CN115644456A - Water-soluble heme-polypeptide composite nutrient iron fortifier and preparation method thereof - Google Patents
Water-soluble heme-polypeptide composite nutrient iron fortifier and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a water-soluble heme-polypeptide composite nutrient iron fortifier and a preparation method thereof, belonging to the field of food processing. The invention obtains the pig hemoglobin powder after the pig hemoglobin is subjected to acid hydrolysis, pH value adjustment, washing and drying precipitation, the pig hemoglobin powder is dissolved by a sodium carbonate solution and then is mixed with a whey protein solution, the pH value of the mixed solution is adjusted to be neutral, a small amount of insoluble parts are removed by centrifugation or filtration, and the water-soluble heme-polypeptide composite nutrient iron enhancer is obtained after the pig hemoglobin powder is freeze-dried or spray-dried into powder. The enhancer can well supplement iron element required by human body, the whey protein has physiological functions of regulating immunity, regulating lactose absorption and the like, and the water-soluble heme-polypeptide composite nutrient iron enhancer obtained by combining heme iron and the whey protein has high nutritional value and unique functional characteristics.
Description
Technical Field
The invention belongs to the field of food processing, and particularly relates to a water-soluble heme-polypeptide composite nutrient iron fortifier and a preparation method thereof.
Background
Domestic livestock and poultry have abundant blood resources, for example, live pigs, the national annual slaughter amount is about 6.4 hundred million, and the produced pig blood is about 300 million tons. The livestock and poultry blood dry matter is mainly protein, the content of the livestock and poultry blood dry matter accounts for 18.9 percent of the total mass of the blood, the livestock and poultry blood dry matter is an ideal protein resource, the livestock and poultry blood dry matter is mainly processed into blood powder, protein powder and the like at present and is used as animal feed, and a considerable part of blood is directly discharged as waste, so that the resources are wasted and the environment is polluted.
Blood is composed of two parts, plasma (65%) and blood cells (35%), including red blood cells, white blood cells and platelets, and mainly red blood cells. Porcine hemoglobin is the major component of porcine red blood cells, and accounts for about 95% of total red blood cell protein and 67% of total whole blood protein. The porcine hemoglobin is a tetramer which is composed of four subunits and has a certain space conformation, wherein the tetramer contains two alpha subunits and two beta subunits, each subunit is composed of a peptide chain and a heme molecule, the peptide chains are mutually coiled and folded into a sphere, and the heme molecules are wrapped in the peptide chains. Porcine hemoglobin is not only a high quality protein, but also an important source of iron nutrition.
Iron is an essential trace element of human body, and the shortage of iron is a worldwide problem. The dietary iron is divided into heme iron and non-heme iron, and the heme iron has high bioavailability and is easier to absorb than non-organic iron in plants or iron salts commonly used in food fortification. Traditional iron supplement agents such as ferrous sulfate, ferrous gluconate, ferrous fumarate and the like are inorganic iron or plant iron, and although the iron supplement effect is good, the traditional iron supplement agents have low utilization rate in vivo (the absorption rate is generally 5-8%), large side effects (symptoms such as nausea, gastrectasia, digestive organ disorder, diarrhea, constipation and the like are easy to occur), and have special metal rust taste, so that the traditional iron supplement agents are difficult to eat for a long time. The heme iron is a biological state iron, the content of iron in 100g of pig blood is up to more than 40mg, and the heme iron is absorbed through a heme transporter which is mainly expressed in a brush-shaped boundary membrane of duodenal cells and liver cells. In contrast, non-heme iron is transported into the body through non-selective iron channels, which are easily affected by other metal ions. Therefore, the absorption of heme iron by human is more complete than that of non-heme iron, and the bioavailability is high (the absorption rate reaches 15% -25%).
The effective absorption rate of heme iron is 25% on average, and 40% in the absence of iron, and in addition, heme iron absorption is also affected by food factors. Heme iron has been widely used as an iron fortifier in japan, the united states, and the like. The most common heme iron fortified foods on the japanese market are both low-calorie candies and low-calorie cookies. The heme iron reinforcer in the Chinese market is also mainly three of iron supplement tablets, iron supplement soft sweets and iron supplement capsules. The heme iron tablet, biscuit, etc. are convenient to carry, but the human absorption rate of heme iron existing in a water-insoluble manner is relatively low, and the absorption rate of heme iron in a dissolved state can be increased by 5 times. Since free heme iron is insoluble in water, most heme iron fortifiers on the market come in solid form. At present, researchers at home and abroad explore ways and methods for increasing the iron content of plant food from many aspects and obtain greater progress and efficacy, but the ways and methods for increasing the iron content of animal food are rarely researched.
