CN109431484A - A kind of device and method of the egg Embryo sexing based on heart rate measurement - Google Patents
A kind of device and method of the egg Embryo sexing based on heart rate measurement Download PDFInfo
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000009532 heart rate measurement Methods 0.000 title claims abstract description 22
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 45
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 45
- 210000003278 egg shell Anatomy 0.000 claims abstract description 45
- 238000012545 processing Methods 0.000 claims abstract description 24
- 230000033001 locomotion Effects 0.000 claims abstract description 6
- 238000005520 cutting process Methods 0.000 claims description 11
- 210000001519 tissue Anatomy 0.000 claims description 10
- 238000003384 imaging method Methods 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 7
- 210000003743 erythrocyte Anatomy 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 claims description 4
- 210000000805 cytoplasm Anatomy 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000007781 pre-processing Methods 0.000 claims description 3
- 230000012447 hatching Effects 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 244000144977 poultry Species 0.000 abstract description 4
- 235000013601 eggs Nutrition 0.000 description 106
- 230000008569 process Effects 0.000 description 10
- 238000005457 optimization Methods 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 8
- 210000004204 blood vessel Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940011871 estrogen Drugs 0.000 description 2
- 239000000262 estrogen Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 208000004141 microcephaly Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/024—Detecting, measuring or recording pulse rate or heart rate
- A61B5/02411—Detecting, measuring or recording pulse rate or heart rate of foetuses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
- A61B5/7235—Details of waveform analysis
- A61B5/7253—Details of waveform analysis characterised by using transforms
- A61B5/7257—Details of waveform analysis characterised by using transforms using Fourier transforms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
- A61B5/7271—Specific aspects of physiological measurement analysis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The device and method of the invention discloses a kind of egg Embryo sexing based on heart rate measurement, it include: delivery module, laser module, eggshell pick-and-place module, light source module, image capture module, control module and computer processing terminal, the control module picks and places module with laser module, eggshell respectively, computer processing terminal is connected, laser module, eggshell pick and place module and image capture module is respectively positioned on the top of delivery module, and set gradually along the direction of motion of delivery module, the light source module is between image capture module and delivery module.The present invention judges the gender of egg using the heart rate value in egg hatching stage, be conducive to according to production needs rationally handle the egg of different sexes in this way, not only effectively improve the productivity effect of poultry husbandry, and it avoids killing the cock cruelty of a large amount of zero ages in days, avoid ethics morals problem, it is easy to operate simultaneously, be conducive to put into actual production environment.
Description
Technical field
The present invention relates to biological sex identification fields, more specifically to a kind of egg Embryo based on heart rate measurement
The device and method that do not identify.
Background technique
With the expansion of poultry scale, it is now to solve the problems, such as that egg embryo gender is difficult to identify more more and more urgent.?
In chicken farm, cock is only used as needed for one of tool of production of fertile egg quantity seldom, and the prior art is according to the young bird after hatching
The physical trait of chicken judges gender, and many chicken farms are just directly directly buried alive, be drowned or poisoned with poison come cock soon just hatching
Deng being killed while still alive as waste, means are very cruel, and there are ethics morals problems.Therefore, in chick embryo development early stage
Sex identification just is carried out to it, is necessary.
Patent CN201510622442.4 proposes a kind of embryo in egg sex appraisal method, and this method passes through measurement egg embryo
Estradiol E2 content and estrogen sulfate E1 content identify the gender of egg embryo in allantoic fluid in tire.But it is this to be based on
The method that estrogen content identifies egg embryo gender in allantoic fluid needs to penetrate draw-out device into egg embryo to adopt
Collect the intraembryonic allantoic fluid of egg, the process of extraction is possible to that egg embryo can be damaged, and influences subsequent hatching, reduces
Survival rate, while acquiring complicated for operation.
Patent CN100397077C proposes the method and apparatus for the gender for determining the egg of fertilization, by acquiring chicken
The image of egg obtains the two-dimensional silhouette image data of egg, extracts the one or more for indicating the shape feature of the fertilized eggs
Parameter carries out gender and determines.But egg type is more complicated, egg type parameter type data are more, need to acquire mass data analysis
The gender of egg, acquisition and analytic process are more complicated.
