CN115851842A - Method for producing hydrogen by utilizing in-situ anaerobic fermentation of microbial oil reservoir - Google Patents
Method for producing hydrogen by utilizing in-situ anaerobic fermentation of microbial oil reservoir Download PDFInfo
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Abstract
The invention discloses: a method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps: (1) screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof; (2) preliminary screening of oil reservoirs; (3) carrying out secondary screening on the oil reservoir by a static culture method; (4) optimizing an injection process; (5) performing site construction; and (6) separating and collecting hydrogen. The method for producing hydrogen by utilizing the in-situ anaerobic fermentation of the microbial oil reservoir has the following beneficial effects: (1) The oil reservoir can provide constant temperature and low oxidation-reduction potential for the anaerobic hydrogen-producing bacteria, and can keep high-speed hydrogen-producing reaction; (2) The hydrogen production cost is greatly reduced by utilizing the original injection and extraction equipment of the near-waste oil reservoir or the waste oil reservoir; (3) Will be provided withThe hydrogen production reaction is transferred from the ground to an underground oil reservoir for carrying out, so that the safety is good; (4) Has the characteristics of simple process, strong operability and good economic benefit, and the hydrogen production cost of the field test is less than 1 yuan/m 3 。
Description
Technical Field
The invention belongs to the technical field of oilfield development, and particularly relates to a method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir.
Background
Hydrogen is an ideal clean energy source, and has high heat value, high heat conversion rate and high energy density. The hydrogen energy is developed vigorously, and the carbon peak reaching and carbon neutralization can be realized with the aid of the power.
The hydrogen production technology mainly comprises a physicochemical method and a biological method, and the biological hydrogen production mainly comprises photosynthetic hydrogen production and anaerobic fermentation hydrogen production. Compared with the anaerobic fermentation hydrogen production, the method has more development potential and is a more ideal hydrogen production method.
The hydrogen production by anaerobic fermentation is that the hydrogen-producing zymocyte produces molecular hydrogen in the process of gradually decomposing organic substrate by fermentation according to the physiological metabolism characteristic of the hydrogen-producing zymocyte, and has the characteristics of good hydrogen production stability, high hydrogen production capacity, high growth rate of the zymocyte and the like. How to design a reaction system suitable for industrialized hydrogen production is the key of anaerobic fermentation hydrogen production.
At present, most of anaerobic fermentation hydrogen production is in laboratory research and pilot-scale test stages, and microbial fermentation equipment or wastewater biochemical treatment equipment such as a continuous flow stirred tank reactor, an upflow anaerobic sludge blanket reactor and the like are mainly adopted as adopted hydrogen production equipment, but the anaerobic fermentation hydrogen production equipment and the wastewater biochemical treatment equipment have the defects of complex structure, unstable operation, low mass transfer efficiency, high operation cost, unsuitability for large-scale industrial production and the like.
Chinese invention patent CN 103725606B discloses a method for using a sequencing batch biological hydrogen production reactor, which relates to a method for using a sequencing batch biological hydrogen production reactor. The method aims to solve the problems that when lignocellulose saccharification liquid is used as a substrate in the conventional biological hydrogen production reactor, the utilization rate of a fermentation hydrogen production substrate is low, and the biomass is easy to lose. The using method comprises the following steps: adding hydrogen-producing bacteria liquid fermentation culture medium into the reactor main body, introducing nitrogen, sealing, and inoculating fermented hydrogen-producing seed liquid for hydrogen-producing fermentation.
Chinese invention patent CN 100491270C discloses a hydrogen production device using high-concentration organic wastewater and a hydrogen production method thereof, which solves the problems of complex structure, unstable operation, low mass transfer efficiency, low biological holding capacity and low impact load resistance of the traditional hydrogen production device. The invention adopts hydrogen production equipment and the following methods: 1. culturing and domesticating anaerobic activated sludge; 2. putting cultured and domesticated anaerobic activated sludge and a light filler (24) into hydrogen production equipment; 3. the temperature is controlled to be 35 +/-3 ℃, and the retention time of water in the equipment is 1.5-6 hours; 4. the high concentration organic wastewater is used for generating hydrogen by anaerobic fermentation of the fermentation substrate and the activated sludge in the reaction zone (22).
However, the technical scheme still has the defects of relatively complex equipment, large one-time investment, high operating cost and the like, for example, nitrogen gas is needed to be introduced and sealed in order to ensure the anaerobic property, accurate temperature control and stirring are needed, and the energy and power consumption is increased. Meanwhile, the hydrogen is produced by fermentation on the ground, and higher requirements are put forward on the safety of equipment and personnel operation.
Disclosure of Invention
The purpose of the invention is as follows: in order to overcome the defects of the prior art, the invention provides a method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir. The method utilizes original injection and extraction equipment of the near-waste oil reservoir and the waste oil reservoir to inject the anaerobic hydrogen production into the oil reservoir, provides constant temperature, proper metal ions and lower oxidation-reduction potential for the anaerobic hydrogen-producing bacteria, and can timely transfer the hydrogen produced in the system, thereby relieving a feedback mechanism formed by high-concentration hydrogen on the hydrogen production reaction and maintaining the high-speed hydrogen production reaction. Meanwhile, hydrogen is produced by in-situ fermentation of the oil reservoir, no complex equipment is additionally added, the cost is low, and the safety is good.
The technical scheme is as follows: a method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
(2) Primary screening of oil reservoirs:
preliminarily screening the oil reservoir by using geological parameters and the requirement of the anaerobic fermentation hydrogen-producing bacteria determined in the step (1) on the temperature;
(3) Carrying out secondary screening on the oil reservoir by a static culture method;
(4) Optimizing an injection process:
optimizing the injection amount and injection concentration of the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production amount;
(5) And (3) field construction:
injecting hydrogen-producing bacteria produced by anaerobic fermentation and a substrate thereof into the target oil reservoir interval through a water injection well pipe column by using the existing water injection system according to the optimized injection process obtained in the step (4);
(6) And (5) separating and collecting hydrogen.
