CN115669944A - Application of donkey-hide gelatin oligopeptide in brightening skin - Google Patents
Application of donkey-hide gelatin oligopeptide in brightening skin Download PDFInfo
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- CN115669944A CN115669944A CN202211100853.3A CN202211100853A CN115669944A CN 115669944 A CN115669944 A CN 115669944A CN 202211100853 A CN202211100853 A CN 202211100853A CN 115669944 A CN115669944 A CN 115669944A
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Abstract
The invention belongs to the technical field of oral beauty foods, and particularly relates to application of donkey-hide gelatin oligopeptide in brightening skin. The invention provides application of donkey-hide gelatin oligopeptide in brightening skin. The invention proves the efficacy of the donkey-hide gelatin oligopeptide on improving the skin brightness and the white degree through a scientific experimental method, and provides a new way for nursing the facial skin.
Description
Technical Field
The invention belongs to the technical field of oral beauty foods, and particularly relates to application of donkey-hide gelatin oligopeptide in brightening skin.
Background
Skin care products such as skin cream, lotion and the like have the effects of cleaning, protecting, nourishing, beautifying and modifying facial skin, and people pay more and more attention to the care of the facial skin along with the improvement of living standard, but the external skin care products only can act on the facial skin and cannot solve the skin problems from the aspects of improving metabolism and immunity, enhancing body functions, improving skin cell activity and the like.
Donkey-hide gelatin is sweet and neutral in nature, has the effects of enriching blood, nourishing yin, moistening dryness and stopping bleeding, and is commonly used for blood deficiency and chlorosis, dizziness and palpitation, muscle flaccidity and weakness, vexation and insomnia, internal stirring of deficient wind, lung dryness and cough, overstrain and chatting blood, hematemesis and hematuria, hematochezia and metrorrhagia, gestational fetal leakage and the like. Donkey-hide gelatin is listed as the "top grade" in the famous medical literature "Shen nong Ben Cao Jing early in the east Han period, and records" tonify qi and lighten the body after long-term administration ". Modern pharmacological research also shows that the donkey-hide gelatin has the effects of enriching the blood, improving the immunity, resisting oxidation and the like. Researches find that the small-molecular donkey-hide gelatin oligopeptide (namely the donkey-hide gelatin oligopeptide) obtained by enzymolysis of donkey-hide gelatin can greatly improve the bioavailability of products, and provides a good solution for the exertion of the efficacy of the donkey-hide gelatin oligopeptide.
However, it is unknown whether the donkey-hide gelatin oligopeptide has an effective conditioning and improving effect on facial skin.
Disclosure of Invention
The invention aims to provide application of donkey-hide gelatin oligopeptide in brightening skin color, provides application of the donkey-hide gelatin oligopeptide in brightening skin color, and provides a new way for nursing facial skin.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides application of donkey-hide gelatin oligopeptide in brightening skin.
Preferably, the donkey-hide gelatin oligopeptide is micromolecule donkey-hide gelatin with the water content of 2-15 wt%, the total sugar content of 2-9 wt% and the particle size of 10-30 meshes.
Preferably, the donkey-hide gelatin oligopeptide is not added with any auxiliary materials.
Preferably, the donkey-hide gelatin oligopeptide is micromolecule donkey-hide gelatin with the water content of 5-9 wt%, the total sugar content of 3-8 wt% and the particle size of 15-25 meshes.
Preferably, the preparation method of the donkey-hide gelatin oligopeptide comprises the following steps:
(1) Adding water into the oil-free and sugar-free donkey-hide gelatin blocks, heating to melt the gelatin blocks until the gelatin blocks are dissolved into uniform gelatin solution, and sieving with a 120-mesh sieve to remove impurities to obtain first gelatin solution, wherein the mass ratio of the donkey-hide gelatin blocks to the water is 1;
(2) Cooling the first glue solution prepared in the step (1) to 28-50 ℃, adding compound protease to carry out hydrolysis reaction for 2-3 h to obtain donkey-hide gelatin hydrolysate;
(3) Heating and boiling the donkey-hide gelatin hydrolysate prepared in the step (2), maintaining the boiling state for 20-30 min to inactivate the compound protease, and performing fine filtration to obtain donkey-hide gelatin oligopeptide liquid;
(4) Spray drying the donkey-hide gelatin oligopeptide liquid prepared in the step (3) to obtain donkey-hide gelatin oligopeptide fine powder;
(5) Pressing the donkey-hide gelatin oligopeptide fine powder into large tablets, and then crushing the large tablets to prepare the donkey-hide gelatin oligopeptide with the required particle size.