The water-soluble heme-polypeptide composite nutrient iron fortifier can be added into any kind of food according to the needs, directly eaten as solution food or eaten as solid food, enters the digestive tract and is dissolved in digestive juice, and the bioavailability is obviously higher than that of insoluble heme iron. In addition, eight essential amino acids required by human body can be supplemented besides supplementing iron, and the whey protein can also supplement protein, so that the nutrient supplement is an ideal nutrient supplement.
Disclosure of Invention
The invention makes pig hemoglobin or fresh pig red blood cells undergo the process of acid hydrolysis to make heme, hydrophobic amino acid and small peptide enter into the precipitation, then the pH value is regulated to neutrality, the precipitation is washed by water, and the acid, alkali and various water-soluble components are cleaned, so as to obtain the mixture of heme, oligopeptide and amino acid, and said mixture is insoluble in water but soluble in sodium bicarbonate solution. And (3) drying the mixture, dissolving the mixture by using a sodium carbonate solution, or directly adding sodium bicarbonate to dissolve the mixture, then mixing the mixture with a whey protein aqueous solution, adjusting the pH value to be neutral, and drying the mixture to obtain the water-soluble heme iron-polypeptide composite enhancer.
The invention aims to provide a water-soluble heme-polypeptide composite nutrient iron fortifier and a preparation method thereof. The heme iron nutrition enhancer capable of being directly dissolved by water can be obtained by the method, so that iron can be supplemented to iron-deficient people more conveniently and efficiently, and meanwhile, polypeptides and proteins can be supplemented.
The purpose of the invention is realized by the following technical scheme:
a preparation method of a water-soluble heme-polypeptide composite nutrient iron fortifier comprises the following steps: hydrolyzing porcine hemoglobin with acid to obtain porcine hemoglobin, mixing the porcine hemoglobin solution and whey protein solution, adjusting pH of the mixed solution to neutral, centrifuging or filtering to remove insoluble part, and lyophilizing or spray drying to obtain powder of water soluble heme-polypeptide composite nutrient iron fortifier.
The swine heme solution is preferably obtained by dissolving swine heme powder by using a sodium carbonate solution. The concentration of the sodium carbonate solution is preferably 0.01 to 0.1%. The method for preparing the pig heme acid by hydrolysis comprises the following steps: dissolving pig hemoglobin with water, adjusting the pH value to 1.5-2.5, hydrolyzing at 80-90 ℃ for 6-12 h, adjusting the pH value to 6.0-7.5, centrifuging, washing precipitates, and freeze-drying to obtain the pig hemoglobin powder.
The concentration of the pig heme solution is preferably 0.15-0.75% (w/v).
The concentration of the whey protein solution is preferably 1.5 to 6.0% (w/v).
In the mixed solution, the concentration of the whey protein is preferably 0.5-2.0% (w/v), and the concentration of the porcine heme is preferably 0.1-0.5% (w/v).
The above pH adjustment is preferably carried out using HCl solution and NaOH solution.
The preparation method of the water-soluble heme-polypeptide composite nutrient iron fortifier comprises the steps of adding whey protein to form a nano-composite with heme, and after the nano-composite is adjusted to be neutral, the heme can stably exist. The water-soluble heme-polypeptide composite nutrient iron fortifier is prepared by dissolving the water-soluble heme-polypeptide composite nutrient iron fortifier with water after being sprayed or freeze-dried.
A water-soluble heme-polypeptide composite nutrient iron fortifier is obtained by the preparation method. The enhancer can well supplement iron element required by human body, and the whey protein has physiological functions of regulating immunity, regulating lactose absorption, etc. Whey protein is called active protein in milk, and the active protein plays an important role in metabolism and nutritional health of human bodies. The combination of heme iron and whey protein has high nutritive value and unique functional characteristics.
The invention has the following advantages:
(1) The biological utilization rate of the heme iron is high, and the problem of iron deficiency of organisms is solved;
(2) Can be added into food in any form to serve as high-efficiency iron supplement food;
(3) Easy to dissolve in water, convenient to use and high in bioavailability;
(4) Can be used as iron enhancer and polypeptide/amino acid enhancer;
(5) The important production raw material of the pig hemoglobin is cheap and easy to obtain and has rich sources;
(6) The product has good stability and low hygroscopicity in a high-humidity environment;
(7) The production is simple and easy, and the practicability is strong.
