CN115671364B - Foam bacterial cellulose dressing with gradient structure and preparation method thereof - Google Patents
Foam bacterial cellulose dressing with gradient structure and preparation method thereof Download PDFInfo
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- 229920002749 Bacterial cellulose Polymers 0.000 title claims abstract description 139
- 239000005016 bacterial cellulose Substances 0.000 title claims abstract description 139
- 239000006260 foam Substances 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000010410 layer Substances 0.000 claims abstract description 89
- 239000002344 surface layer Substances 0.000 claims abstract description 34
- 230000003014 reinforcing effect Effects 0.000 claims abstract description 29
- 239000011148 porous material Substances 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000000835 fiber Substances 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 63
- 238000000855 fermentation Methods 0.000 claims description 39
- 230000004151 fermentation Effects 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 15
- 238000004061 bleaching Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 241000589216 Komagataeibacter hansenii Species 0.000 claims description 12
- 239000007844 bleaching agent Substances 0.000 claims description 12
- 238000005187 foaming Methods 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 10
- 239000004088 foaming agent Substances 0.000 claims description 9
- 241001136169 Komagataeibacter xylinus Species 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 239000003381 stabilizer Substances 0.000 claims description 7
- 241000589220 Acetobacter Species 0.000 claims description 6
- -1 polypropylene Polymers 0.000 claims description 6
- 239000004952 Polyamide Substances 0.000 claims description 5
- 229920002647 polyamide Polymers 0.000 claims description 5
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 230000003472 neutralizing effect Effects 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000463 material Substances 0.000 abstract description 6
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 8
- 229920002678 cellulose Polymers 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 description 4
- 230000035876 healing Effects 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 239000004156 Azodicarbonamide Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229940072056 alginate Drugs 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- XOZUGNYVDXMRKW-AATRIKPKSA-N azodicarbonamide Chemical compound NC(=O)\N=N\C(N)=O XOZUGNYVDXMRKW-AATRIKPKSA-N 0.000 description 2
- 235000019399 azodicarbonamide Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 2
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 239000000230 xanthan gum Substances 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 235000010493 xanthan gum Nutrition 0.000 description 2
- 229940082509 xanthan gum Drugs 0.000 description 2
- ARIWANIATODDMH-AWEZNQCLSA-N 1-lauroyl-sn-glycerol Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)CO ARIWANIATODDMH-AWEZNQCLSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 239000004155 Chlorine dioxide Substances 0.000 description 1
- ARIWANIATODDMH-UHFFFAOYSA-N Lauric acid monoglyceride Natural products CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 244000061121 Rauvolfia serpentina Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- 206010046914 Vaginal infection Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 235000019398 chlorine dioxide Nutrition 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000004872 foam stabilizing agent Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229960004063 propylene glycol Drugs 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- UKLNMMHNWFDKNT-UHFFFAOYSA-M sodium chlorite Chemical compound [Na+].[O-]Cl=O UKLNMMHNWFDKNT-UHFFFAOYSA-M 0.000 description 1
- 229960002218 sodium chlorite Drugs 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 229940045872 sodium percarbonate Drugs 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Abstract
The invention discloses a foam bacterial cellulose dressing with a gradient structure and a preparation method thereof. The foam bacterial cellulose dressing with the gradient structure is characterized in that: comprises a bacterial cellulose surface layer, a reinforcing net layer and a bacterial cellulose inner layer; the pore diameter of the bacterial cellulose inner layer is 1-200 mu m, and the porosity is 80-95%. The foam bacterial cellulose dressing prepared by the invention has multiple functions, namely a foam bacterial fiber layer and a reinforcing layer, so that the dressing material has higher mechanical strength, on one hand, the complete form and the yield of the material can be maintained in the processing process, and on the other hand, the suture fixation of the dressing in clinical use can be facilitated.
Description
Technical Field
The invention belongs to the field of biomedical materials, and particularly relates to a foam bacterial cellulose dressing with a gradient structure and a preparation method thereof.
