CN115702661A - Nutritional supplement with fatigue relieving function and preparation method and application thereof - Google Patents

Abstract

The invention relates to the technical field of foods, and provides a nutritional supplement with a fatigue relieving function, and a preparation method and application thereof. The nutritional supplement has the functions of refreshing, relieving fatigue, enhancing memory and learning ability, and improving work and learning efficiency, and comprises black tea powder, medium chain triglyceride, tea theanine, inulin, and compound probiotic powder; the invention also provides a nutritional supplement tablet and a preparation method thereof; also provides an application of the nutritional supplement or the nutritional supplement tablet in preparing health-care food or special medical food.

Description

Nutritional supplement with fatigue relieving function and preparation method and application thereof

Technical Field

The invention relates to the technical field of foods, in particular to a nutritional supplement with a fatigue relieving function and a preparation method and application thereof.

Background

Along with the continuous improvement of the living standard of people at present, people eat more and more abundantly, people's dietary structure has taken place some changes, and unreasonable diet leads to the number of rich and honorable diseases to increase gradually, and people begin to appear sub-health, also pay more attention to healthy diet, begin to pay attention to health care.

China faces double problems of overnutrition and nutrition deficiency on public nutrition and health, and particularly the problems of excessive weight and metabolic syndromes such as obesity, diabetes, hypertension, hyperlipidemia and the like are obvious. The comprehensive improvement of the nutrition and health of the public is actively promoted, the accurate manufacturing technical level and the development capability of the health food are continuously enhanced, and scientific and technological leads are urgently needed in aspects of nutrition balance targeted design, health intervention directional regulation, development of functional health care type nutrition and health food and special nutrition food and the like. Therefore, the development market prospect of different types of functional foods is very wide.

The development of the third generation functional food on the market is considerable, so that the analysis of the structure-effect, dose-effect relationship and action mechanism of the functional factor has important significance. Because the market of functional foods in China is relatively lagged, the application of functional factor resources to the development of functional foods in combination with the development trend of functional foods at home and abroad is an urgent need.

The candy as a more traditional industry also needs continuous innovation to meet the requirement of consumers on increasingly pursuing health and fashion. The candies are common food in life, have various varieties and different tastes, but have single components, and lack the efficacies of refreshing, restoring consciousness, relieving fatigue, enhancing memory and learning ability and improving work and learning efficiency. The nutritional supplement disclosed by the invention is rich in nutritional ingredients and good in taste, and has the health-care effects of refreshing, restoring consciousness, relieving fatigue, enhancing memory and learning capacity and improving working and learning efficiency.

The formula of the invention has the effects of refreshing, relieving fatigue, enhancing memory and learning ability, and improving work and learning efficiency.

The invention discloses a candy food with the functions of refreshing, restoring consciousness, relieving fatigue, enhancing memory and learning ability and improving work and learning efficiency, which is prepared from black tea powder, medium-chain triglyceride, tea theanine, inulin and composite probiotic bacteria powder.

Medium chain triglycerides, also known collectively as mediumchaintrigycedes, abbreviated (MCT). Fatty acid radicals are classified into short, medium and long chains according to the length of the carbon chain, and fatty acids having a carbon chain consisting of 6 to 12 carbon atoms are generally referred to as medium-chain fatty acids (MCFA), which are esterified with glycerol to form medium-chain fatty acid triglycerides (or medium-chain triglycerides, MCT). Typically MCT refers to saturated caprylic triglyceride or saturated capric triglyceride or a mixed saturated caprylic-capric triglyceride.

The medium chain triglyceride is composed of saturated fatty acids only, has low freezing point, is liquid at room temperature, and has low viscosity. Compared with soybean oil, the liquid is completely odorless and colorless transparent liquid. Compared with common oil and fat and hydrogenated oil and fat, the medium chain triglyceride has very low unsaturated fatty acid content, very high oxidation stability and iodine value not higher than 0.5. MCTs are particularly stable at high and low temperatures.

In the food additive industry, medium chain triglycerides can be used as solvents and carriers for flavorants and pigments; in baked food processing, medium chain triglycerides are often used in combination with inexpensive vegetable oils as detackifiers to prevent baked goods from sticking to pans or molds; pure medium chain triglycerides or mixed oils with vegetable oils are also commonly used as lubricants and release agents for sausage casings; the medium chain triglycerides can also be used for processing various fat soluble vitamins, pigments and antioxidants, and when small amounts of medium chain triglycerides are added, the viscosity of the high viscosity fatty substances is greatly reduced; in the food processing industry, medium chain triglycerides are also used to replace easily oxidizable milk fat for making milk liqueurs and cheese imitations; the medium chain triglycerides can also serve as clouding agents for beverages if used in combination with water soluble colloids. In addition, medium chain triglycerides can be used as lubricants in food processing machinery in place of mineral oil.

