CN115777581B - Contact type high-flux egg embryo activity detection equipment - Google Patents
Contact type high-flux egg embryo activity detection equipment Download PDFInfo
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- CN115777581B CN115777581B CN202310043268.2A CN202310043268A CN115777581B CN 115777581 B CN115777581 B CN 115777581B CN 202310043268 A CN202310043268 A CN 202310043268A CN 115777581 B CN115777581 B CN 115777581B
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Abstract
The invention provides a contact type high-flux egg embryo activity detection device, which comprises: the invention relates to a closed metal shading camera bellows used for collecting egg embryo signals, which comprises a guide rail used for sending egg trays into and out of the shading camera bellows, an upper computer used for receiving, processing and sending signals and a display, wherein the camera bellows comprises an egg embryo signal collecting lower computer, a motor, a bracket and the guide rail, the lower computer used for carrying out wireless transmission comprises an electric control system and a router used for carrying out wireless transmission, and the egg embryo signal collecting lower computer comprises 150 parallel collecting channels.
Description
Technical Field
The invention relates to the field of spectrum acquisition, in particular to contact type high-flux egg embryo activity detection equipment.
Background
One egg has a plurality of detection requirements, the most important detection requirement is the activity detection of the egg embryo, because the detection effect plays a decisive role in the productivity of the poultry breeding industry and the vaccine preparation industry, the effect is more prominent in the whole vaccine preparation process, and most influenza vaccines are produced by taking live egg embryo as a carrier, so the activity detection is required for the egg embryo of 10 days before virus inoculation, the activity detection is still required every day after virus inoculation, dead embryo is removed in time, and unnecessary cross contamination and waste are reduced. According to investigation, in the current egg embryo activity detection methods, the pulse wave detection method is superior to other detection methods, but the existing detection equipment has a practical application defect, and the defect is that: as the embryo disc of an egg grows mature, the acquisition of pulse waves can be affected, so that the accuracy of the pulse wave detection method is seriously reduced. The invention provides a contact 45-degree correlation acquisition scheme, which effectively solves the problem of influence on signal acquisition caused by placenta development, improves accuracy and meets actual industrial requirements.
In the development process of fertilized egg embryo, due to the formation of placenta, the incident light of the one hundred eighty degree vertical transmission type pulse wave acquisition device can be shielded by the placenta, so that the acquired pulse wave signal effect is poor, and even the pulse wave signal cannot be acquired. Experiments prove that the device has good collection effect on 9-12-day-old egg embryos, the collection effect on high-day-old egg embryos is poor, and the classification effect is obviously reduced. According to lambert beer law, the longer the light path is, the stronger the absorbance is, and the information carried by the signal is more abundant, so that the contact 45-degree correlation acquisition device is designed, and the defect in the prior art is overcome.
Disclosure of Invention
The invention aims to overcome the defects of the technology, and provides contact type high-flux egg embryo activity detection equipment which is used for serving the vaccine preparation industry and the poultry farming industry, so that the waste in the vaccine preparation process is greatly reduced, the vaccine quality is ensured, and the productivity of the poultry farming industry is improved.