The invention provides application of donkey-hide gelatin oligopeptide in brightening skin. The invention proves the efficacy of the donkey-hide gelatin oligopeptide on improving the brightness and the whiteness of skin by a scientific experimental method, and provides a new way for nursing facial skin.
Detailed Description
The invention provides application of donkey-hide gelatin oligopeptide in brightening skin.
In the invention, the donkey-hide gelatin oligopeptide is preferably micromolecule donkey-hide gelatin with the water content of 2-15 wt%, the total sugar content of 2-9 wt% and the particle size of 10-30 meshes.
In the present invention, the donkey-hide gelatin oligopeptide is preferably not added with any auxiliary materials.
In the invention, the donkey-hide gelatin oligopeptide is more preferably micromolecular donkey-hide gelatin with the water content of 5-9 wt%, the total sugar content of 3-8 wt% and the particle size of 15-25 meshes.
In the present invention, the preparation method of the donkey-hide gelatin oligopeptide is preferably as follows:
(1) Adding water into the oil-free and sugar-free donkey-hide gelatin blocks, heating to melt the gelatin blocks until the gelatin blocks are dissolved into uniform gelatin solution, and sieving with a 120-mesh sieve to remove impurities to obtain first gelatin solution, wherein the mass ratio of the donkey-hide gelatin blocks to the water is 1;
(2) Cooling the first glue solution prepared in the step (1) to 28-50 ℃, adding compound protease to carry out hydrolysis reaction for 2-3 h to obtain donkey-hide gelatin hydrolysate;
(3) Heating and boiling the donkey-hide gelatin hydrolysate prepared in the step (2), maintaining the boiling state for 20-30 min, inactivating the compound protease, and performing fine filtration to obtain donkey-hide gelatin oligopeptide liquid;
(4) Spray drying the donkey-hide gelatin oligopeptide liquid prepared in the step (3) to obtain donkey-hide gelatin oligopeptide fine powder;
(5) Pressing the donkey-hide gelatin oligopeptide fine powder into large tablets, and then crushing the large tablets to prepare the donkey-hide gelatin oligopeptide with the required particle size.
In the invention, the compound protease is one or more of papain, bromelain, neutral protease, alkaline protease and collagen hydrolase.
In the invention, the peptide segment with relative molecular mass distribution between 200 and 3000Da in the small-molecular donkey-hide gelatin fine powder accounts for more than 90 percent of the molecular weight distribution of the small-molecular donkey-hide gelatin fine powder, wherein the peptide segment distributed between 200 and 1000Da accounts for more than 80 percent of the molecular weight distribution of the small-molecular donkey-hide gelatin fine powder.
In the invention, under the conditions that the temperature is 18-25 ℃ and the humidity is 30-5%, the micromolecule donkey-hide gelatin is directly tabletted, during the tabletting process, the pressure of 10-40 kN is used for prepressing, and then the pressure of 20-80 kN is used for carrying out main pressing, thus obtaining the micromolecule donkey-hide gelatin pure powder tablet.
In the invention, the pre-pressing pressure is 15-35 kN, and the main pressure is 30-70 kN.
In the invention, the environmental temperature is controlled to be 20-23 ℃ and the humidity is controlled to be 35-50% in the tabletting process.
The invention preferably further comprises the steps of spraying a lubricant on the inner surface of a tabletting mold before tabletting, then putting the micromolecule donkey-hide gelatin into the tabletting mold with the lubricant sprayed on the inner surface for tabletting, forming to obtain a primary tabletting, and removing the lubricant adhered on the surface of the primary tabletting by adopting a vacuum dust collection mode to obtain the required micromolecule donkey-hide gelatin pure powder tablet.
In the invention, the lubricant is preferably magnesium stearate powder or superfine silica powder.
In the invention, the small-molecule donkey-hide gelatin pure powder tablet is preferably prepared by the method provided by embodiments 1 to 12 of chinese patent CN 201410301513.6.
