CN115804380A

CN115804380A - Pesticide sterilization composition containing metalaxyl-M and application thereof

Info

Publication number: CN115804380A
Application number: CN202211272667.8A
Authority: CN
Inventors: 陈长军; 叶文武; 王光; 张龙; 石伟山; 王源超; 张申伟
Original assignee: Jiangsu Aijin Crop Technology Group Co ltd; Nanjing Agricultural University
Current assignee: Jiangsu Aijin Crop Technology Group Co ltd; Nanjing Agricultural University
Priority date: 2022-10-18
Filing date: 2022-10-18
Publication date: 2023-03-17

Abstract

The invention discloses a pesticide sterilization composition containing metalaxyl-M and application thereof, relating to the field of pesticide preparations. In the invention, fluazinam, fludioxonil and metalaxyl-M are compounded to serve as active ingredients of the pesticide preparation, effective sterilization can be realized through three different sterilization mechanisms, the generation of drug resistance of bacteria and fungi is delayed, and the synergistic effect of the three substances shows an additive effect. The pesticide preparation prepared by mixing the pesticide preparation with a specific wetting dispersant, a film forming agent, a thickening agent, a warning color, an antifreezing agent, a defoaming agent, a preservative and water has broad-spectrum bactericidal property, good suspension stability and slow-release effect, high and uniform film forming speed, strong adhesion with the surface of seeds, low shedding rate and high use value, and can be widely used for killing various diseases of crops such as soybean, wheat, corn, peanut, potato, rice and the like.

Description

Pesticide sterilization composition containing metalaxyl-M and application thereof

Technical Field

The invention relates to the field of pesticide preparations, in particular to a pesticide sterilization composition containing metalaxyl-M and application thereof, and belongs to the field of pesticide preparations A01P 3/00.

Background

The root is an important organ for plants to absorb water, nutrients and inorganic salts in the environment, and not only transports nutrient components required by the growth of the plants to the stem and the leaf to promote the growth of the plants, but also can store energy to ensure that the crops can stably root and survive in soil. If the roots are attacked by pathogenic bacteria, the roots are damaged, and the normal growth of crops is affected and even the crops die. Wherein, the root rot is mainly caused by the infection of phytophthora (oomycetes) and fusarium (fungi). Root rot can cause rot of plant roots, further cause dwarfing or death of plants, has a wide host range, and can damage various crops such as wheat, corn, soybean, peanut and the like. In addition, the bakanae disease of rice belongs to a fungal pest disease, a germ-carrying seed is a main primary infection source of the disease, germs are attached to the surface of the seed through conidium or are latent in the seed through mycelium, and when the seed germinates, the germs invade seedlings from coleoptiles to cause the disease attack. At present, the influence of crop diseases is removed, the use of chemical pesticide agents becomes the optimal choice, but with the increase of the application time or the dosage of the agents, the disease fungi can be caused to have resistance, so that the pesticide effect of the pesticide preparation is reduced and even inactivated, and the environment is polluted.

Chinese patent application CN110367251A discloses a synergistic pesticide composition containing beta-pinene, which comprises an active component A and an active component B, wherein the active component A is the beta-pinene, the active component B is at least one selected from fluazinam, metalaxyl-M fludioxonil and the like, and the beta-pinene can promote the active components to enter an active body, so that the synergistic pesticide composition is favorable for better exerting the effect of chemical pesticides and can increase the prevention effect on harmful organisms, reduce the use of chemical pesticide amount and reduce the cost. Chinese patent application CN109105375A discloses a high-efficiency comprehensive pesticide-fertilizer application prevention and control method for reducing incidence rate of continuous cropping obstacles of peppers, wherein metalaxyl-M and fludioxonil are used for the first time and fluazinam is used for the fourth time in four pesticide application processes after base fertilizer application, finally, the acre yield is improved by 26.3 percent compared with the acre yield of a conventional control scheme of a farmer, the incidence rate of pests and diseases related to the continuous cropping obstacles of peppers is reduced by 20 percent, and the yield increase effect is obvious. However, fluazinam has no resistance, but has poor mixing property with other substances, and no relevant research on the treatment of crop diseases such as corn, wheat and the like by compounding three active ingredients, namely fluazinam, fludioxonil and metalaxyl-M is found.

Disclosure of Invention

In order to solve the above problems, the present invention provides a pesticide sterilization composition containing metalaxyl-M, the active ingredients of which are fluazinam, fludioxonil and metalaxyl-M.