In order to achieve the above purpose, the present invention provides the following technical solutions: a contact high throughput embryo activity assay device comprising: a metal shading camera bellows, an egg tray conveying component for conveying egg trays into and out of the metal shading camera bellows, an upper computer for receiving, processing and sending signals and a display,
the display is arranged on the metal shading dark box; the egg tray conveying assembly penetrates through the metal shading dark box and divides the metal shading dark box into an upper layer box and a lower layer box; the left and right sides of the metal shading dark box are respectively provided with a left electric door and a right electric door, and when the signal acquisition is carried out on the egg embryo, the left electric door and the right electric door are closed; the egg tray enters the metal shading dark box from the left side electric door and leaves the metal shading dark box from the right side electric door;
the upper layer box of the metal shading dark box is internally provided with a lower computer, a lifting motor, a horizontal bracket and a vertical guide rail, wherein the lower computer is connected with the vertical guide rail through the horizontal bracket, the lifting motor is connected with the vertical guide rail, the vertical guide rail comprises a screw rod and a guide rail which are mutually parallel, the horizontal bracket is in threaded connection with the screw rod and is in sliding connection with the guide rail, the lifting motor drives the screw rod to rotate so as to drive the horizontal bracket to move upwards or downwards,
the lower computer comprises a plurality of contact type egg embryo signal acquisition devices, for example, 150 contact type egg embryo signal acquisition devices, all the contact type egg embryo signal acquisition devices acquire signals of egg embryos at the same time, namely, 150 egg embryos are acquired at the same time, the main body of the contact type egg embryo signal acquisition device is a rubber sleeve, a patch type LED and a patch type photosensitive sensor are horizontally and relatively embedded at the edge of the bottom end of the rubber sleeve, the patch type LED and the patch type photosensitive sensor are respectively connected with a lead wire, and the lead wire extends upwards from the inside of the rubber sleeve and penetrates out from an opening at the top of the rubber sleeve; after the contact type egg embryo signal acquisition device is attached to an egg body, the contact type egg embryo signal acquisition device irradiates from the direction of 45 degrees of the horizontal included angle of the egg embryo through a patch type LED, and receives optical signals at the position of 135 degrees of the horizontal included angle of the opposite side through the patch type photosensitive sensor to acquire pulse wave and blood oxygen signals of the egg embryo;
the lower layer box of the metal shading dark box is internally provided with an electric control system, an upper computer and a router for wireless transmission, the upper computer is respectively connected with the electric control system, the lower computer, a display and the router, and the electric control system is respectively connected with the lifting motor, the egg tray conveying assembly, the left side electric door and the right side electric door.
Further, the rubber sleeve comprises an outer rubber layer and an inner rubber layer which are connected, and the lead is positioned between the outer rubber layer and the inner rubber layer.
Further, the patch type LED integrates two light sources, namely a 600nm red light source and a 900nm near infrared light source.
Further, the egg tray conveying assembly comprises an egg tray guide rail, an egg tray limiter, a horizontal conveying motor and a motor track, wherein the motor track parallel to the egg tray guide rail is arranged beside the egg tray guide rail, the horizontal conveying motor is connected with the motor track, a sliding block is connected with the motor track, the motor track comprises a screw rod and a guide rail which are mutually parallel, the sliding block is in threaded connection with the screw rod and is in sliding connection with the guide rail, the horizontal conveying motor drives the screw rod to rotate so as to drive the horizontal bracket sliding block to move leftwards or rightwards, a telescopic cylinder is fixed on the sliding block and is connected with the egg tray limiter, and the egg tray limiter is provided with two groups of clamping jaws, each group of clamping jaws is used for clamping an egg tray; the metal shading camera bellows is internally provided with a proximity sensor, and the proximity sensor is positioned beside the egg tray guide rail and used for positioning the egg tray.
Further, the egg tray guide rail penetrates through the whole metal shading dark box from left to right, the initial position of the first egg tray is arranged at the left side of the egg tray guide rail, the telescopic cylinder drives the clamping jaw of the egg tray limiter to extend out, and the egg tray limiter is used for fixing the first egg tray;
the method comprises the steps that a box entering instruction is sent by an upper computer, a horizontal transmission motor drives a sliding block to move rightwards on a motor track, a first egg tray moves to the inside of a metal shading camera bellows along an egg tray guide rail, after a proximity sensor detects that the first egg tray approaches to a designated position, the horizontal transmission motor stops working, and the first egg tray stops at a signal acquisition position under a lower computer; the telescopic cylinder drives the clamping jaw of the egg tray limiter to retract, and the horizontal transmission motor drives the sliding block to drive the egg tray limiter to return to the left initial position; a second egg tray is placed at the left side of the egg tray guide rail, the telescopic cylinder drives the clamping jaw to extend out, and the first egg tray and the second egg tray are respectively fixed in the egg tray limiter through two groups of clamping jaws;
the lifting motor controls the lower computer to descend until the lower ports of the rubber sleeves of all the contact egg embryo signal acquisition devices are attached to all egg bodies on the first egg tray, the lifting motor stops working, and the lower computer hovers; the lower computer collects signals of the egg embryo, and after the collection is finished, the lower computer is driven by the lifting motor to rise to an initial position;
the horizontal transmission motor drives the sliding block to move rightwards on the motor track, drives the first egg tray and the second egg tray fixed by the egg tray limiter to move rightwards, and the first egg tray moves to the right side of the metal shading dark box, and the second egg tray moves into the metal shading dark box.
