CN115837290A - Double T-shaped quantitative sampling micro-fluidic chip and clamp applied to chip - Google Patents
Double T-shaped quantitative sampling micro-fluidic chip and clamp applied to chip Download PDFInfo
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- CN115837290A CN115837290A CN202211242696.XA CN202211242696A CN115837290A CN 115837290 A CN115837290 A CN 115837290A CN 202211242696 A CN202211242696 A CN 202211242696A CN 115837290 A CN115837290 A CN 115837290A
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Abstract
The invention relates to the technical field of microfluidics, in particular to a double T-shaped quantitative sampling microfluidic chip and a clamp applied to the chip, which comprises a quantitative channel, wherein a liquid inlet channel, a liquid outlet channel, a sample inlet channel and a sample outlet channel are arranged on the quantitative channel, the quantitative channel is communicated with the liquid inlet channel and the liquid outlet channel, or the quantitative channel is communicated with the sample inlet channel and the sample outlet channel, and the quantitative channel is communicated with the liquid inlet channel and the liquid outlet channel or the sample inlet channel and the sample outlet channel selectively, so that the conversion of sample storage and quantitative sampling functions is realized.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a double T-shaped quantitative sampling microfluidic chip and a clamp applied to the chip.
Background
The micro-fluidic chip is a hotspot field of the development of the current micro total analysis system, and aims to integrate the functions of the whole laboratory, including sampling, dilution, reagent addition, reaction, separation, detection and the like, on the micro-chip and can be used for multiple times.
When the micro-fluidic chip samples, quantitative sampling needs to be carried out by matching with a six-way valve and a quantitative ring, and a six-way valve sample injector is an ideal sample injector in a high-efficiency liquid chromatography system and consists of a circular sealing pad and a fixed base. The working principle of the six-way valve sample injector is as follows: when the handle is positioned at the sampling position, the sample is injected into the quantitative ring from the sample inlet through the micro sample injection needle, and after the quantitative ring is filled, the redundant sample is discharged from the emptying hole; when the handle is rotated to the sample introduction position, the valve is communicated with the liquid phase flow path, the mobile phase conveyed by the pump washes the quantitative ring, and the sample is pushed to enter the liquid phase analysis column for analysis.
Although the sample liquid measurement of high accuracy can be realized to current appearance scheme of advancing, it is also comparatively convenient to use, but still has the problem: the six-way valve sample injector has a complex structure and large volume, is not easy to integrate in a microfluidic system, and is not beneficial to the miniaturization, portability and automation of the microfluidic system.
In addition, an important part of the application process of the microfluidic chip is the interface technology of the macroscopic fluid and the microscopic fluid, namely how to inject the macroscopic fluid into the microscopic chip pipeline. The traditional method is to connect an external sample injection pipeline through a steel needle, but the method has poor pressure resistance, the chip inlet is easy to damage when the steel needle is inserted into the chip, and the operation process is complex and is easy to introduce impurities.
Content of application
The invention aims to provide a double T-shaped quantitative sampling microfluidic chip and a clamp, which are used for solving the problems that the existing quantitative sampling equipment in the background art is complex in structure, high in cost, inconvenient to miniaturize and integrate and the inlet of the chip is damaged when a steel needle is inserted during sample injection.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a two "T" type ration sample micro-fluidic chip, includes the ration passageway, still includes the inlet channel with ration passageway intercommunication, goes out the liquid channel, and introduction channel and appearance passageway realize through the pressure differential of regulation and control liquid channel or appearance passageway: the inlet end of the quantitative channel is communicated with the liquid inlet channel, the outlet end of the quantitative channel is communicated with the liquid outlet channel, at the moment, the micro-fluidic chip is in a liquid inlet state, and the sample inlet channel and the sample outlet channel are closed; or the inlet end of the quantitative channel is communicated with the sample feeding channel, the outlet end of the quantitative channel is communicated with the sample discharging channel, the micro-fluidic chip is in a sampling state at the moment, the quantitative channel is used as the quantitative sample discharging channel, and the liquid inlet channel and the liquid outlet channel are closed.
