CN115656166A - Multi-dimensional rock slice digital automatic acquisition system and acquisition method - Google Patents

Multi-dimensional rock slice digital automatic acquisition system and acquisition method Download PDF

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Publication number
CN115656166A
CN115656166A CN202211122441.XA CN202211122441A CN115656166A CN 115656166 A CN115656166 A CN 115656166A CN 202211122441 A CN202211122441 A CN 202211122441A CN 115656166 A CN115656166 A CN 115656166A
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module
image
images
sample
polarization
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Inventor
税蕾蕾
臧春艳
李洋冰
万欢
贺银军
李盼盼
史长林
杨纪磊
郝鹏
刘再振
赵伟
王岩岩
李志勇
蔡涛
郑薇
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China National Offshore Oil Corp CNOOC
CNOOC Energy Technology and Services Ltd
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China National Offshore Oil Corp CNOOC
CNOOC Energy Technology and Services Ltd
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Priority to CN202211122441.XA priority Critical patent/CN115656166A/en
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Abstract

The invention provides a digital automatic acquisition system for multidimensional rock slices, which comprises a computer, a database, an image processing module, a microscope module and an acquisition module, wherein the computer is used for acquiring images of the rock slices; the computer is connected with the image processor, the database, the microscope module and the acquisition module; the acquisition module can realize the switching of single-polarization detection and orthogonal-polarization detection and the detection of orthogonal polarized light with different angles; the imaging module shoots a high-definition polarization microscopic image of the rock slice and sends the high-definition polarization microscopic image to the computer; and the image processing module automatically splices the high-definition polarized light microscopic images. A digital automatic acquisition method for multi-dimensional rock slices comprises the following steps: collecting a single-polarization image; collecting an orthogonal polarized light image; collecting a fluorescence image; and image splicing and calling. The invention improves the working efficiency of rock slice photo collection, supports full-automatic slice sample multi-angle rotary scanning in orthogonal polarization and single polarization modes, and the scanning photographing area follows the rotation angle.

Description

Multi-dimensional rock slice digital automatic acquisition system and acquisition method
Technical Field
The invention belongs to the field of rock slice digitization, and particularly relates to a multidimensional rock slice digital automatic acquisition system and an acquisition method.
Background
The identification of the rock slice is an important experiment for researching the composition and the structure of the rock by using a polarization microscope, but the rock slice is aged and damaged after being stored for a long time, so the identification has great significance for electronization storage of a microscopic image of the rock slice. In the traditional rock slice identification process, an experimenter selects a representative area to take a picture and store, but the image acquisition of the whole slice cannot be realized, and especially for rock slices with strong heterogeneity, the storage of single polarization, orthogonal polarization and fluorescence whole slice images is more favorable for complete information retention and overall slice recognition. Moreover, the mineral under the mirror has the characteristics of polychromatism, wavy extinction and the like, and multi-angle single-polarization images and orthogonal-polarization images need to be acquired, so that the effect of simulating the features under the mirror to the maximum extent is achieved. Meanwhile, the traditional rock slice microscopic images are acquired piece by piece, manual sample changing is needed when a large number of slice images are acquired, and the acquisition efficiency is low, so that the batch automatic acquisition of slices is urgently needed.
CN203444153U discloses a polarisation microscopic image automatic acquisition analytical equipment, reforms transform traditional polarisation microscope, reforms transform the module and contains support, base plate, four rubber feet, objective table, control panel assembly body, removal slip table subassembly, camera assembly body and light source assembly body. The sheet is kept still through precise mechanical design and grating motor control, and the polaroid is controlled by a computer to rotate at any angle, so that the relative movement of the sheet and the polaroid is realized. The invention realizes the effect of simulating the observation of the thin sheet under the artificial microscope. But mineral signature under-mirror acquisition is not comprehensive. Only the information of the single-polarization and orthogonal-polarization rock images is collected, the function of collecting fluorescence images is not realized, and the sheet batch collection is not realized. The sample collection is single piece by piece, and the trade appearance cost of labor is high, and collection efficiency is low, and prior art does not realize the collection of slice sample universe microscopic image simultaneously.
CN202794020U discloses a polarisation image observation and collection device, and this method reforms transform traditional polarisation microscope, puts the observation object on the objective table, opens the computer, selects current situation collection mode and interval angle and can realize system automatic acquisition and save, realizes carrying out the many angle collection of single polarisation and the orthogonal polarisation to the measured object. But only multi-angle image acquisition of a single image is realized, and sheet batch acquisition work cannot be realized.
CN112215786A discloses a splicing optimization method for single-polarization and orthogonal-polarization rock slice images, which utilizes the moving regularity of an objective table when a microscopic slice sequence image is shot, performs independent calculation on homography matrixes of the pictures with few characteristic points in the splicing process, and then performs inspection and correction on the fusion positions of the pictures, thereby improving the problem that some pictures with few characteristic points cannot be spliced or spliced complete slices are staggered when the pictures are spliced. Meanwhile, as the single polarization diagram and the orthogonal polarization sequence diagram of the same slice are shot in the same mode, the position matrix information of the single polarization diagram is used for guiding the splicing of the orthogonal polarization diagram, thereby greatly reducing the time required by splicing the orthogonal polarization diagram. However, the method has a large calculation amount, cannot perform targeted processing aiming at the characteristics of the polarization microscopic image, and has a low processing speed.
In conclusion, the prior art realizes multi-angle image acquisition of a single image, fails to realize sheet batch acquisition work, cannot realize acquisition of sheet sample global microscopic images and cannot realize multi-angle image acquisition, and has slow splicing speed and large calculation amount during image splicing.
Disclosure of Invention
In view of the above, the present invention provides a digital automatic acquisition system and an acquisition method for multi-dimensional rock slices, which can solve the above problems.
In order to achieve the purpose, the technical scheme of the invention is realized as follows: a digital automatic acquisition system for multi-dimensional rock slices comprises a computer, a database, an image processing module, a microscope module and an acquisition module;
the computer is connected with the image processor, the database, the microscope module and the acquisition module;
the acquisition module and the microscope module work in a matched mode, the imaging module and the light source module are arranged in the microscope module, the acquisition module can convert light emitted by the light source module into linearly polarized light for polarized light microscopic detection, and can realize switching between single-polarization detection and orthogonal polarized light detection and detection of orthogonal polarized light with different angles;
the imaging module shoots a high-definition polarization microscopic image of the rock slice and sends the high-definition polarization microscopic image to the computer; the image processing module automatically splices the high-definition polarized light microscopic images in the computer to obtain spliced super-large images, and the super-large images are stored in a database.
Further, the microscope module comprises a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected with the imaging module, the fluorescence module, the light source module and the objective lens module, the imaging module, the fluorescence module, the objective lens module and the light source module are sequentially arranged from top to bottom, and meanwhile, the microscope control module controls the imaging module, the fluorescence module, the light source module and the objective lens module to work;
an illumination light source is arranged in the light source module, and light emitted by the illumination light source passes through a sample to be detected and then is transmitted to the objective lens module;
light entering from the objective lens module is transmitted to the imaging module after passing through an inner microscopic light path of the microscope so as to realize microscopic imaging;
the imaging module is internally provided with a CCD sensor and is used for shooting a high-definition microscopic image of the rock slice; the CCD sensor is provided with an imaging module interface which is used for installing an imaging module;
an eyepiece module is also arranged between the imaging module and the fluorescence module, and is provided with an eyepiece interface and a replaceable eyepiece;
a fluorescence illumination light source is arranged in the fluorescence module to emit a fluorescence excitation light source to a sample to be detected, and fluorescence emitted by the sample is transmitted to the ocular lens module and the imaging module through the objective lens;
the objective lens module is provided with an objective lens interface and a switchable objective lens to realize switching of objective lenses with different magnification factors.