Further, the upper layer box of the metal shading dark box is provided with a double-opening sliding door at the upper layer of the dark box, and is used for debugging, maintenance and internal cleaning at the later stage of equipment; the lower layer box of the metal shading dark box is provided with a box body lower layer split sliding door for debugging and maintaining hardware.
Further, the display is used for operating the device through software of the contact type high-flux egg embryo activity detection device, and checking the collection condition of egg embryo signals and the activity information of egg embryos.
Further, the software of the contact type high-flux egg embryo activity detection device displays the working condition, the wireless connection condition, the egg embryo signal acquisition condition and the activity information of all 150 detected egg embryos to a user through a visual interface, and the operation of equipment on-off, egg tray in-box, egg tray out-box, signal acquisition, activity discrimination, data storage and data viewing is executed through the software.
Further, the visual interface of the contact type high-throughput egg embryo activity detection equipment software can display egg embryo activity information including a score table, blood oxygen values, PPG original waveform images and PPG filtered waveform images of all 150 detected egg embryos, and dead and alive classification images of all 150 egg embryos are plane images of egg flats after the same proportion is reduced, live egg embryo images display yellow, and dead egg embryo images display red.
Further, the scoring table of the egg embryo is that PPG signals of all 150 egg embryos are respectively made into sliding PSDs and then are used as input data sets, the sliding PSDs are input into an egg embryo activity algorithm based on CNN, the activity score of each egg embryo is obtained through the algorithm, the score value is between 0 and 1, the higher the number is, the stronger the activity of the egg embryo is represented, the lower the number is, the weaker the activity of the egg embryo is represented, the dead and alive classification is carried out on all egg embryos according to the score value by utilizing a set judging threshold, and the judging threshold can be reset in the software according to actual conditions.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims thereof as well as the appended drawings.
The technical scheme of the invention is further described in detail through the drawings and the embodiments.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention.
FIG. 1 is a front view of a contact high throughput embryo activity detection device of the present invention.
Fig. 2 is a front view of a contact type signal acquisition device according to the present invention.
Fig. 3 is a top view of a tray moving track according to the present invention.
In the figure: the device comprises a lifting motor, a vertical guide rail, a left side electric door 3, an egg tray limiter 4, a horizontal transmission motor 5, an egg tray guide rail 6, an upper computer 7, a metal shading camera bellows 8, a display 9, a horizontal support 10, an upper side split sliding door of the camera bellows 11, a lower computer 12, a contact egg embryo signal acquisition device 13, a right side electric door 14, a proximity sensor 15, a lower side split sliding door of the box 16, a router 17, an electric control system 18, a surface mounted LED19, a top opening 20, an outer rubber layer of a rubber sleeve 21, an inner rubber layer of the rubber sleeve 22, a lead wire 23, a surface mounted photosensitive sensor 24, a telescopic cylinder 25, a sliding block 26, a motor track 27 and a clamping jaw 28.
Detailed Description
Reference will now be made in detail to exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numbers in different drawings refer to the same or similar elements, unless otherwise indicated. The implementations described in the following exemplary examples are not representative of all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with some aspects of the present disclosure as detailed in the accompanying claims.
The invention aims to provide contact type high-flux egg embryo activity detection equipment, which solves the technical defects of the existing detection equipment, improves the accuracy and the application range of the activity detection equipment, is used for the vaccine preparation industry and the poultry farming industry, greatly reduces the waste in the vaccine preparation process, ensures the vaccine quality and improves the productivity of the poultry farming industry.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
A contact type high-throughput egg embryo activity detection device, as shown in fig. 1-3, comprising: the egg embryo signal collection time airtight metal shading camera bellows 8 is used for sending egg flats into and out of an egg flat conveying assembly of the metal shading camera bellows 8, an upper computer 7 used for receiving, processing and sending signals and a display 9.