Drawings
FIG. 1 shows the degree of hydrolysis of porcine hemoglobin at various times.
FIG. 2 is an SDS-PAGE image of porcine hemoglobin-hydrolyzed supernatant.
FIG. 3 is a UV-Vis spectrum of a porcine hemoglobin hydrolysis precipitate.
FIG. 4 is a chromatogram of hemoglobin. Wherein (A) a heme standard; (B) hydrolyzing heme in the precipitate for 6 h; and (C) hydrolyzing the heme in the precipitate for 12 hours.
FIG. 5 is a hemoglobin quantification standard curve.
FIG. 6 is the mean particle size of the whey protein-porcine heme complexes at different heme concentrations. In the figure, the concentration of whey protein is 1%, and the concentration of the pig heme powder is 0%, 0.1%, 0.3% and 0.5% in sequence.
Fig. 7 is the appearance characteristics of whey protein-heme nanocomposite of different pig heme powder concentrations, (a) aqueous solution, (B) spray drying. In the figure, the concentration of whey protein is 1%, and the concentration of the pig heme powder is 0%, 0.1% and 0.3% in sequence.
Fig. 8 is a microscopic view of whey protein-heme nano-complexes at different concentrations of porcine heme powder. In the figure, the concentration of whey protein is 1%, and the concentration of the pig heme powder is 0% (A), 0.1% (B), 0.3% (C) and 0.5% (D) in sequence.
Figure 9 is the resolubility of whey protein-heme nanocomposite for different pig heme powder concentrations. FIG. A is an appearance diagram, and FIG. B is a measurement of solubility. Wherein the whey protein concentration is 1%, and the pig heme powder concentration is 0.1%, 0.3%, 0.5%.
Figure 10 is the hygroscopicity of whey protein/heme complexes at different concentrations of porcine heme powder. Wherein the concentration of Whey Protein (WP) is 1%, the concentration of pig Heme powder (Heme) is 0.1%, 0.3%, 0.5%, and the moisture absorption rate of the compound is measured at 25 deg.C and 75% humidity respectively.
Detailed Description
The invention provides a preparation method of a water-soluble heme-polypeptide composite nutrient iron fortifier, which can be directly dissolved by water.
1. The preparation method of the water-soluble heme-polypeptide composite nutrient iron fortifier by using fresh pig blood and whey protein powder as raw materials comprises the following steps:
(1) 2.5L of fresh pig blood is taken, added with 3.0% (w/v) of trisodium citrate (anticoagulant) 250mL of solution, centrifuged at 3000rpm for 10min, and the precipitate is collected and the supernatant is discarded.
(2) The collected pig blood red cells were washed 3 times with 0.9% (w/v) physiological saline and centrifuged at 3000rpm for 10min each time.
(3) To the pig blood erythrocytes collected after washing, 2 times the volume of pure water was added, and the mixture was gently stirred for 20min. And centrifuging the crushed solution at 10000rpm for 20min. Collecting supernatant, discarding the precipitate, collecting the supernatant as pig hemoglobin stock solution, and lyophilizing to obtain pig hemoglobin powder.
(4) Dissolving the pig hemoglobin powder with deionized water to obtain pig hemoglobin solution with concentration of 2% (w/v), stirring at room temperature until completely dissolved, centrifuging at 8000rpm for 10min, and discarding the precipitate to obtain supernatant.
(5) The pH of the 2% pig hemoglobin solution was adjusted to 2.0 with 6mol/L HCl solution. Heating and hydrolyzing in a water bath at 80 ℃ for 12h, adjusting the pH to 7.0 by using 2mol/L NaOH solution, centrifuging at 8000rpm for 20min, and removing the supernatant to obtain a precipitate. Washing the precipitate with deionized water, centrifuging at 8000rpm for 10min, and repeating for 3 times. Freeze-drying the precipitate for 24h, and grinding into powder to obtain the pig heme powder.
(6) Dissolving the pig heme powder in 0.1% sodium carbonate solution to prepare 0.15-0.75% (w/v) heme solution, stirring at room temperature in a dark place until the heme solution is completely dissolved, centrifuging at 8000rpm for 10min, and removing the precipitate to leave supernatant. Dissolving whey protein powder with deionized water to obtain 3% (w/v) whey protein solution, stirring at room temperature to completely dissolve, and centrifuging at 8000rpm for 10min. The precipitate was discarded and the supernatant was obtained.