Background
Bacterial cellulose dressing is a popular high-end dressing product in recent years, has good soft dressing property, liquid absorption and permeability, porous air permeability, high mechanical strength, high purity of chemical components and other physical and chemical properties, has no immunogenicity and good biocompatibility, and is a well-known and ideal wound repair wet dressing.
Bacterial cellulose dressing products are widely applied to the field of repairing superficial wound surfaces such as shallow secondary, deep secondary and skin supply areas. After the bacterial cellulose dressing is applied to the wound surface, the pain can be relieved, a suitable wet healing environment is provided for wound surface healing, and meanwhile, seepage generated by the wound surface is absorbed, so that the wound area liquid is prevented from influencing the healing.
However, the liquid seepage absorbing capacity of the bacterial cellulose dressing products on the market is only suitable for medium-low liquid seepage wound surfaces, is difficult to directly use for high-liquid seepage wound surfaces like foam dressing, alginate dressing and the like, and limits the application range of bacterial cellulose to a certain extent. Increasing the pore size of bacterial cellulose is the most dominant solution.
The current method for preparing the large-aperture bacterial cellulose mainly comprises the following steps: crushing and freeze drying to form, soaking and dissolving to form pores, physical perforation to form foaming agent film, etc.
The crushing and freeze-drying forming method is to crush and scatter bacterial cellulose, make certain modification composite and freeze-dry and form the porous composite material. But the mechanical strength of the broken materials is difficult to ensure.
The soaking and dissolving pore-forming method is to soak the bacterial cellulose film in hydrogen peroxide solution for over 24 hr, add sodium chlorite to produce great amount of pores inside the bacterial cellulose gel, quench and freeze dry to form. However, the pore size in this manner is difficult to control.
The physical perforation method is to repeatedly cut the bacterial cellulose membrane to the required porosity and pore diameter by ultraviolet laser, but most of the pores are through straight pores with the diameter of 1mm-2mm, the pore diameter is overlarge, and the water locking capacity is limited.
The foaming agent film forming method is to add a foaming agent and a foam stabilizer into a bacterial cellulose film to form a foam layer on the surface of fermentation liquor, and the foam layer is used as a template for forming the foam cellulose film. But the thickness and pore size control of the foam layer is critical to the film forming effect of the foamed cellulose.
In addition, the foam bacterial cellulose prepared by the method has the problem of lower mechanical strength because the original fiber structure of the bacterial cellulose is damaged by the porous structure, and the clinical use of the foam bacterial cellulose dressing product can be influenced.
Disclosure of Invention
The invention provides a foam bacterial cellulose dressing with a gradient structure and a preparation method thereof. The dressing is prepared by adopting a synthetic biological fermentation process and has a multilayer gradient structure comprising a bacterial cellulose surface layer, a reinforcing mesh layer and a bacterial cellulose inner layer. The bacterial cellulose surface layer has a more compact pore structure, and is used as a surface layer of the dressing to play a role in bacteria resistance and ventilation. The reinforcing net layer is used as an intermediate layer of the dressing to play a role in mechanical support. The bacterial cellulose lining layer has a looser through pore structure, the average pore diameter range is 1-200 mu m, and the bacterial cellulose lining layer is used as a dressing lining layer and directly contacts with a wound surface, so that the bacterial cellulose lining layer has stronger and faster liquid seepage absorption capability. The invention has simple preparation process, high mechanical strength, strong liquid absorptivity and good clinical practical value.
The aim of the invention is achieved by the following technical scheme:
a foam bacterial cellulose dressing with a gradient structure comprises a bacterial cellulose surface layer, a reinforcing mesh layer and a bacterial cellulose inner layer; the bacterial cellulose inner layer is contacted with skin (wound surface);
the three-layer structure of the bacterial cellulose surface layer, the reinforcing net layer and the bacterial cellulose inner layer has the thickness ratio of 1: (0.1-1): (1-5).
The bacterial cellulose inner layer is obtained through foaming fermentation, has a more loose through pore structure, the pore diameter range is 1-200 mu m, and the porosity is 80-95%. As the inner layer of the dressing, the dressing is directly contacted with the wound surface, and has stronger and faster liquid seepage absorption capacity.
