CN109431484B - Device and method for identifying gender of egg embryo based on heart rate measurement - Google Patents
Device and method for identifying gender of egg embryo based on heart rate measurement Download PDFInfo
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000009532 heart rate measurement Methods 0.000 title claims abstract description 15
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- 210000003278 egg shell Anatomy 0.000 claims abstract description 49
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 32
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 32
- 238000012545 processing Methods 0.000 claims abstract description 26
- 230000033001 locomotion Effects 0.000 claims abstract description 9
- 230000005540 biological transmission Effects 0.000 claims description 16
- 230000003068 static effect Effects 0.000 claims description 14
- 210000003743 erythrocyte Anatomy 0.000 claims description 10
- 210000002257 embryonic structure Anatomy 0.000 claims description 9
- 238000003384 imaging method Methods 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 7
- 210000004204 blood vessel Anatomy 0.000 claims description 6
- 230000020509 sex determination Effects 0.000 claims description 6
- 238000007781 pre-processing Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 4
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 230000012447 hatching Effects 0.000 abstract description 16
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- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 description 12
- 235000013330 chicken meat Nutrition 0.000 description 10
- 238000005457 optimization Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
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- 230000000694 effects Effects 0.000 description 2
- 229940011871 estrogen Drugs 0.000 description 2
- 239000000262 estrogen Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/024—Detecting, measuring or recording pulse rate or heart rate
- A61B5/02411—Detecting, measuring or recording pulse rate or heart rate of foetuses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
- A61B5/7235—Details of waveform analysis
- A61B5/7253—Details of waveform analysis characterised by using transforms
- A61B5/7257—Details of waveform analysis characterised by using transforms using Fourier transforms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
- A61B5/7271—Specific aspects of physiological measurement analysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
Abstract
The application discloses a device and a method for identifying the sex of an egg embryo based on heart rate measurement, wherein the device comprises the following steps: the eggshell picking and placing device comprises a conveying module, a laser module, an eggshell picking and placing module, a light source module, an image acquisition module, a control module and a computer processing terminal, wherein the control module is respectively connected with the laser module, the eggshell picking and placing module and the computer processing terminal, the laser module, the eggshell picking and placing module and the image acquisition module are all located above the conveying module and are sequentially arranged along the movement direction of the conveying module, and the light source module is located between the image acquisition module and the conveying module. The sex of the eggs is judged by utilizing the heart rate value of the egg hatching stage, so that the eggs with different sexes are reasonably processed according to production requirements, the production benefit of the chicken raising industry is effectively improved, a large number of roosters with zero day age are prevented from being killed, ethical problems are avoided, and the method is easy and convenient to operate and is beneficial to being put into practical production environments.
Description
Technical Field
The application relates to the field of biological sex identification, in particular to a device and a method for identifying the sex of an egg embryo based on heart rate measurement.
Background
With the expansion of chicken raising scale, the problem that the sex of egg embryos is difficult to identify is more and more urgent. In chicken farms, the number of cocks serving as one of the production tools of fertilized eggs is very small, the sex is judged according to the physical characteristics of the chicks after hatching, and many chicken farms directly live-embed, drown or poison-kill the cocks just after hatching, and the like, and the cocks are killed as waste live-action, so that the means are very forgiving, and ethical problems exist. Therefore, it is necessary to sexing chick embryos at an early stage of their development.
Patent CN201510622442.4 proposes a sex identification method of chicken embryo eggs, which identifies the sex of the chicken embryo by measuring the content of estradiol E2 and the content of estrogen sulfate E1 in allantoic fluid in the chicken embryo. However, the method for identifying the sex of the egg embryo based on the estrogen content in the allantoic fluid needs to prick the extracting device into the egg embryo to collect the allantoic fluid in the egg embryo, the extracting process possibly causes damage to the egg embryo, the subsequent hatching is affected, the survival rate is reduced, and the collecting operation is complex.
Patent CN100397077C proposes a method and apparatus for determining the sex of fertilized eggs by acquiring images of the eggs, obtaining two-dimensional profile image data of the eggs, extracting one or more parameters representing the shape characteristics of the fertilized eggs, and performing sex determination. However, the egg shape is complex, the egg shape parameter type data are more, a large amount of data are required to be collected to analyze the sex of the eggs, and the collection and analysis processes are complex.