Further, the hydrogen-producing bacteria produced by anaerobic fermentation in the step (1) is one of clostridium butyricum, clostridium pasteurianum, clostridium kloni, clostridium beijerinckii, clostridium acetobutylicum, desulfurization bacteria, enterobacter cloacae, clostridium thermophilum and thermoprobia neoapsi.
Furthermore, the hydrogen-producing bacteria produced by anaerobic fermentation in the step (1) is one of clostridium butyricum, thiobacillus, clostridium thermophilum and thermatopax neapolis.
Further, the substrate in the step (1) is one of glucose, sucrose, hydrolysate of corn starch, molasses wastewater, starch processing wastewater, sugar mill wastewater, leather wastewater and dairy wastewater.
Further, the substrate in the step (1) is one of starch processing wastewater, sugar mill wastewater and dairy plant wastewater.
Further, the preliminary screening criteria for the oil reservoir in step (2) include:
the oil reservoir temperature is within the proper growth temperature range of the anaerobic fermentation hydrogen-producing bacteria determined in the step (1);
500×10 -3 μm 2 oil reservoir permeability is less than or equal to 3000 multiplied by 10 -3 μm 2 ；
The water content of the produced liquid is more than or equal to 95 percent;
the fracture system is clear, and no open fault exists;
a stable compact lithologic interlayer with the thickness more than 10m is arranged at the upper part of the target layer;
the number of the small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m.
Further, the secondary screening of the oil reservoir in the step (3) comprises the following specific steps:
(31) Respectively taking a proper amount of output liquid from each oil reservoir primarily screened in the step (2), and adding the anaerobic fermentation hydrogen-producing bacteria and substrates thereof;
(32) Respectively culturing for 3-5 days at the oil reservoir temperature;
(33) Respectively measuring the bacterial concentrations of the anaerobic fermentation hydrogen-producing bacteria in the culture solution, and screening out 2-3 oil reservoirs corresponding to higher bacterial concentrations according to the bacterial concentrations.
Further, the implantation process optimization in step (4) specifically comprises the following steps:
(41) Washing oil from a natural core of a target oil reservoir to prepare a standard core with phi 25 multiplied by 100 mm;
(42) Vacuumizing, and then saturating formation water of a target oil reservoir;
(43) Saturating the dehydrated and degassed crude oil in the target reservoir;
(44) Water flooding of a target oil reservoir stratum until the water content of produced liquid reaches more than 95%;
(45) Injecting hydrogen-producing bacteria of anaerobic fermentation and substrates thereof with different injection quantities and injection concentration combinations, and statically culturing for 5-10 days;
(46) And measuring the amount of the produced hydrogen, and screening the injection process corresponding to the core with the maximum hydrogen production amount as an optimized injection process.
Further, the step (5) comprises the following steps:
(51) Ground mixing, preparing and pressurizing injection equipment, wherein the pressurizing injection equipment comprises two stirring pools;
(52) Mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof in stirring tanks, alternately preparing the two stirring tanks, pressurizing by a plunger pump, adopting an electromagnetic flowmeter for measuring the injection amount, and automatically adjusting and injecting the injection concentration and the injection amount by automatic control equipment;
(53) Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
Further, the step (6) comprises the following steps:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment.
Further, the step (6) includes the steps of:
the anaerobic fermentation hydrogen-producing bacteria are used for producing hydrogen in the propagation of an oil reservoir, after the hydrogen-producing starting time is reached, the oil well is started to be pumped, liquid is pumped to the ground through an oil pipe by using a gas-liquid separation pipe column of the oil well, gas enters a sleeve through a channel of a gas-liquid separator and is input to the ground, and hydrogen and produced liquid are preliminarily separated in the oil well pipe column;
after the produced gas reaches the ground, the gas-liquid secondary separation, purification and collection are carried out by utilizing equipment processes such as a ground oil-gas separator and the like.
The core component of the gas-liquid separation column is a liquid-gas pump: the air storage cylinder is arranged in the middle of the pump body, and has the functions of gas distribution and gas production, so that the influence of gas is reduced, and the pump efficiency is improved.
The structure of the liquid-air pump: mainly comprises an upper pump joint, a lower pump joint, a pump barrel, a plunger, an oil drain device fixing valve and the like. The pump cylinder of the liquid-gas pump is formed by connecting three sections, namely a long pump cylinder section, an air pocket section, a short pump cylinder section and the like from bottom to top in sequence, the fixed valve, the traveling valve and the pump plunger are the same as those of a conventional oil well pump, and the air pocket section is of a double-layer cavity structure.
In the production suction up stroke, the plunger gives way out of the pump space, and the liquid enters the pump barrel; the plunger continues to move upwards, and when the top dead center is reached, the lower end of the plunger enters the air storage cylinder by 1/2, so that gas-liquid separation is realized; in the down stroke, the upper end of the plunger enters the air storage cylinder, gas is released and enters the upper pump cylinder or the oil pipe, the fixed valve is closed, the traveling valve is rapidly opened, and liquid enters the piston.
The liquid is pumped to the ground through an oil pipe, the gas enters the sleeve through a channel of the gas-liquid separator and is input to the ground, the hydrogen and the produced liquid are primarily separated, and after the gas is produced to the ground, the gas-liquid secondary separation and purification are carried out by using a ground oil-gas separator.