Fluazinam

Fluazinam, the chemical name of which is 3-chloro-N- (3-chloro-5-trifluoromethyl-2-pyridyl) -a, a, a-trifluoro-2, 6-dinitro-p-toluidine and the molecular formula of which is C ₁₃ H ₄ C _l2 F ₆ N ₄ O ₄ The bactericidal composition is a dinitroaniline bactericide, has no treatment effect and systemic activity, and belongs to a broad-spectrum efficient preventive protective bactericide. The beta-trifluoromethyl aminopyridine acts on a plurality of specific sites of ATP synthetase to release the correlation of oxidation and phosphorylation at the tail end of a respiratory chain, consumes electrochemical potential energy accumulated by electron transfer to the maximum extent, blocks the formation of germ energy (ATP), and inhibits infectionThe germination, penetration, hyphal growth and spore formation of the pathogen spores during the process, resulting in the death of the pathogen. The fluazinam has high activity, small dosage, quick response, no resistance, no cross resistance with other medicaments, and good control effect on pathogenic bacteria generating drug resistance. However, fluazinam has poor stability, is easy to oxidize, has strong permeability, is easy to cause phytotoxicity, and has great limitation in the aspect of mixing property.

Fludioxonil

Fludioxonil, having the chemical name of 4- (2, 2-difluoro-1, 3-benzodioxy-4-yl) pyrrole-3-carbonitrile and the molecular formula of C ₁₂ H ₆ F ₂ N ₂ O ₂ The bactericide is also called fludioxonil, fludioxonil and aptanle, is a phenylpyrrole bactericide, belongs to a contact type bactericide, mainly inhibits the growth of fungal mycelia by inhibiting the transfer related to glucose phosphorylation, destroys the synthesis of nucleic acid and protein to express the sterilization and bacteriostasis effects, has the advantages of good control effect, safety, low toxicity, long duration, good tolerance and the like, has no cross resistance with the existing bactericide, but has no systemic property, and is easy to cause the serious resistance of germs when being applied alone.

metalaxyl-M

metalaxyl-M, chemical name is N- (2, 6-xylyl) -N- (methoxyacetyl) -D-alanine methyl ester, molecular formula is C ₁₅ H ₂₀ O ₄ N, also called as high-efficiency metalaxyl, is an anilide bactericide, belongs to a bactericide with three-dimensional optical activity, has strong systemic property, and achieves the bactericidal effect mainly by inhibiting the activity of ribosomal RNA polymerase. metalaxyl-M has high efficiency and broad spectrum, is safe and low in toxicity, has low residue, long lasting period and diversified application modes, and simultaneously has protection and treatment effects. But the single use of the compound is easy to generate drug resistance and the compound is easy to decompose and lose efficacy.

In some preferred embodiments, the weight ratio of fluazinam, fludioxonil and metalaxyl-M is (1-12): (1-13): (1-8); preferably (1 to 10): (1-10): (1-5); more preferably 2.5:2.5:1.

in the invention, fluazinam, fludioxonil and metalaxyl-M are mixed and used as the sterilization composition, the combination of the fluazinam, fludioxonil and metalaxyl-M does not cause the reduction of toxicity among the fluazinam, and the sterilization composition can inhibit the reproduction and growth of germs and fungi in many aspects due to different specific sterilization mechanisms, thereby effectively enhancing the sterilization effect, expanding the sterilization range and reducing the use probability of various medicaments to a certain extent. Particularly, when the weight ratio of fluazinam to fludioxonil to metalaxyl-M is (1-12): (1-13): (1-8), particularly 2.5:2.5:1, the addition effect is remarkable. The composition has proper permeability on the surface of the seeds, high activity, and is not easy to decompose and oxidize, and the composition has the function of sterilization without influencing the normal germination and growth of the seeds. In addition, the synergistic effect of different sterilization modes further relieves the generation of cross resistance, reduces the using amount of the pesticide sterilization composition and further reduces the pollution to the environment.

The invention provides an application of a pesticide sterilization composition containing metalaxyl-M in preparing pesticide preparations.

In some preferred embodiments, the pesticide preparation is in a dosage form selected from any one of dispersible oil suspending agent, seed treatment suspending agent, microcapsule suspending agent, microemulsion, granule, aqueous emulsion, wettable powder and water dispersible granule; preferably a seed treatment suspending agent.

In some preferred embodiments, the concentration of the pesticide sterilization composition containing metalaxyl-M in the pesticide preparation is 5-30% by weight; preferably 15%.

In some preferred embodiments, the pesticide formulation further comprises: wetting dispersant, film forming agent, thickening agent, warning color, antifreezing agent, defoaming agent, preservative and water.