The two methods for detecting the sex of the egg embryo are complicated in operation steps, complex in detection process, and unsuitable for mass production because the egg embryo is damaged in the process of collection and detection.
Disclosure of Invention
Aiming at the problems in the prior art, the application provides a device and a method for identifying the sex of an egg embryo based on heart rate measurement.
The application solves the technical problems as follows: an apparatus for sexing an egg embryo based on heart rate measurements, comprising: the eggshell picking and placing device comprises a transmission module, a laser module, an eggshell picking and placing module, a light source module, an image acquisition module, a control module and a computer processing terminal, wherein the control module is respectively connected with the laser module, the eggshell picking and placing module and the computer processing terminal, the image acquisition module is connected with the computer processing terminal, the laser module, the eggshell picking and placing module and the image acquisition module are all located above the transmission module and are sequentially arranged along the movement direction of the transmission module, and the light source module is located between the image acquisition module and the transmission module;
the image acquisition module is used for acquiring images of heart areas of the eggs to be detected, converting the acquired images into electric signals and transmitting the electric signals to the computer processing terminal.
Further, the eggshell taking and placing module comprises a telescopic rod and a sucker, and the lower end of the telescopic rod is fixedly connected with the sucker.
Further, the annular rail is arranged above the telescopic rod, and the upper end of the telescopic rod is clamped with the annular rail.
Further, the image acquisition module comprises a camera and a lens, and the lens is positioned below the camera.
Further, the image acquisition module is connected with the computer processing terminal through an acquisition card, and the acquisition card is used for converting the electric signal output by the image acquisition module into a digital signal and transmitting the digital signal to the computer processing terminal.
Further, the laser module comprises a laser and a laser turning head, wherein the laser turning head is positioned at the lower end of the laser, and the laser is fixedly connected with the laser turning head.
Further, the light source module is a low-coherence light source, and the transmission module is a transmission belt.
A method for sex identification of an egg embryo based on heart rate measurement, utilizing a device for sex identification of an egg embryo based on heart rate measurement, the method comprising:
the laser module is positioned above the egg to be tested, the lower part of the laser module is provided with a laser turning head, the laser turning head emits laser, the laser turning head performs circular motion or the egg to be tested rotates, and the laser cuts the surface of the egg to be tested into holes;
the eggshell taking and placing module is used for taking away eggshells which are cut and separated in the holes;
the image acquisition module acquires images of embryo heart areas in the eggs to be detected through the holes;
the eggshell taking and placing module is used for placing the separated eggshells back to holes of the eggs to be tested;
performing adhesive restoration on the separated eggshells and the eggs to be tested;
after the acquired image is subjected to image preprocessing, calculating the heart rate value of the egg to be detected, and judging the sex of the embryo of the egg to be detected according to the obtained heart rate value.
Further, the preprocessing includes:
performing fast Fourier transform on the acquired image, performing inverse fast Fourier transform on the image to obtain a time domain signal of a dynamic signal and a time domain signal of a static signal, and taking the ratio of the dynamic signal to the static signal as an imaging parameter;
the dynamic signal is a signal of intravascular red blood cell flow in the image, the static signal is a signal of background tissue in the image, and the background tissue is a substance formed by combining extravascular cells and extravascular cell interstitials.
Further, the calculating the heart rate value of the egg to be measured includes:
and obtaining a heart rate curve chart by using imaging parameters of the preprocessed image, and calculating the number of curve peaks on the heart rate curve chart within one minute to obtain the heart rate value of the egg to be detected.
The beneficial effects of the application are as follows: according to the application, the heart rate value of the egg hatching stage is obtained through image processing, and the sex of the egg embryo is judged according to the heart rate value, so that the reasonable treatment of eggs with different sexes according to production needs is facilitated, the production benefit of the chicken raising industry is effectively improved, a large number of hatched cocks can be prevented from being killed, ethical problems are avoided, meanwhile, the operation is simple and convenient, the egg embryo is not required to be contacted in the collection and identification process, the influence on the normal hatching of the later stage of the egg is avoided, the survival rate of the egg is guaranteed, and the method is beneficial to being put into the actual production environment.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is evident that the drawings described are only some embodiments of the application, but not all embodiments, and that other designs and drawings can be obtained from these drawings by a person skilled in the art without inventive effort.