Furthermore, the sample inlet channel and the sample outlet channel are arranged at two opposite ends of the quantitative channel, the liquid inlet channel and the liquid outlet channel are arranged on the side wall of the quantitative channel, and the liquid inlet channel and the liquid outlet channel are both communicated with the quantitative channel;
the inlet end of the liquid inlet channel is a liquid inlet, the outlet end of the liquid outlet channel is a liquid outlet, the inlet end of the sample introduction channel is a sample inlet, and the outlet end of the sample outlet channel is a sample outlet.
Further, feed liquor passageway and liquid outlet channel are located the both sides of ration passageway respectively, feed liquor passageway and ration passageway are "T" type, liquid outlet channel and ration passageway are "T" type of falling.
Further, the liquid inlet channel and the liquid outlet channel are both perpendicular to the quantitative channel.
Further, two "T" type ration sample micro-fluidic chip include apron layer, slot layer and the stratum basale that top-down set gradually, ration passageway, inlet channel, liquid outlet channel and introduction channel are the cavity that sets up between liquid circulation groove and the apron layer on the slot layer, apron layer bonding is on the slot layer, inlet, liquid outlet, introduction port and appearance mouth are all seted up on the stratum basale, the inlet passes through inlet channel intercommunication with ration channel, the liquid outlet passes through liquid outlet channel intercommunication with ration channel, introduction port and ration channel pass through introduction channel intercommunication, appearance mouth and ration channel pass through appearance channel intercommunication.
In addition, the invention also provides a clamp applied to the double T-shaped quantitative sampling microfluidic chip, which comprises a clamp box body, a clamp base and a clamp upper cover, wherein an accommodating cavity is formed in the clamp box body, the clamp base is placed in the accommodating cavity of the clamp box body, the microfluidic chip is placed on the clamp base, the clamp upper cover covers the clamp box body, and the clamp upper cover is abutted to the surface of the microfluidic chip.
Furthermore, a liquid flow channel communicated with the microfluidic chip is formed in the clamp base, four interfaces communicated with the liquid flow channel are formed in the upper surface of the clamp base, the liquid flow channel is respectively communicated with the liquid inlet, the liquid outlet, the sample inlet and the sample outlet of the microfluidic chip through the four interfaces, the positions and the sizes of the interfaces of the clamp base correspond to the positions of the liquid inlet, the liquid outlet, the sample inlet and the sample outlet of the microfluidic chip and are consistent, and sealing gaskets are arranged at the interfaces; the other end of the liquid flow channel is communicated with an external liquid storage device or a driving pump.
Further, the anchor clamps upper cover is opened and is established the observation window, the observation window sets up directly over the anchor clamps base, the edge of observation window sets up buffer washer.
Furthermore, the clamp base is detachably connected with the clamp box body, and the bottom of the clamp box body is provided with a power-assisted hole for taking the clamp base out; the side wall of the clamp box body is provided with a communication hole for communicating a liquid flow channel in the clamp base with the outside; the edge of the clamp box body is provided with an arc-shaped groove used for taking the microfluidic chip.
Further, the anchor clamps upper cover is articulated through articulated seat with one side of the anchor clamps box body, the anchor clamps upper cover sets up press switch with the anchor clamps box body for the opposite side of articulated seat, press switch is including pressing hasp and spring bolt, press the hasp setting on the anchor clamps base, press the spring bolt setting and cover on the anchor clamps, press the hasp and press the spring bolt and set up relatively.
Compared with the prior art, the invention has the beneficial effects that:
(1) The quantitative sampling micro-fluidic chip is simple in structure and convenient to use, only one circulation switching channel is arranged on the quantitative sampling micro-fluidic chip, namely the quantitative channel can be used as a sample storage channel and a quantitative sample outlet channel, when the quantitative sampling micro-fluidic chip is used, the pressure difference between a liquid outlet channel and the sample outlet channel on the micro-fluidic chip can be regulated and controlled by matching with an external driving pump and changing the pressure of the external driving pump, so that the quantitative channel is selectively communicated with a liquid inlet channel and the liquid outlet channel or selectively communicated with the sample inlet channel and the sample outlet channel, the conversion of the sample storage and quantitative sample outlet functions of the quantitative channel is realized, the micro-fluidic chip is further converted between a liquid inlet state and a sampling state, the quantitative sample liquid to be measured is accurately measured, and the layout difficulty and the process processing difficulty of the micro-fluidic chip are reduced.
