CN115678872A - 新型Cas13蛋白及其筛选方法和应用 - Google Patents

新型Cas13蛋白及其筛选方法和应用 Download PDF

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CN115678872A
CN115678872A CN202210632683.7A CN202210632683A CN115678872A CN 115678872 A CN115678872 A CN 115678872A CN 202210632683 A CN202210632683 A CN 202210632683A CN 115678872 A CN115678872 A CN 115678872A
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周海波
许争争
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Shanghai Jingqi Biotechnology Co.,Ltd.
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Abstract

新型Cas13蛋白及其筛选方法和应用。本公开内容涉及生物技术及医学领域。更具体地，本公开内容涉及新的Cas13家族蛋白、筛选新的Cas13家族蛋白的方法、以及相应的RNA编辑系统及其应用。本公开内容尤其涉及Cas13蛋白及相关的RNA编辑系统。所述新型Cas13蛋白的分子量较低，并且包含较多的拓展的HEPN结构域。本公开内容首次提出快速寻找具有低分子量Cas13蛋白的方法，并获得了多种新的Cas13蛋白，具有广阔的应用前景和巨大的市场价值。

Description

新型Cas13蛋白及其筛选方法和应用
相关申请
本申请要求于2021年6月4日提交的标题为“新型Cas13蛋白及其筛选方法和应用”的申请号为的申请的优先权，其全部内容通过引用并入本文。
技术领域
本公开内容涉及生物技术及医学领域。更具体地，本公开内容涉及新的Cas13家族蛋白、筛选新的Cas13家族蛋白的方法、以及相应的RNA编辑系统及其应用。本公开内容尤其涉及Cas13蛋白及相关的RNA编辑系统。
背景技术
CRISPR-Cas系统是原核生物(主要是细菌和古细菌)的一种适应性免疫系统，能够对外源病毒(例如噬菌体)的入侵进行防御。依据作用对像不同可以分为两大类，一类是靶向DNA的CRISPR-Cas系统，例如CRISPR-Cas9；另一类则是靶向RNA的CRISPR-Cas系统，例如Cas13家族，包括Cas13a、Cas13b、Cas13c、Cas13d、Cas13X、Cas13Y，等。与DNA编辑的CRISPR-Cas系统相比，CRISPR-Cas13系统主要由HEPN(higher eukaryotes and prokaryotesnucleotide)结构域来发挥切割和抵御外来入侵核酸，因而靶向RNA的CRISPR-Cas的作用更加温和且直接，能够在不改变基因组的情况下进行RNA转录本的调控，提高了基因编辑的安全性。然而当前已有发现的CRISPR-Cas系统的效应蛋白分子量都很大(>900个氨基酸)，多数都是需要多Cas蛋白组合才能发挥功能，而分子量太大不利于慢病毒和腺相关病毒(adeno-associated virus，AAV)的包装，极大了限制了CRISPR-Cas蛋白在真核生物(尤其是人源细胞)中的转染应用。因此，寻找分子量更低且特异性更强的新型CRISPR-Cas蛋白仍是本领域中的重要任务。
发明内容
针对现有筛选新型CRISPR-Cas蛋白技术的不足和实际需求，本公开内容提供了一种快速寻找分子量较低(氨基酸数目为300到950)并且包含较多的拓展的HEPN结构域(至少2个)的新型CRISPR-Cas13直系同源蛋白的方法并从生物信息分析层面(例如，序列比对、蛋白结构预测等)和实验层面验证了候选蛋白的RNA编辑活性。这些蛋白潜在应用于RNA层面的调控、编辑、检测等方面，具有广阔的学术价值和商业应用价值。
本公开内容所解决的技术问题是如何快速寻找新型的分子量较低且RNA酶切活性结构域(拓展的HEPN结构域)较多的候选CRISPR-Cas13蛋白及其系统；其次是验证候选CRISPR-Cas13蛋白及其系统的活性；并最终获得了多种新型Cas13蛋白。
本公开内容实现了以下技术效果：
(1)开发了快速筛选新型低分子量的Cas13家族蛋白的分析方法，该方法可以对新更新的原核微生物DNA序列和宏基因组序列进行CRIPSR array系统的分析和相关效应蛋白的筛选；
(2)筛选的低分子量Cas13家族成员，拓展CRISPR-Cas13的应用范围，能够更容易的被腺相关病毒等递送载体包装从而进行相关疾病诊疗，如神经相关退行性疾病的诊疗，在植物领域则可以开展育种，逆境胁迫等方面的研究，在微生物领域可以进行相关工程菌的改造等；
(3)低分子量的Cas13蛋白对于人工合成而言能够进一步节省成本；
(4)本方法在筛选过程中，除利用Cas13蛋白的已知HEPN结构域进行筛选外，还将其他种类的蛋白质中具备RNA切割活性的保守型结构域包括在内，从而提供了筛选新的Cas13蛋白的可能，并且由于这些新Cas13蛋白中这些新的功能结构域的鉴定，为进一步改造Cas13蛋白提供了新的思路和可能性。
在本公开内容的一个方面中，提供了一种鉴定新的功能性Cas13蛋白的方法，其包括：
a)CRISPR array区域的鉴定，其包括从细菌、古细菌基因组以及宏基因组序列中鉴定CRISPR array区域；
b)在所鉴定的CRISPR array区域附近搜寻Cas13候选蛋白，其包括在所鉴定区域的上游和下游检索包含目标结构域的蛋白质，所述目标结构域为HEPN结构域和其他具有RNA切割活性的结构域；
c)对检索到的蛋白质进行氨基酸(AA)长度分析，选取包含至少2个目标结构域并且长度小于950个氨基酸的Cas13候选蛋白；
d)从c)得到的结果中除去功能已知的蛋白质；以及
e)对候选蛋白进行功能验证，筛选出功能性Cas13蛋白。
在一个优选的实施方案中，所述目标结构域为氨基酸序列RX1X2X3X4H(R4xH)、RX1X2X3X4X5H(R5xH)和RX1X2X3X4X5X6H(R6xH)，其中X1、X2、X3、X4、X5、X6为任何氨基酸，其中X1优选为N、Q、H或D，其中R4xH中的X1更优选为N、D、Q、H。
在一个优选的实施方案中，所述HEPN结构域来自于表3中的序列。
在一个优选的实施方案中，所述其他具有RNA切割活性的结构域来自表2中的序列。
在一个优选的实施方案中，在步骤b)中，对所鉴定的CRISPR array区域上下游距离最近的各6个ORF进行目标结构域检索。
在一个优选的实施方案中，所述功能验证包括验证对RNA的切割活性，所述Cas13蛋白能够与所在所属物种基因组的CRISPR array上的同向重复(DR)序列和间隔区域结合形成复合物而发挥RNA切割作用
在本公开内容的另一个方面中，提供了通过上述方法筛选到的Cas13蛋白。
在一个优选的实施方案中，所述Cas13蛋白包含如SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列，或具有一个或更多个残基的保守氨基酸取代的SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列。
在一个优选的实施方案中，所述Cas13蛋白的RNA切割活性被保留。
在一个优选的实施方案中，所述Cas13蛋白的HEPN结构域或RNA切割结构域经进一步修饰或改造，而使其RNA切割活性降低或消除，成为RNA切割活性降低或消除的dCas13。
在一个优选的实施方案中，所述Cas13蛋白与一个或更多个异源功能性结构域融合。
在一个优选的实施方案中，所述融合在所述Cas13蛋白的N端、C端或者内部。
在一个优选的实施方案中，所述一个或更多个异源功能性结构域具有以下活性：脱氨酶如胞苷脱氨基酶和脱氧腺苷脱氨基酶、甲基化酶、去甲基化酶、转录激活、转录抑制、核酸酶、单链RNA裂解、双链RNA裂解、单链DNA裂解、双链DNA裂解、DNA或RNA连接酶、报告蛋白、检测蛋白、定位信号、或其任意组合。在本公开内容的另一个方面中，提供了一种核酸分子，其包含编码上述Cas13蛋白的核苷酸序列。
在一个优选的实施方案中，所述核酸分子针对在特定宿主细胞中的表达而进行了密码子优化。
在一个优选的实施方案中，所述宿主细胞是真核生物细胞，优选人细胞。
在一个优选的实施方案中，所述核酸分子包含与编码Cas13的核苷酸序列有效链接的启动子，其为组成型启动子、诱导型启动子、组织特异性启动子或发育特异性启动子。
在本公开内容的另一个方面中，提供了一种表达载体，其包含上述核酸分子。
在一个优选的实施方案中，所述表达载体为腺相关病毒(AAV)、腺病毒、慢病毒、逆转录病毒、单纯孢疹病毒、溶瘤病毒。
在本公开内容的另一个方面中，提供了一种递送系统，其包含(1)上述表达载体，或上述Cas13蛋白；以及(2)递送载体。
在一个优选的实施方案中，所述递送载体是纳米颗粒、脂质体、外泌体、微囊泡或基因枪。
在本公开内容的另一个方面中，提供了一种CRISPR-Cas系统，其包含：(1)上述Cas13蛋白或核酸分子，或者其衍生物或功能片段；(2)用于靶向目标RNA的gRNA序列。
在一个优选的实施方案中，其中所述gRNA序列包含同向重复(DR)序列和靶向靶RNA部分的间隔区域的序列。
在一个优选的实施方案中，其中所述DR序列为表4中所示序列；其中所述间隔区序列为15-60个核苷酸，优选25-50个核苷酸，更优选30个核苷酸。
在一个优选的实施方案中，所述DR序列可以是对应以下任一项的衍生物，其中所述衍生物(i)与表4中所示序列中的任一个相比，具有一个或多个(例如1、2、3、4、5、6、7、8、9或10)个核苷酸的添加、缺失、或取代；(ii)与表4中所示序列中任何一个具有至少20％、30％、40％、50％、60％、70％、80％、90％、95％或97％的序列同一性；(iii)在严格条件下与表4中所示序列任意一个，或与(i)和(ii)中的任意一个杂交；或(iv)是(i)-(iii)中任何一个的互补物，条件是所述衍生物非表4中所示序列中的任何一个，并且所述衍生物编码一个RNA，或本身即是一个RNA，所述RNA与SEQ ID NO：75-105编码的任意RNA基本保持相同的二级结构。
在一个优选的实施方案中，所述CRISPR-Cas系统还包含：(3)靶RNA。
在一个优选的实施方案中，所述CRISPR-Cas系统引起靶RNA序列的降解、切割或序列的改变。
在一个优选的实施方案中，所述靶RNA是mRNA或ncRNA，包括选自lncRNA、miRNA、misc_RNA、Mt_rRNA、Mt_tRNA、rRNA、scaRNA、scRNA、snoRNA、snRNA、sRNA的非编码RNA。
在本公开内容的另一个方面中，提供了一种细胞，其包含上述Cas13蛋白、核酸分子、表达载体、递送系统或CRISPR-Cas系统。
在一个优选的实施方案中，所述细胞为原核细胞或真核细胞，优选人细胞。
在本公开内容的另一个方面中，提供了一种降解或切割目的细胞中靶RNA、修饰目的细胞中靶RNA的序列的方法，其包括使用上述Cas13蛋白、核酸分子、表达载体、递送载体或CRISPR-Cas系统。
在一个优选的实施方案中，所述目的细胞为原核细胞或真核细胞，优选人细胞。
在一个优选的实施方案中，其中所述目的细胞为离体细胞、体外细胞或体内细胞。
附图说明
图1是从头寻找CRISPR-Cas系统低分子量蛋白流程图。
图2是体外细胞试验验证候选蛋白活性策略。
图3-11示出了随机对候选蛋白在细胞层面上的验证的结果。
图3示出了在哺乳动物细胞系中检测Cas13d[xdz9](阳性对照)酶切活性的实验结果：上图为含有cas13d蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组px261为实验组。
图4示出了在哺乳动物细胞系中检测候选蛋白DZ102酶切活性的实验结果：图4A为含有DZ102蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP487、AP489、AP491为实验组。图4B为共转染48h后进行流式分析的红光mcherry的knock down(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg4，其中sg1,sg3,sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ102潜在具有Rnase活性。
图5A示出了在哺乳动物细胞系检中测候选蛋白DZ78酶切活性实验结果：上图为含有DZ78蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP671、HP28、HP25为实验组。
图5B示出了在哺乳动物细胞系中检测候选蛋白DZ78酶切活性的实验结果：上图为含有DZ78蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系48h后进行流式分析细胞的结果，其中横坐标是指示绿色荧光；纵坐标表示红色荧光；px262为阴性对照组，AP671、HP28、HP25为实验组。
图6示出了在哺乳动物细胞系中检测候选蛋白DZ41酶切活性的实验结果：上图为含有DZ41蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP599、AP593为实验组。
图7示出了在哺乳动物细胞系检测候选蛋白DZ77酶切活性实验结果：图7A为含有DZ77蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP659为实验组。图7B则表示共转染48h后进行流式分析的红光mcherry的knockdown(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg5，其中sg2，sg3，sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ77潜在具有Rnase活性。
图8示出了在哺乳动物细胞系中检测候选蛋白DZ79酶切活性的实验结果：上图为含有DZ79蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP677为实验组。
图9示出了在哺乳动物细胞系中检测候选蛋白DZ81酶切活性的实验结果：图9A为含有DZ81蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP695为实验组。图9B则表示共转染48h后进行流式分析的红光mcherry的knock down(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg5，其中sg1，sg3，sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ81潜在具有Rnase活性。
图10示出了在哺乳动物细胞系中检测候选蛋白DZ82酶切活性的实验结果：上图为含有DZ82蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP699、AP701为实验组。
图11展示了候选蛋白在293T细胞系上验证其Knock down mCherry的验证实验，其中图11A示出了在哺乳动物细胞系中检测候选蛋白DZ75、DZ76、DZ87、DZ89、DZ95、DZ103和DZ104的酶切活性的实验结果(实验组)：上图为含有DZ75、DZ76、DZ87、DZ89、DZ95、DZ103和DZ104蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图。图11B显示了候选蛋白DZ87，DZ89，DZ95，DZ92，DZ97，DZ99，DZ101切割mCherry的流式分析结果，可以看到候选蛋白在与靶向mCherry的不同位置的sgRNA质粒共转染293T细胞系后48h的流式分析检测均表明它们具有一定程度的Rnase活性，效率不是很高，这可能与候选蛋白的潜在PFS有关。
图12示出了在哺乳动物细胞系中检测候选蛋白DZ89、DZ95和cas13d酶切活性的实验结果(对照组)：上图阴性对照为只含有DZ89、DZ95以及cas13d蛋白的质粒(不含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。而阳性对照为cas13d蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中whitelight表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图。
图13展示的是候选蛋白DZ77，DZ78，DZ81，DZ87，DZ89，DZ92，DZ95，DZ97，DZ99，DZ101，DZ102等蛋白的knock down mcherry红光的流式分析结果，从mCherry上随机筛选的3个sgRNA靶向spacer来看，候选蛋白DZ78的KD效果最好，其他蛋白则可能与其强PFS有关。
图14示出了DZ78候选蛋白切割内源基因的实验结果。图14A示出利用DZ78候选蛋白切割内源基因Ptbp1的切割效果，其中hP4、hP5、hP6为实验组不同sgRNA靶向内源基因Ptbp1的不同区域，而hP19则为阴性对照。可以发现与阴性对照相比，候选蛋白DZ78能够切割内源基因Ptbp1，而且hP4效果最好。图14B示出利用DZ78候选蛋白切割内源基因Rest的切割效果，其中hRT1、hRT2、hRT3为实验组不同sgRNA靶向内源基因Rest的不同区域，而hRT19则为阴性对照。可以发现与阴性对照相比，候选蛋白DZ78能够很好的切割内源基因Rest，而且hRT1效果最好。图14C则展示候选蛋白DZ78切割其他内源基因的knock down结果，涉及多个内源基因，包括EZH2，HRAS，EGFR，KRAS，NF2，NFKB1，NRAS，PPARG，RAF1，SMARCA4，STAT3。这些结果表明DZ78具有广泛切割RNA的能力。
图15示出了候选蛋白Direct repeat序列的RNA二级结构。图中展示的是候选蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82、DZ85和DZ102蛋白的DR二级结构。
图16示出了Cas蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102的拓展的HEPN位置模式图。
图17示出了候选蛋白DZ41、DZ77、DZ78、DZ79、DZ80、DZ82和DZ102的基因组模式图。
图18A展示候选蛋白DZ78的潜在PFS的验证实验：通过针对spacer前后设计4个连续相同碱基的sgRNA，然后进行靶向mCherry进行分别与候选蛋白共转染，转染48h后进行流式分选检测，可以发现候选蛋白DZ78在连续4碱基模式下，并没有显示很强的PFS偏好性，提示我们的候选蛋白DZ78潜在能靶向RNA更加任意的区域，具有更广泛的应用价值。
图18B展示了候选蛋白DZ78的sgRNA成熟的机制：为了验证候选蛋白引导RNA的成熟机制，我们设计4种sgRNA：包括DR-sg-DR(即为double DR)，DR-sg(即为5’方向DR)，sg-DR(即为3’方向DR)以及非靶向目标RNA的control sgRNA。同时为了验证其在细胞核内和细胞核外的Rnase活性，还对sgRNA进行添加入核信号(NLS)。从结果可以发现，不论是细胞核内还是细胞核外，候选蛋白DZ78只有在于双DR或者3’方向DR共转染时才能发挥其Rnase活性，表明候选蛋白DZ78的guide RNA成熟体系为3’方向。
图18C和图18D展示了候选蛋白DZ78的guide RNA长度对其Rnase活性的影响。通过设计共转染质粒(如图18C所示)，可以看到不同spacer长度对giude RNA引导候选蛋白DZ78靶向切割mCherry的影响很大(如图18D所示)，其中当spacer长度低于25bp的时候效率降低很多，而30bp～50bp间差异不是很大。
具体实施方式
下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于举例说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。
如在说明书中所使用的，没有数量词修饰的名词可意指一个/种或更多个/种。如在权利要求书中所使用的，当与词语“包含/包括”结合使用时，没有数量词修饰的名词可意指一个/种或多于一个/种。
权利要求书中术语“或/或者”的使用用于意指“和/或”，除非明确地指出仅指替代方案或替代方案是相互排斥的，尽管本公开内容支持仅指替代方案和“和/或”的限定。如本文中使用的“另一/另一些”可意指至少第二或更多个/种。
在整个本申请中，术语“约”用于表示值包括装置的误差、用于确定该值的方法的固有变化，或者存在于研究对象之间的固有变化。这样的固有变异可以是标注值的±10％的变异。
在整个申请中，除非另有说明，否则核苷酸序列以5’至3’方向列出，并且氨基酸序列以N端至C端方向列出。
通过以下详细描述，本发明的其他目的、特征和优点将变得明显。然而，应理解，尽管表明了本发明的一些优选实施方案，但是详细描述和具体实施例仅以举例说明的方式给出，因为根据该详细描述，在本发明的精神和范围内的多种变化和修改对于本领域技术人员而言将变得明显。
定义
NCBI(https://www.ncbi.nlm.nih.gov/)是指美国国家生物信息中心，是一个面向全世界的公共数据库，本领域技术人员利用该数据库提供的核酸数据库进行下载原核生物的基因组，蛋白质组相关数据库等，也可以利用该数据提供的blast比对软件进行序列比对的分析。
IMG(https://img.jgi.doe.gov/)是指微生物基因组整合数据库，是新一代基因组数据库的代表，不仅能够完整收录现有数据库的内容，还提供了更完善的数据上传、注释和分析服务，将测序数据储存到IMG/M数据库。该数据可以下载纯培养细菌测序基因组、宏基因组、宏基因组组装基因组、单细胞测序基因组的数据。
CRISPR(cluster regularly interspaced short palindromic repeats)是原核生物，主要是指细菌和古细菌体内的一串DNA序列，包括DR区域和spacer区域。而CRIPSR系统除了包含CRISPR array外，还包括相关的Cas蛋白。它们一起构成了细菌低于外来病毒入侵的免疫系统。
Cas13家族是目前已知该能够靶向RNA的CRIPSR酶家族，其成员包括Cas13a、Cas13b、Cas13c、Cas13d、Cas13X和Cas13Y家族。与CRISPR/Cas9切割DNA的活性不同，CRISPR/Cas13能够用于切割细菌细胞中特定的RNA序列。
HEPN结构域是higher eukaryotes and prokaryotes nucleotide domain的简称，是CRISPR-Cas13酶系统中Cas13蛋白发挥切割和抵御外来入侵核酸的重要结构域。
ABE系统是Adenine base editors的简称，即嘌呤碱基转换技术，能够实现A/T到G/C的单碱基改变。最常用的酶是adar酶(adenosine deaminases acting on RNA，一种作用于RNA的腺苷脱氨酶)。主要是通过将腺嘌呤脱氨基成肌苷，在DNA或者RNA中进行读码的时候会被看成G，从而实现A/T到G/C的突变。由于细胞对肌苷的切出修复不敏感，因而这种突变可以维持较高的产物纯度。
CBE系统是Cytidine base editor的简称，即嘧啶碱基转换技术，目前有BE1、BE2和BE3个工具，其中BE3的效率最高，因而在基因治疗，动物模型制作以及功能基因筛选等领域被广泛应用。
真核细胞例如哺乳动物细胞，包括人类细胞(人类原代细胞或已建立的人类细胞系)。所述细胞可以是非人类哺乳动物细胞，例如来自非人类灵长类动物(例如猴子)、奶牛/公牛/家牛、绵羊、山羊、猪、马、狗、猫、啮齿动物(例如兔子、小、大鼠、仓鼠)等。所述细胞来自鱼(例如鲑鱼)、鸟(例如禽鸟，包括小鸡、鸭、鹅)、爬行动物、贝类(例如牡蛎、蛤、龙虾、虾)、昆虫、蠕虫、酵母等。所述细胞可以来自植物，例如单子叶植物或双子叶植物。所述植物可以是粮食作物，例如大麦、木薯、棉花、花生、玉米、小米、油棕果、土豆、豆类、油菜籽或低芥酸菜子、大米、黑麦、高粱、大豆、甘蔗、糖甜菜、向日葵和小麦。所述植物可以是谷物(例如大麦、玉米、小米、大米、黑麦、高粱和小麦)。所述植物可以是块茎(例如木薯和土豆)。在一些实施方案中，所述植物可以是糖料作物(例如甜菜和甘蔗)。所述植物可以是含油作物(例如大豆、花生、油菜籽或低芥酸菜子、向日葵和油棕果)。所述植物可以是纤维作物(例如棉花)。所述植物可以是树木，例如桃树或油桃树、苹果树、梨树、杏树、核桃树、开心果树、柑橘属树(例如橙子、葡萄柚或柠檬树)、草、蔬菜、水果或藻类。所述植物可以是茄属植物；芸苔属(Brassica)植物；莴苣属(Lactuca)植物；菠菜属(Spinacia)植物；辣椒属(Capsicum)植物；棉花、烟草、芦笋、胡萝卜、卷心菜、西兰花、花椰菜、番茄、茄子、胡椒、生菜、菠菜、草莓、蓝莓、覆盆子、黑莓、葡萄、咖啡、可可等。
CRISPR系统
CRISPR(成簇规律间隔短回文重复序列)/Cas13(CRISPR相关蛋白13)介导的RNA编辑正在成为用于疾病诊疗、植物育种等方面的有前景的工具。
CRISPR是包含碱基序列的短重复的DNA基因座。每个重复之后是来自先前暴露于病毒的“间隔区DNA”的短区段。在约40％的测序的真细菌基因组和90％的测序的古细菌中发现CRISPR。CRISPR通常与编码与CRISPR相关的蛋白质的Cas基因相关。CRISPR/Cas系统是原核免疫系统，其赋予对外来遗传元件(例如质粒和噬菌体)的抗性并提供获得性免疫的形式。CRISPR间隔区识别并沉默真核生物体中的这些外源遗传元件(例如RNAi)。
CRISPR重复序列的大小为24至48个碱基对。它们通常显示一些二重对称，这意味着形成二级结构例如发夹，但不是真正的回文结构。重复序列被相似长度的间隔区分开。一些CRISPR间隔区序列与来自质粒和噬菌体的序列准确地匹配，尽管一些间隔区与原核生物的基因组匹配。响应于噬菌体感染，可迅速添加新的间隔区。
指导RNA(gRNA)。作为RNA指导的蛋白，Cas13需要短RNA以指导RNA靶标的识别。
核酸酶
Cas核酸酶。CRISPR相关(Cas)基因通常与CRISPR重复-间隔区阵列相关。截至2013年，已描述了超过四十个不同的Cas蛋白家族。在这些蛋白家族之中，Cas1看来在不同的CRISPR/Cas系统中是普遍存在的。Cas基因和重复序列结构的特定组合已用于限定8种CRISPR亚型(Ecoli、Ypest、Nmeni、Dvulg、Tneap、Hmari、Apern和Mtube)，其中一些与编码重复序列相关神秘蛋白(repeat-associated mysterious protein，RAMP)的另外的基因模块相关。在单个基因组中可存在多于一种CRISPR亚型。CRISPR/Cas亚型的散发性分布(sporadic distribution)表明该系统在微生物进化期间经历水平基因转移。
外源DNA明显地由Cas基因编码的蛋白质加工成小元件(长度为约30个碱基对)，然后以某种方式将其插入到靠近前导序列的CRISPR基因座中。来自CRISPR基因座的RNA是组成型表达的，并且被Cas蛋白加工成由具有侧翼重复序列的单独外源来源序列元件构成的小RNA。RNA指导其他Cas蛋白在RNA或DNA水平上沉默外源遗传元件。证据表明CRISPR亚型之间的功能多样性。Cse(Cas亚型Ecoli)蛋白(在大肠杆菌(E.coli)中称为CasA-E)形成功能性复合体Cascade，其将CRISPR RNA转录物加工成保留Cascade的间隔区-重复序列单元。在另一些原核生物中，Cas6加工CRISPR转录物。有趣的是，大肠杆菌中基于CRISPR的噬菌体灭活需要Cascade和Cas3，但不需要Cas1和Cas2。在激烈火球菌(Pyrococcus furiosus)和另一些原核生物中发现的Cmr(Cas RAMP模块)蛋白与小的CRISPR RNA形成功能性复合体，其识别和切割互补靶RNA。RNA指导的CRISPR酶被分类为V型限制酶。
实施例
实施例1：新型Cas13蛋白从头筛选
除了利用已知Cas13家族其他成员寻找其他直系同源Cas蛋白外，我们还进行了从头寻找CRISPR-Cas13其他家族成员，技术路线如图1所示。
简单来说，该分析系统包括2大块，一部分CRISPR array区域的鉴定，我们首先下载NCBI和IMG截止到2021年2月份的全部细菌，古细菌基因组以及宏基因组的序列，利用CRISPR array鉴定软件(如Pilercr)进行鉴定CRISPR array区域；另一部分是该区域上下游附近Cas相关蛋白的搜寻，即取该区域上下游临近的6个蛋白，共计12个蛋白进行目标结构域分析和氨基酸(amino acid,AA)长度分析(300到950AA)。唯有同时满足二者，且氨基酸个数更小的单个Cas效应蛋白才是候选新型小分子量的Cas潜在蛋白。最终候选蛋白的氨基酸序列、早期HEPN结构域、拓展的HEPN结构域和坐标等信息参见表1。
其中本筛选体系的HEPN结构域除了包含过去Cas13家族成员中发现的RxxxxH(R4xH)特征(我们记为早期HEPN结构域)外，还进行了拓展，包括其他具有RNA切割活性的结构域RxxxxxH(R5xH)和RxxxxxxH(R6xH)(我们将RxxxxxH(R5xH)和RxxxxxxH(R6xH)以及RxxxxH(R4xH)总体记为拓展的HEPN结构域)。其中临近R保守氨基酸优选为N、Q、H或D，例如R[NDQH]xxxH、R[NDQH]xxxxH、R[NDQH]xxxxxH等组合；其中R4xH优选R[NDQH]xxxH，而R5xH和R6xH则优选R[NDQH]xxxxH和R[NQDH]xxxxxH；其中x代表任意氨基酸，而中括号内N、D、Q和H为优先考虑保守氨基酸。
实施例2：新型低分子量候选Cas13蛋白的功能验证
候选蛋白筛到后将进行功能验证，我们采用图2所示实验技术路线图来进行操作。简而言之，我们首先将候选蛋白的核酸序列，DR序列以及target spacer序列送公司进行合成，然后将其导入表达质粒中构建相应的质粒。然后通过质粒转化在DH5a大肠杆菌感受态细胞进行质粒扩增培养，然后抽提质粒后进行人源293T(能表达红光)细胞系的转染试验，与此同时我们还设计了对应的阴性和阳性对照组来进一步确认候选蛋白的切割活性。共转染质粒48h后进行流式细胞分析等试验来最终确定候选蛋白的RNA酶切活性。
按照上述研究策略，我们随机选择了部分蛋白进行了验证。
如图3至11中所示，从DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102以及Cas13d的结果表明，我们候选的具有Rnase活性的Cas13蛋白在瞬转哺乳动物细胞系能够很好的敲低mCherry(红光)蛋白，而阴性对照组(图12)则不能切割，表现为红色荧光高亮；表明我们的候选蛋白可以在哺乳动物等上发挥Rnase的作用。而DZ87、DZ89、DZ95则显示比较弱的Rnase活性(如图11B和图13)，此外，从DZ75、DZ76、DZ103和DZ104的结果表明，这些Cas13蛋白在瞬转哺乳动物细胞系后不能有效敲低mCherry(红光)蛋白，在哺乳动物等上无法发挥Rnase的作用但是它们都有发挥Rnase活性的HEPN结构域，造成这种结果可能与该类候选蛋白具有很强的PFS偏好性，与我们随机选择的靶向mCherry的位置有关。类似地，为了验证候选蛋白广泛的适用于切割内源基因的多种RNA，我们开展了验证它们切割内源基因的能力。图14中则示出了利用DZ78候选蛋白能有效切割内源基因，如Ptbp1，Rest，EGFR，NF2等基因。表明其具有靶向不同RNA的广泛的切割效果。
实施例3：新型低分子量候选Cas13蛋白DZ78的特征鉴定
为了进一步研究候选蛋白DZ78的发挥Rnase活性的特性，我们从其靶向的偏好性(PFS)，向导RNA成熟机制以及靶向RNA的spacer长度等方向展开探索(如图18所示)：
为了探索DZ78的靶向RNA的位置偏好性，我们通过在mcherry末尾设计一段供sgRNA靶向切割且这个target位置前后各有4个连续相同的碱基的mCherry表达质粒，然后通过共转染靶向不同组合的mCherry表达质粒48h后进行流式分析，可以发现(如图18A所示)DZ78在靶向mcherry的target位置时并没有展示很强的碱基序列偏好性，提示DZ78蛋白在靶向RNA时的位置可能更加广泛，具有更加广泛的应用价值。
为了探索候选蛋白DZ78的guide RNA的成熟机制，通过设计不同4种sgRNA：包括DR-sg-DR(即为double DR)，DR-sg(即为5’方向DR)，sg-DR(即为3’方向DR)以及非靶向目标RNA的control sgRNA。同时为了验证其在细胞核内和细胞核外的Rnase活性，还对sgRNA进行添加入核信号(NLS)。从结果可以发现(如图18B所示)，不论是细胞核内还是细胞核外，候选蛋白DZ78只有在于双DR或者3’方向DR共转染时才能发挥其Rnase活性，表明候选蛋白DZ78的guide RNA成熟体系为3’方向。
为了探索候选蛋白靶向RNA的spacer长度对其发挥Rnase活性效率的影响，通过设计不同spacer长度的guide RNA共转染质粒(如图18C所示)，可以看到不同spacer长度对giude RNA引导候选蛋白DZ78靶向切割mCherry的影响很大(如图18D所示)，其中当spacer长度低于25bp的时候效率降低很多，而30bp～50bp间差异不是很大。表明候选蛋白DZ78在靶向RNA的时候其spacer长度会影响其切割RNA的效率。
实施例4：新型低分子量候选Cas13蛋白的碱基编辑功能验证
当前用于单碱基编辑的系统主要有两种，一种是ABE系统，另一种是CBE系统。简单来说，通过候选Cas13蛋白的切割结构域(拓展的HEPN结构域)进行突变处理，获得只有结合RNA而没有切割活性的候选dCas13蛋白，然后融合adar酶序列，构建ABE单碱基编辑系统的质粒，然后对特定序列，比如TP53基因的转录本进行定点碱基突变处理的sgRNA设计并构建相应的质粒载体。然后通过共转染人源293T细胞系，48小时后进行流式细胞分选获得共转染的细胞系。然后进行RNA转录本的提取以及建库。然后进deep seq测序。测序结束后通过生物信息方法分析TP53基因转录本的突变情况就可以获得对应的ABE系统的单碱基编辑效能分析。从而通过不断的优化sgRNA来实现构建目标区域的最优单碱基编辑系统。
实施例5：候选Cas13蛋白与已知Cas13蛋白的同源性分析
依据未知蛋白在已知蛋白的覆盖度越高且相似度占比越大则未知蛋白与已知蛋白的同源性越近的原理进行。对所筛选到的候选蛋白后，我们先从NCBI数据库以及专利文献中下载Cas13a,Cas13b,Cas13c，Cas13d，Cas13X，Cas13Y等的相关蛋白序列，然后与我们的数据一起合并构建本地blastp的索引文件，然后将候选蛋白序列比对到本地blastp索引库中进行蛋白序列比对分析。对于蛋白之间相似度(identity)小于20％或者没法比对到本地索引库的部分我们统一标注为20％；类似的，对于覆盖度(coverage)小于5％或者没法比对到本地索引库的标记为1％。从众多候选Cas13蛋白中选取与已知Cas13蛋白同源性低的新Cas13蛋白。随机筛选靶向mCherry蛋白kncok down实验具有Rnase活性的候选Cas13蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102的同源性分析结果参见表5。从表5可以看出，本发明方法所鉴定出的新Cas13蛋白与已知各家族Cas13蛋白的同源性水平极低。
候选蛋白信息参见下表1。
筛选所用的RNA酶和相应保守型结构域的序列信息汇总参见下表2和表3。
具有Rnase活性的候选Cas13蛋白的DR序列信息参见下表4。
具有Rnase活性的候选Cas13蛋白的同源性分析结果参见下表5。
候选Cas蛋白验证实验所用引物和spacer序列参见下表6。
Figure BDA0003679444160000151
Figure BDA0003679444160000161
Figure BDA0003679444160000171
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Figure BDA0003679444160000451
Figure BDA0003679444160000461
Figure BDA0003679444160000471
Figure BDA0003679444160000481
序列表
<110> 中国科学院脑科学与智能技术卓越创新中心
<120> 新型Cas13蛋白及其筛选方法和应用
<150> 202110625339.0
<151> 2021-06-04
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Phe Val Leu Leu Thr Gln Leu Met Leu Arg Gly Val Thr Leu Glu Lys
645 650 655
Glu Lys Lys Lys Lys Asp Lys Asp Lys Leu Asn Ala Leu Asp Thr Asp
660 665 670
Thr Val Leu Tyr Glu Glu Glu Lys Lys Lys Leu Glu Lys Gln Ile Lys
675 680 685
Pro Glu Arg Ser Leu Lys Asp Leu Val Glu Lys Gly Leu Ile Phe Leu
690 695 700
Ala Leu Asp Gln Leu Ser Ser Ser Asp Lys Asp Leu Lys Ala Ile His
705 710 715 720
Ser Glu Ile Glu Asp Met Phe Val Gly Val Ser Pro Asp Ser Asp Asn
725 730 735
Arg Asn Leu Arg Asn Arg Leu Ala His Phe Lys Asp Leu Gly Asn Lys
740 745 750
Asn Leu Ile Asn Ile Thr Ser Gln Ile Asn Glu Val Arg Lys Met Met
755 760 765
Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys Ser Met Ile Asp
770 775 780
Leu Phe Glu Arg Tyr Asn Leu Ile Leu Ser Phe Lys Val Gln Ser His
785 790 795 800
Lys Leu Gln Leu Lys Asn Leu Lys Ser Lys Gln Ile Thr His Leu Asn
805 810 815
Asn Lys Gly Ile Thr Glu Asn Leu Leu Ser Asp Asp Tyr Val Ser Val
820 825 830
Ile Lys Arg Leu Leu Leu Thr Glu Gln Asn Pro Glu
835 840
<210> 4
<211> 512
<212> PRT
<213> 宏基因组(metagenomic)
<400> 4
Met Ala Asn Arg Leu Ile Leu Ala Val Leu Val Leu Val Val Leu Gly
1 5 10 15
Ala Leu Tyr Ala Ala Ala Gly Leu Asn Arg Pro Ile Thr Leu Thr Ser
20 25 30
Ala Ala Ala Ala Gly Ala Pro Gly Gln Leu Pro Val Thr Ser Ala Leu
35 40 45
Leu Ala Cys Pro Gly Pro Gly Ser Asp Gly Pro Thr Gly Gly Gly Leu
50 55 60
Ala Ile Ala Ser Ala Pro Ala Thr Ala Ser Thr Gly Gln Val Leu Leu
65 70 75 80
Thr Gly Leu Asn Pro Ala Gly Gly Thr Gly Ser Gly Asp Gly Ala Gly
85 90 95
Thr Thr Leu His Ile Leu Thr Gln Pro Gly Val Leu Thr Leu Ala Arg
100 105 110
Val Pro Lys Ala Pro Pro Val Pro Lys Asn Leu Val Ala Ala Gln Asn
115 120 125
Met Ala Gly Gly Leu Val Pro Thr Ser Thr Ile Arg Gly Gly Val Val
130 135 140
Ile Gln Ala Val Gly Ala Met Ala Gln Gly Leu Asp Ala Glu Gln Leu
145 150 155 160
Asn Pro Ala Gly Gln Pro Thr Ala Arg Cys Gly Ala Pro Gly Ser Asp
165 170 175
Phe Trp Phe Val Ser Pro Val Thr Leu Thr Leu His Ile Glu Leu Tyr
180 185 190
Leu Leu Asn Ala Asp Asn Val Pro Ala Ser Ala Asp Val Ser Val Gln
195 200 205
Thr Asp Ser Gly Pro Leu Leu Gly Ser Pro Asp Ser Gly Ile Val Val
210 215 220
Pro Pro His Ser Leu Val Ala Gln Ser Leu Asp Lys Leu Val His Ala
225 230 235 240
Ala Arg Ala Val Ser Leu His Val Thr Thr Ser Val Gly Arg Val Val
245 250 255
Ala Ala Val Arg Glu Thr Asn Ser Lys Thr Gln Pro Gly Ser Trp Leu
260 265 270
Pro Ala Ala Gln Pro Ala Ala Thr His Gln Val Leu Ala Gly Leu Pro
275 280 285
Gly Val Pro Gly Thr Pro Glu Leu Tyr Val Ala Val Pro Gly Asn Ala
290 295 300
Ala Ala Leu Val Lys Val Thr Ala Val Thr Pro Arg Gly Ser Tyr Gln
305 310 315 320
Pro Thr Gly Gly Asn Gly Ile Ser Leu Leu Gly His Leu Thr Thr Gly
325 330 335
Ile Pro Ile Pro Ser Leu Gly Gly Ile Pro Ala Ser Ile Ser Val Ser
340 345 350
Ala Asn Val Pro Val Thr Ala Val Leu Glu Ile Pro Gly Gly Pro Ala
355 360 365
Gly Ala Leu Gly Ala Val Ile Ser Gly Ser Asp Pro Ile Gln Glu Gln
370 375 380
Gly Val Val Ala Ala Ser Pro Val Gly His Gly Val Ala Thr Glu Leu
385 390 395 400
Val Leu Ser Ala Pro Gly Gln Ala Ala Ser Val Arg Ile Ala Gln Ala
405 410 415
Leu Pro Gly Ala Val Leu Thr Gly Gln Pro Gly Gln Val Val Gln Val
420 425 430
Pro Ala Arg Ser Thr Val Val Val His Val Thr Leu Pro Arg Arg Ser
435 440 445
Ala Ser Ala Ser Gln Val Ala Leu Val Val Thr Pro Leu Pro Lys Ser
450 455 460
Gly Pro Val Tyr Ala Ala Arg Ala Ala Phe Ser Gly Gly Thr Val Gln
465 470 475 480
Thr Ile Leu Pro Val Thr Thr Ala Pro Thr Gln Ile Ser Leu Pro Asp
485 490 495
Ala Arg Glu Ser Leu Ile Thr Ile Leu Gly Leu Ser Ser Ala Asp Arg
500 505 510
<210> 5
<211> 400
<212> PRT
<213> 宏基因组(metagenomic)
<400> 5
Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Asp Arg Pro Val Ser Ala
1 5 10 15
Gly Thr Gln Glu Ile Ile Ala Ser Met Glu Pro Arg Arg Ile Arg Arg
20 25 30
Gly Asn Ser Trp Ala Gly Leu Ser Asn Arg Lys Thr Cys Lys Arg Leu
35 40 45
Gln Trp Ser Arg Gly Glu Phe Ala Ala Glu Thr Cys Arg Ser Ala Arg
50 55 60
Gly Arg Thr Pro Pro Pro Ala Cys Phe Asn Gly Ala Ala Ala Asn Ser
65 70 75 80
Pro Arg Lys Arg Glu Gly Cys Arg Gly Gly Gln Gly His Arg Pro Ala
85 90 95
Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Ala Glu Leu Leu
100 105 110
Pro Pro Lys Gly Glu Leu Leu Gly Leu Gln Trp Ser Arg Gly Glu Phe
115 120 125
Ala Ala Glu Thr Cys Arg Arg Gly Gly Leu Trp Leu Ser Ala Gly Ala
130 135 140
Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Ser Leu Cys
145 150 155 160
Ser Trp Leu Arg Leu Arg Ser Ala Gly Phe Asn Gly Ala Ala Ala Asn
165 170 175
Ser Pro Arg Lys Arg Glu Cys Ala His Pro Glu His Thr Gly Met Trp
180 185 190
Val Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Gly Glu Arg
195 200 205
Trp Pro Val Leu Asp Asp Asp Asp Glu Ala Ser Met Glu Pro Arg Arg
210 215 220
Ile Arg Arg Gly Asn Arg Ala Val Arg Gly Arg Lys Arg Glu Ala Ala
225 230 235 240
Pro Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Arg Ala
245 250 255
Gln Pro Asn Pro Glu Lys Arg His Glu Arg Phe Asn Gly Ala Ala Ala
260 265 270
Asn Ser Pro Arg Lys Arg Ser Ala Arg Gly Leu Arg Ala Asp His Pro
275 280 285
Val Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Arg Lys Asp
290 295 300
Asp Asp Cys Val Arg Ser Asp Pro Asp Ser Phe Asn Gly Ala Ala Ala
305 310 315 320
Asn Ser Pro Arg Lys Pro Arg Ile Ala Arg Gln Arg Gln Leu Met Glu
325 330 335
Ser Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Pro Gly
340 345 350
Ala Ala Leu Pro Arg Leu Gln Arg Arg Arg Phe Asn Gly Ala Ala Ala
355 360 365
Asn Ser Pro Arg Lys Pro Arg Pro Gly Ala Arg His Asp Arg Asp Ser
370 375 380
Gly Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Ala Arg Ser
385 390 395 400
<210> 6
<211> 562
<212> PRT
<213> 宏基因组(metagenomic)
<400> 6
Met Glu Thr Ala Gln Lys Val Asn Asn Val Gly Lys Arg Ile Arg Tyr
1 5 10 15
Asn Pro Phe Lys Thr Val Asp Lys His Tyr Phe Gly Gly Phe Phe Asn
20 25 30
Leu Ala Glu Asn Asn Ile Gln Glu Val Phe Glu Glu Ile Asn Leu Arg
35 40 45
Leu Lys Asn Lys Asn Ala Pro Ile Lys Asn Ile Ile Glu Lys Tyr Thr
50 55 60
Asp Glu Asn Ile Ser Leu Val Glu Tyr Glu Lys Phe Ile Ala Ile Leu
65 70 75 80
Ser Glu Tyr Phe Pro Ile Ala Lys Glu Ile Asp Gln Lys Asn Lys Lys
85 90 95
Thr Phe Asn Asp Lys Ile Ile Glu Lys Thr Arg Ile Glu Arg Val Ser
100 105 110
Asp Phe Arg Lys Asp Phe Phe Leu Phe Ile Lys Ala Ile Glu Lys Leu
115 120 125
Arg Ser Tyr Tyr Thr His Phe Lys His Asp Asp Ile Ile Ile Asp Asn
130 135 140
Gln Leu Phe Asn Leu Leu Asp Asn Ile Leu Leu Asn Thr Val Leu Glu
145 150 155 160
Thr Lys Lys Lys Tyr Leu Lys Thr Asp Lys Thr Lys Glu Leu Leu Lys
165 170 175
Asp Ser Leu Gln Glu Glu Leu Glu Lys Leu Tyr Glu Leu Lys Ile Asn
180 185 190
Gln Leu Glu Glu Lys Lys Arg Glu Val Asn Ala Leu Ile Ala Glu Gln
195 200 205
Lys Lys Asn Gly Glu Glu Thr Gln Arg Pro Phe Arg Tyr Ser Thr Asp
210 215 220
Lys Asp Gln Ile Ile Asn Ser Ile Tyr Asn Asp Ala Ile Lys Pro Phe
225 230 235 240
Leu Tyr Glu Asn Ala Asn Lys Val Glu Ile Ser Asn Lys Lys Lys Thr
245 250 255
Ala Phe Asn Glu Lys Asp Glu Leu Val Lys Glu Gly Asp Phe Asn Leu
260 265 270
Pro Ile Ser Ser Ser Gly Ile Val Phe Leu Leu Ser Cys Phe Leu Asn
275 280 285
Arg Lys Glu Ile Glu Asp Leu Lys Ala Asn Ile Lys Gly Tyr Lys Gly
290 295 300
Lys Val Ile Lys Gly Asp Val Leu Asp Leu Glu Lys Asn Ser Ile Arg
305 310 315 320
Phe Met Ala Thr His Arg Ile Tyr Ser Val Met Cys Tyr Lys Gly Leu
325 330 335
Lys Asn Lys Ile Arg Thr Ser Gln Ser Thr Thr Lys Glu Thr Leu Leu
340 345 350
Met Gln Met Ile Asp Glu Leu Ser Lys Ile Leu Asp Val Leu Tyr Lys
355 360 365
Asn Ile Ser Glu Asp Leu Gln Asn Thr Phe Thr Glu Asp Trp Asn Glu
370 375 380
Tyr Tyr Lys Asp Asn Phe Glu Asn Leu Asp Asn Leu Glu Asn Ser Lys
385 390 395 400
Val Ile His Pro Val Ile Arg Lys Arg Tyr Glu Asp Lys Phe Asn Tyr
405 410 415
Phe Ala Ile Arg Phe Leu Asp Glu Phe Val Asp Phe Pro Ser Leu Arg
420 425 430
Phe Gln Val His Leu Gly Asn Tyr Ile Lys His Ser Met Ser Lys Asn
435 440 445
Val Gly Asn Val Ile Thr Thr Arg Glu Ile Lys Asn Lys Ile Phe Val
450 455 460
Phe Gly Arg Leu Asp Glu Ile Asn Gln Tyr Lys Ser Asp Phe Phe Asn
465 470 475 480
Lys Ile Lys Glu Asn Glu Glu Glu Thr Asn Trp Glu Leu Phe Pro Asn
485 490 495
Pro Asn Tyr His Phe Pro Met Glu Asn Ser Asp Glu Leu Lys Tyr Ala
500 505 510
Asn Lys Ile Gly Ile Tyr Leu Asp Leu Trp Lys Ser Lys Glu Ile Ile
515 520 525
Asn Asp Glu Ile Glu Asn Lys Asn Lys Gln Thr Ser Ser Tyr Lys Lys
530 535 540
Asp Leu Val His Gln Ile Thr Lys Glu Lys Lys Arg Tyr Ile Leu Gly
545 550 555 560
Asn Gln
<210> 7
<211> 522
<212> PRT
<213> 宏基因组(metagenomic)
<400> 7
Met Thr His Glu Pro Thr Gln Lys Ala Leu Phe Gly Ala Phe Leu Asn
1 5 10 15
Thr Ala Gln His Asn Ala Tyr Leu Ile Ile Asn Glu Val Asn Glu Lys
20 25 30
Leu Gly Lys Ala Asp Val Glu Glu Gly Lys Leu Asp Asn Asp Ala Tyr
35 40 45
Ala Leu His Ile Leu Thr Asn Lys Glu Ile Lys Thr Leu Pro Leu Lys
50 55 60
Ile Leu Ser Arg Arg Leu Leu Met Glu Gly Phe Pro Phe Leu Cys Ala
65 70 75 80
Leu Gly Phe Ser Gln Asp Ala Thr Gln Thr Asp Asn Phe Glu Ala Leu
85 90 95
Lys Thr Lys Leu Gln Lys Ala Leu Lys Thr Leu Asn Glu Tyr Arg Asn
100 105 110
Phe Tyr Ser His Tyr Tyr Glu Gln Ser Ile Asp Trp Gln Lys Cys Gln
115 120 125
Phe Ala Asp Leu Asp Leu Ile Arg Glu Asp Phe Ile Lys Thr Phe Thr
130 135 140
Thr Tyr Ala Ser Glu Glu Gln Val Ser Lys Ser Tyr Glu Glu Val Lys
145 150 155 160
Lys Lys Lys Glu Glu Leu Asp Lys Cys Lys Lys Gly Leu Ser Leu Ala
165 170 175
Lys Arg Lys Lys Gln Thr Trp Glu Ile Asn Asp Leu Lys Gln Lys Glu
180 185 190
Glu Val Leu Arg Asp Glu Leu Leu Lys Ala Gln Gln Ala His Phe Gln
195 200 205
Leu Lys Glu Lys Lys Glu Arg Lys Glu Asn Leu Leu Lys Arg Tyr Pro
210 215 220
Asn Ile Ser Gly Ala Glu Leu Asn Lys Leu Ile Gln Asp Lys Glu Ser
225 230 235 240
Pro Tyr His Ser Phe Trp Lys Lys Asn Asn Pro His Arg Phe Ser Lys
245 250 255
Lys Gly Leu Val Phe Phe Ile Cys Leu Phe Leu Ser Lys Glu Gln Ala
260 265 270
Asn Leu Phe Leu Ser Ser Ile Ser Gly Phe Lys Arg Thr Asp Ala Ser
275 280 285
Tyr Phe Trp Ala Val Arg Ala Met Tyr Met His His Cys Cys His Leu
290 295 300
Pro Gln Pro Arg Leu Glu Ser Ser Asp Met Leu Leu Asp Ile Leu Asn
305 310 315 320
Glu Leu Asn Arg Cys Pro Lys Val Leu Tyr Asn Leu Leu Ser Glu Thr
325 330 335
Asn Arg Ala Tyr Phe Glu Lys Asp Ile Asn Tyr Lys Glu Gly Ser Val
340 345 350
Leu Gln Thr Asp Glu Glu Gly Asn Leu Val Thr Ile Gln Lys Met Leu
355 360 365
Arg His Gln Asp Arg Leu Pro Tyr Phe Ile Leu Lys Tyr Leu Asp Glu
370 375 380
Thr Asn Ala Phe Pro Asp Leu Arg Phe Gln Ile Tyr Leu Gly Lys Leu
385 390 395 400
Val Thr Asp Val Tyr Lys Lys Pro Lys Met Leu Glu Lys Asn Glu Leu
405 410 415