Furthermore, the acquisition module comprises an acquisition controller, a polarization analyzer module, a sample stage module and a polarizer module; the acquisition controller is connected with the analyzer module, the sample stage module and the polarizer module, and simultaneously controls the movement and the work of the analyzer module, the sample stage module and the polarizer module;
the polarizer module is arranged between the light source module and the objective lens module, is provided with a polarizer and a stepping motor for driving the polarizer to rotate, and can convert light emitted by the light source module into linearly polarized light for polarized light microscopic detection;
the sample stage module is arranged between the polarizer module and the objective lens module and is used for placing a plurality of rock slices and driving the rock slices to move along an X axis and a Y axis so as to realize full-automatic sample introduction of the rock slices;
the analyzer module is arranged between the objective lens module and the imaging module, the analyzer module is provided with an analyzer and a stepping motor for driving the analyzer to translate and rotate, and the analyzer translates to realize the switching between single-polarization detection and orthogonal-polarization detection; the rotation of the analyzer is matched with the rotation of the polarizer to jointly realize the detection of orthogonal polarized lights with different angles.
Furthermore, the objective lens is in a rotary switching mode, a rotary position sensor is arranged in the objective lens, the microscope module is connected to a computer, and the computer can obtain the magnification factor of the objective lens selected by the microscope in real time; the sample platform module is arranged on a lifting slide block of the microscope, and the lifting slide block can drive the sample platform module to lift so as to realize automatic focusing.
Further, the polarization analyzer module comprises a support frame which is formed by splicing and mounting a support piece, a first support rod, a machine leg, an adjusting rod, a support seat and a base together and is used for supporting the whole polarization analyzer module, a polarization analyzing module shell is mounted on the support frame, and a polarization analyzing connector, a shaft connector, a first motor connecting plate, a polarization analyzing stepping motor, a limiting rod, a limiting block, a polarization analyzing four-core aviation plug, a second motor connecting plate, a lead screw, a sliding block and a lead screw seat are arranged in the polarization analyzing module shell;
the limiting blocks are arranged on the second motor connecting plate and are positioned at the tail ends of the limiting rods, and the two limiting blocks are used for limiting the moving range of the limiting rods and limiting the horizontal displacement moving range of the first polarization detection stepping motor;
the offset detection stepping motor and the shaft connector are both provided with two, the first offset detection stepping motor is connected with the offset detection connector through the shaft connector, meanwhile, the first offset detection stepping motor is fixedly connected with a limiting rod, the tail end of the limiting rod is provided with a limiting structure, the horizontal displacement of the first offset detection stepping motor can be limited by matching with the limiting rod, the second offset detection stepping motor is installed on an offset detection module shell through a second motor connecting plate, meanwhile, the second offset detection stepping motor is sequentially connected with a screw rod and a screw rod seat through the shaft connector, a sliding block is connected onto the screw rod, the sliding block is connected with the first offset detection stepping motor through the first motor connecting plate, and the offset detection four-core aviation plug is used for connecting a cable to realize control over the offset detection stepping motor.
Further, the polarizer module comprises a containing structure consisting of a large bottom plate and a polarizing module shell, wherein a fixed connecting piece, a second supporting rod, a vertical plate, a first synchronous belt wheel, a synchronous belt adjusting plate, a synchronous belt, a polarizing ring, a polarizing mirror, an adjusting inner ring, a polarizing seat, an adjusting outer ring, a synchronous belt wheel seat, a second synchronous belt wheel, a polarizing stepping motor, a motor seat and a polarizing four-core aviation plug are arranged in the containing structure;
the polarizer module is installed on a base of the microscope through an adjusting fixing block, the adjusting fixing block is connected with a fixed connecting piece through a second supporting rod, one end of the second supporting rod is arranged outside a casing of the polarizing module, the other end of the second supporting rod penetrates through the side wall of the casing of the polarizing module and is arranged inside the casing of the polarizing module, one end of a vertical plate is installed on a large bottom plate, the other end of the vertical plate is connected with a motor base, a polarizing stepping motor is installed on the motor base, the output end of the polarizing stepping motor drives a first synchronous belt wheel to rotate, and the first synchronous belt wheel is connected with the second synchronous belt wheel through a synchronous belt;
the synchronous belt adjusting plate is installed on the large base plate, the vertical plate is installed on the synchronous belt adjusting plate, the vertical plate can adjust the position of the synchronous belt adjusting plate, the motor base is connected to the vertical plate, the polarizing stepping motor is installed on the motor base, and the position of the polarizing stepping motor can be adjusted by adjusting the position of the vertical plate on the synchronous belt adjusting plate;
the polarizing seat is fixedly connected to the large bottom plate, the adjusting outer ring is connected to the polarizing seat, the adjusting inner ring is rotatably connected with the adjusting outer ring, and the polarizing ring fixes the polarizing mirror on the adjusting inner ring through pressure; the synchronous pulley seat is fixedly connected with the adjusting inner ring, and the second synchronous pulley is fixedly connected with the synchronous pulley seat; the polarization four-core aviation plug is used for connecting a cable to realize the control of the polarization stepping motor.
Further, the sample stage module comprises an X-axis driving mechanism, a Y-axis driving mechanism, a sample stage flat plate, a sample hole and a sample placing groove; the X-axis driving mechanism and the Y-axis driving mechanism are vertically arranged and used for driving the sample stage flat plate to move along the X axis or the Y axis; the sample platform is provided with a sample placing groove on the flat plate, and a sample hole is formed in the middle of the sample placing groove.
A multi-dimensional rock slice digital automatic acquisition method utilizes the multi-dimensional rock slice digital automatic acquisition system, and comprises the following steps:
s1: collecting a single-polarization image;
s2: collecting an orthogonal polarized light image;
s3: collecting a fluorescence image;
s4: and image splicing and calling.