Has the beneficial effects that: compared with the prior art, the method for producing hydrogen by utilizing the in-situ anaerobic fermentation of the microbial oil reservoir has the following advantages:
(1) The oil reservoir can provide constant temperature and low oxidation-reduction potential for the anaerobic hydrogen-producing bacteria, and can keep high-speed hydrogen-producing reaction;
(2) The original injection and extraction equipment of the near-waste oil reservoir or the waste oil reservoir is utilized, no complex equipment is additionally added, and the hydrogen production cost is greatly reduced;
(3) The hydrogen production reaction is transferred from the ground to the underground oil reservoir for carrying out, so that the safety is good;
(4) Has the characteristics of simple process, strong operability and good economic benefit, and the hydrogen production cost of the field test is less than 1 yuan/m 3 。
The specific implementation mode is as follows:
the following is a detailed description of specific embodiments of the invention.
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
According to the purpose of the invention, the invention discloses a method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir, which comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
(2) Primary screening of oil reservoirs:
preliminarily screening the oil reservoir by using geological parameters and the requirement of the hydrogen-producing anaerobic fermentation bacteria determined in the step (1) on the temperature;
(3) Carrying out secondary screening on the oil reservoir by a static culture method;
(4) Optimizing an injection process:
optimizing the injection amount and the injection concentration of the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof, wherein the optimization method adopts a physical simulation method and the optimization basis is the hydrogen-producing gas amount;
(5) And (3) field construction:
injecting hydrogen-producing bacteria produced by anaerobic fermentation and a substrate thereof into the target oil reservoir interval through a water injection well pipe column by using the existing water injection system according to the optimized injection process obtained in the step (4);
(6) And (5) separating and collecting hydrogen.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention can be made, and the same should be considered as the disclosure of the present invention as long as the idea of the present invention is not violated.
The present invention will be further described with reference to specific examples.
Example 1:
a method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the anaerobic fermentation hydrogen-producing bacteria is clostridium butyricum, and the substrate is dairy waste water; the selected clostridium butyricum strictly grows in an anaerobic way, the optimal growth temperature is 35 ℃, and the proper pH value is 6-8;
(2) Primary screening of oil reservoirs: the proper temperature of the strain is 35 ℃, the proper pH value is 6-8, and the strain combines the requirements of proper oil reservoir parameters: the Tibetan permeability is 500-3000 multiplied by 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable compact lithologic interlayer with the thickness of more than 10m to screen suitable blocks. The number of small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m. A total of 5 conforming blocks are screened.
TABLE 1 preliminary screening of suitable reservoirs for hydrogen production by Clostridium butyricum
(3) Secondary screening of oil reservoirs by a static culture method:
the specific steps of oil reservoir screening are as follows: adding 80mL of output liquid of the oil reservoirs A1-A5 into a 100mL conical flask, and adding 2% of clostridium butyricum and 20% of dairy plant wastewater; then culturing the seeds at 35 ℃ for 10 days at the oil reservoir temperature; and (3) determining the bacterial concentration of the clostridium butyricum in the culture solution, wherein the determination result is shown in table 1, and preliminarily screening out the oil reservoir with the highest bacterial concentration according to the bacterial concentration.
TABLE 2 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
| Serial number | Bacterial concentration, one/ml | Sorting |
| Reservoir A1 | 9.0×10 7 | 3 |
| Oil pool A2 | 5.0×10 7 | 4 |
| Oil reservoir A3 | 4.0×10 7 | 5 |
| Reservoir A4 | 5.0×10 8 | 1 |
| Reservoir A5 | 4.0×10 8 | 2 |
As can be seen from Table 2, the anaerobic fermentation hydrogen-producing bacterium Clostridium butyricum activated in the oil reservoir A4 has the highest concentration. The effective thickness of A4 oil deposit is 8m, the number of small layers is 3, the dip angle of the stratum is 1-2 degrees, 1 injection is 4 mining, and the pore volume is 28.8X 10 4 m 3 。
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir A4 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate formation water of an oil reservoir A4; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of produced fluid reaches 95%. Then, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof with different injection amounts and injection concentration combinations are injected, and the results are shown in Table 3. Static culture for 10d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 3 injection concentration optimization
TABLE 4 Implantation optimization
Through the physical model experiment, the injection concentration is determined to be 20%, and the injection amount is determined to be 0.3PV. The daily injection amount of the water well is kept unchanged, and a tank truck is adopted for periodic injection in order to reduce the cost and combine the production characteristics of microorganisms. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing preparation pressurization injection equipment: the pressurizing injection equipment comprises two 20-square stirring tanks, wherein the stirring tanks are provided with stirrers controlled by variable frequency motors, and the rotating speed is 30r/min; mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof in stirring tanks, alternately preparing the hydrogen-producing bacteria and the substrates in the two stirring tanks, and pressurizing by a plunger pump until the rated discharge capacity is 15m 3 H, rated pressure 25 MPa), injection quantity 10m 3 The measurement is carried out by an electromagnetic flowmeter (0.5-16 m) 3 H) of the reaction. Parameters such as injection concentration and injection amount are automatically adjusted through automatic control equipment, injection precision is improved, and injection quality is guaranteed. Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in oil reservoirs, and are separated and collected through the oil well production string and ground equipment.
The method comprises the steps of breeding microorganisms in an oil reservoir to produce hydrogen, pumping an oil well after the hydrogen production starting time is reached, pumping liquid to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 70 pump) of the oil well, enabling gas to enter a sleeve through a channel of a gas-liquid separator, inputting the gas to the ground, carrying out primary separation on hydrogen and produced liquid in the oil well pipe column, and carrying out secondary gas-liquid separation, purification and collection by using equipment processes such as a ground oil-gas separator after the produced gas reaches the ground.
And (3) field test result evaluation: and after the A4 oil reservoir is filled for 6 months and 30 days in 2021, 10 ten thousand of bacteria liquid and nutrient systems are injected accumulatively, the injection cost is about 5 yuan/square, the hydrogen production is 150 ten thousand, the sewage treatment is omitted, the input-output ratio is greater than 1.
