passive lifting mechanism comprises a second sliding slot, a second sliding block and a second lifting rod, the second sliding block is slidably connected in the second sliding slot and can slide longitudinally along the second sliding slot, the bottom of the second lifting rod is fixedly connected with the second sliding block, the top of the second lifting rod passes through a movable hole adapted to the second sliding slot and then is connected with the bottom surface of the cavity accommodating frame through a lifting head, a return spring is sleeved on the second lifting rod, the return spring is arranged between the top surface of the second sliding block and the top surface of the inner cavity of the second sliding slot, and two ends of the return spring are respectively fixedly connected with the top surface of the second sliding block and the top surface of the inner cavity of the second sliding slot.
4. The parallel plate flow chamber system for simulating a blood flow environment during human movement according to claim 1, wherein a cross-shaped groove is formed in the chamber housing frame, and the size of the groove is adapted to the size of the parallel plate flow chamber.
5. The system of claim 1, wherein the swing mechanism comprises a swing shaft and a swing bearing connected thereto, one end of the swing shaft is connected to the crank-link mechanism, the other end of the swing shaft is connected to the external motor, the swing bearing is disposed in a swing bearing seat, the swing bearing seat comprises a swing upper shaft seat and a swing lower shaft cover, the swing upper shaft seat is fixedly connected to the bottom surface of the connecting bracket, and the swing lower shaft cover is fixedly connected to the swing upper shaft seat.
6. The system of claim 1, wherein the vibration support is fixed to a base plate, the cam rotation mechanism is disposed under the pushing base plate, the cam rotation mechanism comprises a cam, a vibration bearing and a cam shaft, the vibration bearing is connected to a vibration bearing seat, the cam is disposed between the two vibration bearings and is connected to the cam shaft by a key, the vibration bearing is disposed in a bearing cap on the vibration bearing seat, the vibration bearing seat is fixed to the base plate, and a terminal of the cam shaft is connected to an external motor by a key.
7. The system of claim 3, wherein a parking pin hole is formed on the second sliding slot, a parking hole is formed on the second sliding block at a position corresponding to the parking pin hole, and the parking pin sequentially passes through the second sliding slot, the parking pin hole and the parking hole on the second sliding block to realize the parking of the swing module.
8. The parallel plate flow chamber system for simulating a blood flow environment during human body movement according to claim 6, wherein the cam shaft and the vibration support frame are correspondingly provided with pin holes, when the cam reaches a maximum vibration position, the vibration pins are sequentially inserted into the pin holes on the cam shaft and the vibration support frame, and the vibration assembly is fixed in a limiting manner.
9. The parallel plate flow chamber system for simulating a blood flow environment during human body movement according to claim 1, wherein the parallel plate flow chamber comprises an upper perspective plate, an upper parallel plate, a lower parallel plate and a lower perspective plate which are fixedly connected in sequence, the upper perspective plate and the lower perspective plate are both made of transparent materials, a compression gasket is clamped between the upper parallel plate and the lower parallel plate, and rubber gaskets are arranged between the matching surfaces of the upper perspective plate and the upper parallel plate and between the matching surfaces of the lower parallel plate and the lower perspective plate; the upper perspective plate is uniformly provided with a plurality of threaded holes for being in threaded connection with slotted headless axis screws, the positions corresponding to the two sides of the lower parallel plate are respectively provided with a fluid inlet and a fluid outlet for being externally connected with a hollow circular tube, a group of positioning blocks are symmetrically and fixedly arranged in cavities formed by the two ends of the upper parallel plate and the lower parallel plate after being connected, and the outer side ends of the positioning blocks are provided with connecting guide columns.
10. The system of claim 9, wherein three connecting grooves are respectively formed on the four peripheral edges of the cavity receiving frame, the connecting groove at the middle position on the same edge is used for the hollow circular tube to pass through, and the positions of the connecting grooves at the two sides are matched with the positions and sizes of the connecting guide posts on the parallel flat flow cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211150017.6A CN115862442A (en) | 2022-09-21 | 2022-09-21 | Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211150017.6A CN115862442A (en) | 2022-09-21 | 2022-09-21 | Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115862442A true CN115862442A (en) | 2023-03-28 |
Family
ID=85661044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211150017.6A Withdrawn CN115862442A (en) | 2022-09-21 | 2022-09-21 | Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115862442A (en) |
-
2022
- 2022-09-21 CN CN202211150017.6A patent/CN115862442A/en not_active Withdrawn
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20080026465A1 (en) | Cell Culturing Method Using Biomechanical Stimulation Loading And System Therefor | |
| O’Bryan et al. | Three-dimensional printing with sacrificial materials for soft matter manufacturing | |
| US6107081A (en) | Uni-directional cell stretching device | |
| JP4981374B2 (en) | Cell or tissue culture apparatus and culture method | |
| CN103756898B (en) | A kind of stressed culture apparatus of cell three-dimensional applying dynamic load | |
| JP2007534000A (en) | Apparatus and method for stirring a sample during in vitro testing | |
| JP2009511083A (en) | Microfluidic cell culture device and method of use thereof | |
| WO2016041070A1 (en) | Method and apparatus for eye model and testing thereof | |
| CN201268704Y (en) | Dynamic bionic pulsating movement bioreactor | |
| CN206819631U (en) | A kind of teaching apparatus of high analogue simulation human body pupil | |
| CN106867888A (en) | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time | |
| CN115862442A (en) | Parallel flat plate flow cavity system for simulating blood flow environment in human body movement process | |
| CN101906379A (en) | Device for precisely stretching visual cells under simulated in vivo environment | |
| CN108641931A (en) | A kind of digitlization microarray organ chip and its application | |
| CN201883096U (en) | Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor | |
| Liu et al. | High throughput omnidirectional printing of tubular microstructures from elastomeric polymers | |
| US20200339936A1 (en) | Cell Mechanical Stimulating Device | |
| CN201737929U (en) | Precision visualization cell stretching device under environment simulating inner environment of human body | |
| CN113214991B (en) | Cell culture device for simulating cell mechanics microenvironment | |
| DE10151822A1 (en) | Device for the observation of cells and tissues under typical stimulation conditions comprises a culture chamber providing electrical stimulation and mechanical movement, in which the cells are held on a carrier | |
| CN211689016U (en) | Cell carrier stretching device capable of applying circulating stress | |
| CN110438004A (en) | Flat flow chamber that is a kind of while applying static stretch power and Osima jacoti, Osima excavata | |
| EP1990402A1 (en) | Bioreactor to apply mechanical forces as an anabolic stimulus | |
| JP5745589B2 (en) | Cell culture plate with culture equipment | |
| CN110777074A (en) | Multifunctional osteochondral bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20230328 |