The display 9 is arranged on the metal shading dark box 8; the egg tray conveying assembly penetrates through the metal shading dark box 8, and the metal shading dark box 8 is divided into an upper layer box and a lower layer box. The design mode of vertical separation makes space utilization more reasonable, has reduced the noise interference that different functional module produced to be convenient for the clearance of later stage equipment, adjustment, maintenance. The left and right sides of the metal shading dark box 8 are respectively provided with a left electric door 3 and a right electric door 14, and when the signal acquisition is carried out on the egg embryo, the left electric door 3 and the right electric door 14 are closed; the egg tray enters the metal shading dark box 8 from the left side electric door 3 and leaves the metal shading dark box 8 from the right side electric door 14. When the egg tray enters the interior, the electric door is closed, the signal acquisition is completed, and the electric door is opened, so that the design ensures the required full dark environment condition during acquisition.
The upper layer of the metal shading camera bellows 8 is internally provided with a lower computer 12, a lifting motor 1, a horizontal support 10 and a vertical guide rail 2, wherein the lower computer 12 is connected with the vertical guide rail 2 through the horizontal support 10, the lifting motor 1 is connected with the vertical guide rail 2, the vertical guide rail 2 comprises a screw rod and a guide rail which are parallel to each other, the horizontal support 10 is in threaded connection with the screw rod and is in sliding connection with the guide rail, and the lifting motor 1 drives the screw rod to rotate so as to drive the horizontal support 10 to move upwards or downwards.
The lower computer 12 comprises 150 contact type egg embryo signal acquisition devices 13, all 150 contact type egg embryo signal acquisition devices 13 acquire signals of egg embryos at the same time, and each contact type egg embryo signal acquisition device 13 acquires signals of one egg embryo, namely, simultaneously acquires signals of 150 egg embryos. The main body of the contact type egg embryo signal acquisition device 13 is a rubber sleeve, a patch type LED19 and a patch type photosensitive sensor 24 are horizontally and relatively embedded at the edge of the bottom end of the rubber sleeve, the patch type LED19 and the patch type photosensitive sensor 24 are respectively connected with a lead 23, and the lead 23 extends upwards from the inside of the rubber sleeve and penetrates out from an opening 20 at the top of the rubber sleeve. The rubber sleeve comprises an outer rubber sleeve layer 21 and an inner rubber sleeve layer 22 which are connected, and the lead 23 is positioned between the outer rubber sleeve layer 21 and the inner rubber sleeve layer 22. The patch LED19 integrates two light sources, one 600nm red and one 900nm near infrared. After the contact type egg embryo signal acquisition device 13 is attached to an egg body, the contact type egg embryo signal acquisition device irradiates from the direction of 45 degrees of the horizontal included angle of the egg embryo through the surface mounted LED19, receives optical signals at the position of 135 degrees of the horizontal included angle of the opposite side through the surface mounted type photosensitive sensor 24, and acquires pulse wave and blood oxygen signals of the egg embryo. The contact type egg embryo signal acquisition device 13 not only avoids the influence of placenta on transmitted light, effectively improves the AC value of pulse wave signals, but also avoids the light saturation condition caused by overlarge light intensity compared with the same side correlation, so that the acquisition effect is improved, the problem of poor activity effect of detecting the day old egg embryo by the existing activity detection equipment is solved, meanwhile, signals with good quality are obtained, the data length required by subsequent modeling can be reduced, and real high-flux detection is realized.
The lower layer box of the metal shading dark box 8 is internally provided with an electric control system 18, an upper computer 7 and a router 17 for wireless transmission, the upper computer 7 is respectively connected with the electric control system 18, the lower computer 12, the display 9 and the router 17, and the electric control system 18 is respectively connected with the lifting motor 1, the egg tray conveying assembly, the left electric door 3 and the right electric door 14. In order to minimize the limitation of the connection of the system, the device is provided with a wireless router as a medium for wireless transmission of data, and the built-in router has the advantage of avoiding the problem of poor wireless signals caused by the metal material of the shading camera bellows.