Preferably, the thickness of the bacterial cellulose inner layer is 0.1 mm-3.0 mm.
The bacterial cellulose surface layer is obtained through common fermentation and has a more compact pore structure; the thickness of the bacterial cellulose surface layer is 0.1-2.0 mm, the aperture is 0.5-1.5 mu m, the porosity is 40-60%, and the bacterial cellulose surface layer can serve as a surface layer of dressing to play a role in resisting bacteria and ventilation.
The reinforcing net layer is one of a polyamide fiber net, a polypropylene fiber net, a polyester fiber net and a cotton fiber net with biological safety. The reinforcing net layer is used as an intermediate layer of the dressing to play a role in mechanical support.
Preferably, the mesh size of the reinforcing mesh layer is 0.5mm to 5.0mm. The thickness of the reinforcing mesh layer is related to the balance of dressing strength and breathability, preferably 1.0mm to 2.0mm.
The preparation method of the foam bacterial cellulose dressing with the gradient structure comprises the following steps:
(1) Preparation of bacterial cellulose surface layer: sterilizing the fermentation medium at high temperature and high pressure, adding the fermentation bacteria liquid, and fermenting at the constant temperature of 30 ℃ for 5-10 days; performing post-treatment on the bacterial cellulose obtained after fermentation, then performing flat pressing to a thickness of 0.1-2.0 mm, and performing high-temperature high-pressure sterilization to obtain a bacterial cellulose surface layer;
(2) And (3) fermenting to obtain a three-layer structure dressing: adding 0.1-5.0 wt% foaming agent and 0.1-8.0 wt% foam stabilizer into a fermentation medium, sterilizing at high temperature and high pressure, and adding zymophyte bacteria liquid; foaming the culture medium by adopting a foaming instrument to form a uniform foam layer with the thickness of 5 mm-30 mm. The initial foam layers with different thicknesses can be obtained by controlling the foaming time, and the final foam layers with different thicknesses can be obtained by different types of foam stabilizers and different addition amounts thereof; tightly covering the reinforcing net layer above the foam layer, tightly covering the bacterial cellulose surface layer on the surface of the reinforcing net layer, and fermenting at a constant temperature of 30 ℃ for 5-10 days to obtain a dressing with a three-layer structure; the obtained dressing is subjected to aftertreatment to obtain a foam bacterial cellulose dressing with a gradient structure;
the inoculum size of the zymophyte bacterial liquid in the steps (1) and (2) is 1.0-5.0% of the volume ratio of the culture medium.
The bacterial density parameter OD600 absorbance value of the fermentation bacterial liquid in the steps (1) and (2) is 0.1-2.0.
The zymophyte in the steps (1) and (2) is more than one of acetobacter hansenii (Gluconacetobacter hansenii), acetobacter hansenii (Komagataeibacter hansenii), acetobacter xylosoxydans (Gluconacetobacter xylinus) or acetobacter xylosoxydans (Komagataeibacter xylinus);
the fermentation culture medium in the steps (1) and (2) contains 0.1 to 1.0 weight percent of monopotassium phosphate, 0.1 to 1.0 weight percent of dipotassium phosphate, 0.01 to 0.15 weight percent of magnesium sulfate, 0.1 to 2.0 weight percent of ammonium sulfate, 0.01 to 0.25 weight percent of citric acid, 0.5 to 10.0 weight percent of glycerol, 0.5 to 5.0 weight percent of soybean peptone, 0.5 to 5.0 weight percent of absolute ethyl alcohol and the balance of purified water;
the post-treatment in the step (1) is to rinse the bacterial cellulose with clear water and then soak the bacterial cellulose in a sodium hydroxide solution with the concentration of 1.0 to 5.0 weight percent; and (3) after washing with clear water, placing the mixture in a mixed solution of 0.1 to 5.0 weight percent of bleaching agent and 0.1 to 2.5 weight percent of sodium hydroxide solution for bleaching to a white semitransparent state.