Gly Glu Leu Val Glu Gln Asp Gly Gln Arg Leu Ile Leu Lys Glu Val
420 425 430
His Ala Phe Gly Lys Pro Ser Asp Phe Ala Asp Lys Ile Asn Leu Pro
435 440 445
Lys Glu Leu Glu Val Asn Glu Ile Gln Asp Lys Tyr Gly Glu Met Lys
450 455 460
Gln Glu Glu Leu Lys Val Gly Thr Ile Val Gln Phe Ser Pro Gln Tyr
465 470 475 480
His Ile Ser Ser Asn Arg Ile Ala Leu Lys Leu Phe Lys Thr Lys Asp
485 490 495
Gly Lys Phe Tyr Ser Glu Lys Pro Asp Asn Asp Lys Gln Ala Arg Leu
500 505 510
Ile Ile Leu Asn Lys Asn Trp Phe Trp Phe
515 520
<210> 8
<211> 873
<212> PRT
<213> 宏基因组(metagenomic)
<400> 8
Met Gly Ile Asp Tyr Ser Leu Thr Ser Asp Cys Tyr Arg Gly Ile Asn
1 5 10 15
Lys Ser Cys Phe Ala Val Ala Leu Asn Ile Ala Tyr Asp Asn Cys Asp
20 25 30
His Lys Gly Cys Arg Thr Leu Leu Ser Glu Val Leu Arg Ser Lys Gly
35 40 45
Gly Ile Ser Asp Glu Gln Ile Lys Ser Gln Val Val Asp Gly Ile Gln
50 55 60
Lys Arg Leu Lys Asp Ile Arg Asn Tyr Phe Ser His Tyr Tyr His Ala
65 70 75 80
Glu Asp Cys Leu Arg Phe Gly Asp Gln Asp Ala Val Lys Val Phe Leu
85 90 95
Glu Glu Ile Tyr Lys Asn Ala Glu Ser Lys Thr Val Gly Ala Thr Lys
100 105 110
Glu Ser Asp Tyr Lys Gly Val Val Pro Pro Leu Phe Glu Leu His Asn
115 120 125
Gly Thr Tyr Met Ile Thr Ala Ala Gly Val Ile Phe Leu Ala Ser Phe
130 135 140
Phe Cys His Arg Ser Asn Val Tyr Arg Met Leu Gly Ala Val Lys Gly
145 150 155 160
Phe Lys His Thr Gly Lys Glu Gln Leu Ser Asp Gly Gln Lys Arg Asp
165 170 175
Tyr Gly Phe Thr Arg Arg Leu Leu Ala Tyr Tyr Ala Leu Arg Asp Ser
180 185 190
Tyr Ser Val Gly Ala Glu Asp Lys Thr Arg Cys Phe Arg Glu Ile Leu
195 200 205
Ser Tyr Leu Ser Arg Val Pro Gln Leu Ala Val Asp Trp Leu Asn Glu
210 215 220
Gln Gln Leu Leu Thr Pro Glu Glu Lys Glu Ala Phe Leu Asn Gln Pro
225 230 235 240
Ala Glu Asp Glu Gly Gly Asp Ile Ser Asp Ser Ser Ser Ser Asp Lys
245 250 255
Asn Lys Lys Ser Lys Glu Lys Arg Arg Ser Leu Arg Arg Asp Glu Lys
260 265 270
Phe Ile Leu Phe Ala Ile Gln Phe Ile Glu Gly Trp Ala Ala Glu Gln
275 280 285
Gly Leu Asp Val Thr Phe Ala Arg Tyr Gln Lys Thr Val Glu Lys Ala
290 295 300
Glu Asn Lys Asn Gln Asp Gly Lys Gln Ala Arg Ala Val Gln Leu Lys
305 310 315 320
Tyr Arg Asn Gln Gly Leu Asn Pro Asp Phe Asn Asn Glu Trp Met Tyr
325 330 335
Tyr Ile Gln Asn Glu His Ala Ile Ile Gln Ile Lys Leu Asn Asn Lys
340 345 350
Lys Ala Val Ala Ala Arg Ile Ser Glu Asn Glu Leu Lys Tyr Leu Val
355 360 365
Leu Leu Ile Phe Glu Glu Lys Gly Asn Asp Ala Val Gln Lys Leu Asn
370 375 380
Cys Tyr Ile Tyr Ser Met Ser Gln Lys Ile Glu Gly Glu Trp Lys His
385 390 395 400
Arg Pro Glu Asp Glu Arg Trp Met Pro Ser Phe Thr Lys Arg Ala Asp
405 410 415
Arg Thr Val Thr Pro Glu Ala Val Gln Ser Arg Leu Ser Tyr Ile Arg
420 425 430
Lys Gln Leu Gln Glu Thr Ile Glu Lys Ile Gly Gln Glu Glu Pro Arg
435 440 445
Asn Asn Lys Trp Leu Ile Tyr Lys Gly Lys Lys Ile Ser Met Ile Leu
450 455 460
Lys Phe Ile Ser Asp Ser Ile Arg Asp Ile Gln Arg Arg Pro Asn Val
465 470 475 480
Lys Gln Tyr His Ile Leu Arg Asp Ala Leu Gln Arg Leu Asp Phe Asp
485 490 495
Gly Phe Tyr Lys Glu Leu Gln Asn Tyr Val Asn Asp Gly Arg Ile Ala
500 505 510
Val Ser Leu Tyr Asp Gln Ile Lys Gly Val Asn Asp Ile Ser Gly Leu
515 520 525
Cys Lys Lys Val Cys Glu Leu Thr Leu Glu Arg Leu Ala Gly Leu Glu
530 535 540
Ala Lys Asn Gly Ser Glu Leu Arg Arg Tyr Ile Gly Leu Glu Ala Gln
545 550 555 560
Glu Lys His Pro Lys Tyr Gly Glu Trp Asn Thr Leu Gln Glu Lys Ala
565 570 575
Lys Arg Phe Leu Glu Ser Gln Phe Ser Ile Gly Lys Asn Phe Leu Arg
580 585 590
Lys Met Phe Tyr Gly Asp Cys Cys Gln Lys Arg Cys Phe Asp Glu Glu
595 600 605
Lys Gly Tyr Asn Thr Gln Ala Lys Glu Arg Lys Ser Leu Tyr Ser Ile
610 615 620
Val Lys Glu Lys Leu Lys Asp Ile Lys Pro Ile His Asp Asp Arg Trp
625 630 635 640
Tyr Leu Ile Asp Arg Asn Pro Lys Asn Tyr Asp Asn Lys His Ser Arg
645 650 655
Ile Ile Arg Gln Met Cys Asn Thr Tyr Ile Gln Asp Val Leu Cys Met
660 665 670
Lys Met Ala Met Trp His Tyr Glu Lys Leu Ile Ser Ala Thr Glu Phe
675 680 685
Arg Asn Lys Leu Glu Trp Asn Cys Ile Gly Gln Gly Asn Met Gly Tyr
690 695 700
Glu Arg Tyr Ser Leu Trp Tyr Lys Thr Gly Cys Gly Val Val Ile Gln
705 710 715 720
Phe Thr Pro Ala Asp Phe Leu Arg Leu Asp Ile Ile Glu Lys Pro Ala
725 730 735
Met Ile Glu Asn Ile Cys Gln Cys Phe Val Leu Gly Asn Lys Lys Leu
740 745 750
Asn Ser Gly Ala Glu Lys Lys Ile Thr Trp Asp Lys Phe Asn Lys Asp
755 760 765
Gly Ile Ala Lys Tyr Arg Lys Arg Gln Ala Glu Ala Val Arg Ala Ile
770 775 780
Phe Ala Phe Glu Glu Gly Leu Lys Ile Gln Glu Asp Lys Trp Ser His
785 790 795 800
Glu Arg Tyr Phe Pro Phe Cys Asn Ile Leu Asp Glu Ala Val Lys Gln
805 810 815
Gly Lys Ile Lys Asp Thr Gly Lys Asp Lys Glu Ala Leu Asn Arg Gly
820 825 830
Arg Asn Asp Phe Phe His Glu Glu Phe Lys Ser Thr Glu Asp Gln Gln
835 840 845
Ala Ile Phe Gln Lys Tyr Phe Pro Ile Val Glu Arg Lys Asp Asp Thr
850 855 860
Lys Lys Arg Arg Asp Lys Lys Gln Lys
865 870
<210> 9
<211> 533
<212> PRT
<213> 宏基因组(metagenomic)
<400> 9
Met Ser Gly Leu Pro Leu Pro Lys Pro Leu Arg Arg Trp Leu Leu Gly
1 5 10 15
Thr Leu Ser Asn Gln Glu Arg Lys Ala Gln Asp Trp Leu Gln Ser Lys
20 25 30
Leu Glu Glu Asn Leu Arg Leu Leu Leu Glu Ala Lys Arg Gln Leu Arg
35 40 45
Lys Leu Ala Glu Pro Pro Asn Arg Pro Phe Leu Arg Ala Gly Asp Leu
50 55 60
Ala Thr Trp Ile Ala Arg Asp Ile Ala Tyr Trp Gln His Pro Asp Gly
65 70 75 80
Arg Gln Leu Asn Ser Glu Glu Tyr Gly Glu Leu Gln Ala Ala Leu Ala
85 90 95
Phe Phe Gly Arg Glu Gly Ala Glu Gln Val Tyr Glu Leu Phe Glu Lys
100 105 110
Leu Glu Leu Val Gly Gln Pro Asn Arg Asp His Pro Phe Leu Phe Arg
115 120 125
Leu Lys Asn Arg Met Cys Pro Ala Arg Ser Arg Lys Arg Gln Pro Lys
130 135 140
Pro Ile Arg Gly Ile Leu Glu Phe Tyr Ile Ala Tyr Leu Arg Ala Arg
145 150 155 160
Glu Lys Phe Leu Thr Arg Leu Gln Gly Arg Leu Lys Ser Gly Ser Asn
165 170 175
Pro Thr Pro Glu Met Glu His Trp Ile Pro Pro Leu Arg Ser Arg Ser
180 185 190
Leu Pro Pro Arg Met Asn Glu Arg Ala Val Val Leu Pro Arg Gly Leu
195 200 205
Phe Arg Arg Arg Phe Val Glu Leu Leu Pro Pro Pro Asp Gly Ser Pro
210 215 220
Ala Asp Thr Pro Leu Glu Gln Leu Thr Leu Met Lys Leu Leu Glu Pro
225 230 235 240
Tyr Leu Asp Thr Pro Gln Ala Phe Tyr Arg Tyr Glu Arg Arg Tyr Ala
245 250 255
Arg Asn Glu His Gln Tyr Thr Thr Asp Gly Cys Asn Leu Ser Glu Leu
260 265 270
Ile Pro Gln Ile Glu Asn Glu Ala Thr Gln Gly Ser Glu Glu Glu Asp
275 280 285
Pro Asp Phe Arg Arg Lys Asp Leu Leu Cys Leu Ala Arg Thr Ile Asn
290 295 300
Asp Thr Glu Arg Arg Leu Arg Arg Tyr Gln Leu Gln Asp Arg Leu Leu
305 310 315 320
Trp Met Ile Val Arg Gln Glu Leu Glu Arg Glu Lys Asn Met Asn Leu
325 330 335
Gln Gly Trp Thr Leu Glu Asn Val Gly Phe Asp Gln Pro Asp Ser Pro
340 345 350
Leu Lys Gln Glu Ile Thr Phe Gln Leu Glu Tyr Pro Leu Glu Gly Arg
355 360 365
Asn Glu Pro Ile Arg Ile Glu Ala Lys Leu Arg Pro Arg His Tyr Gly
370 375 380
Lys Met Arg Arg Thr Leu His Asp Arg Arg Leu Pro Gly Leu Leu Gln
385 390 395 400
Trp Leu Asn Thr Glu Ser Val Asp Trp Glu Arg Leu Gln Gln Glu Leu
405 410 415
Thr Lys Thr Ile Thr Arg Gln Asp Glu Val Leu Gln Leu Ile Phe Glu
420 425 430
Leu Glu Lys Ala Val His Ser Arg Phe Glu Lys Glu Cys Thr Ala Lys
435 440 445
Lys Asn Arg Asp Tyr Leu Lys His Arg Asn Tyr Leu Glu Val Leu Arg
450 455 460
Glu His Gln Ile Leu Arg Ser Asp Glu Glu Phe Glu His Leu Leu Asn
465 470 475 480
Leu Arg Asn Arg Phe Ala His Asn Glu Val Pro Ser Pro Ala Glu Leu
485 490 495
Pro Leu Trp Lys Asp Glu Arg Pro Gly Asn Gly Asn Leu Met Glu Trp
500 505 510
Leu Ile Glu Lys Ser Leu Gln Leu Tyr Arg Asp Leu Leu Glu Arg Val
515 520 525
Gln Gly Lys Gly Asp
530
<210> 10
<211> 841
<212> PRT
<213> 宏基因组(metagenomic)
<400> 10
Met Pro Val Asn Tyr Ser Leu Asp Gln Asp Tyr Tyr Lys Gly Thr His
1 5 10 15
Lys Ser Cys Phe Thr Val Pro Leu Asn Ile Ala Trp Asp Asn Gly Ser
20 25 30
Lys Lys Gly Cys Glu Asn Leu Leu Lys Glu Ala Met Arg Thr Arg Gly
35 40 45
Gly Phe Thr Gln Glu Asp Ile Glu Lys Val His Arg Ser Leu Ala Glu
50 55 60
Lys Leu Asn Gly Ile Arg Asp Tyr Phe Ser His Tyr Tyr His Glu Asp
65 70 75 80
Lys Pro Leu Glu Phe Lys Lys Gly Asp Asp Asp Ala Val Lys Asp Phe
85 90 95
Leu Glu Lys Thr Phe Ser Tyr Ala Ala Gly Glu Thr Gln Lys Arg Val
100 105 110
Lys Glu Ser Gly Tyr Gln Gly Ile Ile Pro Pro Ile Phe Glu Leu Cys
115 120 125
Gly Asp Gln Val Arg Ile Thr Ala Ala Gly Val Ile Phe Leu Ala Ser
130 135 140
Phe Phe Val Pro Arg Ser Thr Leu Glu Arg Met Phe Gly Ala Val Gln
145 150 155 160
Gly Phe Lys Arg Ser Asp Arg Gly Asp Leu Asp Thr Gly Gln Lys Arg
165 170 175
Asp Tyr Tyr Phe Thr Arg Ser Leu Leu Ser Phe Tyr Thr Leu Arg Asp
180 185 190
Ser Tyr Tyr Leu Gln Ala Asp Glu Thr Arg Pro Phe Arg Glu Ile Leu
195 200 205
Ser Tyr Leu Ser Cys Val Pro Phe Asp Ser Val Gln Trp Leu Gln Ala
210 215 220
His Gly Lys Leu Ser Lys Ser Glu Glu Lys Glu Phe Phe Gly Arg Pro
225 230 235 240
Val Glu Glu Gln Asp Glu Glu Asn Pro Ala Gln Thr Glu Lys Gln Thr
245 250 255
Ala Pro Ala Gly Arg Arg Met Arg Lys Lys Asn Lys Phe Ile Leu Phe
260 265 270
Ala Val Arg Phe Ile Glu Ala Trp Ala Arg Asn Glu Lys Leu Ser Val
275 280 285
Glu Phe Gly Arg Tyr Arg Asn Ile Gln Asn Glu Glu Asp Arg Arg Lys
290 295 300
Gln Ser Gly Lys Lys Val Arg Glu Val Phe Phe Pro Ser Ala Leu Asn
305 310 315 320
Asn Leu Ser Ala Glu Glu Gln Asp Leu Glu Gly Leu Leu Tyr Ile Arg
325 330 335
Asn Asn His Ala Leu Ile Arg Ile His Leu Lys Ala Lys Thr Pro Val
340 345 350
Thr Val Arg Ile Ser Glu His Glu Leu Met Tyr Leu Val Leu Ala Ile
355 360 365
Leu Ser Gly Lys Gly Gly Asn Ala Val Gln Lys Leu Ser Lys Tyr Val
370 375 380
Trp Asp Val Arg Met Arg Ser Arg Gly Pro Leu Thr Asn Met Pro Arg
385 390 395 400
Asn Phe Pro Ala Phe Leu Arg Ser Pro Ala Ser Glu Val Ser Glu Gln
405 410 415
Ala Val Gln Asn Arg Leu Asn Tyr Ile Arg Lys Thr Leu Lys Glu Ile
420 425 430
Gln Ala Asn Leu Gln Lys Glu Ala Gln Thr Gly Gln Trp Ile Leu Asp
435 440 445
Lys Gly Gln Lys Ile Arg His Ile Leu Arg Phe Ile Ser Asp Ser Met
450 455 460
Pro Asp Phe Arg Arg Arg Pro Ser Val Lys Glu Tyr Asn Glu Leu Arg
465 470 475 480
Glu Leu Leu Gln Thr Leu Ala Phe Asp Asp Phe Tyr Arg Lys Leu Ala
485 490 495
Ser Phe Gln Thr Glu Arg Lys Leu Asp Ala Ala Val Trp Asn Asn Leu
500 505 510
Ala Gln Cys Lys Ser Ile Asn Glu Leu Cys Glu Arg Cys Cys Gln Leu
515 520 525
Gln Gln Gln Arg Leu Asp Glu Leu Glu Lys Gln Gly Gly Asp Glu Leu
530 535 540
Lys Arg Tyr Ile Gly Leu Leu Pro Lys Glu Lys Gly Lys His Tyr Glu
545 550 555 560
Glu Gln Asn Thr Pro Ala Arg Lys Phe Glu Arg Phe Ile Glu Asn Gln
565 570 575
Leu Ser Val Pro Lys Tyr Phe Leu Arg Cys Lys Leu Phe Val Thr Gly
580 585 590
Gly Ser Arg Arg Thr Asn Leu Leu Lys Leu Val Gln Glu His Leu Lys
595 600 605
Pro Lys Thr Ser Val Phe His Glu Glu Arg Leu Tyr Leu Arg Glu Glu
610 615 620
Gln Pro Gly Asp Tyr Pro Trp Ser Asp Arg Lys Ile Ile Gln Lys Met
625 630 635 640
Tyr Tyr Leu Tyr Val Gln Asp Leu Leu Cys Met Gln Met Ala Gln Trp
645 650 655
His Tyr Glu His Leu Thr Pro Gln Val Lys Gly Lys Ile Asp Trp Glu
660 665 670
Ile Asn Ser Glu Ser Lys Glu Ser Asp Gly Tyr Asn Arg Phe Lys Val
675 680 685
Glu Tyr Lys Gly Pro Gln Gly Cys Arg Ile Ile Phe Arg Val Gln Asp
690 695 700
Phe Gly Arg Leu Asp Phe Leu Asn Lys Ala Pro Met Leu Asp Asn Ile
705 710 715 720
Cys Gln Trp Phe Leu Ser Gly Arg Lys Glu Ile Thr Trp Pro Glu Phe
725 730 735
Leu Arg Asp Gly Leu Gln Arg Tyr Arg Gln Arg Gln Ile Leu Val Val
740 745 750
Arg Ala Leu Phe Arg Phe Glu Glu Asn Leu Lys Ile Pro Glu Glu Glu
755 760 765
Trp Lys Gly Lys Ser His Leu Ser Phe Asp Glu Val Leu Glu Arg Phe
770 775 780
Ser Gly Lys Asn Arg Leu Ser Glu Glu Glu Lys Glu Ser Ile Arg Arg
785 790 795 800
Val Arg Asn Asp Phe Phe His Glu Glu Phe Glu Ala Thr Pro Ser Gln
805 810 815
Trp Arg Asp Phe Glu Arg Arg Met Ser Glu Tyr Leu Asn Lys Glu Lys
820 825 830
Arg Glu Lys Pro Lys Lys Lys Lys Arg
835 840
<210> 11
<211> 840
<212> PRT
<213> 宏基因组(metagenomic)
<400> 11
Met Arg Glu Asn Thr Tyr Ser Lys Gln Gln Lys Ser Lys Pro Lys Thr
1 5 10 15
Thr Thr Asp Tyr Gln Gly Ala Asp Lys Trp Cys Phe Ala Ile Val Leu
20 25 30
Asn Arg Ala Cys Asp Asn Tyr Lys Asp Asn His His Leu Phe Pro Glu
35 40 45
Ser Leu Leu Arg Tyr Glu Ser Val Asn Arg Arg Asn Trp Phe Glu Glu
50 55 60
Glu Ile Arg Thr Leu Ile Val Lys Met Asp Thr Glu Asp Pro Ser Leu
65 70 75 80
Arg Pro Arg Asp Pro Lys Lys Val Ile Ala Gly Ile Asn Tyr Ser Pro
85 90 95
Val Asn Arg Arg Leu Asn Asp Val Arg Asn Tyr Phe Ser His His His
100 105 110
His Glu Pro Ser Pro Leu Glu Phe Leu Lys Asp Asp Pro Ile Arg Lys
115 120 125
Ile Met Glu Lys Ala Tyr Glu Lys Ala Lys Phe Glu Glu Ile Lys Arg
130 135 140
Gln Tyr Arg Glu Pro Ser Ile Thr Phe Pro Asp Leu Phe Asp Ala Lys
145 150 155 160
Asn Gln Ile Thr Pro Ala Gly Ile Ile Phe Phe Ala Ser Phe Phe Val
165 170 175
Glu Arg Arg Ile Leu Ala Arg Leu Met Gly Ser Ile Gly Gly Phe Thr
180 185 190
Lys Thr Glu Gly Glu Tyr Asn Ile Thr Arg Asp Ile Phe Thr Thr Tyr
195 200 205
Cys Leu Lys Asp Ser Tyr Ser Ile His Thr Gln Asp Ala Lys Asp Val
210 215 220
Leu Phe Arg Asp Ile Ile Gly Tyr Leu Ser Arg Val Pro Ser Glu Tyr
225 230 235 240
Tyr Lys Tyr Asn Lys Asp His Cys Asp Lys Glu Gly Val Pro Glu Arg
245 250 255
Lys Thr Asp Lys Phe Ile Thr Phe Ala Leu Gln Tyr Leu Glu Ala Phe
260 265 270
Ala Ala Lys Glu Ala Lys Glu Tyr Thr Ile Ser Val Gly Arg Met Glu
275 280 285
Ile Val Arg Glu Glu Arg Lys Glu Ile Val Lys Gly Asp Lys Thr Phe
290 295 300
Lys Pro Cys Pro Asn Lys Gly Lys Ile Lys Val Leu Phe Asp Thr Gly
305 310 315 320
Ala Asn Asn Leu Pro Tyr Tyr Ile Asn His Asn Thr Ala Ile Leu Gln
325 330 335
Phe Gln Lys Asn Gly Gly Ser Arg Tyr Ser Cys Lys Ile Gly Ile Asn
340 345 350
Glu Leu Lys Tyr Leu Val Leu Leu Cys Ile Gln Gly Lys Thr Lys Glu
355 360 365
Ala Ala Glu Ala Leu Tyr His Tyr Leu Asp Glu Ile Gln Glu Arg Phe
370 375 380
Lys Asp Pro Ala Thr Lys Val Gly Ala Asp Asp Ser Glu Arg Phe Lys
385 390 395 400
Gln Arg Leu Pro Gly Phe Ile Lys Leu His Ser Asn Leu Glu Val Ala
405 410 415
Asp Arg Asp Ala Ala Ile Lys Ala Arg Tyr Asp Tyr Ile Ile Asn Lys
420 425 430
Trp Asn Lys Lys Arg Gly Thr Gly Asn Glu Ile Met Ala Asp Ala Lys
435 440 445
Leu His Gln Lys Ala Arg Asp Ile Leu Arg Tyr Ile Lys Trp His Ile
450 455 460
Ser Arg Pro Leu Ser Thr Gly Glu Tyr Asn Phe Leu Leu Ala Leu Leu
465 470 475 480
Ile Glu Lys Asn Phe Asp Lys Phe Asp Glu Arg Ile Thr Glu Leu Trp
485 490 495
Arg Thr Glu Arg Ile Thr Gln Glu Ile Leu Gly Gln Leu Arg Gly Asn
500 505 510
Lys Ser Leu Asp Met Leu Tyr Glu Lys Val Trp Ser Tyr Val Arg Asn
515 520 525
Glu Leu Gln Thr Leu Lys Glu Asn Asp Ile Ser Lys Leu Ala Glu Tyr
530 535 540
Ile Gly Val Val Arg Thr Pro Ala Glu Asn Ala Pro Thr Tyr Glu Thr
545 550 555 560
Lys Val Lys Thr Phe Ala Ala Gln Pro Met Ile Cys Lys Gly Tyr Leu
565 570 575
Arg Glu Gln Phe Phe Gly Thr Glu Lys Thr Phe Ala Lys Leu Val Glu
580 585 590
Asn Gln Leu Gly Thr Asn Pro Asp Val Pro Leu Gly Lys Gln Phe Tyr
595 600 605
His Val Ser Ser Leu Asp Gln Phe Ser Lys Asp Asn Ala Val Leu Tyr
610 615 620
Glu Thr Leu Ala Leu Asp Arg Leu Cys Val Met Met Ala Arg Lys Cys
625 630 635 640
Phe Ala Asp Ile Asn Ala Asn Leu Arg Thr Lys Gly Gln Glu Leu Arg
645 650 655
Trp Lys Asp Asn Asp Gly Lys Glu Leu Ile Glu Ile Asp Ile Pro Arg
660 665 670
Glu Asn Asn Ile Ser Thr Ala Arg His Val Ser Pro Arg Gly Phe Gly
675 680 685
Glu Thr Leu Lys Ile Pro Gln Thr Ser Ala Thr Arg Asp Val Phe Thr
690 695 700
Ile Arg Phe Asp Arg Lys His Tyr Thr Lys Leu Tyr Val Lys Asp Asp
705 710 715 720
Ala Glu Phe Leu Ala Gly Leu Met Lys Tyr Phe Phe Pro Gly Glu Thr
725 730 735
Val Lys Asp Tyr His Leu Leu Tyr Ser Asp Gly Ile Asn His Tyr Thr
740 745 750
Glu Leu Gln Arg Gln Ala Ile Thr Ser Leu Phe Glu Leu Glu Gly Glu
755 760 765
Ile Ile Glu Arg Ala Lys Met Asn Lys Ser Lys His Tyr Ile Arg Phe
770 775 780
Gly Asp Ile Met Lys Lys Ser Glu Phe Lys Pro Asn Glu Gln Asp Ala
785 790 795 800
Val Thr Asn Val Arg Asn Ser Leu Leu His Tyr Glu Leu Lys Phe Thr
805 810 815
Val Lys Asp Phe Arg Ile Phe Val Gln Val Met Asn Arg Glu Lys Met
820 825 830
Asn Lys Asp Trp Val Leu Lys Val
835 840
<210> 12
<211> 840
<212> PRT
<213> 宏基因组(metagenomic)
<400> 12
Met Ala Val Asp Tyr Ser Leu Lys Gln Pro Phe Tyr Gln Gly Val His
1 5 10 15
Lys Ser Cys Phe Thr Val Pro Leu Asn Ile Ala Ala Asp Asn Cys Lys
20 25 30
Gln Lys Gly Tyr Arg Asn Leu Leu Lys Glu Ala Gln Arg Ser Lys Gly
35 40 45
Gly Leu Ser Asp Gln Ser Ile Gln Glu Ala Ala Asp Leu Ile Glu Lys
50 55 60
Arg Leu Ser Ala Ile Arg Asn Tyr Phe Ser His Thr Tyr His Thr Asp
65 70 75 80
Ser Val Leu Thr Phe Gln Lys Glu Asp Pro Val Lys Lys Phe Leu Glu
85 90 95
Thr Ala Trp Ser Tyr Ala Val Ser Glu Thr Gln Lys Asp Ile Ala Glu
100 105 110
Ser Asp Tyr Thr Gly Ile Val Pro Pro Leu Phe Glu Asp Lys Glu Gly
115 120 125
Gln Phe Gln Ile Thr Ala Ala Gly Val Ile Phe Leu Met Ser Phe Phe
130 135 140
Cys His Arg Ser Val Leu Asn Arg Met Phe Gly Ser Val Lys Gly Leu
145 150 155 160
Lys Arg Ser Asp Arg Glu Gln Met Gly Thr Gly Glu Lys Arg Asp Tyr
165 170 175
Gln Phe Thr Arg Lys Leu Leu Ser Phe Tyr Ser Leu Arg Asp Ser Tyr
180 185 190
Ala Val Lys Ala Glu Ala Thr Arg Pro Phe Arg Glu Ile Leu Ser Tyr
195 200 205
Leu Ser Cys Val Pro His Glu Ser Leu Val Trp Leu Ser Ala Arg Gly
210 215 220
Lys Leu Thr Glu Lys Glu Lys Lys Ala Phe Arg His Phe Leu Asp Pro
225 230 235 240
Thr Val Pro Lys Glu Ala Leu Ser Glu Glu Ser Ala Gly Asp Gly Ser
245 250 255
Asp Ser Glu Arg Pro Gly Val Arg Lys Asn Asn Lys Phe Leu Leu Phe
260 265 270
Ala Val Gln Phe Ile Glu Ala Trp Ser Arg Lys Glu Lys Lys Gly Leu
275 280 285
Glu Phe Ala Arg Tyr Arg Lys Ser Arg Val Glu Ala Pro Gly Glu Asn
290 295 300
Gln Asp Gly Ser Glu Lys Arg Ile Val Arg Phe Arg Ser Glu Ile Arg
305 310 315 320
Asp Thr Gln Glu Asp Trp Pro Tyr Tyr Leu Arg Asn Asn His Ala Leu
325 330 335
Leu Arg Leu His Pro Gly Glu Asn Lys Glu Pro Val Asp Ala Arg Ile
340 345 350
Gly Glu Tyr Glu Leu Leu Tyr Leu Val Leu Ala Ile Phe Asp Gly Lys
355 360 365
Gly Ala Lys Ala Ile Gln Lys Leu Ala Asn Tyr Ile Phe Glu Ala Lys
370 375 380
Lys Gln Ile Gln Asn Ala Arg Val Tyr Asp Arg Tyr Gln Asp Leu Leu
385 390 395 400
Pro Ser Phe Leu Thr Ala Gly Asn Lys Pro Val Ser Ala Glu Thr Ile
405 410 415
Arg Asn Arg Leu Ala Tyr Ile Arg Gly Glu Leu Glu Lys Met Leu Glu
420 425 430
Ala Val Gln Lys Glu Lys Lys Ser Gly Arg Trp Glu Met His Lys Gly
435 440 445
Lys Lys Ile Gly His Ile Leu Arg Phe Leu Ser Asn Ser Ile Asp Asp
450 455 460
Ile Arg Arg Arg Pro Asn Val Lys Glu Tyr Asn Arg Leu Arg Asp Leu
465 470 475 480
Leu Gln Gln Leu Gln Trp Asp Glu Phe Asp Lys Ala Leu Gln Ser Tyr
485 490 495
Val Asn Glu Lys Leu Leu Asp Glu Thr Val Tyr Arg Gln Leu Arg Gly
500 505 510
Phe His Ser Leu Asp Glu Leu Phe Glu Arg Cys Cys Arg Leu Glu Leu
515 520 525
Lys Arg Leu Glu Asp Met Glu Lys Ala Gly Gly Asp Arg Leu Asn Arg
530 535 540
Tyr Ile Gly Leu Glu Pro Lys Gly Lys Pro Lys Asn Tyr Ala Asp Leu
545 550 555 560
Asn Thr Leu Gln Lys Lys Gly Glu Arg Phe Leu Lys Gly His Gln Leu
565 570 575
Ser Ile Pro Arg Tyr Phe Leu Arg Asn Ala Leu Tyr Lys Glu Tyr Gln
580 585 590
Ala Thr Glu Glu Arg Lys Pro Thr Ser Leu Tyr Gln Ile Val Arg Glu
595 600 605
Arg Leu Pro Arg Thr Asn Pro Ile Leu Pro Asp Arg Tyr Tyr Leu Leu
610 615 620
Glu Glu Asp Pro Lys Thr Tyr Ser Gly Ser Asp Ser Lys Ile Ile Arg
625 630 635 640
Glu Met Cys Phe Thr Tyr Ile Glu Asp Leu Leu Cys Met Arg Met Ala
645 650 655
Arg Trp His Tyr Glu Gln Leu Ser Glu Lys Leu Arg Lys Lys Leu Gln
660 665 670
Trp Lys Glu Val Gln Thr Gly Pro Ala Gly Tyr Glu Arg Phe Arg Leu
675 680 685
Ile Tyr Lys Ile Ser Asp Glu Leu Ser Ile Glu Phe His Pro Ser Asp
690 695 700
Leu Thr Arg Leu Asp Val Ile Glu Lys Asp Asp Met Leu Thr Asn Ile
705 710 715 720
Ser Gln His Phe Leu Thr Lys Lys Gly Thr Val Arg Trp Thr Glu Phe
725 730 735
Val Ser Gln Gly Met Lys His Tyr Arg Asp Arg Gln Lys Gln Gly Ile
740 745 750
Glu Ala Leu Phe Lys Trp Glu Glu Ser Leu Arg Ile Pro Glu Gly Leu
755 760 765
Trp Lys Glu Glu Gly Tyr Leu Gly Phe Glu Lys Val Leu Glu Glu Ala
770 775 780
Val Lys His Gly Lys Ile Gln Asp Lys Asp Lys Glu Ala Leu Lys Arg
785 790 795 800
Ile Arg Asn Asp Phe Phe His Glu His Phe Cys Gly Thr Pro Ala Asp
805 810 815
Trp Glu Val Phe Lys Arg Val Leu Lys Arg Phe Leu Asn Gln Gly Lys
820 825 830
Asn Glu Lys Lys Arg Phe Lys Lys
835 840
<210> 13
<211> 838
<212> PRT
<213> 宏基因组(metagenomic)
<400> 13
Met Gln Phe Glu Asn Ile Lys Asp Thr Gly Gln Lys Pro Ile Tyr Ser
1 5 10 15
Ile Asp Gln Tyr Glu Gly Ala Lys Lys Trp Cys Phe Ala Ile Val Leu
20 25 30
Asn Arg Ala Cys Asp Asn Tyr Glu Asp Asn Pro Gln Leu Phe Ser Glu
35 40 45
Ser Leu Leu Arg Phe Glu Glu Val Asn Arg Arg Asp Trp Phe Asp Lys
50 55 60
Asp Ile Arg Asp Leu Ile Lys Lys Ala Asp Thr Glu Asp Gln Ile Glu
65 70 75 80
Pro Lys Arg Lys Pro Asn Thr Pro Val Asn Arg Arg Leu His Asp Ile
85 90 95
Arg Asn Tyr Phe Ser His Ser Arg His Gln Asp Asp Cys Leu Tyr Phe
100 105 110
Lys Asn Asp Asp Pro Met Arg Cys Ile Met Glu Ala Ala Tyr Glu Lys
115 120 125
Ala Lys Ile His Ile Lys Gly Arg Gln Thr Glu Gln Ser Asp Ile Pro
130 135 140
Leu Pro Glu Leu Phe Asp Ala Asn Asn Lys Ile Thr Ser Ala Gly Val
145 150 155 160
Leu Phe Leu Ala Ser Phe Phe Val Glu Arg Gly Ile Leu His Arg Leu
165 170 175
Met Gly Asn Ile Gly Gly Phe Lys Asp Asn Arg Gly Lys Tyr Gly Leu
180 185 190
Thr His Asp Ile Phe Thr Thr Tyr Cys Leu Lys Asp Ser Tyr Ser Ile
195 200 205
His Ala Ser Asp Pro Lys Val Val Leu Phe Arg Asp Ile Ala Gly Tyr
210 215 220
Leu Ser Leu Val Ala Cys Glu Tyr Tyr Pro Thr Tyr Leu Ser Lys Ile
225 230 235 240
Pro Lys Glu Asn Ala Gly Glu Lys Ser Ser Asp Glu Glu Lys Tyr Ala
245 250 255
Glu Arg Lys Thr Asp Lys Phe Ile Leu Phe Ala Leu Lys Tyr Leu Glu
260 265 270
Glu Phe Val Leu Pro Ser Leu Lys Asp Asp Tyr Leu Val Asp Ile Gly
275 280 285
Arg Ile Asp Ile Ile Arg Glu Glu Ser Lys Glu Thr Glu Glu Lys Asp
290 295 300
Glu Gln Tyr Lys Pro His Pro Asn Gln Gly Lys Val Lys Val Val Phe
305 310 315 320
Asp Ser Ile Asn Lys Glu Leu Pro Tyr Tyr Ile Asn His Asn Thr Val
325 330 335
Ile Leu Arg Ile Gln Lys Asn Gly Val Met Ala Tyr Ser Cys Lys Ile
340 345 350
Gly Val Asn Asp Leu Lys Tyr Leu Leu Leu Leu Cys Leu Gln Gly Lys
355 360 365
Thr Asp Lys Ala Leu Asp Ala Ile Tyr Asn Tyr Leu His Ser Met Gln
370 375 380
Asp Pro Pro Glu Val Val Lys Ile Gly Ala Thr Asp Lys Leu Phe Gln
385 390 395 400
Gly Leu Pro Glu Phe Ile Leu Lys Gln Ser Gly Ile Lys Val Gln Asp
405 410 415
Lys Asn Lys Glu Lys Ala Ala Arg Ile Lys Tyr Ile Arg Asp Lys Trp
420 425 430
Glu Lys Lys Lys Ser Glu Ser Ala Asp Met Glu Leu His Arg Lys Gly
435 440 445
Arg Asp Ile Leu Arg Tyr Val Asn Trp His Cys Glu Thr Pro Leu Gly
450 455 460
Thr Glu Lys Tyr Asp Gln Leu Leu Val Leu Leu Val Asn Lys Asn Phe
465 470 475 480
Val Val Phe Gly Asp Glu Leu Asn Gln Leu Lys Arg Thr Glu Ile Ile
485 490 495
Ser Lys Asp Ile Leu Glu Lys Leu Ser Gly Phe Gln Thr Ile Asn Thr
500 505 510
Leu His Gln Lys Val Cys Asn Leu Val Leu Glu Glu Leu Ser Ser Leu
515 520 525
Glu Lys Asn Asp Pro Gly Lys Leu Ala Glu His Ile Gly Leu Val Arg
530 535 540
Lys Pro Ala Pro Glu Asn Asn Pro Pro Pro Glu Tyr Lys Glu Lys Val
545 550 555 560
Arg Arg Phe Val Glu Gln Pro Met Ile Tyr Lys Gly Phe Leu Arg Asp
565 570 575
Gln Phe Phe Val Asn Lys Asp Gln Asp Gly Lys Lys Leu Lys Glu Gln
580 585 590
Lys Thr Phe Ala Lys Leu Val Glu Glu Thr Leu Gly Gln Asn Ala Asp
595 600 605
Val Pro Leu Gly Lys Asp Phe Tyr Tyr Val Pro Asn Ile Glu Lys Asp
610 615 620
Glu Lys Lys Asn Arg Phe His Lys Asp Asn Ala Val Leu Tyr Glu Thr
625 630 635 640
Leu Ala Leu Asp Arg Leu Cys Ala Met Met Ala Arg Lys Cys Leu Thr
645 650 655
Gln Ile Asn Lys Asn Leu Ala Glu Lys Ser Glu Glu Ile Asp Trp Arg
660 665 670
Asn Glu Asp Gly Lys Asp Phe Ile Tyr Leu Lys Leu Val Lys Ser Asp
675 680 685
Arg Pro Gln Glu Thr Phe Lys Ile Arg Phe Lys Val Asn Asp Phe Ala
690 695 700
Lys Leu Tyr Val Met Asp Asp Pro Asp Phe Leu Gly Gly Leu Met Lys
705 710 715 720
His Phe Phe Pro Gln Glu His Ser Ile Glu Tyr His Lys Leu Tyr Arg
725 730 735
Asn Gly Ile Glu Arg Tyr Thr Asp Arg Gln Lys Asp Gly Ile Glu Ala
740 745 750
Ile Leu Arg Leu Glu Asp Ser Val Ile Arg Gln Lys Gly Met Lys Pro
755 760 765
Lys Pro Ala Lys Asn Tyr Ile Ser Phe Ser Glu Ile Met Ala Gln Thr
770 775 780
Asp Tyr Pro Glu His Asp Gln Lys Val Leu Asn Lys Val Arg Arg Ala
785 790 795 800
Val Leu His Tyr His Leu Lys Phe Glu Pro Ala Asp Tyr Asn Arg Phe
805 810 815
Val Asp Ile Met Lys Lys Asn Lys Phe Trp Asp Gly Glu Arg Lys Asn
820 825 830
Lys Glu Ser Arg Gly Arg
835
<210> 14
<211> 804
<212> PRT
<213> 宏基因组(metagenomic)
<400> 14
Met Glu Phe Glu Asn Ile Lys Lys Thr Ser Asn Lys Glu Val Tyr Ser
1 5 10 15
Ile Glu Gln Tyr Glu Gly Glu Lys Lys Trp Cys Phe Ala Ile Val Leu
20 25 30
Asn Arg Ala Gln Thr Asn Leu Glu Glu Asn Pro Lys Leu Phe Glu Gln
35 40 45
Thr Leu Thr Arg Phe Glu Lys Ile Met Lys Gln Asp Trp Phe Asn Glu
50 55 60
Glu Thr Lys Lys Leu Ile Tyr Glu Lys Glu Glu Glu Asn Lys Val Lys
65 70 75 80
Glu Glu Ile Gln Ile Ala Ala Ser Glu Arg Leu Lys Asn Leu Arg Asn
85 90 95
Tyr Phe Ser His Tyr Leu His Ala Pro Asp Cys Leu Ile Phe Asn Arg
100 105 110
Asn Asp Thr Ile Arg Ile Ile Met Glu Lys Ala Tyr Glu Lys Ser Arg
115 120 125
Phe Glu Ala Lys Lys Lys Gln Gln Glu Asp Ile Ser Ile Glu Phe Pro
130 135 140
Glu Leu Phe Glu Glu Glu Asp Lys Ile Thr Ser Ala Gly Val Val Phe
145 150 155 160
Phe Val Ser Phe Phe Ile Glu Arg Arg Phe Leu Asn Arg Leu Met Gly
165 170 175
Tyr Val Gln Gly Phe Arg Lys Thr Glu Gly Glu Tyr Asn Ile Thr Arg
180 185 190
Gln Val Phe Ser Lys Tyr Cys Leu Lys Asp Ser Tyr Ser Val Gln Ala
195 200 205
Gln Asp His Asp Ala Val Met Phe Arg Asp Ile Leu Gly Tyr Leu Ser
210 215 220
Arg Val Pro Thr Glu Ile Tyr Gln His Ile Lys Leu Thr Arg Lys Arg
225 230 235 240
Ser Gln Asp Gln Leu Ser Glu Arg Lys Thr Asp Lys Phe Ile Leu Phe
245 250 255
Ala Leu Lys Tyr Leu Glu Asp Tyr Gly Leu Lys Asp Leu Ala Asp Tyr
260 265 270
Thr Ala Cys Phe Ala Arg Ser Lys Ile Lys Arg Glu Asn Glu Asp Thr
275 280 285
Lys Glu Thr Asp Gly Asn Lys His Lys Phe His Arg Glu Lys Pro Val
290 295 300
Val Glu Ile His Phe Asp Lys Glu Lys Gln Asp Gln Phe Tyr Ile Lys
305 310 315 320
Arg Asn Asn Val Ile Leu Lys Ala Gln Lys Lys Gly Gly Gln Ser Asn
325 330 335
Val Phe Arg Met Gly Val Tyr Glu Leu Lys Tyr Leu Val Leu Leu Ser
340 345 350
Leu Leu Gly Lys Ala Glu Glu Ala Ile Gln Arg Ile Asp Arg Tyr Ile
355 360 365
Ser Ser Leu Lys Lys Gln Leu Pro Tyr Leu Asp Lys Ile Ser Asn Glu
370 375 380
Glu Ile Gln Lys Ser Ile Asn Phe Leu Pro Arg Phe Val Arg Ser Arg
385 390 395 400
Leu Gly Leu Leu Gln Val Asp Asp Glu Lys Arg Leu Lys Thr Arg Leu
405 410 415
Glu Tyr Val Lys Ala Lys Trp Thr Asp Lys Lys Glu Gly Ser Arg Lys
420 425 430
Leu Glu Leu His Arg Lys Gly Arg Asp Ile Leu Arg Tyr Ile Asn Glu
435 440 445
Arg Cys Asp Arg Pro Leu Ser Arg Lys Glu Tyr Asn Asn Ile Leu Lys
450 455 460
Phe Ile Val Asn Lys Asp Phe Ala Gly Phe Tyr Asn Glu Leu Glu Glu
465 470 475 480
Leu Lys Arg Thr Arg Arg Leu Asp Lys Asn Ile Ile Gln Lys Leu Ser
485 490 495
Gly His Thr Thr Leu Asn Ala Leu His Glu Arg Val Cys Asp Leu Val
500 505 510
Leu Gln Glu Leu Gly Ser Leu Gln Ser Glu Asn Leu Lys Glu Tyr Ile
515 520 525
Gly Leu Ile Pro Lys Glu Glu Lys Glu Val Thr Phe Arg Glu Lys Val
530 535 540
Asp Arg Ile Leu Glu Gln Pro Val Val Tyr Lys Gly Phe Leu Arg Tyr
545 550 555 560
Glu Phe Phe Lys Glu Asp Lys Lys Ser Phe Ala Arg Leu Val Glu Glu
