CN115812707A - Locust agglutinin attractant composition - Google Patents
Locust agglutinin attractant composition Download PDFInfo
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- CN115812707A CN115812707A CN202211394910.3A CN202211394910A CN115812707A CN 115812707 A CN115812707 A CN 115812707A CN 202211394910 A CN202211394910 A CN 202211394910A CN 115812707 A CN115812707 A CN 115812707A
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- attractant
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- 239000000203 mixture Substances 0.000 title claims abstract description 158
- 239000005667 attractant Substances 0.000 title claims abstract description 83
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 83
- 101710186708 Agglutinin Proteins 0.000 title claims abstract description 65
- 101710146024 Horcolin Proteins 0.000 title claims abstract description 65
- 101710189395 Lectin Proteins 0.000 title claims abstract description 65
- 101710179758 Mannose-specific lectin Proteins 0.000 title claims abstract description 65
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 title claims abstract description 65
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 title claims abstract description 65
- 239000000910 agglutinin Substances 0.000 title claims abstract description 65
- 239000002904 solvent Substances 0.000 claims abstract description 65
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- 238000000034 method Methods 0.000 claims abstract description 7
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- ZPVFWPFBNIEHGJ-UHFFFAOYSA-N 2-octanone Chemical compound CCCCCCC(C)=O ZPVFWPFBNIEHGJ-UHFFFAOYSA-N 0.000 claims description 16
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- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 claims description 7
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 6
- GDESWOTWNNGOMW-UHFFFAOYSA-N resorcinol monobenzoate Chemical compound OC1=CC=CC(OC(=O)C=2C=CC=CC=2)=C1 GDESWOTWNNGOMW-UHFFFAOYSA-N 0.000 claims description 6
- ZQBAKBUEJOMQEX-UHFFFAOYSA-N salicylic acid phenyl ester Natural products OC1=CC=CC=C1C(=O)OC1=CC=CC=C1 ZQBAKBUEJOMQEX-UHFFFAOYSA-N 0.000 claims description 6
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- 229940057995 liquid paraffin Drugs 0.000 claims description 5
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 claims description 5
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- ZXDDPOHVAMWLBH-UHFFFAOYSA-N 2,4-Dihydroxybenzophenone Chemical compound OC1=CC(O)=CC=C1C(=O)C1=CC=CC=C1 ZXDDPOHVAMWLBH-UHFFFAOYSA-N 0.000 claims description 4
- 235000010354 butylated hydroxytoluene Nutrition 0.000 claims description 4
- 238000013268 sustained release Methods 0.000 claims description 4
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- YZAZXIUFBCPZGB-QZOPMXJLSA-N (z)-octadec-9-enoic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O.CCCCCCCC\C=C/CCCCCCCC(O)=O YZAZXIUFBCPZGB-QZOPMXJLSA-N 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
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- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 3
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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- RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention particularly relates to a locust agglutinin attractant composition, which belongs to the field of plant protection and comprises the following components: locust agglutinin, slow release solvent, synergist and protectant; the method is characterized in that: the locust agglutinin attractant composition comprises the following components in percentage by mass: 5-12% of locust agglutinin, 85-92% of slow release solvent, 0.9-3.6% of synergist and 0.8-2.2% of protective agent. The locust aggregation element attractant composition has the characteristics of strong specificity on the target pests of locust, good trapping effect, stable trapping and long lasting period, and can meet the actual requirements of high-efficiency monitoring and accurate control on the field target locust.
Description
Technical Field
The invention belongs to the field of plant protection, and particularly relates to a locust agglutinin attractant composition.
Background
Locusts belong to the general locusts of the order orthoptera, and are of various species and wide in distribution. The species of locusts all over 1 million in the world, more than 500 kinds of locusts are mainly harmful to grassland and crops, wherein the main locusts harmful to the gramineae family are migratory locusts, the main locusts harmful to pasture is dolly locusts, and the locusts eating other crops are like sword locusts, water chestnut locusts, cotton locusts, locusts and locusts.
Currently, in the research on the locusts, the locusts have obvious tropism and aggregation on some substances, such as the excrement of the locusts and other animal excretions. However, the finished locust agglutinin attractant on the market has fewer varieties and unobvious trapping effect; there are mainly the following disadvantages, such as: the bait has the advantages of single pest species, unstable effect, poor trapping effect, large quantity of non-target pests, more pests, short product duration, small trapping amount of locust nymphs, unstable effect and short duration due to the influence of environmental factors such as temperature and sunlight on the bait when the bait is applied in the field.
In order to solve a series of problems of the existing locust luring, the invention aims to develop a high-efficiency and specific locust agglutinin attractant composition.