Above-described two kinds of detections egg Embryo method for distinguishing, operating procedure is excessively cumbersome, and detection process is more complex,
And egg embryo is caused to damage during acquisition testing, is not suitable for largely putting into production.
Summary of the invention
Aiming at the problems existing in the prior art, the present invention provides a kind of egg Embryo sexing based on heart rate measurement
Device and method, the heart rate value of egg embryo is collected by image procossing, egg embryo gender is judged according to heart rate value.
The solution that the present invention solves its technical problem is: a kind of egg Embryo sexing based on heart rate measurement
Device, comprising: delivery module, laser module, eggshell pick and place module, light source module, image capture module, control module and computer
Processing terminal, the control module picks and places module with laser module, eggshell respectively, computer processing terminal is connected, described image
Acquisition module is connected with computer processing terminal, and the laser module, eggshell pick and place module and image capture module is respectively positioned on institute
The top of delivery module is stated, and is set gradually along the direction of motion of delivery module, the light source module is located at image capture module
Between the delivery module；
Described image acquisition module is used to acquire the image in egg embryo heart to be measured region, and acquisition gained image is turned
It is changed to electric signal and passes to computer processing terminal.
Further, it includes telescopic rod and sucker that the eggshell, which picks and places module, and the lower end of the telescopic rod and sucker are affixed.
Further, the top of the telescopic rod is equipped with circular orbit, and the upper end of telescopic rod and circular orbit are clamped.
Further, described image acquisition module includes camera and camera lens, and the camera lens is located at the lower section of camera.
Further, described image acquisition module is connected by capture card with computer processing terminal, and the capture card is used for
The electric signal that image capture module exports is converted to digital signal, and gives the digital data transmission to computer processing terminal.
Further, the laser module includes laser and laser rotary head, and the laser rotary head is located at the laser
Lower end, the laser are fixedly connected with the laser rotary head.
Further, the light source module is low-coherent light light source, and the delivery module is transmission belt.
A method of the egg Embryo sexing based on heart rate measurement utilizes a kind of egg embryo based on heart rate measurement
The device of tire sex identification, which comprises
Laser module is located at the top of egg to be measured, and the lower part of laser module is equipped with laser rotary head, and laser rotary head, which issues, to swash
Light, laser rotary head moves in a circle or egg to be measured rotation, and laser portals the surface cutting of egg to be measured；
Eggshell picks and places module and takes the eggshell for cutting separation in hole away；
Image capture module acquires the image in embryo heart region inside egg to be measured by the hole；
Eggshell picks and places the hole that isolated eggshell is put back to egg to be measured by module；
Bonding recovery is carried out to isolated eggshell and egg to be measured；
After acquiring resulting image progress image preprocessing, the heart rate value of egg to be measured is calculated, according to the heart obtained
Rate value judges egg embryo gender to be measured.
Further, described pre-process includes:
Fast Fourier Transform (FFT) is carried out to resulting image is acquired, then reverse Fast Fourier Transform (FFT) is carried out to image, is obtained
To the time-domain signal of Dynamic Signal and stationary singnal, and using the ratio between Dynamic Signal and stationary singnal as imaging parameter；
The Dynamic Signal is the signal of erythrocyte flowing in image medium vessels, and the stationary singnal is background in image
Signal at tissue, the background tissues are the substance that extravascular cell and extravascular cytoplasm combine.
Further, the heart rate value for calculating egg to be measured includes:
Heart rate curve graph is obtained using the imaging parameter of pretreated image, then calculates the heart rate curve in one minute
The number at figure upper curve peak, obtains the heart rate value of egg to be measured.