Medium Chain Triglycerides (MCT) are commonly used as special foods for patients after surgery, infection and skin burn, and also for those patients with fat malabsorption, AIDS, cancer and diabetes, and can be used for treating prostatic hyperplasia, eliminating cholelithiasis, reducing cholesterol, and preventing and treating hyperlipidemia. Besides, the MCT also can help to lose weight, and practice proves that the edible medium-chain fatty acid ester has obvious effects on the reduction of the body weight, the abdominal fat area and the waist circumference of a human body.

The black tea powder is dark brown powder, can be prepared by grinding and crushing black tea at low temperature to form fine tea powder, has higher grinding fineness, better taste and higher price, and can also be prepared by physical extraction. The black tea powder well keeps the original nutrient components, aroma, color and taste of the black tea, and the nutrients of the black tea powder are easier to be absorbed by human bodies. As a nutrition enhancer and a natural pigment additive, the tea powder is widely used in various industries such as food, health care products, cosmetics and the like, and a wide variety of tea products are derived. The caffeine in the black tea stimulates the nerve center by stimulating the cerebral cortex, promotes the refreshing and thinking concentration, further makes the thinking response more sharp and enhances the memory; it also has exciting effect on blood vessel system and heart, and can strengthen heart beat, thus accelerating blood circulation to benefit metabolism, and promoting sweating and diuresis, thereby accelerating excretion of lactic acid (substance for making muscle feel fatigue) and other old waste substances in body under double-pipe condition, and achieving the effect of relieving fatigue.

Theanine, like other amino acids, is absorbed in the small intestine, and three specific carrier systems for transporting amino acids have been identified in the small intestine wall, which transport neutral, acidic and basic amino acids, respectively. It has been experimentally shown that theanine antagonizes the same class of amino acids, such as methionine, leucine, isoleucine and valine, and it is inferred that theanine is likely to be absorbed in the intestinal tract through a methionine carrier transport system. However, kitaoka et al have shown that theanine is absorbed in the intestine by sharing a Na-coupled cotransporter with glutamate, which has a lower affinity than glutamate. The Unno et al study found that intact theanine, glutamic acid and ethylamine were detectable in the urine following theanine administration, and that these compounds were somewhat related to the amount of theanine administered. This suggests that theanine is enzymatically hydrolyzed to glutamic acid and ethylamine after absorption into the human body, and the metabolic site thereof may be the kidney. Terashima et al studied the metabolic kinetics of theanine in vivo in mice, and found that the concentration of theanine in serum, liver and brain was significantly increased 1 hour after oral gavage in mice, and thereafter gradually decreased with time, while the concentration of theanine in serum and liver reached the highest peak after 5 hours, and that theanine completely disappeared in these tissues after 24 hours.

The tea theanine has wide physiological functions.

And (4) blood pressure lowering effect. Blood pressure regulation is achieved primarily by increasing or decreasing catecholamines and 5-hydroxytryptamine in the central and peripheral nervous systems. Yokoogoshi et al have experimentally demonstrated that the administration of theanine to rats results in a reduction in the 5-hydroxytryptamine content, a reduction in brain synthesis and an increase in brain breakdown of 5-hydroxytryptamine. When different doses of theanine are injected into Spontaneous Hypertensive Rats (SHR), the blood pressure is reduced, the degree of reduction is in a dose-reflecting relationship, and the blood pressure can be obviously reduced in a high-dose group; but does not change the blood pressure of normal rats even when the highest dose of theanine is given; glutamic acid, which has a structure similar to that of theanine, is not found to have a blood pressure lowering effect.

Can assist in inhibiting tumor. Recent research shows that theanine has no antitumor activity and can raise the curative effect of various antitumor medicines. Adriamycin (adriamycin) has an inhibiting effect on partial tumors, theanine can protect the concentration of adriamycin in tumor tissues and reduce the exudation of adriamycin from epothilone ascites tumor cells, so that when the theanine is used together with the adriamycin, the growth inhibition of the living tumors can be improved by 2.1 times, and the concentration of adriamycin in the tumor tissues can be increased by 2.9 times. In addition, injection of the anticancer drug idamin (idarubicin) can reduce the number of white blood cells and bone marrow cells, but when used in combination with theanine, can alleviate the toxicity of idamin and promote the antitumor effect of idamin, which indicates that theanine has a selective regulatory effect on the toxicity caused by idamin. When the anticancer drug toxocin (doxorubicin) is used in combination with theanine, M5076 ovarian sarcoma can be inhibited from metastasis to liver. Recently, sa-dzuka et al further studied the way that theanine increases the anticancer activity of adriamycin, and indicated that theanine increases the concentration of adriamycin in tumor cells by inhibiting the transport of glutamate in tumor cells, thereby achieving an improvement in anticancer effect. Theanine is very beneficial to the clinical chemotherapy of cancer patients, not only promotes the effects of certain anti-tumor drugs, but also improves the life quality of the cancer patients.