Disclosure of Invention
According to the actual requirements of locust control and monitoring, the invention develops the insect pheromone attractant composition for spodoptera frugiperda, which better overcomes the problems and the defects existing in the prior art.
The technical scheme is as follows:
a locust agglutinin attractant composition comprising: locust agglutinin, slow release solvent, synergist and protectant; the method is characterized in that: the locust agglutinin attractant composition comprises, by mass, 5-12% of locust agglutinin, 85-92% of slow release solvent, 0.9-3.6% of synergist and 0.8-2.2% of protective agent.
The locust aggregation element attractant composition comprises locust aggregation element, a slow release solvent, a synergist and a protective agent, the locust aggregation element, the slow release solvent, the synergist and the protective agent are screened and reasonably proportioned, so that the number of the locust attractant composition can be further increased, the effective period of the locust attractant composition can be further prolonged, the releasing rate of the locust pheromone composition is consistent, the odor of the locust pheromone composition is stable, and the types and the proportion of the non-target pests to the other pests can be reduced.
Further, the locust agglutinin comprises the following components: guaiacol, phenol, trans-2-pentanal, hexanal, cis-3-hexenol, benzyl alcohol, alpha-methyl-beta-ethylacrolein, 2-octanone, 2-heptanone and 2,5-dimethylpyrazine.
The components are agglutinin components analyzed and extracted from excrement and bodies of locust pests, and have aggregation specificity to target pests, wherein guaiacol and alpha-methyl-beta-ethylacrolein are main trapping components of the locust pests, have a leading effect on the number of the trapped locust, and can directly influence the trapping number and trapping effect of the locust if relevant components are removed; the benzyl alcohol has aromatic taste, has certain tendency to locust, can also be used as a fixative to increase the lasting period and ensure that the properties of the attractant composition are more stable; the phenol can promote the odor of the locust which is released by the attractant composition to be more preferred, the aggregation effect of the locust agglutinin on the locust is increased, and the alpha-methyl-beta-ethylacrolein can effectively inhibit the development of the locust nymph.
Further, the locust agglutinin comprises the following components in parts by mass: 0.2 to 2 portions of guaiacol, 1 to 10 portions of phenol, 0.5 to 2 portions of trans-2-pentanal, 1 to 4 portions of hexanal, 1 to 3 portions of cis-3-hexenol, 1 to 2 portions of benzyl alcohol, 0.5 to 10 portions of alpha-methyl-beta-ethylacrolein, 0.5 to 4 portions of 2-octanone, 0.5 to 4 portions of 2-heptanone and 1 to 5 portions of 2,5-dimethylpyrazine. The components and the mass content achieve the best effects on trapping effect, stability, lasting period and the like of the target locust, and meanwhile, the variety of the mixed insects of the non-target pests is reduced in the gathering process of the pests.
Further, the slow-release solvent comprises a quick slow-release solvent and a uniform slow-release solvent, wherein the quick dilution solvent is one or a combination of n-hexane and petroleum ether; the uniform speed sustained-release solvent is one or more of (Z) -9-octadecenoic acid (oleic acid), liquid paraffin oil and isopropyl myristate. The slow release solvent is mainly used for dissolving each component of the locust aggregation pheromone to help the locust aggregation pheromone to realize a slow release effect, has stable slow release rate, can be used as a solvent, and can be added in the production process. Wherein, the composition 1 is mainly used for rapidly fusing and uniformly mixing the effective component substances to ensure that the effective components of the locust agglutinin form a homogeneous mixture; the composition 2 mainly has the effect of solving a series of problems of waste, short duration and the like caused by the fact that the first composition is too fast in slow release and the release speed of the active ingredients is fast.
Further, the two compositions of the slow-release solvent are as follows by mass: 1-1.5 parts of fast diluting solvent and 6-9 parts of uniform slow-release solvent. The composition 1 and the composition 2 are mixed according to the proportion, so that the active ingredient of the locust agglutinin can be released at a constant speed, the trapping effect is more stable, and the lasting period is longer.
Furthermore, the synergist is one or a combination of more of leaf alcohol, benzaldehyde and wheat bran essence. The synergist is mainly used for further increasing the trapping effect of the locust aggregation pheromone composition, and the components and proportion of the synergist are selected according to the components and the total amount of the locust aggregation pheromone composition so as to achieve the optimal synergistic effect.
Further, the protective agent comprises two compositions, wherein,
the composition a is: one or more of phenyl ortho-hydroxybenzoate, 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole, 2,4-dihydroxybenzophenone, and resorcinol monobenzoate;
the composition b is one or more of triphosphite, vitamin E, 2,6-ditert-butyl-p-cresol.