The foaming agent in the step (2) is more than one of lecithin, xanthan gum, propylene glycol ester, triglyceryl ester, sorbitan fatty acid ester, monoglyceride, diglyceride, glycerol monolaurate, succinyl monoglyceride or azodicarbonamide.
The foam stabilizer in the step (2) is more than one of polyacrylamide, polyvinyl alcohol, dodecyl dimethyl amine oxide, alkyl alcohol amide, starch or cellulose.
The post-treatment of step (2) comprises the following steps:
taking out the foam bacterial cellulose dressing film obtained by fermentation, washing with clear water, and then soaking in 1.0wt% -5.0wt% sodium hydroxide solution; washing with clear water, and then bleaching in a mixed solution of 0.1-5.0 wt% of bleaching agent and 0.1-2.5 wt% of sodium hydroxide solution to a white semitransparent state; finally, neutralizing the dressing film to be neutral by adopting 1.0wt% -5.0wt% of acid solution to obtain the foam bacterial cellulose dressing with a gradient structure;
the bleaching agent is more than one of hydrogen peroxide, sodium hypochlorite, sodium percarbonate, chlorine dioxide or sodium sulfite;
the acidic solution is more than one of hydrochloric acid, sulfuric acid, citric acid or glacial acetic acid.
Compared with the prior art, the invention has the following advantages and effects:
1. the foam bacterial cellulose dressing prepared by the invention has multiple functions, namely a foam bacterial fiber layer and a reinforcing layer, so that the dressing material has higher mechanical strength, on one hand, the complete form and the yield of the material can be maintained in the processing process, and on the other hand, the suture fixation of the dressing in clinical use can be facilitated.
2. The foam bacterial cellulose dressing prepared by the invention has an up-down gradient structure and is similar to the natural dermis and epidermis structures of a human body. The loose porous foam layer is contacted with the wound surface of the human body, which is beneficial to smooth blood circulation; the upper surface is a reinforcing layer which is helpful for blocking bacteria and controlling water and promoting the wet healing of wound surfaces.
3. The foam bacterial cellulose dressing prepared by the invention has good liquid absorption performance, has larger liquid absorption and seepage capacity compared with the common bacterial cellulose dressing, has wider application range compared with the foam dressing or alginate dressing, and can be used for wet wound surfaces and can also be directly used for dry wound surfaces.
4. In the preparation process of the foam bacterial cellulose dressing, the surface bacterial cellulose and the reinforcing net layer are covered on the foam layer for fermentation, so that the bacterial cellulose inner layer with specific pore diameter and porosity is obtained through fermentation; in the fermentation process, the formed bacterial cellulose is also embedded into the reinforcing mesh layer and the surface bacterial cellulose, so that the dressing with the three-layer structure is more integrated, and the mechanical strength and tearing resistance of the dressing are improved.
5. In the preparation process of the foam bacterial cellulose dressing, the bleaching process adopts a mode of mixing the bleaching agent and sodium hydroxide, thereby being beneficial to accelerating the bleaching process of the cellulose dressing and effectively avoiding the problems of white spots and uneven bleaching.
Drawings
FIG. 1 is a schematic structural view of a gradient structured foam bacterial cellulose dressing of the present invention.
Fig. 2 shows the cross-sectional microstructure of the gradient structured foam bacterial cellulose dressing obtained in example 1.
Fig. 3 shows the surface microstructure of the gradient structured foam bacterial cellulose dressing obtained in example 1.
Fig. 4 shows the inner microstructure (large pore size) of the gradient structured foam bacterial cellulose dressing obtained in example 1.
Fig. 5 shows the inner microstructure (small pore size) of the gradient structured foam bacterial cellulose dressing obtained in example 2.
Fig. 6 is the inner layer microstructure (mesopore size) of the gradient structure foam bacterial cellulose dressing obtained in example 3.
Fig. 7 is a wet break force comparison of gradient structure foam detail cellulose dressing obtained in examples 1-3 with other bacterial cellulose dressing.