Relaxing nervous tension, relaxing etc. Terashima et al found that the main receptor for theanine was the brain. After theanine enters the brain through brain barriers, the intragranular neurotransmitter dopamine in brain cells is obviously increased, and the dopamine is an epinephrine precursor and a norepinephrine precursor and is an important substance for conveying the excitation degree of brain nerve cells, and the release of the dopamine can greatly influence the emotion of a human. In addition, yokogoshi et al found that theanine may have an effect on brain serotonin synthesis and breakdown through the use of serotonin (5-hydroxytryptamine) metabolism inhibitors. After taking theanine, the content of tryptophan in the brain is obviously increased or tends to be increased, but the content of serotonin is reduced. Theanine may reduce serotonin synthesis and increase its breakdown, or inhibit serotonin release.

The caffeine has the functions of refreshing and exciting, the tea leaves also contain more caffeine, and the caffeine taken alone has certain harm to human bodies, and the equal amount of caffeine exciting effect cannot be generated after the tea is drunk. Mumura et al have proved that theanine has antagonistic action on the excitation of caffeine, and the results of his determination of the spontaneous movement of central nervous system caused by caffeine indicate that caffeine and theanine have obvious inhibitory action on excitation when they are administered simultaneously. Recently, it was confirmed by a brain electrograph evaluation method that theanine and caffeine antagonize the stimulatory effects of caffeine at almost the same molar concentration. These fully indicate that theanine is a caffeine antagonist and has significant sedative, tonic-reducing and neuroleptic effects.

In general, animals and humans always produce very weak impulses, called brain waves, on the surface of the brain. Under different mental conditions, brains generate brain waves with different current frequencies. Brain waves are divided into α, β, δ and θ waves according to frequency, the δ wave is when sleeping, the θ wave is when hitting a ton, the α wave is at rest (relaxed), and the β wave is when excited. Artificially verifying the influence of theanine on the mental aspect, carrying out human body experiments, drinking water and theanine water solution by testers, and measuring an electroencephalogram after 60 min; as a result, no change in electroencephalogram was observed after drinking water, and a significant α wave was generated after drinking theanine, and thus theanine has an effect of promoting generation of two waves, thereby causing a pleasant and pleasant feeling. And theanine does not cause an increase in the number of theta waves occurring in a sleep state, so theanine stabilizes human emotion and also makes attention more focused.

Has protective effect on brain nerve cells. Glutamate is excitatory neurotransmitters, and is maintained in the amount of 1mmol-10mmol/L in nerve cells. When cerebral ischemia occurs, the supply of O2 in cells is interrupted, so that the production of Adenosine Triphosphate (ATP) which is an endogenous substance in the cells is reduced and gradually exhausted, the nerve cell membranes are separated, excessive glutamate is released to the outside of the cells and acts on receptors, the concentration of calcium ions in the cells is continuously increased, various lyase activities are excited, and finally, the glutamate acts on the cells to destroy and die the cells. Theanine has competitive protection effect on glutamic acid receptor, so that theanine has protection effect on brain tissue and can prevent brain nerve cell death caused by cerebral ischemia.

Improving menstrual period syndrome. Menstrual syndrome (PMS for short) is a condition of mental and physical discomfort in women during the 3 to 10 days before menstruation. Juneeja and the like consider that theanine has an improvement effect on PMS, 24 women take 200mg of theanine every day, and PMS symptoms are obviously improved after two months, such as headache, lumbago, chest distending pain, weakness, fatigue, mental disability, dysphoria and the like. The mechanism needs further research and may be related to the sedative effect of theanine.

Has weight reducing effect. After the mice are fed with the feed mixed with 0.028% of theanine for 16 weeks, the body weight of the mice is obviously reduced compared with that of a control group, and the abdominal fat is reduced to 58% of that of the control group. Meanwhile, the content of neutral fat and cholesterol in blood is respectively reduced by 32 percent and 15 percent, and the content of cholesterol in liver is reduced by 28 percent. Therefore, the weight-reducing effect of the tea is the result of the combined action of various components including theanine, and the theanine is particularly effective in reducing cholesterol in vivo.