The screening of the protective agent components is mainly considered, and the environmental factors of the locust agglutinin attractant in the field application process comprise: when the sunlight, the temperature and the humidity change, the release stability among different compositions of the locust agglutinin is influenced, so that the trapping quantity is reduced and the lasting period is shortened. Thus, the use of a protectant reduces the effect of external environmental factors on the stability and duration of the attractant component composition. The composition a comprises phenyl o-hydroxybenzoate, 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole, 2,4-dihydroxybenzophenone and resorcinol monobenzoate, and mainly acts as an ultraviolet absorber (anti-ultraviolet), so that the active ingredients are more stable when used in the field, can be combined with a carrier, and the service life of the carrier is longer; the composition b contains triphosphite, vitamin E and 2,6-di-tert-butyl-p-cresol, and mainly has the effect of preventing a series of problems of short lasting period, unstable effect and the like caused by oxidation of effective components and used carriers.
Further, two compositions of the protective agent, each composition by mass: 1 to 1.5 portions of the composition a and 1 to 3 portions of the composition b. The two compositions are mixed according to a proper mass ratio, so that the effective components and the carrier can be released more stably, the lasting period is prolonged, and the inhibiting effect of improper content of the protective agent components on the effective components of the agglutinin can be avoided.
Further, the composition can be used for gathering and luring locusts of various ages such as locusts migratorius, locusts, locusts in desert and locusts in phyllostachys calcarata, and during the luring process, the male, female, adult and larva of the locusts of the types can achieve the luring effect.
Further, the composition can be applied alone or in combination with a carrier, comprising: the locust attractant is made of rubber, PVC (polyvinyl chloride), fluorescent silica gel and polyisoprene or the locust attractant is matched with the composition for use. The composition of the attractant is matched with a carrier for use, so that the duration and the attraction effect of the attractant can be increased, the use convenience is also increased, the attractant is better suitable for multiple occasions and more complex environments, and particularly matched with locust monitoring equipment, the effect is better; the attractant composition and the carrier are matched for use in a way that: dripping, soaking, filling and the like.
In conclusion, the locust aggregation hormone attractant composition provided by the invention extracts and screens effective components capable of inducing aggregation of locust through locust feces, and realizes good attraction effect on both main kinds of locust and locust nymphs through proper proportion of the components; the sustained-release agent promotes the full dissolution of each component of the locust aggregation pheromone by the components and the proportion of the sustained-release solvent, realizes the continuous and stable release of the active components of the agglutinin, adds reasonable synergist and mass proportion according to the preference of the locust, and has the optimal synergistic effect with other achievements of the agglutinin attractant. Meanwhile, the protective agent is added into the composition, the proper protective agent and the mass content thereof are selected according to each component of the attractant composition, the effective period of the composition can be prolonged, the field applicability of the locust aggregation attractant composition is increased, the attractant composition can still realize continuous and stable release even under the conditions of high temperature, high humidity and high-intensity sunlight irradiation, the effective period of the locust aggregation attractant composition can reach 60 days, and the rate of the undesired insects is less than 1 percent.
The specific implementation mode is as follows:
the present invention is further illustrated by the following examples, which should not be construed as limiting the invention thereto.
Example 1:
the locust agglutinin attractant composition comprises the following components in percentage by mass: 7.2 percent of locust agglutinin, 87 percent of slow release solvent, 3.6 percent of synergist and 2.2 percent of protective agent; wherein the slow release solvent comprises the following components in percentage by mass: 1.5 parts of a quick slow-release solvent which is n-hexane and 9 parts of a uniform slow-release solvent, wherein 6 parts of (Z) -9-octadecenoic acid, 3 parts of isopropyl myristate and a synergist are benzaldehyde; the protective agent comprises the following components in percentage by mass: composition a is 1.5 parts, wherein the amount of anthranilate is 0.2 parts, the amount of 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole is 0.5 parts, and the amount of resorcinol monobenzoate is 0.8 parts; the composition b is 1 part, wherein the vitamin E is 0.5 part, and the 2,6-ditertbutyl-p-cresol is 0.5 part. The locust agglutinin comprises the following components in parts by mass, wherein the addition amount is based on standard mass number and is added according to the parts required by related components.
Table 1: the locust agglutinin comprises the following components in percentage by mass: portions are
1-8 of locust agglutinin attractant composition composed of the above components by mass.
Meanwhile, the prior art is taken as a control to carry out locust luring observation;
under the condition that the components and contents of the slow-release solvent, the synergist and the protective agent are the same, the compound without the locust agglutinin is blank CK.