The beneficial effects of the present invention are: the present invention obtains the heart rate value in egg hatching stage by image procossing, according to the heart
Rate value judges the gender of egg embryo, is conducive to according to production needs rationally handle the egg of different sexes in this way, no
The productivity effect of poultry husbandry is only effectively improved, can be killed to avoid the cock cruelty that will largely hatch, ethics is avoided to ask
Topic, at the same it is easy to operate, and acquisition qualification process does not need contact egg embryo, avoids the normal incubation for influencing the egg later period, has
Conducive to the survival rate for ensureing egg, be conducive to put into actual production environment.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is a part of the embodiments of the present invention, rather than is all implemented
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is the structural schematic diagram of the embodiment of the present invention；
Fig. 2 is the left view of the embodiment of the present invention structure；
Fig. 3 is the structural schematic diagram of laser module of the invention, eggshell pick-and-place module and image capture module；
Fig. 4 is egg heart rate curve graph to be measured in the present invention.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to design of the invention, specific structure and generation clear
Chu, complete description, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of the embodiment, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art are not being paid
Other embodiments obtained, belong to the scope of protection of the invention under the premise of creative work.In addition, be previously mentioned in text
All connection relationships not singly refer to that component directly connects, and referring to can be according to specific implementation situation, by adding or reducing connection
Auxiliary, Lai Zucheng more preferably connection structure.Each technical characteristic in the invention, under the premise of not conflicting conflict
It can be with combination of interactions.
Embodiment 1, referring to Figures 1 and 2, a kind of device of the egg Embryo sexing based on heart rate measurement, comprising: pass
Module 100, laser module 200, eggshell is sent to pick and place module 300, light source module 400, image capture module 500, control module 700
With computer processing terminal 600, the control module 700 picks and places module 300, computer processing with laser module 200, eggshell eventually respectively
End 600 is connected, and described image acquisition module 500 is connected with computer processing terminal 600, and the laser module 200, eggshell take
Amplification module 300 and image capture module 500 are respectively positioned on the top of the delivery module 100, and along the movement side of delivery module 100
To setting gradually, the light source module 400 is between image capture module 500 and the delivery module 100；
Described image acquisition module 500 is used to acquire the image in egg embryo heart to be measured region, and will acquisition gained figure
Computer processing terminal 600 is passed to as being converted to electric signal.
As optimization, with reference to Fig. 3, it includes telescopic rod 310 and sucker 320, the telescopic rod that the eggshell, which picks and places module 300,
310 lower end and sucker 320 are affixed.
As optimization, described image acquisition module 500 includes camera 510 and camera lens 520, and the camera lens 520 is located at camera
510 lower section.
As optimization, described image acquisition module 500 is connected by capture card 610 with computer processing terminal 600, described
Capture card 610 is used to the electric signal that image capture module 500 exports being converted to digital signal, and by the digital data transmission
To computer processing terminal 600.
As optimization, the laser module 200 includes laser 210 and laser rotary head 220, the laser rotary head 220
In the lower end of the laser 210.As optimization, the light source module 400 is low-coherent light light source, the delivery module 100
For conveyer belt.
The surface of the conveyer belt is equipped with fixing seat, and the fixing seat is used for fixed placement egg to be measured.
The control module 700 is programmable logic controller (PLC), and the control module 700 is for controlling laser module 200
The eggshell cutting action of eggshell to be measured is completed, and picks and places the completion of module 300 by the isolated eggshell of cutting for controlling eggshell
It takes away and puts back to.
The course of work of the present invention:
The present invention predominantly detects the egg to be measured that brooding time is 74 hours to 92 hours.Delivery module 100 is for passing
Egg to be measured is sent, egg to be measured is to be placed in fixing seat with major part in mode of the upper microcephaly under during transportation.When
When egg to be measured is transported to the lower section of laser module 200, the control tune of laser rotary head 220 set to the angle of vertical direction in 10
Degree, laser 210 issue laser beam by laser rotary head 220, and laser rotary head 220 moves in a circle or egg to be measured rotation,
Laser beam is cut into a complete hole on the eggshell of egg to be measured.Control module 700 controls telescopic rod 310 and extends, and inhales
Disk 320 will be cut by laser isolated eggshell in hole and be picked up.
Low-coherence light source is irradiated to the inside of egg to be measured by open hole, and camera lens 520 is by collected egg to be measured
The blood flow signal of embryo heart near zone focuses on camera 510, and the optical signal received is converted electric signal by camera 510,
The capture card 610 is used to for the electric signal that camera 510 exports being converted to digital signal, and by the digital data transmission to electricity
The signal of brain processing terminal 600,600 pairs of computer processing terminal acquisition gained images is analyzed, and the heart rate of egg to be measured is obtained
Value.