Further, the step S1 includes:
1) Placing rock slice samples to be detected into sample placing grooves on a sample table module, wherein the number of the rock slice samples is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module to move horizontally, so that the analyzer is pulled out; light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light enters the microscope objective lens after passing through the rock slice sample and then reaches the imaging module of the microscope; controlling the sample stage module to move horizontally by the computer so that the upper left corner of the rock slice sample appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
3) The computer controls a polarizer in the polarizer module to rotate to an initial angle, the imaging module shoots a first single-polarization image at the same time, and then the polarizer is controlled to rotate at fixed angle intervals to continuously shoot a plurality of single-polarization images; the photographed images are stored in a database;
4) The computer controls the sample table module to move according to the magnification factor, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at fixed angle intervals, and continuously shooting a plurality of single-polarization images; the photographed images are stored in a database; the sample stage module continues to move until the first rock slice completes all angle shots of all positions;
5) The computer controls the sample table module to move, so that a second rock slice moves to the visual field, and the steps 2) to 4) are repeated, and so on until all angles of all positions of all rock slice samples are shot;
the step S2 includes:
1) Placing rock slice samples to be detected into sample placing grooves on a sample table module, wherein the number of the rock slice samples is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module to move horizontally, so that the analyzer is inserted; light emitted by the light source passes through the polarizer and is converted into linearly polarized light, the linearly polarized light passes through the rock slice sample and then enters the microscope objective lens, and then passes through the analyzer and reaches the imaging module of the microscope, so that the orthogonal relationship between the polarizer and the analyzer is ensured; the computer controls the sample platform module to move horizontally, so that the upper left corner of the rock slice sample appears in the visual field of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
3) The computer controls the polarizer and the polarizer to rotate to an initial angle, so that the polarizer and the polarizer are in an orthogonal relation, the imaging module shoots a first single-polarization image, then the polarizer is controlled to rotate at a fixed angle interval, so that the polarizer and the polarizer are in the orthogonal relation, and multiple orthogonal polarization images are continuously shot; the photographed images are stored in a database;
4) The computer controls the sample stage module to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at intervals according to a fixed angle, ensuring that the polarizer and the analyzer are in an orthogonal relation, and continuously shooting a plurality of orthogonal polarized light images; the photographed images are stored in a database; the sample stage module continues to move until the first rock slice completes all angle shots of all positions;
5) Controlling the sample table module to move by the computer, so that a second rock slice moves to the visual field, repeating the steps 2) to 4), and so on until all angle shooting of all positions of all rock slice samples is completed;
the step S3 includes:
1) Placing rock slice samples to be detected into sample placing grooves on a sample table module, wherein the number of the rock slice samples is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module to move horizontally, so that the analyzer is pulled out; the light emitted by the fluorescence module reaches the rock slice sample through the reflection of the spectroscope, and the rock slice sample is excited to emit fluorescence; fluorescence emitted by the rock slice sample enters a microscope objective lens and then reaches an imaging module of a microscope; controlling the sample stage module to move horizontally by the computer so that the upper left corner of the rock slice sample appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
3) The computer controls the sample stage module to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; continuously shooting a plurality of fluorescence images, and storing the shot images in a database; the sample stage module continuously moves until the first rock slice finishes shooting all positions;
4) Controlling the sample table module to move by the computer, so that a second rock slice moves to the visual field, repeating the steps 2) to 3), and so on until the shooting of all positions of all rock slice samples is completed;
the step S4 includes:
1) The computer numbers each of the photographed single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image;
2) The computer carries out image preprocessing on adjacent single-polarization microscopic images, then carries out image splicing, and splices the single-polarization microscopic images corresponding to the same rock slice sample into a complete high-definition large image; the computer carries out image preprocessing on adjacent orthogonal polarization microscopic images, then carries out image splicing, and splices the orthogonal polarization microscopic images corresponding to the same rock slice sample into a complete high-definition large image; the computer carries out image preprocessing on adjacent fluorescence microscopic images, then carries out image splicing, and splices the fluorescence microscopic images corresponding to the same rock slice sample into a complete high-definition large image;
3) The computer stores the single-polarization microscopic image, the orthogonal-polarization microscopic image and the fluorescence microscopic image of the same rock slice sample as a group in a database; when the computer calls the images of the database, the thumbnail of the high-definition large image is displayed on a computer display screen, a selection box is displayed on the thumbnail, and the high-definition image at the position selected by the selection box is displayed in the center of the display screen; and the selection square frame is dragged, and the high-definition image displayed on the display screen moves along with the selection square frame, so that convenient observation is realized.
Further, the image stitching includes:
image processing:
firstly, randomly selecting 10-20 pixel points in each shot image, and then calculating the intermediate value of a brightness channel of the selected pixel points; then obtaining the average value of the brightness intermediate values of all the images to be spliced;
then, averaging the brightness of all the images, namely adjusting the brightness of each image to ensure that the brightness median of each image is equal to the average value of the brightness median of all the images;
image segmentation:
dividing each image to be spliced into 10000 sub-images, and then calculating the texture characteristics of each sub-image;
and then comparing the texture characteristics of the edge sub-images of the adjacent images to be spliced, and marking the sub-images with the same texture characteristics as the same sub-images.
Image splicing:
splicing the adjacent images to be spliced by taking the same sub-images marked in the adjacent images to be spliced as an alignment reference; and by parity of reasoning, completing the splicing of all the images to be spliced and obtaining the large-view spliced image.
Compared with the prior art, the digital automatic acquisition system and the acquisition method for the multi-dimensional rock slices have the following advantages:
(1) The polarizer module, the analyzer module and the sample stage module which are automatically controlled are combined with the microscope, and are automatically controlled by a computer, so that the rock slice samples are automatically fed and collected in batches for more than 6 times, and the rock slice photo collection working efficiency is improved;
(2) The invention designs a novel polarizer module, a novel analyzer module and a novel sample platform module, supports multi-angle rotary scanning of a full-automatic sheet sample in an orthogonal polarization mode and a single polarization mode, and scans a photographing region and follows a rotation angle; supporting the rotation correction of a large image of a flake sample, and reversely rotating the large image by a corresponding angle to ensure that the shape characteristics of target particles are consistent;
(3) The image splicing method provided by the invention is used for rapidly segmenting and calculating the edge texture characteristics aiming at the characteristics of the polarized light microscopic image, is high in splicing speed and splicing precision, supports full-automatic splicing of hundreds of thousands of images, and can realize image storage with hundreds of G capacity by adopting a layering and blocking scheme;
(4) The invention adopts three-level access strategies of screen cache, high-speed cache and pyramid images for the ultrahigh-resolution images, and can realize the quick display of the ultrahigh-resolution images under different resolutions;
(5) The system supports the block output to be a universal image format on the premise of not sacrificing the resolution, and simultaneously supports the thumbnail output of a full-looking image; and automatic fusion of image overlapping areas is supported, the conditions of uneven illumination, different definition and the like are solved, and a perfect large-view spliced image is obtained.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of the overall architecture of the system of the present invention;
FIG. 2 is a schematic diagram of polarization detection according to the present invention;
FIG. 3 is a schematic view of the microscope of the present invention;
FIG. 4 is a schematic view of the structure of the microscope of the present invention;
FIG. 5 is a schematic structural diagram of an analyzer module according to the present invention;
FIG. 6 is a schematic structural diagram of a polarizer module according to the present invention;
fig. 7 is a schematic structural diagram of the sample stage module according to the present invention.
Description of reference numerals:
1. an analyzer module; 2. a polarizer module; 3. a sample stage module; 4. a CCD sensor; 5. an eyepiece; 501. an eyepiece interface; 6. an objective lens; 601. an objective lens interface; 7. a fluorescent illumination source; 8. a sample; 9. a beam splitter; 10. an illumination light source;
101. a polarization analyzing connector; 102. a shaft coupling; 103. a first motor connecting plate; 104. a polarization-detecting stepping motor; 105. a limiting rod; 106. a limiting block; 107. a polarization-detecting module housing; 108. analyzing a four-core aviation plug; 109. a second motor connecting plate; 110. a screw rod; 111. a support member; 112. a first support bar; 113. a machine leg; 114. adjusting a rod; 115. a supporting base; 116. a base; 117. a slider; 118. a screw base;
201. fixing the connecting piece; 202. a second support bar; 203. a vertical plate; 204. a first timing pulley; 205. a synchronous belt adjusting plate; 206. a synchronous belt; 207. a large base plate; 208. a bias ring is formed; 209. a polarizer 210, an adjusting inner ring; 211. a polarizing seat; 212. a polarizing module housing; 213. adjusting the outer ring; 214. a synchronous pulley seat; 215. a second timing pulley; 216. a polarizing stepping motor; 217. a motor base; 218. polarizing a four-core aviation plug; 219. adjusting the fixed block;
301. an X-axis drive mechanism; 302. a Y-axis drive mechanism; 303. a sample stage flat plate; 304. a sample placement groove; 305. and (4) a sample hole.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
Example 1:
with reference to fig. 1, the present invention is a digital automatic acquisition system for multidimensional rock slices, comprising a computer, a database, an image processing module, a microscope module and an acquisition module;
the computer is connected with the image processor, the database, the microscope module and the acquisition module;
the microscope module comprises a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected with the imaging module, the fluorescence module, the light source module and the objective lens module; the microscope control module controls the imaging module, the fluorescence module, the light source module and the objective lens module to work;
the acquisition module comprises an acquisition controller, a polarization analyzer module 1, a sample stage module 3 and a polarizer module 2; the acquisition controller is connected with the analyzer module 1, the sample stage module 3 and the polarizer module 2, and controls the movement and the work of the analyzer module 1, the sample stage module 3 and the polarizer module 2;
as shown in fig. 2, the acquisition module and the microscope module work in a matching manner, an imaging module and a light source module are arranged in the microscope module, the acquisition module can convert light emitted by the light source module into linearly polarized light for polarized light microscopic detection, and can realize switching between single-polarization detection and orthogonal-polarization detection and detection of orthogonal polarized light with different angles;
specifically, the polarizer module 2 is provided with a polarizer and a stepping motor for driving the polarizer to rotate, and the polarizer converts light emitted by the light source module into linearly polarized light so as to be used for polarized light microscopic detection; the sample stage module 3 is used for placing a plurality of rock slices and driving the rock slices to move along an X axis and a Y axis so as to realize full-automatic sample introduction of the rock slices, the sample stage module 3 is arranged on a lifting slide block 117 of the microscope, and the lifting slide block 117 can drive the sample stage module 3 to lift so as to realize automatic focusing; the analyzer module 1 is provided with an analyzer and a stepping motor for driving the analyzer to translate and rotate, and the analyzer translates to realize the switching between single-polarization detection and orthogonal-polarization detection; the rotation of the analyzer is matched with the rotation of the polarizer to jointly realize the detection of orthogonal polarized light with different angles;
the imaging module shoots a high-definition polarization microscopic image of the rock slice and sends the high-definition polarization microscopic image to the computer; the image processing module automatically splices the high-definition polarized light microscopic images in the computer to obtain spliced super-large images, and the super-large images are stored in a database.