Test site: hebei Cangzhou yellow Ye south Dagang wetland
Time: 7-9 months in 2021
The locust aggregation hormone attractant compositions 1 to 8 and the composition CK are respectively smeared on the same locust attracting device, each trapper is spaced by 100 meters, a random block design is adopted, each treatment is repeated for five times, the investigation is continued for 30 days, the number of attracted target pests and the number of the attracted pests are recorded in the morning of each day, and the statistics is carried out by taking the average of the composition CK and the five repetitions of the compositions 1 to 8: the total amount of the lures, the number of the locusts, the number of the mixed insects, the number of various locusts, the number of male insects, the number of female insects and the number of nymph cells of the locusts are counted, and the proportion of the mixed insects and the proportion of the nymph cells of the locusts are counted.
Table 2: locust trapping effect analysis of locust agglutinin attractant composition
Unit: head with a rotatable shaft
In the test, the target pest trapping test is carried out in the northern river Cangzhou for 30 days, and the results are as follows:
locust agglutinin attractant compositions 1-5 trap pests comprising: the total number of the lured locusts reaches more than 230, the number of the lured locusts reaches more than 220, the total number of the lured locusts and the lured locusts is the largest, the luring total number of the composition 3 is 296, and the number of the locusts can reach 280. From the view of the number of the trapped mixed insects, the number of the trapped mixed insects is not more than 20, the mixed insect rate is 3.90-6.00%, and the mixed insect rate is low. From the number of trapped locust nymphs, the locust agglutinin attractant composition 1-5 is more than 70, the proportion of the locust nymphs is more than 30%, at most 35.54%, and the trapping of males and females can be realized simultaneously.
Compared with the locust aggregation element attractant composition 1-5, the locust aggregation element attractant composition 6-8 which lacks one or more locust aggregation element components has the advantages of small total amount of attracted insects, high rate of undesired insects and low proportion of locust nymphs, the minimum total amount of attracted insects is that the composition 7 is only 110 heads, the maximum is that the composition 6 is 157 heads, the rate of undesired insects is 10.83-12.41%, which is 2-3 times of the locust aggregation element attractant composition 1-5, the rate of undesired insects is higher, the proportion of attracted insects is only 10.83-12.37%, and is only 1/3 of the proportion of locust nymphs of the locust aggregation element attractant composition 1-5.
The difference between the total amount of the locust lures and 6-8 of the locust agglutinin attractant composition in the prior art is not large, but the rate of the undesired insects is high, the proportion of locust nymphs is low, only 5.77%, and the proportion of the locust nymphs can directly influence the prevention and control effect of target pests. On the current locust trapping, for example, the locust is trapped by a locust trapping product, the locust nymph proportion is high, the product is more beneficial to the prevention and control of target pests, is more suitable for the prevention and control test of the locust, and has good field applicability.
However, the blank CK without any component of the locust agglutinin basically has no aggregation effect on the locust, and the total amount of the locust lured, the rate of the undesired insects and the proportion of locust nymphs are the worst.
In conclusion: the locust aggregation element composition and the content have good effects on the total locust attractant amount of the locust aggregation element attractant composition, the reduction of the rate of the mixed insects, the improvement of the locust nymph proportion and the main locust species of the trapping set, and the balance of the female and male locust number of the locust. Meanwhile, in subsequent tests, the locust aggregation element attractant composition 1-5 can still continuously trap the target locust, and the comprehensive lasting period can reach more than 60 days.
Example 2:
the locust agglutinin attractant composition comprises the following components in percentage by mass: 8.8% of locust agglutinin, 89.5% of slow release solvent, 0.9% of synergist and 0.8% of protective agent, wherein the locust agglutinin comprises the following components: 0.5 part of guaiacol, 6 parts of phenol, 1.25 parts of trans-2-pentanal, 3.5 parts of hexanal, 2.1 parts of cis-3-hexenol, 1.7 parts of benzyl alcohol, 1 part of alpha-methyl-beta-ethylacrolein, 2 parts of 2-octanone, 2.3 parts of 2-heptanone and 1.5 parts of 2,5-dimethylpyrazine; the components and the mass ratio of the synergist are respectively as follows: a combination of benzaldehyde and wheat bran essence; the protective agent comprises the following components in percentage by mass: 1.25 parts of composition a, wherein the amount of the o-hydroxybenzoate is 0.25 part and the amount of the resorcinol monobenzoate is 1 part; and the composition b is 3 parts, wherein: 0.6 part of triphosphite, 1 part of vitamin E and 1.4 parts of 2,6-ditertbutyl-p-cresol. The components and mass contents of the slow release solvent are shown in the table below, and the addition amount of the slow release solvent is the addition amount of the slow release solvent by taking a standard mass number as a unit, and is added according to the required parts of the related components.