(7) Mixing the pig heme solution and the whey protein solution in a volume ratio of 1. Wherein the final concentration of the whey protein is 1 percent, and the final concentration of the pig heme powder is 0.1 to 0.5 percent.
(8) The whey protein-heme compound solution is freeze-dried or spray-dried to prepare the water-soluble heme-polypeptide compound nutrient iron fortifier.
2. The method for preparing the water-soluble heme-polypeptide composite nutrient iron fortifier by taking commercially available hemoglobin powder as a raw material specifically comprises the following steps:
(1) Dissolving the pig hemoglobin powder with deionized water to obtain 2% pig hemoglobin solution, stirring at room temperature until completely dissolved, centrifuging at 8000rpm for 10min, and discarding the precipitate to obtain supernatant.
(2) The pH of the 2% pig hemoglobin solution was adjusted to 2.0 with 6mol/L HCl solution. Heating in a water bath at 80 deg.C for hydrolysis for 12h, adjusting pH to 7.0 with 2mol/L NaOH solution, centrifuging at 8000rpm for 20min, and discarding the supernatant to obtain precipitate. The precipitate was washed with deionized water and centrifuged at 8000rpm for 10min, which was repeated 3 times. Freeze drying the precipitate for 24 hr, and grinding into powder to obtain heme powder.
(3) Dissolving the pig heme powder in 0.1% sodium carbonate solution to prepare 0.15-0.75% heme solution, stirring at room temperature in a dark place until the heme solution is completely dissolved, centrifuging at 8000rpm for 10min, and discarding the precipitate to leave the supernatant. The whey protein powder is dissolved by deionized water to prepare a 3 percent whey protein solution, and is stirred at room temperature until the whey protein solution is completely dissolved, and then is centrifuged at 8000rpm for 10min. The precipitate was discarded and the supernatant was obtained.
(4) Mixing the pig heme solution and the whey protein solution in a volume ratio of 1. Wherein the final concentration of the whey protein is 1 percent, and the final concentration of the pig heme powder is 0.1 to 0.5 percent.
(5) The whey protein-heme compound solution is freeze-dried or spray-dried to prepare the water-soluble heme-polypeptide compound nutrient iron fortifier.
The advantages and effects of the present invention will be further described with reference to specific examples.
Example 1
(1) Commercially available porcine hemoglobin powder was dissolved sufficiently at a concentration of 2%, centrifuged at 8000rpm for 10min to remove insoluble fraction, and the precipitate was discarded to leave a supernatant.
(2) The pH of the 2% pig hemoglobin solution was adjusted to 2.0 using 6mol/L HCl solution.
(3) Heating the porcine hemoglobin solution with pH of 2.0 at 80 deg.C for 12h, and sampling every 2 h.
(4) The extracted pig hemoglobin solution is cooled, the pH value is adjusted to 7.0 by 2 mol/L/NaOH, and the solution is centrifuged for 20min at 8000 rpm. Collecting supernatant, measuring the hydrolysis degree, washing the precipitate with water, centrifuging at 8000rpm for 20min, repeating for three times, discarding the supernatant, and retaining the precipitate.
(5) And determining the hydrolysis degree, the amino acid type and the heme content by using an ultraviolet spectrophotometer, SDS-PAGE electrophoresis, an amino acid analyzer and high performance liquid chromatography. As shown in fig. 1, the hydrolysis value reached a maximum after 12h of hydrolysis; after 12h of hydrolysis, as shown in FIG. 2, the protein was hydrolyzed to amino acids, and the band disappeared; 16 amino acids after 12h hydrolysis of pig hemoglobin were measured as shown in table 1; as shown in FIG. 3, the ultraviolet absorption intensity of heme at 383nm is stronger after hydrolysis for 12 h; the peak time of the hemoglobin liquid phase after 12h hydrolysis is consistent with the hemoglobin standard as shown in FIG. 4. As shown in Table 2, the hemoglobin content was higher after 12h hydrolysis according to the standard curve of hemoglobin standard (FIG. 5).
TABLE 1 hydrolyzed amino acid content in porcine hemoglobin hydrolyzed precipitate
TABLE 2 hydrolyzed amino acid content in porcine hemoglobin hydrolysis precipitate
(6) And (4) freeze-drying the precipitate washed in the step (4) to obtain the powder of the pig heme.