As shown in fig. 3 and 4, in the microscope module, the imaging module, the fluorescence module, the objective lens module and the light source module are sequentially arranged from top to bottom; in the description of the present invention, it is to be understood that the terms "upper", "lower", "inside", "outside", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
An illumination light source 10 is arranged in the light source module, and light emitted by the illumination light source 10 passes through the sample 8 to be detected and then is transmitted to the objective lens module;
light entering from the objective lens module is transmitted to the imaging module after passing through an inner microscopic light path of the microscope so as to realize microscopic imaging;
the imaging module is internally provided with a CCD sensor 4 for shooting a high-definition microscopic image of the rock slice; the CCD sensor 4 is provided with an imaging module interface which is used for installing an imaging module;
an eyepiece module is also arranged between the imaging module and the fluorescence module, and is provided with an eyepiece interface 501 and a replaceable eyepiece 5;
a fluorescence illumination light source 7 is arranged in the fluorescence module to emit a fluorescence excitation light source to a sample 8 to be detected, and fluorescence emitted by the sample 8 is transmitted to the ocular lens module and the imaging module through the objective lens;
the objective module is provided with an objective interface 601 and a switchable eyepiece 6 to enable switching of the eyepiece 6 of different magnifications. Eyepiece 6 is provided with rotational position sensor for rotatory switching mode in, and the microscope module is connected to the computer, and the computer can obtain the eyepiece 6 magnification that the microscope selected in real time.
The polarizer module 2 is arranged between the light source module and the objective lens module and is used for converting light emitted by the light source module into linearly polarized light; the polarizer module 2 is provided with a polarizer and a stepping motor for driving the polarizer to rotate, and the polarizer can convert the light emitted by the light source module into linearly polarized light for polarized light microscopic detection;
the sample stage module 3 is arranged between the polarizer module 2 and the objective lens module and is used for placing the rock slice for microscopic detection; the device can be used for placing a plurality of rock slices and driving the rock slices to move along an X axis and a Y axis so as to realize full-automatic sample introduction of the rock slices;
the analyzer module 1 is arranged between the objective lens module and the imaging module and used for realizing orthogonal polarization detection in cooperation with the polarizer module 2. Specifically, the analyzer module 1 is provided with an analyzer and a stepping motor for driving the analyzer to translate and rotate, and the analyzer translates to realize the switching between single-polarization detection and orthogonal-polarization detection; the rotation of the analyzer is matched with the rotation of the polarizer to jointly realize the detection of orthogonal polarized lights with different angles.
As shown in fig. 5, the analyzer module 1 includes a support frame formed by splicing and mounting a support member 111, a first support rod 112, a machine leg 113, an adjusting rod 114, a support seat 115 and a base 116, and is used for supporting the whole analyzer module 1, an analyzer module housing 107 is mounted on the support frame, and an analyzer connector 101, a shaft connector 102, a first motor connecting plate 103, an analyzer stepping motor 104, a limit rod 105, a limit block 106, an analyzer four-core aviation plug 108, a second motor connecting plate 109, a screw rod 110, a slider 117 and a screw rod seat 118 are arranged in the analyzer module housing 107;
the two limiting blocks 106 are respectively arranged at two sides of the tail end of the limiting rod 105 and used for limiting the moving range of the limiting rod 105 and limiting the horizontal displacement moving range of the first polarization detection stepping motor 104;
the two polarization detection stepping motors 104 and the two shaft connectors 102 are arranged, the first polarization detection stepping motor 104 is connected with the polarization detection connector 101 through the shaft connector 102, meanwhile, the first polarization detection stepping motor 104 is fixedly connected with the limiting rod 105, the tail end of the limiting rod 105 is provided with a limiting structure, the limiting structure can be matched with the limiting rod 106 to limit the horizontal displacement of the first polarization detection stepping motor 104, the second polarization detection stepping motor 104 is installed on a polarization detection module shell 107 through a second motor connecting plate 109, meanwhile, the second polarization detection stepping motor 104 is sequentially connected with a screw rod 110 and a screw rod seat 118 through the shaft connector 102, a sliding block 117 is connected onto the screw rod 110 in a sliding mode, the sliding block 117 is connected with the first polarization detection stepping motor 104 through the first motor connecting plate 103, and along with the horizontal movement of the sliding block 117 on the screw rod 110, the first motor connecting plate 103 can drive the first polarization detection stepping motor 104 to realize the horizontal movement, and therefore the insertion and the extraction of the polarization detector are realized. The polarization-detecting four-core aviation plug 108 is used for connecting a cable to realize the control of the polarization-detecting stepping motor 104. In the description of the present invention, it is to be understood that the terms "first", "second", and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature.
As shown in fig. 6, the polarizer module 2 includes a containing structure composed of a large bottom plate 207 and a polarizing module housing 212, and a fixed connector 201, a second supporting rod 202, a vertical plate 203, a first synchronous pulley 204, a synchronous belt adjusting plate 205, a synchronous belt 206, a polarizing ring 208, a polarizing mirror 209, an adjusting inner ring 210, a polarizing seat 211, an adjusting outer ring 213, a synchronous pulley seat 214, a second synchronous pulley 215, a polarizing stepping motor 216, a motor seat 217 and a polarizing four-core aviation plug 218 are arranged in the containing structure;
the polarizer module 2 is installed on a base 116 of the microscope through an adjusting fixing block 219, the adjusting fixing block 219 is connected with a fixed connecting piece 201 through a second supporting rod 202, one end of the second supporting rod 202 is arranged outside a polarization module shell 212, the other end of the second supporting rod passes through the side wall of the polarization module shell 212 and is arranged inside the polarization module shell 212, one end of a vertical plate 203 is installed on a large bottom plate 207, the other end of the vertical plate is connected with a motor base 217, a polarization stepping motor 216 is installed on the motor base 217, the output end of the polarization stepping motor 216 drives a first synchronous pulley 204 to rotate, the first synchronous pulley 204 is connected with a second synchronous pulley 215 through a synchronous belt 206, and therefore the polarization stepping motor 216 can drive the second synchronous pulley 215 to rotate;
hold-in range regulating plate 205 is used for adjusting the contact of first hold-in range pulley 204 and hold-in range 206, concretely, hold-in range regulating plate 205 is installed on big bottom plate 207, riser 203 is installed on hold-in range regulating plate 205, and riser 203 can carry out the regulation of position on hold-in range regulating plate 205, motor cabinet 217 is connected on riser 203, it installs on motor cabinet 217 to play inclined to one side step motor 216, thereby realize adjusting inclined to one side step motor 216's position through adjusting the position of riser 203 on hold-in range regulating plate 205, further adjust the elasticity degree of hold-in range 206 in order to realize adjusting the contact of first hold-in range pulley 204 and hold-in range 206.