Table 3: each component and mass content of slow-release solvent
Unit: portions are
Locust agglutinin attractant composition 1-7 composed of the above components and by mass.
Meanwhile, under the condition that the components and the contents of the locust aggrecan, the synergist and the protective agent are the same, the slow-release solvent is not added, and the compound is used as a composition CK;
test site: mongolian yunnan region
Time: 7-9 months in 2021
The locust aggregation element attractant compositions 1 to 7 and the composition CK are independently smeared on the same locust attracting device, each trapper is separated by 20 meters, a random block design is adopted, each treatment is repeated for five times, continuous investigation is carried out, a lasting period (the lasting period refers to the accumulated days for attracting target pests) is recorded, the average number of the locust attracted by the compositions 1 to 7 and the composition CK in five repetitions and the number of the locust attracted by 10 days in the morning are recorded, and the related data are shown in the following table:
table 4: locust trapping effect analysis of locust agglutinin attractant composition
Unit: head with a rotatable shaft
In the test, the test period is 60 days, the observation and the recording are carried out for more than 60 days, and if the pests are not trapped for 5 consecutive days, the time from the arrangement date to 5 days is recorded as the effective period;
from the above table, it can be seen that: the compositions 1-5 have good performances in the aspects of lasting period, locust number, daily average locust trapping amount, the recorded locust trapping amount by taking 10 days as a unit and the like, can stably trap target locust in an observed test period of 60 days, and have small change of trapping effect along with the extension of test days. The total amount of the trapped locusts can reach more than 360 heads, wherein the composition 5 has the best performance, the total amount of trapped locusts in 60 days can reach 428 heads, the daily average trapping amount is 7.13 heads, and the trapping amount of target locusts in stages by taking 10 days as a unit is 65-81 heads/10 days.
Compared with the composition 1, the composition 6 which only lacks a quick slow release solvent and has the same other components and mass contents has the trapping lasting period of 60 days, but the locust number and the daily average trapping amount are obviously reduced, the total trapping amount is only 109, the daily average trapping amount is 1.82, and the staged trapping amount is only 32 at most in 10 days. Therefore, the quick slow-release solvent has an important effect on the sufficient and efficient release of the locust agglutinin component.
Compared with the composition 3, the composition 7 only lacks a uniform-speed slow-release solvent, has the same other components and mass contents, has obviously reduced induction lasting period of only 28 days, obviously reduced total induction quantity and daily average induction quantity, only 107 induction total quantity and 3.82 induction quantity, has little change of unit induction quantity with the composition 2 in 1-10 days in the initial test period and 69 induction quantity, and has sharply reduced induction quantity in the initial test period along with the prolonging of the test time. Therefore, the uniform-speed slow-release solvent has an important function on the continuous and stable release of the locust agglutinin component.
The CK composition lacking the slow-release solvent is poor in performance of the effective period, the total pest trapping amount and the daily average pest trapping amount, the total pest trapping amount is only 10, the effective period is 12 days, and the pest trapping amount is only 7 heads in 1-10 days in the initial period of the test.
In conclusion: the slow-release solvent has a vital function in the locust aggregation attractant composition, directly influences the locust induction amount, the lasting period and the stable insect induction effect, and meanwhile, the interaction of the rapid slow-release solvent and the uniform slow-release solvent in the slow-release solvent prolongs the lasting period of the slow-release solvent in the locust aggregation attractant composition to 60 days, and the slow-release solvent is released stably in the whole induction period, and the stage insect induction amount and the induction effect are not obviously changed.
Example 3:
the locust agglutinin attractant composition comprises the following components in percentage by mass: 5% of locust agglutinin, 92% of slow release solvent, 1.8% of synergist and 1.2% of protective agent; wherein, the locust agglutinin comprises the following components: 1.4 parts of guaiacol, 4 parts of phenol, 1.9 parts of trans-2-pentanal, 2.8 parts of hexanal, 2.8 parts of cis-3-hexenol, 1.1 parts of benzyl alcohol, 2.8 parts of alpha-methyl-beta-ethylacrolal, 2.8 parts of 2-octanone, 3.7 parts of 2-heptanone and 4.2 parts of 2,5-dimethylpyrazine; the slow-release solvent comprises the following components in parts by mass: 1 part of a quick slow-release solvent, wherein 0.3 part of n-hexane, 0.7 part of petroleum ether, and 6 parts of a uniform slow-release solvent, wherein 5 parts of (Z) -9-octadecenoic acid and 1 part of liquid paraffin oil are used; the compositions of the protective agent are as follows by mass: composition a is 1 part, wherein the benzoate of o-hydroxybenzoate is 0.25 part, the 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole is 0.25 part, the 2,4-dihydroxybenzophenone is 0.25 part, and the resorcinol monobenzoate is 0.25 part; the composition b is 2 parts, wherein: the triphosphite is 1.2 parts, and the 2,6-di-tert-butyl-p-cresol is 0.8 part. The components and mass contents of the synergist are shown in the following table, and the addition amount of the synergist is added according to the mass percentage of the related components, and is specifically shown in the following table.