Example 2
A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the anaerobic fermentation hydrogen-producing bacteria are desulfurization bacteria, and the substrate is starch processing wastewater. The selected desulfurization bacteria strictly grow anaerobically, the optimal growth temperature is 50 ℃, and the proper pH value is 5-7;
(2) Primary screening of oil reservoirs:
the proper temperature of the strain is 50 ℃, the proper pH value is 5-7, and the strain combines the requirements of proper oil reservoir parameters: the Tibetan permeability is 500-3000 multiplied by 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable dense lithologic interlayer with the thickness more than 10m to screen a proper block. The number of small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m. A total of 4 conforming blocks are screened.
TABLE 5 preliminary screening of hydrogen-producing suitable reservoirs for desulfurization bacteria
(3) The method for screening the oil reservoirs adopts an anaerobic fermentation hydrogen-producing bacteria static culture method, and the screening basis is the bacterial concentration of the anaerobic fermentation hydrogen-producing bacteria.
The specific steps of oil reservoir screening are as follows: taking 100mL of a conical flask, adding 90mL of output liquid of the oil reservoirs B1-B4, and adding 2% of desulfurization bacteria and 20% of starch processing wastewater; then placing the mixture at 50 ℃ under the oil reservoir temperature for culturing for 10d; and (3) determining the bacterial concentration of the desulfurization bacteria in the culture solution, wherein the determination result is shown in table 6, and preliminarily screening out the oil reservoir with the highest bacterial concentration according to the bacterial concentration.
TABLE 6 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
| Serial number | Bacteria concentration, one/ml | Sorting |
| Reservoir B1 | 6.0×10 7 | 3 |
| Reservoir B2 | 4.0×10 8 | 2 |
| Reservoir B3 | 2.0×10 8 | 4 |
| Reservoir B4 | 5.0×10 8 | 1 |
As can be seen from Table 6, the activated desulfurization bacteria were most concentrated in reservoir B4. Wherein the effective thickness of the B4 oil reservoir is 18m, the number of small layers is 2, the dip angle of the stratum is 1-3 degrees, 2-injection and 5-extraction are carried out, and the pore volume is 46.3 x 10 4 m 3 This time, the B4 implementation is preferred.
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir B4 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate reservoir B4 formation water; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of produced fluid reaches 95%. Then, hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof were injected at different injection rates and injection concentration combinations, as shown in Table 7. Static culture for 5d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 7 implant concentration optimization
TABLE 8 injection optimization
Through a physical model experiment, the injection concentration is determined to be 15%, and the injection amount is determined to be 0.3PV. The daily injection amount of the water well is kept unchanged, and a tank truck is adopted for periodic injection in order to reduce the cost and combine the production characteristics of microorganisms. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing preparation pressurization injection equipment: the pressurizing injection equipment comprises two 30-square stirring tanks, wherein the stirring tanks are provided with stirrers controlled by variable frequency motors, and the rotating speed is 35r/min; producing hydrogen by anaerobic fermentationMixing and stirring the bacteria and the substrate thereof in a stirring pool, alternately preparing the bacteria and the substrate in the two stirring pools, and pressurizing by a plunger pump until the rated discharge capacity is 15m 3 H, rated pressure 20 MPa), injection amount 12m 3 The metering adopts an electromagnetic flowmeter (0.5-16 m) 3 H). Parameters such as injection concentration and injection amount are automatically adjusted through automatic control equipment, injection precision is improved, and injection quality is guaranteed. Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment. The method comprises the steps of breeding microorganisms in an oil reservoir to produce hydrogen, pumping an oil well after the hydrogen production starting time is reached, pumping liquid to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 83 pump) of the oil well, enabling gas to enter a sleeve through a channel of a gas-liquid separator, inputting the gas to the ground, primarily separating hydrogen and produced liquid in the oil well pipe column, and performing gas-liquid secondary separation, purification and collection by using equipment processes such as a ground oil-gas separator after the produced gas reaches the ground.
And (3) evaluating field test results: and after the oil reservoir B4 is filled for 6 months and 30 days in 2021, the bacteria liquid and nutrient system are injected accumulatively for 12 ten thousand formulas, the injection cost is about 5 yuan/formula, the hydrogen is produced for 168 ten thousand formulas, the sewage treatment is omitted, the input-output ratio is 1.97, the field effect is good, and the invention has wide popularization and application prospects.
Example 3
A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the anaerobic fermentation hydrogen-producing bacteria is clostridium thermophilum, and the substrate is wastewater of a dairy factory. The selected clostridium thermophilum strictly grows anaerobically, the optimal growth temperature is 60 ℃, and the proper pH value is 7-8;
(2) Primary screening of oil reservoirs: the proper temperature of the strain is 60 ℃, the proper pH value is 7-8, and the strain combines the proper oil reservoir parameter requirements: the storage permeability is 500-3000 x 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable compact lithologic interlayer with the thickness of more than 10m to screen suitable blocks. The number of small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m. A total of 4 conforming blocks are screened.
TABLE 9 preliminary screening of suitable reservoirs for hydrogen production by clostridium thermophilum
(3) And (3) secondary screening of the oil reservoir, wherein the screening method adopts an anaerobic fermentation hydrogen-producing bacteria static culture method, and the screening basis is the bacterial concentration of the anaerobic fermentation hydrogen-producing bacteria.
The specific steps of oil reservoir screening are as follows: adding 85mL of oil deposit C1-C4 output liquid into a 100mL conical flask, and adding 2% of clostridium thermophilum and 20% of dairy waste water; then placing the mixture at the oil reservoir temperature of 60 ℃ for culturing for 10d; and (3) determining the bacterial concentration of the clostridium thermophilum in the culture solution, wherein the determination result is shown in a table 10, and preliminarily screening out the oil reservoir with the highest bacterial concentration according to the bacterial concentration.
