CN115644195A - Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof - Google Patents
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a quick-acting traditional Chinese medicine disinfection composition capable of being used in human space and a preparation method thereof, belonging to the technical field of traditional Chinese medicine disinfection and comprising the following raw materials in percentage by weight: 0.5-3.0% of hawthorn seed extract, 10-90% of salix leaf hydroalcoholic extract, 0.1-3% of food preservative, 0.1-1.0% of carvacrol and the balance of water. The traditional Chinese medicine disinfection composition has the effect of 30s, the killing log value of escherichia coli, staphylococcus aureus and staphylococcus albus is more than 5.00, the killing log value of candida albicans is more than 4.00, the natural fragrance of willow leaves is realized, the traditional Chinese medicine disinfection composition is nontoxic, the traditional Chinese medicine disinfection composition can be used in human environments such as families, hospitals, offices, subways, high-speed rails, buses and the like in a fumigating and spraying mode, and the traditional Chinese medicine disinfection composition has important practical significance for preventing respiratory infectious diseases such as new coronary pneumonia, influenza, tuberculosis, legionella pneumophila and the like and restoring normal social order in the era after new coronary epidemic situation.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine disinfection, and particularly relates to a quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and a preparation method thereof.
Background
Infectious diseases cause unprecedented harm and loss to the social economic order of the world and the life and health of people, and like air disinfection of public environments in which people such as airplanes, high-speed rails, subways, markets, factories, hospitals, schools, kindergartens and the like are relatively gathered becomes a key point, however, the disinfectants commonly used at home and abroad at present are chemical preparations such as peracetic acid, hydrogen peroxide, chlorine dioxide, glutaraldehyde, iodine, quaternary ammonium salt, formaldehyde and 84 disinfectant, although the chemical preparation disinfectants can effectively kill pathogenic microorganisms, but generally have toxic and side effects, irritation and corrosiveness, are not suitable for or can not be used in human environments, and have adverse effects on ecological environments and human health after long-term use, so the air disinfectants with high safety performance and used in human environments are especially lacking in the prior art.
Infectious diseases caused by pathogenic bacteria transmitted through the air belong to the category of warm diseases in traditional Chinese medicine, and the 'Huangdi' book of more than two thousand years ago in China has records for preventing infectious diseases; the 'hou guan fang' of jin zhang has recorded "alu sore" (smallpox) and "rabies" (rabies), and the latter books have clearly recorded malaria, measles, diphtheria, chicken pox, cholera, dysentery, tuberculosis and other acute infectious diseases and the treatment methods based on syndrome differentiation; according to the research progress of the air disinfection method of traditional Chinese medicine in the 2 nd stage of volume 3 of 2003, 4 months of 2003, the traditional Chinese medicines for air disinfection mainly have volatility, heat and toxic materials clearing, and dampness eliminating effects, and comprise rhizoma Atractylodis, radix Isatidis, herba Houttuyniae, radix et rhizoma Rhei, coptidis rhizoma, cortex Phellodendri, herba Agastaches, flos Caryophylli, herba asari, folium Salicis Babylonicae, semen crataegi, folium Artemisiae Argyi, radix Et rhizoma Fagopyri Tatarici, flos Sophorae Immaturus, fructus Cnidii, herba Hedyotidis Diffusae, fructus Zanthoxyli, herba Menthae, radix Sophorae Flavescentis, radix Stemonae, borneolum Syntheticum, mentholum, flos Lonicerae, herba Taraxaci, fructus Gardeniae, glycyrrhrizae radix, radix Ophiopogonis, etc.; in the last decade, the research or literature on the use of volatile plant (Chinese medicine) essential oil as an air fumigation disinfectant is increasing, including clove oil, thymol, forsythia oil, wormwood oil, tea tree oil, patchouli oil, etc.
The hawthorn seeds as traditional Chinese medicines are already recorded in the 2020 edition pharmacopoeia of China, the hawthorn seed extract can be applied to disinfection and sterilization, for example, 3 months and 31 days in 2022, shanxi province Weijian Commission approved Shanxi Baimutang science and technology Limited company uses the hawthorn seed extract to produce the Jingkun R hawthorn seed gynecological antibacterial gel (the second type disinfection product), but because the concentration required by using hawthorn seed essence for disinfection is higher (the general content can reach the disinfection standard in a 30-50 percent way), and the use of high concentration causes large pungent smell, the application of the hawthorn seed extract in indoor manned environment is limited.