In some preferred embodiments, the pesticide preparation further comprises 1-7.5% of wetting dispersant, 0.5-5% of film forming agent, 0.1-2.5% of thickening agent, 1.5-6.5% of warning color, 0.05-4% of antifreezing agent, 0.2-0.8% of defoaming agent, 0.005-0.5% of preservative and water added to 100% in percentage by weight.

In some preferred embodiments, the wetting and dispersing agent is selected from at least one of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkyl glycoside, fatty amine polyoxyethylene ether, lignosulfonate, alkylphenol resin polyoxyethylene ether sulfate, methyl naphthalene sulfonic acid formaldehyde condensate sulfate, aryl polyoxyalkyl sulfate, trisiloxane polyoxyethylene ether, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate and castor oil polyoxyethylene ether; preferably a combination of alkylphenol ethoxylates, lignosulfonates and aryl polyoxyalkyl sulfates; further preferably a combination of octylphenol polyoxyethylene ether, sodium lignin sulfonate and ammonium styrene phenol polyoxyethylene ether sulfate.

In some preferred embodiments, the weight ratio of the octylphenol polyoxyethylene ether, lignosulfonate, and aryl polyoxyalkyl sulfate is 1: (0.45-0.9): (0.2-0.75); preferably 1:0.75:0.5.

in some preferred embodiments, the octylphenol polyoxyethylene ether has a hydroxyl value of 80 to 100mgKOH/g, an HLB value of 12 to 15; preferably, the hydroxyl value of the octyl phenol polyoxyethylene ether is 87 +/-5 mgKOH/g, and the HLB value is 13.3-14.

In some preferred embodiments, the sulfonic acid group in the sodium lignosulfonate may be located on any one of an aliphatic chain, a benzene ring, a mixture of an aliphatic chain and a benzene ring; preferably on a benzene ring.

In some preferred embodiments, the sodium lignosulfonate with the sulfonic acid group on the benzene ring has a sulfonation degree of 0.7-2.0, an average molecular weight of 8500-11000, and a viscosity of 70-90mPa · s; preferably, the sodium lignosulfonate with the sulfonic acid group on the benzene ring has the sulfonation degree of 0.8, the average molecular weight of 10000 and the viscosity of 86mPa & s.

In some preferred embodiments, the pH value of the styrene phenol polyoxyethylene ether ammonium sulfate in a 1% (v/v) aqueous solution is 4-7, and the active content is more than or equal to 98.5wt%; preferably, the pH value in a 1% (v/v) aqueous solution is 4-6 and the active content is 99wt% or more.

The combination of alkylphenol polyoxyethylene, lignosulfonate and aryl polyoxyalkyl sulfate is added into the system, particularly the combination of octyl phenol polyoxyethylene, sodium lignosulfonate and styrene phenol polyoxyethylene ammonium sulfate is selected, so that the dispersion stability of the active ingredients in the system can be improved. Probably, the addition of the octyl phenol polyoxyethylene ether, the sodium lignosulfonate and the styrene phenol polyoxyethylene ether ammonium sulfate is favorable for reducing the surface tension of the pesticide active ingredient and other components, the sodium lignosulfonate contains an anchoring structure of sulfonate anions and can be better anchored and combined with other components in a system, and the sulfonic acid group is positioned on a benzene ring, so that a certain steric hindrance effect can be generated, the pesticide active ingredient is surrounded by more solvents, the dispersion speed and the dispersion degree of the active ingredient in the system are increased, the suspension rate and the suspension stability of the pesticide preparation are improved, and the slow release property and the temperature resistance stability of the pesticide preparation are enhanced.

In some preferred embodiments, the film forming agent is selected from at least one of polyvinyl alcohol, chitosan, polyvinyl acetate, carboxymethyl cellulose, xanthan gum, acrylic resin, urethane copolymer resin, acrylate modified butadiene resin, urethane modified nitrocellulose, and attapulgite; polyvinyl alcohol and xanthan gum are preferred.

In some preferred embodiments, the weight ratio of polyvinyl alcohol to xanthan gum is 1: (1.3-2.2); preferably 1:1.8.

in some preferred embodiments, the polyvinyl alcohol has an alcoholysis degree of 80 to 95% and a viscosity at 25 ℃ of 15 to 30mPa · s; preferably, the degree of alcoholysis is 87.0 to 89.0% and the viscosity at 25 ℃ is 31.0 to 39.0 mPas.