TABLE 10 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
As can be seen from table 6, the activated clostridium thermophilum strain is the most concentrated at reservoir C2. Wherein the effective thickness of the C2 oil reservoir is 13m, the number of small layers is 4, the dip angle of the stratum is 1-2 degrees, 2-injection 4-production and the pore volume is 42.6 x 10 4 m3, this time the C2 implementation is preferred.
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir C2 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate C2 formation water of the oil reservoir; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of produced fluid reaches 95%. Then, the anaerobically fermented hydrogenogen and its substrate were injected at different injection quantities and injection concentration combinations, as shown in table 11. Static culture for 8d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 11 implant concentration optimization
TABLE 12 injection optimization
Through a physical model experiment, the injection concentration is determined to be 20%, and the injection amount is determined to be 0.2PV. The daily injection amount of the water well is kept unchanged, and a tank truck is adopted for periodic injection in order to reduce the cost and combine the production characteristics of microorganisms. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing and preparing pressurized injection equipment, wherein the pressurized injection equipment comprises two 20-square stirring tanks, and the stirring tanks are provided with stirrers controlled by variable frequency motors and rotate at the speed of 35r/min; mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrate thereof in a stirring tank, preparing the hydrogen-producing bacteria and the substrate in turn in the two stirring tanks, and pressurizing by a plunger pump to 15m of rated discharge capacity 3 H, rated pressure 20 MPa), injection amount 9m 3 The metering adopts an electromagnetic flowmeter (0.5-12 m) 3 H) of the reaction. Parameters such as injection concentration and injection amount are automatically adjusted through automatic control equipment, injection precision is improved, and injection quality is guaranteed. Injecting a water well into the pipe column: according to the designed concentration and injection, the hydrogen-producing bacteria produced by anaerobic fermentationAnd injecting the substrate into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment. The method comprises the steps of breeding microorganisms in an oil reservoir to produce hydrogen, pumping an oil well after the hydrogen production starting time is reached, pumping liquid to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 70 pump) of the oil well, enabling gas to enter a sleeve through a channel of a gas-liquid separator, inputting the gas to the ground, carrying out primary separation on hydrogen and produced liquid in the oil well pipe column, and carrying out secondary gas-liquid separation, purification and collection by using equipment processes such as a ground oil-gas separator after the produced gas reaches the ground.
And (3) field test result evaluation: and 6, 30 days after 2021, 8.1 million bacteria liquid and nutrient system are injected accumulatively until the C2 oil reservoir is subjected to 6 months and 30 days, the injection cost is about 5 yuan/square, 112 million hydrogen is produced, sewage treatment is omitted, the input-output ratio is 1.01, the field effect is good, and the method has wide popularization and application prospects.
Example 4
A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the hydrogen-producing bacteria by anaerobic fermentation is new Apollo Thermotoga, and the substrate is waste water of sugar refinery. The selected Thermotoga neoporioides grows strictly anaerobically at the optimal growth temperature of 70 ℃ and the proper pH value of 6-8.
(2) Primary screening of oil reservoirs: the proper temperature of the strain is 70 ℃, the proper pH value is 6-8, and the strain combines the proper oil reservoir parameter requirements: the Tibetan permeability is 500-3000 multiplied by 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable dense lithologic interlayer with the thickness more than 10m to screen a proper block. The number of small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m. A total of 5 conforming blocks are screened.
TABLE 13 preliminary screening of suitable reservoirs for hydrogen production by Thermotoga neobola
(3) And (3) secondary screening of the oil reservoir, wherein the screening method adopts an anaerobic fermentation hydrogen-producing bacteria static culture method, and the screening basis is the bacterial concentration of the anaerobic fermentation hydrogen-producing bacteria.
The specific steps of oil reservoir screening are as follows: adding 80mL of oil deposit D1-D5 production liquid into a 100mL conical flask, and adding 2% of Thermotoga neoporiosus and 20% of sugar refinery wastewater; then placing the mixture at 70 ℃ under the oil reservoir temperature for culturing for 10d; the bacterial concentration of the new Apollo Thermotoga in the culture solution is determined, the determination result is shown in Table 14, and the oil reservoir with the highest bacterial concentration is preliminarily screened according to the bacterial concentration.
TABLE 14 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
| Serial number | Bacteria concentration, one/mL | Sorting |
| Oil reservoir D1 | 3.0×10 7 | 4 |
| Reservoir D2 | 3.0×10 8 | 2 |
| Reservoir D3 | 8.0×10 8 | 1 |
| Reservoir D4 | 5.0×10 7 | 3 |
| Reservoir D5 | 3.0×10 6 | 5 |
As can be seen from Table 14, the activated Thermotoga neoporiosus was most concentrated in reservoir D3. Wherein the effective thickness of the D3 oil reservoir is 6m, the number of small layers is 1, the dip angle of the stratum is 1-2 degrees, 1 injection is 3 mining, and the pore volume is 29.6 × 104m 3 This time, D3 implementation is preferred.
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir D3 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate reservoir D3 formation water; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of produced fluid reaches 95%. Then, the hydrogen-producing bacteria and the substrate for anaerobic fermentation were injected at different injection quantities and injection concentration combinations, as shown in Table 15. Static culture for 6d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 15 implant concentration optimization
TABLE 16 Implantation optimization
Through a physical model experiment, the injection concentration is determined to be 15%, and the injection amount is determined to be 0.2PV. The daily injection amount of the water well is kept unchanged, and in order to reduce the cost and combine the production characteristics of microorganisms, a tank car is adopted for periodic injection. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing preparation pressurization injection equipment: the pressurizing injection equipment comprises two 40-square stirring tanks, wherein the stirring tanks are provided with stirrers controlled by variable frequency motors, and the rotating speed is 30r/min; mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof in stirring tanks, alternately preparing the hydrogen-producing bacteria and the substrates in the two stirring tanks, and pressurizing by a plunger pump until the rated discharge capacity is 15m 3 H, rated pressure 20 MPa), injection quantity 10m 3 The metering adopts an electromagnetic flowmeter (0.5-15 m) 3 H) of the reaction. Parameters such as injection concentration and injection amount are automatically adjusted through automatic control equipment, injection precision is improved, and injection quality is guaranteed. Injecting a water well into a pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment.