(2) The invention provides a clamp applied to a double-T-shaped quantitative sampling micro-fluidic chip, which is adaptive to the size of the micro-fluidic chip, can be tightly clamped and sealed with the micro-fluidic chip, avoids the problems of damaging the chip inlet and introducing impurities when a steel needle is directly inserted into the micro-fluidic chip, and is additionally provided with an arc-shaped groove for taking the micro-fluidic chip, so that the micro-fluidic chip is convenient to take; an upper clamp cover which is tightly abutted against the microfluidic chip is arranged, and the upper clamp cover is detachably connected with the clamp box body, so that the upper clamp cover is convenient to open and close; the observation window is arranged to facilitate observation of whether state switching is carried out or not; the lateral part of the clamp box body is provided with a communicating hole for communicating the liquid flow channel with external liquid storage equipment or a driving pump, so that the micro-fluidic chip is conveniently communicated with the external liquid storage equipment and sampling equipment.
Drawings
FIG. 1 is an exploded view of a double "T" type quantitative sampling microfluidic chip according to the present invention;
FIG. 2 is a schematic top view of the double "T" type quantitative sampling microfluidic chip of the present invention;
FIG. 3 is a perspective view of the clamp of the present invention;
FIG. 4 is a perspective view of the base of the clamp of the present invention;
FIG. 5 is a schematic top view of the fixture base of the present invention;
FIG. 6 is a reference diagram illustrating the use state of the present invention;
in the figure: 1. a double T-shaped quantitative sampling micro-fluidic chip; 11. a cover plate layer 12, a groove layer; 121. a dosing channel; 122. a liquid inlet channel; 123. a liquid outlet channel; 124. a sample introduction channel; 125. a sample outlet channel; 13. a base layer; 131. a liquid inlet; 132. a liquid outlet; 133. a sample inlet; 134. a sample outlet; 2. a clamp; 21. a clamp box body; 211. pressing the lock catch; 212. a power-assisted hole; 213. a communicating hole; 214. an arc-shaped slot; 22. a clamp base; 23. an upper cover of the clamp; 231. an observation window; 232. pressing the lock tongue; 3. driving the pump; 4. a conduit; 5. and (4) a reaction tank.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Referring to fig. 1-6, the present invention provides a double "T" -type quantitative sampling microfluidic chip, as shown in fig. 1, the double "T" -type quantitative sampling microfluidic chip 1 includes a cover plate layer 11, a trench layer 12 and a substrate layer 13 sequentially disposed from top to bottom, the cover plate layer 11, the trench layer 12 and the substrate layer 13 are combined by a thermocompression bonding method, a liquid circulation groove is disposed on the trench layer 12, the cover plate layer 11 is bonded on the trench layer 12, a cavity between the cover plate layer 11 and the trench layer 12 is respectively provided as a quantitative channel 121, a liquid inlet channel 122, a liquid outlet channel 123, a sample inlet channel 124 and a sample outlet channel 125, the connection between the quantitative channel 121 and the liquid inlet channel 122 and the liquid outlet channel 123 is realized by adjusting and controlling the pressure difference between the liquid outlet channel 123 or between the quantitative channel 121 and the sample inlet channel 124 and the sample outlet channel 125, the connection between the quantitative channel 121 and the liquid inlet channel 122 and the liquid outlet channel 123 is realized by selecting, the connection between the quantitative channel 121 and the liquid inlet channel 124 and the sample outlet channel 125 is realized, and the conversion between the sample inlet state and the sample outlet state of the microfluidic chip is realized.