In the invention, the film-forming property of the pesticide bactericidal composition on the surface of the seed can be promoted by adding the film-forming agent into the system, but certain strength and permeability are also required. The inventor unexpectedly finds that when polyvinyl alcohol and xanthan gum are selected as film forming agents of pesticide preparations, the polyvinyl alcohol contains a large amount of hydroxyl, and the xanthan gum is a polysaccharide high molecular polymer product containing long-chain branched chains and has a special net structure, and the synergistic effect of the polyvinyl alcohol and the xanthan gum promotes the pesticide preparations to quickly form films on the surfaces of seeds. Meanwhile, a honeycomb-like structure can be formed, the active ingredients and other ingredients are supported, and the suspension stability of the system is further promoted. In particular when the weight ratio of polyvinyl alcohol to xanthan gum is 1: (1.3-2.2) the agricultural chemical preparation is solidified on the surface of the seed to form a film. The active ingredients can be bound not to fall off, and meanwhile, the active ingredients can absorb water and swell in soil without dissolving, so that the water and air circulation required by the growth of seeds is ensured, the slow release of the drug effect of the active ingredients is realized, and the temperature resistance stability of the pesticide preparation can be improved.

In some preferred embodiments, the thickening agent is selected from at least one of starch, sodium starch phosphate, gum arabic, pectin, agar, gelatin, alginate jelly, carrageenan, dextrin, sodium carboxymethylcellulose, propylene glycol alginate, methylcellulose, sodium alginate, casein, polyvinylpyrrolidone; preferably starch and dextrin.

In some preferred embodiments, the weight ratio of starch to dextrin is 1: (0.7-1.5); preferably 1:1.2.

in some preferred embodiments, the starch is selected from at least one of corn starch, sweet potato starch, modified starch; preferably corn starch.

In some preferred embodiments, the dextrin is selected from at least one of a-cyclodextrin, β -cyclodextrin, γ -cyclodextrin, branched cyclodextrin; beta-cyclodextrin is preferred.

In some preferred embodiments, the anti-freeze agent is selected from at least one of isopropyl alcohol, ethylene glycol, propylene glycol butyl ether, ethylene glycol butyl ether acetate, formamide, calcium chloride, sodium acetate, urea; ethylene glycol and urea are preferred.

In some preferred embodiments, the weight ratio of ethylene glycol to urea is 1: (0.8-2); preferably 1:1.4.

in some preferred embodiments, the antifoaming agent is selected from at least one of polyglycerol, lauric acid, isooctanol, stearic acid, epoxidized soybean oil, polyoxypropylene glycerol ether, silicone oil, polyethylene glycol ether, polyether modified silicon; preferred are silicone and polyether modified silicon.

In some preferred embodiments, the weight ratio of the silicone to the polyether modified silicon is 1: (0.5-1.5); preferably 1:1.

in some preferred embodiments, the silicone has a viscosity of 2000 to 6500mPa · s at 25 ℃ and a solids content of 25 to 40wt%; preferably, the viscosity at 25 ℃ is 3000 to 5500 mPas and the solids content is 29 to 31wt%.

In some preferred embodiments, the polyether-modified silicon has an effective substance content of 97 to 99.5wt% and a pH of 5 to 9; preferably, the content of active substance is 98wt% and the pH is 5-8.

The warning color and the preservative are not particularly limited in the present application.

In some preferred embodiments, the warning color is selected from at least one of aqueous rose bengal, acid scarlet, permanent RED FGR, FGR-131, RED 8110; preferably permanent red FGR.

In some preferred embodiments, the preservative is selected from at least one of formaldehyde, benzoic acid, sodium benzoate, benzaldehyde, potassium benzoate, phenyl salicylate, sorbic acid, sodium salicylate, 2 hydroxybiphenyl, p-hydroxybenzaldehyde, and 1,2 benzisothiazoline 3 one; phenyl salicylate is preferred.

In some preferred embodiments, the method of preparing the pesticide formulation comprises: mixing the active ingredient, the wetting dispersant defoaming agent and water, sanding, adding the rest raw materials, and mixing to obtain the final product.

Compared with the prior art, the invention has the following beneficial effects:

(1) According to the invention, fluazinam, fludioxonil and metalaxyl-M are compounded to serve as active ingredients of the pesticide preparation, and three different sterilization mechanisms are adopted, so that the pesticide preparation can be effectively sterilized, the generation of drug resistance of bacteria and fungi is delayed, and the three substances are synergistic to show an obvious addition effect and have excellent sterilization effects on phytophthora sojae, pythium aphanidermatum (such as pythium spinulosum), fusarium graminearum, fusarium, phomopsis solani, rhizoctonia solani, sclerotinia sclerotiorum, anthracnose and the like.