565 570 575
Ala Ile Lys Thr Lys Trp Ser Asp Phe Asp Ile Pro Leu Gly Glu Glu
580 585 590
Tyr Tyr Asn Ile Pro Ser Leu Asp Arg Phe Asp Arg Thr Asn Lys Lys
595 600 605
Leu Tyr Glu Thr Leu Ala Met Asp Arg Leu Cys Leu Met Met Ala Arg
610 615 620
Gln Tyr Tyr Leu Arg Leu Asn Glu Lys Leu Ala Glu Lys Ala Gln His
625 630 635 640
Ile Tyr Trp Lys Lys Glu Asp Gly Arg Glu Val Ile Ile Phe Lys Phe
645 650 655
Gln Asn Pro Lys Glu Gln Lys Lys Ser Phe Ser Ile Arg Phe Ser Ile
660 665 670
Leu Asp Tyr Thr Lys Met Tyr Val Met Asp Asp Pro Glu Phe Leu Ser
675 680 685
Arg Leu Trp Glu Tyr Phe Ile Pro Lys Glu Ala Lys Glu Ile Asp Tyr
690 695 700
His Lys His Tyr Ala Arg Ala Phe Asp Lys Tyr Thr Asn Leu Gln Lys
705 710 715 720
Glu Gly Ile Asp Ala Ile Leu Lys Leu Glu Gly Arg Ile Ile Glu Arg
725 730 735
Arg Lys Ile Lys Pro Ala Lys Asn Tyr Ile Glu Phe Gln Glu Ile Met
740 745 750
Asn Arg Ser Gly Tyr Asn Asn Asp Gln Gln Val Ala Leu Lys Arg Val
755 760 765
Arg Asn Ala Leu Leu His Tyr Asn Leu Asn Phe Glu Arg Glu His Leu
770 775 780
Lys Arg Phe Tyr Gly Val Val Lys Arg Glu Gly Ile Glu Lys Lys Trp
785 790 795 800
Ser Leu Ile Val
<210> 15
<211> 799
<212> PRT
<213> 宏基因组(metagenomic)
<400> 15
Met Ile Asn Ile Glu Leu Lys Lys Glu Glu Ala Ala Phe Tyr Phe Asn
1 5 10 15
Gln Ala Asn Leu Asn Ile Ser Gly Leu Asp Glu Val Ile Glu Lys Gln
20 25 30
Leu Pro His Ile Gly Ser Lys Lys Glu Asn Ala Lys Lys Ala Ile Asp
35 40 45
Lys Ile Phe Asp Asn Ile Thr Val Leu Lys Lys Val Glu Asn Phe Val
50 55 60
Phe Tyr Phe Lys Asp Val Ala Lys Asn Glu Arg Val Glu Leu Asp Ala
65 70 75 80
Leu Leu Leu Lys Leu Ile Asp Leu Arg Asn Phe Tyr Ser His Tyr Val
85 90 95
His Asn Asp Asn Val Lys Ile Leu Ser Asp Gly Glu Glu Thr Leu Leu
100 105 110
Glu Lys Tyr Tyr Gln Ile Ala Ile Glu Ala Thr Gly Ser Lys Asp Val
115 120 125
Lys Leu Glu Ile Ile Asp Asn Glu Lys Lys Leu Thr Asp Ala Gly Ile
130 135 140
Leu Phe Leu Leu Cys Met Phe Leu Lys Lys Ser Gln Ala Asn Lys Leu
145 150 155 160
Ile Ser Ser Ile Ser Gly Phe Lys Arg Asn Asp Lys Glu Trp Gln Pro
165 170 175
Arg Arg Asn Leu Phe Thr Tyr Tyr Ser Leu Arg Glu Gly Tyr Lys Val
180 185 190
Val Pro Asp Met Glu Lys His Phe Leu Leu Phe Thr Leu Val Asn His
195 200 205
Leu Ser Thr Gln Asp Glu Asn Ile Glu Asn Thr Gln Pro Ser Asp Asp
210 215 220
Ile Gly Arg Gly Leu Phe Phe His Arg Ile Ala Ser Thr Phe Leu Asn
225 230 235 240
Ile Ser Gly Ile Phe Asn Asn Met Glu Phe Tyr Pro Tyr Gln Ser Asn
245 250 255
Arg Leu Lys Glu Arg Arg Gly Asp Ile Ala Pro Asp Lys Asp Ser Phe
260 265 270
Ala Trp Ile Glu Pro Phe Gln Gly Asn Ser Tyr Phe Lys Ile Asn Gly
275 280 285
Tyr Lys Gly Val Ile Gly Glu Asn Glu Leu Lys Glu Leu Cys Phe Ala
290 295 300
Val Leu Leu His Asn Lys Ser Lys Tyr Ala Val Glu Gln Ile Glu Lys
305 310 315 320
Phe Leu Lys Cys Phe Lys Glu Val Gln Ser Lys Gln Glu Ile Ile Glu
325 330 335
Cys Asp Ile Leu Asp Glu Cys Tyr Phe Pro Ala Asn Tyr Leu Asn Gln
340 345 350
Pro Glu Thr Lys Ser Leu Lys Glu Lys Leu Leu Ser Arg Ile Thr Gly
355 360 365
Lys Ile Asn Tyr Ser Phe Asp Thr Ala Glu Lys Ala Phe Asp Lys Met
370 375 380
Lys Asp Val Met Glu Phe Ile Asn Gly Cys Leu Pro Ser Asp Glu Lys
385 390 395 400
Leu Lys Arg Lys Asp Tyr Ser Arg Tyr Leu Lys Met Val Arg Phe Trp
405 410 415
Gly Gly Glu Lys Asp Asn Ile Lys Arg Glu Phe Glu Gly Lys Lys Trp
420 425 430
Thr Arg Phe Phe Pro Ser Glu Leu Trp His Lys Arg Thr Leu Glu Asp
435 440 445
Val Tyr Lys Phe Ala Leu Lys Lys Asn Lys Lys Arg Leu Glu Glu Leu
450 455 460
Lys Val Lys Ile Glu Gly Leu Asn Glu Asp Asp Leu Leu Lys Tyr Gln
465 470 475 480
Lys Val Asn Asn Ile Lys Asn Leu Glu Asn Leu Arg Leu Leu Ala His
485 490 495
Asp Leu Asp Leu Ser Trp Arg Glu Lys Asp Trp Gly Glu Tyr Ser Gly
500 505 510
Gln Ile Lys Lys Gln Ile Ser Asp Asn Gln Lys Leu Thr Ile Met Lys
515 520 525
Gln Arg Val Ile Ala Glu Leu Lys Lys Lys His Gly Ile Glu Asn Ile
530 535 540
Asn Leu Arg Ile Ser Leu Asp Ser Asn Lys Ser Ile Gln Ala Val Leu
545 550 555 560
Asn Arg Ile Ala Ile Pro Lys Gly Phe Ile Lys Arg His Val Leu His
565 570 575
Leu Gln Glu Asn Glu Lys Thr Ser Arg Lys Ile Arg Glu Ala Lys Cys
580 585 590
Lys Ile Leu Leu Ser Lys Lys Tyr Glu Tyr Leu Ser Arg Lys Phe Leu
595 600 605
Asp Glu Lys Asn Phe Asp Lys Leu Thr Gln Ile Asn Gly Leu Tyr Glu
610 615 620
Lys Asn Arg Leu Ile Ala Phe Met Val Ile Tyr Leu Leu Lys Gln Leu
625 630 635 640
Gly Leu Glu Leu Lys Asn Glu Thr Lys Leu Ile Glu Leu Lys Lys Thr
645 650 655
Arg Val Lys Tyr Lys Ile Ser Asp Lys Val Ala Glu Asp Ile Pro Leu
660 665 670
Ser His Tyr Pro Ser Leu Val Tyr Ala Met Ser Arg Lys Tyr Val Asp
675 680 685
Asn Ile Asp Asn Tyr Glu Phe Pro Asp Glu Tyr Ala Lys Lys Ala Ile
690 695 700
Leu Asp Lys Val Asp Ile Ile Glu Asn Gln Arg Met Glu Phe Ile Lys
705 710 715 720
Gln Val Leu Gly Phe Glu Lys Tyr Leu Phe Asp Asn Asn Ile Ile Asp
725 730 735
Lys Ser Lys Phe Thr Asp Val Glu Thr His Ile Ser Phe Val Lys Ile
740 745 750
His Asp Glu Leu Ile Glu Lys Gly Trp Asp Thr Glu Lys Leu Ser Lys
755 760 765
Leu Lys His Ala Arg Asn Lys Ala Leu His Gly Glu Ile Pro Gly Gly
770 775 780
Thr Ser Phe Glu Lys Ala Lys Leu Leu Ile Asn Glu Leu Lys Lys
785 790 795
<210> 16
<211> 650
<212> PRT
<213> 宏基因组(metagenomic)
<400> 16
Met Asp Thr Asn Glu Ala Tyr Thr Ala Tyr Asn Ser Arg Asn Ser Phe
1 5 10 15
Lys Arg Ile Phe Asp Phe Lys Gly Glu Ile Ala Pro Ile Ala Glu Lys
20 25 30
Ala Asn Leu Asn Tyr Asp Ile Lys Ala Lys Asn Ala Ile Asn Arg Glu
35 40 45
Gln Arg Leu His Tyr Phe Thr Val Gly His Thr Phe Lys Asn Ile Asp
50 55 60
Thr Glu His Val Phe Glu Ile Leu Leu Asp Glu Glu Thr Arg Glu Lys
65 70 75 80
Arg Pro Tyr Thr Phe Leu Ser Leu Gln Gln Phe Asn Thr Asp Phe Cys
85 90 95
Thr Ala Ile Lys Glu Val Ile Ser Asn Ile Arg His Ile Asn Ser His
100 105 110
Tyr Ile His Asp Phe Glu Arg Ile Lys Thr Asp Asn Ile Pro Pro Glu
115 120 125
Ile Ile Thr Phe Leu Lys Glu Ser Phe Glu Leu Ala Val Ile Gln Ile
130 135 140
Tyr Leu Lys Glu Asn Asn Ile Thr Tyr Leu Gln Phe Ile Glu Gln Lys
145 150 155 160
Asn Thr Asp Thr Thr Ile Val Lys Tyr Leu His Asp Lys Phe Tyr Ser
165 170 175
Leu Asp Asn Thr Lys Thr Asp Thr Lys Asn Asp Thr Ser Pro Ser Leu
180 185 190
Ala Glu Tyr Ile Ala Phe Arg Asn Thr Phe Lys Thr Leu Ser Lys Glu
195 200 205
Lys Ala Leu Asp Ser Leu Leu Phe Val Thr Val Asp Ala Gly Phe Pro
210 215 220
Trp Lys Leu Glu Glu Thr His Thr Ala Cys Thr Ile Thr Gln Gly Thr
225 230 235 240
Tyr Leu Ser Phe Asn Ala Cys Leu Phe Leu Leu Ser Leu Phe Leu Tyr
245 250 255
Lys Ser Glu Ala Asn Gln Leu Ile Ser Lys Ile Lys Gly Phe Lys Lys
260 265 270
Asn Lys Thr Asp Glu Glu Lys Ser Lys Arg Glu Ile Phe Ser Phe Phe
275 280 285
Ser Lys Lys Phe Ser Ser Gln Asp Ile Asp Ser Glu Glu Asn His Leu
290 295 300
Val Lys Phe Arg Asp Leu Ile Gln Tyr Ile Asn His Tyr Pro Val Glu
305 310 315 320
Trp Asn Lys Asp Leu Lys Leu Glu Ser Gly His Pro Leu Met Thr Asp
325 330 335
Lys Leu Ile Ala Lys Ile Thr Asp Met Glu Ile Asp Arg Ala Tyr Pro
340 345 350
Asp Tyr Ala Gly Asn Asn Lys Phe Gln Ala Tyr Ala Lys Glu Leu Leu
355 360 365
Trp Asn Val Pro Ser Lys Thr Thr Phe Thr Thr Glu Glu Ile Glu Ala
370 375 380
Phe Ala Phe Glu Ile Asn Lys Ser Pro Glu Leu Lys Asp Ala Lys Lys
385 390 395 400
Lys Leu His Asp Leu Gln Ala Lys Met Gly Leu Tyr Gly Phe Lys Lys
405 410 415
Val Lys Asn Glu Gln Glu Ile Ala Lys Thr Ile Lys Arg Ile Lys Trp
420 425 430
Ile Gln Asn Asp Leu Asn Pro Val Thr Glu Asn Val Lys Lys Arg Leu
435 440 445
Ala Gln Phe Ser Leu Tyr Gly Ser Tyr Gly Arg Asn Gln Asp Arg Phe
450 455 460
Met Asp Phe Ala Thr Arg Tyr Leu Ala Glu Gln Lys Tyr Phe Gly Val
465 470 475 480
Asp Ala Glu Phe Lys Met Tyr Lys Tyr Phe Thr Ser Glu Glu Gln Asn
485 490 495
Thr Glu Leu Ala Thr Tyr Glu Leu Pro Lys Asp Lys Lys Ala Tyr Asp
500 505 510
Lys Leu Arg Phe His Lys Gly Lys Leu Val His Phe Ser Thr Phe Glu
515 520 525
Asn His Leu Lys Lys Tyr Glu Ser Trp Asp Thr Pro Phe Val Ile Glu
530 535 540
Asn Asn Ala Ile Gln Val Lys Leu Ser Ile Arg Gln Asp Asn Lys Lys
545 550 555 560
Glu Pro Ile Glu Lys Ile Leu Ser Ile Gln Arg Ala Leu Met Leu Tyr
565 570 575
Phe Leu Glu Asp Ala Leu Phe Gln Thr Gly Asn Asn Asn Ile Ile Glu
580 585 590
Asn Lys Gly Arg Ile Leu Val Glu Gln Tyr Tyr Thr Val Tyr Asn Asn
595 600 605
Asp Phe Val Gln Ser Lys Thr Val Leu Glu Glu Asn Asp Ser Ile Ser
610 615 620
Pro Glu Gln Lys Asn Ala Leu Lys Lys Ile Val Pro Lys Arg Leu Leu
625 630 635 640
His Arg Tyr Phe Ala Arg Ser Asn Lys Leu
645 650
<210> 17
<211> 695
<212> PRT
<213> 宏基因组(metagenomic)
<400> 17
Leu Pro Lys Leu Leu Ala Leu Gln Leu Leu Thr Arg Thr Ala Glu Gln
1 5 10 15
Pro Ile Ile Asn Phe Ile Gln Thr Thr Asn Ala Lys Ile Phe Asn Phe
20 25 30
Gly Glu Leu Glu Lys Ile Lys Asp Arg Thr Asn Tyr Ile Pro Glu Val
35 40 45
Phe Thr Lys Arg Val Val Arg Glu Arg Glu Leu Ile Asn Asn Lys Gly
50 55 60
Gly Ile Thr Tyr Leu Ser Asn Lys Leu Glu Met Asn Ile Leu Asn Lys
65 70 75 80
Tyr His Leu Ser Ile Val Gln Leu Ala Ala Met Asp Lys Asp Ser Phe
85 90 95
Lys Lys Leu Thr Gly Lys Asn Lys Lys Asp Ile Glu Leu Leu Ser Gln
100 105 110
Ile Lys Tyr Gln Tyr Leu Leu Lys Gln Arg Arg Glu Glu Leu Lys Lys
115 120 125
His Leu Pro Thr Gly Leu Asn Leu Asp Met Leu Pro Lys Arg Val Leu
130 135 140
Asp Tyr Leu Met Gln Ser Asn Glu Arg Asn Glu Lys Lys Lys Ile His
145 150 155 160
Gln Lys Ile Lys Asp Ile Lys Asp Glu Ala Lys Leu Leu Asn Lys Ser
165 170 175
Ile Lys Lys Glu Ile Glu Lys Thr Gln Gln Glu Gln Arg Ile Lys Leu
180 185 190
Gly Glu Ile Ala Thr Phe Ile Ala Ser Asp Met Leu Asn Met Ile Ile
195 200 205
Asp Lys Glu Leu Lys Ser Arg Ile Thr Asn Pro Tyr Tyr Asn Lys Leu
210 215 220
Gln Asn Lys Ile Ala Tyr Phe Ser Leu Asn Lys Gly Glu Ile Ile Ser
225 230 235 240
Ile Cys Asp Glu Leu Ser Leu Phe Asp Thr Lys Lys Gly His Val Phe
245 250 255
Leu Thr Arg Asn Leu Ile Phe Lys Ser Thr Gly Ile Ile Asp Phe Tyr
260 265 270
Gln Asn Tyr Leu Glu Ala Lys Ile Ala Trp Ile Asp Asn Lys Leu Phe
275 280 285
Val Lys Gly Lys Met Gly Gly Tyr Thr Ile Pro Asn Asn Asn Val Pro
290 295 300
Tyr Ser Ile Leu Lys Ile Lys Asn Glu Ile Arg Thr Tyr Asn Phe Asn
305 310 315 320
Thr Trp Leu Lys Asn Lys Ile Ile Ser Pro Val Glu Leu Pro Asn Ser
325 330 335
Leu Met Asp Asp Gln Leu Ala Lys Val Leu Lys Ser Ala Leu Lys Lys
340 345 350
Glu Ser Lys Thr Tyr Asn Asp Gly Asp Lys Phe Ser Ile Leu Leu Ala
355 360 365
Lys Tyr Leu Ser Asn Asp Ser Gln Pro Phe Tyr Glu Tyr Thr Arg Asn
370 375 380
Tyr Thr Ile Asn Thr Glu Pro Val Cys Phe Asp Val Gln Gly Leu Ser
385 390 395 400
Ser Lys Asp Ile Gln Asp Lys Tyr Gly Asn Arg Val Ala Glu Asn Glu
405 410 415
Lys Leu Ile Arg Phe Ile Gln Thr Lys Asp Arg Ile Leu Lys Leu Val
420 425 430
Cys Asp Glu Leu Leu Leu Lys Asp Pro Ser Ile Gly Ser Lys Asp Arg
435 440 445
Phe Lys Leu Ser Glu Ile His Pro Thr Ser Leu His Asn Pro Leu Glu
450 455 460
Arg Gln Ala Glu Phe Lys His Lys Ile Ile Arg Lys Gly Thr Glu Val
465 470 475 480
Phe Phe Thr Val Val Ala Lys Asp Thr Lys Glu Gln Ile Gln Glu Val
485 490 495
Ile Glu Tyr Glu Lys Ile Thr Ser Ser Asp Glu Lys Glu Lys Tyr Pro
500 505 510
Gly Gln Lys Trp Tyr Gln Trp Thr Ile Lys Asp Phe Gly Arg Phe Lys
515 520 525
Arg Phe Leu Lys Asp Arg Arg Leu Pro Asn Leu Ser Glu Tyr Phe Glu
530 535 540
Asp Lys Asp Ile Thr Phe Asp Phe Leu Glu Tyr Gln Ile Lys Glu Tyr
545 550 555 560
Asp Lys Tyr Arg Asn Asn Val Phe Asp Leu Thr Phe Glu Leu Glu Lys
565 570 575
Ser Ile Ala Thr Ser Asp Leu Glu Gly Ile Arg Gln Ile Glu Gln Arg
580 585 590
Leu His Ile Arg Pro Lys Gly Phe Phe Glu Ile Glu Phe Asp Val Tyr
595 600 605
Val Glu Trp Leu Asp Arg Lys Gly Ile Gln Tyr Asn Lys Asp Leu Ile
610 615 620
Asn Glu Cys Arg Asn Arg Phe Ser His Ser Glu Phe Pro Lys Tyr Glu
625 630 635 640
His Leu Asn Gln Ile Pro Lys Ile Thr Arg Gln Gln Ile Ile Asp Phe
645 650 655
Glu Tyr Asn Lys Arg Thr Arg Glu Tyr Lys Asn Asn Ala Asp Ile Ser
660 665 670
Ile Ser Glu Lys Ile Thr Asn Gln Tyr Lys Thr Glu Ile Glu Gln Ile
675 680 685
Ile His Gln Ile Arg Leu Gln
690 695
<210> 18
<211> 436
<212> PRT
<213> 宏基因组(metagenomic)
<400> 18
Met Ala His Asp Lys Ile Ile Arg Leu Tyr Arg Asn Thr Leu Cys Val
1 5 10 15
Tyr Ser Asn Ile Ala Ala Asp Asn Ile Asp Lys Trp Leu His Lys Gln
20 25 30
Lys Pro Ser Lys Lys Ala Thr Phe Glu Glu Gln Thr Lys Ile Val Asn
35 40 45
Ala Ala Tyr Lys Asn Ile Tyr Phe Val Glu Trp Ala Ile Asn Asn Gly
50 55 60
Val Phe Phe Leu Pro Glu Ser Asn Asp Ser Ser Glu Gln Glu Lys Ala
65 70 75 80
Ile Lys Glu Asn Tyr Cys Arg Leu Leu Gly Val Ile Gln Leu Leu Asp
85 90 95
Asn Val Arg Asn Tyr Trp Ser His Leu Lys His Asn Lys Thr Ile Ile
100 105 110
Ala Ala Asn Leu Asp Gly Lys Asn Asn Glu Thr Val Lys Asn Lys Ser
115 120 125
Glu Asn Ser Asn Asn Glu Asp Thr Cys Asn Ala Val Leu Ser Gly Leu
130 135 140
Lys Asp Phe Ile Thr Gln Ala Tyr Val Ala Ala Cys Ser Glu Thr Gly
145 150 155 160
Val Lys Ile Pro Asp Lys Tyr Lys Ala Ser Glu Gly Leu Phe Ala Cys
165 170 175
Val Lys Lys Pro Glu Glu Asp Tyr Lys Val Ile Lys Ile Thr Ser Glu
180 185 190
Phe Thr Ile Thr Gly Phe Val Phe Tyr Ile Cys Leu Phe Leu Asp Gly
195 200 205
Gln Gln Ile Asn Glu Phe Leu Glu Ala Met Glu Gln Ser Val Tyr Asn
210 215 220
Phe Asp Glu Leu Ala Asp Arg Gln Asn Phe Arg Leu Thr His Pro Lys
225 230 235 240
Glu Lys Tyr Pro Glu Ser Leu Ser Lys Lys Ile Lys Asp Phe Phe Tyr
245 250 255
Ala Arg Asp Val Tyr Thr Tyr Trp Arg Val Arg Gly His Arg Asp Asn
260 265 270
Ile Leu Glu Asp Lys Thr Leu Asp Glu Lys Glu Asp Tyr Tyr Ser Leu
275 280 285
Leu Glu Tyr Leu Lys Arg Cys Pro Lys Glu Arg Leu Glu Leu Val Glu
290 295 300
Glu Ser Pro Ser Gly Lys Lys Glu Phe Val Ile Asp Asn Val Ser Tyr
305 310 315 320
Asp Val Arg Glu Lys Asn Lys Phe Met Glu Trp Gly Leu Glu Phe Trp
325 330 335
Asp Asn Glu Ile Glu Arg Leu Met Lys Ile Tyr Pro Asp Ser Lys Leu
340 345 350
Asp Met Trp Lys Trp Gly His His Gln Thr Ala Asp Tyr Lys His Asn
355 360 365
Ile Lys Leu Glu Leu Lys Gln Gln Ala Glu Glu Gln Gly Arg Pro Tyr
370 375 380
Gln Phe Pro Arg Tyr Glu Lys Val Ile Phe Asp Leu Pro Glu Thr Glu
385 390 395 400
Glu Glu Arg Arg Asn Tyr Arg Asn Asp Glu His Gly Phe Thr Tyr Tyr
405 410 415
Leu Leu Lys Asp Asp Ser Asn Thr Phe Ser Gln Ala Met Phe Ser Tyr
420 425 430
Gln Phe Leu Cys
435
<210> 19
<211> 866
<212> PRT
<213> 宏基因组(metagenomic)
<400> 19
Met Ala Val Asp Tyr Ser Leu Lys Asn Glu Trp Tyr Arg Glu Ile Asn
1 5 10 15
Lys Ser Cys Phe Thr Val Ala Leu Asn Val Ala Tyr Asp Asn Cys Lys
20 25 30
Ala Lys Gly His Glu Asn Leu Leu Arg Glu Ala Gln Arg Ser Lys Gly
35 40 45
Gly Ile Thr Asn Glu Gln Ile Lys Asn Val Gln Thr Glu Ile Lys Thr
50 55 60
Arg Leu Glu Asp Ile Arg Ser His Phe Ser His Phe Tyr His Asp Glu
65 70 75 80
Lys Ser Leu Ile Phe Glu Lys Asp Asn Ile Val Lys Asp Phe Leu Glu
85 90 95
Ser Ala Tyr Glu Lys Ala Gln Ser Ser Val Ile Gly Ser Thr Arg Gln
100 105 110
Ser Asp Tyr Lys Gly Val Val Pro Pro Leu Phe Glu Pro His Asp Gly
115 120 125
Met Ile Thr Ala Ala Gly Val Val Phe Leu Ala Ser Phe Phe Cys His
130 135 140
Arg Ser Asn Val Tyr Arg Met Leu Gly Ala Val Lys Gly Phe Lys His
145 150 155 160
Thr Gly Lys Glu Glu Leu Ser Asp Gly Ala Lys Arg Asp Tyr Gly Phe
165 170 175
Thr Arg Arg Leu Met Ala His Tyr Ser Leu Arg Asp Ser Tyr Val Ile
180 185 190
Lys Ala Glu Glu Thr Lys Ser Phe Arg Asp Leu Leu Gly Tyr Leu Ser
195 200 205
Arg Val Pro Gln Gln Ala Val Asp Trp Leu Asn Glu His Asn Gln Leu
210 215 220
Ser Glu Asp Glu Lys Lys Glu Phe Leu Asn Gln Lys Pro Ser Asp Glu
225 230 235 240
Glu Ser Gln Glu Gln Ser Lys Thr Glu Asn Thr Asp Arg Gln Ala Asp
245 250 255
Arg Met Pro Arg Arg Ser Leu Arg Lys Thr Asp Lys Phe Ile Leu Phe
260 265 270
Ala Ala Lys Phe Ile Glu Asp Trp Ala Gln Lys Glu Lys Met Asp Val
275 280 285
Thr Phe Ala Arg Tyr Gln Lys Thr Val Thr Glu Asp Glu Asn Lys Asn
290 295 300
Gln Asp Gly Lys Gln Val Arg Asp Val Gln Leu Lys Tyr Glu Lys Asp
305 310 315 320
Thr Lys Lys Leu Asn Pro Asp Phe Asp Tyr Lys Trp Thr Tyr Tyr Ile
325 330 335
Arg Asn Asn His Ala Ile Ile Gln Ile Lys Pro Asp Glu Tyr Lys Gln
340 345 350
Ala Val Ser Ala Arg Ile Ser Glu Asn Glu Leu Lys Tyr Leu Val Leu
355 360 365
Leu Ile Phe Gln Gly Lys Gly Trp Glu Ala Ile Lys Lys Ile Gly Asp
370 375 380
Tyr Ile Phe His Ile Gly Asn Lys Ile Lys Ile Gly Arg Phe Asp His
385 390 395 400
Asn Glu Glu Arg Arg Met Pro Ser Phe Leu Lys Asn Pro Pro Ala Asp
405 410 415
Ile Ile Gly Glu Met Val Glu Asn Arg Leu Lys Tyr Ile Arg Asp Glu
420 425 430
Leu Asn Lys Val Ile Glu Thr Ile Lys Lys Glu Glu Pro Gln Asn Asn
435 440 445
Lys Trp Leu Leu Tyr Lys Gly Lys Lys Ile Ser Ile Ile Leu Lys Phe
450 455 460
Ile Ser Asp Ser Ile Ser Asp Ile Lys Lys Arg Pro Asp Val Asn Glu
465 470 475 480
Tyr Asn Thr Leu Arg Asp Met Leu Gln Lys Leu Asp Phe Asp Asn Phe
485 490 495
Tyr Glu Arg Leu Lys Ser Tyr Val Ser Glu Gly Arg Ile Glu Gln Thr
500 505 510
Leu Tyr Asp Glu Ile Lys Gly Ile Lys Asp Ile Ser Thr Leu Cys Ile
515 520 525
Lys Ile Cys Glu Leu Arg Leu Ala Ala Leu Glu Glu Leu Glu Lys Glu
530 535 540
Gly Gly Asp Asp Leu Asn Lys Tyr Ile Gly Leu Ala Val Gln Glu Lys
545 550 555 560
His Lys Asn Tyr Asp Asp Ser Asn Thr Pro Gln Lys Lys Ala Glu Arg
565 570 575
Phe Leu Glu Ser Gln Phe Ser Val Gly Lys Asn Phe Leu Arg Glu Thr
580 585 590
Phe Tyr Asp Glu Tyr Ile Lys Asn Arg Lys Ser Leu Tyr Glu Ile Ile
595 600 605
Lys Glu Lys Ile Thr Gly Ile Thr Pro Leu Asn Glu Asn Arg Trp Tyr
610 615 620
Leu Met Asp Lys Asn Pro Lys Glu Phe Glu Ser Lys Asp Ser Lys Ile
625 630 635 640
Ile Arg Gly Leu Cys Asn Ile Tyr Ile Gln Asp Ile Leu Cys Met Lys
645 650 655
Ile Ala Leu Trp Tyr Tyr Glu Asn Leu Ser Pro Ser Tyr Lys Asn Lys
660 665 670
Leu Lys Trp Asp Phe Ile Gly Gln Gly Phe Gly Tyr Asp Arg Tyr Lys
675 680 685
Leu Ser Tyr Lys Thr Asp Cys Gly Ile Thr Ile Glu Phe Lys Leu Ala
690 695 700
Asp Leu Asn Arg Leu Asp Ile Ile Glu Lys Pro Lys Met Ile Glu Asn
705 710 715 720
Ile Cys His Ser Phe Ile Leu Glu Lys Asp Val Lys Lys Gln Thr Ile
725 730 735
Ser Trp His Glu Phe Arg Gln Asp Gly Ile Ala Lys Tyr Arg Lys Leu
740 745 750
Gln Lys Glu Val Val Glu Ala Val Phe Glu Phe Glu Asn Ser Leu Lys
755 760 765
Ile Pro Asp Lys Asn Trp Leu Thr Gln Gly Tyr Val Pro Phe Asn Lys
770 775 780
Asn Lys Arg Phe Glu Asp Lys Gly Phe Ser Thr Phe Ile Leu Glu Glu
785 790 795 800
Ala Val Arg Lys Gly Lys Ile Lys Ser Asp Asp Lys Glu Pro Leu Arg
805 810 815
Lys Val Arg Thr Asp Phe Phe His Glu Gln Phe Asp Ser Thr Asp Ala
820 825 830
Glu Arg Arg Ile Phe Asp Lys Tyr Met Pro Ala Lys His Asp Gly Lys
835 840 845
Asn Lys Gly Gly Lys Met Gln Glu Lys Gln Glu Lys Ser Tyr Thr Arg
850 855 860
Arg Ile
865
<210> 20
<211> 791
<212> PRT
<213> 宏基因组(metagenomic)
<400> 20
Met Asp Thr Ile Glu Leu Lys Lys Glu Glu Ala Ala Phe Tyr Phe Asn
1 5 10 15
Gln Ala Gly Phe Asn Leu Arg Ala Ile Glu Ser Asn Val Phe Asp Ala
20 25 30
Gly Lys Arg Lys Thr Leu Leu Glu Asn Pro Gln Val Leu Ala Lys Leu
35 40 45
Glu Asn Phe Ile Phe Asn Phe Arg Asp Val Thr Lys Asn Glu Lys Gly
50 55 60
Glu Ile Asp Val Leu Ile Ser Lys Leu Thr Asp Leu Arg Asn Tyr Tyr
65 70 75 80
Ser His Tyr Val His Thr Asp Asn Val Lys Val Leu Ser Lys Gly Glu
85 90 95
Gly Pro Ile Leu Ala Arg Tyr Tyr Gln Phe Ala Thr Glu Ala Thr Ala
100 105 110
Ser Thr Asn Val Lys Leu Glu Ile Met Asp Lys Gly Asn Lys Leu Thr
115 120 125
Asp Ala Gly Val Leu Phe Phe Leu Cys Met Phe Leu Lys Lys Ser Gln
130 135 140
Ala Asn Lys Leu Ile Ser Ser Ile Ser Gly Phe Lys Arg Thr Asp Ala
145 150 155 160
Glu Gly Gln Pro Arg Arg Asn Leu Phe Thr Tyr Phe Ser Val Arg Glu
165 170 175
Gly Tyr Lys Val Val Pro Glu Met Gln Lys His Phe Leu Leu Phe Asp
180 185 190
Leu Val Asn His Leu Ser Asn Gln Asp Glu Tyr Ile Glu Lys Ser Gln
195 200 205
Gln Thr Tyr Asp Ile Gly Glu Gly Leu Phe Phe His Arg Met Ala Ser
210 215 220
Lys Phe Leu Asn Thr Ser Gly Ile Leu Arg Gly Met Lys Phe Tyr Ala
225 230 235 240
Tyr Gln Ser Lys Arg Leu Glu Glu Lys Arg Gly Lys Ile Glu Pro Glu
245 250 255
Gly Asp Ser Phe Val Trp Ile Glu Pro Phe Gln Gly Asn Ser Tyr Phe
260 265 270
Glu Val Asp Gly His Lys Gly Val Ile Gly Glu Asp Glu Leu Lys Asp
275 280 285
Leu Cys Tyr Ala Leu Leu Val Ala Gly Lys Asn Ala Asn Glu Ala Glu
290 295 300
Gly Lys Ile Thr Gln Phe Leu Thr Lys Tyr Lys Lys Ala Asp Asn Ser
305 310 315 320
Gln Glu Ile Glu Lys Asp Glu Met Leu Cys Ile Asp Asn Phe Pro Ala
325 330 335
Asn Tyr Phe Asp Gly Pro Gly Val Gly Ser Ile Lys Asp Arg Val Leu
340 345 350
Asn Arg Leu Glu Arg Glu Ile Lys Ser His Lys Asp Asn Lys Ala Asp
355 360 365
Ser Lys Ala Tyr Asp Lys Met Lys Glu Val Met Asp Phe Ile Asn Asn
370 375 380
Arg Leu Pro Ala Ala Glu Lys Met Lys Gln Lys Asp Tyr Arg Arg Tyr
385 390 395 400
Leu Lys Met Val Arg Leu Trp Asn Arg Glu Lys Gly Asn Ile Glu Arg
405 410 415
Glu Phe Lys Ala Lys Glu Trp Ser Lys Tyr Phe Pro Ser Asn Phe Trp
420 425 430
Arg Ala Asn Asn Leu Glu Asp Val Tyr Lys Leu Ala Arg Gln Glu Asn
435 440 445
Ala Arg Ile Leu Gly Asn Leu Lys Ala Val Val Glu Gly Met Ser Glu
450 455 460
Gln Glu Phe Glu Lys Tyr Arg Gln Ile Asn Glu Ala Lys Asp Leu Ala
465 470 475 480
Gly Leu Arg Gln Leu Ala Gly Ser Phe Gly Val Lys Trp Glu Glu Lys
485 490 495
Asp Trp Glu Glu Tyr Ala Arg Gln Ile Lys Glu Arg Ile Thr Asp Arg
500 505 510
Gln Lys Leu Thr Ile Met Lys Gln Arg Ile Thr Ala Glu Leu Lys Lys
515 520 525
Arg His Ala Ile Glu Asn Leu Asn Leu Arg Ile Thr Ile Asp Ser Ser
530 535 540
Lys Ser Arg Ala Ala Val Leu Asn Arg Ile Ala Leu Pro Lys Gly Phe
545 550 555 560
Val Lys Thr His Ile Leu Gln Thr Pro Ser Asp Lys Ile Met Lys Lys
565 570 575
Thr Arg Glu Ala Glu Cys Lys Ile Leu Leu Ser Gly Lys Tyr Gly Asp
580 585 590
Leu Ser Lys Arg Phe Phe Asp Glu Lys Asn Leu Asp Lys Leu Thr Gln
595 600 605
Ile Asn Gly Leu Tyr Glu Lys Asn Lys Ile Ile Ala Phe Met Val Val
610 615 620
Tyr Leu Met Glu Gln Leu Lys Leu Gly Leu Lys Gly Lys Thr Lys Leu
625 630 635 640
Ala Glu Leu Lys Glu Thr Arg Ile Arg Tyr Lys Ile Ser Asp Lys Val
645 650 655
Thr Glu Asp Ile Gln Leu Ser Gln Tyr Pro Ser Leu Val Tyr Val Ile
660 665 670
Gly Arg Lys Tyr Ile Asp Asn Val Asp Arg Tyr Lys Phe Ala Gly Asp
675 680 685
Val Arg Gly Lys Pro Ile Leu Ser Lys Ile Asp Leu Ile Glu Lys Glu
690 695 700
Arg Leu Glu Phe Ile Arg Gln Val Leu Gly Phe Glu Lys Glu Leu Phe
705 710 715 720
Asp Asn Thr Val Ile Asp Lys Lys Asn Phe Thr Asp Thr Glu Thr His
725 730 735
Ile Lys Phe Arg Gln Ile Ile Asp Glu Leu Val Arg Lys Gly Trp Asp
740 745 750
Ala Thr Lys Leu Asn Lys Leu Lys Glu Ala Arg Asn Ala Ala Leu His
755 760 765
Gly Glu Ile Pro Glu Gly Thr Ser Phe Gln Glu Ala Asn Val Leu Ile
770 775 780
Asn Glu Leu Lys Asn Lys Lys
785 790
<210> 21
<211> 767
<212> PRT
<213> 宏基因组(metagenomic)
<400> 21
Leu Tyr Gly Phe Asp Lys Leu Gln Lys Phe Asn Glu Lys Asn Gln Pro
1 5 10 15
Glu Glu Trp Lys Asn Leu Ile Lys Asp Leu Asp Tyr Phe Glu Ser Ser
20 25 30
Asp Leu Pro Phe Val Ser Lys Thr Thr Pro His Tyr His Phe Ile Ala
35 40 45
Asn Lys Ile Gly Ile Lys Phe Val Tyr Arg Asn Asn Lys Lys Trp Pro
50 55 60
Ser Leu Glu Val Lys Glu Lys Ser Glu Gly Lys Ile Lys Tyr Asn Tyr
65 70 75 80
Asp Lys Gln Phe Val Ala Asp Ala Phe Ile Ser Val His Glu Leu Val
85 90 95
Pro Met Met Phe Tyr Tyr Leu Leu Thr Lys Asn Gly Asn Ser Ile Glu
100 105 110
Lys Ile Ile Lys Gln Thr Ile Asp Asp Val Tyr Asn Ile Tyr Asp Glu
115 120 125
Phe Lys Asp Asn Lys Ile Asn Asp Leu Asp Asp Leu Val Gln Lys Ile
130 135 140
Ser Lys Ser Asn Ile Leu Ile Gly His Leu Pro Lys Gln Met Ile Ser
145 150 155 160
Ile Leu Lys Lys Thr Gln Lys Asn Met Gln Asn Glu Ala Ser Ile Lys
165 170 175
Leu Ile Lys Leu Ile Glu Asp Thr Glu Lys Arg Lys Asn Lys Leu Glu
180 185 190
Asp Gln Ile Lys Gln Lys Ile Arg Ile Gly Lys Arg Asn Ser Gly Leu
195 200 205
Leu Lys Ser Gly Ile Ile Ala Asp Trp Leu Ile Lys Asp Leu Met Arg
210 215 220
Phe Gln Pro Val Ala Leu Asp Ser Asn Lys Asn Pro Leu Asn Arg Ser
225 230 235 240
Lys Ala Asn Ser Thr Glu Tyr Gln Leu Leu Gln Lys Thr Phe Ala Leu
245 250 255
Tyr Gly Gly Glu Lys Asp Arg Leu Pro Ala Tyr Phe Lys Gln Leu Asn
260 265 270
Leu Ile His Ser Asp Asn Pro His Pro Phe Leu Asn Ala Phe Asp Trp
275 280 285
Asp Asn Gln Pro Asn Ile Leu Ser Phe Tyr Glu Lys Tyr Leu Asn Ala
290 295 300
Lys Leu Asp Tyr Leu Lys Gly Leu Ser Pro Lys Asn Trp Gln Lys His
305 310 315 320
Gln His Phe Leu Leu Leu Lys Val Pro Lys Thr Asp Arg Asn Thr Leu
325 330 335
Val Ala Gly Trp Lys Asn Tyr Phe Asn Leu Pro Arg Gly Leu Phe Thr
340 345 350
Gln Ala Ile Lys Asp Trp Ile Ile Glu His Glu Glu Ser Pro Leu Lys
355 360 365
Lys Lys Ile Asn Ile Phe Asp Arg Pro Gly Phe Ile Val Lys Thr Ile
370 375 380
Pro Leu Tyr Phe Arg Glu Lys Asn Asn Asp Asp Val Pro Lys His Tyr
385 390 395 400
Gln Tyr Asp Cys Asn Val Ala Asn Pro Leu Lys Leu Lys Ala Ala Asn
405 410 415
Phe Ile Ser Ile Glu Asn Arg Asn Glu Tyr Leu Ile Lys Asn Lys Lys
420 425 430
Val Leu Asn Leu Gln Lys Thr Asn Asp Gln Ile Ile Lys Ile Asn Ser
435 440 445
Val Phe Asp Ser Val Glu Asn Asn Asp Arg Ser Trp Val Glu Lys Ile
450 455 460
Val Glu Leu Lys Lys Leu Met His Glu Leu Glu Leu Pro Lys Lys Leu
465 470 475 480
Lys Asp Lys Leu Glu Asn Arg Ile Asn Leu Lys Ser Leu Lys Glu Glu
485 490 495
Val Ile Ser Ala Ala Lys Lys Pro Tyr Leu Asp Phe Lys Lys Trp Arg
500 505 510
Lys Phe Glu Lys Glu Met Arg Leu Phe Lys Asn Gln Asp Ile Leu Met
515 520 525
Trp Leu Met Cys Lys Asp Leu Gln Thr Asp Lys Gln Met Thr Ser Leu
530 535 540
Ala Ile Asn Asp Leu Tyr Leu Lys Asn Ile Asn Thr Asp Val His Ser
545 550 555 560
Val Gly Ser Leu Asn Ile Leu Asn Arg Val Leu Pro Met Lys Leu Ser
565 570 575
Ile Asp Val Phe Pro Thr Asp Asp Lys Gly Asn Val Leu Arg Asn Lys
580 585 590
Asp Ser Ile Asn Lys Val Tyr Ile Glu Glu Lys Gln Thr Lys Val Leu
595 600 605
Lys Gln Gly Asn Phe Lys Ala Leu Met Lys Asp Arg Arg Leu Asn Cys
610 615 620
Leu Phe Ser Phe Ile Glu Ser Glu Gly Val Asp Met Glu Asn Asn Pro
625 630 635 640
Ile Ser Lys Leu Arg Leu Glu Tyr Glu Leu Ala Lys Tyr Gln Thr Ser
645 650 655
Arg Ile Asn Ile Phe Glu Ile Thr Leu Asp Ile Glu Lys Asn Leu Ile
660 665 670
Ser Lys His Pro Asn Leu Pro Ser Tyr Asn Phe Arg Glu Met Ile Glu
675 680 685
Val Trp Ile Lys Gln Lys Asp Asn Arg Asn Glu Leu Glu His Phe Thr
690 695 700
Lys Pro Leu Ile Ala Ile Arg Asn Ala Phe Ser His Asn Gln Tyr Pro
705 710 715 720
Met Tyr Asn Lys Thr Leu Phe Lys Asp Ile Arg Leu Phe Asn Ile Ile
725 730 735
Glu Pro Asn Ile Asp Glu Ser Gln Gly Leu Asn Ile Ala Phe Gln Leu
740 745 750
Thr Glu Leu Thr Lys Glu Leu Lys Thr Ile Ile Asn Lys Asn Ile
755 760 765
<210> 22
<211> 598
<212> PRT
<213> 宏基因组(metagenomic)
<400> 22
Met Gly Phe Ser Ile Lys Lys Leu Arg Glu Ile Ile Leu Glu Gly Thr
1 5 10 15
Glu Tyr Asn Asp Asn Lys Tyr Asp Thr Val Arg Asn Lys Leu Tyr Gln
20 25 30
Ile Val Asp Phe Ile Leu Tyr Arg Gly Tyr Ile Asn Glu Asn Ser Glu
35 40 45
Arg Ala Glu Val Leu Val Asn Ala Leu Arg Ser Thr Leu Asn Glu Asp
50 55 60
Asp Lys Thr Lys Leu Tyr Ser Ser Glu Ala Ala Phe Leu Lys Lys Lys
65 70 75 80
Tyr Met Lys Ile Ile Arg Lys Ala Ala Asp Ser Leu Asp Val Lys Lys
85 90 95
Leu Lys Asp Leu Lys Lys Lys Ala Phe Thr Ile Pro Asp Asn Glu Leu
100 105 110
Arg Lys Cys Phe Ile Ser Tyr Ala Asp Ser Val Ser Glu Phe Thr Lys
115 120 125
Leu Ile Tyr Leu Leu Thr Arg Phe Leu Ser Gly Lys Glu Ile Asn Asp
130 135 140
Leu Val Thr Thr Leu Ile Asn Lys Phe Asp Asn Ile Arg Ser Phe Leu
145 150 155 160
Glu Ile Met Asp Glu Leu Gly Leu Glu Arg Thr Phe Thr Asp Glu Tyr
165 170 175
Arg Phe Phe Glu Gly Ser Thr Lys Tyr Leu Ala Glu Leu Val Glu Leu
180 185 190