After image capture module 500 has acquired image, control module 700 controls eggshell and picks and places module 300 for the separation
Eggshell put back in hole, in order to not influence the subsequent hatching of egg, by manually using biocompatibility adhesive tape by egg to be measured
Bonding recovery is carried out with the eggshell for cutting isolated.
According to the brooding time of egg to be measured and obtained heart rate value matching list 1, the male and female of egg embryo to be measured are judged.
With reference to following table:
The corresponding heart rate value statistical form of brooding time of the egg different sexes to be measured of table 1
Such as: when brooding time is 74 small to egg to be measured, detecting and calculating resulting heart rate value is 72 beats/min
When, meet 66~78 beats/min of male heart rate value range when brooding time is 74 hours in table 1, then judges the chicken to be measured
Egg embryo gender is male.
Egg to be measured is randomly selected, 5000 in total, is divided into 5 groups, every group 1000, the hatching of first group of egg to be measured
Time is 74 hours, and the brooding time of second group of egg to be measured is 92 hours, and the brooding time of third group egg to be measured is
82 hours, the brooding time of the 4th group of egg to be measured was 84 hours, and the brooding time of the 5th group of egg to be measured is 86 hours,
This five groups progress heart rate value detections to be measured respectively, and the heart rate value that will test out is matched with table 1, judges egg to be measured
Gender, and carry out the label of gender differentiation.This five groups marked eggs to be measured are subjected to subsequent hatchings, by what is hatched
Chicken is grouped raising according to the group divided, and after 36 ages in days, can determine the gender of chicken by macroscopic features completely,
Statistics judges the correct quantity of gender and number of errors according to heart rate value, calculates total accuracy.As a result as in the table below:
2 heart rate value of table judges the experimental result of egg gender to be measured
| Sample number into spectrum | Test number | Correct number | Number of errors | Accuracy |
| First group | 1000 | 945 | 55 | 94.5% |
| Second group | 1000 | 939 | 61 | 93.9% |
| Third group | 1000 | 941 | 59 | 94.1% |
| 4th group | 1000 | 945 | 55 | 94.5% |
| 5th group | 1000 | 950 | 50 | 95% |
Therefore it may determine that the gender of egg to be measured according to the heart rate value of egg to be measured.
As optimization, the top of the telescopic rod 310 is equipped with circular orbit 330, and the upper end of telescopic rod 310 and circular rails
Road 330 is clamped.
The eggshell picks and places module 300 and is equipped with several telescopic rods 310 and the sucker positioned at corresponding telescopic rod lower end
320, after telescopic rod 310 and sucker 320 have drawn the eggshell that egg cutting to be measured is separated, the egg to be measured is by Image Acquisition
It after module 500 has acquired image, travels forward, telescopic rod 310 moves to the upper of the egg to be measured along circular orbit 330
Side completes putting back to for the eggshell that cutting is separated.Several telescopic rods 310 and sucker 320 can move simultaneously, egg to be measured
Taking away and putting back to for cutting separation eggshell does not need to carry out in the same position, can accelerate detection rates, improve flexibility.
The present invention cuts the eggshell of egg to be measured by laser beam, acquires the image inside egg to be measured, passes through image
Processing obtains the heart rate value of egg to be measured, the gender of egg to be measured is judged according to heart rate value, easy to operate, acquisition testing process is not
It needs to contact egg embryo, egg embryo will not be damaged, be conducive to the normal incubation for ensureing the egg later period to be measured, ensure chicken to be measured
The survival rate of egg, it is easy to operate, be conducive to put into actual production environment.
The present invention carries out sex identification to it in the egg stage of development to be measured simultaneously, can be according to production needs to dissimilarity
Other egg is rationally handled, and the productivity effect of poultry husbandry not only can be greatly improved, can be to avoid by a large amount of zero days
The cock cruelty in age is killed, and ethics morals problem is avoided.