Has antifatigue effect. A weight swimming experiment is adopted to observe and record the swimming death time of a mouse 30 days after the mouse is orally administered with theanine, blood is collected after the mouse swims for a certain time to detect serum urea nitrogen and blood lactic acid, and a liver is taken to detect liver glycogen. As a result, after the oral administration of different dosages of theanine to a mouse for 30 days, the weight-bearing swimming time of the mouse can be obviously prolonged, the liver glycogen consumption can be reduced, the serum urea nitrogen level during exercise can be reduced, the increase of blood lactic acid of the mouse after exercise can be obviously inhibited, the elimination of the blood lactic acid after exercise can be promoted, and therefore, the theanine has the anti-fatigue effect. The mechanism of the method is probably related to that theanine can inhibit the secretion of s-hydroxytryptamine and promote the secretion of catecholamine (5-hydroxytryptamine has an inhibiting effect on the central nervous system, and catecholamine has an exciting effect).

With the wide use of probiotics, effective protection of the physiological activities of probiotics is the focus of current probiotic research. In the processing process of the probiotics, the activity of the probiotics can be influenced by the external environment such as temperature, humidity, pressure and the like, and the activity of the probiotics can be greatly lost.

For a probiotic product, maintaining a potent viability during use and storage is the most effective criterion for evaluating the product's quality. As far as today, most probiotic products have a reduced viability of live bacteria within a defined period of time, so that in most cases the probiotic bacteria tend to be less desirable. How to improve the activity of probiotics and the probiotic effect is always a focus of attention and research. The probiotic bacteria are sensitive to the external environment, which prevents the application of the probiotic bacteria in food, and the microcapsule technology can solve the problem, so that the probiotic bacteria live and colonize in the gastrointestinal tract. When the probiotic microcapsules are applied to food, the survival rate of probiotics is improved, the shelf life of the product is prolonged, and the sensory acceptability of the product is not affected. In recent years, probiotic microcapsules have emerged in some food products.

The microcapsule technology is applied to the food field, and a new way is opened up for the probiotics to resist the severe environment such as gastric acid and the like and prolong the shelf life of the product. However, the improvement of the gastric acidity resistance and the enteric solubility resistance of the embedded probiotic microcapsules is also a technical difficulty which needs to be solved urgently.

The main object of the invention is to develop a novel tablet candy which overcomes the disadvantages and has the functions of refreshing, relieving fatigue, enhancing memory and learning ability and improving work and learning efficiency.

Disclosure of Invention

The first purpose of the invention comprises providing a nutritional supplement which has the functions of refreshing, relieving fatigue, enhancing memory and learning ability, and improving work and learning efficiency.

In order to achieve the above purpose, the embodiment of the invention adopts the following technical scheme:

a nutritional supplement is provided, which comprises black tea powder, medium chain triglyceride, theanine, and inulin.

Further, the nutritional supplement also comprises composite probiotic powder.

Furthermore, the nutritional supplement comprises, by weight, 70-90 parts of black tea powder, 70-90 parts of medium-chain triglyceride, 70-90 parts of tea theanine, 800-1000 parts of inulin and 50-70 parts of composite probiotic powder.

Furthermore, the nutritional supplement comprises 80 parts of black tea powder, 80 parts of medium-chain triglyceride, 80 parts of tea theanine, 900 parts of inulin and 60 parts of composite probiotic powder by weight.

Further, the medium chain triglyceride is a beta-cyclodextrin inclusion compound of the medium chain triglyceride, and is prepared by the following steps:

adding 3-5 parts of beta-cyclodextrin into 15-25 parts of distilled water, placing the mixture in a colloid mill, grinding for 6-10min, slowly adding 1 part of medium chain triglyceride diluted by 1-3 parts of ethanol solution, grinding for 20-30min, refrigerating for 24h, performing suction filtration, pre-cooling in an ultralow temperature refrigerator to below-40 ℃, placing a sample in a freezing chamber of a freeze dryer, freezing to a temperature lower than-40 ℃, vacuumizing, and drying for 24-48 h to obtain the beta-cyclodextrin inclusion compound.

Preferably, 4 parts of beta-cyclodextrin is added with 20 parts of distilled water, the mixture is placed in a colloid mill and ground for 8min, 1 part of medium chain triglyceride diluted by 2 parts of ethanol solution is slowly added, the mixture is ground for 25min, refrigerated for 24h, and is pre-cooled to below-45 ℃ in an ultra-low temperature refrigerator after being filtered, a sample is placed in a freezing chamber of a freeze dryer and frozen until the temperature is lower than-45 ℃, and the sample is vacuumized and dried for 36 h, so that the beta-cyclodextrin inclusion compound is obtained.