Asn Ser Phe Val Lys Ser Cys Ser Phe Asp Met Ser Ala Lys Arg Thr
195 200 205
Met Tyr Arg Asp Ala Leu Asp Ile Leu Gly Ile Glu Ser Asp Lys Thr
210 215 220
Glu Glu Asp Ile Glu Lys Met Ile Asp Asn Ile Leu Gln Val Asp Ala
225 230 235 240
Asn Gly Lys Lys Leu Pro Asn Lys Asn His Gly Leu Arg Asn Phe Ile
245 250 255
Ala Ser Asn Val Ile Asp Ser Asn Arg Phe Glu Tyr Leu Val Arg Tyr
260 265 270
Gly Asn Pro Lys Lys Ile Arg Glu Thr Ala Lys Cys Glu Pro Ala Val
275 280 285
Arg Phe Val Leu Asn Glu Ile Pro Asp Ala Gln Ile Glu Arg Tyr Tyr
290 295 300
Lys Ala Tyr Tyr Pro Asp Glu Lys Ser Leu Cys Leu Ala Asn Met Gln
305 310 315 320
Arg Asp Lys Leu Ala Gly Val Ile Ala Asp Ile Lys Phe Asp Asp Phe
325 330 335
Ser Asp Ala Gly Ser Tyr Gln Lys Ala Asn Ala Thr Ser Thr Arg Arg
340 345 350
Thr Ser Glu Ala Glu Ile Lys Arg Lys Asn Gln Ala Ile Ile Arg Leu
355 360 365
Tyr Leu Thr Val Met Tyr Ile Met Leu Lys Asn Leu Val Asn Val Asn
370 375 380
Ala Arg Tyr Val Ile Ala Phe His Cys Leu Glu Arg Asp Ala Lys Leu
385 390 395 400
Tyr Ser Glu Ser Val Leu Lys Val Gly Asn Thr Asn Glu Glu Ser Arg
405 410 415
Leu Gln Thr Gly Asn Thr Asn Glu Glu Lys Asn Lys Val Lys Leu Thr
420 425 430
Asn Leu Thr Asn Leu Thr Met Ala Val Met Gly Val Lys Leu Glu Asn
435 440 445
Gly Thr Ile Lys Thr Glu Phe Asp Lys Ser Leu Ala Glu Asn Ala Ala
450 455 460
Asn Arg Tyr Leu Arg Asn Ala Arg Trp Tyr Lys Leu Ile Leu Asp Asn
465 470 475 480
Leu Lys Lys Ser Glu Arg Ala Val Val Thr Glu Phe Arg Asn Thr Val
485 490 495
Cys His Leu Asn Ala Ile Arg Asn Ile Asn Ile Asn Ile Lys Glu Val
500 505 510
Lys Glu Val Glu Asn Tyr Phe Ala Leu Tyr His Tyr Leu Ile Gln Lys
515 520 525
His Leu Glu Asn Arg Phe Ala Asp Lys Lys Val Glu Arg Asp Thr Gly
530 535 540
Asp Phe Ile Ser Lys Leu Glu Glu His Lys Thr Tyr Cys Lys Asp Phe
545 550 555 560
Val Lys Ala Tyr Cys Thr Pro Phe Gly Tyr Asn Leu Val Arg Tyr Lys
565 570 575
Asn Leu Thr Ile Asp Gly Leu Phe Asp Lys Asn Tyr Pro Gly Lys Asp
580 585 590
Asp Ser Asp Lys Gln Lys
595
<210> 23
<211> 699
<212> PRT
<213> 宏基因组(metagenomic)
<400> 23
Met Asn His Gln Thr Arg Lys Ser Thr Ser Lys Arg Leu Gly Val Gln
1 5 10 15
Thr Ile Val Asn Lys Asn Asn Asn Ile Phe Val Phe Ser Asn Asn Gln
20 25 30
Pro Lys Thr Lys Ser Val Gln Leu Ala Asp Glu His Gln Gln Leu Ile
35 40 45
Glu Gln Asn Asn Asp Leu Asp Asn Phe Lys Thr Asp Leu Glu Tyr Gly
50 55 60
Asn Ile Lys Phe Gly Leu Ser Asn Asn Ala Leu Glu Ala Phe Tyr Pro
65 70 75 80
Asn Gln Tyr Thr Ile Arg Lys Lys Ser Ser Ser Ile Asn Gln Trp Leu
85 90 95
Val Gln Lys Tyr Cys Pro Thr Gln Cys Pro Asp Ser Asn Leu Ile Leu
100 105 110
Gln Leu Ile His Asn Asn Glu Asp Phe Lys Lys Ile Ile Ala Leu Tyr
115 120 125
Ser Asp Val Val Phe Ser Met Lys Val Phe Lys Gly Asn Arg Asn Glu
130 135 140
Asp Tyr Ile Ser Lys Ile Phe Asn Asn Met Asn Ala Lys Glu Ile Ile
145 150 155 160
Asp Lys Val Lys Ser Val Ile Asp Ser Ile Lys Lys Arg Pro Tyr Leu
165 170 175
Phe Gly Phe Asn Pro Lys Asp Ser Ala Ser Asp Gln Gln Ile Ala Tyr
180 185 190
His Leu Lys Asn Ile Ser Met Leu Arg Asn Tyr Ile Thr His Asp Ser
195 200 205
Asn Asp Asn Leu Arg Ile Asp Ser Asp Thr Tyr Ala Ser Leu Thr Glu
210 215 220
Gln Leu Asp Gln Phe Ile Glu Gln Glu Asn Lys Lys Phe Thr Glu Gln
225 230 235 240
Asn Gly His Asn Leu Ala Leu Ile Ser Gln Phe Tyr Pro Ser Trp Ser
245 250 255
Asp Glu Glu Val Val Lys Asn Tyr His Asp Tyr Tyr Phe Asn Met Leu
260 265 270
Asp Lys Asn Leu Gly Val Asn Leu Arg Gln Ile Ala Leu Leu Ile Arg
275 280 285
Glu Lys Asn Asn Ile Ser Gln Met Leu Asp Asp Asp Arg Arg Ala Lys
290 295 300
Phe Asn Val Val Leu Lys Phe Tyr Leu Tyr Thr Trp Leu Lys Ala Asn
305 310 315 320
Gln Ala Gln Leu Asp Asp Tyr Ile Gln Gln Leu Arg Gln Ala Gln Leu
325 330 335
Asp Asp Lys Asp Thr Ile Tyr Gln Lys Ile Ala Gln Asp Ile Pro Ala
340 345 350
Asn Asp Ile Asn Arg Leu Ile Lys Leu Cys Gln Gln Ile Asp Phe Gln
355 360 365
Ile Lys Pro Thr Arg Phe Lys Arg Ile Pro Ile Lys Thr Ile Asn Glu
370 375 380
Gln Trp Asp Phe Arg Val Leu Met Ile Tyr Ser Leu Thr Lys Phe Leu
385 390 395 400
Thr Ile Lys Glu Thr Asn Glu Leu Ile Ser Ala Leu Leu Asn Lys Ile
405 410 415
Glu Ala Met Ser Asn Leu Leu Glu Ile Asp Arg Ala Leu Lys Val Gln
420 425 430
Ala Val Asp Ala Ile Gln Pro Asn Gln Trp Phe Phe Val Ser Leu Pro
435 440 445
Met Pro Lys Lys Pro Val Lys Asn Thr Lys Gln Pro Lys Pro Lys His
450 455 460
Gln Ser Gln Ser Lys Pro Ala Asn Gly Ile Arg Gly Leu Ala Glu Leu
465 470 475 480
Arg Ala Gln Phe Gln Pro Ser Ile Thr His Ser Val Ser Ser Asp Asn
485 490 495
Gln Leu Ile Gln His Gln Pro Val Ile Thr Ser Leu Lys Ser Ile Asp
500 505 510
Tyr Gln Arg Ile Thr Arg Gln Leu Thr Ile Val Asn Arg Ile Arg Ser
515 520 525
Tyr His Gln Pro Lys His Ala Glu Tyr His Arg Gln Glu Leu Ile Asp
530 535 540
Ala Ile Ser Leu Phe Trp Gly Ser Asp Glu Leu Cys Ile Thr Asp Gly
545 550 555 560
Ile Phe Asp Glu Glu Ile Lys Val Leu Val Asn Gly Ser Thr Lys Pro
565 570 575
Val Ser Arg Lys Leu Lys Asn Ile Leu Arg Asn Asn Val Ile Lys Thr
580 585 590
Lys Phe Phe Asn Tyr Leu Val Arg Tyr Ile Glu Pro Lys Lys Val Ala
595 600 605
Asn Leu Leu His Asn Asn Tyr Gly Leu Ala Leu Phe Leu Val Gln Gln
610 615 620
Leu Thr Pro Ser Gln Leu Glu Ser Leu Met Ile Glu Tyr Ile Pro Leu
625 630 635 640
Glu Asp Glu Asn Gln Leu Val Gly Gln Thr Pro Arg Glu Glu Leu Thr
645 650 655
Glu Ala Gln Lys Gln Arg Leu Glu Gln Tyr Glu His Gln Leu Ala Met
660 665 670
Ile Phe Ser Glu Lys Ile Ser Leu Thr Tyr Cys Val Asn Arg Lys Met
675 680 685
Thr Val Gly Arg Leu Ala Leu Leu Asn His Ile
690 695
<210> 24
<211> 687
<212> PRT
<213> 宏基因组(metagenomic)
<400> 24
Met Glu Ser Glu Glu Phe Phe Lys Tyr Leu Lys Glu Gly Lys Tyr Glu
1 5 10 15
Lys Leu Ala Lys Leu Ile Asn Lys Asp Asp Thr Ala Leu Thr Phe Leu
20 25 30
Asp Glu Ala Leu Ser Ser Glu Val Lys Asp Leu Val Arg Arg Ser Phe
35 40 45
Leu Ile Leu Lys Arg Leu Asp Asp Glu Tyr Ile Val Arg Tyr Leu Pro
50 55 60
Tyr Ile Ile Leu Tyr Leu Lys Glu Lys Arg Ala Ile Ser Lys Glu Ala
65 70 75 80
Glu Glu Ile Leu Lys Asn Phe Thr Asn Ser Glu Asn Ile Asp Asp Ala
85 90 95
Ile Ile Asn Leu Leu Lys Met Pro Ile Asn Asp Ser Ile Leu Glu Glu
100 105 110
Leu Ile Tyr Leu Tyr Leu Glu Asn Ala Asn Ser Glu Asn Val Phe Tyr
115 120 125
Lys Ala Ile Lys Glu Tyr Ser Ala Ile Glu Glu Leu Met Lys Lys Tyr
130 135 140
Asp Ser Lys Arg Ile Leu Glu Ile Leu Thr His Lys Leu Tyr Ser Asp
145 150 155 160
Glu Lys Leu Glu Arg Asp Phe Ala Leu Asn Val Ile Tyr Asp Ile Val
165 170 175
Glu Leu Leu Ser Glu Asp Glu Lys Ala Ile Leu Lys Lys His Leu Asp
180 185 190
Ile Ser Ile Leu Gly Tyr Phe Asn Lys Arg Ser Tyr Lys Lys Ile Lys
195 200 205
His Leu Phe Glu Ile Leu Ser Ile Lys Pro Glu Ile Ser Glu Glu Asp
210 215 220
Ile Lys Lys Ile Leu Lys Thr Asp Gly Lys Ala Gly Leu Asn Ile Ile
225 230 235 240
Leu Arg Glu Asn Ile Ser Leu Ser Ser Lys Phe Tyr Asp Arg Glu Phe
245 250 255
Leu Arg Glu Tyr Leu Tyr Ser Gly Asn Glu Glu Lys Gln Phe Ile Gly
260 265 270
Val Lys Leu Ile Ser Leu Arg Ser Asp Ser Lys Ser Lys Ile Asp Leu
275 280 285
Leu Phe Lys Phe Leu Asn Tyr Gly Tyr Gly Lys Ala Lys Thr Ser Ala
290 295 300
Ile Arg Glu Leu Lys Lys Ile Ala Lys Asn Asn Ala Glu Leu Lys Ser
305 310 315 320
Tyr Ile Glu Asn Lys Thr Leu Met Tyr Ala Lys Lys Met Asn Leu Ser
325 330 335
Leu Lys Ile Ser Ser Leu Arg Ile Leu Lys Glu Ile Ala Lys Lys Glu
340 345 350
His Leu Asn Phe Leu Ile Asn Glu His Lys Arg Leu Lys Asn Leu Ala
355 360 365
Tyr Arg Leu Glu Glu Glu Lys Phe Met Gly Gly Phe Arg His Met Ile
370 375 380
Met Met Glu Glu Glu Ile Arg Lys Cys Asn Val Ala Met Asn Leu Ile
385 390 395 400
Glu Glu Thr Val Ala Arg Ile Cys Leu Asp Asn Asn Ile His Tyr Lys
405 410 415
Thr Leu Lys Ile Ala Glu Asn Leu Gly Tyr Glu Phe Tyr Lys Thr Ile
420 425 430
Glu Ile Ile Gly Ser Lys Asn Leu Asp Leu Ile Asn Ile Asn Glu Leu
435 440 445
Leu Asp Asp Ile Lys Arg Asp Gly Glu Leu Ile Ile Tyr Leu Thr Asn
450 455 460
Ile Ile Ala Asn Asn Arg Asn Ile Asp Glu His Leu Lys Asn Glu Ile
465 470 475 480
Ile Lys Val Ile Glu Asn Ile Asp Met Ala Asp Arg Asp Ile Leu Asn
485 490 495
Ala Asn Lys Ile Ile Ile Tyr Ser Ser Phe Asn Met Val Glu Lys Ile
500 505 510
Gly Glu Ile Ile Asn Met Ala Glu Gly Tyr Tyr Ser Lys Leu Ala Phe
515 520 525
Ile Asn Ala Val Lys Lys Phe Ile Glu Ser Gly Gln Phe Lys Glu Asp
530 535 540
His Leu Cys Tyr Val Val Pro Lys Ile Ala Glu Met Leu Tyr Ser Thr
545 550 555 560
Lys Lys Leu Arg Leu Met Ala Leu Glu Phe Phe Lys Glu Tyr Pro Asn
565 570 575
Glu Ile Ala Leu Pro Ile Leu Leu Lys Glu Leu Gly Asn His Arg Arg
580 585 590
Glu Glu Lys Leu Thr Ile Glu Val Ile Ser Asn Ile Ile Glu Lys Phe
595 600 605
Pro Tyr Ser Val Ile Lys Ile Lys Asn Leu Leu Asn Thr Glu Lys Arg
610 615 620
Glu Leu Ala Leu Lys Ile Leu Phe Lys Ile Ser Lys Asn Asn Pro Glu
625 630 635 640
Ile Leu Lys Asp Phe Thr Tyr Leu Leu Ala Gly Ile Tyr Glu Asn Ala
645 650 655
Ser Arg Glu Glu Lys Ser Leu Ile Gly Lys Ile Leu Arg Asn Ile Ile
660 665 670
Asn Glu Glu Gln Lys Leu Ile Leu Lys Pro Ile Ile Gly Asp Ile
675 680 685
<210> 25
<211> 768
<212> PRT
<213> 宏基因组(metagenomic)
<400> 25
Leu Asn Glu Ile Arg Gln Trp Thr Ala His Tyr Glu Ser Ala Tyr Lys
1 5 10 15
Ile Phe Ser Gly Ser Glu Leu Ile Lys Glu Glu Leu Gly Ala Asn Lys
20 25 30
Glu Pro Gln Asn Asn Arg Trp Met Val Ile Glu Ser Tyr Tyr Lys Gly
35 40 45
Leu Val Asn Arg Ile Asn Arg Gly Phe Leu Asn Asn Ser Ala Leu Tyr
50 55 60
Asn Phe Pro Leu Leu Phe Asp Leu Val Lys Pro Lys Asp Glu Asn Glu
65 70 75 80
Lys Lys Lys Leu Ile Glu Glu Tyr Tyr Lys Phe Ser Val Leu Lys Asp
85 90 95
Gly Lys Asn Val Gly Val Asn Leu Arg Thr Val Arg Glu Ala Met Phe
100 105 110
Lys Asn Gly Phe His Asp Val Lys Glu Lys Glu His Asp Thr Tyr Arg
115 120 125
Ser Lys Phe Tyr Gly Ile Val Asp Phe Leu Leu Tyr Arg Ser Glu Ser
130 135 140
Lys Asn Phe Ser Gly Trp Ser Glu Glu Leu Arg Lys Thr Val Asp Lys
145 150 155 160
Val Ala Lys Glu Lys Cys Tyr Asp Lys Trp Gly Glu Ser Val Ala Ala
165 170 175
Arg Met Arg Pro Lys Val Thr Ala Leu Leu Asp Lys Leu Ser Lys Met
180 185 190
Glu Trp Lys Lys Lys Pro Asn Lys Glu Asp Leu Glu Lys Leu Ala Glu
195 200 205
Pro Tyr Lys Ser Tyr Leu Ala Asp Val Gln Leu Ser Ala Asp Asp Ala
210 215 220
Asp Pro Leu Val Lys Leu Leu Ala Phe Leu Cys Asn Phe Trp Glu Gly
225 230 235 240
Lys Glu Ile Asn Glu Leu Leu Ser Ala Tyr Ile His Lys Phe Glu Asn
245 250 255
Ile Gln Glu Phe Ile Asp Thr Ile Glu Lys Leu Glu Gly Lys Lys Pro
260 265 270
Thr Phe Ser Glu Lys Tyr Ala Leu Phe Asn Glu Pro Ala Arg Arg Arg
275 280 285
Thr Glu Glu Val Ala Glu Ile Pro Gly Thr Arg Ala Gly Glu Ile Ala
290 295 300
Gln Asn Leu Arg Val Leu Ala Ser Ile Gly Lys Met Lys Pro Asp Leu
305 310 315 320
Gly Asp Ala Lys Arg Gln Leu Tyr Lys Ala Ala Ile Glu Met Leu Gly
325 330 335
Val Pro Asp Asp Ser Gln Phe Val Ser Asp Gln Trp Leu Ala Glu Asn
340 345 350
Val Leu Leu Asp Lys Thr Gln Ser Gly Tyr Ala Asn Arg Lys Thr Glu
355 360 365
Val Asn Pro Phe Arg Asn Phe Val Ala Lys Gln Val Ile Glu Ser Arg
370 375 380
Arg Phe Met Tyr Leu Val Arg Tyr Thr Lys Pro Lys Thr Val Arg Thr
385 390 395 400
Leu Met Glu Asn Lys Ala Val Val His Tyr Val Leu Ser Arg Ile Asp
405 410 415
Asn Ser Lys Asn Val Lys Gly Lys Glu Asp Ile Ile Thr Ser Tyr Tyr
420 425 430
Lys Thr Leu Lys Glu Tyr Asp Pro Gln Lys His Gln His Leu Pro Thr
435 440 445
Asp Lys Ala Glu Arg Glu Lys Glu Ile Asn Arg Arg Ile Asp Ala Leu
450 455 460
Ala Asp Tyr Leu Cys Lys Tyr Ser Phe Glu Lys Asn Val Leu Lys Gln
465 470 475 480
Lys Asp Gly Ile Val Arg Asn Thr Lys Ser Ala Thr Lys Asn Val Glu
485 490 495
Ile Glu Arg Leu Lys Ala Leu Thr Gly Leu Tyr Leu Thr Val Ala Tyr
500 505 510
Ile Ala Ile Lys Asn Leu Val Lys Ala Asn Ala Arg Tyr Tyr Ile Ala
515 520 525
Phe Ser Ile Phe Glu Arg Asp Tyr Ala Leu Phe Glu Lys Lys Leu Gly
530 535 540
Arg Asp Ala Leu Asp Glu Arg Pro Lys Tyr Pro Asp Asp Lys Gly Lys
545 550 555 560
Glu Thr Glu Ala Asp Tyr Asn Phe Phe Ala Leu Thr Lys Tyr Leu Leu
565 570 575
Asp Lys Asp Asp Lys Ala Phe Gly Ser Trp Lys Pro Tyr Gln Trp Asp
580 585 590
Glu Ser Lys Asn Lys Gly Glu Asn Trp Lys Ala Leu Arg Leu His Ile
595 600 605
Lys Gln His Lys Lys Glu Asn Gln Arg His Phe Ser Gln Lys Trp Leu
610 615 620
Asn Phe Phe Arg Gln Gln Ile Glu Asn Ala Lys Asn Ile Ser Glu Thr
625 630 635 640
Gly Tyr Leu Leu Thr Ala Ala Arg Asn His Ala Glu His Leu Asn Val
645 650 655
Leu Thr Ala Leu Pro Lys Phe Val Gly Glu Phe Arg Lys Thr Glu Gly
660 665 670
Lys Met Thr Ser Tyr Phe Glu Leu Tyr His Phe Leu Leu Gln Lys Leu
675 680 685
Met Leu Ala Asp Ala Gly Leu Lys Asn Leu Asp Lys Tyr Arg Glu Arg
690 695 700
Ile Asn Lys Tyr Gln Thr Ser Cys Lys Asp Leu Ile Lys Ile Thr Tyr
705 710 715 720
Val Ser Leu Gly Tyr Asn Leu Ala Arg Tyr Lys Asn Leu Thr Ile Glu
725 730 735
Ala Leu Phe Asp Ser Asp Ser Glu Asn Gly Lys Ala Leu Thr Lys Glu
740 745 750
Arg Glu Lys Lys Ala Glu Glu Arg Ala Lys Glu Arg Ala Lys Lys His
755 760 765
<210> 26
<211> 340
<212> RNA
<213> 宏基因组(metagenomic)
<400> 26
aauccaugac caaagaaucg ucacaaaucg aagcuuacaa aauggaguaa aauuuuuuuu 60
acucaguaau augcuuuggg uugaaagucu cccaccaauu cguaugcgga aaacguaaug 120
agauuuaaaa auuuuaaauu guuuaaauca acucauuaag gaggaugccc uuggguauuc 180
ugcuucuuga ccugguaccu cuauugcagg guacuggugu uuucuucggu acuggauucc 240
guuuguaugg aaucuaaacc auaguuauga cgauugcucu uucccgugcu ggaucgagua 300
acccaaugga gcuuacuauu cuugguccau ggauucaccc 340
<210> 27
<211> 699
<212> PRT
<213> 宏基因组(metagenomic)
<400> 27
Ala Ala Val Glu Asp Asn His Leu Leu Ile Lys Ala Val Gln Asn Glu
1 5 10 15
Asp Val Asp Leu Val Gln Gln Leu Leu Glu Gly Gly Ala Asn Val Asn
20 25 30
Phe Gln Glu Glu Glu Gly Gly Trp Thr Pro Leu His Asn Ala Val Gln
35 40 45
Met Ser Arg Glu Asp Ile Val Glu Leu Leu Leu Arg His Gly Ala Asp
50 55 60
Pro Val Leu Arg Lys Lys Asn Gly Ala Thr Pro Phe Ile Leu Ala Ala
65 70 75 80
Ile Ala Gly Ser Val Lys Leu Leu Lys Leu Phe Leu Ser Lys Gly Ala
85 90 95
Asp Val Asn Glu Cys Asp Phe Tyr Gly Phe Thr Ala Phe Met Glu Ala
100 105 110
Ala Val Tyr Gly Lys Val Lys Ala Leu Lys Phe Leu Tyr Lys Arg Gly
115 120 125
Ala Asn Val Asn Leu Arg Arg Lys Thr Lys Glu Asp Gln Glu Arg Leu
130 135 140
Arg Lys Gly Gly Ala Thr Ala Leu Met Asp Ala Ala Glu Lys Gly His
145 150 155 160
Val Glu Val Leu Lys Ile Leu Leu Asp Glu Met Gly Ala Asp Val Asn
165 170 175
Ala Cys Asp Asn Met Gly Arg Asn Ala Leu Ile His Ala Leu Leu Ser
180 185 190
Ser Asp Asp Ser Asp Val Glu Ala Ile Thr His Leu Leu Leu Asp His
195 200 205
Gly Ala Asp Val Asn Val Arg Gly Glu Arg Gly Lys Thr Pro Leu Ile
210 215 220
Leu Ala Val Glu Lys Lys His Leu Gly Leu Val Gln Arg Leu Leu Glu
225 230 235 240
Gln Glu His Ile Glu Ile Asn Asp Thr Asp Ser Asp Gly Lys Thr Ala
245 250 255
Leu Leu Leu Ala Val Glu Leu Lys Leu Lys Lys Ile Ala Glu Leu Leu
260 265 270
Cys Lys Arg Gly Ala Ser Thr Asp Cys Gly Asp Leu Val Met Thr Ala
275 280 285
Arg Arg Asn Tyr Asp His Ser Leu Val Lys Val Leu Leu Ser His Gly
290 295 300
Ala Lys Glu Asp Phe His Pro Pro Ala Glu Asp Trp Lys Pro Gln Ser
305 310 315 320
Ser His Trp Gly Ala Ala Leu Lys Asp Leu His Arg Ile Tyr Arg Pro
325 330 335
Met Ile Gly Lys Leu Lys Phe Phe Ile Asp Glu Lys Tyr Lys Ile Ala
340 345 350
Asp Thr Ser Glu Gly Gly Ile Tyr Leu Gly Phe Tyr Glu Lys Gln Glu
355 360 365
Val Ala Val Lys Thr Phe Cys Glu Gly Ser Pro Arg Ala Gln Arg Glu
370 375 380
Val Ser Cys Leu Gln Ser Ser Arg Glu Asn Ser His Leu Val Thr Phe
385 390 395 400
Tyr Gly Ser Glu Ser His Arg Gly His Leu Phe Val Cys Val Thr Leu
405 410 415
Cys Glu Gln Thr Leu Glu Ala Cys Leu Asp Val His Arg Gly Glu Asp
420 425 430
Val Glu Asn Glu Glu Asp Glu Phe Ala Arg Asn Val Leu Ser Ser Ile
435 440 445
Phe Lys Ala Val Gln Glu Leu His Leu Ser Cys Gly Tyr Thr His Gln
450 455 460
Asp Leu Gln Pro Gln Asn Ile Leu Ile Asp Ser Lys Lys Ala Ala His
465 470 475 480
Leu Ala Asp Phe Asp Lys Ser Ile Lys Trp Ala Gly Asp Pro Gln Glu
485 490 495
Val Lys Arg Asp Leu Glu Asp Leu Gly Arg Leu Val Leu Tyr Val Val
500 505 510
Lys Lys Gly Ser Ile Ser Phe Glu Asp Leu Lys Ala Gln Ser Asn Glu
515 520 525
Glu Val Val Gln Leu Ser Pro Asp Glu Glu Thr Lys Asp Leu Ile His
530 535 540
Arg Leu Phe His Pro Gly Glu His Val Arg Asp Cys Leu Ser Asp Leu
545 550 555 560
Leu Gly His Pro Phe Phe Trp Thr Trp Glu Ser Arg Tyr Arg Thr Leu
565 570 575
Arg Asn Val Gly Asn Glu Ser Asp Ile Lys Thr Arg Lys Ser Glu Ser
580 585 590
Glu Ile Leu Arg Leu Leu Gln Pro Gly Pro Ser Glu His Ser Lys Ser
595 600 605
Phe Asp Lys Trp Thr Thr Lys Ile Asn Glu Cys Val Met Lys Lys Met
610 615 620
Asn Lys Phe Tyr Glu Lys Arg Gly Asn Phe Tyr Gln Asn Thr Val Gly
625 630 635 640
Asp Leu Leu Lys Phe Ile Arg Asn Leu Gly Glu Asn Ile Asp Glu Glu
645 650 655
Lys His Lys Lys Met Lys Leu Lys Ile Gly Asp Pro Ser Leu Tyr Phe
660 665 670
Gln Lys Thr Phe Pro Asp Leu Val Ile Tyr Val Tyr Thr Lys Leu Gln
675 680 685
Asn Thr Glu Tyr Arg Lys His Phe Pro Gln Thr
690 695
<210> 28
<211> 104
<212> PRT
<213> 宏基因组(metagenomic)
<400> 28
Ala Cys Asp Tyr Thr Cys Gly Ser Asn Cys Tyr Ser Ser Ser Asp Val
1 5 10 15
Ser Thr Ala Gln Ala Ala Gly Tyr Lys Leu His Glu Asp Gly Glu Thr
20 25 30
Val Gly Ser Asn Ser Tyr Pro His Glu Phe Arg Asn Trp Asn Gly Phe
35 40 45
Asp Phe Ser Val Ser Ser Pro Tyr Tyr Glu Tyr Pro Ile Leu Ser Ser
50 55 60
Gly Asp Val Tyr Ser Gly Gly Ser Pro Gly Ala Asp Arg Val Val Phe
65 70 75 80
Asn Glu Asn Asn Gln Leu Ala Gly Val Ile Thr His Thr Gly Ala Ser
85 90 95
Gly Asn Asn Phe Val Glu Cys Thr
100
<210> 29
<211> 501
<212> PRT
<213> 宏基因组(metagenomic)
<400> 29
Gly Ala Gly His Met Ala Ser Pro Ser Glu Asn Leu Ser Arg Lys Ala
1 5 10 15
Lys Lys Lys Ala Ile Gln Gln Ser Pro Glu Ala Leu Leu Lys Gln Lys
20 25 30
Leu Asp Met Cys Ser Lys Lys Gly Asp Val Leu Glu Ala Leu Arg Leu
35 40 45
Tyr Asp Glu Ala Arg Arg Asn Gly Val Gln Leu Ser Gln Tyr His Tyr
50 55 60
Asn Val Leu Leu Tyr Val Cys Ser Leu Ala Glu Ala Ala Thr Glu Ser
65 70 75 80
Ser Pro Asn Pro Gly Leu Ser Arg Gly Phe Asp Ile Phe Lys Gln Met
85 90 95
Ile Val Asp Lys Val Val Pro Asn Glu Ala Thr Phe Thr Asn Gly Ala
100 105 110
Arg Leu Ala Val Ala Lys Asp Asp Pro Glu Met Ala Phe Asp Met Val
115 120 125
Lys Gln Met Lys Ala Phe Gly Ile Gln Pro Arg Leu Arg Ser Tyr Gly
130 135 140
Pro Ala Leu Phe Gly Phe Cys Arg Lys Gly Asp Ala Asp Lys Ala Tyr
145 150 155 160
Glu Val Asp Ala His Met Val Glu Ser Glu Val Val Pro Glu Glu Pro
165 170 175
Glu Leu Ala Ala Leu Leu Lys Val Ser Met Asp Thr Lys Asn Ala Asp
180 185 190
Lys Val Tyr Lys Thr Leu Gln Arg Leu Arg Asp Leu Val Arg Gln Val
195 200 205
Ser Lys Ser Thr Phe Asp Met Ile Glu Glu Trp Phe Lys Ser Glu Val
210 215 220
Ala Thr Lys Thr Gly Val Lys Lys Trp Asp Val Lys Lys Ile Arg Asp
225 230 235 240
Ala Val Val Ser Gly Gly Gly Gly Trp His Gly Gln Gly Trp Leu Gly
245 250 255
Thr Gly Lys Trp Asn Val Lys Arg Thr Glu Met Asp Glu Asn Gly Val
260 265 270
Cys Lys Cys Cys Lys Glu Lys Leu Val Cys Ile Asp Ile Asn Pro Val
275 280 285
Glu Thr Glu Thr Phe Ala Ala Ser Leu Thr Arg Leu Ala Cys Glu Arg
290 295 300
Glu Val Lys Ala Asn Phe Asn Gln Phe Gln Glu Trp Leu Glu Arg His
305 310 315 320
Gly Pro Phe Asp Ala Val Ile Asp Gly Ala Asn Met Gly Leu Val Asn
325 330 335
Gln Arg Ser Phe Ser Phe Phe Gln Leu Asn Asn Thr Val Gln Arg Cys
340 345 350
Gln Gln Ile Ser Pro Ser Lys Arg Leu Pro Leu Val Ile Leu His Lys
355 360 365
Ser Arg Val Asn Gly Gly Pro Ala Thr Tyr Pro Lys Asn Arg Ala Leu
370 375 380
Leu Glu Lys Trp Lys Asn Ala Gly Ala Leu Tyr Ala Thr Pro Pro Gly
385 390 395 400
Ser Asn Asp Asp Trp Tyr Trp Leu Tyr Ala Ala Val Ser Cys Lys Cys
405 410 415
Leu Leu Val Thr Asn Asp Glu Met Arg Asp His Leu Phe Gln Leu Leu
420 425 430
Gly Asn Ser Phe Phe Pro Arg Trp Lys Glu Lys His Gln Val Arg Ile
435 440 445
Ser Val Thr Arg Glu Asp Gly Leu Lys Leu Asn Met Pro Pro Pro Tyr
450 455 460
Ser Ile Val Ile Gln Glu Ser Glu Asp Gly Thr Trp His Val Pro Met
465 470 475 480
Ser Val Glu Asp Asp Leu Gln Thr Ser Arg Gln Trp Leu Cys Ala Lys
485 490 495
Arg Ser Lys Thr Pro
500
<210> 30
<211> 96
<212> PRT
<213> 宏基因组(metagenomic)
<400> 30
Gly Ala His Arg Gly Arg Val Thr Arg Arg Asn Ile Ile Trp His Glu
1 5 10 15
Leu Ile Gly Leu Arg Val Arg Ile Val Gly Ser Thr His Pro Ala Phe
20 25 30
Val Gly Ile Glu Gly Tyr Val Ile Asp Glu Thr Arg Asn Met Leu Val
35 40 45
Ile Ala Gly Asp Arg Ile Trp Lys Val Pro Lys Asp Val Ser Ile Phe
50 55 60
Glu Phe Glu Ala Asp Asp Gly Thr Lys Ile Lys Ile Pro Gly Glu Arg
65 70 75 80
Leu Val Gly Arg Pro Glu Met Arg Leu Lys Lys Arg Trp Lys Lys Trp
85 90 95
<210> 31
<211> 165
<212> PRT
<213> 宏基因组(metagenomic)
<400> 31
Gly Ala Met Gly Gln Tyr Glu Gly Val Phe Tyr Thr Asp Gly Ser Ala
1 5 10 15
Ile Lys Ser Pro Asp Pro Thr Lys Ser Asn Asn Ala Gly Met Gly Ile
20 25 30
Val His Ala Thr Tyr Lys Pro Glu Tyr Gln Val Leu Asn Gln Trp Ser
35 40 45
Ile Pro Leu Gly Asn His Thr Ala Gln Met Ala Glu Ile Ala Ala Val
50 55 60
Glu Phe Ala Cys Lys Lys Ala Leu Lys Ile Pro Gly Pro Val Leu Val
65 70 75 80
Ile Thr Asp Ser Phe Tyr Val Ala Glu Ser Ala Asn Lys Glu Leu Pro
85 90 95
Tyr Trp Lys Ser Asn Gly Phe Val Asn Asn Lys Lys Lys Pro Leu Lys
100 105 110
His Ile Ser Lys Trp Lys Ser Ile Ala Glu Cys Leu Ser Met Lys Pro
115 120 125
Asp Ile Thr Ile Gln His Glu Lys Gly His Gln Pro Thr Asn Thr Ser
130 135 140
Ile His Thr Glu Gly Asn Ala Leu Ala Asp Lys Leu Ala Thr Gln Gly
145 150 155 160
Ser Tyr Val Val Asn
165
<210> 32
<211> 27
<212> RNA
<213> 宏基因组(metagenomic)
<400> 32
ggaagaccgg ucuucggacc ggcuucc 27
<210> 33
<211> 27
<212> RNA
<213> 宏基因组(metagenomic)
<400> 33
ggaagcucgg ucuucggacc ggcuucc 27
<210> 34
<211> 46
<212> RNA
<213> 宏基因组(metagenomic)
<400> 34
ggacucagua auaugcuuug gaaacgaagc uuacaaaaug gagucc 46
<210> 35
<211> 347
<212> RNA
<213> 宏基因组(metagenomic)
<400> 35
ggagaggagc aggcggucgc gggggcgcac accugcgcuu cccgaggaaa guccggacuc 60
uggagcgggg ugccggguaa cgcccgggag gggugacccu cggacagggc cauagagaag 120
aagaccgccc ggggggaaac uuccgggcaa ggguggaacg gugggguaag agcccaccag 180
cgucggggca acccggcggc uuggcaaccc ccaccuggag caaggccaag caggggguug 240
ggucgcuccc ccuauucccc cggguuggcc gcuugaggug ugcgguaacg cacaccccag 300
auugaugacc gcccacgaca gaauccggcu uaugcuccuc ucccgug 347
<210> 36
<211> 338
<212> RNA
<213> 宏基因组(metagenomic)
<400> 36
ggagaggagc aggcggucgc gggggcgcac accugcgcuu cccgaggaaa guccggacuc 60
uggagcgggg ugccggguaa cgcccgggag gggugacccu cggacagggc cauagagaag 120
aagaccgccc guugagggca aggguggaac ggugggguaa gagcccacca gcgucggggc 180
aacccggcgg cuuggcaacc cccaccugga gcaaggccaa gcaggggguu gggucgcucc 240
cccuauuccc ccggguuggc cgcuugaggu gugcgguaac gcacacccca gauugaugac 300
cgcccacgac agaauccggc uuaugcuccu cucccgug 338
<210> 37
<211> 284
<212> PRT
<213> 宏基因组(metagenomic)
<400> 37
Met Val Leu Met Ile Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val
1 5 10 15
Ala Leu Arg Ala Leu Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu
20 25 30
Pro Val Val Leu Leu Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu
35 40 45
Ile Ser Ala Ala Val Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro
50 55 60
Glu Ile Phe Glu Gln Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro
65 70 75 80
Gln Leu Leu Phe Leu Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr
85 90 95
Ser Asp Thr Arg Arg Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu
100 105 110
Glu Ser Ala Ile Asp Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser
115 120 125
Arg Ala Asp Leu Ile Val Asp Thr Ser Glu Met Ser Val His Glu Leu
130 135 140
Ala Glu Met Leu Arg Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu
145 150 155 160
Thr Met Val Phe Glu Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp
165 170 175
Ala Asp Tyr Val Phe Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp
180 185 190
Pro Lys Leu Arg Pro Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe
195 200 205
Leu Asp Arg His Thr Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser
210 215 220
Tyr Leu Glu Leu Trp Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr
225 230 235 240
Leu Thr Val Ala Ile Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr
245 250 255
Ile Ala Glu Gln Leu Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val
260 265 270
Gln Ser Arg His Arg Thr Leu Glu Lys Arg Lys Pro
275 280
<210> 38
<211> 165
<212> PRT
<213> 宏基因组(metagenomic)
<400> 38
Gly Ser Ile Leu Ala Glu Thr His Gly Thr Arg Pro Asp Leu Thr Asp
1 5 10 15
Gln Pro Ile Pro Asp Ala Asp Tyr Thr Trp Tyr Thr Asp Gly Ser Ser
20 25 30
Phe Leu Gln Glu Gly Gln Arg Arg Ala Gly Ala Ala Val Thr Thr Glu
35 40 45
Thr Glu Val Ile Trp Ala Arg Ala Leu Pro Ala Gly Thr Ser Ala Gln
50 55 60
Arg Ala Glu Leu Ile Ala Leu Thr Gln Ala Leu Lys Met Ala Glu Gly
65 70 75 80
Lys Lys Leu Asn Val Tyr Thr Asp Ser Arg Tyr Ala Phe Ala Thr Ala
85 90 95
His Val His Ser Glu Gly Arg Glu Ile Lys Asn Lys Asn Glu Ile Leu
100 105 110
Ala Leu Leu Lys Ala Leu Phe Leu Pro Lys Arg Leu Ser Ile Ile His
115 120 125
Cys Pro Gly His Gln Lys Gly Asn Ser Ala Glu Ala Arg Gly Asn Arg
130 135 140
Met Ala Asp Gln Ala Ala Arg Glu Ala Ala Met Lys Ala Val Leu Glu
145 150 155 160
Thr Ser Thr Leu Leu
165
<210> 39
<211> 123
<212> PRT
<213> 宏基因组(metagenomic)
<400> 39
Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala
1 5 10 15
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr His His Pro
20 25 30
Tyr Ile Tyr Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu
35 40 45
Gly Val Ala Ala Met Asp Ser Gly Gly Gly Gly Thr Leu Tyr Ala Asp
50 55 60
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Gly Lys Asn Thr
65 70 75 80
Val Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Thr Tyr
85 90 95
Tyr Cys Ala Ala Gly Gly His His Leu Arg Asp His Thr Tyr Gly Gln
100 105 110
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
115 120
<210> 40
<211> 268
<212> RNA
<213> 宏基因组(metagenomic)
<400> 40
guuaaucaug cucggguaau cgcugcggcc gguuucggcc guagaggaaa guccaugcuc 60
gcacggugcu gagaugcccg uaguguucgu ggaaacacga gcgagaaacc caaaugaugg 120
uaggggcacc uucccgaagg aaaugaacgg agggaaggac aggcggcgca ugcagccugu 180
agauagauga uuaccgccgg aguacgaggc gcaaagccgc uugcaguacg aagguacaga 240
acauggcuua uagagcauga uuaacguc 268
<210> 41
<211> 231
<212> PRT
<213> 宏基因组(metagenomic)
<400> 41
Lys His Pro Pro Leu Pro Phe Ile Lys Asp Gln Thr Leu Tyr Glu Arg
1 5 10 15
Val Phe Val His Lys Ser Val Val Asn Gly Lys Thr Tyr Leu Asp Gln
20 25 30
Asn Asp Leu Ile Asn Ser His Asn Glu Arg Leu Glu Phe Leu Gly Asp
35 40 45
Ser Val Leu Asn Asn Leu Val Thr Leu Ile Ile Tyr Asp Lys Phe Pro
50 55 60
Ser Ala Ser Glu Gly Lys Leu Thr Lys Met Arg Ser Gln Leu Ile Asp
65 70 75 80
Asn His Thr Leu Thr Gln Phe Ser Phe Glu Tyr Gly Phe Asp Lys Arg
85 90 95
Leu Lys Thr Lys Thr Asp Glu Glu Ile Leu Lys Thr Gly Asp Gln Lys
100 105 110
Val Tyr Ala Asp Ile Phe Glu Ala Tyr Ile Gly Ala Leu Ser Val Glu
115 120 125
Arg Gly Leu Asp Leu Arg Glu Ile Lys Asp Trp Leu Glu Lys Leu Tyr
130 135 140
Ala Pro Lys Leu Glu Ala Phe Lys Val Asn Phe Leu Gln Glu Ser Val
145 150 155 160
Asn Lys Glu Ala Lys Ser Glu Leu Tyr Ser Ile Val Gly Thr Ala Ser
165 170 175
Ser His Pro Leu Tyr Val Val Val Glu Glu Gly Asn Gly Ser His Asp
180 185 190
Phe Val Val Glu Cys Arg Met Gly Asn Asp Val Leu Gly Arg Ala Lys
195 200 205
Ala Pro Ser Gln Lys Glu Ala Gly Leu Arg Ala Ala Met Asp Ala Leu
210 215 220
Lys Asn Arg Gln Leu Leu Glu
225 230
<210> 42
<211> 267
<212> PRT
<213> 宏基因组(metagenomic)