The polarizing ring 208, the adjusting inner ring 210, the polarizing seat 211, the adjusting outer ring 213 and the synchronous pulley seat 214 are used for realizing connection and installation between the second synchronous pulley 215 and the polarizing mirror 209, specifically, the polarizing seat 211 is fixedly connected to the large base plate 207, the adjusting outer ring 213 is connected to the polarizing seat 211, the adjusting inner ring 210 and the adjusting outer ring 213 are rotatably connected, and the polarizing mirror 209 is fixed on the adjusting inner ring 210 by the polarizing ring 208 through pressure; the synchronous pulley seat 214 is fixedly connected with the adjusting inner ring 210, and the second synchronous pulley 215 is fixedly connected with the synchronous pulley seat 214; when the synchronous belt 206 rotates, the second synchronous pulley 215 and the synchronous pulley seat 214 can be driven to synchronously rotate; further driving the polarizer 209 installed on the adjusting inner ring 210 to rotate. The polarizing quad pin 218 is used to connect cables to control the polarizing stepper motor 216.
As shown in fig. 7, the sample stage module 3 includes an X-axis drive mechanism 301, a Y-axis drive mechanism 302, a sample stage flat plate 303, a sample hole 305, and a sample placement groove 304; the X-axis driving mechanism 301 and the Y-axis driving mechanism 302 are vertically arranged, and the X-axis driving mechanism 301 and the Y-axis driving mechanism 302 are used for driving the sample table flat plate 303 to move along the X axis or the Y axis; the sample table flat plate 303 is provided with a sample placing groove 304, and a sample hole 305 is formed in the middle of the sample placing groove 304.
Example 2:
a multi-dimensional rock slice digital automatic acquisition method utilizes the multi-dimensional rock slice digital automatic acquisition system, and comprises the following steps:
s1: collecting a single-polarization image;
1-1) placing rock slice samples 8 to be detected into sample placing grooves 304 on a sample table module 3, wherein the number of the rock slice samples 8 is 4-6, and the rock slice samples are placed side by side;
1-2) controlling the analyzer module 1 to move horizontally by a computer to pull out the analyzer; light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light enters the microscope eyepiece 6 after passing through the rock slice sample 8 and then reaches the imaging module of the microscope; the computer controls the sample stage module 3 to move horizontally so that the upper left corner of the rock slice sample 8 appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
1-3) the computer controls the polarizer in the polarizer module 2 to rotate to an initial angle, the imaging module shoots a first single polarized light image at the same time, and then the polarizer is controlled to rotate at fixed angle intervals to continuously shoot a plurality of single polarized light images; the photographed images are stored in a database;
1-4) the computer controls the sample stage module 3 to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at fixed angle intervals, and continuously shooting a plurality of single-polarization images; the photographed images are stored in a database; the sample stage module 3 continues to move until the first rock slice completes all angle shots of all positions;
1-5) controlling the sample stage module 3 to move by the computer, so that the second rock slice moves to the visual field, repeating the steps 1-2) to 1-4), and so on until all angle shooting of all positions of all rock slice samples 8 is completed;
s2: collecting an orthogonal polarized light image;
2-1) placing the rock slice samples 8 to be detected into the sample placing grooves 304 on the sample table module 3, wherein the number of the rock slice samples 8 is 4-6, and the rock slice samples are placed side by side;
2-2) the computer controls the analyzer module 1 to move horizontally so that the analyzer is inserted; light emitted by the light source is converted into linearly polarized light after passing through the polarizer, the linearly polarized light enters the microscope eyepiece 6 after passing through the rock slice sample 8, and then passes through the analyzer to reach an imaging module of the microscope, so that the polarizer and the analyzer are in an orthogonal relation; the computer controls the sample stage module 3 to move horizontally so that the upper left corner of the rock slice sample 8 appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module, and the imaging module can be ensured to be a clear microscopic image;
2-3) the computer controls the analyzer and the polarizer to rotate to an initial angle, so that the polarizer and the analyzer are in an orthogonal relation, the imaging module shoots a first single-polarization image, then the polarizer is controlled to rotate at a fixed angle interval, so that the polarizer and the analyzer are in an orthogonal relation, and multiple orthogonal polarization images are continuously shot; the photographed images are stored in a database;
2-4) the computer controls the sample stage module 3 to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at intervals according to a fixed angle, ensuring that the polarizer and the analyzer are in an orthogonal relation, and continuously shooting a plurality of orthogonal polarized light images; the photographed images are stored in a database; the sample stage module 3 continues to move until the first rock slice completes all angle shots of all positions;
2-5) controlling the sample stage module 3 to move by the computer, so that the second rock slice moves to the visual field, repeating the steps 2-2) to 2-4), and so on until all the angle shooting of all the positions of all the rock slice samples 8 is completed;
s3: collecting a fluorescence image;
3-1) placing the rock slice samples 8 to be detected into the sample placing grooves 304 on the sample table module 3, wherein the number of the rock slice samples 8 is 4-6, and the rock slice samples are placed side by side;
3-2) the computer controls the analyzer module 1 to move horizontally, so that the analyzer is pulled out; the light emitted by the fluorescence module reaches the rock slice sample 8 through the reflection of the spectroscope 9, and the rock slice sample 8 is excited to emit fluorescence; fluorescence emitted by the rock slice sample 8 enters the microscope eyepiece 6 and then reaches the imaging module of the microscope; the computer controls the sample stage module 3 to move horizontally so that the upper left corner of the rock slice sample 8 appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
3-3) the computer controls the sample stage module 3 to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; continuously shooting a plurality of fluorescence images, and storing the shot images in a database; the sample stage module 3 continues to move until the first rock slice completes the shooting of all positions;
3-4) controlling the sample stage module 3 to move by the computer, so that the second rock slice moves to the visual field, repeating the steps 3-2) to 3-3), and so on until the shooting of all positions of all rock slice samples 8 is completed;
s4: and image splicing and calling.
4-1) numbering each single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image which are shot by a computer;
4-2) carrying out image preprocessing on adjacent single polarization microscopic images by a computer, then carrying out image splicing, and splicing the single polarization microscopic images corresponding to the same rock slice sample 8 into a complete high-definition large image; the computer carries out image preprocessing on adjacent orthogonal polarization microscopic images, then carries out image splicing, and splices the orthogonal polarization microscopic images corresponding to the same rock slice sample 8 into a complete high-definition large image; the computer carries out image preprocessing on adjacent fluorescence microscopic images, then carries out image splicing, and splices the fluorescence microscopic images corresponding to the same rock slice sample 8 into a complete high-definition large image;
4-3) storing the single-polarization microscopic image, the orthogonal-polarization microscopic image and the fluorescence microscopic image of the same rock slice sample 8 as a group in a database by a computer; when the computer calls the images of the database, the thumbnail of the high-definition large image is displayed on a computer display screen, a selection box is displayed on the thumbnail, and the high-definition image at the position selected by the selection box is displayed in the center of the display screen; and the selection square frame is dragged, and the high-definition image displayed on the display screen moves along with the selection square frame, so that convenient observation is realized.