Example 2
(1) Dissolving pig heme powder with 0.1% sodium carbonate solution at concentrations of 0.15%, 0.45% and 0.75%, centrifuging at 8000rpm for 10min, removing insoluble part, and collecting supernatant and removing precipitate to obtain pig heme solution.
(2) Fully dissolving the whey protein powder with deionized water according to the concentration of 3%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, discarding precipitate, and preparing into whey protein solution.
(3) The pig heme solution and the whey protein solution are mixed together in a volume ratio of 2.
(6) The particle size of the three whey protein-heme complex solutions was measured using a particle sizer, as shown in fig. 6, the particle size in the complex solution increased with the increase of heme concentration, and the particle size was maximized when the pig heme powder concentration reached 0.5% (w/v).
Example 3
(1) Dissolving pig heme powder with 0.1% sodium carbonate solution at concentrations of 0.15%, 0.45%, and 0.75%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, and removing precipitate to obtain pig heme solution.
(2) Fully dissolving the whey protein powder with deionized water according to the concentration of 3%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, discarding precipitate, and preparing into whey protein solution.
(3) The pig heme solution and the whey protein solution are mixed together in a volume ratio of 2. The appearance was observed by taking out (FIG. 7A), and it was found that the color of the solution became darker as the concentration of hemoglobin increased.
(4) And (4) sucking the solution obtained in the step (3) into a spray dryer for drying, setting the temperature of an air inlet of the spray dryer to be 140 ℃, the rotating speed of a peristaltic pump to be 15rpm, the frequency of a fan to be 60Hz and the temperature of an air outlet to be 70 ℃, and collecting powder. The appearance was observed by removing (FIG. 7B), and it was found that the powder became darker as the hemoglobin concentration increased.
Example 4
(1) Dissolving pig heme powder with 0.1% sodium carbonate solution at concentrations of 0.15%, 0.45% and 0.75%, centrifuging at 8000rpm for 10min, removing insoluble part, and collecting supernatant and removing precipitate to obtain pig heme solution.
(2) Fully dissolving whey protein powder with deionized water according to the concentration of 3%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, discarding precipitate, and preparing into whey protein solution.
(3) The porcine heme solution and the whey protein solution were mixed together at a volume ratio of 2.
(4) And (4) quickly freezing the supernatant in the step (3) by using liquid nitrogen, putting the frozen supernatant into a freeze dryer, drying the frozen supernatant for 24 hours to form powder, and observing the microstructure of the powder.
(5) As shown in fig. 8, whey protein is spherical nanoparticles, and becomes irregular-shaped nanoparticles after hemoglobin is added, and the particles are largest when the hemoglobin concentration is the largest.
Example 5
(1) Dissolving pig heme powder with 0.1% sodium carbonate solution at concentrations of 0.15%, 0.45%, and 0.75%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, and removing precipitate to obtain pig heme solution.
(2) Fully dissolving the whey protein powder with deionized water according to the concentration of 3%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, discarding precipitate, and preparing into whey protein solution.
(3) The pig heme solution and the whey protein solution are mixed together in a volume ratio of 2.
(4) And (4) sucking the solution obtained in the step (3) into a spray dryer for drying, setting the temperature of an air inlet of the spray dryer to be 140 ℃, the rotating speed of a peristaltic pump to be 15rpm, the frequency of a fan to be 60Hz and the temperature of an air outlet to be 70 ℃, and collecting powder.
(5) The whey protein-heme powder of 3 different concentrations was dissolved with deionized water, and no precipitate was found after centrifugation at 8000rpm for 10min, and the solubility reached 98%, the results are shown in fig. 9.
Example 6
(1) Dissolving pig heme powder with 0.1% sodium carbonate solution at concentrations of 0.15%, 0.45%, and 0.75%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, and removing precipitate to obtain pig heme solution.
(2) Fully dissolving whey protein powder with deionized water according to the concentration of 3%, centrifuging at 8000rpm for 10min, removing insoluble part, retaining supernatant, discarding precipitate, and preparing into whey protein solution.
(3) The pig heme solution and the whey protein solution are mixed together in a volume ratio of 2.
(4) And (4) sucking the solution obtained in the step (3) into a spray dryer for drying, setting the temperature of an air inlet of the spray dryer to be 140 ℃, the rotating speed of a peristaltic pump to be 15rpm, the frequency of a fan to be 60Hz and the temperature of an air outlet to be 70 ℃, and collecting powder.