<400> 42
Met Ala Ala Leu Thr Pro Ser Arg Ser Val Phe Ala Ser Thr Cys Lys
1 5 10 15
Thr Ile Ala Gln Gln Cys Ser Arg Arg Pro Val Ala Val Gln Gln Leu
20 25 30
Ala Ala Val Pro Val Ala Arg Gln Val Ser Ser Ser Ser Ala Val Gln
35 40 45
Gln Glu Gln Asp Ala Ser Gly Thr Ser Ser Ser Gln Gln Pro Arg Pro
50 55 60
Arg Trp Ser Tyr Thr Pro Glu Arg Met Lys Gly Pro Gly Phe Ser Leu
65 70 75 80
Asn Leu Val Lys Asp Pro Arg Arg Lys Gln Trp Met Val Asn Ser Asp
85 90 95
Pro Ala Lys Leu Asp Ala Phe Tyr Asp Ala Phe Leu Gly Gln Gly Gly
100 105 110
Ser Arg Met Leu Ser Glu Glu Thr Lys Trp Leu Ala Val Thr His Lys
115 120 125
Ser Phe Asp Tyr Gly Arg Arg Gly Tyr Asn Thr Arg Leu Ala Phe Leu
130 135 140
Gly Arg Gln Ile Ile Ala Leu Glu Thr Thr Arg Ser Ile Leu Thr Ser
145 150 155 160
Pro Val Leu Asn Glu Pro Ile Val Asp Lys Tyr Gly Arg Thr Pro Tyr
165 170 175
Asn His Ala Ala Leu Ala Asn Ile Asp Lys Leu Ile Tyr Thr Gln Pro
180 185 190
Leu Asp Ile Met Asp Lys Thr Lys Ile Ala Arg Met Gly Ile Asp Phe
195 200 205
Gly Leu Leu Thr Val Met Arg Trp Lys Pro Arg Met Pro Glu Asp Leu
210 215 220
Glu Ser Ser Gly Val Val Val Val Leu Asn Ser Thr Leu Phe Ala Ile
225 230 235 240
Ile Gly Ala Ile Ser Leu Glu Lys Gly Ala Ala Val Ala Gln Arg Ile
245 250 255
Val Arg Glu Lys Ile Leu Lys Arg Leu Gly Ala
260 265
<210> 43
<211> 221
<212> PRT
<213> 宏基因组(metagenomic)
<400> 43
Met Ala Tyr Val Gly Gln Ser Arg Asn Val Ile Arg His Val Val Ser
1 5 10 15
Arg Gly Thr Ala Tyr His Lys Tyr Glu Asn Ala Ile His His Pro Leu
20 25 30
Leu Phe Ala Cys Gln Gly Val Arg Tyr Arg Lys Leu Glu Val Ile Leu
35 40 45
Thr Thr Gly Ile Glu Lys Leu Gly Lys Ala Gly Glu Thr Val Lys Val
50 55 60
Ala Pro Gly Tyr Phe Arg Asn His Leu Met Pro Lys Leu Leu Ala Val
65 70 75 80
Pro Asn Ile Asp Lys Tyr Ala Tyr Leu Ile Arg Glu Gln Arg Lys Met
85 90 95
Tyr Asn His Glu Glu Val Lys Glu Glu Val Lys Val Val His Lys Thr
100 105 110
Ser Glu Val Gln Thr Lys Glu Tyr Glu Lys Ala Ala Lys Arg Leu Ala
115 120 125
Asn Ala Asn Leu Val Leu Arg Lys Leu Val Asp Lys Glu Lys Phe Lys
130 135 140
Asn Arg Ser Ser Lys Asp Asp Lys Pro Asp Val Gln Thr Pro Ile Thr
145 150 155 160
Lys Glu Glu Ile Val Ser Glu Val Ala Arg Gln Leu Cys Val Lys Ile
165 170 175
Asp Pro Asp Asn Val Val Leu Ala Ala Pro Leu Glu Thr Phe Gly Glu
180 185 190
Tyr Glu Val Pro Leu Lys Phe Pro Lys Thr Ile Pro Leu Pro Gln Gly
195 200 205
Thr Val Gln Trp Ile Leu Lys Val Lys Val Arg Gly His
210 215 220
<210> 44
<211> 279
<212> PRT
<213> 宏基因组(metagenomic)
<400> 44
Met Asp Arg Thr Gln Thr Phe Ile Lys Asp Cys Leu Phe Thr Lys Cys
1 5 10 15
Leu Glu Asp Pro Glu Lys Pro Phe Asn Glu Asn Arg Phe Gln Asp Thr
20 25 30
Leu Leu Leu Leu Pro Thr Asp Gly Gly Leu Thr Ser Arg Leu Gln Arg
35 40 45
Gln Gln Arg Lys Ser Lys Leu Asn Leu Asp Asn Leu Gln Lys Val Ser
50 55 60
Gln Leu Glu Ser Ala Asp Lys Gln Leu Glu Lys Arg Asp Tyr Gln Arg
65 70 75 80
Ile Asn Lys Asn Ser Lys Ile Ala Leu Arg Glu Tyr Ile Asn Asn Cys
85 90 95
Lys Lys Asn Thr Lys Lys Cys Leu Lys Leu Ala Tyr Glu Asn Lys Ile
100 105 110
Thr Asp Lys Glu Asp Leu Leu His Tyr Ile Glu Glu Lys His Pro Thr
115 120 125
Ile Tyr Glu Ser Leu Pro Gln Tyr Val Asp Phe Val Pro Met Tyr Lys
130 135 140
Glu Leu Trp Ile Asn Tyr Ile Lys Glu Leu Leu Asn Ile Thr Lys Asn
145 150 155 160
Leu Lys Thr Phe Asn Gly Ser Leu Ala Leu Leu Lys Leu Ser Met Ala
165 170 175
Asp Tyr Asn Gly Ala Leu Leu Arg Val Thr Lys Ser Lys Asn Lys Thr
180 185 190
Leu Ile Gly Leu Gln Gly Ile Val Ile Trp Asp Ser Gln Lys Phe Phe
195 200 205
Ile Met Ile Val Lys Gly Asn Ile Ile Asp Glu Ile Lys Cys Ile Pro
210 215 220
Lys Lys Gly Thr Val Phe Gln Phe Glu Ile Pro Ile Ser Asp Asp Asp
225 230 235 240
Asp Ser Ala Leu Arg Tyr Ser Ile Leu Gly Asp Arg Phe Lys Tyr Arg
245 250 255
Ser Val Asp Arg Ala Gly Arg Lys Phe Lys Ser Arg Arg Cys Asp Asp
260 265 270
Met Leu Tyr Tyr Ile Gln Asn
275
<210> 45
<211> 538
<212> PRT
<213> 宏基因组(metagenomic)
<400> 45
Met Glu Gly Glu Val Ile His Arg Tyr Lys Val Asn Gly Phe Lys Leu
1 5 10 15
Phe Gly Leu Pro Thr Pro Lys Asn Asn Thr Ile Leu Gly Val Leu Gly
20 25 30
Lys Asn Gly Val Gly Lys Thr Thr Val Leu Lys Ile Leu Ala Gly Glu
35 40 45
Ile Ile Pro Asn Phe Gly Asp Pro Asn Ser Lys Val Gly Lys Asp Glu
50 55 60
Val Leu Lys Arg Phe Arg Gly Lys Glu Ile Tyr Asn Tyr Phe Lys Glu
65 70 75 80
Leu Tyr Ser Asn Glu Leu Lys Ile Val His Lys Ile Gln Tyr Val Glu
85 90 95
Tyr Ala Ser Lys Phe Leu Lys Gly Thr Val Asn Glu Ile Leu Thr Lys
100 105 110
Ile Asp Glu Arg Gly Lys Lys Asp Glu Val Lys Glu Leu Leu Asn Met
115 120 125
Thr Asn Leu Trp Asn Lys Asp Ala Asn Ile Leu Ser Gly Gly Gly Leu
130 135 140
Gln Arg Leu Leu Val Ala Ala Ser Leu Leu Arg Glu Ala Asp Val Tyr
145 150 155 160
Ile Phe Asp Gln Pro Ser Ser Tyr Leu Asp Val Arg Glu Arg Met Asn
165 170 175
Met Ala Lys Ala Ile Arg Glu Leu Leu Lys Asn Lys Tyr Val Ile Val
180 185 190
Val Asp His Asp Leu Ile Val Leu Asp Tyr Leu Thr Asp Leu Ile His
195 200 205
Ile Ile Tyr Gly Glu Ser Ser Val Tyr Gly Arg Val Ser Lys Ser Tyr
210 215 220
Ala Ala Arg Val Gly Ile Asn Asn Phe Leu Lys Gly Tyr Leu Pro Ala
225 230 235 240
Glu Asn Met Lys Ile Arg Pro Asp Glu Ile Lys Phe Met Leu Lys Glu
245 250 255
Val Ser Asp Leu Asp Leu Ser Lys Asp Leu Lys Thr Lys Met Lys Trp
260 265 270
Thr Lys Ile Ile Lys Lys Leu Gly Asp Phe Gln Leu Val Val Asp Asn
275 280 285
Gly Glu Ala Lys Glu Gly Glu Ile Ile Gly Ile Leu Gly Pro Asn Gly
290 295 300
Ile Gly Lys Thr Thr Phe Ala Arg Ile Leu Val Gly Glu Ile Thr Ala
305 310 315 320
Asp Glu Gly Ser Val Thr Pro Glu Lys Gln Ile Leu Ser Tyr Lys Pro
325 330 335
Gln Arg Ile Phe Pro Asn Tyr Asp Gly Thr Val Gln Gln Tyr Leu Glu
340 345 350
Asn Ala Ser Lys Asp Ala Leu Ser Thr Ser Ser Trp Phe Phe Glu Glu
355 360 365
Val Thr Lys Arg Leu Asn Leu His Arg Leu Leu Glu Ser Asn Val Asn
370 375 380
Asp Leu Ser Gly Gly Glu Leu Gln Lys Leu Tyr Ile Ala Ala Thr Leu
385 390 395 400
Ala Lys Glu Ala Asp Leu Tyr Val Leu Asp Gln Pro Ser Ser Tyr Leu
405 410 415
Asp Val Glu Glu Arg Tyr Ile Val Ala Lys Ala Ile Lys Arg Val Thr
420 425 430
Arg Glu Arg Lys Ala Val Thr Phe Ile Ile Asp His Asp Leu Ser Ile
435 440 445
His Asp Tyr Ile Ala Asp Arg Ile Ile Val Phe Lys Gly Glu Pro Glu
450 455 460
Lys Ala Gly Leu Ala Thr Ser Pro Val Thr Leu Lys Thr Gly Met Asn
465 470 475 480
Glu Phe Leu Arg Glu Leu Glu Val Thr Phe Arg Arg Asp Ala Glu Thr
485 490 495
Gly Arg Pro Arg Val Asn Lys Ile Gly Ser Tyr Leu Asp Arg Val Gln
500 505 510
Lys Glu Arg Gly Asp Tyr Tyr Ser Met Val Leu Ser Thr Gln Gly Ser
515 520 525
Ile Glu Gly Arg His His His His His His
530 535
<210> 46
<211> 165
<212> PRT
<213> 宏基因组(metagenomic)
<400> 46
Met Gly His His His His His His Gly Gly Met Ile Thr Tyr Gly Ser
1 5 10 15
Gly Asp Leu Leu Arg Ala Asp Thr Glu Ala Leu Val Asn Thr Val Asn
20 25 30
Cys Val Gly Val Met Gly Lys Gly Ile Ala Leu Gln Phe Lys Arg Arg
35 40 45
Tyr Pro Glu Met Phe Thr Ala Tyr Glu Lys Ala Cys Lys Arg Gly Glu
50 55 60
Val Thr Ile Gly Lys Met Phe Val Val Asp Thr Gly Gln Leu Asp Gly
65 70 75 80
Pro Lys His Ile Ile Asn Phe Pro Thr Lys Lys His Trp Arg Ala Pro
85 90 95
Ser Lys Leu Ala Tyr Ile Asp Ala Gly Leu Ile Asp Leu Ile Arg Val
100 105 110
Ile Arg Glu Leu Asn Ile Ala Ser Val Ala Val Pro Pro Leu Gly Val
115 120 125
Gly Asn Gly Gly Leu Asp Trp Glu Asp Val Glu Gln Arg Leu Val Ser
130 135 140
Ala Phe Gln Gln Leu Pro Asp Val Asp Ala Val Ile Tyr Pro Pro Ser
145 150 155 160
Gly Gly Ser Arg Ala
165
<210> 47
<211> 470
<212> PRT
<213> 宏基因组(metagenomic)
<400> 47
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro
1 5 10 15
Arg Gly Ser His Met Ala Ser Thr Glu Ile Gly Ile Ile Ala Val Gly
20 25 30
Gly Tyr Asn Glu Met Gly Arg Asn Met Thr Ala Ile Arg Val Asn Glu
35 40 45
Asp Ile Ile Ile Ile Asp Met Gly Ile Arg Leu Asp Arg Val Gln Ile
50 55 60
His Glu Asp Val Asp Thr Asp Arg Met His Ser Leu Glu Leu Ile Glu
65 70 75 80
Met Gly Ala Ile Pro Asp Asp Thr Ile Met Asn Glu Val Asn Gly Asn
85 90 95
Val Arg Ala Ile Val Cys Thr His Gly His Leu Asp His Ile Gly Ala
100 105 110
Ile Pro Lys Leu Ala His Arg Tyr Ala Ala Pro Ile Ile Ala Thr Pro
115 120 125
Tyr Thr Thr Ala Leu Ile Lys His Gln Ile Asp Ser Glu Arg Lys Phe
130 135 140
Gly Val Lys Asn Asn Ile Val Ala Leu Lys Ala Gly Glu Thr Leu Glu
145 150 155 160
Ile Thr Lys Asp Ile Thr Ile Glu Phe Ile Asn Thr Gln His Ser Ile
165 170 175
Ile Asp Thr Val Phe Val Ala Ile His Thr Pro Ser Gly Ala Val Val
180 185 190
Tyr Ala Cys Asp Phe Lys Phe Asp Arg Thr Pro Thr Leu Gly Glu Val
195 200 205
Pro Asp Phe Asp Arg Leu Lys Glu Leu Gly Lys Glu Gly Val Ile Ala
210 215 220
Leu Ile Thr Glu Ser Thr Asn Ala Gly Arg Asn Gly Lys Thr Pro Ser
225 230 235 240
Glu Leu Ile Ala His Met Met Leu Lys Asp Val Leu Leu Gly Thr Glu
245 250 255
Glu Ser Ala Val Gly Met Ile Val Thr Thr Phe Ala Ala His Ile Ala
260 265 270
Arg Val Asn Ser Ile Val Gln Phe Ala Gln Glu Met Gly Arg Ile Pro
275 280 285
Val Leu Leu Gly Arg Ser Met Glu Arg Tyr Val Gly Thr Ala Tyr Gln
290 295 300
Leu Gly Tyr Ile Asp Leu Pro Glu Asn Val Glu Ile Tyr Gly Ser Arg
305 310 315 320
Arg Asp Ile Asp Asn Ala Leu Lys Lys Ile Met Glu Ala Gly Lys Asp
325 330 335
Lys Tyr Leu Pro Val Met Thr Gly His Gln Gly Glu Pro Gly Ala Val
340 345 350
Leu Gly Arg Ile Ala Asn Gly Glu Thr Pro Phe Lys Val Glu Thr Gly
355 360 365
Asp Arg Ile Ile Phe Ser Ala Asn Val Ile Pro Asn Pro Met Thr Gln
370 375 380
Ala Asn Arg Tyr Ala Leu Glu Thr Lys Leu Lys Met Lys Gly Ala Arg
385 390 395 400
Ile Tyr Asp Asn Val His Val Ser Gly His Ala Tyr Arg Glu Asp His
405 410 415
Trp Glu Leu Leu Arg Met Leu Lys Pro Glu His Val Ile Pro Ala His
420 425 430
Gly Thr Ile Gln Met His Ser Glu Tyr Ile Gln Met Ala Glu Asp Ala
435 440 445
Gly Tyr Ser Leu Gly Asp Thr Leu His Leu Leu Arg Asn Gly Glu Glu
450 455 460
Leu Tyr Ile Glu Glu Asp
465 470
<210> 48
<211> 538
<212> PRT
<213> 宏基因组(metagenomic)
<400> 48
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro
1 5 10 15
Arg Ser His Met Glu Gln Leu Glu Glu Asp Cys Val His Arg Tyr Gly
20 25 30
Val Asn Ala Phe Val Leu Tyr Arg Leu Pro Val Val Lys Glu Gly Met
35 40 45
Val Val Gly Ile Val Gly Pro Asn Gly Thr Gly Lys Ser Thr Ala Val
50 55 60
Lys Ile Leu Ala Gly Gln Leu Ile Pro Asn Leu Cys Gly Asp Asn Asp
65 70 75 80
Ser Trp Asp Gly Val Ile Arg Ala Phe Arg Gly Asn Glu Leu Gln Asn
85 90 95
Tyr Phe Glu Lys Leu Lys Asn Gly Glu Ile Arg Pro Val Val Lys Pro
100 105 110
Gln Tyr Val Asp Leu Ile Pro Lys Ala Val Lys Gly Lys Val Ile Glu
115 120 125
Leu Leu Lys Lys Ala Asp Glu Thr Gly Lys Leu Glu Glu Val Val Lys
130 135 140
Ala Leu Glu Leu Glu Asn Val Leu Glu Arg Glu Ile Gln His Leu Ser
145 150 155 160
Gly Gly Glu Leu Gln Arg Val Ala Ile Ala Ala Ala Leu Leu Arg Asn
165 170 175
Ala Thr Phe Tyr Phe Phe Asp Glu Pro Ser Ser Tyr Leu Asp Ile Arg
180 185 190
Gln Arg Leu Asn Ala Ala Arg Ala Ile Arg Arg Leu Ser Glu Glu Gly
195 200 205
Lys Ser Val Leu Val Val Glu His Asp Leu Ala Val Leu Asp Tyr Leu
210 215 220
Ser Asp Ile Ile His Val Val Tyr Gly Glu Pro Gly Val Tyr Gly Ile
225 230 235 240
Phe Ser Gln Pro Lys Gly Thr Arg Asn Gly Ile Asn Glu Phe Leu Arg
245 250 255
Gly Tyr Leu Lys Asp Glu Asn Val Arg Phe Arg Pro Tyr Glu Ile Lys
260 265 270
Phe Thr Lys Thr Gly Glu Arg Val Glu Ile Glu Arg Glu Thr Leu Val
275 280 285
Thr Tyr Pro Arg Leu Val Lys Asp Tyr Gly Ser Phe Arg Leu Glu Val
290 295 300
Glu Pro Gly Glu Ile Lys Lys Gly Glu Val Ile Gly Ile Val Gly Pro
305 310 315 320
Asn Gly Ile Gly Lys Thr Thr Phe Val Lys Met Leu Ala Gly Val Glu
325 330 335
Glu Pro Thr Glu Gly Lys Ile Glu Trp Asp Leu Thr Val Ala Tyr Lys
340 345 350
Pro Gln Tyr Ile Lys Ala Asp Tyr Glu Gly Thr Val Tyr Glu Leu Leu
355 360 365
Ser Lys Ile Asp Ala Ser Lys Leu Asn Ser Asn Phe Tyr Lys Thr Glu
370 375 380
Leu Leu Lys Pro Leu Gly Ile Ile Asp Leu Tyr Asp Arg Glu Val Asn
385 390 395 400
Glu Leu Ser Gly Gly Glu Leu Gln Arg Val Ala Ile Ala Ala Thr Leu
405 410 415
Leu Arg Asp Ala Asp Ile Tyr Leu Leu Asp Glu Pro Ser Ala Tyr Leu
420 425 430
Asp Val Glu Gln Arg Leu Ala Val Ser Arg Ala Ile Arg His Leu Met
435 440 445
Glu Lys Asn Glu Lys Thr Ala Leu Val Val Glu His Asp Val Leu Met
450 455 460
Ile Asp Tyr Val Ser Asp Arg Leu Met Val Phe Glu Gly Glu Pro Gly
465 470 475 480
Lys Tyr Gly Arg Ala Leu Pro Pro Met Gly Met Arg Glu Gly Met Asn
485 490 495
Arg Phe Leu Ala Ser Ile Gly Ile Thr Phe Arg Arg Asp Pro Asp Thr
500 505 510
Gly Arg Pro Arg Ala Asn Lys Glu Gly Ser Val Lys Asp Arg Glu Gln
515 520 525
Lys Glu Lys Gly Glu Tyr Tyr Tyr Ile Ala
530 535
<210> 49
<211> 572
<212> PRT
<213> 宏基因组(metagenomic)
<400> 49
Met Gly Ser Ser His His His His His His Ser Ser Pro Ile Ser Pro
1 5 10 15
Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met Asp Gly Pro Lys
20 25 30
Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys Ala Leu Val Glu
35 40 45
Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser Lys Ile Gly Pro
50 55 60
Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys Lys Lys Asp Ser
65 70 75 80
Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu Asn Lys Arg Thr
85 90 95
Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His Pro Ala Gly Leu
100 105 110
Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val Gly Asp Ala Tyr Phe
115 120 125
Ser Val Pro Leu Asp Glu Asp Phe Arg Lys Tyr Thr Ala Phe Thr Ile
130 135 140
Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr Gln Tyr Asn Val
145 150 155 160
Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe Gln Ser Ser Met
165 170 175
Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro Asp Ile Val Ile
180 185 190
Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp Leu Glu Ile Gly
195 200 205
Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln His Leu Leu Arg Trp
210 215 220
Gly Leu Thr Thr Pro Asp Lys Lys His Gln Lys Glu Pro Pro Phe Leu
225 230 235 240
Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr Val Gln Pro Ile
245 250 255
Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp Ile Cys Lys Leu
260 265 270
Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Pro Gly Ile Lys Val
275 280 285
Arg Gln Leu Ser Lys Leu Leu Arg Gly Thr Lys Ala Leu Thr Glu Val
290 295 300
Ile Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala Glu Asn Arg Glu
305 310 315 320
Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp Pro Ser Lys Asp
325 330 335
Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln Trp Thr Tyr Gln
340 345 350
Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly Lys Tyr Ala Arg
355 360 365
Met Arg Gly Ala His Thr Asn Asp Val Lys Gln Leu Thr Glu Ala Val
370 375 380
Gln Lys Ile Thr Thr Glu Ser Ile Val Ile Trp Gly Lys Thr Pro Lys
385 390 395 400
Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Thr Trp Trp Thr Glu
405 410 415
Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe Val Asn Thr Pro
420 425 430
Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu Pro Ile Val Gly
435 440 445
Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn Arg Glu Thr Lys Leu
450 455 460
Gly Lys Ala Gly Tyr Val Thr Asn Arg Gly Arg Gln Lys Val Val Thr
465 470 475 480
Leu Thr Asp Thr Thr Asn Gln Lys Thr Glu Leu Gln Ala Ile Tyr Leu
485 490 495
Ala Leu Gln Asp Ser Gly Leu Glu Val Asn Ile Val Thr Asp Ser Gln
500 505 510
Tyr Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp Gln Ser Glu Ser Glu
515 520 525
Leu Val Asn Gln Ile Ile Glu Gln Leu Ile Lys Lys Glu Lys Val Tyr
530 535 540
Leu Ala Trp Val Pro Ala His Lys Gly Ile Gly Gly Asn Glu Gln Val
545 550 555 560
Asp Lys Leu Val Ser Ala Gly Ile Arg Lys Val Leu
565 570
<210> 50
<211> 126
<212> PRT
<213> 宏基因组(metagenomic)
<400> 50
Met His Val Lys Glu Arg Tyr Lys Asn Phe Leu Asn Gln His Val Gly
1 5 10 15
Pro Asp Met Ser Val Gln Arg Cys Asn Ser Glu Ile Gly Pro Asn Asn
20 25 30
Arg Lys Ile Thr Leu Ser Gly Thr Asp Asn Gly Cys Lys Pro Val Asn
35 40 45
Thr Phe Ile Leu Ala Asn Lys Arg Leu Ile Lys Thr Val Cys Gly Arg
50 55 60
Ala Gly Ser Pro Gln Gly Asn Met Val Arg Ser Asn Gln Pro Phe Pro
65 70 75 80
Val Val Lys Cys Val Leu Asn Asn Gly Glu Arg His Pro Tyr Cys Glu
85 90 95
Tyr Arg Gly Thr Arg Ser Thr Arg Tyr Ile Val Leu Lys Cys Glu Glu
100 105 110
Gly Trp Pro Val His Tyr His Glu Asp Glu Val Asn Val Gly
115 120 125
<210> 51
<211> 130
<212> PRT
<213> 宏基因组(metagenomic)
<400> 51
Met Lys Val Pro Pro Asp Val Asp Pro Arg Tyr Gln Lys Phe Leu Arg
1 5 10 15
Gln His Val Asp Ala Asp Met Ser Val Gln Lys Cys Asp Arg Ala Met
20 25 30
Ser Ile Lys Lys Ile Thr Ala Gly Thr Gly Asn Asp Cys Lys Glu Val
35 40 45
Asn Thr Phe Ile Gln Ala Thr Lys Asp Arg Ile Thr Thr Val Cys Gly
50 55 60
Asp Ala Gly Thr Pro Val Asn Asn Leu Phe Lys Ser Asn Gln Pro Phe
65 70 75 80
Pro Val Val Thr Cys Lys Leu Lys Ser Gly Asn Arg Arg Pro Asn Cys
85 90 95
Gln Tyr Arg Gly Thr Ser Ser Thr Arg Tyr Ile Val Leu Gly Cys Asp
100 105 110
Lys Gly Trp Pro Val His Tyr Asp Glu Gly Ile Ile Asp Val Asn Arg
115 120 125
Ser Gly
130
<210> 52
<211> 280
<212> PRT
<213> 宏基因组(metagenomic)
<400> 52
Met Asn Ile Ile Gly Phe Ser Lys Ala Leu Phe Ser Thr Trp Ile Tyr
1 5 10 15
Tyr Ser Pro Glu Arg Ile Leu Phe Asp Ala Gly Glu Gly Val Ser Thr
20 25 30
Thr Leu Gly Ser Lys Val Tyr Ala Phe Lys Tyr Val Phe Leu Thr His
35 40 45
Gly His Val Asp His Ile Ala Gly Leu Trp Gly Val Val Asn Ile Arg
50 55 60
Asn Asn Gly Met Gly Asp Arg Glu Lys Pro Leu Asp Val Phe Tyr Pro
65 70 75 80
Glu Gly Asn Arg Ala Val Glu Glu Tyr Thr Glu Phe Ile Lys Arg Ala
85 90 95
Asn Pro Asp Leu Arg Phe Ser Phe Asn Val His Pro Leu Lys Glu Gly
100 105 110
Glu Arg Val Phe Leu Arg Asn Ala Gly Gly Phe Lys Arg Tyr Val Gln
115 120 125
Pro Phe Arg Thr Lys His Val Ser Ser Glu Val Ser Phe Gly Tyr His
130 135 140
Ile Phe Glu Val Arg Arg Lys Leu Lys Lys Glu Phe Gln Gly Leu Asp
145 150 155 160
Ser Lys Glu Ile Ser Arg Leu Val Lys Glu Lys Gly Arg Asp Phe Val
165 170 175
Thr Glu Glu Tyr His Lys Lys Val Leu Thr Ile Ser Gly Asp Ser Leu
180 185 190
Ala Leu Asp Pro Glu Glu Ile Arg Gly Thr Glu Leu Leu Ile His Glu
195 200 205
Cys Thr Phe Leu Asp Ala Arg Asp Arg Arg Tyr Lys Asn His Ala Ala
210 215 220
Ile Asp Glu Val Met Glu Ser Val Lys Ala Ala Gly Val Lys Lys Val
225 230 235 240
Ile Leu Tyr His Ile Ser Thr Arg Tyr Ile Arg Gln Leu Lys Ser Val
245 250 255
Ile Lys Lys Tyr Arg Glu Glu Met Pro Asp Val Glu Ile Leu Tyr Met
260 265 270
Asp Pro Arg Lys Val Phe Glu Met
275 280
<210> 53
<211> 107
<212> PRT
<213> 宏基因组(metagenomic)
<400> 53
Met Gln Asp Trp Ala Thr Phe Lys Lys Lys His Leu Thr Asp Thr Trp
1 5 10 15
Asp Val Asp Cys Asp Asn Leu Met Pro Thr Ser Leu Phe Asp Cys Lys
20 25 30
Asp Lys Asn Thr Phe Ile Tyr Ser Leu Pro Gly Pro Val Lys Ala Leu
35 40 45
Cys Arg Gly Val Ile Phe Ser Ala Asp Val Leu Ser Asn Ser Glu Phe
50 55 60
Tyr Leu Ala Glu Cys Asn Val Lys Pro Arg Lys Pro Cys Lys Tyr Lys
65 70 75 80
Leu Lys Lys Ser Ser Asn Arg Ile Cys Ile Arg Cys Glu His Glu Leu
85 90 95
Pro Val His Phe Ala Gly Val Gly Ile Cys Pro
100 105
<210> 54
<211> 106
<212> PRT
<213> 宏基因组(metagenomic)
<400> 54
Met Gln Asn Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Arg
1 5 10 15
Asp Val Lys Cys Asp Ala Glu Met Lys Lys Ala Leu Phe Asp Cys Lys
20 25 30
Gln Lys Asn Thr Phe Ile Tyr Ala Arg Pro Gly Arg Val Gln Ala Leu
35 40 45
Cys Lys Asn Ile Ile Val Ser Lys Asn Val Leu Ser Thr Asp Glu Phe
50 55 60
Tyr Leu Ser Asp Cys Asn Arg Ile Lys Leu Pro Cys His Tyr Lys Leu
65 70 75 80
Lys Lys Ser Ser Asn Thr Ile Cys Ile Thr Cys Glu Asn Lys Leu Pro
85 90 95
Val His Phe Val Ala Val Glu Glu Cys Pro
100 105
<210> 55
<211> 284
<212> PRT
<213> 宏基因组(metagenomic)
<400> 55
Met Val Leu Met Ile Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val
1 5 10 15
Ala Leu Arg Ala Leu Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu
20 25 30
Pro Val Val Leu Leu Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu
35 40 45
Ile Ser Ala Ala Val Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro
50 55 60
Glu Ile Phe Glu Gln Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro
65 70 75 80
Gln Leu Leu Phe Leu Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr
85 90 95
Ser Asp Thr Arg Arg Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu
100 105 110
Glu Ser Ala Ile Asp Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser
115 120 125
Arg Ala Asp Leu Ile Val Asp Thr Ser Glu Met Ser Val His Glu Leu
130 135 140
Ala Glu Met Leu Arg Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu
145 150 155 160
Thr Met Val Phe Glu Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp
165 170 175
Ala Asp Tyr Val Phe Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp
180 185 190
Pro Lys Leu Arg Pro Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe
195 200 205
Leu Asp Arg His Thr Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser
210 215 220
Tyr Leu Glu Leu Trp Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr
225 230 235 240
Leu Thr Val Ala Ile Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr
245 250 255
Ile Ala Glu Gln Leu Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val
260 265 270
Gln Ser Arg His Arg Thr Leu Glu Lys Arg Lys Pro
275 280
<210> 56
<211> 295
<212> PRT
<213> 宏基因组(metagenomic)
<400> 56
Met Trp Ser His Pro Gln Phe Glu Lys Ala Ser Met Val Leu Met Ile
1 5 10 15
Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val Ala Leu Arg Ala Leu
20 25 30
Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu Pro Val Val Leu Leu
35 40 45
Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu Ile Ser Ala Ala Val
50 55 60
Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro Glu Ile Phe Glu Gln
65 70 75 80
Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro Gln Leu Leu Phe Leu
85 90 95
Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr Ser Asp Thr Arg Arg
100 105 110
Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu Glu Ser Ala Ile Asp
115 120 125
Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser Arg Ala Asp Leu Ile
130 135 140
Val Asp Thr Ser Glu Met Ser Val His Glu Leu Ala Glu Met Leu Arg
145 150 155 160
Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu Thr Met Val Phe Glu
165 170 175
Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp Ala Asp Tyr Val Phe
180 185 190
Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp Pro Lys Leu Arg Pro
195 200 205
Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe Leu Asp Arg His Thr
210 215 220
Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser Tyr Leu Glu Leu Trp
225 230 235 240
Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr Leu Thr Val Ala Ile
245 250 255
Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr Ile Ala Glu Gln Leu
260 265 270
Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val Gln Ser Arg His Arg
275 280 285
Thr Leu Glu Lys Arg Lys Pro
290 295
<210> 57
<211> 150
<212> PRT
<213> 宏基因组(metagenomic)
<400> 57
Met Tyr Gln Leu Glu Lys Glu Pro Ile Val Gly Ala Glu Thr Phe Tyr
1 5 10 15
Val Asp Gly Ala Ala Asn Arg Glu Thr Lys Leu Gly Lys Ala Gly Tyr
20 25 30
Val Thr Asn Arg Gly Arg Gln Lys Val Val Thr Leu Thr Asp Thr Thr
35 40 45
Asn Gln Lys Thr Glu Leu Gln Ala Ile Tyr Leu Ala Leu Gln Asp Ser
50 55 60
Gly Leu Glu Val Asn Ile Val Thr Asp Ser Gln Tyr Ala Leu Gly Ile
65 70 75 80
Ile Thr Gln Trp Ile His Asn Trp Lys Lys Arg Gly Trp Lys Thr Ala
85 90 95
Asp Lys Lys Pro Val Lys Asn Val Asp Leu Val Asn Gln Ile Ile Glu
100 105 110
Gln Leu Ile Lys Lys Glu Lys Val Tyr Leu Ala Trp Val Pro Ala His
115 120 125
Lys Gly Ile Gly Gly Asn Glu Gln Val Asp Lys Leu Val Ser Ala Gly
130 135 140
Ile Arg Lys Val Leu Phe
145 150
<210> 58
<211> 157
<212> PRT
<213> 宏基因组(metagenomic)
<400> 58
Pro Asp Leu Thr Asp Gln Pro Ile Pro Asp Ala Asp Tyr Thr Trp Tyr
1 5 10 15
Thr Asp Gly Ser Ser Phe Leu Gln Glu Gly Gln Arg Arg Ala Gly Ala
20 25 30
Ala Val Thr Thr Glu Thr Glu Val Ile Trp Ala Arg Ala Leu Pro Ala
35 40 45
Gly Thr Ser Ala Gln Arg Ala Glu Leu Ile Ala Leu Thr Gln Ala Leu
50 55 60
Lys Met Ala Glu Gly Lys Lys Leu Asn Val Tyr Thr Asp Ser Arg Tyr
65 70 75 80
Ala Phe Ala Thr Ala His Val His Gly Glu Ile Tyr Arg Arg Arg Gly
85 90 95
Leu Leu Thr Ser Glu Gly Arg Glu Ile Lys Asn Lys Asn Glu Ile Leu
100 105 110
Ala Leu Leu Lys Ala Leu Phe Leu Pro Lys Arg Leu Ser Ile Ile His
115 120 125
Cys Pro Gly His Gln Lys Gly Asn Ser Ala Glu Ala Arg Gly Asn Arg
130 135 140
Met Ala Asp Gln Ala Ala Arg Glu Ala Ala Met Lys Ala
145 150 155
<210> 59
<211> 440
<212> PRT
<213> 宏基因组(metagenomic)
<400> 59
Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met
1 5 10 15
Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys
20 25 30
Ala Leu Val Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser
35 40 45
Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys
50 55 60
Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu
65 70 75 80
Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His
85 90 95
Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val Gly
100 105 110
Asp Ala Tyr Phe Ser Val Pro Leu Asp Glu Asp Phe Arg Lys Tyr Thr
115 120 125
Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr
130 135 140
Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe
145 150 155 160
Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro
165 170 175
Asp Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp
180 185 190
Leu Glu Ile Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln His
195 200 205
Leu Leu Arg Trp Gly Leu Thr Thr Pro Asp Lys Lys His Gln Lys Glu
210 215 220
Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr
225 230 235 240
Val Gln Pro Ile Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp
245 250 255
Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Pro
260 265 270
Gly Ile Lys Val Arg Gln Leu Ser Lys Leu Leu Arg Gly Thr Lys Ala
275 280 285
Leu Thr Glu Val Ile Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala
290 295 300
Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp
305 310 315 320
Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln
325 330 335
Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly
340 345 350
Lys Tyr Ala Arg Met Arg Gly Ala His Thr Asn Asp Val Lys Gln Leu
355 360 365
Thr Glu Ala Val Gln Lys Ile Thr Thr Glu Ser Ile Val Ile Trp Gly
370 375 380
Lys Thr Pro Lys Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Thr
385 390 395 400
Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe
405 410 415
Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu
420 425 430
Pro Ile Val Gly Ala Glu Thr Phe
435 440
<210> 60
<211> 105
<212> PRT
<213> 宏基因组(metagenomic)
<400> 60
Gln Asp Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Lys Lys
1 5 10 15
Val Lys Cys Asp Val Glu Met Ala Lys Ala Leu Phe Asp Cys Lys Lys
20 25 30
Thr Asn Thr Phe Ile Tyr Ala Leu Pro Gly Arg Val Lys Ala Leu Cys
35 40 45
Lys Asn Ile Arg Asp Asn Thr Asp Val Leu Ser Arg Asp Ala Phe Leu
50 55 60
Leu Pro Gln Cys Asp Arg Ile Lys Leu Pro Cys His Tyr Lys Leu Ser
65 70 75 80
Ser Ser Thr Asn Thr Ile Cys Ile Thr Cys Val Asn Gln Leu Pro Ile
85 90 95
His Phe Ala Gly Val Gly Ser Cys Pro
100 105
<210> 61
<211> 28
<212> PRT
<213> 宏基因组(metagenomic)
<400> 61