As optimization, the device of the egg Embryo sexing based on heart rate measurement further includes a kind of based on heart rate measurement
The method of egg Embryo sexing, which comprises
Laser module 200 is located at the top of egg to be measured, and the lower part of laser module 200 is equipped with laser rotary head 220, and laser turns
First 220 issue laser, and laser rotary head 220 moves in a circle or the surface of egg to be measured is cut by egg to be measured rotation, laser
Hole；
Eggshell picks and places module 300 and takes the eggshell for cutting separation in hole away；
Image capture module 500 acquires the image in embryo heart region inside egg to be measured by the hole；
Eggshell picks and places the hole that isolated eggshell is put back to egg to be measured by module 300；
Bonding recovery is carried out to isolated eggshell and egg to be measured；
After acquiring resulting image progress image preprocessing, the heart rate value of egg to be measured is calculated, according to the heart obtained
Rate value judges egg embryo gender to be measured.
As optimization, the pretreated process includes:
Fast Fourier Transform (FFT) is carried out to resulting image is acquired, then reverse Fast Fourier Transform (FFT) is carried out to image, is obtained
To the time-domain signal of Dynamic Signal and stationary singnal, and using the ratio between Dynamic Signal and stationary singnal as imaging parameter；
The Dynamic Signal is the signal of erythrocyte flowing in image medium vessels, and the stationary singnal is background in image
Signal at tissue, the background tissues are the substance that extravascular cell and extravascular cytoplasm combine.
As optimization, the process of the heart rate value for calculating egg to be measured includes:
Heart rate curve graph is obtained using the imaging parameter of pretreated image, then calculates the heart rate curve in one minute
The number for scheming the upper period, obtains the heart rate value of egg to be measured.
Image processing process and the process of heart rate value for calculating egg to be measured include:
Image capture module 500 acquires signal corresponding to any pixel point of resulting image and can indicate are as follows:
I (t)=I0+IN(t)+IRBC(t) (1)
I0For the scattered light intensity that background tissues generate, INIt (t) is noise signal strength, IRBCIt (t) is the blood red thin of movement
The scattered light intensity that born of the same parents generate.When erythrocyte passes through, I (t) value reduces.The background tissues are the cell in addition to blood vessel
The substance to combine with cytoplasm.Fast Fourier Transform (FFT) is carried out to I (t):
Wherein, fiIntroduced modulating frequency is moved for erythrocyte.The movement of erythrocyte causes at vessel position
Signal strength is gone up and down at any time, which can see AC signal as, and the signal at background tissues can see direct current signal as.Institute
The Dynamic Signal that Dynamic Signal is erythrocyte flowing in blood vessel is stated, the stationary singnal is the signal at background tissues.
In this way, being defined as imaging parameter with the ratio between multidate information and static information, then have:
Wherein, { FFTt→f[I(t)}Fm1~fm2It is frequency in fm1~fm2The mean intensity of signal in range extracts the portion
Dividing frequency-region signal is the imaging in order to realize thin vessels, { FFTt→f[I(t)]}Fv1~fv2It is frequency in fv1~fv2Letter in range
Number mean intensity, extracting the part frequency-region signal is the imaging in order to realize major blood vessel, this two parts, which reflects dynamic, to be believed
Breath.FFTt→f[I(t)]F=0For zero frequency signal value, reflect static information.N is the quantity of selected frequency field.Pass through selection
Modulation frequency range can obtain clearly thin vessels and major blood vessel image simultaneously.
Reverse Fast Fourier Transform (FFT) is carried out to Dynamic Signal and stationary singnal:
{Id(x,y)}t=iFFT ({ FFTt→f[I(t)]}Fm1~fm2+{FFTt→f[I(t)]}Fv1~fv2)
{Is(x,y)}t=iFFT (N × FFTt→f[I(t)]F=0)
Above formula is { Id(x,y)}tTo obtain time-domain signal, and { I after Dynamic Signal is carried out reverse Fast Fourier Transform (FFT)s
(x,y)}tTo obtain time-domain signal after stationary singnal is carried out reverse Fast Fourier Transform (FFT), wherein x, y are the pixel respectively
Coordinate in the picture.
M is the time-domain signal that will be obtained after the reverse Fast Fourier Transform (FFT) of MD.