In the application of the technical scheme, the donkey-hide gelatin oligopeptide is preferably donkey-hide gelatin oligopeptide produced by Dong' A donkey-hide gelatin Kongsuo effective company.
In the invention, the donkey-hide gelatin oligopeptide is preferably a vacuum plastic packaging bag packaged product.
In the present invention, the donkey-hide gelatin oligopeptide is preferably orally administered.
In the present invention, the donkey-hide gelatin oligopeptide is preferably orally administered, and the dosage of the oral preparation per day is preferably 2g.
In order to further illustrate the present invention, the following technical solutions provided by the present invention are described in detail with reference to the examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The performance test of facial skin improvement is carried out on the small-molecule donkey-hide gelatin (donkey-hide gelatin oligopeptide) prepared in example 1 of Chinese patent CN 201410301513.6.
The donkey-hide gelatin oligopeptide is produced by Dong' a donkey-hide gelatin GmbH.
The specific test process is as follows:
the test period of skin lightening was 12 weeks, and a total of 180 subjects were enrolled in the test and randomized into 4 groups (45 subjects/group), of which three groups of volunteers took oral donkey-hide gelatin oligopeptide and the fourth group of volunteers did not take any product as a control. Skin lightening color test was performed on one side of the cheek at random for all volunteers before (initial value, 0 weeks), 4 weeks, 8 weeks and 12 weeks after the product was taken. During the test period, 3 subjects of the group of donkey-hide gelatin oligopeptides and 3 subjects of the blank control were withdrawn for personal reasons. The data of 42 subjects in the final E-jiao oligopeptide group and the blank control group are included in the statistical result.
The specific test information is as follows:
sample name: e, donkey-hide gelatin oligopeptide;
the test purpose is as follows: testing the change condition of the characteristic value of the brightness of the facial skin after the donkey-hide gelatin oligopeptide is taken in different periods;
the number of participants: e, donkey-hide gelatin oligopeptide: 42 persons; blank control: 42 persons; sex: a woman;
age: 25-35 years old;
test area: one side cheek at random;
testing time points: before taking the product (initial value, 0 week), after taking the product for 4 weeks, after taking the product for 8 weeks, and after taking the product for 12 weeks, respectively performing tests;
the test method comprises the following steps: skin color was measured using a color difference meter. The instrument uses the chromaticity system (Lab chromaticity system) specified by the International Commission on illumination (CIE) to indicate changes in skin color. Wherein: l is brightness, the ITA ° value is skin individual type angle, the larger the value of skin color parameter L is, the larger the value of ITA ° (individual type angle) is, the brighter the skin color is, and conversely, the darker the skin is.
And (3) testing results:
(1) Skin lightening test results
Skin color test was performed on one of the randomized cheeks before, 4 weeks, 8 weeks, and 12 weeks after the test product was taken, and the results are shown in tables 1 and 2. The larger the value of the skin color parameter L, the larger the value of ITA ° (individual type angle) is, the whiter the skin color is.
TABLE 1 statistical results of skin color before, after 4, 8 and 12 weeks of product consumption
Note: comparison with initial values in table 1: * p is less than or equal to 0.05, compared with a blank control: # p.ltoreq.0.05 Table 2 ITA DEG value statistics of skin color before, after 4, 8 and 12 weeks of product consumption
Note: compared to the initial values in table 2: * p is less than or equal to 0.05, compared with a blank control： # p≤0.05
As can be seen from tables 1 and 2, the skin color parameters L x and ITA ° values in the test area of the group of donkey-hide gelatin oligopeptides were significantly increased after 4 weeks, 8 weeks and 12 weeks of administration of the test products compared to the initial values. And the difference between the L x value and the ITA ° value of the skin color parameter and the initial value after 4, 8 and 12 weeks of administration of the test product showed significant differences compared to the blank group.
The result shows that oral donkey-hide gelatin oligopeptide can obviously improve the value of Lx and the value of ITA DEG for representing the brightness of facial skin, thereby effectively improving the brightness of the facial skin.
Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and all of the embodiments belong to the protection scope of the present invention.
Claims (5)
1. An application of colla Corii Asini oligopeptide in brightening skin is provided.
2. The use of claim 1, wherein the donkey-hide gelatin oligopeptide is small molecular donkey-hide gelatin with water content of 2-15 wt%, total sugar content of 2-9 wt% and particle size of 10-30 meshes.