The willow leaf is Salix babylonica (Salix babylonica) of SalicaceaeSalix babylonica L.) The Chinese patent publication No. CN 10739817A discloses a willow leaf extract, a preparation method and application thereof, the willow leaf extract adopts water and alcohol to mix and extract willow leaf effective substances, and is mainly used for lowering blood pressure and health care products, and the willow leaf extract is not used for a disinfectant; the invention publication number of Chinese patent is CN104094979A, which discloses the application of willow leaf in the preparation of disinfectants, and although the patent describes that willow leaf extract can be used for air disinfection, no experiment for screening and identifying neutralizing agent according to the disinfection effect evaluation method of 2002 edition 'disinfection technical Specification' is shown, so the disinfection effect of the willow leaf extract is unknown.
Therefore, a quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and a preparation method thereof are needed.
Disclosure of Invention
Aiming at the situation and overcoming the defects of the prior art, the invention provides a quick-acting traditional Chinese medicine disinfection composition capable of being used in a human space and a preparation method thereof, aiming at solving the problem that the existing disinfectant is difficult to be used in the human space, the invention adopts hawthorn seed essence, salix leaf extract and carvacrol as main active components for disinfection, thereby reducing the damage of the disinfectant to human body, simultaneously, the invention obtains the colorless hawthorn seed essence without pungent smell through re-refluxing and separation, solves the technical problem that the hawthorn seed essence is difficult to be used as an air type disinfectant in the prior art, and solves the problem that the salix leaf extract is easy to oxidize and discolor by adding an antioxidant, in addition, the invention adopts silicon dioxide aerogel to adsorb the carvacrol and a food preservative as a synergistic agent of the salix leaf extract, thereby obviously improving the disinfection effect of the salix leaf extract, the invention strictly follows the disinfection effect evaluation method of the disinfection technical specification (2002 edition), and carries out neutralizer screening test and disinfection effect identification test, and proves that the composition has the killing logarithm value of more than 5.00 on escherichia coli, staphylococcus aureus and staphylococcus albus, the killing logarithm value of more than 4.00 on candida albicans, the killing logarithm value of more than 4.00 on coronavirus and quick disinfection time and multiple types, meets the standard requirements of the disinfection technical specification (2002 edition) The environment and air of people in hospitals, sanitary places and the like are disinfected.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: the invention provides a quick-acting traditional Chinese medicine disinfection composition capable of being used in human space and a preparation method thereof, and the quick-acting traditional Chinese medicine disinfection composition is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials: fructus crataegi kernel essence, salix leaf hydroalcoholic extract, carvacrol, food preservative and water.
Further, the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight: 0.5-3.0% of hawthorn seed extract; 10-90% of salix leaf hydroalcoholic extract; carvacrol 0.1-1.0%; 0.2-1.0% of food preservative; the balance of water.
As a further preferred aspect of the present invention, the Chinese medicinal disinfectant composition comprises the following raw materials by weight: 0.5% of hawthorn kernel extract; salix leaf hydroalcoholic extract 90.0%; carvacrol 0.2%; 0.3% of food preservative; 9.0 percent of water.
As a further preferred aspect of the present invention, the Chinese medicinal disinfecting composition comprises the following raw materials by weight: 0.8% of hawthorn kernel extract; 10.0% of salix leaf hydroalcoholic extract; 1.0% of carvacrol; 0.2% of food preservative; and (5) 88.0 percent of water.
As a further preferred aspect of the present invention, the Chinese medicinal disinfecting composition comprises the following raw materials by weight: 1% of hawthorn seed extract; salix leaf hydroalcoholic extract 80%; carvacrol 0.1%; 0.5% of food preservative; and (4) 18.4% of water.
As a further preferred aspect of the present invention, the Chinese medicinal disinfecting composition comprises the following raw materials by weight: 2% of hawthorn seed extract; salix leaf hydroalcoholic extract 90%; carvacrol 0.2%; 0.3% of food preservative; and 7.5% of water.