FIG. 1 is a schematic diagram of an embodiment of the present application;
FIG. 2 is a left side view of the structure of an embodiment of the present application;
FIG. 3 is a schematic diagram of the laser module, eggshell pick-and-place module and image acquisition module of the present application;
fig. 4 is a graph of heart rate of an egg to be tested in the present application.
Detailed Description
The conception, specific structure, and technical effects produced by the present application will be clearly and completely described below with reference to the embodiments and the drawings to fully understand the objects, features, and effects of the present application. It is apparent that the described embodiments are only some embodiments of the present application, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present application based on the embodiments of the present application. In addition, all connection relationships mentioned herein do not refer to direct connection of the components, but rather, refer to a connection structure that may be better formed by adding or subtracting connection aids depending on the particular implementation. The technical features in the application can be interactively combined on the premise of no contradiction and conflict.
Embodiment 1, referring to fig. 1 and 2, an apparatus for sex determination of an egg embryo based on heart rate measurement, comprising: the eggshell picking and placing system comprises a transmission module 100, a laser module 200, an eggshell picking and placing module 300, a light source module 400, an image acquisition module 500, a control module 700 and a computer processing terminal 600, wherein the control module 700 is respectively connected with the laser module 200, the eggshell picking and placing module 300 and the computer processing terminal 600, the image acquisition module 500 is connected with the computer processing terminal 600, the laser module 200, the eggshell picking and placing module 300 and the image acquisition module 500 are all positioned above the transmission module 100 and are sequentially arranged along the movement direction of the transmission module 100, and the light source module 400 is positioned between the image acquisition module 500 and the transmission module 100;
the image acquisition module 500 is used for acquiring an image of a heart region of an egg embryo to be detected, and converting the acquired image into an electric signal to be transmitted to the computer processing terminal 600.
As an optimization, referring to fig. 3, the eggshell picking and placing module 300 includes a telescopic rod 310 and a sucker 320, and the lower end of the telescopic rod 310 is fixedly connected with the sucker 320.
As an optimization, the image acquisition module 500 includes a camera 510 and a lens 520, and the lens 520 is located below the camera 510.
As an optimization, the image acquisition module 500 is connected with the computer processing terminal 600 through the acquisition card 610, and the acquisition card 610 is used for converting the electric signal output by the image acquisition module 500 into a digital signal and transmitting the digital signal to the computer processing terminal 600.
Preferably, the laser module 200 includes a laser 210 and a laser turn 220, and the laser turn 220 is located at a lower end of the laser 210. Preferably, the light source module 400 is a low-coherence light source, and the conveying module 100 is a conveyor belt.
The surface of conveyer belt is equipped with the fixing base, the fixing base is used for fixedly placing the egg that awaits measuring.
The control module 700 is a programmable logic controller, and the control module 700 is configured to control the laser module 200 to complete eggshell cutting actions of eggshells to be tested, and to control the eggshell picking and placing module 300 to complete picking and placing of eggshells separated by cutting.
The working process of the application comprises the following steps:
the application mainly detects the eggs to be detected, wherein the incubation time of the eggs is 74 to 92 hours. The conveying module 100 is used for conveying eggs to be tested, and the eggs to be tested are placed on the fixing base in a mode that big ends are at the lower ends in the transportation process. When the egg to be measured is transported to the lower side of the laser module 200, the laser turning head 220 is controlled to be adjusted to an included angle of 10 degrees with the vertical direction, the laser 210 sends out a laser beam through the laser turning head 220, the laser turning head 220 performs circular motion or the egg to be measured rotates, and the laser beam cuts a complete hole on the eggshell of the egg to be measured. The control module 700 controls the extension of the telescoping rod 310 and the suction cup 320 sucks up the eggshells separated by the laser cutting in the hole.
The low coherence light source irradiates the interior of the egg to be detected through the opened hole, the lens 520 focuses the collected blood flow signals of the region near the embryo heart of the egg to be detected to the camera 510, the camera 510 converts the received light signals into electric signals, the collection card 610 is used for converting the electric signals output by the camera 510 into digital signals and transmitting the digital signals to the computer processing terminal 600, and the computer processing terminal 600 analyzes the signals of the collected images to obtain the heart rate value of the egg to be detected.
After the image acquisition module 500 acquires the image, the control module 700 controls the eggshell taking and placing module 300 to put the separated eggshells back into the holes, and in order not to affect the subsequent incubation of the eggs, the eggs to be tested and the eggshells separated by cutting are adhered and restored by using the biocompatible adhesive tape manually.