The egg tray conveying assembly comprises an egg tray guide rail 6, an egg tray limiter 4, a horizontal conveying motor 5 and a motor track 27, wherein the motor track 27 parallel to the egg tray guide rail 6 is arranged beside the egg tray guide rail 6, the horizontal conveying motor 5 is connected with the motor track 27, a sliding block 26 is connected with the motor track 27, the motor track 27 comprises a screw rod and a guide rail which are parallel to each other, the sliding block 26 is in threaded connection with the screw rod and is in sliding connection with the guide rail, the horizontal conveying motor 5 drives the screw rod to rotate so as to drive the horizontal bracket sliding block 26 to move leftwards or rightwards, a telescopic cylinder 25 is fixed on the sliding block 26, the telescopic cylinder 25 is connected with the egg tray limiter 4, and two groups of clamping jaws 28 are arranged on the egg tray limiter 4, and each group of clamping jaws 28 is used for clamping an egg tray; a proximity sensor 15 is arranged in the metal shading dark box 8, and the proximity sensor 15 is positioned beside the egg tray guide rail 6 and used for positioning the egg tray. The design mode of the guide rail for conveying the egg blanks ensures the high efficiency characteristic of the equipment when the equipment needs to collect in batches, effectively ensures the full dark environment during collection in the camera bellows, reduces the interference of external light on signal collection, and improves the overall stability of the equipment.
The egg tray guide rail 6 penetrates through the whole metal shading dark box 8 from left to right, the initial position of the first egg tray is at the left side of the egg tray guide rail 6, the telescopic cylinder 25 drives the clamping jaw 28 of the egg tray limiter 4 to extend out, and the egg tray limiter 4 fixes the first egg tray;
the upper computer 7 sends a box feeding instruction, the horizontal transmission motor 5 drives the sliding block 26 to move rightwards on the motor track 27, the first egg tray moves to the inside of the metal shading dark box 8 along the egg tray guide rail 6, and after the proximity sensor 15 detects that the first egg tray approaches to a designated position, the horizontal transmission motor 5 stops working, and the first egg tray stops at a signal acquisition position under the lower computer 12; the telescopic cylinder 25 drives the clamping jaw 28 of the egg tray limiter 4 to retract, and the horizontal transmission motor 5 drives the sliding block 26 to drive the egg tray limiter 4 to return to the left initial position; a second egg tray is placed at the left side of the egg tray guide rail 6, the telescopic cylinder 25 drives the clamping jaw 28 to extend, and the first egg tray and the second egg tray are respectively fixed in the egg tray limiter 4 through the two groups of clamping jaws 28;
the upper computer 7 sends a detection instruction, the lifting motor 1 controls the lower computer 12 to descend until the lower ports of the rubber sleeves of all the contact egg embryo signal acquisition devices 13 are attached to all egg bodies on the first egg flat, the lifting motor 1 stops working, and the lower computer 12 hovers; the lower computer 12 collects signals of the egg embryo, and after the collection is finished, the lifting motor 1 drives the lower computer 12 to ascend to an initial position;
the horizontal transmission motor 5 drives the sliding block 26 to move rightwards on the motor track 27, drives the first egg tray and the second egg tray fixed by the egg tray limiter 4 to move rightwards, the first egg tray moves to the right side of the metal shading dark box 8, the second egg tray moves into the metal shading dark box 8, and the lower computer 12 collects signals at a position right below.
The upper layer box of the metal shading dark box 8 is provided with a double-opening sliding door 11 at the upper layer of the dark box, and is used for debugging, maintenance and internal cleaning at the later stage of equipment; the lower layer box of the metal shading dark box 8 is provided with a box body lower layer split sliding door 16 for debugging and maintaining hardware.
The display 9 is hung outside the upper layer box of the metal shading dark box 8 and is positioned above the side-by-side sliding door 11 on the upper layer of the dark box. The display 9 adopts a touch screen and is used for operating the equipment through software of the contact type high-flux egg embryo activity detection equipment, and meanwhile, the acquisition condition of egg embryo signals and the activity information of egg embryos are checked.
The software of the contact type high-flux egg embryo activity detection device displays the working condition, the wireless connection condition, the egg embryo signal acquisition condition and the activity information of all 150 detected egg embryos to a user through a visual interface, and the operation of equipment on-off, egg tray in-box, egg tray out-box, signal acquisition, activity judgment, data storage and data viewing is executed through the software.
The visual interface of the contact type high-throughput egg embryo activity detection equipment software can display egg embryo activity information comprising a score table, blood oxygen values, PPG original waveform images and PPG filtered waveform images of all 150 detected egg embryos, wherein the dead and alive classifying images of all 150 egg embryos are plane images of egg flats which are reduced in an equal proportion, live egg embryo images display yellow, and dead egg embryo images display red.