Table 5: the synergist comprises the following components in percentage by mass: is based on
1-7 of locust agglutinin attractant composition according to the components and mass.
Meanwhile, when the components and contents of the locust aggregation, the slow release solvent and the protective agent are the same, the composition CK is obtained without adding a synergist.
Test site: inner Mongolia Silibin Guo Allen
Time: 7-9 months in 2021
The locust aggregation hormone attractant compositions 1 to 8 and the composition CK are respectively smeared on the same locust attracting device, each trapper is 75 meters apart, a random block design is adopted, each treatment is repeated for five times, continuous investigation is carried out, a lasting period (the lasting period refers to the accumulated days capable of attracting target pests) is recorded, the number of attracted target pests is recorded in the morning, and the statistics by taking the average number of the five repetitions of the composition CK and the composition 1 to 7 as a unit are as follows: the number and daily average insect trapping amount of the trapped locusts are as follows:
table 6: locust trapping effect analysis of locust agglutinin attractant composition
From the above table, it can be seen that: the locust aggregation element attractant compositions 1 to 7 and the composition CK can continuously induce the locust within 60 days of the test, but the number of the locust and the daily average attracting amount of the locust aggregation element attractant compositions 1 to 7 and the composition CK in the trapping set have great difference. Wherein, the locust aggregation element attractant composition 1-7 has 367-435 heads, and the daily insect trapping amount is 6.12-7.25 heads. Therefore, the insect attracting effect can be better achieved by adding any one of the synergists, and the influence of adding one or more of the synergists on the total insect attracting amount is small.
Compared with the locust aggregation element attractant compositions 1 to 7, the composition CK with no synergist component and the same other components has the total insect trapping amount of 189 heads and is only 0.43 to 0.52 of the combination groups 1 to 7.
In conclusion, the addition of the synergist can enable the locust aggregation pheromone composition to play a larger trapping role, so that the locust aggregation pheromone composition has obvious advantages in the aspects of locust number trapping, daily average trapping amount, product duration and stability of target pests.
Example 4:
the locust agglutinin attractant composition comprises the following components in percentage by mass: 12% of locust agglutinin, 85% of slow release solvent, 1.5% of synergist and 1.5% of protective agent; wherein, the locust agglutinin comprises the following components: 0.9 part of guaiacol, 8 parts of phenol, 0.9 part of trans-2-pentanal, 3 parts of hexanal, 2.9 parts of cis-3-hexenol, 1.3 parts of benzyl alcohol, 2.7 parts of alpha-methyl-beta-ethylacrolein, 2.5 parts of 2-octanone, 1 part of 2-heptanone and 4.1 parts of 2,5-dimethylpyrazine; the slow release solvent comprises the following relevant components in percentage by mass: 1.2 parts of a quick slow-release solvent, wherein 0.3 part of n-hexane, 0.9 part of petroleum ether, and 8 parts of a uniform slow-release solvent, wherein 4 parts of (Z) -9-octadecenoic acid, 2 parts of liquid paraffin oil, and 2 parts of isopropyl myristate; the components and mass contents of the compositions of the protective agent are shown in the table below, and the addition amount is based on the standard mass number and is added according to the required parts of the related components.
Table 7: the protective agent comprises the following components in percentage by mass: portions are
Locust agglutinin attractant composition 1-8 comprising the above components by weight
Meanwhile, under the condition that the components and the contents of the locust aggrecan, the slow release solvent and the synergist are the same, the locust aggrecan, the slow release solvent and the synergist are not added, and the locust aggrecan, the slow release solvent and the synergist are used as a composition CK;
test site: jiangling in Hubei province
Time: 7-9 months in 2021
Adding the locust aggregation hormone attractant compositions 1-8 and the composition CK on a polyisoprene carrier, arranging on the same locust attracting device, spacing 100 meters between every two traps, adopting a random block design, repeating every treatment for five times, continuously investigating, recording a lasting period (the lasting period refers to accumulated days capable of attracting target pests), recording the number of attracted locusts and the number of parasitic insects in the morning every day, and counting by taking the average of five repetitions of the composition CK and the composition 1-8 as a unit: the number of the trapped locust, the accumulated duration of the attractant, the anti-interference capacity of the environment and the field applicability are as follows:
table 8: locust trapping effect and carrier appearance record of locust agglutinin attractant composition
From the above table, it can be seen that: the locust agglutinin attractant compositions 1-6 have a lasting period of over 60 days, a total insect-trapping amount of 371-457 heads and a daily average insect-trapping amount of 6-7 heads, no obvious difference exists between the compositions 1-6, and the appearance of the carrier is basically intact.