Gln Ser Pro Met Pro Leu Thr Val Ala Ala Ala Ser Pro Glu Leu Ala
1 5 10 15
Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile Val Arg
20 25
<210> 62
<211> 531
<212> PRT
<213> 宏基因组(metagenomic)
<400> 62
Ser Asn Ala Met Ala Ala Ser Asp Gln His Arg Ser Arg Arg His Asp
1 5 10 15
Glu Ser Ser Ser Arg Pro Asn Lys Lys Lys Lys Val Ser Arg Asn Pro
20 25 30
Glu Thr Asn Leu Leu Phe Asn Leu Asn Ser Cys Ser Lys Ser Lys Asp
35 40 45
Leu Ser Ala Ala Leu Ala Leu Tyr Asp Ala Ala Ile Thr Ser Ser Glu
50 55 60
Val Arg Leu Ser Gln Gln His Phe Gln Thr Leu Leu Tyr Leu Cys Ser
65 70 75 80
Ala Ser Ile Thr Asp Ile Ser Leu Gln Tyr Leu Ala Ile Asp Arg Gly
85 90 95
Phe Glu Ile Phe Asp Arg Met Val Ser Ser Gly Ile Ser Pro Asn Glu
100 105 110
Ala Ser Val Thr Ser Val Ala Arg Leu Ala Ala Ala Lys Gly Asn Gly
115 120 125
Asp Tyr Ala Phe Lys Val Val Lys Glu Phe Val Ser Val Gly Gly Val
130 135 140
Ser Ile Pro Arg Leu Arg Thr Tyr Ala Pro Ala Leu Leu Cys Phe Cys
145 150 155 160
Glu Lys Leu Glu Ala Glu Lys Gly Tyr Glu Val Glu Glu His Met Glu
165 170 175
Ala Ala Gly Ile Ala Leu Glu Glu Ala Glu Ile Ser Ala Leu Leu Lys
180 185 190
Val Ser Ala Ala Thr Gly Arg Glu Asn Lys Val Tyr Arg Tyr Leu His
195 200 205
Lys Leu Arg Glu Tyr Val Gly Cys Val Ser Glu Glu Thr Leu Lys Ile
210 215 220
Ile Glu Glu Trp Phe Cys Gly Glu Lys Ala Gly Glu Val Gly Asp Asn
225 230 235 240
Gly Ile Gly Ser Asp Val Gly Met Leu Arg Glu Ala Val Leu Asn Asn
245 250 255
Gly Gly Gly Trp His Gly His Gly Trp Val Gly Glu Gly Lys Trp Thr
260 265 270
Val Lys Lys Gly Asn Val Ser Ser Thr Gly Arg Cys Leu Ser Cys Ser
275 280 285
Glu Gln Leu Ala Cys Val Asp Thr Asn Glu Val Glu Thr Gln Lys Phe
290 295 300
Val Asp Ser Leu Val Ala Leu Ala Met Asp Arg Lys Thr Lys Met Asn
305 310 315 320
Ser Cys Glu Thr Asn Val Val Phe Ser Glu Phe Gln Asp Trp Leu Glu
325 330 335
Lys His Gly Asp Tyr Glu Ala Ile Val Asp Gly Ala Asn Ile Gly Leu
340 345 350
Tyr Gln Gln Asn Phe Val Asp Gly Ser Phe Ser Leu Ser Gln Leu Glu
355 360 365
Ser Val Met Lys Glu Leu Tyr Arg Glu Ser Gly Asn Asn Lys Trp Pro
370 375 380
Leu Ile Leu Leu His Lys Arg Arg Val Lys Thr Leu Leu Glu Asn Pro
385 390 395 400
Thr His Arg Asn Leu Val Glu Glu Trp Ile Ser Asn Gly Val Leu Tyr
405 410 415
Ala Thr Pro Pro Gly Ser Asn Asp Asp Trp Tyr Trp Leu Tyr Ala Ala
420 425 430
Ala Lys Leu Lys Cys Leu Leu Val Thr Asn Asp Glu Met Arg Asp His
435 440 445
Ile Phe Glu Leu Leu Gly Ser Thr Phe Phe Gln Lys Trp Lys Glu Arg
450 455 460
His Gln Val Arg Tyr Thr Phe Val Lys Gly Asn Leu Lys Leu Glu Met
465 470 475 480
Pro Ser Pro Phe Ser Val Val Ile Gln Glu Ser Glu Lys Gly Ser Trp
485 490 495
His Phe Pro Val Ser Cys Glu Asn Asn Glu Glu Ser Ser Arg Thr Trp
500 505 510
Met Cys Ile Ser Arg Gln Ser Ile Leu Asp Ser Pro Lys Ser Asn Gly
515 520 525
Lys Ile Pro
530
<210> 63
<211> 208
<212> PRT
<213> 宏基因组(metagenomic)
<400> 63
Ser Ser Arg Lys Ala Lys Lys Lys Ala Ile Gln Gln Ser Pro Glu Ala
1 5 10 15
Leu Leu Lys Gln Lys Leu Asp Met Cys Ser Lys Lys Gly Asp Val Leu
20 25 30
Glu Ala Leu Arg Leu Tyr Asp Glu Ala Arg Arg Asn Gly Val Gln Leu
35 40 45
Ser Gln Tyr His Tyr Asn Val Leu Leu Tyr Val Cys Ser Leu Ala Ser
50 55 60
Ala Ser Ser Asn Pro Gly Leu Ser Arg Gly Phe Asp Ile Phe Lys Gln
65 70 75 80
Met Ile Val Asp Lys Val Val Pro Asn Glu Ala Thr Phe Thr Asn Gly
85 90 95
Ala Arg Leu Ala Val Ala Lys Asp Asp Pro Glu Met Ala Phe Asp Met
100 105 110
Val Lys Gln Met Lys Ala Phe Gly Ile Gln Pro Arg Leu Arg Ser Tyr
115 120 125
Gly Pro Ala Leu Phe Gly Phe Cys Arg Lys Gly Asp Ala Asp Lys Ala
130 135 140
Tyr Glu Val Asp Ala His Met Val Glu Ser Glu Val Val Pro Glu Glu
145 150 155 160
Pro Glu Leu Ala Ala Leu Leu Lys Val Ser Met Asp Thr Lys Asn Ala
165 170 175
Asp Lys Val Asn Lys Thr Leu Gln Arg Leu Arg Asp Leu Val Arg Gln
180 185 190
Val Ser Lys Ser Thr Gln Asp Met Ile Glu Glu Trp Gln Lys Ser Glu
195 200 205
<210> 64
<211> 6
<212> PRT
<213> 宏基因组(metagenomic)
<400> 64
Ser Ser Thr Ser Ala Ala
1 5
<210> 65
<211> 200
<212> PRT
<213> 宏基因组(metagenomic)
<400> 65
Tyr Asp Tyr Phe Gln Phe Thr Gln Gln Tyr Gln Leu Ala Val Cys Asn
1 5 10 15
Ser Asn Arg Thr Leu Cys Lys Asp Pro Pro Asp Lys Leu Phe Thr Val
20 25 30
His Gly Leu Trp Pro Ser Asn Met Val Gly Pro Asp Pro Ser Lys Cys
35 40 45
Pro Ile Lys Asn Ile Arg Lys Arg Glu Lys Leu Leu Glu His Gln Leu
50 55 60
Glu Ile Ile Trp Pro Asn Val Phe Asp Arg Thr Lys Asn Asn Leu Phe
65 70 75 80
Trp Asp Lys Glu Trp Met Lys His Gly Ser Cys Gly Tyr Pro Thr Ile
85 90 95
Asp Asn Glu Asn His Tyr Phe Glu Thr Val Ile Lys Met Tyr Ile Ser
100 105 110
Lys Lys Gln Asn Val Ser Arg Ile Leu Ser Lys Ala Lys Ile Glu Pro
115 120 125
Asp Gly Lys Lys Arg Ala Leu Leu Asp Ile Glu Asn Ala Ile Arg Asn
130 135 140
Gly Ala Asp Asn Lys Lys Pro Lys Leu Lys Cys Gln Lys Lys Gly Thr
145 150 155 160
Thr Thr Glu Leu Val Glu Ile Thr Leu Cys Ser Asp Lys Ser Gly Glu
165 170 175
His Phe Ile Asp Cys Pro His Pro Phe Glu Pro Ile Ser Pro His Tyr
180 185 190
Cys Pro Thr Asn Asn Ile Lys Tyr
195 200
<210> 66
<211> 217
<212> PRT
<213> 宏基因组(metagenomic)
<400> 66
Tyr Val Glu Phe Ala Gln Asp Phe Asp Phe Phe Tyr Phe Val Gln Gln
1 5 10 15
Trp Pro Ala Ser Tyr Cys Asp Thr Arg Arg Ser Cys Cys Tyr Pro Thr
20 25 30
Thr Gly Lys Pro Asp Glu Asp Phe Ser Ile His Gly Leu Trp Pro Asn
35 40 45
Tyr Glu Asn Gly Lys Trp Pro Gln Asn Cys Asp Arg Glu Ser Ser Leu
50 55 60
Asp Glu Ser Glu Ile Ser Asp Leu Ile Ser Thr Met Glu Lys Asn Trp
65 70 75 80
Pro Ser Leu Ala Cys Pro Ser Ser Asp Gly Val Arg Phe Trp Ser His
85 90 95
Glu Trp Leu Lys His Gly Thr Cys Ser Ala Leu Gly Glu Arg Ala Tyr
100 105 110
Phe Gln Ala Ala Leu Asp Phe Arg Lys Lys Ser Asn Leu Leu Glu Asn
115 120 125
Leu Lys Asn Ala Glu Ile Thr Pro Arg Asn Gly Glu His Tyr Thr Leu
130 135 140
Glu Ser Ile Lys Lys Ala Ile Glu Glu Gly Val Gly His Ser Pro Tyr
145 150 155 160
Ile Glu Cys Asn Val Asp Thr Gln Gly Asn His Gln Ile Tyr Gln Val
165 170 175
Tyr Leu Cys Val Asp Lys Thr Ala Thr Asp Phe Ile Asp Cys Pro Ile
180 185 190
Phe Pro His Gly Arg Gly Cys Gly Ser Lys Ile Glu Phe Pro Pro Phe
195 200 205
Ser Ser Glu Ser Asp His Asp Glu Phe
210 215
<210> 67
<211> 1440
<212> PRT
<213> 宏基因组(metagenomic)
<400> 67
Ser Asn Ala Met Lys Ile Ser Lys Val Arg Glu Glu Asn Arg Gly Ala
1 5 10 15
Lys Leu Thr Val Asn Ala Lys Thr Ala Val Val Ser Glu Asn Arg Ser
20 25 30
Gln Glu Gly Ile Leu Tyr Asn Asp Pro Ser Arg Tyr Gly Lys Ser Arg
35 40 45
Lys Asn Asp Glu Asp Arg Asp Arg Tyr Ile Glu Ser Arg Leu Lys Ser
50 55 60
Ser Gly Lys Leu Tyr Arg Ile Phe Asn Glu Asp Lys Asn Lys Arg Glu
65 70 75 80
Thr Asp Glu Leu Gln Trp Phe Leu Ser Glu Ile Val Lys Lys Ile Asn
85 90 95
Arg Arg Asn Gly Leu Val Leu Ser Asp Met Leu Ser Val Asp Asp Arg
100 105 110
Ala Phe Glu Lys Ala Phe Glu Lys Tyr Ala Glu Leu Ser Tyr Thr Asn
115 120 125
Arg Arg Asn Lys Val Ser Gly Ser Pro Ala Phe Glu Thr Cys Gly Val
130 135 140
Asp Ala Ala Thr Ala Glu Arg Leu Lys Gly Ile Ile Ser Glu Thr Asn
145 150 155 160
Phe Ile Asn Arg Ile Lys Asn Asn Ile Asp Asn Lys Val Ser Glu Asp
165 170 175
Ile Ile Asp Arg Ile Ile Ala Lys Tyr Leu Lys Lys Ser Leu Cys Arg
180 185 190
Glu Arg Val Lys Arg Gly Leu Lys Lys Leu Leu Met Asn Ala Phe Asp
195 200 205
Leu Pro Tyr Ser Asp Pro Asp Ile Asp Val Gln Arg Asp Phe Ile Asp
210 215 220
Tyr Val Leu Glu Asp Phe Tyr His Val Arg Ala Lys Ser Gln Val Ser
225 230 235 240
Arg Ser Ile Lys Asn Met Asn Met Pro Val Gln Pro Glu Gly Asp Gly
245 250 255
Lys Phe Ala Ile Thr Val Ser Lys Gly Gly Thr Glu Ser Gly Asn Lys
260 265 270
Arg Ser Ala Glu Lys Glu Ala Phe Lys Lys Phe Leu Ser Asp Tyr Ala
275 280 285
Ser Leu Asp Glu Arg Val Arg Asp Asp Met Leu Arg Arg Met Arg Arg
290 295 300
Leu Val Val Leu Tyr Phe Tyr Gly Ser Asp Asp Ser Lys Leu Ser Asp
305 310 315 320
Val Asn Glu Lys Phe Asp Val Trp Glu Asp His Ala Ala Arg Arg Val
325 330 335
Asp Asn Arg Glu Phe Ile Lys Leu Pro Leu Glu Asn Lys Leu Ala Asn
340 345 350
Gly Lys Thr Asp Lys Asp Ala Glu Arg Ile Arg Lys Asn Thr Val Lys
355 360 365
Glu Leu Tyr Arg Asn Gln Asn Ile Gly Cys Tyr Arg Gln Ala Val Lys
370 375 380
Ala Val Glu Glu Asp Asn Asn Gly Arg Tyr Phe Asp Asp Lys Met Leu
385 390 395 400
Asn Met Phe Phe Ile His Arg Ile Glu Tyr Gly Val Glu Lys Ile Tyr
405 410 415
Ala Asn Leu Lys Gln Val Thr Glu Phe Lys Ala Arg Thr Gly Tyr Leu
420 425 430
Ser Glu Lys Ile Trp Lys Asp Leu Ile Asn Tyr Ile Ser Ile Lys Tyr
435 440 445
Ile Ala Met Gly Lys Ala Val Tyr Asn Tyr Ala Met Asp Glu Leu Asn
450 455 460
Ala Ser Asp Lys Lys Glu Ile Glu Leu Gly Lys Ile Ser Glu Glu Tyr
465 470 475 480
Leu Ser Gly Ile Ser Ser Phe Asp Tyr Glu Leu Ile Lys Ala Glu Glu
485 490 495
Met Leu Gln Arg Glu Thr Ala Val Tyr Val Ala Phe Ala Ala Arg His
500 505 510
Leu Ser Ser Gln Thr Val Glu Leu Asp Ser Glu Asn Ser Asp Phe Leu
515 520 525
Leu Leu Lys Pro Lys Gly Thr Met Asp Lys Asn Asp Lys Asn Lys Leu
530 535 540
Ala Ser Asn Asn Ile Leu Asn Phe Leu Lys Asp Lys Glu Thr Leu Arg
545 550 555 560
Asp Thr Ile Leu Gln Tyr Phe Gly Gly His Ser Leu Trp Thr Asp Phe
565 570 575
Pro Phe Asp Lys Tyr Leu Ala Gly Gly Lys Asp Asp Val Asp Phe Leu
580 585 590
Thr Asp Leu Lys Asp Val Ile Tyr Ser Met Arg Asn Asp Ser Phe His
595 600 605
Tyr Ala Thr Glu Asn His Asn Asn Gly Lys Trp Asn Lys Glu Leu Ile
610 615 620
Ser Ala Met Phe Glu His Glu Thr Glu Arg Met Thr Val Val Met Lys
625 630 635 640
Asp Lys Phe Tyr Ser Asn Asn Leu Pro Met Phe Tyr Lys Asn Asp Asp
645 650 655
Leu Lys Lys Leu Leu Ile Asp Leu Tyr Lys Asp Asn Val Glu Arg Ala
660 665 670
Ser Gln Val Pro Ser Phe Asn Lys Val Phe Val Arg Lys Asn Phe Pro
675 680 685
Ala Leu Val Arg Asp Lys Asp Asn Leu Gly Ile Glu Leu Asp Leu Lys
690 695 700
Ala Asp Ala Asp Lys Gly Glu Asn Glu Leu Lys Phe Tyr Asn Ala Leu
705 710 715 720
Tyr Tyr Met Phe Lys Glu Ile Tyr Tyr Asn Ala Phe Leu Asn Asp Lys
725 730 735
Asn Val Arg Glu Arg Phe Ile Thr Lys Ala Thr Lys Val Ala Asp Asn
740 745 750
Tyr Asp Arg Asn Lys Glu Arg Asn Leu Lys Asp Arg Ile Lys Ser Ala
755 760 765
Gly Ser Asp Glu Lys Lys Lys Leu Arg Glu Gln Leu Gln Asn Tyr Ile
770 775 780
Ala Glu Asn Asp Phe Gly Gln Arg Ile Lys Asn Ile Val Gln Val Asn
785 790 795 800
Pro Asp Tyr Thr Leu Ala Gln Ile Cys Gln Leu Ile Met Thr Glu Tyr
805 810 815
Asn Gln Gln Asn Asn Gly Cys Met Gln Lys Lys Ser Ala Ala Arg Lys
820 825 830
Asp Ile Asn Lys Asp Ser Tyr Gln His Tyr Lys Met Leu Leu Leu Val
835 840 845
Asn Leu Arg Lys Ala Phe Leu Glu Phe Ile Lys Glu Asn Tyr Ala Phe
850 855 860
Val Leu Lys Pro Tyr Lys His Asp Leu Cys Asp Lys Ala Asp Phe Val
865 870 875 880
Pro Asp Phe Ala Lys Tyr Val Lys Pro Tyr Ala Gly Leu Ile Ser Arg
885 890 895
Val Ala Gly Ser Ser Glu Leu Gln Lys Trp Tyr Ile Val Ser Arg Phe
900 905 910
Leu Ser Pro Ala Gln Ala Asn His Met Leu Gly Phe Leu His Ser Tyr
915 920 925
Lys Gln Tyr Val Trp Asp Ile Tyr Arg Arg Ala Ser Glu Thr Gly Thr
930 935 940
Glu Ile Asn His Ser Ile Ala Glu Asp Lys Ile Ala Gly Val Asp Ile
945 950 955 960
Thr Asp Val Asp Ala Val Ile Asp Leu Ser Val Lys Leu Cys Gly Thr
965 970 975
Ile Ser Ser Glu Ile Ser Asp Tyr Phe Lys Asp Asp Glu Val Tyr Ala
980 985 990
Glu Tyr Ile Ser Ser Tyr Leu Asp Phe Glu Tyr Asp Gly Gly Asn Tyr
995 1000 1005
Lys Asp Ser Leu Asn Arg Phe Cys Asn Ser Asp Ala Val Asn Asp Gln
1010 1015 1020
Lys Val Ala Leu Tyr Tyr Asp Gly Glu His Pro Lys Leu Asn Arg Asn
1025 1030 1035 1040
Ile Ile Leu Ser Lys Leu Tyr Gly Glu Arg Arg Phe Leu Glu Lys Ile
1045 1050 1055
Thr Asp Arg Val Ser Arg Ser Asp Ile Val Glu Tyr Tyr Lys Leu Lys
1060 1065 1070
Lys Glu Thr Ser Gln Tyr Gln Thr Lys Gly Ile Phe Asp Ser Glu Asp
1075 1080 1085
Glu Gln Lys Asn Ile Lys Lys Phe Gln Glu Met Lys Asn Ile Val Glu
1090 1095 1100
Phe Arg Asp Leu Met Asp Tyr Ser Glu Ile Ala Asp Glu Leu Gln Gly
1105 1110 1115 1120
Gln Leu Ile Asn Trp Ile Tyr Leu Arg Glu Arg Asp Leu Met Asn Phe
1125 1130 1135
Gln Leu Gly Tyr His Tyr Ala Cys Leu Asn Asn Asp Ser Asn Lys Gln
1140 1145 1150
Ala Thr Tyr Val Thr Leu Asp Tyr Gln Gly Lys Lys Asn Arg Lys Ile
1155 1160 1165
Asn Gly Ala Ile Leu Tyr Gln Ile Cys Ala Met Tyr Ile Asn Gly Leu
1170 1175 1180
Pro Leu Tyr Tyr Val Asp Lys Asp Ser Ser Glu Trp Thr Val Ser Asp
1185 1190 1195 1200
Gly Lys Glu Ser Thr Gly Ala Lys Ile Gly Glu Phe Tyr Arg Tyr Ala
1205 1210 1215
Lys Ser Phe Glu Asn Thr Ser Asp Cys Tyr Ala Ser Gly Leu Glu Ile
1220 1225 1230
Phe Glu Asn Ile Ser Glu His Asp Asn Ile Thr Glu Leu Arg Asn Tyr
1235 1240 1245
Ile Glu His Phe Arg Tyr Tyr Ser Ser Phe Asp Arg Ser Phe Leu Gly
1250 1255 1260
Ile Tyr Ser Glu Val Phe Asp Arg Phe Phe Thr Tyr Asp Leu Lys Tyr
1265 1270 1275 1280
Arg Lys Asn Val Pro Thr Ile Leu Tyr Asn Ile Leu Leu Gln His Phe
1285 1290 1295
Val Asn Val Arg Phe Glu Phe Val Ser Gly Lys Lys Met Ile Gly Ile
1300 1305 1310
Asp Lys Lys Asp Arg Lys Ile Ala Lys Glu Lys Glu Cys Ala Arg Ile
1315 1320 1325
Thr Ile Arg Glu Lys Asn Gly Val Tyr Ser Glu Gln Phe Thr Tyr Lys
1330 1335 1340
Leu Lys Asn Gly Thr Val Tyr Val Asp Ala Arg Asp Lys Arg Tyr Leu
1345 1350 1355 1360
Gln Ser Ile Ile Arg Leu Leu Phe Tyr Pro Glu Lys Val Asn Met Asp
1365 1370 1375
Glu Met Ile Glu Val Lys Glu Lys Lys Lys Pro Ser Asp Asn Asn Thr
1380 1385 1390
Gly Lys Gly Tyr Ser Lys Arg Asp Arg Gln Gln Asp Arg Lys Glu Tyr
1395 1400 1405
Asp Lys Tyr Lys Glu Lys Lys Lys Lys Glu Gly Asn Phe Leu Ser Gly
1410 1415 1420
Met Gly Gly Asn Ile Asn Trp Asp Glu Ile Asn Ala Gln Leu Lys Asn
1425 1430 1435 1440
<210> 68
<211> 1159
<212> PRT
<213> 宏基因组(metagenomic)
<400> 68
Met Lys Val Thr Lys Val Gly Gly Ile Ser His Lys Lys Tyr Thr Ser
1 5 10 15
Glu Gly Arg Leu Val Lys Ser Glu Ser Glu Glu Asn Arg Thr Asp Glu
20 25 30
Arg Leu Ser Ala Leu Leu Asn Met Arg Leu Asp Met Tyr Ile Lys Asn
35 40 45
Pro Ser Ser Thr Glu Thr Lys Glu Asn Gln Lys Arg Ile Gly Lys Leu
50 55 60
Lys Lys Phe Phe Ser Asn Lys Met Val Tyr Leu Lys Asp Asn Thr Leu
65 70 75 80
Ser Leu Lys Asn Gly Lys Lys Glu Asn Ile Asp Arg Glu Tyr Ser Glu
85 90 95
Thr Asp Ile Leu Glu Ser Asp Val Arg Asp Lys Lys Asn Phe Ala Val
100 105 110
Leu Lys Lys Ile Tyr Leu Asn Glu Asn Val Asn Ser Glu Glu Leu Glu
115 120 125
Val Phe Arg Asn Asp Ile Lys Lys Lys Leu Asn Lys Ile Asn Ser Leu
130 135 140
Lys Tyr Ser Phe Glu Lys Asn Lys Ala Asn Tyr Gln Lys Ile Asn Glu
145 150 155 160
Asn Asn Ile Glu Lys Val Glu Gly Lys Ser Lys Arg Asn Ile Ile Tyr
165 170 175
Asp Tyr Tyr Arg Glu Ser Ala Lys Arg Asp Ala Tyr Val Ser Asn Val
180 185 190
Lys Glu Ala Phe Asp Lys Leu Tyr Lys Glu Glu Asp Ile Ala Lys Leu
195 200 205
Val Leu Glu Ile Glu Asn Leu Thr Lys Leu Glu Lys Tyr Lys Ile Arg
210 215 220
Glu Phe Tyr His Glu Ile Ile Gly Arg Lys Asn Asp Lys Glu Asn Phe
225 230 235 240
Ala Lys Ile Ile Tyr Glu Glu Ile Gln Asn Val Asn Asn Met Lys Glu
245 250 255
Leu Ile Glu Lys Val Pro Asp Met Ser Glu Leu Lys Lys Ser Gln Val
260 265 270
Phe Tyr Lys Tyr Tyr Leu Asp Lys Glu Glu Leu Asn Asp Lys Asn Ile
275 280 285
Lys Tyr Ala Phe Cys His Phe Val Glu Ile Glu Met Ser Gln Leu Leu
290 295 300
Lys Asn Tyr Val Tyr Lys Arg Leu Ser Asn Ile Ser Asn Asp Lys Ile
305 310 315 320
Lys Arg Ile Phe Glu Tyr Gln Asn Leu Lys Lys Leu Ile Glu Asn Lys
325 330 335
Leu Leu Asn Lys Leu Asp Thr Tyr Val Arg Asn Cys Gly Lys Tyr Asn
340 345 350
Tyr Tyr Leu Gln Asp Gly Glu Ile Ala Thr Ser Asp Phe Ile Ala Arg
355 360 365
Asn Arg Gln Asn Glu Ala Phe Leu Arg Asn Ile Ile Gly Val Ser Ser
370 375 380
Val Ala Tyr Phe Ser Leu Arg Asn Ile Leu Glu Thr Glu Asn Glu Asn
385 390 395 400
Asp Ile Thr Gly Arg Met Arg Gly Lys Thr Val Lys Asn Asn Lys Gly
405 410 415
Glu Glu Lys Tyr Val Ser Gly Glu Val Asp Lys Ile Tyr Asn Glu Asn
420 425 430
Lys Lys Asn Glu Val Lys Glu Asn Leu Lys Met Phe Tyr Ser Tyr Asp
435 440 445
Phe Asn Met Asp Asn Lys Asn Glu Ile Glu Asp Phe Phe Ala Asn Ile
450 455 460
Asp Glu Ala Ile Ser Ser Ile Arg His Gly Ile Val His Phe Asn Leu
465 470 475 480
Glu Leu Glu Gly Lys Asp Ile Phe Ala Phe Lys Asn Ile Ala Pro Ser
485 490 495
Glu Ile Ser Lys Lys Met Phe Gln Asn Glu Ile Asn Glu Lys Lys Leu
500 505 510
Lys Leu Lys Ile Phe Arg Gln Leu Asn Ser Ala Asn Val Phe Arg Tyr
515 520 525
Leu Glu Lys Tyr Lys Ile Leu Asn Tyr Leu Lys Arg Thr Arg Phe Glu
530 535 540
Phe Val Asn Lys Asn Ile Pro Phe Val Pro Ser Phe Thr Lys Leu Tyr
545 550 555 560
Ser Arg Ile Asp Asp Leu Lys Asn Ser Leu Gly Ile Tyr Trp Lys Thr
565 570 575
Pro Lys Thr Asn Asp Asp Asn Lys Thr Lys Glu Ile Ile Asp Ala Gln
580 585 590
Ile Tyr Leu Leu Lys Asn Ile Tyr Tyr Gly Glu Phe Leu Asn Tyr Phe
595 600 605
Met Ser Asn Asn Gly Asn Phe Phe Glu Ile Ser Lys Glu Ile Ile Glu
610 615 620
Leu Asn Lys Asn Asp Lys Arg Asn Leu Lys Thr Gly Phe Tyr Lys Leu
625 630 635 640
Gln Lys Phe Glu Asp Ile Gln Glu Lys Ile Pro Lys Glu Tyr Leu Ala
645 650 655
Asn Ile Gln Ser Leu Tyr Met Ile Asn Ala Gly Asn Gln Asp Glu Glu
660 665 670
Glu Lys Asp Thr Tyr Ile Asp Phe Ile Gln Lys Ile Phe Leu Lys Gly
675 680 685
Phe Met Thr Tyr Leu Ala Asn Asn Gly Arg Leu Ser Leu Ile Tyr Ile
690 695 700
Gly Ser Asp Glu Glu Thr Asn Thr Ser Leu Ala Glu Lys Lys Gln Glu
705 710 715 720
Phe Asp Lys Phe Leu Lys Lys Tyr Glu Gln Asn Asn Asn Ile Lys Ile
725 730 735
Pro Tyr Glu Ile Asn Glu Phe Leu Arg Glu Ile Lys Leu Gly Asn Ile
740 745 750
Leu Lys Tyr Thr Glu Arg Leu Asn Met Phe Tyr Leu Ile Leu Lys Leu
755 760 765
Leu Asn His Lys Glu Leu Thr Asn Leu Lys Gly Ser Leu Glu Lys Tyr
770 775 780
Gln Ser Ala Asn Lys Glu Glu Ala Phe Ser Asp Gln Leu Glu Leu Ile
785 790 795 800
Asn Leu Leu Asn Leu Asp Asn Asn Arg Val Thr Glu Asp Phe Glu Leu
805 810 815
Glu Ala Asp Glu Ile Gly Lys Phe Leu Asp Phe Asn Gly Asn Lys Val
820 825 830
Lys Asp Asn Lys Glu Leu Lys Lys Phe Asp Thr Asn Lys Ile Tyr Phe
835 840 845
Asp Gly Glu Asn Ile Ile Lys His Arg Ala Phe Tyr Asn Ile Lys Lys
850 855 860
Tyr Gly Met Leu Asn Leu Leu Glu Lys Ile Ala Asp Lys Ala Gly Tyr
865 870 875 880
Lys Ile Ser Ile Glu Glu Leu Lys Lys Tyr Ser Asn Lys Lys Asn Glu
885 890 895
Ile Glu Lys Asn His Lys Met Gln Glu Asn Leu His Arg Lys Tyr Ala
900 905 910
Arg Pro Arg Lys Asp Glu Lys Phe Thr Asp Glu Asp Tyr Glu Ser Tyr
915 920 925
Lys Gln Ala Ile Glu Asn Ile Glu Glu Tyr Thr His Leu Lys Asn Lys
930 935 940
Val Glu Phe Asn Glu Leu Asn Leu Leu Gln Gly Leu Leu Leu Arg Ile
945 950 955 960
Leu His Arg Leu Val Gly Tyr Thr Ser Ile Trp Glu Arg Asp Leu Arg
965 970 975
Phe Arg Leu Lys Gly Glu Phe Pro Glu Asn Gln Tyr Ile Glu Glu Ile
980 985 990
Phe Asn Phe Glu Asn Lys Lys Asn Val Lys Tyr Lys Gly Gly Gln Ile
995 1000 1005
Val Glu Lys Tyr Ile Lys Phe Tyr Lys Glu Leu His Gln Asn Asp Glu
1010 1015 1020
Val Lys Ile Asn Lys Tyr Ser Ser Ala Asn Ile Lys Val Leu Lys Gln
1025 1030 1035 1040
Glu Lys Lys Asp Leu Tyr Ile Ala Asn Tyr Ile Ala Ala Phe Asn Tyr
1045 1050 1055
Ile Pro His Ala Glu Ile Ser Leu Leu Glu Val Leu Glu Asn Leu Arg
1060 1065 1070
Lys Leu Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Met Lys Ser
1075 1080 1085
Val Val Asp Ile Leu Lys Glu Tyr Gly Phe Val Ala Thr Phe Lys Ile
1090 1095 1100
Gly Ala Asp Lys Lys Ile Gly Ile Gln Thr Leu Glu Ser Glu Lys Ile
1105 1110 1115 1120
Val His Leu Lys Asn Leu Lys Lys Lys Lys Leu Met Thr Asp Arg Asn
1125 1130 1135
Ser Glu Glu Leu Cys Lys Leu Val Lys Ile Met Phe Glu Tyr Lys Met
1140 1145 1150
Glu Glu Lys Lys Ser Glu Asn
1155
<210> 69
<211> 1232
<212> PRT
<213> 宏基因组(metagenomic)
<400> 69
Met Glu Asn Lys Thr Ser Leu Gly Asn Asn Ile Tyr Tyr Asn Pro Phe
1 5 10 15
Lys Pro Gln Asp Lys Ser Tyr Phe Ala Gly Tyr Phe Asn Ala Ala Met
20 25 30
Glu Asn Thr Asp Ser Val Phe Arg Glu Leu Gly Lys Arg Leu Lys Gly
35 40 45
Lys Glu Tyr Thr Ser Glu Asn Phe Phe Asp Ala Ile Phe Lys Glu Asn
50 55 60
Ile Ser Leu Val Glu Tyr Glu Arg Tyr Val Lys Leu Leu Ser Asp Tyr
65 70 75 80
Phe Pro Met Ala Arg Leu Leu Asp Lys Lys Glu Val Pro Ile Lys Glu
85 90 95
Arg Lys Glu Asn Phe Lys Lys Asn Phe Lys Gly Ile Ile Lys Ala Val
100 105 110
Arg Asp Leu Arg Asn Phe Tyr Thr His Lys Glu His Gly Glu Val Glu
115 120 125
Ile Thr Asp Glu Ile Phe Gly Val Leu Asp Glu Met Leu Lys Ser Thr
130 135 140
Val Leu Thr Val Lys Lys Lys Lys Val Lys Thr Asp Lys Thr Lys Glu
145 150 155 160
Ile Leu Lys Lys Ser Ile Glu Lys Gln Leu Asp Ile Leu Cys Gln Lys
165 170 175
Lys Leu Glu Tyr Leu Arg Asp Thr Ala Arg Lys Ile Glu Glu Lys Arg
180 185 190
Arg Asn Gln Arg Glu Arg Gly Glu Lys Glu Leu Val Ala Pro Phe Lys
195 200 205
Tyr Ser Asp Lys Arg Asp Asp Leu Ile Ala Ala Ile Tyr Asn Asp Ala
210 215 220
Phe Asp Val Tyr Ile Asp Lys Lys Lys Asp Ser Leu Lys Glu Ser Ser
225 230 235 240
Lys Ala Lys Tyr Asn Thr Lys Ser Asp Pro Gln Gln Glu Glu Gly Asp
245 250 255
Leu Lys Ile Pro Ile Ser Lys Asn Gly Val Val Phe Leu Leu Ser Leu
260 265 270
Phe Leu Thr Lys Gln Glu Ile His Ala Phe Lys Ser Lys Ile Ala Gly
275 280 285
Phe Lys Ala Thr Val Ile Asp Glu Ala Thr Val Ser Glu Ala Thr Val
290 295 300
Ser His Gly Lys Asn Ser Ile Cys Phe Met Ala Thr His Glu Ile Phe
305 310 315 320
Ser His Leu Ala Tyr Lys Lys Leu Lys Arg Lys Val Arg Thr Ala Glu
325 330 335
Ile Asn Tyr Gly Glu Ala Glu Asn Ala Glu Gln Leu Ser Val Tyr Ala
340 345 350
Lys Glu Thr Leu Met Met Gln Met Leu Asp Glu Leu Ser Lys Val Pro
355 360 365
Asp Val Val Tyr Gln Asn Leu Ser Glu Asp Val Gln Lys Thr Phe Ile
370 375 380
Glu Asp Trp Asn Glu Tyr Leu Lys Glu Asn Asn Gly Asp Val Gly Thr
385 390 395 400
Met Glu Glu Glu Gln Val Ile His Pro Val Ile Arg Lys Arg Tyr Glu
405 410 415
Asp Lys Phe Asn Tyr Phe Ala Ile Arg Phe Leu Asp Glu Phe Ala Gln
420 425 430
Phe Pro Thr Leu Arg Phe Gln Val His Leu Gly Asn Tyr Leu His Asp
435 440 445
Ser Arg Pro Lys Glu Asn Leu Ile Ser Asp Arg Arg Ile Lys Glu Lys
450 455 460
Ile Thr Val Phe Gly Arg Leu Ser Glu Leu Glu His Lys Lys Ala Leu
465 470 475 480
Phe Ile Lys Asn Thr Glu Thr Asn Glu Asp Arg Glu His Tyr Trp Glu
485 490 495
Ile Phe Pro Asn Pro Asn Tyr Asp Phe Pro Lys Glu Asn Ile Ser Val
500 505 510
Asn Asp Lys Asp Phe Pro Ile Ala Gly Ser Ile Leu Asp Arg Glu Lys
515 520 525
Gln Pro Val Ala Gly Lys Ile Gly Ile Lys Val Lys Leu Leu Asn Gln
530 535 540
Gln Tyr Val Ser Glu Val Asp Lys Ala Val Lys Ala His Gln Leu Lys
545 550 555 560
Gln Arg Lys Ala Ser Lys Pro Ser Ile Gln Asn Ile Ile Glu Glu Ile
565 570 575
Val Pro Ile Asn Glu Ser Asn Pro Lys Glu Ala Ile Val Phe Gly Gly
580 585 590
Gln Pro Thr Ala Tyr Leu Ser Met Asn Asp Ile His Ser Ile Leu Tyr
595 600 605
Glu Phe Phe Asp Lys Trp Glu Lys Lys Lys Glu Lys Leu Glu Lys Lys
610 615 620
Gly Glu Lys Glu Leu Arg Lys Glu Ile Gly Lys Glu Leu Glu Lys Lys
625 630 635 640
Ile Val Gly Lys Ile Gln Ala Gln Ile Gln Gln Ile Ile Asp Lys Asp
645 650 655
Thr Asn Ala Lys Ile Leu Lys Pro Tyr Gln Asp Gly Asn Ser Thr Ala
660 665 670
Ile Asp Lys Glu Lys Leu Ile Lys Asp Leu Lys Gln Glu Gln Asn Ile
675 680 685
Leu Gln Lys Leu Lys Asp Glu Gln Thr Val Arg Glu Lys Glu Tyr Asn
690 695 700
Asp Phe Ile Ala Tyr Gln Asp Lys Asn Arg Glu Ile Asn Lys Val Arg
705 710 715 720
Asp Arg Asn His Lys Gln Tyr Leu Lys Asp Asn Leu Lys Arg Lys Tyr
725 730 735
Pro Glu Ala Pro Ala Arg Lys Glu Val Leu Tyr Tyr Arg Glu Lys Gly
740 745 750
Lys Val Ala Val Trp Leu Ala Asn Asp Ile Lys Arg Phe Met Pro Thr
755 760 765
Asp Phe Lys Asn Glu Trp Lys Gly Glu Gln His Ser Leu Leu Gln Lys
770 775 780
Ser Leu Ala Tyr Tyr Glu Gln Cys Lys Glu Glu Leu Lys Asn Leu Leu
785 790 795 800
Pro Glu Lys Val Phe Gln His Leu Pro Phe Lys Leu Gly Gly Tyr Phe
805 810 815
Gln Gln Lys Tyr Leu Tyr Gln Phe Tyr Thr Cys Tyr Leu Asp Lys Arg
820 825 830
Leu Glu Tyr Ile Ser Gly Leu Val Gln Gln Ala Glu Asn Phe Lys Ser
835 840 845
Glu Asn Lys Val Phe Lys Lys Val Glu Asn Glu Cys Phe Lys Phe Leu
850 855 860
Lys Lys Gln Asn Tyr Thr His Lys Glu Leu Asp Ala Arg Val Gln Ser
865 870 875 880
Ile Leu Gly Tyr Pro Ile Phe Leu Glu Arg Gly Phe Met Asp Glu Lys
885 890 895
Pro Thr Ile Ile Lys Gly Lys Thr Phe Lys Gly Asn Glu Ala Leu Phe
900 905 910
Ala Asp Trp Phe Arg Tyr Tyr Lys Glu Tyr Gln Asn Phe Gln Thr Phe
915 920 925
Tyr Asp Thr Glu Asn Tyr Pro Leu Val Glu Leu Glu Lys Lys Gln Ala
930 935 940
Asp Arg Lys Arg Lys Thr Lys Ile Tyr Gln Gln Lys Lys Asn Asp Val
945 950 955 960
Phe Thr Leu Leu Met Ala Lys His Ile Phe Lys Ser Val Phe Lys Gln
965 970 975
Asp Ser Ile Asp Gln Phe Ser Leu Glu Asp Leu Tyr Gln Ser Arg Glu
980 985 990
Glu Arg Leu Gly Asn Gln Glu Arg Ala Arg Gln Thr Gly Glu Arg Asn
995 1000 1005
Thr Asn Tyr Ile Trp Asn Lys Thr Val Asp Leu Lys Leu Cys Asp Gly
1010 1015 1020
Lys Ile Thr Val Glu Asn Val Lys Leu Lys Asn Val Gly Asp Phe Ile
1025 1030 1035 1040
Lys Tyr Glu Tyr Asp Gln Arg Val Gln Ala Phe Leu Lys Tyr Glu Glu
1045 1050 1055
Asn Ile Glu Trp Gln Ala Phe Leu Ile Lys Glu Ser Lys Glu Glu Glu
1060 1065 1070
Asn Tyr Pro Tyr Val Val Glu Arg Glu Ile Glu Gln Tyr Glu Lys Val
1075 1080 1085
Arg Arg Glu Glu Leu Leu Lys Glu Val His Leu Ile Glu Glu Tyr Ile
1090 1095 1100
Leu Glu Lys Val Lys Asp Lys Glu Ile Leu Lys Lys Gly Asp Asn Gln
1105 1110 1115 1120
Asn Phe Lys Tyr Tyr Ile Leu Asn Gly Leu Leu Lys Gln Leu Lys Asn
1125 1130 1135
Glu Asp Val Glu Ser Tyr Lys Val Phe Asn Leu Asn Thr Glu Pro Glu
1140 1145 1150
Asp Val Asn Ile Asn Gln Leu Lys Gln Glu Ala Thr Asp Leu Glu Gln
1155 1160 1165
Lys Ala Phe Val Leu Thr Tyr Ile Ala Asn Lys Phe Ala His Asn Gln
1170 1175 1180
Leu Pro Lys Lys Glu Phe Trp Asp Tyr Cys Gln Glu Lys Tyr Gly Lys
1185 1190 1195 1200
Ile Glu Lys Glu Lys Thr Tyr Ala Glu Tyr Phe Ala Glu Val Phe Lys
1205 1210 1215
Lys Glu Lys Glu Ala Leu Ile Lys Leu Glu His His His His His His
1220 1225 1230
<210> 70
<211> 954
<212> PRT
<213> 宏基因组(metagenomic)
<400> 70
Met Gly Lys Lys Ile His Ala Arg Asp Leu Arg Glu Gln Arg Lys Thr
1 5 10 15
Asp Arg Thr Glu Lys Phe Ala Asp Gln Asn Lys Lys Arg Glu Ala Glu
20 25 30
Arg Ala Val Pro Lys Lys Asp Ala Ala Val Ser Val Lys Ser Val Ser
35 40 45
Ser Val Ser Ser Lys Lys Asp Asn Val Thr Lys Ser Met Ala Lys Ala
50 55 60
Ala Gly Val Lys Ser Val Phe Ala Val Gly Asn Thr Val Tyr Met Thr
65 70 75 80
Ser Phe Gly Arg Gly Asn Asp Ala Val Leu Glu Gln Lys Ile Val Asp
85 90 95
Thr Ser His Glu Pro Leu Asn Ile Asp Asp Pro Ala Tyr Gln Leu Asn
100 105 110
Val Val Thr Met Asn Gly Tyr Ser Val Thr Gly His Arg Gly Glu Thr
115 120 125
Val Ser Ala Val Thr Asp Asn Pro Leu Arg Arg Phe Asn Gly Arg Lys
130 135 140
Lys Asp Glu Pro Glu Gln Ser Val Pro Thr Asp Met Leu Cys Leu Lys
145 150 155 160
Pro Thr Leu Glu Lys Lys Phe Phe Gly Lys Glu Phe Asp Asp Asn Ile
165 170 175
His Ile Gln Leu Ile Tyr Asn Ile Leu Asp Ile Glu Lys Ile Leu Ala
180 185 190
Val Tyr Ser Thr Asn Ala Ile Tyr Ala Leu Asn Asn Met Ser Ala Asp
195 200 205
Glu Asn Ile Glu Asn Ser Asp Phe Phe Met Lys Arg Thr Thr Asp Glu
210 215 220
Thr Phe Asp Asp Phe Glu Lys Lys Lys Glu Ser Thr Asn Ser Arg Glu
225 230 235 240
Lys Ala Asp Phe Asp Ala Phe Glu Lys Phe Ile Gly Asn Tyr Arg Leu
245 250 255
Ala Tyr Phe Ala Asp Ala Phe Tyr Val Asn Lys Lys Asn Pro Lys Gly
260 265 270
Lys Ala Lys Asn Val Leu Arg Glu Asp Lys Glu Leu Tyr Ser Val Leu
275 280 285
Thr Leu Ile Gly Lys Leu Arg His Trp Cys Val His Ser Glu Glu Gly
290 295 300
Arg Ala Glu Phe Trp Leu Tyr Lys Leu Asp Glu Leu Lys Asp Asp Phe
305 310 315 320
Lys Asn Val Leu Asp Val Val Tyr Asn Arg Pro Val Glu Glu Ile Asn
325 330 335
Asn Arg Phe Ile Glu Asn Asn Lys Val Asn Ile Gln Ile Leu Gly Ser
340 345 350
Val Tyr Lys Asn Thr Asp Ile Ala Glu Leu Val Arg Ser Tyr Tyr Glu