As a further preferred aspect of the present invention, the Chinese medicinal disinfecting composition comprises the following raw materials by weight: 3% of hawthorn seed extract; 10% of salix leaf hydroalcoholic extract; 1.0% of carvacrol; 0.2% of food preservative; and 85.8 percent of water.
Further, the traditional Chinese medicine disinfection composition comprises the application of any one of the following components:
(1) Applied to surface disinfection, including skin surfaces, object surfaces, melon, fruit and vegetable surfaces;
(2) The composition is applied to killing one or more of escherichia coli, staphylococcus aureus, staphylococcus albus, influenza A virus and coronavirus;
(3) The product can be used for preventing respiratory infectious diseases, including new coronary pneumonia, influenza, tuberculosis, and legionella pneumophila.
In addition, the invention also provides a preparation method of the quick-acting traditional Chinese medicine disinfection composition capable of being used in the space with people, which is characterized by comprising the following steps: the preparation method of the traditional Chinese medicine disinfection composition comprises the following steps:
(1) Preparation of the hawthorn seed extract
Mixing the raw material of the hawthorn seed powder with distilled water according to the proportion of 1-4, and distilling for 1-3 times under reduced pressure at 40-65 ℃ to obtain colorless and transparent distillate or dark brown concentrate, wherein the pH value of the distillate is 2.0-3.0, and the organic acid content of the distillate is 4-9%;
(2) Preparation and antioxidation of the salix leaf hydroalcoholic extract
Picking leaves of Salix babylonica, naturally airing or sun drying, soaking in 15-20 times volume of ethanol-water solution containing 5-10% (v/v) ethanol for 24 hr, reflux extracting at 90 deg.C for 2 hr, squeezing, filtering, and collecting filtrate; adding 10 times of water into the filter residue, continuously extracting for 1h under reflux at 90 ℃, squeezing and filtering, collecting the filtrate, and combining the filtrates obtained by two-time extraction to obtain saligenin crude extract; the pH value of the salix leaf hydroalcoholic extract is 4.5-5.5; immediately adding 0.1% (v/v) citric acid and 0.1% (v/v) ascorbic acid to ensure that the willow leaf extract is not oxidized and discolored and the appearance can always keep clear light tea color, if citric acid and ascorbic acid are not added, the willow leaf extract begins to discolor after being placed for 5 minutes and turns into dark brown after 1 hour, thus directly influencing the appearance and the use; stirring until citric acid and ascorbic acid are completely dissolved, cooling in water bath, sealing, and storing in dark place;
(3) Preparation of the carvacrol
Mixing and dissolving a carvacrol raw material and absolute ethyl alcohol according to a volume ratio of 1; the silicon dioxide aerogel is a nano material with a three-dimensional network structure, has a particle size of 100 nm and a spatial structure of less than 100 nm, has a thickness of 10000 nanopores per millimeter, can achieve high drug loading rate, can adsorb and form a nano carvacrol dispersoid which is not easy to agglomerate in a self-assembly mode, can replace a solvent method to dissolve carvacrol, and is added into the aqueous solution of the traditional Chinese medicine disinfection composition;
(4) Preparation of the Chinese medicinal disinfectant composition
Mixing the hawthorn seed essence prepared in the step (1), the salix leaf hydroalcoholic extract prepared in the step (2), the carvacrol prepared in the step (3), the food preservative and water according to a ratio, and homogenizing and stirring to obtain the traditional Chinese medicine disinfection composition, wherein the dosage form of the traditional Chinese medicine disinfection composition comprises aqua, gel, solid and powder.
Preferably, in the step (1), the raw materials of the hawthorn-kernel extract and the distilled water are mixed according to the proportion of 1.
Preferably, in the step (2), the willow leaves are naturally dried, soaked in 20 times of ethanol-water solution for 24 hours, the ethanol content in the water solution is 5%, and the willow leaf hydroalcoholic extract which is oxidation-resistant and does not discolor is prepared by extraction.
Preferably, the content of carvacrol in the step (3) is 0.1% by weight, specifically, carvacrol adsorbed by nano silica aerogel is used, carvacrol is a liquid dissolved by absolute ethyl alcohol, the ratio of ethyl alcohol to carvacrol is 1.