Compared with the locust aggregation attractant compositions 1 to 6, the composition 7 without the protective agent composition a and the composition 8 without the protective agent composition b, the effective period and the total insect trapping amount of the locust aggregation attractant compositions are obviously reduced, the effective period is only 27 to 29 days, and is less than 1/2 of the effective period of the locust aggregation attractant compositions 1 to 6. The total insect-trapping amount is only 108-120, which is less than 1/3 of the total insect-trapping amount of the locust agglutinin attractant composition 1-6, and the carrier has slight deterioration, discoloration or swelling phenomenon.
However, the composition CK with the same components and without the synergist has the shortest duration of only 20 days, less total insect trapping amount of only 55 heads, and the carrier is deteriorated and has the phenomena of color change and swelling.
In conclusion, the protective agent can absorb ultraviolet rays, stabilize illumination and reduce rainwater impact, so that the aggregation pheromone is released at a constant speed according to a certain proportion, the duration is prolonged, the trapping effect is improved, the effective components and the carrier are released more stably, the duration is prolonged, the trapping effect is better, the insect trapping amount is more, and the inhibiting effect of the improper content of the protective agent on the effective components of the aggregation pheromone can be avoided.
Example 5:
the locust agglutinin attractant composition comprises the following components in percentage by mass: 10.0 percent of locust agglutinin, 86 percent of slow release solvent, 2.5 percent of synergist and 1.5 percent of protective agent; wherein, the locust agglutinin comprises the following components by mass: 0.5 part of guaiacol, 4 parts of phenol, 1.5 parts of trans-2-pentanal, 3 parts of hexanal, 2.5 parts of cis-3-hexenol, 1 part of benzyl alcohol, 4 parts of alpha-methyl-beta-ethylacrolein, 3 parts of 2-octanone, 2 parts of 2-heptanone and 4 parts of 2,5-dimethylpyrazine; the slow-release solvent comprises the following components in parts by mass: the quick slow-release solvent is 1.5 parts: wherein, the n-hexane is 0.7 part, the petroleum ether is 0.8 part, the uniform slow-release solvent is 8.5 parts, the (Z) -9-octadecenoic acid (oleic acid) is 5.1 parts, the liquid paraffin oil is 1.8 parts, and the isopropyl myristate is 1.6 parts; the synergist is a mixture of leaf alcohol and benzaldehyde; the compositions of the protective agent are as follows by mass: the composition a is 1.1 parts, wherein 0.5 part of phenyl ortho-hydroxybenzoate and 0.6 part of 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole are used; the composition b is 1.8 parts, wherein 0.2 part of triphosphite, 0.1 part of vitamin E and 1.5 parts of 2,6-di-tert-butyl-p-cresol are used as the locust aggregation attractant composition, and a butyl rubber head is used as a carrier.
Optionally matching with locust agglutinin luring cores 1 and 2 of the prior art to perform multi-effect control test
Test site: inner Mongolia Xilinguo, yunnan Mongolia and Hebei Cangzhou yellow Ye south-Dagang wetland
Time: 7-9 months in 2021
Placing the locust aggregation element attractant composition and the prior art on the same locust attracting device, wherein each trapper is spaced by 100 meters, adopting a random block design, repeating each treatment for five times, continuously investigating, recording a duration (the duration refers to the accumulated days for attracting target pests), recording the number of attracted target pests and the number of mixed pests in the morning of each day, and counting by taking the average number of five repetitions of the composition CK and the composition 1-9 as a unit: the population number of the lured locust, the accumulated duration of the attractant, the anti-interference capacity of the environment and the field applicability are as follows:
table 9: the invention and the prior art analyze the locust trapping effect
From the above table, it can be seen that:
compared with the prior art, the invention has remarkable advantages in lasting period, locust number, hybrid rate, male-female ratio and locust nymph trapping ratio in various tests, the lasting period can reach 66-82 days, the locust number is 462-502, and the trapped locust comprises: locusts migratorius, locusts swordsmanniana, locusts and the like, wherein the rate of the undesired insects is 5.46-6.29%, the locusts nymphs account for 38.49-42.83%, and the male-female ratio is 1.20-1.46.