As shown in fig. 2, two ends of the quantitative channel 121 are an inlet end and an outlet end, respectively, the sample inlet channel 124 and the sample outlet channel 125 are disposed at two opposite ends of the quantitative channel 121, the liquid inlet channel 122 and the liquid outlet channel 123 are disposed at two sides of the quantitative channel 121, both the liquid inlet channel 122 and the liquid outlet channel 123 are communicated with the quantitative channel 121, both the liquid inlet channel 122 and the liquid outlet channel 123 are disposed perpendicular to the quantitative channel 121, both the liquid inlet channel 122 and the liquid outlet channel 121 are T-shaped, and both the liquid outlet channel 123 and the quantitative channel 121 are inverted T-shaped, as known in the art, the liquid inlet channel 122 and the liquid outlet channel 123 may also be disposed at the same side of the quantitative channel 121, and the liquid inlet channel 122 and the liquid outlet channel 123 may also be disposed at a certain angle from the side wall of the quantitative channel 121, in this embodiment, the liquid inlet channel 122 and the liquid outlet channel 123 are preferably disposed at two sides of the quantitative channel 121, and both the liquid inlet channel 122 and the liquid outlet channel 123 are disposed perpendicular to the quantitative channel 121, and the liquid outlet channel is preferably disposed at two sides of the quantitative channel 121, and the liquid inlet channel is disposed at two sides for preventing the liquid outlet channel 121, and the liquid outlet channel is disposed at the two sides for preventing the pump, and the liquid outlet channel 121; the inlet end of the liquid inlet channel 122 is a liquid inlet 131, the outlet end of the liquid outlet channel 123 is a liquid outlet 132, the inlet end of the sample inlet channel 124 is a sample inlet 133, the outlet end of the sample outlet channel 125 is a sample outlet 134, the liquid inlet 131, the liquid outlet 132, the sample inlet 133 and the sample outlet 134 are all arranged on the substrate layer 13, the liquid inlet 131 is communicated with the quantitative channel 121 through the liquid inlet channel 122, the liquid outlet 132 is communicated with the quantitative channel 121 through the liquid outlet channel 123, the sample inlet 133 is communicated with the quantitative channel 121 through the sample inlet channel 124, and the sample outlet 134 is communicated with the quantitative channel 121 through the sample outlet channel 125.
The working principle of the embodiment is as follows: when the inlet end of the quantitative channel 121 is communicated with the liquid inlet channel 122 and the outlet end of the quantitative channel 121 is communicated with the liquid outlet channel 123, the sample inlet channel 124 and the sample outlet channel 125 are closed and are not communicated, the microfluidic chip is in a liquid inlet state at this time, and the quantitative channel 121 is used as a sample inlet storage channel; the inlet end of the quantitative channel 121 is communicated with the sample inlet channel 124, the outlet end of the quantitative channel 121 is communicated with the sample outlet channel 125, the liquid inlet channel 122 and the liquid outlet channel 123 are closed and are not communicated, the microfluidic chip is in a sampling state, and the quantitative channel 121 is used as a quantitative sample outlet channel.
When the device is used, referring to fig. 6, the liquid inlet 131 of the liquid inlet channel 122 is communicated with an external liquid storage device, the sample outlet 134 of the sample outlet channel 125 is communicated with an external sampling device, the liquid inlet 131 is provided with positive pressure by a driving pump, the liquid outlet 132 of the liquid outlet channel 123 is provided with negative pressure by a driving pump, the sample inlet 133 of the sample inlet channel 124 and the sample outlet 134 of the sample outlet channel 125 are provided with positive pressure by a driving pump, and the liquid outlet channel 123 is used for providing positive pressure with pressure difference with other liquid inlet channels 122, sample inlet channels 124 and sample outlet channels 125, so that sample liquid to be detected enters the microfluidic chip through the liquid inlet 131 of the liquid inlet channel 122, the quantitative channel 121 is used as a sample inlet storage channel, when liquid enters the liquid outlet channel 123, it is indicated that the quantitative channel 121 is filled with sample liquid to be detected, and the microfluidic chip is in a sample inlet storage state; then, a positive pressure is provided at the liquid inlet 131 by a driving pump, a positive pressure is provided at the liquid outlet 132 of the liquid outlet channel 123 by a driving pump, a positive pressure is provided at the sample inlet 133 of the sample inlet channel 124 by a driving pump, a negative pressure is provided at the sample outlet 134 of the sample outlet channel 125 by a driving pump, and a pressure difference between the sample outlet channel 125 and the other liquid inlet channels 122, the liquid outlet channel 123 and the sample inlet channel 124 is utilized, so that the sample liquid to be measured flows out of the microfluidic chip from the quantitative channel 121 through the sample outlet 134 of the sample outlet channel 125, and the quantitatively measured sample liquid enters the reaction tank 5 for reaction (or enters other sampling devices), and at this time, the quantitative channel 121 is used as a quantitative sample outlet channel.