The PPG is a photoelectric volume pulse wave detection method, wherein the light absorption quantity is pulsed along with the pulse of arterial blood, the PPG is a noninvasive means for detecting the volume change of blood in egg embryo tissues, a light source is injected into the surface of the egg embryo from the 45-degree position of the air chamber end of the egg, the incident light is injected into the egg embryo tissues through the eggshells, and finally the incident light is injected out from the horizontally symmetrical 45-degree position of the egg through the absorption and scattering actions of the eggshells and the tissues; the light intensity information of the egg embryo is obtained by adopting a reflection type method, the light intensity transmitted from the egg embryo changes along with the contraction and the relaxation of blood vessels in the egg embryo, the signal of the light intensity change is converted into an electric signal by a photoelectric sensor, the change of volume pulse blood flow is obtained, the egg embryo is detected by utilizing a photoelectric volume pulse method, the embryo is thoroughly detected, and the obtained egg embryo activity information is rich.
The filtering method comprises the steps of band-pass Butterworth filter filtering, wavelet decomposition and reconstruction.
The scoring table of the egg embryos is that PPG signals of all 150 egg embryos are respectively made into sliding PSDs and then are used as input data sets to be input into an egg embryo activity algorithm based on CNN, the activity score of each egg embryo is obtained through the algorithm, the score value is between 0 and 1, the higher the number is, the stronger the activity of the egg embryo is represented, the lower the number is, the weaker the activity of the egg embryo is represented, the set judging threshold value is utilized to classify the dead and alive of all egg embryos according to the score value, and the judging threshold value can be reset in the software according to actual conditions.
The sliding PSD is sliding power spectral density, the data is enhanced by the method and used as the input of an activity algorithm, the characteristics are obvious in the frequency domain according to the egg embryo signals, and the characteristics are not changed with the time; the operation method comprises the following steps: for a standard periodic signal, a peak value can appear after fast Fourier transform, and the peak position on a spectrum image basically does not change along with the time; because of the randomness of noise, the position of peak value will fluctuate in the short time measurement process, the method of sliding power spectrum is shown in the figure, for a period of periodic signal, the first window is 1 st point to 6 th point, FFT is carried out on the period of periodic signal to form FFT-1, the second window is 2 nd point to 7 th point, FFT is carried out on the period of periodic signal to form FFT-2, and so on, the last window is 94 th point to 99 th point, FFT is carried out on the period of periodic signal to form FFT-94, and signals after FFT are combined to form sliding PSD.
The CNN is a convolutional neural network, is widely applied to the fields of computer vision and natural language processing, has the capability of adaptively extracting characteristics, can efficiently extract the characteristics of data, does not need to carry out excessive pre-processing on the data, and can greatly reduce the number of weights and reduce the complexity of a model compared with other prediction models by a special weight sharing mechanism; CNN passes through a plurality of hidden layers from an input layer to an output layer, so that complex information can be solved, complex function approximation is realized, and the classification accuracy is improved.
It will be appreciated by those skilled in the art that the first and second aspects of the present invention refer to different phases of application.
Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure disclosed herein. This application is intended to cover any adaptations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
It is to be understood that the present disclosure is not limited to the precise arrangements and instrumentalities shown in the drawings, and that various modifications and changes may be effected without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.