Further, the nutritional supplement is a complex probioticThe bacterial powder is enteric-coated particles of mixed bacterial powder of lactobacillus plantarum Lp90, bifidobacterium longum BL21, lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium adolescentis, bifidobacterium bifidum and the like in a weight ratio. The compound probiotic enteric-coated particle is prepared by the following steps: resuspending the complex probiotic powder in physiological saline to obtain 10 of the bacterial suspension ¹⁰ CFU/mL, preparing a solution containing 2% -3% sodium alginate, 1% -2% k-carrageenan and 0.3% -0.7% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1 (4-6), adding the mixture into vegetable oil containing 0.8-1.2% of PEG-100 stearate, mechanically stirring to form water-in-oil droplets, washing the water-in-oil droplets by using 0.8-1.2% of Tween 80 aqueous solution, and then placing the water-in-oil droplets into a vacuum freeze dryer to dry the water-in-oil droplets for 36-72 hours to obtain freeze-dried primary microcapsules; putting the freeze-dried primary microcapsule into 2-3% sodium alginate and 0.8-1.2% guar gum solution, mixing, spraying the mixed solution into 1.8-2.2% CaCO ₃ And 0.01 to 0.03 percent of tween-80 mixed solution, then the mixed solution is put into a vacuum freeze dryer to be dried for 36 to 72 hours, and the freeze-dried secondary microcapsule is prepared; weighing 28-32 parts of glyceryl monostearate, heating to melt and disperse into 500-700 parts of water, adding 110-130 parts of triethyl citrate, shearing and stirring to uniformly disperse, weighing 1800-2200 parts of the Eudragit L30D-55 aqueous dispersion, stirring and mixing with the prepared liquid uniformly to serve as enteric coating liquid, and coating by adopting a fluidized bed bottom spray coating device and taking 600-1000 parts of freeze-dried secondary microcapsules as coating bed materials at the air inlet temperature of 33-37 ℃, the material temperature of 23-27 ℃ and the air outlet temperature of 20-30 ℃ to prepare the enteric particles.

Preferably, the composite probiotic bacteria powder is resuspended in physiological saline to make the bacteria suspension 10 ¹⁰ CFU/mL, 2.5% sodium alginate, 1.5% k-carrageenan and 0.5% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1; freeze-drying to the first stagePlacing the microcapsule in 2.5% sodium alginate, 1.0% guar gum solution, mixing, spraying the mixed solution at high pressure into 2.0% CaCO ₃ And 0.02 percent of tween-80 mixed solution, then placing the mixture in a vacuum freeze dryer, and drying the mixture for 54 hours to prepare a freeze-dried secondary microcapsule; weighing 30 parts of glyceryl monostearate, heating to melt and disperse the glyceryl monostearate into 600 parts of water, adding 120 parts of triethyl citrate, shearing and stirring to uniformly disperse the glyceryl monostearate, weighing 2000 parts of the Eudragit L30D-55 aqueous dispersion, uniformly stirring and mixing the aqueous dispersion with the prepared liquid to obtain an enteric coating solution, adopting a fluidized bed bottom spraying coating device, taking freeze-dried secondary microcapsules as a coating bed material, and coating at an air inlet temperature of 35 ℃, a material temperature of 25 ℃ and an air outlet temperature of 25 ℃ to prepare the enteric particles.

In order to improve the utilization rate of active ingredients of the product, enhance the function of the product and facilitate the taking and storage, the invention provides a nutritional supplement tablet and a preparation method thereof.

The nutritional supplement tablet is prepared by uniformly mixing compound probiotic enteric-coated particles, a medium-chain triglyceride beta-cyclodextrin inclusion compound, inulin, tea theanine, sorbitol and magnesium stearate and tabletting.

The third purpose of the invention also comprises the application of the nutritional supplement or the nutritional supplement tablet in preparing health-care food or special medical food.

The beneficial effects of the embodiment of the invention include:

1. the memory and the learning ability are enhanced, and the working and learning efficiency is improved;

2. has refreshing and fatigue relieving effects;

3. the survival rate of the probiotics in gastric juice is improved, and the reproductive capacity of the probiotics in intestinal tracts is enhanced, so that defecation is smooth;

4. the utilization rate of active ingredients is improved, and the occurrence of adverse reactions in vivo is reduced;

5. is convenient for administration and storage.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are conventional products which are not indicated by manufacturers and are commercially available.

Example 1

800g of black tea powder, 800g of medium-chain triglyceride, 800g of tea theanine and 9000g of inulin.