The micro-fluidic chip has a simple structure, is matched with an external driving pump 3, is provided with only one quantitative channel as a circulation switching channel, namely the quantitative channel can be used as a sample storage channel and also can be used as a quantitative sample outlet channel, and when the micro-fluidic chip is used, the pressure difference between the liquid outlet channel and other channels on the micro-fluidic chip (or the pressure difference between the sample outlet channel and other channels) can be regulated and controlled by changing the pressure provided by the driving pump 3 to the liquid inlet 131, the liquid outlet 132, the sample inlet 133 and the sample outlet 134, namely the conversion of the sample storage and quantitative sample outlet functions of the quantitative channel can be realized, so that the micro-fluidic chip is converted between the sample storage state and the quantitative sample outlet state, the quantitative sample liquid to be measured can be accurately measured, the layout difficulty and the process processing difficulty of the micro-fluidic chip are reduced, and the micro-scale and integration are convenient.
The cover plate layer 11, the groove layer 12 and the substrate layer 13 are all made of organic polymer materials, preferably polymethyl methacrylate (PMMA) materials, which have the characteristics of toughness, hardness, moderate price, easy machining, especially excellent biocompatibility, no pollution to biological cells, and transparent materials which are easy to observe; but the scratch resistance is poor, and the interface of the needle is easy to be damaged by the traditional steel needle when in use.
Aiming at the double T-shaped quantitative sampling microfluidic chip 1, in order to avoid damaging a microfluidic chip interface in use, the invention also provides a clamp 2 applied to the double T-shaped quantitative sampling microfluidic chip, please refer to fig. 3-5, wherein the clamp 2 comprises a clamp box body 21, a clamp base 22 and a clamp upper cover 23, an accommodating cavity for accommodating the chip is formed in the clamp box body 21, the size of the accommodating cavity is consistent with that of the microfluidic chip, the clamp base 22 is arranged in the accommodating cavity of the clamp box body 21, the microfluidic chip is arranged on the clamp base 22, the clamp box body 21 can be tightly clamped and sealed with the microfluidic chip, and the microfluidic chip cannot shake in the clamp box body 21; the edge of the clamp box body 21 is provided with two arc-shaped grooves 214 for facilitating taking of the microfluidic chip, and in a further optimization, the two arc-shaped grooves 214 are oppositely arranged at the edge of the clamp box body 21, so that clamping, loading and position adjustment can be conveniently carried out from the side part of the microfluidic chip.
The clamp upper cover 23 covers the clamp box body 21, the clamp upper cover 23 abuts against the surface of the microfluidic chip, the clamp upper cover 23 is provided with an observation window 231, the observation window 231 is arranged above the clamp base 22 and used for observing the liquid inlet or outlet state on the microfluidic chip and judging whether to switch the liquid inlet state or the sampling state, and a buffer gasket is arranged on the outer edge of the observation window 231 to increase the buffer and prevent the microfluidic chip from being damaged in the covering and abutting process of the clamp upper cover 23;
the clamp upper cover 23 is hinged to one side of the clamp box body 21 through the hinged seat, the clamp upper cover 23 and the clamp box body 21 are provided with a press switch relative to the other side of the hinged seat, the press switch comprises a press buckle 211 and a press bolt 232, the press buckle 211 is arranged on the clamp base 22, the press bolt 232 is arranged on the clamp upper cover 23, the press buckle 211 and the press bolt 232 are arranged oppositely, and the press buckle 211 and the press bolt 232 facilitate opening and closing of the clamp upper cover 23.
The clamp base 22 and the clamp box body 21 are detachable, the outer contour dimension of the clamp base 22 is equal to the length and width of the accommodating cavity of the clamp box body 21, the clamp base 22 is just embedded in the accommodating cavity of the clamp box body 21, the height of the clamp base 22 is slightly lower than that of the accommodating cavity of the clamp box body 21, when the clamp base 22 is placed in the accommodating cavity of the clamp box body 21, the height difference between the upper surface of the clamp base 22 and the height difference between the upper surface of the clamp box body 21 are just the thickness of a chip, a liquid flow channel communicated with the microfluidic chip is arranged in the clamp base 22, one end of the liquid flow channel extends to the upper surface of the clamp base 22, four interfaces communicated with the liquid flow channel are arranged on the upper surface of the clamp base 22, the liquid flow channel is respectively communicated with a liquid inlet 131, a liquid outlet 132, a sample inlet 133 and a sample outlet 134 of the microfluidic chip through the four interfaces, the positions and the sizes of the interfaces are corresponding to the positions of the liquid inlet 131, the liquid outlet 132, the sample inlet 133 and the sample outlet 134 of the chip, and the interfaces are consistent, and the sizes of the interfaces are all provided with sealing gaskets, the sealing gaskets can ensure the sealing performance of the sealing during the liquid leakage of the microfluidic chip, and can be increased simultaneously, the buffering base can prevent the damage to the clamp base caused by the damage to the microfluidic chip in the capping process; the other end of the liquid flow channel is communicated with an external liquid storage device or a driving pump 3; the bottom of the clamp box body 21 is provided with a power assisting hole 212 for assisting in taking out the clamp base 22, and when the clamp is used, an upward force is applied to the clamp base at the power assisting hole 212, so that the clamp base can be conveniently taken out of the clamp box body; the side of the clamp box body 21 is provided with a communication hole 213 for communicating the liquid flow channel with an external liquid storage device or a drive pump 3, so that the microfluidic chip can be conveniently communicated with the external device.
