CN115777726A - Seed treatment composition containing paenibacillus polymyxa KN-03 and application thereof - Google Patents
Seed treatment composition containing paenibacillus polymyxa KN-03 and application thereof Download PDFInfo
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- CN115777726A CN115777726A CN202211079948.1A CN202211079948A CN115777726A CN 115777726 A CN115777726 A CN 115777726A CN 202211079948 A CN202211079948 A CN 202211079948A CN 115777726 A CN115777726 A CN 115777726A
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Abstract
The invention discloses a seed treatment composition containing paenibacillus polymyxa KN-03, which comprises the following effective components of paenibacillus polymyxa KN-03 and tebuconazole in a mass ratio of 100. The seed treatment composition overcomes the defects of low germination rate, low emergence rate, inhibited root growth and the like of wheat caused by treating seeds with tebuconazole with higher concentration, can promote the root growth of wheat seedlings and delay the generation of resistance of pathogenic bacteria, and is suitable for preventing and treating wheat sharp eyespot.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a seed treatment composition containing Paenibacillus polymyxa KN-03 and application thereof in preventing and treating crop diseases.
Background
The wheat sharp eyespot is a soil-borne fungal disease caused by the infection of rhizoctonia cerealis, and the sclerotium can overwinter in soil and can also overwinter on crop disease residues and weeds by hypha and sclerotium, so that the wheat sharp eyespot becomes a main initial infection source in the next year. The disease causes that wheat cannot spike or blighted grains of the spike are more, and the grain weight is reduced. In recent years, the country advocates returning straws to the field, which is beneficial to protecting the environment and increasing the organic matters of soil, but simultaneously causes the annual increase of sheath blight and 50% reduction of the yield of the land with serious disease. The prevention and control of wheat sharp eyespot become the focus of people's attention. The optimal period of soil-borne disease control is not easy to grasp in the conventional spraying, and once the optimal period of control is missed, irreparable loss can be caused. And seed treatment is the most economical and effective method for preventing and treating soil-borne diseases. Seed treatment typically includes dressing, soaking, coating, and the like. The seed treatment agent for preventing and treating the wheat sharp eyespot is few, and the conventional medicaments such as thiram, triadimefon, carbendazim, diniconazole and the like have generated resistance to different degrees, so that the prevention effect is obviously reduced. Tebuconazole is a high-efficiency, broad-spectrum and systemic triazole fungicide, is widely used on a seed treatment agent, has a good control effect on wheat sharp eyespot, but has some problems in the use process, such as the tebuconazole with higher concentration can inhibit the germination of wheat seeds, delay the emergence time, reduce the emergence rate, inhibit the growth of seedlings, inhibit the growth of root systems, increase the number of malformed plants, easily generate phytotoxicity under the condition of low-temperature stress, and possibly cause the generation of drug resistance along with the increase of the use frequency and the use amount.
Paenibacillus polymyxa KN-03 (paenibaci Illus polymyxa KN-03) is separated and screened from soil samples of Shandong tomato planting demonstration base by Wuhan Keno biotech GmbH, and is preserved in China center for type culture Collection at 3/13 of 2012 with the preservation number of CCTCC NO: M2012077. Paenibacillus polymyxa KN-03 belongs to a bacterial biological bactericide, is a gram-positive bacterium of endophytic spores, successfully colonizes to the rhizosphere, the body surface or the body of a plant, competes with pathogenic bacteria for nutrition around the plant, secretes antibacterial substances to inhibit the growth of the pathogenic bacteria, and induces a plant defense system to resist the invasion of the pathogenic bacteria, so that the purpose of biological control is achieved. The pesticide has certain control effect on wheat scab, strawberry bacterial wilt, watermelon fusarium wilt and other soil-borne diseases. The registered use method of the pesticide for preventing and treating soil-borne diseases in China mainly comprises root irrigation and seed soaking, and seed coating and seed dressing are not registered.
Based on the problems, the inventor conducts a large number of formulation screening tests, finds that the paenibacillus polymyxa KN-03 and tebuconazole are compounded to be used as seed coating or seed dressing, has obviously higher control effect on rhizoctonia cerealis than a control single agent, has high safety, germination rate and emergence rate, overcomes the defects of low wheat germination rate, low emergence rate and inhibited root system growth caused by treating seeds with a tebuconazole single agent with higher concentration, and shows obvious root growth promoting effect and yield increasing effect. In the existing seed treatment agents, the report that the paenibacillus polymyxa KN-03 and tebuconazole seed treatment composition is used for preventing and treating wheat sharp eyespot is not found.