And (3) according to the hatching time of the eggs to be tested and the obtained heart rate value, matching with the table 1, and judging the male and female of the embryos of the eggs to be tested. Refer to the following table:
TABLE 1 heart rate value statistics table corresponding to hatched times of different sexes of eggs to be tested
For example: when the hatching time of the egg to be tested is 74 hours, the detected and calculated heart rate value is 72 times/min, and the male heart rate value range of 66-78 times/min when the hatching time is 74 hours in the table 1 is satisfied, and the sex of the embryo of the egg to be tested is judged to be male.
Randomly extracting the eggs to be tested, wherein the total number of the eggs to be tested is 5000, the eggs are divided into 5 groups of 1000, the hatching time of the first group of eggs to be tested is 74 hours, the hatching time of the second group of eggs to be tested is 92 hours, the hatching time of the third group of eggs to be tested is 82 hours, the hatching time of the fourth group of eggs to be tested is 84 hours, the hatching time of the fifth group of eggs to be tested is 86 hours, heart rate value detection is carried out on the five groups of eggs to be tested respectively, the detected heart rate value is matched with the table 1, the gender of the eggs to be tested is judged, and gender distinguishing marks are made. And carrying out subsequent hatching on the marked five groups of eggs to be tested, carrying out grouped feeding on the hatched chickens according to the classified groups, judging the sex of the chickens completely through the appearance characteristics after 36 days of age, counting the number of correct sex and the number of incorrect sex according to the heart rate value, and calculating the total correct rate. The results are set forth in the following table:
table 2 heart rate value test results for determining the sex of eggs to be tested
| Sample numbering | Number of experiments | Correct number of | Number of errors | Accuracy rate of |
| First group of | 1000 | 945 | 55 | 94.5％ |
| Second group of | 1000 | 939 | 61 | 93.9％ |
| Third group of | 1000 | 941 | 59 | 94.1％ |
| Fourth group | 1000 | 945 | 55 | 94.5％ |
| Fifth group of | 1000 | 950 | 50 | 95％ |
Therefore, the sex of the egg to be tested can be judged according to the heart rate value of the egg to be tested.
As an optimization, an annular rail 330 is disposed above the telescopic rod 310, and the upper end of the telescopic rod 310 is clamped with the annular rail 330.
The eggshell taking and placing module 300 is provided with a plurality of telescopic rods 310 and suckers 320 positioned at the lower ends of the corresponding telescopic rods, after the telescopic rods 310 and the suckers 320 absorb eggshells cut and separated from eggs to be tested, the eggs to be tested are moved forwards after the image acquisition module 500 acquires images, the telescopic rods 310 move to the upper sides of the eggs to be tested along the annular track 330, and the replacement of the eggshells cut and separated from the eggs to be tested is completed. The plurality of telescopic rods 310 and the sucking disc 320 can move simultaneously, and the taking-off and the putting-back of the egg shell to be tested are not required to be carried out at the same position, so that the detection rate can be accelerated, and the flexibility is improved.
According to the application, the eggshells of the eggs to be detected are cut through the laser beams, the images of the interiors of the eggs to be detected are collected, the heart rate value of the eggs to be detected is obtained through image processing, the sex of the eggs to be detected is judged according to the heart rate value, the operation is simple and convenient, the egg embryo is not damaged due to the fact that the egg embryo is not required to be contacted in the collection and detection process, the normal hatching of the later stages of the eggs to be detected is guaranteed, the survival rate of the eggs to be detected is guaranteed, the operation is simple and convenient, and the eggs to be detected are put into an actual production environment.
Meanwhile, sex identification is carried out on the eggs to be tested in the development stage of the eggs, and different eggs can be reasonably processed according to production requirements, so that the production benefit of the chicken raising industry can be greatly improved, a large number of roosters with zero day age can be prevented from being killed, and ethical problems are avoided.