Fig. 8 is a liquid absorbency comparison of the gradient structure foam detail cellulose dressing obtained in examples 1-3 with other bacterial cellulose dressings.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but embodiments of the present invention are not limited thereto.
Example 1
The preparation method of the foam bacterial cellulose dressing with the gradient structure comprises the following steps:
(1) Preparation of bacterial cellulose surface layer: sterilizing the fermentation medium at high temperature and high pressure, adding Acetobacter hansenii (Gluconacetobacter hansenii) fermentation broth accounting for 1% of the volume of the medium, fermenting at constant temperature of 30 ℃ for 5 days, wherein the OD600 value of the broth is 1.2; washing the bacterial cellulose obtained after fermentation by clear water, and soaking the bacterial cellulose in a sodium hydroxide solution with the weight percent of 2 percent; washing with clear water, and bleaching in a mixed solution of 1.2wt% of bleaching agent and 0.5wt% of sodium hydroxide solution to a white semitransparent state; and flattening the bacterial cellulose to a thickness of 1mm, and sterilizing at high temperature and high pressure to obtain the bacterial cellulose surface layer.
(2) Fermentation: 2.5 weight percent of xanthan gum foaming agent and 1.5 weight percent of polyacrylamide foam stabilizer are added into a fermentation medium, after high-temperature high-pressure sterilization, acetobacter hansenii (Gluconacetobacter hansenii) zymophyte liquid accounting for 1 percent of the volume of the medium is added, and the OD600 value of the liquid is 1.2. Foaming the culture medium by adopting a foaming instrument to form a uniform foam layer with the thickness of 10 mm; tightly covering a polyamide fiber reinforced net layer with the mesh size of 0.5mm above the foam layer, and tightly covering a bacterial cellulose surface layer on the surface of the reinforced net layer; fermenting at 30deg.C for 5 days; the obtained dressing is washed by clean water and is soaked in a sodium hydroxide solution with the concentration of 2 weight percent; washing with clear water, and then placing in a mixed solution of 1.2wt% of bleaching agent and 0.5wt% of sodium hydroxide solution for bleaching to a white semitransparent state to obtain a foam bacterial cellulose dressing with a gradient structure;
the fermentation medium in the steps (1) and (2) contains 0.5wt% of monopotassium phosphate, 0.5wt% of dipotassium phosphate, 0.05wt% of magnesium sulfate, 0.5wt% of ammonium sulfate, 0.05wt% of citric acid, 2.0wt% of glycerol, 1.5wt% of soybean peptone, 1.5wt% of absolute ethanol and the balance of purified water;
according to the above process, a foam bacterial cellulose dressing with a gradient structure is obtained, the schematic diagram of which is shown in table 1. The cross section of the dressing obtained in example 1, the microstructure of the top and inner layers are shown in figures 2, 3 and 4, respectively; the wet break force and liquid absorbency of the resulting dressing are shown in fig. 7 and 8, respectively.
The thickness ratio of the bacterial cellulose surface layer to the bacterial cellulose reinforcing net layer to the bacterial cellulose inner layer is 1:0.1:3. Wherein the pore diameter of the inner layer of the bacterial cellulose ranges from 90 mu m to 100 mu m, the porosity is 90%, and the thickness is 3mm; the thickness of the reinforcing mesh layer is 1.0mm; the pore diameter of the bacterial cellulose surface layer ranges from 0.8 mu m to 1.0 mu m, the porosity is 45%, and the thickness is 1mm. The detection shows that the wet state breaking force of the foam bacterial cellulose dressing with the gradient structure is 6.7N, and the liquid absorption performance is 205%.