Claims (8)
1. A contact high throughput embryo activity assay device comprising: a metal shading camera bellows (8) which is used for sending egg trays into and out of an egg tray conveying assembly of the metal shading camera bellows (8), an upper computer (7) which is used for receiving, processing and sending signals and a display (9),
the display (9) is arranged on the metal shading dark box (8); the egg tray conveying assembly penetrates through the metal shading dark box (8) and divides the metal shading dark box (8) into an upper layer box and a lower layer box; the left and right sides of the metal shading dark box (8) are respectively provided with a left electric door (3) and a right electric door (14), and when the signal acquisition is carried out on the egg embryo, the left electric door (3) and the right electric door (14) are both closed;
a lower computer (12), a lifting motor (1), a horizontal bracket (10) and a vertical guide rail (2) for egg embryo signal acquisition are arranged in the upper layer box of the metal shading dark box (8), the lower computer (12) is connected with the vertical guide rail (2) through the horizontal bracket (10), the lifting motor (1) is connected with the vertical guide rail (2),
the lower computer (12) comprises a plurality of contact type egg embryo signal acquisition devices (13), the contact type egg embryo signal acquisition devices (13) are used for simultaneously acquiring signals of egg embryos, the main body of each contact type egg embryo signal acquisition device (13) is a rubber sleeve, a patch type LED (19) and a patch type photosensitive sensor (24) are horizontally and oppositely embedded at the edge of the bottom end of each rubber sleeve, the patch type LED (19) and the patch type photosensitive sensor (24) are respectively connected with a lead wire (23), and the lead wires (23) extend upwards from the inside of the rubber sleeve and penetrate out from an opening (20) at the top of the rubber sleeve; after the contact type egg embryo signal acquisition device (13) is attached to an egg body, the contact type egg embryo signal acquisition device irradiates from the direction of 45 degrees of the horizontal included angle of the egg embryo through a patch type LED (19), receives optical signals from the patch type photosensitive sensor (24) at the position of 135 degrees of the horizontal included angle of the opposite side, and acquires pulse wave and blood oxygen signals of the egg embryo;
an electric control system (18), an upper computer (7) and a router (17) for wireless transmission are arranged in a lower layer box of the metal shading dark box (8), the upper computer (7) is respectively connected with the electric control system (18), the lower computer (12), a display (9) and the router (17), and the electric control system (18) is respectively connected with the lifting motor (1), the egg tray conveying assembly, the left electric door (3) and the right electric door (14);
the egg tray conveying assembly comprises an egg tray guide rail (6), an egg tray limiter (4), a horizontal conveying motor (5) and a motor track (27), wherein the motor track (27) parallel to the egg tray guide rail (6) is arranged beside the egg tray guide rail, the horizontal conveying motor (5) is connected with the motor track (27), a sliding block (26) is connected with the motor track (27), a telescopic cylinder (25) is fixed on the sliding block (26), the telescopic cylinder (25) is connected with the egg tray limiter (4), the egg tray limiter (4) is provided with two groups of clamping jaws (28), and each group of clamping jaws (28) is used for clamping one egg tray; a proximity sensor (15) is arranged in the metal shading camera bellows (8), and the proximity sensor (15) is positioned beside the egg tray guide rail (6) and used for positioning the egg tray;
the egg tray guide rail (6) penetrates through the whole metal shading dark box (8) from left to right, the initial position of the first egg tray is arranged at the left side of the egg tray guide rail (6), the telescopic cylinder (25) drives the clamping jaw (28) of the egg tray limiter (4) to extend out, and the egg tray limiter (4) is used for fixing the first egg tray;
the horizontal transmission motor (5) drives the sliding block (26) to move rightwards on the motor track (27), the first egg tray moves to the inside of the metal shading dark box (8) along the egg tray guide rail (6), after the proximity sensor (15) detects that the first egg tray approaches to a designated position, the horizontal transmission motor (5) stops working, the telescopic cylinder (25) drives the clamping jaw (28) of the egg tray limiter (4) to retract, and the horizontal transmission motor (5) drives the sliding block (26) to drive the egg tray limiter (4) to return to the left initial position; a second egg tray is placed at the left side of the egg tray guide rail (6), the telescopic cylinder (25) drives the clamping jaw (28) to extend, and the first egg tray and the second egg tray are respectively fixed in the egg tray limiter (4) through the two groups of clamping jaws (28);
the lifting motor (1) controls the lower computer (12) to descend until the lower ports of the rubber sleeves of all the contact egg embryo signal acquisition devices (13) are attached to all egg bodies on the first egg tray, the lifting motor (1) stops working, and the lower computer (12) hovers; the lower computer (12) collects signals of the egg embryo, and after the collection is finished, the lifting motor (1) drives the lower computer (12) to ascend to an initial position;
the horizontal transmission motor (5) drives the sliding block (26) to move rightwards on the motor track (27) to drive the first egg tray and the second egg tray fixed by the egg tray limiter (4) to move rightwards, the first egg tray moves to the right side of the metal shading dark box (8), and the second egg tray moves into the metal shading dark box (8).