As an optimization, the device for sex identification of egg embryos based on heart rate measurement further comprises a method for sex identification of egg embryos based on heart rate measurement, the method comprising:
the laser module 200 is positioned above the egg to be tested, the lower part of the laser module 200 is provided with a laser turning head 220, the laser turning head 220 emits laser, the laser turning head 220 performs circular motion or the egg to be tested rotates, and the laser cuts a hole on the surface of the egg to be tested;
the eggshell taking and placing module 300 takes away eggshells cut and separated in the holes;
the image acquisition module 500 acquires images of embryo heart areas inside the eggs to be detected through the holes;
the eggshell taking and placing module 300 places the separated eggshells back into the holes of the eggs to be tested;
performing adhesive restoration on the separated eggshells and the eggs to be tested;
after the acquired image is subjected to image preprocessing, calculating the heart rate value of the egg to be detected, and judging the sex of the embryo of the egg to be detected according to the obtained heart rate value.
As an optimization, the preprocessing process comprises the following steps:
performing fast Fourier transform on the acquired image, performing inverse fast Fourier transform on the image to obtain a time domain signal of a dynamic signal and a time domain signal of a static signal, and taking the ratio of the dynamic signal to the static signal as an imaging parameter;
the dynamic signal is a signal of intravascular red blood cell flow in the image, the static signal is a signal of background tissue in the image, and the background tissue is a substance formed by combining extravascular cells and extravascular cell interstitials.
As optimization, the process of calculating the heart rate value of the egg to be tested comprises the following steps:
and obtaining a heart rate curve graph by using imaging parameters of the preprocessed image, and calculating the number of cycles on the heart rate curve graph within one minute to obtain the heart rate value of the egg to be detected.
The image processing process and the process for calculating the heart rate value of the egg to be detected comprise the following steps:
the signal corresponding to any pixel point of the acquired image by the image acquisition module 500 may be expressed as:
I(t)＝I 0 +I N (t)+I RBC (t) (1)
I 0 intensity of scattered light generated for background tissue, I N (t) is noise signal strength, I RBC And (t) is the intensity of scattered light produced by the moving red blood cells. As red blood cells pass, the I (t) value decreases. The background tissue is a substance that combines cells and interstitial spaces other than blood vessels. Performing fast fourier transform on I (t):
wherein f i Modulation frequency introduced for red blood cell movement. The movement of red blood cells causes the signal intensity at the vascular site to rise and fall over time, which can be seen as an ac signal, and the signal at the background tissue as a dc signal. The dynamic signal is a dynamic signal of red blood cell flow in a blood vessel, and the static signal is a signal at background tissue.
Thus, the ratio of dynamic information to static information is defined as an imaging parameter, and there are:
wherein { FFT } is t→f [I(t)} fm1～fm2 At a frequency f m1 ～f m2 Average intensity of signals in range, extracting the partial frequency domain signal to achieve imaging of small vessels, { FFT t→f [I(t)]} fv1～fv2 At a frequency f v1 ～f v2 The average intensity of the signals in the range, the part of the frequency domain signal is extracted to realize the imaging of the main blood vessel, and the two parts reflect dynamic information. FFT (fast Fourier transform) t→f [I(t)] f＝0 Is zero frequency signal value and reflects static information. N is the number of selected frequency regions. By selecting the modulation frequency range, clear images of small blood vessels and main blood vessels can be obtained at the same time.
Inverse fast fourier transforming the dynamic signal and the static signal:
{I d (x,y)} t ＝iFFT({FFT t→f [I(t)]} fm1～fm2 +{FFT t→f [I(t)]} fv1～fv2 )
{I s (x,y)} t ＝iFFT(N×FFT t→f [I(t)] f＝0 )
the above formula is { I } d (x,y)} t To obtain a time domain signal after performing inverse fast Fourier transform on the dynamic signal, and { I } s (x,y)} t And obtaining a time domain signal after carrying out inverse fast Fourier transform on the static signal, wherein x and y are the coordinates of the pixel point in the image respectively.
M is a time domain signal obtained by inverse fast fourier transforming MD.
ST＝mean(mean(M(a:b,c:d,T))) (5)
Wherein ST is the intensity of light, a, b, c, d are the coordinates of two pixels (c, a) and (d, b) of the selected region, respectively, T is time, and then a heart rate graph is obtained according to formulas (5) and M. Referring to fig. 4, the heart rate value of the egg to be tested is obtained by calculating the number of periods in one minute.
And (3) performing image processing and analysis on the acquired image inside the egg to be tested to obtain a heart rate value of the egg to be tested, and judging the gender of the egg to be tested according to the heart rate value and the hatched time of the egg to be tested.