As a further preferred aspect of the present invention, the food preservative in step (4) is ethyl p-hydroxybenzoate, another name: ethylparaben, used in an amount of 0.25%.
The traditional Chinese medicine disinfection composition has the structure-activity relationship and the functions as follows:
the used hawthorn seed extract is the hawthorn seed extract separation liquid treated by a re-reflux process, is colorless in appearance and has no pungent smell, and the hawthorn seed extract raw material which is not subjected to reflux separation is colored and has large pungent smell, so that the hawthorn seed extract separation liquid is not beneficial to disinfecting air in the indoor environment by people;
the hawthorn seed essence in the composition has the functions of sterilizing and disinfecting the salix leaf extract, and has the function of mutual synergism when being used together with the salix leaf extract;
the antioxidant folium Salicis Babylonicae extract can reduce the usage amount and pungent smell of fructus crataegi extract;
the carvacrol has the functions of synergism and dosage reduction on the willow leaf extract and the hawthorn seed essence, and the method for adsorbing the carvacrol by the silicon dioxide aerogel can replace a solvent method;
the food preservative ethyl p-hydroxybenzoate (also called ethylparaben) has the dual effects of preserving and guaranteeing the quality of the traditional Chinese medicine disinfection composition, and has the effects of inactivating and synergizing coronavirus.
Method for identifying disinfection effect of traditional Chinese medicine disinfection composition
The invention relates to a traditional Chinese medicine disinfection composition capable of being used in human environment, which strictly follows the operating procedures of a disinfection agent identification test of 'disinfection technical Specification' 2.1.1.5.1 and a neutralizer primary selection test of 2002 edition of the ministry of health of China and a suspension quantitative identification test of a neutralizer of 2.1.1.5.5, and is determined to be a neutralizer used for a disinfection effect test of the traditional Chinese medicine disinfection composition through a neutralizer screening test, wherein the neutralizer used for the disinfection effect test of the traditional Chinese medicine disinfection composition is as follows: 3.9% D/E broth.
The invention has the following beneficial effects:
(1) The traditional Chinese medicine disinfection composition has rich and cheap raw material sources, wherein the hawthorn seeds are waste for producing fruits; willow leaves are naturally-detached wastes, and the two raw materials have high cognition degree in civilian world, including edible and medicinal use and safety, are nearly well known, and have the characteristics of low cost, simple process, convenient use, easy popularization and popularization;
(2) The purchased raw material of the hawthorn seed extract is colorless after being treated by the reflux and separation processes, so that the pungent smell is obviously reduced, and the use of the air disinfectant is facilitated;
(3) The invention obviously improves the disinfection effect through the synergistic interaction of the carvacrol and the food preservative and the combined application of the carvacrol and the willow leaf extract;
(4) The silicon dioxide aerogel is adopted for adsorbing carvacrol, and the carvacrol can be dissolved in the aqueous solution of the composition without a solvent and an emulsifier, so that the synergistic effect is achieved;
(5) The food preservative is used, so that the product can be prevented from being rotten and deteriorated, and the food preservative has the synergistic antiviral effect;
(6) The disinfection composition has the peculiar fragrance of willow leaves and the function of refreshing air, the disinfection effect is similar to that of peracetic acid or 75% alcohol, and the disinfection composition has no flammable risk, and can be used for disinfecting the environment and the air of people in families, hospitals, sanitary places and the like;
(7) The traditional Chinese medicine disinfection composition is obtained by a neutralizer required by the identification of the traditional Chinese medicine disinfection composition according to a neutralizer screening method in disinfection technical Specification (2002 edition), and the identification and killing test of the neutralizer confirms that the traditional Chinese medicine disinfection composition has credible disinfection effect and effect on escherichia coli, staphylococcus aureus, staphylococcus albus and coronavirus, and the disinfection effect time is only 30 seconds.
Drawings
FIG. 1 is a comparison of the apparent color change of willow leaf extract with and without citric acid and ascorbic acid.
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition, any methods and materials similar or equivalent to those described herein can be used in the practice of the present invention. The preferred embodiments and materials described herein are exemplary only, and are not intended to limit the scope of the present application.
The experimental methods in the following examples are all conventional methods unless otherwise specified; the test materials and test strains used in the following examples were obtained from commercial sources unless otherwise specified.