Disclosure of Invention
The invention aims to provide a seed treatment composition containing paenibacillus polymyxa KN-03, which is beneficial to promoting the growth of wheat root systems, improving the germination rate, emergence rate and yield of wheat and improving the control effect on wheat sharp eyespot.
In order to achieve the purpose, the invention adopts the following technical scheme:
the seed treatment composition containing the paenibacillus polymyxa KN-03 comprises the following effective components in a mass ratio of 100.
Further, the seed treatment composition containing the paenibacillus polymyxa KN-03 comprises the following effective components in a mass ratio of 50 to 4, preferably 30 to 10.
The seed treatment composition containing the paenibacillus polymyxa KN-03 is characterized in that the paenibacillus polymyxa in the composition accounts for 1-30% of the total mass of the composition, and the tebuconazole accounts for 0.1-30% of the total mass of the composition.
The dosage form of the seed treatment composition is a suspension seed coating agent or a seed treatment dispersible powder, and the application method is seed coating or seed dressing.
Furthermore, the seed treatment composition is suitable for preventing and treating soil-borne diseases such as wheat sharp eyespot, wheat basal stem rot, potato black nevus, watermelon fusarium wilt and the like, and is particularly suitable for preventing and treating wheat sharp eyespot.
The seed treatment composition comprises effective components, auxiliary agents and fillers.
The active ingredients comprise paenibacillus polymyxa KN-03 and tebuconazole, and the auxiliary agent is one or a combination of more than two of a wetting agent, a dispersing agent, a film forming agent, a thickening agent, a defoaming agent, an antifreezing agent, a preservative and a warning color.
The filler is selected from one or a combination of more than two of white carbon black, kaolin, diatomite, bentonite, talcum powder, argil, montmorillonite, clay, deionized water and tap water.
The components are processed according to the conventional processing method of the pesticide seed treatment agent to prepare the suspension seed coating agent and the seed treatment dispersible powder.
The method of application of the seed treatment composition of the present invention can be used for, but not limited to, seed coating or dressing, and can also be applied to seed soaking and the like.
The application range of the seed treatment composition can be applied to, but is not limited to, a suspension seed coating agent and a seed treatment dispersible powder, and can also be applied to pesticide formulations such as wettable powder, a seed treatment suspending agent, a water dispersible granule, a seed treatment dry powder, a dry seed mixing agent, a wet seed mixing agent, a seed treatment microcapsule suspending agent and the like.
Compared with the prior art, the invention has the following beneficial effects:
(1) The seed treatment composition overcomes the defects of low wheat germination rate, low emergence rate and inhibited root growth caused by treating seeds with tebuconazole with higher concentration;
(2) The synergistic effect of the seed treatment composition for preventing and treating the wheat sharp eyespot is obvious;
(3) The seed treatment composition can obviously promote the root growth of wheat seedlings;
(4) The seed treatment composition has obvious yield increasing effect;
(5) In the seed treatment composition, the paenibacillus polymyxa KN-03 has a special action mechanism and is compounded with tebuconazole, so that the action sites are increased, and the risk of drug resistance of pathogenic bacteria of sheath blight to the tebuconazole is reduced;
(6) The seed treatment composition greatly reduces the using amount of chemical pesticides, and is safe and environment-friendly.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described below with reference to the following embodiments.
Examples of indoor bioassay test
Indoor bioassay test of Paenibacillus polymyxa KN-03 and tebuconazole compound pairing Rhizoctonia cerealis
Test subjects: rhizoctonia cerealis (Rhizoctonia cerealis).
The test method refers to agricultural industry standard of the people's republic of China, agricultural laboratory bioassay test criterion NY/T1156.2-2006, and adopts a hypha growth rate method. The paenibacillus polymyxa KN-03 mother drug and tebuconazole raw drug are respectively dissolved by distilled water and acetone, and then a single agent and each mixed agent are diluted into 5 different mass concentration gradients by 0.1 percent of Tween-80 aqueous solution. Clear water was set as a blank control and repeated 3 times per treatment. Cutting off the cultured pathogenic bacteria from the edges of bacterial colonies by using a puncher with the diameter of 5mm under the aseptic condition, inoculating the bacterial cakes onto culture medium flat plates with different medicament concentrations by using an inoculating needle, and culturing in a thermostat at 26 ℃ for 48 hours; checking colony diameter, calculating percentage of inhibition of hypha growth of each agent, and determining EC of each agent by linear regression analysis between the probability of inhibition and the logarithm of the series concentration 50 The activity of the test agent on pathogenic bacteria is evaluated by calculating the co-toxicity coefficient (CTC) of the mixture by the Sun Yunpei method. The co-toxicity coefficient (CTC) of the compound agent is more than or equal to 120, which shows a synergistic effect; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects.