Example 2
The preparation method of the foam bacterial cellulose dressing with the gradient structure comprises the following steps:
(1) Preparation of bacterial cellulose surface layer: sterilizing the fermentation medium at high temperature and high pressure, adding a fermentation fungus liquid of colpitis bacillus (Komagataeibacter xylinus) accounting for 1.5% of the volume of the medium, and fermenting at constant temperature of 30 ℃ for 5 days, wherein the OD600 value of the fungus liquid is 0.8; washing the bacterial cellulose obtained after fermentation by clear water, and soaking the bacterial cellulose in a sodium hydroxide solution with the weight percent of 3.0 percent; washing with clear water, and bleaching in a mixed solution of 2.0wt% of bleaching agent and 1.0wt% of sodium hydroxide solution to a white semitransparent state; and flattening the bacterial cellulose to a thickness of 0.5mm, and sterilizing at high temperature and high pressure to obtain the bacterial cellulose surface layer.
(2) Fermentation: adding 0.5wt% of monoglyceride foaming agent and 0.1wt% of starch foam stabilizer into a fermentation medium, sterilizing at high temperature and high pressure, and adding fermentation bacteria liquid of Rauwolfia (Komagataeibacter xylinus) accounting for 1.5% of the volume of the medium, wherein the OD600 value of the bacteria liquid is 0.8; foaming the culture medium by adopting a foaming instrument to form a uniform foam layer with the thickness of 8 mm; tightly covering a polyamide fiber reinforced net layer with a mesh size of 1.0mm above the foam layer, and tightly covering a bacterial cellulose surface layer on the surface of the reinforced net layer; fermenting at 30deg.C for 5 days; the obtained dressing is washed by clean water and is soaked in 3.0 weight percent sodium hydroxide solution; washing with clear water, and then placing in a mixed solution of 2.0wt% of bleaching agent and 1.0wt% of sodium hydroxide solution for bleaching to a white semitransparent state to obtain a foam bacterial cellulose dressing with a gradient structure;
the fermentation medium in the steps (1) and (2) contains 1.0wt% of monopotassium phosphate, 1.0wt% of dipotassium phosphate, 0.15wt% of magnesium sulfate, 1.5wt% of ammonium sulfate, 0.25wt% of citric acid, 10.0wt% of glycerol, 3.5wt% of soybean peptone, 5.0wt% of absolute ethyl alcohol and the balance of purified water;
according to the process, the foam bacterial cellulose dressing with a gradient structure is obtained, and the inner microstructure of the obtained dressing is shown in figure 5; the wet break force and liquid absorbency of the resulting dressing are shown in fig. 7 and 8, respectively.
The thickness ratio of the bacterial cellulose surface layer to the bacterial cellulose reinforcing net layer to the bacterial cellulose inner layer is 1:0.2:2. Wherein the pore diameter of the inner layer of the bacterial cellulose is 5-10 mu m, the porosity is 85%, and the thickness is 2mm; the thickness of the reinforcing mesh layer is 0.6mm; the pore diameter of the bacterial cellulose surface layer ranges from 0.9 mu m to 1.0 mu m, the porosity is 40%, and the thickness is 1mm. The detection shows that the wet breaking force of the foam bacterial cellulose dressing with the gradient structure is 6.9N, and the liquid absorption performance is 187%.
Example 3
The preparation method of the foam bacterial cellulose dressing with the gradient structure comprises the following steps:
(1) Preparation of bacterial cellulose surface layer: sterilizing the fermentation medium at high temperature and high pressure, adding Acetobacter xylosojae (Gluconacetobacter xylinus) fermentation bacteria liquid accounting for 5.0% of the volume of the medium, fermenting at constant temperature of 30 ℃ for 5 days, wherein the OD600 value of the bacteria liquid is 2.0; washing the bacterial cellulose obtained after fermentation by clear water, and soaking the bacterial cellulose in a 4.0wt% sodium hydroxide solution; washing with clear water, and bleaching in a mixed solution of 4.0wt% of bleaching agent and 2.0wt% of sodium hydroxide solution to a white semitransparent state; and flattening the bacterial cellulose to a thickness of 1.5mm, and sterilizing at high temperature and high pressure to obtain the bacterial cellulose surface layer.