Adding 3200g of beta-cyclodextrin into 16000ml of distilled water, placing in a colloid mill, grinding for 8min, slowly adding medium chain triglyceride diluted by 1600ml of ethanol solution, grinding for 25min, refrigerating for 24h, performing suction filtration, pre-cooling to-45 ℃ in an ultralow temperature refrigerator, placing a sample in a freezing chamber of a freeze dryer, freezing to-45 ℃, vacuumizing, and drying for 36 h to obtain the beta-cyclodextrin inclusion compound.

Black tea powder, beta-cyclodextrin inclusion compound, inulin, theanine, 40g magnesium stearate and 20000g sorbitol, mixing uniformly and tabletting to obtain the product.

Example 2

The formula comprises 700g of black tea powder, 900g of medium-chain triglyceride, 700g of tea theanine, 10000g of inulin and 500g of compound probiotic powder.

Adding 2700g of beta-cyclodextrin into 13500ml of distilled water, placing in a colloid mill, grinding for 6min, slowly adding medium chain triglyceride diluted by 900ml of ethanol solution, grinding for 30min, refrigerating for 24h, performing suction filtration, pre-cooling to-50 ℃ in an ultralow temperature refrigerator, placing a sample in a freezing chamber of a freeze dryer, freezing to-40 ℃, vacuumizing, and drying for 48 h to obtain the beta-cyclodextrin inclusion compound.

Resuspending the complex probiotic powder in physiological saline to obtain 10 of the bacterial suspension ¹⁰ CFU/mL, 2% sodium alginate, 2% k-carrageenan and 0.3% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1Microcapsules; placing the lyophilized primary microcapsule in 3% sodium alginate and 0.8% guar gum solution, mixing, spraying the mixed solution at high pressure into 2.2% CaCO ₃ And 0.01 percent of tween-80 mixed solution, then placing the mixture in a vacuum freeze dryer, and drying the mixture for 72 hours to prepare a freeze-dried secondary microcapsule; weighing 20g of glyceryl monostearate, heating to melt and disperse the glyceryl monostearate into 500ml of water, adding 90g of triethyl citrate, shearing and stirring to uniformly disperse the glyceryl monostearate, weighing 1500g of the Youteqi L30D-55 aqueous dispersion, stirring and mixing the aqueous dispersion with the prepared liquid uniformly to obtain an enteric coating solution, adopting a fluidized bed bottom spraying coating device, taking the freeze-dried secondary microcapsule as a coating substrate, and coating at the air inlet temperature of 37 ℃, the material temperature of 23 ℃ and the air outlet temperature of 30 ℃ to prepare the enteric particles.

Black tea powder, a beta-cyclodextrin inclusion compound, composite probiotic enteric-coated particles, inulin, theanine, 40g of magnesium stearate and 20000g of sorbitol are uniformly mixed and tabletted to obtain the oral liquid.

Example 3

The black tea powder is 900g, the medium-chain triglyceride is 700g, the tea theanine is 900g, the inulin is 8000g, and the composite probiotic bacteria powder is 700g.

Adding 3500g of beta-cyclodextrin into 17500ml of distilled water, placing in a colloid mill, grinding for 10min, slowly adding medium chain triglyceride diluted by 2100ml of ethanol solution, grinding for 20min, refrigerating for 24h, performing suction filtration, pre-cooling to-40 ℃ in an ultralow temperature refrigerator, placing a sample in a freezing chamber of a freeze dryer, freezing to-50 ℃, vacuumizing, and drying for 24h to obtain the beta-cyclodextrin inclusion compound.

Resuspending the complex probiotic powder in physiological saline to obtain 10 of the bacterial suspension ¹⁰ CFU/mL, 3% sodium alginate, 1% k-carrageenan and 0.7% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1; placing the lyophilized primary microcapsule in 2% sodium alginate and 1.2% guar gum solution, and mixingMixing well, then spraying the mixed solution at high pressure into 1.8% CaCO ₃ And 0.03 percent of tween-80 mixed solution, then placing the mixture in a vacuum freeze dryer, and drying the mixture for 36 hours to prepare a freeze-dried secondary microcapsule; weighing 32g of glyceryl monostearate, heating to melt and disperse the glyceryl monostearate into 500ml of water, adding 130g of triethyl citrate, shearing and stirring to uniformly disperse the glyceryl monostearate, weighing 1800g of the Youteqi L30D-55 aqueous dispersion, uniformly stirring and mixing the aqueous dispersion with the prepared liquid to obtain an enteric coating solution, and coating by adopting a fluidized bed bottom spraying coating device and taking freeze-dried secondary microcapsules as a coating base material at the air inlet temperature of 33 ℃, the material temperature of 27 ℃ and the air outlet temperature of 20 ℃ to prepare enteric particles.