355 360 365
Phe Leu Ile Thr Lys Lys Tyr Lys Asn Met Gly Phe Ser Ile Lys Lys
370 375 380
Leu Arg Glu Ser Met Leu Glu Gly Lys Gly Tyr Ala Asp Lys Glu Tyr
385 390 395 400
Asp Ser Val Arg Asn Lys Leu Tyr Gln Met Thr Asp Phe Ile Leu Tyr
405 410 415
Thr Gly Tyr Ile Asn Glu Asp Ser Asp Arg Ala Asp Asp Leu Val Asn
420 425 430
Thr Leu Arg Ser Ser Leu Lys Glu Asp Asp Lys Thr Thr Val Tyr Cys
435 440 445
Lys Glu Ala Asp Tyr Leu Trp Lys Lys Tyr Arg Glu Ser Ile Arg Glu
450 455 460
Val Ala Asp Ala Leu Asp Gly Asp Asn Ile Lys Lys Leu Ser Lys Ser
465 470 475 480
Asn Ile Glu Ile Gln Glu Asp Lys Leu Arg Lys Cys Phe Ile Ser Tyr
485 490 495
Ala Asp Ser Val Ser Glu Phe Thr Lys Leu Ile Tyr Leu Leu Thr Arg
500 505 510
Phe Leu Ser Gly Lys Glu Ile Asn Asp Leu Val Thr Thr Leu Ile Asn
515 520 525
Lys Phe Asp Asn Ile Arg Ser Phe Leu Glu Ile Met Asp Glu Leu Gly
530 535 540
Leu Asp Arg Thr Phe Thr Ala Glu Tyr Ser Phe Phe Glu Gly Ser Thr
545 550 555 560
Lys Tyr Leu Ala Glu Leu Val Glu Leu Asn Ser Phe Val Lys Ser Cys
565 570 575
Ser Phe Asp Ile Asn Ala Lys Arg Thr Met Tyr Arg Asp Ala Leu Asp
580 585 590
Ile Leu Gly Ile Glu Ser Asp Lys Thr Glu Glu Asp Ile Glu Lys Met
595 600 605
Ile Asp Asn Ile Leu Gln Ile Asp Ala Asn Gly Asp Lys Lys Leu Lys
610 615 620
Lys Asn Asn Gly Leu Arg Asn Phe Ile Ala Ser Asn Val Ile Asp Ser
625 630 635 640
Asn Arg Phe Lys Tyr Leu Val Arg Tyr Gly Asn Pro Lys Lys Ile Arg
645 650 655
Glu Thr Ala Lys Cys Lys Pro Ala Val Arg Phe Val Leu Asn Glu Ile
660 665 670
Pro Asp Ala Gln Ile Glu Arg Tyr Tyr Glu Ala Cys Cys Pro Lys Asn
675 680 685
Thr Ala Leu Cys Ser Ala Asn Lys Arg Arg Glu Lys Leu Ala Asp Met
690 695 700
Ile Ala Glu Ile Lys Phe Glu Asn Phe Ser Asp Ala Gly Asn Tyr Gln
705 710 715 720
Lys Ala Asn Val Thr Ser Arg Thr Ser Glu Ala Glu Ile Lys Arg Lys
725 730 735
Asn Gln Ala Ile Ile Arg Leu Tyr Leu Thr Val Met Tyr Ile Met Leu
740 745 750
Lys Asn Leu Val Asn Val Asn Ala Arg Tyr Val Ile Ala Phe His Cys
755 760 765
Val Glu Arg Asp Thr Lys Leu Tyr Ala Glu Ser Gly Leu Glu Val Gly
770 775 780
Asn Ile Glu Lys Asn Lys Thr Asn Leu Thr Met Ala Val Met Gly Val
785 790 795 800
Lys Leu Glu Asn Gly Ile Ile Lys Thr Glu Phe Asp Lys Ser Phe Ala
805 810 815
Glu Asn Ala Ala Asn Arg Tyr Leu Arg Asn Ala Arg Trp Tyr Lys Leu
820 825 830
Ile Leu Asp Asn Leu Lys Lys Ser Glu Arg Ala Val Val Asn Glu Phe
835 840 845
Arg Asn Thr Val Cys His Leu Asn Ala Ile Arg Asn Ile Asn Ile Asn
850 855 860
Ile Lys Glu Ile Lys Glu Val Glu Asn Tyr Phe Ala Leu Tyr His Tyr
865 870 875 880
Leu Ile Gln Lys His Leu Glu Asn Arg Phe Ala Asp Lys Lys Val Glu
885 890 895
Arg Asp Thr Gly Asp Phe Ile Ser Lys Leu Glu Glu His Lys Thr Tyr
900 905 910
Cys Lys Asp Phe Val Lys Ala Tyr Cys Thr Pro Phe Gly Tyr Asn Leu
915 920 925
Val Arg Tyr Lys Asn Leu Thr Ile Asp Gly Leu Phe Asp Lys Asn Tyr
930 935 940
Pro Gly Lys Asp Asp Ser Asp Glu Gln Lys
945 950
<210> 71
<211> 930
<212> PRT
<213> 宏基因组(metagenomic)
<400> 71
Met Ala Lys Lys Asn Lys Met Lys Pro Arg Glu Leu Arg Glu Ala Gln
1 5 10 15
Lys Lys Ala Arg Gln Leu Lys Ala Ala Glu Ile Asn Asn Asn Ala Ala
20 25 30
Pro Ala Ile Ala Ala Met Pro Ala Ala Glu Val Ile Ala Pro Val Ala
35 40 45
Glu Lys Lys Lys Ser Ser Val Lys Ala Ala Gly Met Lys Ser Ile Leu
50 55 60
Val Ser Glu Asn Lys Met Tyr Ile Thr Ser Phe Gly Lys Gly Asn Ser
65 70 75 80
Ala Val Leu Glu Tyr Glu Val Asp Asn Asn Asp Tyr Asn Lys Thr Gln
85 90 95
Leu Ser Ser Lys Asp Asn Ser Asn Ile Glu Leu Gly Asp Val Asn Glu
100 105 110
Val Asn Ile Thr Phe Ser Ser Lys His Gly Phe Gly Ser Gly Val Glu
115 120 125
Ile Asn Thr Ser Asn Pro Thr His Arg Ser Gly Glu Ser Ser Pro Val
130 135 140
Arg Gly Asp Met Leu Gly Leu Lys Ser Glu Leu Glu Lys Arg Phe Phe
145 150 155 160
Gly Lys Thr Phe Asp Asp Asn Ile His Ile Gln Leu Ile Tyr Asn Ile
165 170 175
Leu Asp Ile Glu Lys Ile Leu Ala Val Tyr Val Thr Asn Ile Val Tyr
180 185 190
Ala Leu Asn Asn Met Leu Gly Ile Lys Asp Ser Glu Ser Tyr Asp Asp
195 200 205
Phe Met Gly Tyr Leu Ser Ala Arg Asn Thr Tyr Glu Val Phe Thr His
210 215 220
Pro Asp Lys Ser Asn Leu Ser Asp Lys Val Lys Gly Asn Ile Lys Lys
225 230 235 240
Ser Leu Ser Lys Phe Asn Asp Leu Leu Lys Thr Lys Arg Leu Gly Tyr
245 250 255
Phe Gly Leu Glu Glu Pro Lys Thr Lys Asp Thr Arg Ala Ser Glu Ala
260 265 270
Tyr Lys Lys Arg Val Tyr His Met Leu Ala Ile Val Gly Gln Ile Ala
275 280 285
Gln Cys Val Phe His Asp Lys Ser Gly Ala Lys Arg Phe Asp Leu Tyr
290 295 300
Ser Phe Ile Asn Asn Ile Asp Pro Glu Tyr Arg Asp Thr Leu Asp Tyr
305 310 315 320
Leu Val Glu Glu Arg Leu Lys Ser Ile Asn Lys Asp Phe Ile Glu Gly
325 330 335
Asn Lys Val Asn Ile Ser Leu Leu Ile Asp Met Met Lys Gly Tyr Glu
340 345 350
Ala Asp Asp Ile Ile Arg Leu Tyr Tyr Asp Phe Ile Val Leu Lys Ser
355 360 365
Gln Lys Asn Leu Gly Phe Ser Ile Lys Lys Leu Arg Glu Lys Met Leu
370 375 380
Glu Glu Tyr Gly Phe Arg Phe Lys Asp Lys Gln Tyr Asp Ser Val Arg
385 390 395 400
Ser Lys Met Tyr Lys Leu Met Asp Phe Leu Leu Phe Cys Asn Tyr Tyr
405 410 415
Arg Asn Asp Val Ala Ala Gly Glu Ala Leu Val Arg Lys Leu Arg Phe
420 425 430
Ser Met Thr Asp Asp Glu Lys Glu Gly Ile Tyr Ala Asp Glu Ala Ala
435 440 445
Lys Leu Trp Gly Lys Phe Arg Asn Asp Phe Glu Asn Ile Ala Asp His
450 455 460
Met Asn Gly Asp Val Ile Lys Glu Leu Gly Lys Ala Asp Met Asp Phe
465 470 475 480
Asp Glu Lys Ile Leu Asp Ser Glu Lys Lys Asn Ala Ser Asp Leu Leu
485 490 495
Tyr Phe Ser Lys Met Ile Tyr Met Leu Thr Tyr Phe Leu Asp Gly Lys
500 505 510
Glu Ile Asn Asp Leu Leu Thr Thr Leu Ile Ser Lys Phe Asp Asn Ile
515 520 525
Lys Glu Phe Leu Lys Ile Met Lys Ser Ser Ala Val Asp Val Glu Cys
530 535 540
Glu Leu Thr Ala Gly Tyr Lys Leu Phe Asn Asp Ser Gln Arg Ile Thr
545 550 555 560
Asn Glu Leu Phe Ile Val Lys Asn Ile Ala Ser Met Arg Lys Pro Ala
565 570 575
Ala Ser Ala Lys Leu Thr Met Phe Arg Asp Ala Leu Thr Ile Leu Gly
580 585 590
Ile Asp Asp Asn Ile Thr Asp Asp Arg Ile Ser Glu Ile Leu Lys Leu
595 600 605
Lys Glu Lys Gly Lys Gly Ile His Gly Leu Arg Asn Phe Ile Thr Asn
610 615 620
Asn Val Ile Glu Ser Ser Arg Phe Val Tyr Leu Ile Lys Tyr Ala Asn
625 630 635 640
Ala Gln Lys Ile Arg Glu Val Ala Lys Asn Glu Lys Val Val Met Phe
645 650 655
Val Leu Gly Gly Ile Pro Asp Thr Gln Ile Glu Arg Tyr Tyr Lys Ser
660 665 670
Cys Val Glu Phe Pro Asp Met Asn Ser Ser Leu Glu Ala Lys Arg Ser
675 680 685
Glu Leu Ala Arg Met Ile Lys Asn Ile Ser Phe Asp Asp Phe Lys Asn
690 695 700
Val Lys Gln Gln Ala Lys Gly Arg Glu Asn Val Ala Lys Glu Arg Ala
705 710 715 720
Lys Ala Val Ile Gly Leu Tyr Leu Thr Val Met Tyr Leu Leu Val Lys
725 730 735
Asn Leu Val Asn Val Asn Ala Arg Tyr Val Ile Ala Ile His Cys Leu
740 745 750
Glu Arg Asp Phe Gly Leu Tyr Lys Glu Ile Ile Pro Glu Leu Ala Ser
755 760 765
Lys Asn Leu Lys Asn Asp Tyr Arg Ile Leu Ser Gln Thr Leu Cys Glu
770 775 780
Leu Cys Asp Asp Arg Asn Glu Ser Ser Asn Leu Phe Leu Lys Lys Asn
785 790 795 800
Lys Arg Leu Arg Lys Cys Val Glu Val Asp Ile Asn Asn Ala Asp Ser
805 810 815
Ser Met Thr Arg Lys Tyr Ala Asn Cys Ile Ala His Leu Thr Val Val
820 825 830
Arg Glu Leu Lys Glu Tyr Ile Gly Asp Ile Arg Thr Val Asp Ser Tyr
835 840 845
Phe Ser Ile Tyr His Tyr Val Met Gln Arg Cys Ile Thr Lys Arg Gly
850 855 860
Asp Asp Thr Lys Gln Glu Glu Lys Ile Lys Tyr Glu Asp Asp Leu Leu
865 870 875 880
Lys Asn His Gly Tyr Thr Lys Asp Phe Val Lys Ala Leu Asn Ser Pro
885 890 895
Phe Gly Tyr Asn Ile Pro Arg Phe Lys Asn Leu Ser Ile Glu Gln Leu
900 905 910
Phe Asp Arg Asn Glu Tyr Leu Thr Glu Lys Leu Glu His His His His
915 920 925
His His
930
<210> 72
<211> 1087
<212> PRT
<213> 宏基因组(metagenomic)
<400> 72
Thr Met Arg Ile Thr Lys Val Glu Val Asp Arg Lys Lys Val Leu Ile
1 5 10 15
Ser Arg Asp Lys Asn Gly Gly Lys Leu Val Tyr Glu Asn Glu Met Gln
20 25 30
Asp Asn Thr Glu Gln Ile Met His His Lys Lys Ser Ser Phe Tyr Lys
35 40 45
Ser Val Val Asn Lys Thr Ile Cys Arg Pro Glu Gln Lys Gln Met Lys
50 55 60
Lys Leu Val His Gly Leu Leu Gln Glu Asn Ser Gln Glu Lys Ile Lys
65 70 75 80
Val Ser Asp Val Thr Lys Leu Asn Ile Ser Asn Phe Leu Asn His Arg
85 90 95
Phe Lys Lys Ser Leu Tyr Tyr Phe Pro Glu Asn Ser Pro Asp Lys Ser
100 105 110
Glu Glu Tyr Arg Ile Glu Ile Asn Leu Ser Gln Leu Leu Glu Asp Ser
115 120 125
Leu Lys Lys Gln Gln Gly Thr Phe Ile Cys Trp Glu Ser Phe Ser Lys
130 135 140
Asp Met Glu Leu Tyr Ile Asn Trp Ala Glu Asn Tyr Ile Ser Ser Lys
145 150 155 160
Thr Lys Leu Ile Lys Lys Ser Ile Arg Asn Asn Arg Ile Gln Ser Thr
165 170 175
Glu Ser Arg Ser Gly Gln Leu Met Asp Arg Tyr Met Lys Asp Ile Leu
180 185 190
Asn Lys Asn Lys Pro Phe Asp Ile Gln Ser Val Ser Glu Lys Tyr Gln
195 200 205
Leu Glu Lys Leu Thr Ser Ala Leu Lys Ala Thr Phe Lys Glu Ala Lys
210 215 220
Lys Asn Asp Lys Glu Ile Asn Tyr Lys Leu Lys Ser Thr Leu Gln Asn
225 230 235 240
His Glu Arg Gln Ile Ile Glu Glu Leu Lys Glu Asn Ser Glu Leu Asn
245 250 255
Gln Phe Asn Ile Glu Ile Arg Lys His Leu Glu Thr Tyr Phe Pro Ile
260 265 270
Lys Lys Thr Asn Arg Lys Val Gly Asp Ile Arg Asn Leu Glu Ile Gly
275 280 285
Glu Ile Gln Lys Ile Val Asn His Arg Leu Lys Asn Lys Ile Val Gln
290 295 300
Arg Ile Leu Gln Glu Gly Lys Leu Ala Ser Tyr Glu Ile Glu Ser Thr
305 310 315 320
Val Asn Ser Asn Ser Leu Gln Lys Ile Lys Ile Glu Glu Ala Phe Ala
325 330 335
Leu Lys Phe Ile Asn Ala Cys Leu Phe Ala Ser Asn Asn Leu Arg Asn
340 345 350
Met Val Tyr Pro Val Cys Lys Lys Asp Ile Leu Met Ile Gly Glu Phe
355 360 365
Lys Asn Ser Phe Lys Glu Ile Lys His Lys Lys Phe Ile Arg Gln Trp
370 375 380
Ser Gln Phe Phe Ser Gln Glu Ile Thr Val Asp Asp Ile Glu Leu Ala
385 390 395 400
Ser Trp Gly Leu Arg Gly Ala Ile Ala Pro Ile Arg Asn Glu Ile Ile
405 410 415
His Leu Lys Lys His Ser Trp Lys Lys Phe Phe Asn Asn Pro Thr Phe
420 425 430
Lys Val Lys Lys Ser Lys Ile Ile Asn Gly Lys Thr Lys Asp Val Thr
435 440 445
Ser Glu Phe Leu Tyr Lys Glu Thr Leu Phe Lys Asp Tyr Phe Tyr Ser
450 455 460
Glu Leu Asp Ser Val Pro Glu Leu Ile Ile Asn Lys Met Glu Ser Ser
465 470 475 480
Lys Ile Leu Asp Tyr Tyr Ser Ser Asp Gln Leu Asn Gln Val Phe Thr
485 490 495
Ile Pro Asn Phe Glu Leu Ser Leu Leu Thr Ser Ala Val Pro Phe Ala
500 505 510
Pro Ser Phe Lys Arg Val Tyr Leu Lys Gly Phe Asp Tyr Gln Asn Gln
515 520 525
Asp Glu Ala Gln Pro Asp Tyr Asn Leu Lys Leu Asn Ile Tyr Asn Glu
530 535 540
Lys Ala Phe Asn Ser Glu Ala Phe Gln Ala Gln Tyr Ser Leu Phe Lys
545 550 555 560
Met Val Tyr Tyr Gln Val Phe Leu Pro Gln Phe Thr Thr Asn Asn Asp
565 570 575
Leu Phe Lys Ser Ser Val Asp Phe Ile Leu Thr Leu Asn Lys Glu Arg
580 585 590
Lys Gly Tyr Ala Lys Ala Phe Gln Asp Ile Arg Lys Met Asn Lys Asp
595 600 605
Glu Lys Pro Ser Glu Tyr Met Ser Tyr Ile Gln Ser Gln Leu Met Leu
610 615 620
Tyr Gln Lys Lys Gln Glu Glu Lys Glu Lys Ile Asn His Phe Glu Lys
625 630 635 640
Phe Ile Asn Gln Val Phe Ile Lys Gly Phe Asn Ser Phe Ile Glu Lys
645 650 655
Asn Arg Leu Thr Tyr Ile Cys His Pro Thr Lys Asn Thr Val Pro Glu
660 665 670
Asn Asp Asn Ile Glu Ile Pro Phe His Thr Asp Met Asp Asp Ser Asn
675 680 685
Ile Ala Phe Trp Leu Met Cys Lys Leu Leu Asp Ala Lys Gln Leu Ser
690 695 700
Glu Leu Arg Asn Glu Met Ile Lys Phe Ser Cys Ser Leu Gln Ser Thr
705 710 715 720
Glu Glu Ile Ser Thr Phe Thr Lys Ala Arg Glu Val Ile Gly Leu Ala
725 730 735
Leu Leu Asn Gly Glu Lys Gly Cys Asn Asp Trp Lys Glu Leu Phe Asp
740 745 750
Asp Lys Glu Ala Trp Lys Lys Asn Met Ser Leu Tyr Val Ser Glu Glu
755 760 765
Leu Leu Gln Ser Leu Pro Tyr Thr Gln Glu Asp Gly Gln Thr Pro Val
770 775 780
Ile Asn Arg Ser Ile Asp Leu Val Lys Lys Tyr Gly Thr Glu Thr Ile
785 790 795 800
Leu Glu Lys Leu Phe Ser Ser Ser Asp Asp Tyr Lys Val Ser Ala Lys
805 810 815
Asp Ile Ala Lys Leu His Glu Tyr Asp Val Thr Glu Lys Ile Ala Gln
820 825 830
Gln Glu Ser Leu His Lys Gln Trp Ile Glu Lys Pro Gly Leu Ala Arg
835 840 845
Asp Ser Ala Trp Thr Lys Lys Tyr Gln Asn Val Ile Asn Asp Ile Ser
850 855 860
Asn Tyr Gln Trp Ala Lys Thr Lys Val Glu Leu Thr Gln Val Arg His
865 870 875 880
Leu His Gln Leu Thr Ile Asp Leu Leu Ser Arg Leu Ala Gly Tyr Met
885 890 895
Ser Ile Ala Asp Arg Asp Phe Gln Phe Ser Ser Asn Tyr Ile Leu Glu
900 905 910
Arg Glu Asn Ser Glu Tyr Arg Val Thr Ser Trp Ile Leu Leu Ser Glu
915 920 925
Asn Lys Asn Lys Asn Lys Tyr Asn Asp Tyr Glu Leu Tyr Asn Leu Lys
930 935 940
Asn Ala Ser Ile Lys Val Ser Ser Lys Asn Asp Pro Gln Leu Lys Val
945 950 955 960
Asp Leu Lys Gln Leu Arg Leu Thr Leu Glu Tyr Leu Glu Leu Phe Asp
965 970 975
Asn Arg Leu Lys Glu Lys Arg Asn Asn Ile Ser His Phe Asn Tyr Leu
980 985 990
Asn Gly Gln Leu Gly Asn Ser Ile Leu Glu Leu Phe Asp Asp Ala Arg
995 1000 1005
Asp Val Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys Ser
1010 1015 1020
Leu Lys Glu Ile Leu Ser Ser His Gly Met Glu Val Thr Phe Lys Pro
1025 1030 1035 1040
Leu Tyr Gln Thr Asn His His Leu Lys Ile Asp Lys Leu Gln Pro Lys
1045 1050 1055
Lys Ile His His Leu Gly Glu Lys Ser Thr Val Ser Ser Asn Gln Val
1060 1065 1070
Ser Asn Glu Tyr Cys Gln Leu Val Arg Thr Leu Leu Thr Met Lys
1075 1080 1085
<210> 73
<211> 1126
<212> PRT
<213> 宏基因组(metagenomic)
<400> 73
Met Trp Ile Ser Ile Lys Thr Leu Ile His His Leu Gly Val Leu Phe
1 5 10 15
Phe Cys Asp Tyr Met Tyr Asn Arg Arg Glu Lys Lys Ile Ile Glu Val
20 25 30
Lys Thr Met Arg Ile Thr Lys Val Glu Val Asp Arg Lys Lys Val Leu
35 40 45
Ile Ser Arg Asp Lys Asn Gly Gly Lys Leu Val Tyr Glu Asn Glu Met
50 55 60
Gln Asp Asn Thr Glu Gln Ile Met His His Lys Lys Ser Ser Phe Tyr
65 70 75 80
Lys Ser Val Val Asn Lys Thr Ile Cys Arg Pro Glu Gln Lys Gln Met
85 90 95
Lys Lys Leu Val His Gly Leu Leu Gln Glu Asn Ser Gln Glu Lys Ile
100 105 110
Lys Val Ser Asp Val Thr Lys Leu Asn Ile Ser Asn Phe Leu Asn His
115 120 125
Arg Phe Lys Lys Ser Leu Tyr Tyr Phe Pro Glu Asn Ser Pro Asp Lys
130 135 140
Ser Glu Glu Tyr Arg Ile Glu Ile Asn Leu Ser Gln Leu Leu Glu Asp
145 150 155 160
Ser Leu Lys Lys Gln Gln Gly Thr Phe Ile Cys Trp Glu Ser Phe Ser
165 170 175
Lys Asp Met Glu Leu Tyr Ile Asn Trp Ala Glu Asn Tyr Ile Ser Ser
180 185 190
Lys Thr Lys Leu Ile Lys Lys Ser Ile Arg Asn Asn Arg Ile Gln Ser
195 200 205
Thr Glu Ser Arg Ser Gly Gln Leu Met Asp Arg Tyr Met Lys Asp Ile
210 215 220
Leu Asn Lys Asn Lys Pro Phe Asp Ile Gln Ser Val Ser Glu Lys Tyr
225 230 235 240
Gln Leu Glu Lys Leu Thr Ser Ala Leu Lys Ala Thr Phe Lys Glu Ala
245 250 255
Lys Lys Asn Asp Lys Glu Ile Asn Tyr Lys Leu Lys Ser Thr Leu Gln
260 265 270
Asn His Glu Arg Gln Ile Ile Glu Glu Leu Lys Glu Asn Ser Glu Leu
275 280 285
Asn Gln Phe Asn Ile Glu Ile Arg Lys His Leu Glu Thr Tyr Phe Pro
290 295 300
Ile Lys Lys Thr Asn Arg Lys Val Gly Asp Ile Arg Asn Leu Glu Ile
305 310 315 320
Gly Glu Ile Gln Lys Ile Val Asn His Arg Leu Lys Asn Lys Ile Val
325 330 335
Gln Arg Ile Leu Gln Glu Gly Lys Leu Ala Ser Tyr Glu Ile Glu Ser
340 345 350
Thr Val Asn Ser Asn Ser Leu Gln Lys Ile Lys Ile Glu Glu Ala Phe
355 360 365
Ala Leu Lys Phe Ile Asn Ala Cys Leu Phe Ala Ser Asn Asn Leu Arg
370 375 380
Asn Met Val Tyr Pro Val Cys Lys Lys Asp Ile Leu Met Ile Gly Glu
385 390 395 400
Phe Lys Asn Ser Phe Lys Glu Ile Lys His Lys Lys Phe Ile Arg Gln
405 410 415
Trp Ser Gln Phe Phe Ser Gln Glu Ile Thr Val Asp Asp Ile Glu Leu
420 425 430
Ala Ser Trp Gly Leu Arg Gly Ala Ile Ala Pro Ile Arg Asn Glu Ile
435 440 445
Ile His Leu Lys Lys His Ser Trp Lys Lys Phe Phe Asn Asn Pro Thr
450 455 460
Phe Lys Val Lys Lys Ser Lys Ile Ile Asn Gly Lys Thr Lys Asp Val
465 470 475 480
Thr Ser Glu Phe Leu Tyr Lys Glu Thr Leu Phe Lys Asp Tyr Phe Tyr
485 490 495
Ser Glu Leu Asp Ser Val Pro Glu Leu Ile Ile Asn Lys Met Glu Ser
500 505 510
Ser Lys Ile Leu Asp Tyr Tyr Ser Ser Asp Gln Leu Asn Gln Val Phe
515 520 525
Thr Ile Pro Asn Phe Glu Leu Ser Leu Leu Thr Ser Ala Val Pro Phe
530 535 540
Ala Pro Ser Phe Lys Arg Val Tyr Leu Lys Gly Phe Asp Tyr Gln Asn
545 550 555 560
Gln Asp Glu Ala Gln Pro Asp Tyr Asn Leu Lys Leu Asn Ile Tyr Asn
565 570 575
Glu Lys Ala Phe Asn Ser Glu Ala Phe Gln Ala Gln Tyr Ser Leu Phe
580 585 590
Lys Met Val Tyr Tyr Gln Val Phe Leu Pro Gln Phe Thr Thr Asn Asn
595 600 605
Asp Leu Phe Lys Ser Ser Val Asp Phe Ile Leu Thr Leu Asn Lys Glu
610 615 620
Arg Lys Gly Tyr Ala Lys Ala Phe Gln Asp Ile Arg Lys Met Asn Lys
625 630 635 640
Asp Glu Lys Pro Ser Glu Tyr Met Ser Tyr Ile Gln Ser Gln Leu Met
645 650 655
Leu Tyr Gln Lys Lys Gln Glu Glu Lys Glu Lys Ile Asn His Phe Glu
660 665 670
Lys Phe Ile Asn Gln Val Phe Ile Lys Gly Phe Asn Ser Phe Ile Glu
675 680 685
Lys Asn Arg Leu Thr Tyr Ile Cys His Pro Thr Lys Asn Thr Val Pro
690 695 700
Glu Asn Asp Asn Ile Glu Ile Pro Phe His Thr Asp Met Asp Asp Ser
705 710 715 720
Asn Ile Ala Phe Trp Leu Met Cys Lys Leu Leu Asp Ala Lys Gln Leu
725 730 735
Ser Glu Leu Arg Asn Glu Met Ile Lys Phe Ser Cys Ser Leu Gln Ser
740 745 750
Thr Glu Glu Ile Ser Thr Phe Thr Lys Ala Arg Glu Val Ile Gly Leu
755 760 765
Ala Leu Leu Asn Gly Glu Lys Gly Cys Asn Asp Trp Lys Glu Leu Phe
770 775 780
Asp Asp Lys Glu Ala Trp Lys Lys Asn Met Ser Leu Tyr Val Ser Glu
785 790 795 800
Glu Leu Leu Gln Ser Leu Pro Tyr Thr Gln Glu Asp Gly Gln Thr Pro
805 810 815
Val Ile Asn Arg Ser Ile Asp Leu Val Lys Lys Tyr Gly Thr Glu Thr
820 825 830
Ile Leu Glu Lys Leu Phe Ser Ser Ser Asp Asp Tyr Lys Val Ser Ala
835 840 845
Lys Asp Ile Ala Lys Leu His Glu Tyr Asp Val Thr Glu Lys Ile Ala
850 855 860
Gln Gln Glu Ser Leu His Lys Gln Trp Ile Glu Lys Pro Gly Leu Ala
865 870 875 880
Arg Asp Ser Ala Trp Thr Lys Lys Tyr Gln Asn Val Ile Asn Asp Ile
885 890 895
Ser Asn Tyr Gln Trp Ala Lys Thr Lys Val Glu Leu Thr Gln Val Arg
900 905 910
His Leu His Gln Leu Thr Ile Asp Leu Leu Ser Arg Leu Ala Gly Tyr
915 920 925
Met Ser Ile Ala Asp Arg Asp Phe Gln Phe Ser Ser Asn Tyr Ile Leu
930 935 940
Glu Arg Glu Asn Ser Glu Tyr Arg Val Thr Ser Trp Ile Leu Leu Ser
945 950 955 960
Glu Asn Lys Asn Lys Asn Lys Tyr Asn Asp Tyr Glu Leu Tyr Asn Leu
965 970 975
Lys Asn Ala Ser Ile Lys Val Ser Ser Lys Asn Asp Pro Gln Leu Lys
980 985 990
Val Asp Leu Lys Gln Leu Arg Leu Thr Leu Glu Tyr Leu Glu Leu Phe
995 1000 1005
Asp Asn Arg Leu Lys Glu Lys Arg Asn Asn Ile Ser His Phe Asn Tyr
1010 1015 1020
Leu Asn Gly Gln Leu Gly Asn Ser Ile Leu Glu Leu Phe Asp Asp Ala
1025 1030 1035 1040
Arg Asp Val Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys
1045 1050 1055
Ser Leu Lys Glu Ile Leu Ser Ser His Gly Met Glu Val Thr Phe Lys
1060 1065 1070
Pro Leu Tyr Gln Thr Asn His His Leu Lys Ile Asp Lys Leu Gln Pro
1075 1080 1085
Lys Lys Ile His His Leu Gly Glu Lys Ser Thr Val Ser Ser Asn Gln
1090 1095 1100
Val Ser Asn Glu Tyr Cys Gln Leu Val Arg Thr Leu Leu Thr Met Lys
1105 1110 1115 1120
His His His His His His
1125
<210> 74
<211> 1391
<212> PRT
<213> 宏基因组(metagenomic)
<400> 74
Gly Ser Met Gly Asn Leu Phe Gly His Lys Arg Trp Tyr Glu Val Arg
1 5 10 15
Asp Lys Lys Asp Phe Lys Ile Lys Arg Lys Val Lys Val Lys Arg Asn
20 25 30
Tyr Asp Gly Asn Lys Tyr Ile Leu Asn Ile Asn Glu Asn Asn Asn Lys
35 40 45
Glu Lys Ile Asp Asn Asn Lys Phe Ile Arg Lys Tyr Ile Asn Tyr Lys
50 55 60
Lys Asn Asp Asn Ile Leu Lys Glu Phe Thr Arg Lys Phe His Ala Gly
65 70 75 80
Asn Ile Leu Phe Lys Leu Lys Gly Lys Glu Gly Ile Ile Arg Ile Glu
85 90 95
Asn Asn Asp Asp Phe Leu Glu Thr Glu Glu Val Val Leu Tyr Ile Glu
100 105 110
Ala Tyr Gly Lys Ser Glu Lys Leu Lys Ala Leu Gly Ile Thr Lys Lys
115 120 125
Lys Ile Ile Asp Glu Ala Ile Arg Gln Gly Ile Thr Lys Asp Asp Lys
130 135 140
Lys Ile Glu Ile Lys Arg Gln Glu Asn Glu Glu Glu Ile Glu Ile Asp
145 150 155 160
Ile Arg Asp Glu Tyr Thr Asn Lys Thr Leu Asn Asp Cys Ser Ile Ile
165 170 175
Leu Arg Ile Ile Glu Asn Asp Glu Leu Glu Thr Lys Lys Ser Ile Tyr
180 185 190
Glu Ile Phe Lys Asn Ile Asn Met Ser Leu Tyr Lys Ile Ile Glu Lys
195 200 205
Ile Ile Glu Asn Glu Thr Glu Lys Val Phe Glu Asn Arg Tyr Tyr Glu
210 215 220
Glu His Leu Arg Glu Lys Leu Leu Lys Asp Asp Lys Ile Asp Val Ile
225 230 235 240
Leu Thr Asn Phe Met Glu Ile Arg Glu Lys Ile Lys Ser Asn Leu Glu
245 250 255
Ile Leu Gly Phe Val Lys Phe Tyr Leu Asn Val Gly Gly Asp Lys Lys
260 265 270
Lys Ser Lys Asn Lys Lys Met Leu Val Glu Lys Ile Leu Asn Ile Asn
275 280 285
Val Asp Leu Thr Val Glu Asp Ile Ala Asp Phe Val Ile Lys Glu Leu
290 295 300
Glu Phe Trp Asn Ile Thr Lys Arg Ile Glu Lys Val Lys Lys Val Asn
305 310 315 320
Asn Glu Phe Leu Glu Lys Arg Arg Asn Arg Thr Tyr Ile Lys Ser Tyr
325 330 335
Val Leu Leu Asp Lys His Glu Lys Phe Lys Ile Glu Arg Glu Asn Lys
340 345 350
Lys Asp Lys Ile Val Lys Phe Phe Val Glu Asn Ile Lys Asn Asn Ser
355 360 365
Ile Lys Glu Lys Ile Glu Lys Ile Leu Ala Glu Phe Lys Ile Asp Glu
370 375 380
Leu Ile Lys Lys Leu Glu Lys Glu Leu Lys Lys Gly Asn Cys Asp Thr
385 390 395 400
Glu Ile Phe Gly Ile Phe Lys Lys His Tyr Lys Val Asn Phe Asp Ser
405 410 415
Lys Lys Phe Ser Lys Lys Ser Asp Glu Glu Lys Glu Leu Tyr Lys Ile
420 425 430
Ile Tyr Arg Tyr Leu Lys Gly Arg Ile Glu Lys Ile Leu Val Asn Glu
435 440 445
Gln Lys Val Arg Leu Lys Lys Met Glu Lys Ile Glu Ile Glu Lys Ile
450 455 460
Leu Asn Glu Ser Ile Leu Ser Glu Lys Ile Leu Lys Arg Val Lys Gln
465 470 475 480
Tyr Thr Leu Glu His Ile Met Tyr Leu Gly Lys Leu Arg His Asn Asp
485 490 495
Ile Asp Met Thr Thr Val Asn Thr Asp Asp Phe Ser Arg Leu His Ala
500 505 510
Lys Glu Glu Leu Asp Leu Glu Leu Ile Thr Phe Phe Ala Ser Thr Asn
515 520 525
Met Glu Leu Asn Lys Ile Phe Ser Arg Glu Asn Ile Asn Asn Asp Glu
530 535 540
Asn Ile Asp Phe Phe Gly Gly Asp Arg Glu Lys Asn Tyr Val Leu Asp
545 550 555 560
Lys Lys Ile Leu Asn Ser Lys Ile Lys Ile Ile Arg Asp Leu Asp Phe
565 570 575
Ile Asp Asn Lys Asn Asn Ile Thr Asn Asn Phe Ile Arg Lys Phe Thr
580 585 590
Lys Ile Gly Thr Asn Glu Arg Asn Arg Ile Leu His Ala Ile Ser Lys
595 600 605
Glu Arg Asp Leu Gln Gly Thr Gln Asp Asp Tyr Asn Lys Val Ile Asn
610 615 620
Ile Ile Gln Asn Leu Lys Ile Ser Asp Glu Glu Val Ser Lys Ala Leu
625 630 635 640
Asn Leu Asp Val Val Phe Lys Asp Lys Lys Asn Ile Ile Thr Lys Ile
645 650 655
Asn Asp Ile Lys Ile Ser Glu Glu Asn Asn Asn Asp Ile Lys Tyr Leu
660 665 670
Pro Ser Phe Ser Lys Val Leu Pro Glu Ile Leu Asn Leu Tyr Arg Asn
675 680 685
Asn Pro Lys Asn Glu Pro Phe Asp Thr Ile Glu Thr Glu Lys Ile Val
690 695 700
Leu Asn Ala Leu Ile Tyr Val Asn Lys Glu Leu Tyr Lys Lys Leu Ile
705 710 715 720
Leu Glu Asp Asp Leu Glu Glu Asn Glu Ser Lys Asn Ile Phe Leu Gln
725 730 735
Glu Leu Lys Lys Thr Leu Gly Asn Ile Asp Glu Ile Asp Glu Asn Ile
740 745 750
Ile Glu Asn Tyr Tyr Lys Asn Ala Gln Ile Ser Ala Ser Lys Gly Asn
755 760 765
Asn Lys Ala Ile Lys Lys Tyr Gln Lys Lys Val Ile Glu Cys Tyr Ile
770 775 780
Gly Tyr Leu Arg Lys Asn Tyr Glu Glu Leu Phe Asp Phe Ser Asp Phe
785 790 795 800
Lys Met Asn Ile Gln Glu Ile Lys Lys Gln Ile Lys Asp Ile Asn Asp
805 810 815
Asn Lys Thr Tyr Glu Arg Ile Thr Val Lys Thr Ser Asp Lys Thr Ile
820 825 830
Val Ile Asn Asp Asp Phe Glu Tyr Ile Ile Ser Ile Phe Ala Leu Leu
835 840 845
Asn Ser Asn Ala Val Ile Asn Lys Ile Arg Asn Arg Phe Phe Ala Thr
850 855 860
Ser Val Trp Leu Asn Thr Ser Glu Tyr Gln Asn Ile Ile Asp Ile Leu
865 870 875 880
Asp Glu Ile Met Gln Leu Asn Thr Leu Arg Asn Glu Cys Ile Thr Glu
885 890 895
Asn Trp Asn Leu Asn Leu Glu Glu Phe Ile Gln Lys Met Lys Glu Ile
900 905 910
Glu Lys Asp Phe Asp Asp Phe Lys Ile Gln Thr Lys Lys Glu Ile Phe
915 920 925
Asn Asn Tyr Tyr Glu Asp Ile Lys Asn Asn Ile Leu Thr Glu Phe Lys
930 935 940
Asp Asp Ile Asn Gly Cys Asp Val Leu Glu Lys Lys Leu Glu Lys Ile
945 950 955 960
Val Ile Phe Asp Asp Glu Thr Lys Phe Glu Ile Asp Lys Lys Ser Asn
965 970 975
Ile Leu Gln Asp Glu Gln Arg Lys Leu Ser Asn Ile Asn Lys Lys Asp
980 985 990
Leu Lys Lys Lys Val Asp Gln Tyr Ile Lys Asp Lys Asp Gln Glu Ile
995 1000 1005
Lys Ser Lys Ile Leu Cys Arg Ile Ile Phe Asn Ser Asp Phe Leu Lys
1010 1015 1020
Lys Tyr Lys Lys Glu Ile Asp Asn Leu Ile Glu Asp Met Glu Ser Glu
1025 1030 1035 1040
Asn Glu Asn Lys Phe Gln Glu Ile Tyr Tyr Pro Lys Glu Arg Lys Asn
1045 1050 1055
Glu Leu Tyr Ile Tyr Lys Lys Asn Leu Phe Leu Asn Ile Gly Asn Pro
1060 1065 1070
Asn Phe Asp Lys Ile Tyr Gly Leu Ile Ser Asn Asp Ile Lys Met Ala
1075 1080 1085
Asp Ala Lys Phe Leu Phe Asn Ile Asp Gly Lys Asn Ile Arg Lys Asn
1090 1095 1100
Lys Ile Ser Glu Ile Asp Ala Ile Leu Lys Asn Leu Asn Asp Lys Leu
1105 1110 1115 1120
Asn Gly Tyr Ser Lys Glu Tyr Lys Glu Lys Tyr Ile Lys Lys Leu Lys
1125 1130 1135
Glu Asn Asp Asp Phe Phe Ala Lys Asn Ile Gln Asn Lys Asn Tyr Lys
1140 1145 1150
Ser Phe Glu Lys Asp Tyr Asn Arg Val Ser Glu Tyr Lys Lys Ile Arg
1155 1160 1165
Asp Leu Val Glu Phe Asn Tyr Leu Asn Lys Ile Glu Ser Tyr Leu Ile
1170 1175 1180
Asp Ile Asn Trp Lys Leu Ala Ile Gln Met Ala Arg Phe Glu Arg Asp
1185 1190 1195 1200
Met His Tyr Ile Val Asn Gly Leu Arg Glu Leu Gly Ile Ile Lys Leu
1205 1210 1215
Ser Gly Tyr Asn Thr Gly Ile Ser Arg Ala Tyr Pro Lys Arg Asn Gly
1220 1225 1230
Ser Asp Gly Phe Tyr Thr Thr Thr Ala Tyr Tyr Lys Phe Phe Asp Glu
1235 1240 1245
Glu Ser Tyr Lys Lys Phe Glu Lys Ile Cys Tyr Gly Phe Gly Ile Asp
1250 1255 1260
Leu Ser Glu Asn Ser Glu Ile Asn Lys Pro Glu Asn Glu Ser Ile Arg
1265 1270 1275 1280
Asn Tyr Ile Ser His Phe Tyr Ile Val Arg Asn Pro Phe Ala Asp Tyr
1285 1290 1295
Ser Ile Ala Glu Gln Ile Asp Arg Val Ser Asn Leu Leu Ser Tyr Ser
1300 1305 1310
Thr Arg Tyr Asn Asn Ser Thr Tyr Ala Ser Val Phe Glu Val Phe Lys
1315 1320 1325
Lys Asp Val Asn Leu Asp Tyr Asp Glu Leu Lys Lys Lys Phe Lys Leu
1330 1335 1340
Ile Gly Asn Asn Asp Ile Leu Glu Arg Leu Met Lys Pro Lys Lys Val
1345 1350 1355 1360
Ser Val Leu Glu Leu Glu Ser Tyr Asn Ser Asp Tyr Ile Lys Asn Leu
1365 1370 1375
Ile Ile Glu Leu Leu Thr Lys Ile Glu Asn Thr Asn Asp Thr Leu
1380 1385 1390
<210> 75
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
gctgtgatag gccccgattt gtggggtagt aacagc 36
<210> 76
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
gctggagcag cccccgattt gtggggtaat cacagc 36
<210> 77
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
ggctggagca gccctcgatt tgcagggtaa tcacagc 37
<210> 78
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
gctggagcag ccctcgattt gcagggtaat cacagc 36
<210> 79
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
gctggagcag cctcggattt gcggggtgat tacagc 36
<210> 80
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
gctggagtag cccccgattt gtggggtgat tacagc 36
<210> 81
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
gctgaagaag cctccgattt gtggggtgat tacagc 36
<210> 82
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
gctgtgatgg acctcgattt gtggggtagt aacagct 37
<210> 83
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
gctggagcag cccccgattt gtggggtaat cacagc 36
<210> 84
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
gttgtggttt gattaaagat tagagaacac gatatt 36
<210> 85
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
gttgtgactg ctcttatttt gaagggtaaa cacaac 36
<210> 86
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
ctttccattc cactatggtg caattttggg 30
<210> 87
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
atttccattc cactatggtg caattttggg 30
<210> 88
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
gagtccagat gacccccaga ttgaggggga ctaca 35
<210> 89
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