While the preferred embodiment of the present application has been described in detail, the application is not limited to the embodiments, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the application, and these modifications and substitutions are intended to be included in the scope of the present application as defined in the appended claims.
Claims (3)
1. An apparatus for sex determination of egg embryos based on heart rate measurement, characterized by being applied to a method for sex determination of egg embryos based on heart rate measurement, for detecting eggs to be tested which have been hatched for 74 hours to 92 hours, the apparatus comprising: the eggshell picking and placing device comprises a transmission module, a laser module, an eggshell picking and placing module, a light source module, an image acquisition module, a control module and a computer processing terminal;
the control module is respectively connected with the laser module, the eggshell picking and placing module and the computer processing terminal, the image acquisition module is connected with the computer processing terminal, the laser module, the eggshell picking and placing module and the image acquisition module are all positioned above the transmission module and are sequentially arranged along the movement direction of the transmission module, and the light source module is positioned between the image acquisition module and the transmission module;
the image acquisition module is used for acquiring images of heart areas of the eggs to be detected, converting the acquired images into electric signals and transmitting the electric signals to the computer processing terminal;
the eggshell taking and placing module comprises a telescopic rod and a sucker, and the lower end of the telescopic rod is fixedly connected with the sucker;
an annular rail is arranged above the telescopic rod, and the upper end of the telescopic rod is clamped with the annular rail;
the laser module comprises a laser and a laser turning head, the laser turning head is positioned at the lower end of the laser, and the laser is fixedly connected with the laser turning head;
the light source module is a low-coherence light source, and the transmission module is a transmission belt;
the method comprises the following steps:
the laser module is positioned above the egg to be tested, the lower part of the laser module is provided with a laser turning head, the laser turning head emits laser, the laser turning head is adjusted to form an included angle of 10 degrees with the vertical direction, the laser turning head performs circular motion or the egg to be tested rotates, and the laser cuts the surface of the egg to be tested into holes;
the eggshell taking and placing module is used for taking away eggshells which are cut and separated in the holes;
the image acquisition module acquires images of embryo heart areas in the eggs to be detected through the holes;
the eggshell taking and placing module is used for placing the separated eggshells back to holes of the eggs to be tested;
performing adhesive restoration on the separated eggshells and the eggs to be tested;
after image preprocessing is carried out on the acquired images, heart rate values of eggs to be detected are calculated, and the gender of the embryos of the eggs to be detected is judged according to the obtained heart rate values;
the pretreatment comprises the following steps:
performing fast Fourier transform on the acquired image, performing inverse fast Fourier transform on the image to obtain a time domain signal of a dynamic signal and a time domain signal of a static signal, and taking the ratio of the dynamic signal to the static signal as an imaging parameter;
the dynamic signal is a signal of blood vessel red blood cell flowing in the image, the static signal is a signal of background tissue in the image, and the background tissue is a substance formed by combining extravascular cells and extravascular cell interstitials;
the calculating of the heart rate value of the egg to be measured comprises:
and obtaining a heart rate curve chart by using imaging parameters of the preprocessed image, and calculating the number of curve peaks on the heart rate curve chart within one minute to obtain the heart rate value of the egg to be detected.
2. An apparatus for sex determination of egg embryos based on heart rate measurement as claimed in claim 1, wherein: the image acquisition module comprises a camera and a lens, and the lens is positioned below the camera.
3. An apparatus for sex determination of egg embryos based on heart rate measurement as claimed in claim 2, wherein: the image acquisition module is connected with the computer processing terminal through an acquisition card, and the acquisition card is used for converting the electric signal output by the image acquisition module into a digital signal and transmitting the digital signal to the computer processing terminal.
Priority Applications (1)
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| CN112168138B (en) * | 2019-07-02 | 2023-04-14 | 爱科维申科技（天津）有限公司 | Egg embryo viability classification method based on FCN and GRU |
| CN110751178A (en) * | 2019-09-17 | 2020-02-04 | 佛山科学技术学院 | Egg shape characteristic parameter-based method and device for identifying gender of egg embryo |
| CN110771533B (en) * | 2019-09-29 | 2022-03-01 | 北京农业智能装备技术研究中心 | System and method for detecting gender of embryonated egg |
| CN111297342A (en) * | 2019-11-21 | 2020-06-19 | 佛山科学技术学院 | Heart rhythm measuring method based on egg embryo blood flow information |
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