Black tea powder, a beta-cyclodextrin inclusion compound, composite probiotic enteric-coated particles, inulin, theanine, 40g of magnesium stearate and 20000g of sorbitol, uniformly mixing and tabletting to obtain the traditional Chinese medicine composition.

Example 4

800g of black tea powder, 800g of medium-chain triglyceride, 800g of tea theanine, 9000g of inulin and 600g of composite probiotic bacteria powder.

Adding 3200g of beta-cyclodextrin into 16000ml of distilled water, placing in a colloid mill, grinding for 8min, slowly adding medium chain triglyceride diluted by 1600ml of ethanol solution, grinding for 25min, refrigerating for 24h, performing suction filtration, pre-cooling to-45 ℃ in an ultralow temperature refrigerator, placing a sample in a freezing chamber of a freeze dryer, freezing to-45 ℃, vacuumizing, and drying for 36 h to obtain the beta-cyclodextrin inclusion compound.

Resuspending the complex probiotic powder in physiological saline to obtain 10 of the bacterial suspension ¹⁰ CFU/mL, 2.5% sodium alginate, 1.5% k-carrageenan and 0.5% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1; placing the lyophilized primary microcapsule in 2.5% sodium alginate and 1.0% guar gum solution, mixing, spraying the mixed solution at high pressure into 2.0% CaCO ₃ 0.02% Tween-80Putting the solution into a vacuum freeze dryer, and drying for 54 hours to obtain a freeze-dried secondary microcapsule; weighing 25g of glyceryl monostearate, heating to melt and disperse the glyceryl monostearate into 500ml of water, adding 96g of triethyl citrate, shearing and stirring to uniformly disperse the glyceryl monostearate, weighing 1600g of the Youteqi L30D-55 aqueous dispersion, stirring and mixing the aqueous dispersion with the prepared liquid uniformly to obtain an enteric coating solution, adopting a fluidized bed bottom spraying coating device, taking freeze-dried secondary microcapsules as a coating bed material, and coating at an air inlet temperature of 35 ℃, a material temperature of 25 ℃ and an air outlet temperature of 25 ℃ to prepare the enteric particles.

Black tea powder, a beta-cyclodextrin inclusion compound, composite probiotic enteric-coated particles, inulin, theanine, 40g of magnesium stearate and 20000g of sorbitol are uniformly mixed and tabletted to obtain the oral liquid.

Comparative example 1

The same procedure as in example 1 was repeated except that no black tea powder was added.

Comparative example 2

No medium chain triglycerides and the rest the same as in example 1.

Comparative example 3

The same procedure as in example 1 was repeated except that theanine was not contained in the tea leaves.

Comparative example 4

The complex probiotic was not embedded and the rest was the same as in example 4.

Comparative example 5

The medium chain triglycerides were not included and were otherwise identical to example 4.

Comparative example 6

The lyophilized primary microcapsules were not prepared as lyophilized secondary microcapsules and were directly compressed, except as in example 4.

Comparative example 7

The lyophilized secondary microcapsules were not prepared as enteric particles and directly tableted, except as in example 4.

Comparative example 8

The same procedure as in example 4 was repeated except that no black tea powder was added.

Comparative example 9

No medium chain triglycerides and the rest the same as in example 4.

Comparative example 10

The same procedure as in example 4 was repeated except that theanine was not present in the tea leaves.

Comparative example 11

The same procedure as in example 4 was repeated except that the lyophilized primary microcapsules and the lyophilized secondary microcapsules were not prepared, and were directly prepared as enteric-coated particles.

Comparative example 12

The lyophilized primary microcapsules were not prepared as lyophilized secondary microcapsules, but were directly prepared as enteric microparticles, and the rest were the same as in example 4.

1. Animal experiments

1. Influence on learning ability

1.1 Experimental methods

1.1.1 animal grouping, modeling and administration

Male SD rats of 3-4 months of age were selected, and the body mass (170 st 20) g was randomly divided into a normal control group, a model group, example 1-4 groups and comparative example 1-12 groups, each group consisting of 10 animals. The model group, examples 1-4 and comparative examples 1-12 were injected subcutaneously with 3% D-galactose saline solution at the back of the neck of 150 mg/kg. D for 6 weeks, and the normal control group was injected subcutaneously with the same amount of saline as the control, and the normal control group and the model group of examples 1-4 and comparative examples 1-12 were administered with the dose calculated by the standard animal equivalent dose conversion factor method (calculated as 50g per day for adults), and were administered with the same amount of starch for 6 weeks.