ST=mean (mean (M (a:b, c:d, T))) (5)
Wherein ST is light intensity, a, b, c, d be respectively selected areas two pixels (c, a) and the coordinate of (d, b),
T is the time, then is worth rate curve graph of uniting as one according to formula (5) and M.With reference to Fig. 4, by calculating the period in one minute
Number, to obtain the heart rate value of egg to be measured.
By carrying out image procossing and analysis to the image acquired inside resulting egg to be measured, the heart of egg to be measured is obtained
Rate value judges the gender of egg to be measured according to the heart rate value of egg to be measured and brooding time.
Better embodiment of the invention is illustrated above, but the invention is not limited to the implementation
Example, those skilled in the art can also make various equivalent modifications on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (10)
1. a kind of device of the egg Embryo sexing based on heart rate measurement, it is characterised in that: include: delivery module, laser
Module, eggshell pick and place module, light source module, image capture module, control module and computer processing terminal, the control module point
Module is not picked and placed with laser module, eggshell, computer processing terminal is connected, described image acquisition module and computer processing terminal phase
Connection, the laser module, eggshell pick and place module and image capture module is respectively positioned on the top of the delivery module, and along transmission
The direction of motion of module is set gradually, and the light source module is between image capture module and the delivery module；
Described image acquisition module is used to acquire the image in egg embryo heart to be measured region, and acquisition gained image is converted to
Electric signal passes to computer processing terminal.
2. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 1, it is characterised in that:
It includes telescopic rod and sucker that the eggshell, which picks and places module, and the lower end of the telescopic rod and sucker are affixed.
3. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 2, it is characterised in that:
The top of the telescopic rod is equipped with circular orbit, and the upper end of telescopic rod and circular orbit are clamped.
4. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 3, it is characterised in that:
Described image acquisition module includes camera and camera lens, and the camera lens is located at the lower section of camera.
5. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 4, it is characterised in that:
Described image acquisition module is connected by capture card with computer processing terminal, and the capture card is used for image capture module is defeated
Electric signal out is converted to digital signal, and gives the digital data transmission to computer processing terminal.
6. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 5, it is characterised in that:
The laser module includes laser and laser rotary head, and the laser rotary head is located at the lower end of the laser, the laser
It is fixedly connected with the laser rotary head.
7. a kind of device of egg Embryo sexing based on heart rate measurement according to claim 6, it is characterised in that:
The light source module is low-coherent light light source, and the delivery module is transmission belt.
8. a kind of method of the egg Embryo sexing based on heart rate measurement, it is characterised in that: using in claim 6 or 7
A kind of device of the egg Embryo sexing based on heart rate measurement, which comprises
Laser module is located at the top of egg to be measured, and the lower part of laser module is equipped with laser rotary head, and laser rotary head issues laser, swashs
Light rotary head moves in a circle or egg to be measured rotation, and laser portals the surface cutting of egg to be measured；
Eggshell picks and places module and takes the eggshell for cutting separation in hole away；
Image capture module acquires the image in embryo heart region inside egg to be measured by the hole；
Eggshell picks and places the hole that isolated eggshell is put back to egg to be measured by module；
Bonding recovery is carried out to isolated eggshell and egg to be measured；
After acquiring resulting image progress image preprocessing, the heart rate value of egg to be measured is calculated, according to the heart rate value obtained
Judge egg embryo gender to be measured.
9. a kind of method of egg Embryo sexing based on heart rate measurement according to claim 8, it is characterised in that:
The pretreatment includes:
Fast Fourier Transform (FFT) is carried out to resulting image is acquired, then reverse Fast Fourier Transform (FFT) is carried out to image, is moved
The time-domain signal of state signal and stationary singnal, and using the ratio between Dynamic Signal and stationary singnal as imaging parameter；
The Dynamic Signal is the signal of erythrocyte flowing in image medium vessels, and the stationary singnal is background tissues in image
The signal at place, the background tissues are the substance that extravascular cell and extravascular cytoplasm combine.
10. a kind of method of egg Embryo sexing based on heart rate measurement according to claim 9, feature exist
In: the heart rate value for calculating egg to be measured includes:
Heart rate curve graph is obtained using the imaging parameter of pretreated image, then is calculated in one minute in the heart rate curve graph
The number of curve peak obtains the heart rate value of egg to be measured.
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