The anaerobic fermentation hydrogen-producing bacteria are used for producing hydrogen in the propagation of an oil reservoir, after the hydrogen-producing pumping time is reached, the oil well is pumped, liquid is pumped to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 83 pump) of the oil well, gas enters a sleeve through a channel of a gas-liquid separator and is input to the ground, the hydrogen and produced liquid are preliminarily separated in the oil well pipe column, and after the produced gas reaches the ground, the gas-liquid secondary separation, purification and collection are carried out by using equipment flows such as a ground oil-gas separator and the like.
And (3) field test result evaluation: and D3, accumulating injected bacterial liquid and a nutrient system for 5.1 ten thousand in each square after 6 months and 30 days in 2021, wherein the injection cost is about 5 yuan per square, the hydrogen production is 59 ten thousand in each square, the sewage treatment is omitted, the input-output ratio is 1.7, the field effect is good, and the method has wide popularization and application prospects.
Example 5
A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the anaerobic fermentation hydrogen-producing bacteria is clostridium pasteurianum, and the substrate is glucose. The selected clostridium pasteurianum strictly grows anaerobically, the optimal growth temperature is 45 ℃, and the proper pH value is 7-9.
(2) Primary screening of oil reservoirs: the proper temperature of the strain is 45 ℃, the proper pH value is 7-9, and the strain combines the proper oil reservoir parameter requirements: the Tibetan permeability is 500-3000 multiplied by 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable compact lithologic interlayer with the thickness of more than 10m to screen suitable blocks. The number of the small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m. A total of 5 conforming blocks are screened.
TABLE 17 preliminary screening of suitable reservoirs for hydrogen production by Clostridium pasteurianum
(3) And (3) secondary screening of the oil reservoir, wherein the screening method adopts an anaerobic fermentation hydrogen-producing bacteria static culture method, and the screening basis is the bacterial concentration of the anaerobic fermentation hydrogen-producing bacteria.
The specific steps of oil reservoir screening are as follows: adding 80mL of oil deposit E1-E5 output liquid into a 100mL conical flask, and adding 2% of clostridium pasteurianum and 20% of glucose; then culturing the seeds at 45 ℃ for 10 days at the oil reservoir temperature; and (3) determining the bacterial concentration of the clostridium pasteurianum in the culture solution, wherein the determination result is shown in a table 14, and preliminarily screening out the oil reservoir with the highest bacterial concentration according to the bacterial concentration.
TABLE 18 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
| Serial number | Bacterial concentration, count/mL | Sorting |
| Reservoir E1 | 5.1×10 7 | 3 |
| Reservoir E2 | 3.8×10 8 | 2 |
| Oil reservoir E3 | 8.1×10 8 | 1 |
| Oil reservoir E4 | 3.2×10 7 | 4 |
| Reservoir E5 | 3.6×10 6 | 5 |
As can be seen from table 14, clostridium pasteurianum activated at reservoir E3 is the highest. Wherein the effective thickness of the E3 oil reservoir is 7m, the number of small layers is 2, the dip angle of the stratum is 1-3 degrees, 1 injection is 3 mining, and the pore volume is 23.52 multiplied by 104m 3 This time, E3 implementation is preferred.
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir E3 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate reservoir E3 stratum water; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of produced fluid reaches 95%. Then, the hydrogen-producing bacteria and the substrate for anaerobic fermentation were injected at different injection quantities and injection concentration combinations, as shown in Table 15. Static culture for 6d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 19 implant concentration optimization
TABLE 20 Implantation optimization
Through the physical model experiment, the injection concentration is determined to be 20%, and the injection amount is determined to be 0.3PV. The daily injection amount of the water well is kept unchanged, and in order to reduce the cost and combine the production characteristics of microorganisms, a tank car is adopted for periodic injection. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing preparation pressurization injection equipment: the pressurizing injection equipment comprises two 40-square stirring tanks, wherein the stirring tanks are provided with stirrers controlled by variable frequency motors, and the rotating speed is 30r/min; mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof in stirring tanks, alternately preparing the hydrogen-producing bacteria and the substrates in the two stirring tanks, and pressurizing by a plunger pump until the rated discharge capacity is 15m 3 H, rated pressure 20 MPa), injection amount 10m 3 The measurement adopts an electromagnetic flowmeter (0.5 to 15 m) 3 H). Parameters such as injection concentration and injection amount are automatically adjusted through automatic control equipment, injection precision is improved, and injection quality is guaranteed. Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment.
The anaerobic fermentation hydrogen-producing bacteria are used for producing hydrogen in the propagation of an oil reservoir, after the hydrogen-producing starting time is reached, the oil well is started to be pumped, liquid is pumped to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 83 pump) of the oil well, gas enters a sleeve through a channel of a gas-liquid separator and is input to the ground, the hydrogen and the produced liquid are preliminarily separated in the oil well pipe column, and after the gas is produced to the ground, the gas-liquid secondary separation, purification and collection are carried out by using equipment flows such as a ground oil-gas separator and the like.
And (3) evaluating field test results: and 4.3 ten thousand of bacteria liquid and nutrient system are injected accumulatively until 2021, 6 months and 30 days, the injection cost is about 5 yuan/square, the hydrogen production is 46 ten thousand square, the sewage treatment is omitted, the input-output ratio is 1.6, the field effect is good, and the invention has wide popularization and application prospect.