Example 1
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof
The traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight (100 kg grade): 1% of hawthorn seed extract; salix leaf hydroalcoholic extract 80%; carvacrol 0.1%; 0.5% of food preservative; and (4) 18.4% of water.
The preparation method of the traditional Chinese medicine disinfection composition comprises the following specific steps:
step 1: re-refluxing/separating and refining of haw kernel extract
The preparation method is carried out according to the patent application No. 2022116503823, specifically, a proper amount of raw material of the hawthorn kernel extract which is produced by the company chengdoubao is weighed, the organic acid content (calculated by acetic acid) is measured to be 13.48%, the phenol content (calculated by guaiacol) is 2.62mg/g, the aldehyde content (calculated by heptaldehyde) is measured to be 9.0g/100mL, the pH value is 1.84, then, 1kg of the hawthorn kernel extract is weighed and mixed with distilled water according to the mass ratio of 1, the mixture is distilled under reduced pressure for 1 time at 55 ℃, 1kg of distillate which is colorless and transparent in appearance is obtained, the measured pH value is 2.13, and the organic acid content is 4.35%, namely the hawthorn kernel extract distillate (colorless) used by the invention is obtained and is reserved.
In addition, the deep brown hawthorn kernel extract obtained in the reflux separation process is a concentrate, the pH value is 1.93, the organic acid content is 9.70%, and the deep brown hawthorn kernel extract can be used as a standby raw material of the invention to replace colorless transparent distillate, and the reference dosage is 0.25-0.5%.
Step 2: preparation of antioxidant willow leaf extract
Picking fresh willow leaves (willow leaves), naturally airing (drying in the sun) to dry, weighing 3kg of dried willow leaves, soaking for 24 hours by using 60kg of ethanol-water solution, wherein the ethanol content is 5%, extracting the willow leaves under reflux at 90 ℃ for 2 hours after soaking for 24 hours, squeezing and filtering, and collecting about 54kg of filtrate; adding the filter residue into 30kg of ethanol-water solution, continuously performing reflux extraction for 1 hour at 90 ℃, pressing and filtering, collecting 30kg of filtrate, combining 84kg of filtrate obtained by two-time extraction to obtain the salix leaf hydroalcoholic extract used by the invention, immediately adding 0.1% citric acid and 0.1% ascorbic acid into the salix leaf extract after the extraction is finished, stirring until the mixture is dissolved, sealing and storing in the dark after water bath cooling, wherein the salix leaf extract is not oxidized and does not change color, the appearance always keeps clear light tea color, the measured pH value is 5.32, the smell is aromatic, and weighing 80kg of salix leaf hydroalcoholic extract for later use.
As shown in fig. 1, the color change of the appearance of the willow leaf extract is compared with the color change of the willow leaf extract without citric acid and ascorbic acid, which is shown in detail in a and B, wherein, a is shown as follows: adding citric acid and ascorbic acid to willow leaf extract, and making it light; and B, drawing: the willow leaf extract without citric acid and ascorbic acid is similar to concentrated tea water in color.
And step 3: preparation of silica aerogel substituted solvent/adsorbed carvacrol
Weighing 1kg of commercially available silica aerogel, measuring the particle size of 80 nm, keeping for later use, weighing 1kg of commercially available carvacrol commodity, then preparing 1kg of absolute ethyl alcohol, mixing the absolute ethyl alcohol and the carvacrol according to a ratio of 1.
And 4, step 4: preparation of the traditional Chinese medicine disinfection composition
Weighing 1% (1.0 kg) of the hawthorn kernel refined reflux liquid (colorless isolate) obtained in the steps 1, 2 and 3, the salix leaf hydroalcoholic mixed extract, carvacrol adsorbed by silicon dioxide, a food preservative and distilled water according to the formula in the embodiment 1, 80% (80 kg) of the salix leaf hydroalcoholic extract and 0.1% (0.2 kg) of carvacrol adsorbed by silicon dioxide aerogel; 0.5 percent (0.5 kg) of ethyl p-hydroxybenzoate (food preservative) and 18.4 percent (18.4 kg) of distilled water, and the Chinese medicinal disinfection composition of the embodiment 1 can be prepared by respectively putting the weighed raw materials into a homogenizer at the rotating speed of 5000/min and homogenizing and stirring for 15 min.