1.1.2 test indexes

After 6 weeks, each group of rats was subjected to a localized sailing experiment (for 5d, twice in the morning and afternoon of each day) in the Morris water maze, and the escape latency of the 5 th morning of the rats was measured, and the average of the escape latencies was calculated as the learning score. A space exploration test is carried out in the afternoon on the 5 th day, the swimming distance of the rat in a platform quadrant in 120s accounts for the percentage of the total distance, the times of passing through the position of the platform in 120s are recorded, the times of passing through the original platform are calculated in 1 min, and a route map of the rat searching the platform in 120s is recorded.

1.2 results of the experiment

TABLE 1

The details which are not described in the present invention are the common knowledge which can be selected by the ordinary skilled person in the art. Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. A nutritional supplement comprising black tea powder, medium chain triglycerides, theanine, and inulin.

2. The nutritional supplement according to claim 1, further comprising a complex probiotic powder.

3. The nutritional supplement according to claim 2, wherein the black tea powder is 70-90 parts, the medium-chain triglyceride is 70-90 parts, the theanine is 70-90 parts, the inulin is 800-1000 parts, and the composite probiotic powder is 50-70 parts by weight.

4. The nutritional supplement according to claim 2, wherein the black tea powder is 80 parts, the medium-chain triglyceride is 80 parts, the theanine is 80 parts, the inulin is 900 parts, and the composite probiotic powder is 60 parts by weight.

5. The nutritional supplement of claim 2, wherein the medium chain triglyceride is a beta-cyclodextrin inclusion of a medium chain triglyceride; the composite probiotic powder is enteric-coated particles of mixed bacterial powder of lactobacillus plantarum Lp90, bifidobacterium longum BL21, lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium adolescentis and bifidobacterium bifidum.

6. The nutritional supplement of claim 2, wherein the nutritional supplement is a tablet.

7. A nutritional supplement according to claim 5, wherein the inclusion complex of β -cyclodextrin is prepared by:

adding 3-5 parts of beta-cyclodextrin into 15-25 parts of distilled water, placing the mixture in a colloid mill, grinding for 6-10min, slowly adding 1 part of medium chain triglyceride disclosed in claim 5 diluted by 1-3 parts of ethanol solution, grinding for 20-30min, refrigerating for 24h, performing suction filtration, pre-cooling in an ultra-low temperature refrigerator to below-40 ℃, placing a sample in a freezing chamber of a freeze dryer, freezing to a temperature lower than-40 ℃, vacuumizing, and drying for 24-48 h to obtain the beta-cyclodextrin inclusion compound.

8. A nutritional supplement according to claim 5, wherein the enteric microparticles are prepared by: resuspending the composite probiotic powder in physiological saline to obtain 10 of the bacterial suspension ¹⁰ CFU/mL, preparing a solution containing 2% -3% sodium alginate, 1% -2% k-carrageenan and 0.3% -0.7% CaCl ₂ Uniformly mixing the bacterial suspension and the prepared mixed solution according to the volume ratio of 1 (4-6), adding the mixture into vegetable oil containing 0.8-1.2% of PEG-100 stearate, mechanically stirring to form water-in-oil droplets, washing the water-in-oil droplets by using 0.8-1.2% of Tween 80 aqueous solution, and then placing the water-in-oil droplets into a vacuum freeze dryer to dry the water-in-oil droplets for 36-72 hours to obtain freeze-dried primary microcapsules; putting the freeze-dried primary microcapsule into 2-3% sodium alginate and 0.8-1.2% guar gum solution, mixing, spraying the mixed solution into 1.8-2.2% CaCO ₃ And 0.01 to 0.03 percent of tween-80 mixed solution, then the mixed solution is put into a vacuum freeze dryer to be dried for 36 to 72 hours, and the freeze-dried secondary microcapsule is prepared; weighing 28-32 parts of glyceryl monostearate, heating to melt, dispersing into 500-700 parts of water, adding 110-130 parts of triethyl citrate, shearing and stirring to uniformly disperse, weighing 1800-2200 parts of the Eudragit L30D-55 aqueous dispersion, stirring and mixing with the prepared liquid uniformly to obtain an enteric coating solution, and adopting a fluidized bed bottom spray coating device to freeze-dry 700-800 parts of secondary micro-gelThe capsule is used as coating base material, and the enteric-coated particles are prepared by coating at the air inlet temperature of 33-37 ℃, the material temperature of 23-27 ℃ and the air outlet temperature of 20-30 ℃.

9. A nutritional supplement tablet according to claim 6, wherein the tablet is prepared by mixing enteric particles according to claim 8, beta-cyclodextrin inclusion compound of medium chain triglyceride according to claim 7, inulin, theanine, sorbitol and magnesium stearate, and tabletting.

10. Use of the nutritional supplement according to any one of claims 1-8 or the nutritional supplement tablet according to claim 9 for the preparation of a health food or a medical speciality food.