(2) According to the invention, the active ingredient compounded by fluazinam, fludioxonil and metalaxyl-M is mixed with the specific wetting dispersant, the film-forming agent, the thickening agent, the warning color, the antifreezing agent, the defoaming agent, the preservative and the water, so that the prepared pesticide preparation not only has broad-spectrum bactericidal property, but also has good suspension stability, and the active ingredient shows a certain slow release effect. The pesticide preparation has the advantages of high and uniform film forming speed, strong adhesion with the surface of seeds, low shedding rate and use value, can be widely used for killing various diseases of crops such as soybean, wheat, corn, peanut, potato, rice and the like, has a relative prevention effect of 76.3 percent, and reduces the morbidity by 46.2 percent.

Detailed Description

Determination of combined toxicity effect of fluazinam, fludioxonil and metalaxyl-M on Pythium ultimum by mixing

Test agents and media

95.5% fludioxonil original drug is provided by Hangzhou Yulong chemical Co., ltd; the 96% fluazinam technical material is provided by Zhejiang chemical research institute Co., ltd; 98% metalaxyl-M technical is provided by Zhejiang Yifan chemical Limited. Respectively dissolving fludioxonil, fluazinam and metalaxyl-M in methanol to obtain 10 ⁴ Dissolving salicylic acid in methanol to obtain 5.0 × 10 μ g/mL mother liquid ⁴ mu.g/mL of the mother liquor was stored in a refrigerator at 4 ℃.

V8 medium: 200g of vegetable juice of U.S. import V8 ₃ 2g, 16g of agar powder and 1000mL of water;

the culture medium is sterilized at high temperature and high pressure before use, and then stored at normal temperature.

Fresh bacteria dishes at the edges of bacterial colonies cultured on a V8 culture medium for 2 days are respectively inoculated into solutions prepared from 1000 mu g/mL fluazinam, fludioxonil and metalaxyl-M mother liquor, and the culture media with different volume ratios and different formula ratios are designed according to the formula ratios and the concentrations thereof (shown in the following table 1), the combined toxicity effect of the bacteria on the pythium ultimum is measured, and the specific measurement results are shown in the table 2.

TABLE 1 concentration of fluazinam, fludioxonil and metalaxyl-M different concentration design

TABLE 2 measurement results of the combined toxicity of fluazinam, fludioxonil and metalaxyl-M at different concentrations on Pythium ultimum

^A EC ₅₀ : effective concentration to inhibit 50% hyphal growth; ^B MIC: the minimum inhibitory concentration; the C value in the upper right corner after each column of data represents no significant difference after the same treatment (P = 0.05.).

The MIC values of fluazinam and metalaxyl-M on Pythium ultimum are as high as 300 mu g/mL and 400 mu g/mL, and the ratio of the fluazinam to the metalaxyl-M is 1:1: at 10, is EC in all ratios ₅₀ The lowest value ratio was 0.413. Mu.g/mL, SR (EC) ₅₀ ) 1.12, the additive effect is shown, and the MIC value is reduced by 100 mu g/mL compared with that of a single fluazinam agent. With the decrease of metalaxyl-M and the increase of fluazinam in the proportion, the whole EC ₅₀ Value rise MIC value fall. In the ratio of 5:5:2, EC50 value of 1.34 μ g/mL, SR (EC 50) equal to 1.05, showed additive effect, SR (MIC) 3.23, thus determining the ratio fluazinam: fludioxonil: metalaxyl-M5: 5: and 2, screening the optimal formula for the candidate proportion.

Example 1

1. A pesticide sterilization composition containing metalaxyl-M comprises fluazinam, fludioxonil and metalaxyl-M as active ingredients.

The weight ratio of fluazinam to fludioxonil to metalaxyl-M is 2.5:2.5:1.

2. an application of a pesticide sterilization composition containing metalaxyl-M in preparing pesticide preparations.

The pesticide preparation is in the dosage form of a seed treatment suspending agent.

The mass percentage concentration of the pesticide sterilization composition containing metalaxyl-M in a pesticide preparation is 15%.

The pesticide formulation further comprises: 4.5% of wetting dispersant, 1.5% of film forming agent, 1% of thickening agent, 3% of warning color, 0.5% of antifreezing agent, 0.3% of defoaming agent, 0.005% of preservative and 100% of water.

The wetting dispersant is the combination of octyl phenol polyoxyethylene ether, sodium lignosulfonate and styrene phenol polyoxyethylene ether ammonium sulfate.

The weight ratio of the octyl phenol polyoxyethylene ether to the lignosulfonate to the aryl polyoxyalkyl sulfate is 1:0.75:0.5.

the hydroxyl value of the octyl phenol polyoxyethylene ether is 87 +/-5 mgKOH/g, and the HLB value is 13.3-14 (Ohio petrochemical plant, OP-10, jiangsu province).