The image splicing method comprises the following steps:
image processing:
firstly, randomly selecting 10-20 pixel points in each shot image, and then calculating the intermediate value of a brightness channel of the selected pixel points; then obtaining the average value of the brightness intermediate values of all the images to be spliced;
then, brightness averaging is carried out on all the images, namely, the brightness of each image is adjusted, so that the brightness middle value of each image is equal to the average value of the brightness middle values of all the images;
image segmentation:
dividing each image to be spliced into 10000 sub-images, and then calculating the texture characteristics of each sub-image;
and then comparing the texture characteristics of the edge sub-images of the adjacent images to be spliced, and marking the sub-images with the same texture characteristics as the same sub-images.
Image splicing:
splicing the adjacent images to be spliced by taking the same sub-images marked in the adjacent images to be spliced as an alignment reference; and by parity of reasoning, completing the splicing of all the images to be spliced and obtaining the large-visual-field spliced image.
Example 3:
the microscope system adopts a Leica DM2500P/DM2700P series microscope or a DM4500P microscope, the microscope is provided with a 6X M25 objective rotating disc, can be adjusted and memorized, and the computer automatically identifies the ocular lens multiple of 6 after being connected with the computer; the light source is a 12V-100W halogen lamp or an LED light source, has the functions of automatic light intensity tracking and constant color temperature, ensures that the color temperature and the brightness of images shot in any lighting environment are constant, and reduces the calculation amount of subsequent image preprocessing.
Installing an independently designed acquisition controller, an analyzer module 1, a sample stage module 3 and a polarizer module 2 into a microscope system, and installing independently designed matched control software in a computer; acquiring a single-polarization image by using the method in the embodiment 2, selecting 6 rock slices, and automatically acquiring the image;
the single-polarization detection and the orthogonal-polarization detection are automatically controlled by the computer-controlled microscope, the polarizer module 2, the analyzer module 1 and the sample stage module 3, and the whole process does not need manual guard; the computer controls the polarizer module 2 to automatically rotate at certain angle intervals, and controls the analyzer module 1 to automatically insert, pull out or rotate according to the detection requirement. The computer controls the microscope to automatically identify the magnification factor, identify the position coordinate of the sample stage, identify the brightness of the shot image and automatically acquire and store the image; a plurality of rock slices are placed on the sample table module 3, and continuous collection is automatically carried out without any manual intervention.
The device of the invention can be used for singly detecting single polarization or orthogonal polarization, and can also be used for continuously detecting single polarization and orthogonal polarization.
When the single-polarization and orthogonal-polarization continuous detection is carried out, firstly, image acquisition of single-polarization detection is carried out, then working parameters of other equipment are kept unchanged, the analyzer module 1 is directly controlled to be automatically inserted, and then orthogonal-polarization images are directly acquired; therefore, the simultaneous continuous acquisition of single-polarization detection and orthogonal-polarization detection can be realized, and the time for focusing and position adjustment is saved.
As described above, the sample stage module 3 of the present invention can be automatically lifted under the control of a computer, automatically adjust the position of the Z axis by using the lifting freedom of the microscope module, and automatically focus when detecting in cooperation with the automatic focusing function of the microscope without manual adjustment.
Furthermore, the microscope is also provided with an insertion opening for a gypsum test board and a mica test board, and the gypsum test board or the mica test board can be inserted according to the requirement so as to improve the display effect of the polarized light microscopic detection; the gypsum test board is made of crystal wafer, and the optical path difference is 530nm-550nm. The optical path difference of the mica test plate is about one fourth of the wavelength of yellow light, namely about 147nm, and first-order gray white interference color is presented between the crossed polarizers. After adding the mica test plate to the flakes, the interference color order of the flakes is raised and lowered by about one color order in the order of the chromatographic chart. The test plate is more suitable for ore slices with higher interference color.
Acquiring 128 × 128=16384 high-definition microscopic images for each rock slice, wherein the acquisition time of each image is 0.2s, the acquisition interval is 0.2s, the acquisition of each rock slice is completed in less than 2 hours, and about 12 hours are required for acquiring 98304 images for 6 rock slices; the system supports automatic splicing of a hundred thousand images to the maximum extent; and automatically splicing the collected microscopic images of each rock slice to obtain rock slice images with full samples, ultra-large breadth and ultra-high resolution, shooting and splicing the rock slice images in the shooting process, and completing the splicing immediately after the shooting is completed.
After splicing, storing the large image in a computer database, and displaying a thumbnail when the large image is used so as to improve the browsing speed; after the detailed position is selected, displaying a high-definition large image of the corresponding position on a display; and position marking and dragging browsing are supported in the display process.
Example 4:
the microscope system adopts a Leica DM2500P/DM2700P series microscope, an independently designed acquisition controller, an analyzer module 1, a sample stage module 3 and a polarizer module 2 are installed in the microscope system, and independently designed matched control software is installed in a computer; acquiring orthogonal polarization images by using the method of the embodiment, selecting 6 rock slices, and automatically acquiring the images;
50 multiplied by 50=2500 high-definition microscopic images are acquired by each rock slice, the acquisition time of each image is 0.5s, the acquisition interval is 0.5s, each rock slice only needs less than 1 hour after the acquisition is completed, and 15000 images are acquired by 6 rock slices in total, which needs about 6 hours; the system supports automatic splicing of a hundred thousand images to the maximum extent; and automatically splicing the collected microscopic images of each rock slice to obtain rock slice images with full samples, ultra-large breadth and ultra-high resolution, shooting and splicing the rock slice images in the shooting process, and completing the splicing immediately after the shooting is completed.
After splicing, storing the large image in a computer database, and displaying a thumbnail when the large image is used so as to improve the browsing speed; after the detailed position is selected, displaying a high-definition large image of the corresponding position on a display; and position marking and dragging browsing are supported in the display process.
It is worth pointing out that the acquisition time in the embodiment is directly related to the number of the microscopic image splices, and meanwhile, the acquisition time and the acquisition interval of each image can be considered to be adjusted according to the imaging effect of the equipment, and the acquisition interval can be set to 0.1s and the acquisition time is set to 0.1s at the fastest speed; due to the fact that the automatic splicing of hundreds of thousands of images is supported, theoretically, a single rock slice can shoot hundreds of thousands of images at most; in actual use, the required number of shots can be selected according to requirements, and the number of shots is generally required to be more than 400.
After the spliced images are stored, the digital storage of the rock slices can be realized, and the deformation caused by environmental factors during the long-term storage of the sample is avoided; meanwhile, the data is stored by adopting a digital technology, so that the convenience of data transmission and sharing can be greatly improved, and the help is provided for scientific research.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. The utility model provides a multidimension degree rock thin slice digital automatic acquisition system which characterized in that: the system comprises a computer, a database, an image processing module, a microscope module and an acquisition module;
the computer is connected with the image processor, the database, the microscope module and the acquisition module;
the acquisition module can convert light emitted by the light source module into linearly polarized light for polarized light microscopic detection, can realize the switching of single-polarization detection and orthogonal polarized light detection and realize the detection of orthogonal polarized light with different angles;
the imaging module shoots a high-definition polarization microscopic image of the rock slice and sends the high-definition polarization microscopic image to the computer; the image processing module automatically splices the high-definition polarized light microscopic images in the computer to obtain spliced super-large images, and the super-large images are stored in a database.