Example 2
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof
The traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight (100 kg grade): 0.5% of hawthorn kernel extract; salix leaf hydroalcoholic extract 90.0%; carvacrol 0.2%; 0.3% of food preservative; 9.0 percent of water.
The preparation method of the traditional Chinese medicine disinfection composition comprises the following specific steps:
step 1: re-refluxing/separating and refining of haw kernel extract
The method is the same as example 1, 1kg of hawthorn kernel essence stock solution is taken and mixed with distilled water according to the proportion of 1.
And 2, step: preparation of antioxidant willow leaf extract
The method is the same as example 1, soaking 4kg of dried willow leaves in 60kg of ethanol-water solution for 24 hours, wherein the ethanol concentration is 8%, refluxing and extracting for 2 hours at 90 ℃ after soaking for 24 hours, squeezing and filtering, and collecting about 52kg of filtrate; and adding 40kg of ethanol-water solution, extracting for 1 hour under reflux, squeezing, filtering, collecting 40kg of filtrate, mixing the two filtrates, adding 0.1% of citric acid and 0.1% of ascorbic acid, stirring to dissolve, cooling in water bath, sealing, storing in dark place, measuring pH to 5.13, and keeping fragrant smell.
And step 3: preparation of silica aerogel substituted solvent/adsorbed carvacrol
The method is the same as example 1, and 0.4kg of silica gel powder with the content of 50% is weighed for standby.
And 4, step 4: preparation of the traditional Chinese medicine disinfection composition
The method is the same as that in example 1, according to the formula in example 1, 0.5% (0.5 kg) of hawthorn seed essence reflux separation liquid, 90% (90 kg) of salix leaf hydroalcoholic extract, 0.2% (0.4 kg) of carvacrol adsorbed by silica aerogel, 0.3% (0.3 kg) of ethyl p-hydroxybenzoate and 9.0% (9.0 kg) of purified water are weighed, and the weighed raw materials are respectively put into a homogenizer at the rotation speed of 4000r/min and are homogenized and stirred for 20min, so that the traditional Chinese medicine disinfection composition in example 2 of the invention can be prepared.
Example 3
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof
The traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight (100 kg grade): 0.8% of hawthorn kernel extract; 10.0 percent of salix leaf hydroalcoholic extract; 1.0% of carvacrol; 0.2% of food preservative; and (5) 88.0 percent of water.
The preparation method of the traditional Chinese medicine disinfection composition comprises the following steps:
step 1: re-refluxing/separating and refining of haw kernel extract
The method is the same as example 1, 2kg of hawthorn seed extract is mixed with distilled water according to the proportion of 1.
Step 2: preparation of willow leaf extract
The method is the same as example 1, 1kg of dried willow leaves are soaked in 15kg of ethanol-water solution for 24 hours, and the ethanol content in the water solution is 8 percent. Soaking willow leaves for 24 hours, performing reflux extraction at 90 ℃ for 3 hours, squeezing and filtering, and collecting 13kg of filtrate; and adding 10kg of ethanol-water solution, extracting for 1 hour under reflux, squeezing, filtering, collecting filtrate, mixing the two filtrates, adding 0.1% citric acid and 0.1% ascorbic acid, stirring to dissolve, cooling in water bath, sealing, storing in dark place, measuring pH to 5.0, and keeping the fragrance for use.
And step 3: preparation of silica aerogel substituted solvent/adsorbed carvacrol
The method is the same as example 1, 2.0kg of silica gel powder with the content of 50% is weighed for standby.
And 4, step 4: preparation of the Chinese medicinal disinfectant composition
The method is the same as the example 1, and 0.8 percent (0.8 kg) of hawthorn seed extract reflux separation liquid, 10 percent (10 kg) of salix leaf hydroalcoholic extract and 1.0 percent (2.0 kg) of carvacrol adsorbed by silica aerogel are weighed according to the formula of the example 2; ethyl p-hydroxybenzoate (food preservative) 0.2% (0.2 kg): 88.0 percent (88.0 kg) of purified water, respectively putting the weighed raw materials into a homogenizer at the rotation speed of 4000r/min, and homogenizing and stirring for 40min to obtain the traditional Chinese medicine disinfection composition disclosed by the embodiment 3 of the invention.