The sulfonic group in the sodium lignosulphonate is positioned on a benzene ring.

The sulfonation degree of the sodium lignosulfonate with the sulfonic acid group positioned on the benzene ring is 0.8, the average molecular weight is 10000, and the viscosity is 86mPa & s (Reax 85A).

The pH value of the styrene phenol polyoxyethylene ether ammonium sulfate in a 1% (v/v) aqueous solution is 4-6, and the active matter content is more than or equal to 99wt% (XPO-5, haian petrochemical plant of Jiangsu province).

The film forming agent is polyvinyl alcohol and xanthan gum.

The weight ratio of the polyvinyl alcohol to the xanthan gum is 1:1.8.

the alcoholysis degree of the polyvinyl alcohol is 87.0-89.0%, and the viscosity at 25 ℃ is 31.0-39.0 mPa.s (medium-petrochemical polyvinyl alcohol 2088).

The xanthan gum was purchased from warrior technologies development limited, martian.

The thickening agent is starch and dextrin.

The weight ratio of the starch to the dextrin is 1:1.2.

the starch is corn starch (Shaanxi Tangyao Biotech Co., ltd.).

The dextrin is beta-cyclodextrin.

The antifreezing agent is ethylene glycol and urea.

The weight ratio of the ethylene glycol to the urea is 1:1.4.

the defoaming agent is organic silicon and polyether modified silicon.

The weight ratio of the organic silicon to the polyether modified silicon is 1:1.

the viscosity of the organic silicon AT 25 ℃ is 3000-5500 mPa s, and the solid content is 29-31 wt% (field chemistry, AT-930).

The polyether modified silicon has an active substance content of 98wt% and a pH of 5 to 8 (Wan New Material Co., ltd., guangzhou City).

The warning color is permanent red FGR.

The preservative is phenyl salicylate.

The preparation method of the pesticide preparation comprises the following steps: mixing the active ingredients, the wetting dispersant, the defoaming agent and water, sanding, adding the rest raw materials, and mixing to obtain the final product.

Example 2

1. The pesticide sterilization composition containing metalaxyl-M is the same as the pesticide sterilization composition in the example 1.

2. An application of a pesticide sterilization composition containing metalaxyl-M in preparing pesticide preparations. The difference from example 1 is that:

the pesticide formulation further comprises: 5% of wetting dispersant, 1.3% of film forming agent, 1.4% of thickening agent, 3.5% of warning color, 0.5% of antifreezing agent, 0.35% of defoaming agent, 0.005% of preservative and 100% of water.

Comparative example 1

the sodium lignosulfonate having the sulfonic acid group on the benzene ring had a sulfonation degree of 1.8, an average molecular weight of 9000, and a viscosity of 29mPa · s (readx 83A).

Comparative example 2

2. An application of a pesticide sterilization composition containing metalaxyl-M is used for preparing a pesticide preparation. The difference from example 1 is that:

the weight ratio of the polyvinyl alcohol to the xanthan gum is 1:3.

comparative example 3

1. The pesticide sterilization composition containing metalaxyl-M is the same as that in example 1.

the weight ratio of the octyl phenol polyoxyethylene ether to the lignosulfonate to the aryl polyoxyalkyl sulfate is 1:1.3:0.5.

performance testing

1. Stability: the samples obtained in the examples were tested with reference to 4.13 of the national standard GB/T17768-1999, which is shown in Table 3.

2. Suspension percentage: the samples obtained in the examples were tested with reference to 4.6 of the national standard GB/T17768-1999, which is shown in Table 3.

Table 3 results of performance testing of examples

3. Test of combined toxicity effect of fluazinam, fludioxonil and metalaxyl-M on various germs by mixing

(1) The combined toxicity test of the soybean phytophthora and the specific test result are shown in table 4.

TABLE 4 Combined virulence test results of fluazinam, fludioxonil and metalaxyl-M mixed on phytophthora sojae

EC of metalaxyl-M on soybean phytophthora ₅₀ The lowest value was 0.110. Mu.g/mL, but the MIC value was up to 400. Mu.g/mL. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2 hour, EC ₅₀ The lowest value was 0.376. Mu.g/mL, the MIC value was 60. Mu.g/mL, SR (EC) ₅₀ ) The value 1.01 is additive.

(2) The joint toxicity test of the pythium spinosum has the specific test results shown in table 5.