TABLE 1 indoor toxicity test results of the combination of Paenibacillus polymyxa KN-03 and tebuconazole on Rhizoctonia cerealis
The indoor toxicity test result shows that: the paenibacillus polymyxa KN-03 and tebuconazole are mixed according to the mass ratio of 100 to 1, have better toxicity to rhizoctonia cerealis, and are expressed as synergistic effects. Wherein, the paenibacillus polymyxa KN-03 and the tebuconazole are compounded according to the proportion of 30-10.
Formulation examples of formulations
Dispersible powder for seed treatment
According to the formula requirements, uniformly premixing the effective components, wetting agent, dispersing agent, warning color and filler, pulverizing, uniformly mixing, controlling indexes, detecting at any time, discharging after qualification, and subpackaging to obtain the seed treatment dispersible powder.
Examples 1-3 below all follow the above preparation process.
Example 1:10.1% Paenibacillus polymyxa KN-03. Tebuconazole seed treatment dispersible powder
Example 2:20.4% Paenibacillus polymyxa KN-03. Tebuconazole seed treatment dispersible powder
Example 3:60% paenibacillus polymyxa KN-03 tebuconazole seed treatment dispersible powder
Suspension seed coating agent
According to the formula requirement, mixing the effective components, wetting agent, dispersing agent, film-forming agent, thickening agent, antifreezing agent, defoaming agent, preservative, warning color and deionized water, high-shear pre-dispersing, adding into a sanding kettle, and grinding until the particle size is less than 5 μm to obtain the suspended seed coating agent.
The following examples 4 to 6 were all made according to the above preparation process.
Example 4:4.2% Paenibacillus polymyxa KN-03 tebuconazole suspension seed coating agent
Example 5:6.2% paenibacillus polymyxa KN-03 tebuconazole suspension seed coating agent
Example 6:5% paenibacillus polymyxa KN-03 tebuconazole suspension seed coating agent
Example 7:1.1% paenibacillus polymyxa KN-03 tebuconazole suspension seed coating agent
Indoor germination percentage and emergence rate examples
In order to observe the influence of different compound seed treatment compositions on the germination rate and the emergence rate of wheat seeds, the germination rate and the emergence rate of the wheat seeds are measured according to the test standard GB/T3543.4-1995, and an earth culture method is adopted.
Table 2 results of the influence of different compounded seed treatment compositions on the germination rate and emergence rate of wheat
As can be seen from the table 2, after the wheat seeds are treated by the paenibacillus polymyxa KN-03 and the tebuconazole according to the compositions with different proportions, the germination rate and the emergence rate of the wheat are obviously improved compared with the single-dose comparison of the tebuconazole and are slightly higher than the comparison of clear water, and the germination rate and the emergence rate of the wheat seeds can be improved by compounding the paenibacillus polymyxa KN-03 and the tebuconazole according to a certain proportion, so that the influence of the higher-concentration tebuconazole treated seeds on the germination rate and the emergence rate of the wheat is overcome.
Examples of the effects of the field
The method for testing the pesticide effect in the field refers to GB/T17980.108-2004. In 2018, 10 months to 2019, 6 months, a field test of the preparation examples 1 to 7 for coating wheat seeds to prevent and control wheat sharp eyespot is carried out in xi Zhang village in Boai county of Henan province. The contrast agents are 100 hundred million CFU/gram of paenibacillus polymyxa KN-03 suspension seed coating agent, 6 percent of tebuconazole suspension seed coating agent and 15 percent of imidacloprid-thiram-diniconazole suspension seed coating agent. The experiment is totally provided with 9 treatments, each treatment is repeated 4 times, the total time is 36 cells, and the cell area is 20m 2 Random block permutation is adopted. The test is carried out in 2018, 10 and 8 days, the seeds are coated or mixed, the seeds are air-dried in a shade place for standby, the seeds are sowed in 10 months and 10 days, and other bactericides are not applied between the sowing and the investigation result.