Example 6
A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir comprises the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
the hydrogen-producing bacteria by anaerobic fermentation is clostridium kluyveri, and the substrate is glucose. The selected Clostridium kluyveri grows strictly anaerobically, the optimal growth temperature is 60 ℃, and the proper pH value is 5-7.
(2) Primary screening of oil reservoirs: the strain is suitable for 60 ℃ and 5-7 of pH, and combines the requirements of suitable oil reservoir parameters: the storage permeability is 500-3000 x 10 -3 μm 2 Within the range; the water content of the produced liquid is more than or equal to 95 percent; the fracture system is clear, and open faults do not exist; the upper part of the target layer is provided with a stable compact lithologic interlayer with the thickness of more than 10m to screen suitable blocks. The number of small layers of the target layer is not more than 5, and the thickness of the small layer is more than 2m. A total of 4 conforming blocks are screened.
TABLE 21 preliminary screening of suitable reservoirs for hydrogen production by Clostridium kluyveri
(3) And (3) secondary screening of the oil reservoir, wherein the screening method adopts an anaerobic hydrogen-producing fermentation static culture method, and the screening basis is the bacterial concentration of the anaerobic hydrogen-producing fermentation bacteria.
The specific steps of oil reservoir screening are as follows: adding 80mL of output liquid of oil reservoirs E1-E5 into a 100mL conical flask, and adding 2% of clostridium kluyveri and 20% of glucose; then placing the mixture at the oil reservoir temperature of 60 ℃ for culturing for 10d; and (3) determining the bacterial concentration of the clostridium kluyveri in the culture solution, wherein the determination result is shown in a table 14, and preliminarily screening out the oil reservoir with the highest bacterial concentration according to the bacterial concentration.
TABLE 22 results of anaerobic fermentation hydrogen-producing bacteria concentration and sequencing after activation of different oil reservoirs
| Serial number | Bacterial concentration, count/mL | Sorting |
| Oil reservoir F1 | 5.0×10 7 | 3 |
| Reservoir F2 | 7.8×10 8 | 1 |
| Oil reservoir F3 | 4.0×10 8 | 2 |
| Reservoir F4 | 3.0×10 7 | 4 |
As can be seen from table 14, clostridium kloni activated at reservoir F2 is the highest concentrated. Wherein the effective thickness of the F2 oil reservoir is 10m, the number of small layers is 3, and the dip angle of the stratum is 1-22 samples at 1 st, pore volume 32.4 x 10 4 m 3 This time, F2 implementation is preferred.
(4) And optimizing the injection process, wherein the optimization method adopts a physical simulation method and the optimization basis is hydrogen production.
Firstly, washing oil from a natural core of an oil reservoir F2 to prepare a standard core with phi 25 multiplied by 100 mm; vacuumizing to saturate reservoir F2 formation water; dehydrating and degassing crude oil of a saturated target oil reservoir; and (4) driving the formation water until the water content of the produced fluid reaches 95 percent. Then, the anaerobic fermentation hydrogen-producing bacteria and substrates thereof with different injection amounts, injection concentrations and injection period combinations are injected, as shown in Table 15. Static culture for 6d; then measuring the amount of the produced hydrogen; and finally, screening the injection process corresponding to the core with the largest hydrogen production rate as an optimized injection process.
TABLE 23 injection concentration optimization
TABLE 24 Implantation optimization
Through a physical model experiment, the injection concentration is determined to be 15%, and the injection amount is determined to be 0.3PV. The daily injection amount of the water well is kept unchanged, and a tank truck is adopted for periodic injection in order to reduce the cost and combine the production characteristics of microorganisms. Injecting bacteria liquid and nutrient solution 3-5 days in each month, and injecting water in the rest time.
(5) And (3) field construction:
ground mixing preparation pressurization injection equipment: the pressurizing injection equipment comprises two 40-square stirring tanks, wherein the stirring tanks are provided with stirrers controlled by variable frequency motors, and the rotating speed is 30r/min; mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrate thereof in a stirring tank, preparing the hydrogen-producing bacteria and the substrate in turn in the two stirring tanks, and pressurizing by a plunger pump to 15m of rated discharge capacity 3 H, rated pressure 20 MPa), injection quantity 10m 3 The metering adopts an electromagnetic flowmeter (0.5-15 m) 3 H). The parameters such as the injection concentration and the injection quantity are automatically controlledThe chemical control equipment carries out automatic adjustment, improves the injection precision and ensures the injection quality. Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
(6) Separating and collecting hydrogen:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment.
The anaerobic fermentation hydrogen-producing bacteria are used for producing hydrogen in the propagation of an oil reservoir, after the hydrogen-producing pumping time is reached, the oil well is pumped, liquid is pumped to the ground through an oil pipe by using a gas-liquid separation pipe column (phi 83 pump) of the oil well, gas enters a sleeve through a channel of a gas-liquid separator and is input to the ground, the hydrogen and produced liquid are preliminarily separated in the oil well pipe column, and after the produced gas reaches the ground, the gas-liquid secondary separation, purification and collection are carried out by using equipment flows such as a ground oil-gas separator and the like.
And (3) evaluating field test results: and 6, 30 days after 2021, 8.4 million bacteria liquid and nutrient system are injected accumulatively until the F2 oil reservoir is 2021, the injection cost is about 5 yuan/square, 86 million hydrogen is produced, sewage treatment is omitted, the input-output ratio is 1.5, the field effect is good, and the method has wide popularization and application prospects.