The driving pumps 3 are all optimized to be Lange micro plunger pumps, the models are MP500-2L-A1C2000 or MP250-2L-A1C2000, and the driving pumps are connected with the clamp base 22 through the guide pipes 4 and the steel needles, so that the problems that the chip inlets are damaged and impurities are introduced when the steel needles are directly inserted into the microfluidic chip are avoided; the guide pipe is preferably a PTFE (polytetrafluoroethylene) pipe, the Lange miniature plunger pump is suitable for high-precision high-stability microfluid sample injection, the zero calibration is carried out by adopting a precise transmission design and ultra-high-precision parts, and a high-reliability photoelectric sensor is adopted, so that the full stroke accuracy is high, the high-precision trace fluid transmission is realized, the analysis result can be ensured, the consumption of samples and reagents can be reduced, and the cost is saved; in order to facilitate the control of the driving pumps, a plunger pump Controller is configured to regulate and control the pressure of each channel, and the Controller preferably selects a USBCNC Controller control panel (an industrial standard four-axis CNC Controller) to simultaneously control the four driving pumps to work in a linkage manner.
Claims (10)
1. The utility model provides a two "T" type ration sample micro-fluidic chip which characterized in that: including ration passageway (121), still include inlet channel (122) with ration passageway (121) intercommunication, go out liquid channel (123), advance kind passageway (124) and go out appearance passageway (125), realize through the pressure differential of regulation and control liquid channel (123) or play appearance passageway (125): the inlet end of the quantitative channel (121) is communicated with the liquid inlet channel (122), the outlet end of the quantitative channel (121) is communicated with the liquid outlet channel (123), at the moment, the microfluidic chip is in a liquid inlet state, the quantitative channel (121) is used as a sample storage channel, and the sample inlet channel (124) and the sample outlet channel (125) are closed; or the inlet end of the quantitative channel (121) is communicated with the sample feeding channel (124), the outlet end of the quantitative channel (121) is communicated with the sample discharging channel (125), the microfluidic chip is in a sampling state at the moment, the quantitative channel (121) is used as the quantitative sample discharging channel, and the liquid inlet channel (122) and the liquid outlet channel (123) are closed.
2. The double "T" shaped quantitative sampling microfluidic chip of claim 1, wherein: the sample inlet channel (124) and the sample outlet channel (125) are arranged at two opposite ends of the quantitative channel (121), the liquid inlet channel (122) and the liquid outlet channel (123) are arranged on the side wall of the quantitative channel (121), and the liquid inlet channel (122) and the liquid outlet channel (123) are both communicated with the quantitative channel (121);
the entrance end of inlet channel (122) is inlet (131), the exit end of outlet channel (123) is liquid outlet (132), the entrance end of introduction channel (124) is introduction port (133), the exit end of appearance passageway (125) is appearance mouth (134).
3. The double "T" shaped quantitative sampling microfluidic chip of claim 2, wherein: the liquid inlet channel (122) and the liquid outlet channel (123) are respectively positioned at two sides of the quantitative channel (121), the liquid inlet channel (122) and the quantitative channel (121) are T-shaped, and the liquid outlet channel (123) and the quantitative channel (121) are inverted T-shaped.
4. The double "T" shaped quantitative sampling microfluidic chip of claim 3, wherein: the liquid inlet channel (122) and the liquid outlet channel (123) are both vertical to the quantitative channel (121).