2. The digital automatic acquisition system of multidimensional rock slices as recited in claim 1, wherein: the microscope module comprises a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected with the imaging module, the fluorescence module, the light source module and the objective lens module, the imaging module, the fluorescence module, the objective lens module and the light source module are sequentially arranged from top to bottom, and meanwhile, the microscope control module controls the imaging module, the fluorescence module, the light source module and the objective lens module to work;
an illumination light source (10) is arranged in the light source module, and light emitted by the illumination light source (10) passes through a sample (8) to be detected and then is transmitted to the objective lens module;
light entering from the objective lens module is transmitted to the imaging module after passing through an inner microscopic light path of the microscope so as to realize microscopic imaging;
the imaging module is internally provided with a CCD sensor (4) for shooting a high-definition microscopic image of the rock slice; the CCD sensor (4) is provided with an imaging module interface which is used for installing an imaging module; an eyepiece module is also arranged between the imaging module and the fluorescence module, and is provided with an eyepiece interface (501) and a replaceable eyepiece (5);
a fluorescence illumination light source (7) is arranged in the fluorescence module to emit a fluorescence excitation light source to a sample (8) to be detected, and fluorescence emitted by the sample (8) is transmitted to the ocular lens module and the imaging module through the objective lens;
the objective module is provided with an objective interface (601) and a switchable eyepiece (6) to enable switching of the eyepiece (6) at different magnifications.
3. The digital automatic acquisition system of multidimensional rock slices as recited in claim 2, wherein: the acquisition module comprises an acquisition controller, a polarization analyzer module (1), a sample stage module (3) and a polarizer module (2); the acquisition controller is connected with the analyzer module (1), the sample stage module (3) and the polarizer module (2), and simultaneously controls the movement and the work of the analyzer module (1), the sample stage module (3) and the polarizer module (2);
the polarizer module (2) is arranged between the light source module and the objective lens module, the polarizer module (2) is provided with a polarizer and a stepping motor for driving the polarizer to rotate, and the polarizer can convert light emitted by the light source module into linearly polarized light for polarized light microscopic detection;
the sample table module (3) is arranged between the polarizer module (2) and the objective lens module and is used for placing a plurality of rock slices and driving the rock slices to move along an X axis and a Y axis so as to realize full-automatic sample introduction of the rock slices;
the analyzer module (1) is arranged between the objective lens module and the imaging module, the analyzer module (1) is provided with an analyzer and a stepping motor for driving the analyzer to translate and rotate, and the analyzer translates to realize switching of single-polarization detection and orthogonal-polarization detection; the rotation of the analyzer is matched with the rotation of the polarizer to jointly realize the detection of orthogonal polarized lights with different angles.
4. The digital automatic acquisition system of multidimensional rock slices as recited in claim 2, wherein: the ocular lens (6) is in a rotary switching mode, a rotary position sensor is arranged in the ocular lens, the microscope module is connected to a computer, and the computer can obtain the magnification factor of the ocular lens (6) selected by the microscope in real time; the sample stage module (3) is arranged on a lifting slide block (117) of the microscope, and the lifting slide block (117) can drive the sample stage module (3) to lift so as to realize automatic focusing.
5. The digital automatic acquisition system of multidimensional rock slices as recited in claim 3, wherein: the analyzer module (1) comprises a support frame which is formed by splicing and installing a support piece (111), a first support rod (112), a machine foot (113), an adjusting rod (114), a support base (115) and a base (116) and is used for supporting the whole analyzer module (1), an analyzer module shell (107) is installed on the support frame, and an analyzer connector (101), a shaft connector (102), a first motor connecting plate (103), an analyzer stepping motor (104), a limiting rod (105), a limiting block (106), an analyzer four-core aviation plug (108), a second motor connecting plate (109), a screw rod (110), a sliding block (117) and a screw rod seat (118) are arranged in the analyzer module shell (107);
the two limiting blocks (106) are arranged on the second motor connecting plate (109), the limiting blocks (106) are positioned at the tail ends of the limiting rods (105), and the two limiting blocks (106) are used for limiting the moving range of the limiting rods (105) and limiting the horizontal displacement moving range of the first polarization detection stepping motor (104);
the two polarization detection stepping motors (104) and the two shaft connectors (102) are arranged, the first polarization detection stepping motor (104) is connected with the polarization detection connector (101) through the shaft connector (102), meanwhile, the first polarization detection stepping motor (104) is fixedly connected with the limiting rod (105), the tail end of the limiting rod (105) is provided with a limiting structure, the limiting structure can be matched with the limiting rod (106) to limit the horizontal displacement of the first polarization detection stepping motor (104), the second polarization detection stepping motor (104) is installed on a polarization detection module shell (107) through a second motor connecting plate (109), meanwhile, the second polarization detection stepping motor (104) is sequentially connected with a screw rod (110) and a screw rod seat (118) through the shaft connector (102), a sliding block (117) is connected onto the screw rod (110), the sliding block (117) is connected with the first polarization detection stepping motor (104) through the first motor connecting plate (103), and a four-core aviation cable plug (108) for connecting and achieving control over the polarization detection stepping motor (104).
6. The digital automatic acquisition system of multidimensional rock slices as recited in claim 5, wherein: the polarizer module (2) comprises a containing structure consisting of a large bottom plate (207) and a polarizing module shell (212), wherein a fixed connecting piece (201), a second supporting rod (202), a vertical plate (203), a first synchronous pulley (204), a synchronous belt adjusting plate (205), a synchronous belt (206), a polarizing ring (208), a polarizing mirror (209), an adjusting inner ring (210), a polarizing seat (211), an adjusting outer ring (213), a synchronous pulley seat (214), a second synchronous pulley (215), a polarizing stepping motor (216), a motor seat (217) and a polarizing four-core aviation plug (218) are arranged in the containing structure;
the polarizer module (2) is installed on a microscope base (116) through an adjusting fixing block (219), the adjusting fixing block (219) is connected with a fixed connecting piece (201) through a second supporting rod (202), one end of the second supporting rod (202) is arranged outside a polarizing module shell (212), the other end of the second supporting rod penetrates through the side wall of the polarizing module shell (212) and is arranged inside the polarizing module shell (212), one end of a vertical plate (203) is installed on a large bottom plate (207), the other end of the vertical plate is connected with a motor base (217), a polarizing stepping motor (216) is installed on the motor base (217), the output end of the polarizing stepping motor (216) drives a first synchronous belt pulley (204) to rotate, and the first synchronous belt pulley (204) is connected with a second synchronous belt pulley (215) through a synchronous belt (206);
the synchronous belt adjusting plate (205) is installed on the large bottom plate (207), the vertical plate (203) is installed on the synchronous belt adjusting plate (205), the vertical plate (203) can adjust the position on the synchronous belt adjusting plate (205), the motor base (217) is connected to the vertical plate (203), the polarizing stepping motor (216) is installed on the motor base (217), and the position of the polarizing stepping motor (216) can be adjusted by adjusting the position of the vertical plate (203) on the synchronous belt adjusting plate (205);
the polarizing seat (211) is fixedly connected to the large base plate (207), the adjusting outer ring (213) is connected to the polarizing seat (211), the adjusting inner ring (210) is rotatably connected with the adjusting outer ring (213), and the polarizing ring (208) fixes the polarizing mirror (209) to the adjusting inner ring (210) through pressure; the synchronous pulley seat (214) is fixedly connected with the adjusting inner ring (210), and the second synchronous pulley (215) is fixedly connected with the synchronous pulley seat (214); the polarization four-core aviation plug (218) is used for connecting a cable to realize control over the polarization stepping motor (216).
7. The digital automatic acquisition system of multidimensional rock slices as recited in claim 1, wherein: the sample table module (3) comprises an X-axis driving mechanism (301), a Y-axis driving mechanism (302), a sample table flat plate (303), a sample hole (305) and a sample placing groove (304); the X-axis driving mechanism (301) and the Y-axis driving mechanism (302) are vertically arranged, and the X-axis driving mechanism (301) and the Y-axis driving mechanism (302) are used for driving the sample stage flat plate (303) to move along the X axis or the Y axis; a sample placing groove (304) is arranged on the sample platform flat plate (303), and a sample hole (305) is arranged in the middle of the sample placing groove (304).