(2) Fermentation: 2.5 weight percent of azodicarbonamide foaming agent and 2.0 weight percent of dodecyl dimethyl amine oxide foam stabilizer are added into a fermentation medium, after high-temperature high-pressure sterilization, acetobacter xylosojae (Gluconacetobacter xylinus) zymophyte liquid accounting for 5.0 percent of the volume of the medium is added, and the OD600 value of the liquid is 2.0; foaming the culture medium by adopting a foaming instrument to form a uniform foam layer with the thickness of 20 mm; tightly covering a polyamide fiber reinforced net layer with a mesh size of 2.0mm above the foam layer, and tightly covering a bacterial cellulose surface layer on the surface of the reinforced net layer; fermenting at 30deg.C for 7 days; the obtained dressing is washed by clean water and is soaked in 4.0wt% sodium hydroxide solution; washing with clear water, and then bleaching in a mixed solution of 5.0wt% of bleaching agent and 3.0wt% of sodium hydroxide solution to obtain a foam bacterial cellulose dressing with a gradient structure;
the fermentation medium in the steps (1) and (2) contains 0.8wt% of monopotassium phosphate, 0.5wt% of dipotassium phosphate, 0.1wt% of magnesium sulfate, 1.0wt% of ammonium sulfate, 0.2wt% of citric acid, 7.5wt% of glycerol, 2.5wt% of soybean peptone, 3.0wt% of absolute ethanol and the balance of purified water;
according to the process, the foam bacterial cellulose dressing with a gradient structure is obtained, and the inner microstructure of the obtained dressing is shown in figure 6; the wet break force and liquid absorbency of the resulting dressing are shown in fig. 7 and 8, respectively.
The thickness ratio of the bacterial cellulose surface layer to the reinforcing net layer to the bacterial cellulose inner layer is 1:0.5:4. Wherein the pore diameter of the bacterial cellulose inner layer is 50-60 mu m, the porosity is 92%, and the thickness is 3.0mm; the thickness of the reinforcing mesh layer is 1.0mm; the pore diameter of the bacterial cellulose surface layer ranges from 1.0 mu m to 1.1 mu m, the porosity is 50%, and the thickness is 0.9mm. The detection shows that the wet state breaking force of the foam bacterial cellulose dressing with the gradient structure is 6.5N, and the liquid absorption performance is 228%.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (11)
1. The preparation method of the foam bacterial cellulose dressing with the gradient structure is characterized by comprising the following steps of:
(1) Preparation of bacterial cellulose surface layer: sterilizing the fermentation medium at high temperature and high pressure, adding the fermentation bacteria liquid, and fermenting at the constant temperature of 30 ℃ for 5-10 days; performing post-treatment on the bacterial cellulose obtained after fermentation, then performing flat pressing to a thickness of 0.1-2.0 mm, and performing high-temperature high-pressure sterilization to obtain a bacterial cellulose surface layer;
(2) And (3) fermenting to obtain a three-layer structure dressing: adding 0.1-5.0 wt% foaming agent and 0.1-8.0 wt% foam stabilizer into a fermentation medium, sterilizing at high temperature and high pressure, and adding zymophyte bacteria liquid; foaming the culture medium by adopting a foaming instrument to form a uniform foam layer with the thickness of 5 mm-30 mm; tightly covering the reinforcing net layer above the foam layer, tightly covering the bacterial cellulose surface layer on the surface of the reinforcing net layer, and fermenting at a constant temperature of 30 ℃ for 5-10 days to obtain a dressing with a three-layer structure; and (3) carrying out aftertreatment on the obtained dressing to obtain the foam bacterial cellulose dressing with the gradient structure.
2. The method of manufacturing according to claim 1, characterized in that: the fermentation bacteria in the step (1) and the step (2) are more than one of acetobacter hansenii (Gluconacetobacter hansenii), acetobacter hansenii (Komagataeibacter hansenii), acetobacter xylosoxydans (Gluconacetobacter xylinus) or acetobacter xylosoxydans (Komagataeibacter xylinus).