5. The double "T" type quantitative sampling microfluidic chip of claim 4, wherein: two "T" type ration sample micro-fluidic chip includes apron layer (11), groove layer (12) and stratum basale (13) that top-down set gradually, ration passageway (121), inlet channel (122), liquid outlet channel (123) and introduction of sample passageway (124) are the cavity that sets up between liquid circulation groove and apron layer (11) on groove layer (12), apron layer (11) bonding is on groove layer (12), inlet (131), liquid outlet (132), introduction port (133) and appearance mouth (134) are all seted up on stratum basale (13), inlet (131) and ration passageway (121) pass through inlet channel (122) intercommunication, liquid outlet (132) and ration passageway (121) pass through liquid outlet channel (123) intercommunication, introduction port (133) and ration passageway (121) pass through introduction of sample passageway (124) intercommunication, appearance mouth (134) and ration passageway (121) pass through appearance (125) intercommunication.
6. A clamp applied to the double T-shaped quantitative sampling microfluidic chip of claims 2 to 5, is characterized in that: including anchor clamps box body (21), anchor clamps base (22) and anchor clamps upper cover (23), the holding chamber is seted up to anchor clamps box body (21) inside, the holding intracavity at anchor clamps box body (21) is placed in anchor clamps base (22), place micro-fluidic chip on anchor clamps base (22), anchor clamps upper cover (23) lid closes on anchor clamps box body (21), anchor clamps upper cover (23) butt is on micro-fluidic chip's surface.
7. The clamp applied to the double T-shaped quantitative sampling microfluidic chip of claim 6, wherein: a liquid flow channel communicated with the microfluidic chip is formed in the clamp base (22), four interfaces communicated with the liquid flow channel are arranged on the upper surface of the clamp base (22), the liquid flow channel is respectively communicated with a liquid inlet (131), a liquid outlet (132), a sample inlet (133) and a sample outlet (134) of the microfluidic chip through the four interfaces, the positions and the sizes of the interfaces of the clamp base (22) correspond to the positions of the liquid inlet (131), the liquid outlet (132), the sample inlet (133) and the sample outlet (134) of the microfluidic chip and are consistent, and sealing gaskets are arranged at the interfaces; the other end of the liquid flow channel is communicated with an external liquid storage device or a driving pump (3).
8. The clamp applied to the double T-shaped quantitative sampling microfluidic chip according to claim 7, wherein: an observation window (231) is formed in the clamp upper cover (23), the observation window (231) is arranged right above the clamp base (22), and a buffer gasket is arranged on the edge of the observation window (231).
9. The clamp applied to the double T-shaped quantitative sampling microfluidic chip according to claim 8, wherein: the clamp base (22) is detachably connected with the clamp box body (21), and the bottom of the clamp box body (21) is provided with a power-assisted hole (212) for taking out the clamp base (22); the side wall of the clamp box body (21) is provided with a communication hole (213) for communicating a liquid flow channel in the clamp base (22) with the outside; the top edge of the clamp box body (21) is provided with an arc-shaped groove (214) for taking the microfluidic chip.
10. The clamp applied to the double T-shaped quantitative sampling microfluidic chip of claim 9, wherein: the anchor clamps upper cover (23) are articulated through articulated seat with one side of anchor clamps box body (21), anchor clamps upper cover (23) and anchor clamps box body (21) set up press the switch for the opposite side of articulated seat, press the switch including pressing hasp (211) and pressing spring bolt (232), press hasp (211) to set up on anchor clamps base (22), press spring bolt (232) to set up on anchor clamps upper cover (23), press hasp (211) and press spring bolt (232) and set up relatively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211242696.XA CN115837290A (en) | 2022-10-11 | 2022-10-11 | Double T-shaped quantitative sampling micro-fluidic chip and clamp applied to chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211242696.XA CN115837290A (en) | 2022-10-11 | 2022-10-11 | Double T-shaped quantitative sampling micro-fluidic chip and clamp applied to chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115837290A true CN115837290A (en) | 2023-03-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211242696.XA Pending CN115837290A (en) | 2022-10-11 | 2022-10-11 | Double T-shaped quantitative sampling micro-fluidic chip and clamp applied to chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115837290A (en) |
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2022
- 2022-10-11 CN CN202211242696.XA patent/CN115837290A/en active Pending
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