Crop health survey: the wheat growth condition of each treatment area is investigated and recorded on 27 days in 11 months by investigating 5 points for each treatment area, 200 plants per point, and the indexes comprise root length, root number and the like.
And (3) drug effect investigation: and (3) investigating the occurrence condition of the sheath blight disease of each treatment area in 3 months, wherein the method comprises the steps of investigating 5 points and 200 strains per point for each treatment area, investigating the morbidity index of the sheath blight disease of each cell, and counting the control effect.
Survey of yield: the wheat yield of each treatment is investigated in 5 days in 6 months, 5 points are investigated in each treatment area, indexes such as the effective ear number per mu (1 square meter of the effective ear number per point), the ear grain number (30 ears are taken per point, the ear grain number per ear is counted), thousand grain weight and the like are investigated, and the yield per mu of each treatment is calculated.
TABLE 3 results of the effects of different compounded seed treatment compositions on the root system of wheat seedlings
As can be seen from Table 3, after the wheat seeds are treated by the paenibacillus polymyxa KN-03 and the tebuconazole according to the composition with different proportions, the number and the root length of the wheat seeds are obviously increased compared with the single dose control of the tebuconazole and the clear water control, and the paenibacillus polymyxa KN-03 and the tebuconazole are compounded according to a certain proportion, so that the inhibition effect of the tebuconazole with higher concentration on the wheat root systems is overcome, and the obvious root system growth promotion effect is shown.
TABLE 4 control Effect of different combinations of seed treatment compositions on wheat sheath blight
As can be seen from Table 4, after the paenibacillus polymyxa KN-03 and the tebuconazole are used for treating the wheat seeds by the composition in different proportions, the control effect on the wheat sharp eyespot is over 86 percent and is obviously higher than that of a single agent control and a conventional agent control, and the paenibacillus polymyxa KN-03 and the tebuconazole are matched with each other in a certain proportion to have a good synergistic effect on the wheat sharp eyespot.
TABLE 5 Effect of different Complex seed coating compositions on wheat yield
As can be seen from Table 5, the treatment of wheat seeds with the compositions of the present invention in different proportions by Paenibacillus polymyxa KN-03 and tebuconazole has a significant effect on the wheat yield per mu. The wheat yield per mu treated by the compositions with different proportions is obviously higher than that of a single-dose control, a conventional medicament control and a clear water control, the wheat yield per mu increase ratio is obviously higher than that of the single-dose control and the conventional medicament control, and is up to 39 percent, so that the wheat yield per mu can be obviously increased by compounding the paenibacillus polymyxa KN-03 and tebuconazole in a certain proportion.
As can be seen from tables 2, 3, 4 and 5, the seed treatment agent compounded by the paenibacillus polymyxa KN-03 and the tebuconazole is used for preventing and treating the wheat sharp eyespot, so that the dosage of chemical pesticides can be reduced, and the environmental pollution can be reduced.
The present invention is described above by way of example with reference to the embodiments, and it is obvious that the implementation of the present invention is not limited by the above embodiments, and it is within the scope of the present invention to adopt various modifications of the method concept and technical scheme of the present invention, or to directly apply the concept and technical scheme of the present invention to other occasions without modification.
Claims (6)
1. A seed treatment composition containing Paenibacillus polymyxa KN-03 is characterized in that the effective components of the seed treatment composition are the Paenibacillus polymyxa KN-03 and tebuconazole, and the mass ratio of the two is 100-1.
2. The seed treatment composition according to claim 1, wherein the mass ratio of the effective components of paenibacillus polymyxa KN-03 and tebuconazole is 50.
3. The seed treatment composition according to claim 2, wherein the mass ratio of the effective components of paenibacillus polymyxa KN-03 and tebuconazole is 30-10.
4. The seed treatment composition according to claim 1, wherein the effective component paenibacillus polymyxa KN-03 accounts for 1-30% of the total mass of the composition, and the tebuconazole accounts for 0.1-30% of the total mass of the composition.
5. The seed treatment composition of claim 4, wherein the dosage form is a suspended seed coating or a seed treatment dispersible powder.
6. Use of the seed treatment composition according to claims 1 to 5 for controlling sheath blight of wheat.
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