3. The method of manufacturing according to claim 1, characterized in that:
the post-treatment in the step (1) is to rinse the bacterial cellulose with clear water and then soak the bacterial cellulose in a sodium hydroxide solution with the concentration of 1.0 to 5.0 weight percent; washing with clear water, and then bleaching in a mixed solution of 0.1-5.0 wt% of bleaching agent and 0.1-2.5 wt% of sodium hydroxide solution to a white semitransparent state;
the post-treatment of step (2) comprises the following steps:
taking out the foam bacterial cellulose dressing film obtained by fermentation, washing with clear water, and then soaking in 1.0wt% -5.0wt% sodium hydroxide solution; washing with clear water, and then bleaching in a mixed solution of 0.1-5.0 wt% of bleaching agent and 0.1-2.5 wt% of sodium hydroxide solution to a white semitransparent state; and finally, neutralizing the dressing film to be neutral by adopting 1.0wt% -5.0wt% of acid solution to obtain the foam bacterial cellulose dressing with the gradient structure.
4. A gradient structured foam bacterial cellulose dressing, characterized in that: is obtainable by the process of any one of claims 1 to 3.
5. The foamed bacterial cellulose dressing according to claim 4, wherein: comprises a bacterial cellulose surface layer, a reinforcing net layer and a bacterial cellulose inner layer;
the pore diameter of the bacterial cellulose inner layer is 1-200 mu m, and the porosity is 80-95%.
6. The foamed bacterial cellulose dressing according to claim 5, wherein: the thickness of the bacterial cellulose inner layer is 0.1 mm-3.0 mm.
7. The foamed bacterial cellulose dressing according to claim 5, wherein: the three-layer structure of the bacterial cellulose surface layer, the reinforcing net layer and the bacterial cellulose inner layer has the thickness ratio of 1: (0.1-1): (1-5).
8. The foamed bacterial cellulose dressing according to claim 4, wherein: the thickness of the bacterial cellulose surface layer is 0.1-2.0 mm, the pore diameter is 0.5-1.5 mu m, and the porosity is 40-60%.
9. The foamed bacterial cellulose dressing according to claim 4, wherein: the reinforcing net layer is one of a polyamide fiber net, a polypropylene fiber net, a polyester fiber net and a cotton fiber net.
10. The foamed bacterial cellulose dressing according to claim 4, wherein: the mesh size of the reinforcing mesh layer is 0.5 mm-5.0 mm.
11. The foamed bacterial cellulose dressing according to claim 4, wherein: the thickness of the reinforcing mesh layer is 1.0 mm-2.0 mm.
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| CN103083112A (en) * | 2012-12-26 | 2013-05-08 | 东华大学 | Bacterial cellulose skin regeneration material with density structure and preparation method thereof |
| CN103233050A (en) * | 2013-04-22 | 2013-08-07 | 东华大学 | Bacterial cellulose membrane with gradient structure and preparation method thereof |
| CN103480028A (en) * | 2012-06-12 | 2014-01-01 | 钟春燕 | Bacterial cellulose composite dressing |
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| WO2022178698A1 (en) * | 2021-02-24 | 2022-09-01 | 海南光宇生物科技有限公司 | Bacterial cellulose-based porous foam dressing, preparation method therefor and use thereof |
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| CN103480028A (en) * | 2012-06-12 | 2014-01-01 | 钟春燕 | Bacterial cellulose composite dressing |
| CN102973985A (en) * | 2012-12-26 | 2013-03-20 | 东华大学 | Porous bacterial cellulose skin repair material with density structure and preparation method thereof |
| CN103083112A (en) * | 2012-12-26 | 2013-05-08 | 东华大学 | Bacterial cellulose skin regeneration material with density structure and preparation method thereof |
| CN103233050A (en) * | 2013-04-22 | 2013-08-07 | 东华大学 | Bacterial cellulose membrane with gradient structure and preparation method thereof |
| CN106191165A (en) * | 2016-08-05 | 2016-12-07 | 山东纳美德生物科技有限公司 | A kind of Bacterial cellulose bubble fermentation method |
| WO2022178698A1 (en) * | 2021-02-24 | 2022-09-01 | 海南光宇生物科技有限公司 | Bacterial cellulose-based porous foam dressing, preparation method therefor and use thereof |
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