2. The contact high-throughput egg embryo activity detection device as claimed in claim 1, wherein said rubber sleeve comprises a rubber sleeve outer layer rubber (21) and a rubber sleeve inner layer rubber (22) connected, said lead wire (23) being located between said rubber sleeve outer layer rubber (21) and said rubber sleeve inner layer rubber (22).
3. A contact high throughput egg embryo activity detection device as claimed in claim 1, wherein said patch LED (19) incorporates two light sources, a 600nm red light source and a 900nm near infrared light source.
4. The contact type high-throughput egg embryo activity detection device as claimed in claim 1, wherein an upper layer box of the metal shading dark box (8) is provided with a dark box upper layer split sliding door (11); the lower layer box of the metal shading dark box (8) is provided with a box body lower layer split sliding door (16).
5. A contact high throughput embryo activity detection device as claimed in claim 1, in which said display (9) is adapted to operate the device by software of the contact high throughput embryo activity detection device whilst looking at the embryo signal acquisition and embryo activity information.
6. The contact type high-throughput egg embryo activity detection device as claimed in claim 5, wherein software of the contact type high-throughput egg embryo activity detection device displays the working condition, wireless connection condition, egg embryo signal acquisition condition and activity information of all detected egg embryos to a user through a visual interface, and the operations of device startup and shutdown, egg tray in-box, egg tray out-box, signal acquisition, activity discrimination, data storage and data viewing are executed through the software.
7. The touch high-throughput embryo activity assay device of claim 6, wherein the embryo activity information displayable on the visual interface of the touch high-throughput embryo activity assay device software comprises a score table, a blood oxygen value, a PPG raw waveform map, a PPG filtered waveform map of all detected embryos, and a dead-alive classification map of all embryos, wherein the dead-alive classification map is an isometric scaled-down plan view of an egg flat, the live embryo image shows yellow, and the dead embryo image shows red.
8. The contact type high-throughput embryo activity detection device according to claim 7, wherein the scoring table of the embryo is that PPG signals of all the embryos are respectively subjected to sliding PSD and then are input into a CNN-based embryo activity algorithm, the activity score of each embryo is obtained through the algorithm, the score value is between 0 and 1, the higher the number is, the stronger the embryo activity is represented, the lower the number is, the embryo activity is represented, the death and the activity of all the embryos are classified according to the score value by using a set judgment threshold, and the judgment threshold can be reset in the software according to practical conditions.
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| JP2001037367A (en) * | 1999-07-27 | 2001-02-13 | Kyowa Kikai Kk | Automatic selection system for egg and detection system for defective egg |
| US6850316B2 (en) * | 2002-06-10 | 2005-02-01 | Embrex, Inc. | Methods and apparatus for harvesting vaccine from eggs |
| CN103105498B (en) * | 2011-11-09 | 2014-07-16 | 青岛强星设备科技有限公司 | Full-automatic chick embryo inspection machine |
| CN102687684B (en) * | 2012-01-12 | 2015-04-15 | 河南科技大学 | Photographing platform for sorting eggs |
| CN102835330B (en) * | 2012-09-04 | 2015-04-29 | 青岛兴仪电子设备有限责任公司 | Automatic hatching egg identification equipment and method |
| CN104663510B (en) * | 2015-03-16 | 2017-03-08 | 滨州学院 | Fully automated type embryo egg testing equipment |
| CN108575820B (en) * | 2018-03-27 | 2019-07-16 | 浙江大学 | A kind of hatching egg non-damage drive device and method |
| CN112220449B (en) * | 2019-07-15 | 2023-09-15 | 爱科维申科技（天津）有限公司 | Photoelectric chicken embryo survival detection device and method |
| CN114128645A (en) * | 2021-10-18 | 2022-03-04 | 江苏宏盛景智能装备有限公司 | Egg AI intellectual detection system machine |
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Effective date of registration: 20231027 Address after: 300450 Tianjin Binhai New Area Free Trade Pilot Zone (Airport Economic Zone) 278 Hangkong Road, Block B Office Building, 1st Floor Patentee after: AIKE WEISHEN SCIENCE & TECHNOLOGY (TIANJIN) CO.,LTD. Address before: No. 399, Binshui West Road, Xiqing District, Tianjin Patentee before: TIANJIN POLYTECHNIC University |