The embodiments of the present invention have been described in detail. However, the present invention is not limited to the above-described embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (11)
1. A method for producing hydrogen by utilizing in-situ anaerobic fermentation of a microbial oil reservoir is characterized by comprising the following steps:
(1) Screening hydrogen-producing bacteria by anaerobic fermentation and substrates thereof;
(2) Primary screening of oil reservoirs:
preliminarily screening the oil reservoir by using geological parameters and the requirement of the anaerobic fermentation hydrogen-producing bacteria determined in the step (1) on the temperature;
(3) Carrying out secondary screening on the oil reservoir by a static culture method;
(4) Optimizing an injection process:
optimizing the injection amount and the injection concentration of the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof, wherein the optimization method adopts a physical simulation method and the optimization basis is the hydrogen-producing gas amount;
(5) And (3) field construction:
injecting hydrogen-producing bacteria produced by anaerobic fermentation and a substrate thereof into the target oil reservoir interval through a water injection well pipe column by using the existing water injection system according to the optimized injection process obtained in the step (4);
(6) And (5) separating and collecting hydrogen.
2. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 1, wherein the hydrogen-producing bacteria produced by anaerobic fermentation in step (1) is one of Clostridium butyricum, clostridium pasteurianum, clostridium kloni, clostridium beijerinckii, clostridium acetobutylicum, thiobacillus, enterobacter cloacae, clostridium thermophilum, and Thermotoga neapolis.
3. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 2, wherein the hydrogen-producing bacteria produced by anaerobic fermentation in step (1) is one of Clostridium butyricum, thiobacillus, clostridium thermophilum, thermotoga neoalbopictus.
4. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 1, wherein the substrate in step (1) is one of glucose, sucrose, hydrolysate of corn starch, molasses wastewater, starch processing wastewater, sugar mill wastewater, leather wastewater and dairy wastewater.
5. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 4, wherein the substrate in step (1) is one of starch processing wastewater, sugar mill wastewater and dairy wastewater.
6. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 1, wherein the preliminary screening criteria for the oil reservoir in step (2) comprise:
the oil reservoir temperature is within the proper growth temperature range of the anaerobic fermentation hydrogen-producing bacteria determined in the step (1);
500×10 -3 μm 2 oil reservoir permeability is less than or equal to 3000 multiplied by 10 -3 μm 2 ；
The water content of the produced liquid is more than or equal to 95 percent;
the fracture system is clear, and open faults do not exist;
a stable compact lithologic interlayer with the thickness more than 10m is arranged at the upper part of the target layer;
the number of small layers of the target layer is not more than 6, and the thickness of the small layer is more than 2m.
7. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 1, wherein the secondary screening of the oil reservoir in step (3) comprises the following specific steps:
(31) Respectively taking a proper amount of output liquid from each oil reservoir primarily screened in the step (2), and adding the anaerobic fermentation hydrogen-producing bacteria and substrates thereof;
(32) Respectively culturing for 3-5 days at the oil reservoir temperature;
(33) Respectively measuring the bacterial concentrations of the anaerobic fermentation hydrogen-producing bacteria in the culture solution, and screening out 2-3 oil reservoirs corresponding to higher bacterial concentrations according to the bacterial concentrations.
8. The method for producing hydrogen by in-situ anaerobic fermentation of a microbial oil reservoir as claimed in claim 1, wherein the injection process in step (4) is optimized by the following specific steps:
(41) Washing oil from a natural core of a target oil reservoir to prepare a standard core with phi 25 multiplied by 100 mm;
(42) Vacuumizing, and then saturating formation water of a target oil reservoir;
(43) Saturating the dehydrated and degassed crude oil of the target oil reservoir;
(44) Water flooding of a target oil reservoir stratum until the water content of produced liquid reaches more than 95%;
(45) Injecting hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof with different injection amounts and injection concentration combinations, and statically culturing for 5-10 days;
(46) And measuring the amount of the produced hydrogen, and screening the injection process corresponding to the core with the maximum hydrogen production amount as an optimized injection process.
9. The method for producing hydrogen by in-situ anaerobic fermentation of microbial oil reservoirs according to claim 1, wherein step (5) comprises the following steps:
(51) Ground mixing and preparing a pressurizing injection device, wherein the pressurizing injection device comprises two stirring pools;
(52) Mixing and stirring hydrogen-producing bacteria produced by anaerobic fermentation and substrates thereof in stirring tanks, alternately preparing the two stirring tanks, pressurizing by a plunger pump, adopting an electromagnetic flowmeter for measuring the injection amount, and automatically adjusting and injecting the injection concentration and the injection amount by automatic control equipment;
(53) Injecting a water well into the pipe column: according to the designed concentration and injection allocation, the anaerobic fermentation hydrogen-producing bacteria and the substrate thereof are injected into a target oil layer through a water well pipe column.
10. The method for producing hydrogen by using the in-situ anaerobic fermentation of the microbial oil reservoir as claimed in claim 1, wherein the step (6) comprises the following steps:
the anaerobic fermentation hydrogen-producing bacteria are propagated and produced hydrogen in the oil reservoir, and are separated and collected by the oil well production pipe column and ground equipment.
11. The method for producing hydrogen by in-situ anaerobic fermentation of microbial oil reservoirs according to claim 10, wherein step (6) comprises the following steps:
the anaerobic fermentation hydrogen-producing bacteria are used for producing hydrogen in the propagation of an oil reservoir, after the hydrogen-producing pumping time is reached, the oil well is pumped, liquid is pumped to the ground through an oil pipe by using a gas-liquid separation pipe column of the oil well, gas enters a sleeve through a channel of a gas-liquid separator and is input to the ground, and the hydrogen and produced liquid are primarily separated in the oil well pipe column;
after the produced gas reaches the ground, the gas-liquid secondary separation, purification and collection are carried out by utilizing equipment processes such as a ground oil-gas separator and the like.
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| CN116284568B (en) * | 2023-05-18 | 2023-08-15 | 德仕能源科技集团股份有限公司 | Compound for promoting microorganisms to produce hydrogen by utilizing waste oil reservoirs and preparation method thereof |
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