TABLE 5 Combined virulence test results for Pythium spinosum, fluazinam, fludioxonil and metalaxyl-M

EC of metalaxyl-M on Pythium spinosum ₅₀ The minimum value is 0.110 mu g/mL, and the minimum MIC value is 10 mu g/mL, which shows that metalaxyl-M can well inhibit the growth of the hypha of pythium spinosum. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2 hour, EC ₅₀ The value was as low as 0.425. Mu.g/mL, the MIC value was 10. Mu.g/mL, SR (EC) ₅₀ ) The value 0.82 is additive.

(3) The combined toxicity of the red shell bacterium ilex is tested, and the specific test result is shown in the table 6.

TABLE 6 Combined virulence test results for L.Donacis when fluazinam, fludioxonil and metalaxyl-M are mixed

The fludioxonil has obvious inhibition effect on the hypha growth of the red shell bacterium of ilex purpurea, the MIC value is 5 mu g/mL, and the EC value is ₅₀ The value was 0.136. Mu.g/mL. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2 hour, EC ₅₀ The value was as low as 0.311. Mu.g/mL, the MIC value was 10. Mu.g/mL, SR (EC) ₅₀ ) The value is 0.848, which is additive.

(4) The combined virulence test for Fusarium and the specific test results are shown in Table 7.

TABLE 7 Combined virulence test results for Fusarium by blending fluazinam, fludioxonil and metalaxyl-M

The MIC value of fludioxonil to Fusarium is 5 mu g/mL at most, EC ₅₀ The value is also 0.0992. Mu.g/mL at the lowest. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2, the reduction effect on the MIC value is obvious, the MIC is reduced to 10 mu g/mL, and the EC is ₅₀ The value is as low as 0.311. Mu.g/mL, SR (EC) ₅₀ ) The value is 0.550, which is additive.

(5) The combined toxicity of phomopsis longipes is tested, and the specific test result is shown in Table 8.

TABLE 8 test results of the combined toxicity of fluazinam, fludioxonil and metalaxyl-M for Phomopsis

Fluazinam and fludioxonil have good inhibition effect on hypha growth of phomopsis. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2 hour, EC ₅₀ The lowest value is 0.0165 mu g/mL, the MIC is reduced to 0.1 mu g/mL, SR (EC) ₅₀ ) The value was 1.49 and the SR (MIC) value was 3.33, which is additive.

(6) The combined toxicity of Rhizoctonia solani is tested, and the specific test results are shown in Table 9.

TABLE 9 test results of the joint toxicity of fluazinam, fludioxonil and metalaxyl-M in combination with Rhizoctonia solani

The MIC value of fludioxonil to rhizoctonia solani is minimum 1 mu g/mL, EC ₅₀ The minimum value was 0.0490. Mu.g/mL. When the ratio of fluazinam: fludioxonil: the metalaxyl-M ratio is 5:5:2 hour, EC ₅₀ The lowest value was 0.150. Mu.g/mL, the MIC value was 2. Mu.g/mL, SR (EC) ₅₀ ) The value is 0.57, which is additive.

(7) The combined toxicity test of the sclerotinia sclerotiorum shows the specific test results in table 10.

TABLE 10 Combined virulence test results for Sclerotinia sclerotiorum with fluazinam, fludioxonil and metalaxyl-M

Fluazinam and fludioxonil have good effect of inhibiting the growth of sclerotinia sclerotiorum hyphae. When the ratio of fluazinam: fludioxonil: the ratio of metalaxyl-M is 5:5: MIC value of 0.0625. Mu.g/mL minimum, EC 2 ₅₀ The minimum value is 0.0293 mu g/mL, the SR (MIC) value is 1.88 ₅₀ ) The value was 0.993, which is additive.

(8) The combined toxicity test of anthrax bacteria, the specific test results are shown in Table 11.

TABLE 11 test results of the combined virulence effect of fluazinam, fludioxonil and metalaxyl-M on anthrax

MIC value and EC of fluazinam against colletotrichum gloeosporioides ₅₀ The lowest values, 2. Mu.g/mL and 0.0431. Mu.g/mL, respectively. Fluazinam: fludioxonil: the ratio of metalaxyl-M is 5:5:2 hour, EC ₅₀ The minimum value is 0.131. Mu.g/mL, the MIC value is 2. Mu.g/mL, SR (MIC) is 2, ₅₀ ) A value of 0.655, showing additive effect.

3. The field efficacy test report: the specific results for controlling soybean root rot are shown in table 12.

Cell division: v 224719 in Suzhou city, 10 treatments are totally divided, each treatment is repeated 4 times, and each cell is 10m ² 。

And (4) result investigation: all soybean plants within the plot were investigated.