(5) The different samples were placed in a desiccator saturated with NaCl solution at 25 ℃ and 75% humidity. The mass was measured at different time points (2, 4, 6, 8, 10, 12, 24, 48, 60, 72, 84, 96, 108, 120h.. 216 h) and the moisture pick-up was calculated. As shown in FIG. 10, the results indicated that the moisture absorption rate was lowest at a hemoglobin concentration of 0.5%.
It should be understood that the present invention is not limited to the above-described embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and they are intended to be included in the scope of the present invention.
Claims (10)
1. A preparation method of a water-soluble heme-polypeptide composite nutrient iron fortifier is characterized by comprising the following steps: the method comprises the following steps: hydrolyzing porcine hemoglobin with acid to obtain porcine hemoglobin, mixing the porcine hemoglobin solution and whey protein solution, adjusting pH of the mixed solution to neutral or near neutral, centrifuging or filtering to remove insoluble part, and lyophilizing or spray drying to obtain powder of water soluble heme-polypeptide composite iron supplement.
2. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: the pig heme solution is obtained by dissolving pig heme in a sodium carbonate solution.
3. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 2, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: the concentration of the sodium carbonate solution is 0.01-0.1%.
4. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 2, wherein: the method for preparing the porcine heme acid hydrolysis comprises the following steps: dissolving pig hemoglobin with water, adjusting the pH value to 1.5-2.5, hydrolyzing at 80-90 ℃ for 6-12 h, adjusting the pH value to 6.0-7.5, centrifuging, washing precipitates, and freeze-drying to obtain the pig hemoglobin powder.
5. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: the pH was adjusted using HCl solution and NaOH solution.
6. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: the concentration of the pig heme solution is 0.15 to 0.75 percent.
7. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: the concentration of the whey protein solution is 1.5-6.0%.
8. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein the water-soluble heme-polypeptide complex nutrient iron fortifier comprises the following steps: in the mixed solution, the concentration of whey protein is 0.5-2.0%, and the concentration of the pig heme is 0.1-0.5%.
9. The method for preparing a water-soluble heme-polypeptide complex nutrient iron fortifier according to claim 1, wherein: the pH was adjusted using HCl solution.
10. A water-soluble heme-polypeptide composite nutrient iron fortifier is characterized in that: obtained by the production method according to any one of claims 1 to 9.
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| US4518525A (en) * | 1982-03-12 | 1985-05-21 | Valtion Teknillinen Tutkimuskeskus | Method for dividing blood hemoglobin into heme and globin |
| US6217932B1 (en) * | 1996-12-20 | 2001-04-17 | Fraunhofer-Gesell Schaft Zur Forderung Der Angewandten Forschung E.V. | Method of obtaining haemin from slaughter blood |
| CN1572153A (en) * | 2003-06-04 | 2005-02-02 | 营养学总公司 | Method for obtaining a food product containing heme iron and food product thus obtained |
| CN101748181A (en) * | 2010-01-15 | 2010-06-23 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
| CN102401813A (en) * | 2010-09-17 | 2012-04-04 | 国立交通大学 | Rapid analysis method for hemolglobin variant |
| JP2014113063A (en) * | 2012-12-06 | 2014-06-26 | Univ Of Miyazaki | Method for manufacturing composite nanoparticles including heme iron having high water dispersibility and milk protein |
| CN114601170A (en) * | 2022-02-22 | 2022-06-10 | 湖北工业大学 | New method for dissolving heme |
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4518525A (en) * | 1982-03-12 | 1985-05-21 | Valtion Teknillinen Tutkimuskeskus | Method for dividing blood hemoglobin into heme and globin |
| US6217932B1 (en) * | 1996-12-20 | 2001-04-17 | Fraunhofer-Gesell Schaft Zur Forderung Der Angewandten Forschung E.V. | Method of obtaining haemin from slaughter blood |
| CN1572153A (en) * | 2003-06-04 | 2005-02-02 | 营养学总公司 | Method for obtaining a food product containing heme iron and food product thus obtained |
| CN101748181A (en) * | 2010-01-15 | 2010-06-23 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
| CN102401813A (en) * | 2010-09-17 | 2012-04-04 | 国立交通大学 | Rapid analysis method for hemolglobin variant |
| JP2014113063A (en) * | 2012-12-06 | 2014-06-26 | Univ Of Miyazaki | Method for manufacturing composite nanoparticles including heme iron having high water dispersibility and milk protein |
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