3. The use of claim 2, wherein the donkey-hide gelatin oligopeptide is free of any adjuvant.
4. The pure small-molecule donkey-hide gelatin powder tablet according to claim 2 or 3, wherein the donkey-hide gelatin oligopeptide contains 5-9 wt% of water, 3-8 wt% of total sugar and 15-25-mesh small-molecule donkey-hide gelatin.
5. The use of claim 1, wherein the preparation method of the donkey-hide gelatin oligopeptide comprises the following steps:
(1) Adding water into oil-free and sugar-free donkey-hide gelatin blocks, heating and melting the oil-free and sugar-free donkey-hide gelatin blocks until the oil-free and sugar-free donkey-hide gelatin blocks are dissolved into uniform gelatin solution, and sieving the uniform gelatin solution by a 120-mesh sieve to remove impurities to obtain first gelatin solution, wherein the mass ratio of the donkey-hide gelatin blocks to the water is 1;
(2) Cooling the first glue solution prepared in the step (1) to 28-50 ℃, adding compound protease to carry out hydrolysis reaction for 2-3 h to obtain donkey-hide gelatin hydrolysate;
(3) Heating and boiling the donkey-hide gelatin hydrolysate prepared in the step (2), maintaining the boiling state for 20-30 min to inactivate the compound protease, and performing fine filtration to obtain donkey-hide gelatin oligopeptide liquid;
(4) Spray drying the donkey-hide gelatin oligopeptide liquid prepared in the step (3) to obtain donkey-hide gelatin oligopeptide fine powder;
(5) Pressing the donkey-hide gelatin oligopeptide fine powder into large tablets, and then crushing the large tablets to prepare the donkey-hide gelatin oligopeptide with the required particle size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211100853.3A CN115669944A (en) | 2022-09-09 | 2022-09-09 | Application of donkey-hide gelatin oligopeptide in brightening skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211100853.3A CN115669944A (en) | 2022-09-09 | 2022-09-09 | Application of donkey-hide gelatin oligopeptide in brightening skin |
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|---|---|
| CN115669944A true CN115669944A (en) | 2023-02-03 |
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| CN202211100853.3A Pending CN115669944A (en) | 2022-09-09 | 2022-09-09 | Application of donkey-hide gelatin oligopeptide in brightening skin |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692908A (en) * | 2009-10-19 | 2010-04-14 | 浙江省医学科学院 | Double-glue pollen food with functions of enriching blood, beautifying face and nourishing skin and preparation method thereof |
| CN104107192A (en) * | 2014-06-27 | 2014-10-22 | 山东东阿阿胶股份有限公司 | Small molecular donkey-hide gelatin pure powder tablet and preparation method thereof |
| WO2021003926A1 (en) * | 2019-07-10 | 2021-01-14 | 南京中医药大学 | Donkey-derived characteristic peptide of donkey-hide gelatin and products thereof, and method for identifying donkey-hide gelatin and products thereof |
-
2022
- 2022-09-09 CN CN202211100853.3A patent/CN115669944A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692908A (en) * | 2009-10-19 | 2010-04-14 | 浙江省医学科学院 | Double-glue pollen food with functions of enriching blood, beautifying face and nourishing skin and preparation method thereof |
| CN104107192A (en) * | 2014-06-27 | 2014-10-22 | 山东东阿阿胶股份有限公司 | Small molecular donkey-hide gelatin pure powder tablet and preparation method thereof |
| WO2021003926A1 (en) * | 2019-07-10 | 2021-01-14 | 南京中医药大学 | Donkey-derived characteristic peptide of donkey-hide gelatin and products thereof, and method for identifying donkey-hide gelatin and products thereof |
Non-Patent Citations (3)
| Title |
|---|
| JUNG-SOO KIM: "Protection effect of donkey hide gelatin hydrolysates on UVB-induced photoaging of human skin fibroblasts", 《PROCESS BIOCHEMISTRY》, pages 118 * |
| 侯刚北;张新武;孙志伟;侯银臣;刘一春;: "方便型阿胶元粉的生物复合酶酶解工艺技术研究及开发", 中国酿造, no. 04 * |
| 吃不胖的小臭妈妈, pages 2 - 3, Retrieved from the Internet <URL:https://m.weibo.cn/status/4429922245941576> * |
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