CN115803453A - 使用经修饰的捕获引物捕获和扩增靶多核苷酸的组合物和方法 - Google Patents

使用经修饰的捕获引物捕获和扩增靶多核苷酸的组合物和方法 Download PDF

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CN115803453A
CN115803453A CN202180041929.7A CN202180041929A CN115803453A CN 115803453 A CN115803453 A CN 115803453A CN 202180041929 A CN202180041929 A CN 202180041929A CN 115803453 A CN115803453 A CN 115803453A
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capture
orthogonal
duplex
modified nucleic
primer
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Chinese (zh)
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H·布莱克
E·阿米霍
M·莱萨德-维格
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Illumina Inc
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Illumina Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
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  • Microbiology (AREA)
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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180041929.7A 2020-12-21 2021-12-20 使用经修饰的捕获引物捕获和扩增靶多核苷酸的组合物和方法 Pending CN115803453A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063128675P 2020-12-21 2020-12-21
US63/128,675 2020-12-21
PCT/US2021/064342 WO2022140254A1 (en) 2020-12-21 2021-12-20 Compositions and methods for capturing and amplifying target polynucleotides using modified capture primers

Publications (1)

Publication Number Publication Date
CN115803453A true CN115803453A (zh) 2023-03-14

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CN202180041929.7A Pending CN115803453A (zh) 2020-12-21 2021-12-20 使用经修饰的捕获引物捕获和扩增靶多核苷酸的组合物和方法

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US (1) US20240011080A1 (enExample)
EP (1) EP4263860A1 (enExample)
JP (1) JP7870260B2 (enExample)
KR (1) KR20230123872A (enExample)
CN (1) CN115803453A (enExample)
AU (1) AU2021409643A1 (enExample)
CA (1) CA3183729A1 (enExample)
IL (1) IL299521A (enExample)
MX (1) MX2022014813A (enExample)
TW (1) TW202233849A (enExample)
WO (1) WO2022140254A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026076125A1 (en) * 2024-10-01 2026-04-09 Illumina, Inc. Increasing spatial transcriptomics library yield by surface amplification of index-labeled cdna

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127569A1 (en) * 1997-05-02 2002-09-12 Weisburg William G. Two-step hybridization and capture of a polynucleotide
WO2013102090A1 (en) * 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Isothermal amplification systems and methods
US20140031240A1 (en) * 2012-07-03 2014-01-30 Foundation Medicine, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6884596B2 (en) * 2000-04-28 2005-04-26 The Regents Of The University Of California Screening and therapeutic methods for promoting wakefulness and sleep
AU2003288474A1 (en) * 2002-06-24 2004-03-19 Exiqon A/S Methods and systems for detection and isolation of a nucleotide sequence
JP2007222010A (ja) * 2006-02-21 2007-09-06 Sumitomo Bakelite Co Ltd 遺伝子の検出方法及び遺伝子検出用担体
EP1914317A1 (en) * 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127569A1 (en) * 1997-05-02 2002-09-12 Weisburg William G. Two-step hybridization and capture of a polynucleotide
WO2013102090A1 (en) * 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Isothermal amplification systems and methods
US20140031240A1 (en) * 2012-07-03 2014-01-30 Foundation Medicine, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

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MX2022014813A (es) 2023-03-07
US20240011080A1 (en) 2024-01-11
JP7870260B2 (ja) 2026-06-04
EP4263860A1 (en) 2023-10-25
IL299521A (en) 2023-02-01
KR20230123872A (ko) 2023-08-24
CA3183729A1 (en) 2022-06-30
WO2022140254A1 (en) 2022-06-30
AU2021409643A1 (en) 2023-01-05
JP2024500260A (ja) 2024-01-09
TW202233849A (zh) 2022-09-01

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