Example 4
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof
The traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight (100 kg grade): 2% of hawthorn seed extract; salix leaf hydroalcoholic extract 90%; carvacrol 0.2%; 0.3% of food preservative; and 7.5% of water.
The preparation method of the traditional Chinese medicine disinfection composition comprises the following specific steps:
step 1: re-refluxing/separating and refining of haw kernel extract
The method is the same as example 1, 2.0kg of hawthorn seed extract is mixed with distilled water according to the proportion of 1.
And 2, step: preparation of willow leaf extract
The method is the same as example 1, 4kg of dried willow leaves are weighed, soaked in 80kg of ethanol-water solution for 24 hours, wherein the ethanol content is 5 percent, and after the willow leaves are soaked for 24 hours, the willow leaves are refluxed and extracted for 2 hours at the temperature of 90 ℃, squeezed and filtered, and about 72kg of filtrate is collected; adding the filter residue into 40kg ethanol-water solution, extracting under reflux at 90 deg.C for 1 hr, squeezing, filtering, collecting about 40kg filtrate, mixing filtrates, adding 0.1% citric acid and 0.1% ascorbic acid, stirring for dissolving, cooling in water bath, sealing, and storing in dark place. The pH was found to be 5.36, with an aromatic odor. Weighing 80kg of salix leaf hydroalcoholic extract for later use.
And step 3: preparation of silica aerogel substituted solvent/adsorbed carvacrol
The method is the same as example 1, and 0.4kg of silica gel powder with the content of 50% is weighed for standby.
And 4, step 4: preparation of the traditional Chinese medicine disinfection composition
The method is the same as example 1, according to the formula of example 3, 2% (2.0 kg) of the hawthorn seed extract reflux separation liquid, 90% (90 kg) of the salix leaf hydroalcoholic antioxidant extract, 0.2% (0.4 kg) of carvacrol adsorbed by silica aerogel, 0.3% (0.3 kg) of ethyl p-hydroxybenzoate and 7.5% (7.5 kg) of purified water are weighed, the weighed raw materials are respectively put into a homogenizer at the rotating speed of 3000r/min, and the traditional Chinese medicine disinfection composition disclosed by the invention in example 4 is prepared by homogenizing and stirring for 30 min.
Example 5
Quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people and preparation method thereof
The traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight (100 kg grade): 3% of hawthorn seed extract; 10% of salix leaf hydroalcoholic extract; 1.0% of carvacrol; 0.2% of food preservative; and 85.8 percent of water.
The preparation method of the traditional Chinese medicine disinfection composition comprises the following specific steps:
step 1: re-refluxing/separating and refining of haw kernel extract
The method is the same as example 1, 3.0kg of hawthorn seed extract is mixed with distilled water according to the proportion of 1.
Step 2: preparation of willow leaf extract
The procedure is as in example 1, and 1kg of dried willow leaves are soaked in 15kg of an ethanol-water solution containing 8% ethanol for 24 hours. Soaking willow leaves for 24 hours, performing reflux extraction for 3 hours at the temperature of 90 ℃, squeezing and filtering, and collecting about 13kg of filtrate; extracting with 10kg ethanol-water solution under reflux for 1 hr, squeezing, filtering, collecting filtrate, mixing the filtrates, adding 0.1% citric acid and 0.1% ascorbic acid, stirring to dissolve, cooling in water bath, sealing, and storing in dark place. The pH was measured to be 5.0 and the fragrance was fragrant for future use.
And 3, step 3: preparation of silica aerogel substituted solvent/adsorbed carvacrol
The method is the same as example 1, 2.0kg of silica gel powder with the content of 50% is weighed for standby.
And 4, step 4: preparation of the Chinese medicinal disinfectant composition
The method is the same as that in example 1, 3 percent (3.0 kg) of hawthorn seed extract reflux separation liquid, 10 percent (10 kg) of saligenin antioxidant extract, 1.0 percent (2.0 kg) of carvacrol adsorbed by silica aerogel, 0.2 percent (0.2 kg) of ethyl p-hydroxybenzoate and 85.8 percent (85.8 kg) of purified water are weighed according to the formula in example 4, the weighed raw materials are respectively put into a homogenizer at the rotating speed of 3000r/min, and the traditional Chinese medicine disinfection composition in the embodiment 5 of the invention is prepared after the homogenization and stirring for 40 min.