gctgtactca cccttcaaat aaagggcttt tacagc 36
<210> 90
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
gtcttagttc ccctttatgt ggggataatt tcaac 35
<210> 91
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
gttgaaatta tccccacata aaggggaact aagac 35
<210> 92
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
gttggagcag cccccgtttt gtggggtaat cacaac 36
<210> 93
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
gaactacacc cgtgcaaaat tgcaggggtc taaaac 36
<210> 94
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
tggtatagca ctctactgtt tgacagatgt ctaaaac 37
<210> 95
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
gttttagaca tctgtcaaac agtagagtgc tatacca 37
<210> 96
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
gttgtaactg ctctctgttt agagggtata aacaac 36
<210> 97
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
tgttgtaact gctcttagtt tgaagggtaa aaacaac 37
<210> 98
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
attaaaataa gcacgtttcg tgatggaaat 30
<210> 99
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
gagatagacc cttgttaact cgtaaggttc tgtgact 37
<210> 100
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
gtctggccgg gtgccgtcgg gtgcctggcc 30
<210> 101
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
gtttccgcgg cgaattcgcc gcggctccat tgaagc 36
<210> 102
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
gttacaacag ccccatagat tcgtggggtt ctgaaac 37
<210> 103
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
gttgtaactg cccttatttt gaagggtaaa aacaac 36
<210> 104
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
gctggagcag ccctcgattt gctgggtaat cacagc 36
<210> 105
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
gctgtgatta cccagcaaat cgagggctgc tccagc 36
<210> 106
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
tcctcgaagt tcatcacgcg ctcccacttg 30
<210> 107
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
caagtgggag cgcgtgatga acttcgagga 30
<210> 108
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 109
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60
cctacttgaa gctgtccttc ccc 83
<210> 110
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60
tgcttcacgt aggccttgga gccgtacatg 90
<210> 111
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60
ccatgtacgg ctccaaggcc tac 83
<210> 112
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60
tcctcgaagt tcatcacgcg ctcccacttg 90
<210> 113
<211> 81
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60
ccaagtggga gcgcgtgatg a 81
<210> 114
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
cagcgaagcg catgaactcc ttgatgatgg ccat 34
<210> 115
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
cagcatggcc atcatcaagg agttcatgcg cttc 34
<210> 116
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
cagcagccgt acatgaactg aggggacagg atgt 34
<210> 117
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
cagcacatcc tgtcccctca gttcatgtac ggct 34
<210> 118
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
cagcttcatc acgcgctccc acttgaagcc ctcg 34
<210> 119
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
cagccgaggg cttcaagtgg gagcgcgtga tgaa 34
<210> 120
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
cagcggtgcc gcgcagcttc accttgtaga tgaa 34
<210> 121
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
cagcttcatc tacaaggtga agctgcgcgg cacc 34
<210> 122
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
tcttgtggaa aggacgaaac accggctgga gcagcccccg atttgtgggg taatcacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 123
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
cctaatggat cctagtacaa aaaaagctgt gattacccca caaatcgggg gctgctccag 60
cctacttgaa gctgtccttc ccc 83
<210> 124
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
cagcggtgcc gcgcagcttc accttgtaga tgaa 34
<210> 125
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
cagcttcatc tacaaggtga agctgcgcgg cacc 34
<210> 126
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
cagcgaagcg catgaactcc ttgatgatgg ccat 34
<210> 127
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
cagcatggcc atcatcaagg agttcatgcg cttc 34
<210> 128
<211> 91
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
tcttgtggaa aggacgaaac accgggctgg agcagccctc gatttgcagg gtaatcacag 60
caagccctcg gggaaggaca gcttcaagta g 91
<210> 129
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
cctaatggat cctagtacaa aaaaagctgt gattaccctg caaatcgagg gctgctccag 60
ccctacttga agctgtcctt cccc 84
<210> 130
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
tcttgtggaa aggacgaaac accggctgga gcagccctcg atttgcaggg taatcacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 131
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
cctaatggat cctagtacaa aaaaagctgt gattaccctg caaatcgagg gctgctccag 60
cctacttgaa gctgtccttc ccc 83
<210> 132
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
tcttgtggaa aggacgaaac accggctgga gcagcctcgg atttgcgggg tgattacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 133
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
cctaatggat cctagtacaa aaaaagctgt aatcaccccg caaatccgag gctgctccag 60
cctacttgaa gctgtccttc ccc 83
<210> 134
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
cagcgaagcg catgaactcc ttgatgatgg ccat 34
<210> 135
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
cagcatggcc atcatcaagg agttcatgcg cttc 34
<210> 136
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
cagcagccgt acatgaactg aggggacagg atgt 34
<210> 137
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
cagcacatcc tgtcccctca gttcatgtac ggct 34
<210> 138
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
cagcttcatc acgcgctccc acttgaagcc ctcg 34
<210> 139
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
cagccgaggg cttcaagtgg gagcgcgtga tgaa 34
<210> 140
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
cagcggtgcc gcgcagcttc accttgtaga tgaa 34
<210> 141
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
cagcttcatc tacaaggtga agctgcgcgg cacc 34
<210> 142
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
cagcttgtag gtggtcttga cctcagcgtc gta 33
<210> 143
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
cagctacgac gctgaggtca agaccaccta caa 33
<210> 144
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
tcttgtggaa aggacgaaac accggctgga gtagcccccg atttgtgggg tgattacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 145
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
cctaatggat cctagtacaa aaaaagctgt aatcacccca caaatcgggg gctactccag 60
cctacttgaa gctgtccttc ccc 83
<210> 146
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
tcttgtggaa aggacgaaac accggctgaa gaagcctccg atttgtgggg tgattacagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 147
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
cctaatggat cctagtacaa aaaaagctgt aatcacccca caaatcggag gcttcttcag 60
cctacttgaa gctgtccttc ccc 83
<210> 148
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
cagcgaagcg catgaactcc ttgatgatgg ccat 34
<210> 149
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
cagcatggcc atcatcaagg agttcatgcg cttc 34
<210> 150
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
cagcagccgt acatgaactg aggggacagg atgt 34
<210> 151
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
cagcacatcc tgtcccctca gttcatgtac ggct 34
<210> 152
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
cagcttcatc acgcgctccc acttgaagcc ctcg 34
<210> 153
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
cagccgaggg cttcaagtgg gagcgcgtga tgaa 34
<210> 154
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
cagcggtgcc gcgcagcttc accttgtaga tgaa 34
<210> 155
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
cagcttcatc tacaaggtga agctgcgcgg cacc 34
<210> 156
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
cagcttgtag gtggtcttga cctcagcgtc gta 33
<210> 157
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
cagctacgac gctgaggtca agaccaccta caa 33
<210> 158
<211> 91
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
tcttgtggaa aggacgaaac accggctgtg atggacctcg atttgtgggg tagtaacagc 60
taagccctcg gggaaggaca gcttcaagta g 91
<210> 159
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
cctaatggat cctagtacaa aaaaaagctg ttactacccc acaaatcgag gtccatcaca 60
gcctacttga agctgtcctt cccc 84
<210> 160
<211> 91
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
tcttgtggaa aggacgaaac accggctgtg atggacctcg atttgtgggg tagtaacagc 60
ttgcttcacg taggccttgg agccgtacat g 91
<210> 161
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
cctaatggat cctagtacaa aaaaaagctg ttactacccc acaaatcgag gtccatcaca 60
gccatgtacg gctccaaggc ctac 84
<210> 162
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60
atcctcgaag ttcatcacgc gctcccactt 90
<210> 163
<211> 82
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60
accaagtggg agcgcgtgat ga 82
<210> 164
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60
atgcttcacg taggccttgg agccgtacat 90
<210> 165
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60
accatgtacg gctccaaggc ctac 84
<210> 166
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60
aaagccctcg gggaaggaca gcttcaagta 90
<210> 167
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60
acctacttga agctgtcctt cccc 84
<210> 168
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60
tcctcgaagt tcatcacgcg ctcccacttg 90
<210> 169
<211> 81
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60
ccaagtggga gcgcgtgatg a 81
<210> 170
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60
tgcttcacgt aggccttgga gccgtacatg 90
<210> 171
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60
ccatgtacgg ctccaaggcc tac 83
<210> 172
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 173
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60
cctacttgaa gctgtccttc ccc 83
<210> 174
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaattcctcg 60
aagttcatca cgcgctccca cttg 84
<210> 175
<211> 75
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatcaagt 60
gggagcgcgt gatga 75
<210> 176
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaattgcttc 60
acgtaggcct tggagccgta catg 84
<210> 177
<211> 77
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatcatgt 60
acggctccaa ggcctac 77
<210> 178
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaataagccc 60
tcggggaagg acagcttcaa gtag 84
<210> 179
<211> 77
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatctact 60
tgaagctgtc cttcccc 77
<210> 180
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60
ctcctcgaag ttcatcacgc gctcccactt 90
<210> 181
<211> 82
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60
cacaagtggg agcgcgtgat ga 82
<210> 182
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60
ctgcttcacg taggccttgg agccgtacat 90
<210> 183
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60
cacatgtacg gctccaaggc ctac 84
<210> 184
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60
caagccctcg gggaaggaca gcttcaagta 90
<210> 185
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60
cactacttga agctgtcctt cccc 84
<210> 186
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60
ttcctcgaag ttcatcacgc gctcccactt 90
<210> 187
<211> 82
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60
tccaagtggg agcgcgtgat ga 82
<210> 188
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60
ttgcttcacg taggccttgg agccgtacat 90
<210> 189
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60
tccatgtacg gctccaaggc ctac 84
<210> 190
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60
taagccctcg gggaaggaca gcttcaagta 90
<210> 191
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60
tcctacttga agctgtcctt cccc 84
<210> 192
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggcctcctcg 60
aagttcatca cgcgctccca cttg 84
<210> 193
<211> 75
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagaccaagt 60
gggagcgcgt gatga 75
<210> 194
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggcctgcttc 60
acgtaggcct tggagccgta catg 84
<210> 195
<211> 77
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagaccatgt 60
acggctccaa ggcctac 77
<210> 196
<211> 84
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggccaagccc 60
tcggggaagg acagcttcaa gtag 84
<210> 197
<211> 77
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagacctact 60
tgaagctgtc cttcccc 77
<210> 198
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60
tcctcgaagt tcatcacgcg ctcccacttg 90
<210> 199
<211> 81
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60
ccaagtggga gcgcgtgatg a 81
<210> 200
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60
tgcttcacgt aggccttgga gccgtacatg 90
<210> 201
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60
ccatgtacgg ctccaaggcc tac 83
<210> 202
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60
aagccctcgg ggaaggacag cttcaagtag 90
<210> 203
<211> 83
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60
cctacttgaa gctgtccttc ccc 83
<210> 204
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
cagcactctc tctgggttga ggttgctgac cagc 34
<210> 205
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
cagcgctggt cagcaacctc aacccagaga gagt 34
<210> 206
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
cagcaggctt tggggtgtga ctctctctgg gttg 34
<210> 207
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
cagccaaccc agagagagtc acaccccaaa gcct 34
<210> 208
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
cagccagctg cacgttctgg tgcttcgag 29
<210> 209
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
cagcctcgaa gcaccagaac gtgcagctg 29
<210> 210
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
cagccactga tcacatttaa atggcttctc acctg 35
<210> 211
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
cagccaggtg agaagccatt taaatgtgat cagtg 35
<210> 212
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
cagcaggcca cataactgca ctgatcacat tt 32
<210> 213
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
cagcaaatgt gatcagtgca gttatgtggc ct 32
<210> 214
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
cagcgttact tcatgttgat tagaggccac at 32
<210> 215
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
cagcatgtgg cctctaatca acatgaagta ac 32
<210> 216
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
acagccaaat gctggtaaca ctgtggtcca caagg 35
<210> 217
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
cagcccttgt ggaccacagt gttaccagca tttgg 35
<210> 218
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
acagcgagag cagcagcaaa ctcctttgct ccctc 35
<210> 219
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
cagcgaggga gcaaaggagt ttgctgctgc tctcg 35
<210> 220
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
acagctttgc tccctccaaa tgctggtaac actgt 35
<210> 221
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
cagcacagtg ttaccagcat ttggagggag caaag 35
<210> 222
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
acagcctgta ctggtggatg tcctcaaaag acttg 35
<210> 223
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
cagccaagtc ttttgaggac atccaccagt acagg 35
<210> 224
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
acagctccga gtccttcacc cgtttgatct gctcc 35
<210> 225
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
cagcggagca gatcaaacgg gtgaaggact cggag 35
<210> 226
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
acagctgatc tgctccctgt actggtggat gtcct 35
<210> 227
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
cagcaggaca tccaccagta cagggagcag atcag 35
<210> 228
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
acagcgtttc tggcagttct cctctcctgc acccc 35
<210> 229
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
cagcggggtg caggagagga gaactgccag aaacg 35
<210> 230
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
acagcagcac tgctgggcac agatgatttt ggtca 35
<210> 231
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
cagctgacca aaatcatctg tgcccagcag tgctg 35
<210> 232
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
acagcgattt tggtcagttt ctggcagttc tcctc 35
<210> 233
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
cagcgaggag aactgccaga aactgaccaa aatcg 35
<210> 234
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
acagcctata atggtgaata tcttcaaatg attta 35
<210> 235
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
cagctaaatc atttgaagat attcaccatt atagg 35
<210> 236
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
acagctcaga gtccttaact cttttaattt gttct 35
<210> 237
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
cagcagaaca aattaaaaga gttaaggact ctgag 35
<210> 238
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
acagctaatt tgttctctat aatggtgaat atctt 35
<210> 239
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
cagcaagata ttcaccatta tagagaacaa attag 35
<210> 240
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
acagccttgg cctggacggc gtaagaagcc aggag 35
<210> 241
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
cagcctcctg gcttcttacg ccgtccaggc caagg 35
<210> 242
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
acagccttgt gaacactggg gtcgtagtca ccata 35
<210> 243
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
cagctatggt gactacgacc ccagtgttca caagg 35
<210> 244
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
acagctagtc accatacttg gcctggacgg cgtaa 35
<210> 245
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
cagcttacgc cgtccaggcc aagtatggtg actag 35
<210> 246
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
acagcctcat agttgtccat aagtgttttg gaagg 35
<210> 247
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
cagcccttcc aaaacactta tggacaacta tgagg 35
<210> 248
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
acagccacca gctctctgac tgtaccccca gagac 35
<210> 249
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
cagcgtctct gggggtacag tcagagagct ggtgg 35
<210> 250
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
acagcccccc agagacctca tagttgtcca taagt 35
<210> 251
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
cagcacttat ggacaactat gaggtctctg ggggg 35
<210> 252
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
acagcctgtc tggtcttggc tgaggtttca atgaa 35
<210> 253
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
cagcttcatt gaaacctcag ccaagaccag acagg 35
<210> 254
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 254
acagctacca gtgtgtaaaa agcatcttca acacc 35
<210> 255
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
cagcggtgtt gaagatgctt tttacacact ggtag 35
<210> 256
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
acagctcttc aacaccctgt ctggtcttgg ctgag 35
<210> 257
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
cagcctcagc caagaccaga cagggtgttg aagag 35
<210> 258
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
acagccatta tgagacatcc ccactgcaag gcatt 35
<210> 259
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
cagcaatgcc ttgcagtggg gatgtctcat aatgg 35
<210> 260
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
acagccggcc tgtggcatcc gcccaaacct gatgg 35
<210> 261
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
cagcccatca ggtttgggcg gatgccacag gccgg 35
<210> 262
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
acagcaaacc tgatggcatt atgagacatc cccac 35
<210> 263
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
cagcgtgggg atgtctcata atgccatcag gtttg 35
<210> 264
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
acagcctcaa tcatcctgct gtccacaggc agggt 35
<210> 265
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
cagcaccctg cctgtggaca gcaggatgat tgagg 35
<210> 266
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
acagcggctg attcgctgtg acttcgaatt gcatc 35
<210> 267
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
cagcgatgca attcgaagtc acagcgaatc agccg 35
<210> 268
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
acagccgaat tgcatcctca atcatcctgc tgtcc 35
<210> 269
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
cagcggacag caggatgatt gaggatgcaa ttcgg 35
<210> 270
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
acagcctggt gaggattcca gtcgctgtca aaaat 35
<210> 271
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
cagcattttt gacagcgact ggaatcctca ccagg 35
<210> 272
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
acagcgcggt gggctcggtc ctgcgcttgc aggtc 35
<210> 273
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
cagcgacctg caagcgcagg accgagccca ccgcg 35
<210> 274
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
acagcgcttg caggtcctgg tgaggattcc agtcg 35
<210> 275
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
cagccgactg gaatcctcac caggacctgc aagcg 35
<210> 276
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
acagcatcac aattggctcg gcccccattc ccaca 35
<210> 277
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
cagctgtggg aatgggggcc gagccaattg tgatg 35
<210> 278
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
acagcgtgca gctcctcagt cacaatcagg gaagc 35
<210> 279
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
cagcgcttcc ctgattgtga ctgaggagct gcacg 35
<210> 280
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
acagcatcag ggaagcatca caattggctc ggccc 35
<210> 281
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
cagcgggccg agccaattgt gatgcttccc tgatg 35
<210> 282
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
ttattccttc catcatcccg acagcagtgg 30
<210> 283
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
ccactgctgt cgggatgatg gaaggaataa 30
<210> 284
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
aagccctcgg ggaaggacag cttcaagtag 30
<210> 285
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
ctacttgaag ctgtccttcc ccgagggctt 30
<210> 286
<211> 10
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
ctcccacttg 10
<210> 287
<211> 10
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
caagtgggag 10
<210> 288
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
acgcgctccc acttg 15
<210> 289
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
caagtgggag cgcgt 15
<210> 290
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
tcatcacgcg ctcccacttg 20
<210> 291
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
caagtgggag cgcgtgatga 20
<210> 292
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
gaagttcatc acgcgctccc acttg 25
<210> 293
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
caagtgggag cgcgtgatga acttc 25
<210> 294
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
tcctcgaagt tcatcacgcg ctcccacttg 30
<210> 295
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
caagtgggag cgcgtgatga acttcgagga 30
<210> 296
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
cgccgtcctc gaagttcatc acgcgctccc acttg 35
<210> 297
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
caagtgggag cgcgtgatga acttcgagga cggcg 35
<210> 298
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg 40
<210> 299
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
caagtgggag cgcgtgatga acttcgagga cggcggcgtg 40
<210> 300
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttg 45
<210> 301
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgac 45
<210> 302
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg 50
<210> 303
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 50

Claims (34)

1.鉴定新的功能性Cas13蛋白的方法，其包括：
a)CRISPR array区域的鉴定，其包括从细菌、古细菌基因组以及宏基因组序列中鉴定CRISPR array区域；
b)在所鉴定的CRISPR array区域附近搜寻Cas13候选蛋白，其包括在所鉴定区域的上游和下游检索包含目标结构域的蛋白质，所述目标结构域为HEPN结构域和其他具有RNA切割活性的结构域；
c)对检索到的蛋白质进行氨基酸(AA)长度分析，选取包含至少2个目标结构域并且长度小于950个氨基酸的Cas13候选蛋白；
d)从c)得到的结果中除去功能已知的蛋白质；以及
e)对候选蛋白进行功能验证，筛选出功能性Cas13蛋白。
2.根据权利要求1所述的方法，其中所述目标结构域为氨基酸序列RX1X2X3X4H(R4xH)、RX1X2X3X4X5H(R5xH)和RX1X2X3X4X5X6H(R6xH)，其中X1、X2、X3、X4、X5、X6为任何氨基酸，其中X1优选为N、Q、H或D，其中R4xH中的X1更优选为N、D、Q、H。
3.根据权利要求1所述的方法，其中所述HEPN结构域来自于表3中的序列。
4.根据权利要求1所述的方法，其中所述其他具有RNA切割活性的结构域来自表2中的序列。
5.根据权利要求1所述的方法，其中在步骤b)中，对所鉴定的CRISPR array区域上下游距离最近的各6个ORF进行目标结构域检索。
6.根据权利要求1所述的方法，其中所述功能验证包括验证对RNA的切割活性，所述Cas13蛋白能够与所在所属物种基因组的CRISPR array上的同向重复(DR)序列和间隔区域结合形成复合物而发挥RNA切割作用。
7.通过权利要求1所述方法筛选到的Cas13蛋白。
8.根据权利要求7所述的Cas13蛋白，其包含如SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列，或具有一个或更多个残基的保守氨基酸取代的SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列。
9.根据权利要求7或8所述Cas13蛋白，其RNA切割活性被保留。
10.根据权利要求7或8所述Cas13蛋白，其HEPN结构域或RNA切割结构域经进一步修饰或改造，而使其RNA切割活性降低或消除，成为RNA切割活性降低或消除的dCas13。
11.根据权利要求7至10中任一项所述的Cas13蛋白，其中所述Cas13蛋白与一个或更多个异源功能性结构域融合，其中所述融合在所述Cas13蛋白的N端、C端或者内部。
12.根据权利要求11所述的Cas13蛋白，其中所述一个或更多个异源功能性结构域具有以下活性：脱氨酶如胞苷脱氨基酶和脱氧腺苷脱氨基酶、甲基化酶、去甲基化酶、转录激活、转录抑制、核酸酶、单链RNA裂解、双链RNA裂解、单链DNA裂解、双链DNA裂解、DNA或RNA连接酶、报告蛋白、检测蛋白、定位信号、或其任意组合。
13.核酸分子，其包含编码权利要求7至12中任一项所述Cas13蛋白的核苷酸序列。
14.根据权利要求13所述的核酸分子，其针对在特定宿主细胞中的表达而进行了密码子优化。
15.根据权利要求14所述的核酸分子，其中所述宿主细胞是真核生物细胞，优选人细胞。
16.根据权利要求13至15中任一项所述的核酸分子，其包含与编码Cas13的核苷酸序列有效链接的启动子，其为组成型启动子、诱导型启动子、组织特异性启动子或发育特异性启动子。
17.表达载体，其包含权利要求13至16中任一项所述核酸分子。
18.根据权利要求17所述的表达载体，其为腺相关病毒(AAV)、腺病毒、慢病毒、逆转录病毒、单纯孢疹病毒、溶瘤病毒。
19.递送系统，包含(1)权利要求17所述的表达载体，或权利要求6至11中任一项所述的Cas13蛋白；以及(2)递送载体。
20.根据权利要求19所述的递送系统，其中所述递送载体是纳米颗粒、脂质体、外泌体、微囊泡或基因枪。
21.CRISPR-Cas系统，其包含：(1)根据权利要求7至12中任一项所述的Cas13蛋白或者其衍生物或功能片段，或权利要求13至16中任一项所述核酸分子；(2)用于靶向目标RNA的gRNA序列。
22.根据权利要求21所述的CRISPR-Cas系统，Cas13蛋白的功能片段应指含有一个或多个SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任何一个氨基酸(例如1、2、3、4、5、6、7、8、9或10个残基)的添加、缺失和/或取代(例如保守取代)。
23.根据权利要求21所述的CRISPR-Cas系统，Cas13蛋白的衍生物应指至少具有与SEQID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任意一个蛋白片段达到≥70％氨基酸序列同一性(如70％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％的一致性)。
24.根据权利要求21所述的CRISPR-Cas系统，其中所述gRNA序列包含同向重复(DR)序列和靶向靶RNA部分的间隔区域的序列。
25.根据权利要求24所述的CRISPR-Cas系统，其中所述DR序列为表4中所示序列；其中所述间隔区序列为15-60个核苷酸，优选25-50个核苷酸，更优选30个核苷酸。
26.根据权利要求25所述CRISPR-Cas系统，其中所述DR序列可以是对应以下任一项的衍生物，其中所述衍生物(i)与表4中所示序列中的任一个相比，具有一个或多个(例如1、2、3、4、5、6、7、8、9或10)个核苷酸的添加、缺失、或取代；(ii)与表4中所示序列中任何一个具有至少20％、30％、40％、50％、60％、70％、80％、90％、95％或97％的序列同一性；(iii)在严格条件下与表4中所示序列任意一个，或与(i)和(ii)中的任意一个杂交；或(iv)是(i)-(iii)中任何一个的互补物，条件是所述衍生物非表4中所示序列中的任何一个，并且所述衍生物编码一个RNA，或本身即是一个RNA，所述RNA与SEQ ID NO：75-105编码的任意RNA基本保持相同的二级结构。
27.根据权利要求21所述的CRISPR-Cas系统，其还包含：(3)靶RNA。
28.根据权利要求21所述的CRISPR-Cas系统，其引起靶RNA序列的降解、切割或序列的改变。
29.根据权利要求27所述的CRISPR-Cas系统，其中所述靶RNA是mRNA或ncRNA，包括选自lncRNA、miRNA、misc_RNA、Mt_rRNA、Mt_tRNA、rRNA、scaRNA、scRNA、snoRNA、snRNA、sRNA的非编码RNA。
30.细胞，其包含权利要求7至12中任一项所述Cas13蛋白、权利要求13至16中任一项所述核酸分子、权利要求17或18所述表达载体、权利要求19或20所述递送系统、或权利要求21至29中任一项所述CRISPR-Cas系统。
31.根据权利要求30所述的细胞，其为原核细胞或真核细胞，优选人细胞。
32.降解或切割目的细胞中靶RNA、修饰目的细胞中靶RNA的序列的方法，其包括使用权利要求7至12中任一项所述Cas13蛋白、权利要求13至16中任一项所述核酸分子、权利要求17或18所述表达载体、权利要求19或20所述递送系统、或权利要求21至29中任一项所述CRISPR-Cas系统。
33.根据权利要求32所述的方法，所述目的细胞为原核细胞或真核细胞，优选人细胞。
34.根据权利要求32所述的方法，其中所述目的细胞为离体细胞、体外细胞或体内细胞。
CN202210632683.7A 2021-06-04 2022-06-06 新型Cas13蛋白及其筛选方法和应用 Pending CN115678872A (zh)

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