Grading standard of soybean root rot: level 0: the plant grows normally; level 1: more than 1/2 of the leaves of the plants turn yellow; and 2, stage: more than 3/4 of plant leaves turn yellow and obviously senilism; and 3, level: more than 3/4 of plant leaves turn yellow and obviously senilism; and 4, stage 4: the yellow color of the plant leaves is more than 3/4.

Incidence A (%) = diseased plants/investigated plants × 100%

Relative control effect B (%) = (disease index in control zone-disease index in treatment zone)/disease index in control zone × 100%

TABLE 12 field experiment results of blending fluazinam, fludioxonil and metalaxyl-M for soybean root rot

Wherein: gem sperm suspension seed coating C (metalaxyl-M: fludioxonil 3.

As can be seen, the control effect of fludioxonil in the single agent is best 42.5%, fluazinam is 41.6%, and the control effect of metalaxyl-M is worst 12.6%. In the compound agent, the relative control effect of the seed dressing medicament Jingsong commonly used in production reaches 53.5%, the morbidity rate is reduced by nearly 30%, and the weight ratio of fluazinam, fludioxonil and metalaxyl-M is 5:5:2 when the components are mixed, the relative prevention effect can reach 76.3 percent, the morbidity is reduced by 46.2 percent, and the soybean root rot prevention and treatment effect is good.

Claims

1. The pesticide sterilization composition containing metalaxyl-M is characterized in that the active ingredients of the pesticide sterilization composition are fluazinam, fludioxonil and metalaxyl-M.

2. The agricultural chemical sterilizing composition containing metalaxyl-M according to claim 1, wherein the weight ratio of fluazinam, fludioxonil and metalaxyl-M is (1-12): (1-13): (1-8).

3. The use of the agricultural chemical sterilizing composition containing metalaxyl-M according to claim 1 or 2, which is used for preparing an agricultural chemical preparation;

the pesticide preparation is prepared from any one of dispersible oil suspending agent, seed treatment suspending agent, microcapsule suspending agent, microemulsion, granules, emulsion in water, wettable powder and water dispersible granules.

4. The use of the agricultural chemical sterilizing composition containing metalaxyl-M according to claim 3 is characterized in that the formulation of the agricultural chemical preparation is a seed treatment suspending agent;

the pesticide formulation further comprises: wetting dispersant, film forming agent, thickening agent, warning color, antifreezing agent, defoaming agent, preservative and water.

5. The application of the agricultural chemical sterilizing composition containing metalaxyl-M according to claim 3 is characterized in that the mass percentage concentration of the agricultural chemical sterilizing composition containing metalaxyl-M in an agricultural chemical preparation is 5-30%.

6. The use of the agricultural chemical composition containing metalaxyl-M as claimed in claim 5, wherein the wetting dispersant is at least one selected from fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkyl glycoside, fatty amine polyoxyethylene ether, lignosulfonate, alkylphenol resin polyoxyethylene ether sulfate, methyl naphthalene sulfonic acid formaldehyde condensate sulfate, aryl polyoxyethylene alkyl sulfate, trisiloxane polyoxyethylene ether, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, and castor oil polyoxyethylene ether.

7. The use of the agricultural chemical composition containing metalaxyl-M as claimed in claim 6, wherein the film forming agent is at least one selected from the group consisting of polyvinyl alcohol, chitosan, polyvinyl acetate, carboxymethyl cellulose, xanthan gum, acrylic resin, polyurethane copolymer resin, acrylate modified butadiene resin, polyurethane modified nitrocellulose, and attapulgite.

8. The use of the agricultural chemical composition containing metalaxyl-M as claimed in claim 3, wherein the thickener is at least one selected from the group consisting of starch, sodium starch phosphate, gum arabic, pectin, agar, gelatin, algin, carrageenan, dextrin, sodium carboxymethylcellulose, propylene glycol alginate, methyl cellulose, sodium alginate, casein, and polyvinylpyrrolidone.

9. The use of the agricultural chemical germicide composition containing metalaxyl-M as claimed in claim 3, characterized in that the antifreezing agent is at least one selected from isopropanol, ethylene glycol, propylene glycol butyl ether, ethylene glycol butyl ether acetate, formamide, calcium chloride, sodium acetate and urea.

10. The use of the agricultural chemical germicide composition containing metalaxyl-M according to claim 3, characterized in that the defoaming agent is selected from at least one of polyglycerol, lauric acid, isooctanol, stearic acid, epoxidized soybean oil, polyoxypropylene glycerol ether, silicone oil, polyglycol ether and polyether modified silicon.