8. A digitalized automatic acquisition method for multidimensional rock slices, which utilizes the digitalized automatic acquisition system for multidimensional rock slices of any one of claims 3-7, and is characterized by comprising the following steps:
s1: collecting a single-polarization image;
s2: collecting an orthogonal polarized light image;
s3: collecting a fluorescence image;
s4: and image splicing and calling.
9. The digital automatic acquisition method of the multidimensional rock slice as claimed in claim 8, characterized in that:
the step S1 includes:
1) Placing rock slice samples (8) to be tested into sample placing grooves (304) on a sample table module (3), wherein the number of the rock slice samples (8) is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module (1) to move horizontally, so that the analyzer is pulled out; light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light enters the microscope eyepiece (6) after passing through the rock slice sample (8) and then reaches the imaging module of the microscope; computer-controlled horizontal movement of the sample stage module (3) such that the upper left corner of the rock lamella sample (8) appears in the field of view of the imaging module;
the computer carries out automatic focusing according to the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
3) The computer controls a polarizer in the polarizer module (2) to rotate to an initial angle, the imaging module shoots a first single polarized light image, and then the polarizer is controlled to rotate at fixed angle intervals to continuously shoot a plurality of single polarized light images; the photographed images are stored in a database;
4) The computer controls the sample stage module (3) to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at fixed angle intervals, and continuously shooting a plurality of single-polarization images; the photographed images are stored in a database; the sample stage module (3) continuously moves until the first rock slice finishes all-angle shooting at all positions;
5) The computer controls the sample table module (3) to move, so that the second rock slice moves to the visual field, and the steps 2) to 4) are repeated, and the like until all angle shooting of all positions of all rock slice samples (8) is completed;
the step S2 includes:
1) Placing the rock slice samples (8) to be tested into sample placing grooves (304) on a sample table module (3), wherein the number of the rock slice samples (8) is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module (1) to move horizontally, so that the analyzer is inserted; light emitted by the light source is converted into linearly polarized light after passing through the polarizer, the linearly polarized light enters an eyepiece (6) of the microscope after passing through a rock slice sample (8), and then passes through the analyzer to reach an imaging module of the microscope, so that the polarizer and the analyzer are ensured to be in an orthogonal relation; computer-controlled horizontal movement of the sample stage module (3) such that the upper left corner of the rock lamella sample (8) appears in the field of view of the imaging module; the computer carries out automatic focusing according to the real-time image of the imaging module, and the imaging module can be ensured to be a clear microscopic image;
3) The computer controls the polarizer and the polarizer to rotate to an initial angle, so that the polarizer and the polarizer are in an orthogonal relation, the imaging module shoots a first single-polarization image, then the polarizer is controlled to rotate at a fixed angle interval, so that the polarizer and the polarizer are in the orthogonal relation, and multiple orthogonal polarization images are continuously shot; the photographed images are stored in a database;
4) The computer controls the sample stage module (3) to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; then controlling the polarizer to rotate at intervals according to a fixed angle, ensuring that the polarizer and the analyzer are in an orthogonal relation, and continuously shooting a plurality of orthogonal polarized light images; the photographed images are stored in a database; the sample stage module (3) continuously moves until the first rock slice finishes all-angle shooting at all positions;
5) The computer controls the sample table module (3) to move, so that the second rock slice moves to the visual field, and the steps 2) to 4) are repeated, and the like until all angle shooting of all positions of all rock slice samples (8) is completed;
the step S3 includes:
1) Placing rock slice samples (8) to be tested into sample placing grooves (304) on a sample table module (3), wherein the number of the rock slice samples (8) is 4-6, and the rock slice samples are placed side by side;
2) The computer controls the analyzer module (1) to move horizontally, so that the analyzer is pulled out; the light emitted by the fluorescence module reaches the rock slice sample (8) through the reflection of the spectroscope (9) to excite the rock slice sample (8) to emit fluorescence; fluorescence emitted by the rock slice sample (8) enters an ocular lens (6) of the microscope and then reaches an imaging module of the microscope; computer-controlled horizontal movement of the sample stage module (3) such that the upper left corner of the rock lamella sample (8) appears in the field of view of the imaging module; the computer carries out automatic focusing according to the real-time image of the imaging module, and the imaging module can be ensured to be a clear microscopic image;
3) The computer controls the sample stage module (3) to move according to the magnification, so that the imaging module shoots adjacent images of the rock slice microscopic image, and the overlapping rate of the adjacent rock slice microscopic images is not lower than 20%; continuously shooting a plurality of fluorescence images, and storing the shot images in a database; the sample stage module (3) continues to move until the first rock slice completes the shooting of all positions;
4) The computer controls the sample platform module (3) to move, so that the second rock slice moves to the visual field, the steps 2) to 3) are repeated, and the like until the shooting of all positions of all rock slice samples (8) is completed;
the step S4 includes:
1) The computer numbers each of the photographed single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image;
2) The computer carries out image preprocessing on adjacent single-polarization microscopic images, then carries out image splicing, and splices the single-polarization microscopic images corresponding to the same rock slice sample (8) into a complete high-definition large image; the computer carries out image preprocessing on adjacent orthogonal polarization microscopic images, then carries out image splicing, and splices the orthogonal polarization microscopic images corresponding to the same rock slice sample (8) into a complete high-definition large image; the computer carries out image preprocessing on adjacent fluorescence microscopic images, then carries out image splicing, and splices the fluorescence microscopic images corresponding to the same rock slice sample (8) into a complete high-definition large image;
3) The computer stores the single polarization microscopic image, the orthogonal polarization microscopic image and the fluorescence microscopic image of the same rock slice sample (8) as a group in a database; when the computer calls the images of the database, the thumbnails of the high-definition large images are displayed on a computer display screen, meanwhile, a selection frame is displayed on the thumbnails, and the high-definition images at the positions selected by the selection frame are displayed in the center of the display screen; and the selection square frame is dragged, and the high-definition image displayed on the display screen moves along with the selection square frame, so that convenient observation is realized.
10. The method for digital automatic acquisition of multidimensional rock slices as recited in claim 8, wherein image stitching comprises:
image processing:
firstly, randomly selecting 10-20 pixel points in each shot image, and then calculating the intermediate value of a brightness channel of the selected pixel points; then obtaining the average value of the brightness intermediate values of all the images to be spliced;
then, averaging the brightness of all the images, namely adjusting the brightness of each image to ensure that the brightness median of each image is equal to the average value of the brightness median of all the images;
image segmentation:
each image to be spliced is divided into 10000 sub-images, and then the texture characteristics of each sub-image are calculated;
and then comparing the texture characteristics of the edge sub-images of the adjacent images to be spliced, and marking the sub-images with the same texture characteristics as the same sub-images.
Image splicing:
splicing the adjacent images to be spliced by taking the same sub-images marked in the adjacent images to be spliced as an alignment reference; and by parity of reasoning, completing the splicing of all the images to be spliced and obtaining the large-view spliced image.
CN202211122441.XA 2022-09-15 2022-09-15 Multi-dimensional rock slice digital automatic acquisition system and acquisition method Pending CN115656166A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117571733A (en) * 2023-11-21 2024-02-20 核工业北京地质研究院 Mechanical rock breaking damage characteristic testing device and testing method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117571733A (en) * 2023-11-21 2024-02-20 核工业北京地质研究院 Mechanical rock breaking damage characteristic testing device and testing method thereof

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