Whereas the trapping duration period of the prior art 1 is: the number of locusts is 210-225 in 40-44 days, the rate of the undesired insects is 20.15-22.18%, the ratio of locusts nymphs is 9.50-18.57%, and the ratio of male to female is 0.52-0.63.
The trapping duration period of the prior art 2 is: the number of locusts is 115-206 in 24-30 days, the rate of the undesired insects is 15.23-25.32%, the ratio of locusts nymphs is 13.59-13.91%, and the ratio of male and female is 0.58-0.64.
Thus, it can be seen that: the prior art 1 and the prior art 2 have obvious advantages in the aspects of the induction and collection duration, the locust number, the rate of the undesired insects, the ratio of the locust nymphs and the male-female ratio, although the duration and the index of the locust number of the prior art 1 are superior to those of the prior art 2, compared with the invention, the duration and the total insect trapping amount have obvious differences, the duration is only 40-44 days, the total insect trapping amount is 210-225, and is only 1/2 of the total insect trapping amount.
Meanwhile, in the whole-period locust trapping test, the conditions of rain, dryness and high-intensity solarization are existed, and the locust trapping device still has good lasting period and trapping effect.
In conclusion, the locust-killing method has the characteristics of long lasting period, large amount of trapped insects, strong specificity, high stability and the like, is suitable for trapping and killing locusts in more regions, more types and more instars, and can meet the requirement of accurate and stable monitoring of field target pests. The above description is not meant to be limiting, it being noted that: it will be apparent to those skilled in the art that various changes, modifications, additions, substitutions and limited experimentation can be made without departing from the spirit and scope of the invention.
Claims (10)
1. An acridid agglutinin attractant composition, comprising: locust agglutinin, slow release solvent, synergist and protectant; the method is characterized in that: the locust agglutinin attractant composition comprises, by mass, 5-12% of locust agglutinin, 85-92% of slow release solvent, 0.9-3.6% of synergist and 0.8-2.2% of protective agent.
2. The locust lectin attractant composition of claim 1, wherein: the locust agglutinin comprises the following components: guaiacol, phenol, trans-2-pentanal, hexanal, cis-3-hexenol, benzyl alcohol, alpha-methyl-beta-ethylacrolein, 2-octanone, 2-heptanone and 2,5-dimethylpyrazine.
3. The locust lectin attractant composition of claim 2, wherein: the locust agglutinin comprises the following components in parts by mass: 0.2 to 2 portions of guaiacol, 1 to 10 portions of phenol, 0.5 to 2 portions of trans-2-pentanal, 1 to 4 portions of hexanal, 1 to 3 portions of cis-3-hexenol, 1 to 2 portions of benzyl alcohol, 0.5 to 10 portions of alpha-methyl-beta-ethylacrolein, 0.5 to 4 portions of 2-octanone, 0.5 to 4 portions of 2-heptanone and 1 to 5 portions of 2,5-dimethylpyrazine.
4. The locust lectin attractant composition of claim 1, wherein: the slow release solvent comprises a quick slow release solvent and a uniform slow release solvent, wherein the quick dilution solvent is one or a combination of n-hexane and petroleum ether; the uniform speed sustained-release solvent is one or more of (Z) -9-octadecenoic acid (oleic acid), liquid paraffin oil and isopropyl myristate.
5. The locust lectin attractant composition of claim 4, wherein: the slow release solvent comprises the following components in percentage by mass: 1-1.5 parts of quick dilution solvent and 6-9 parts of uniform speed slow release solvent.
6. The locust lectin attractant composition of claim 1, wherein: the synergist is one or more of leaf alcohol, benzaldehyde and wheat bran essence.
7. The locust agglutinin attractant composition of claim 1, wherein: the protective agent comprises two compositions, wherein the composition a is: one or more of phenyl o-hydroxybenzoate, 2- (2 '-hydroxy-5' -methylphenyl) benzotriazole, 2,4-dihydroxybenzophenone, and resorcinol monobenzoate; the composition b is one or more of triphosphite, vitamin E, 2,6-di-tert-butyl-p-cresol.
8. The locust lectin attractant composition of claim 4, wherein: the protective agent comprises 1-1.5 parts of a composition a and 1-3 parts of a composition b by mass.
9. The locust lectin attractant composition of claim 1, wherein: the composition can be used for locusts migratoria, locusts, locusts canadensis and locusts rhombus, gathering and luring locusts of various locusts.
10. The locust lectin attractant composition of claim 1, wherein: the composition can be singly smeared or matched with a rubber plug or matched with a locust attracting device for use.
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