Performance test
Experimental example 1
Screening experiment of neutralizer for Chinese medicinal disinfectant composition of the invention:
action of a neutralizing agent: and neutralizing the bactericidal effect of the disinfectant, adding a neutralizing agent to stop the disinfectant from continuing to sterilize when the specified sterilization time is reached so as to evaluate the sterilization effect of the disinfectant within the specified time, and preventing the sterilization time of a disinfectant of an individual experimental group from exceeding the specified time due to the delay of test operation to influence the evaluation of the sterilization effect.
According to the disinfection technical specification (2002 edition) 2.1.1.5.1 neutralizer identification test, the attached neutralizer initial selection test and the 2.1.1.5.5 neutralizer suspension quantitative identification test operation procedure and method, the traditional Chinese medicine disinfection composition neutralizer screening and identification test is carried out, and the method comprises the following steps:
test samples: example 1 provides a sample of a Chinese medicinal disinfecting composition
Test strains: escherichia coli 8099, staphylococcus aureus ATCC6538, purchased from Beijing culture Collection, inc.; candida albicans ATCC10231 was purchased from the Shanghai depositary Biotechnology center.
Neutralizing agent: 0.1mol/L PBS solution; 3.9% D/E neutralized broth in 0.1mol/LPBS solution.
The test method comprises the following steps: quantitative killing test by suspension method according to disinfection technical Specification (2022 edition)
And (3) test results:
TABLE 1 results of 0.1mol/L PBS solution as neutralizer identification test
As shown in table 1, the test results: a neutralizer identification test is carried out according to a method of 5.4.4 in the national standard GB/T38502-2020, a 0.1mol/LPBS solution is used as the neutralizer, the residual effect of the traditional Chinese medicine disinfection composition provided in the example 1 on the test bacteria cannot be effectively neutralized, and the neutralized product has obvious influence on the test bacteria.
TABLE 2.9 The% D/E0.1 mol/LPBS solution of the neutralization broth as the result of the neutralizer identification test
As shown in table 2, the test results: and carrying out a neutralizer identification test according to a method of 5.4.4 in the national standard GB/T38502-2020. The residual effect of the disinfectant composition of example 1 on the test bacteria was effectively neutralized using a 3.9% by volume D/E neutralization broth solution of 0.1mol/LPBS as a neutralizing agent, and the neutralizing agent, the neutralized product had no significant effect on the test bacteria.
TABLE 3.9% D/E neutralization Broth Medium 0.1mol/LPBS solution as neutralizer identification test results
As shown in table 3, the test results: and carrying out a neutralizer identification test according to a method of 5.4.4 in the national standard GB/T38502-2020. The residual effect of example 1 on the test bacteria was effectively neutralized using a 3.9% by weight D/E neutralization broth solution of 0.1mol/LPBS as a neutralizing agent, and the neutralizing agent, the neutralized product had no significant effect on the test bacteria.
In conclusion, it was confirmed from the results of the neutralizer suspension quantitative determination test that the 0.1mol/LPBS solution could not be used as the neutralizer for the disinfection effect experiment of example 1, while the 0.1mol/LPBS solution of 3.9% D/E neutralization broth medium could effectively neutralize the disinfection composition of example 1. Accordingly, the applicant of the present invention used a 0.1mol/LPBS solution of 3.9% by weight of D/E neutralized broth as a neutralizer in the disinfection effect test of the disinfectant according to the present invention.
Experimental example 2
Test of the sterilizing rate and effect of the Chinese medicinal sterilizing composition prepared by the invention
(1) The traditional Chinese medicine disinfection composition prepared by the invention has the effect of killing escherichia coli and staphylococcus aureus
Test samples: the traditional Chinese medicine disinfection composition sample provided in example 1.
Test strains: escherichia coli 8099, staphylococcus aureus ATCC6538, purchased from Beijing culture Collection, inc.; candida albicans ATCC10231 was purchased from the Shanghai depositary Biotechnology center.
Neutralizing agent: 0.1mol/L PBS solution; 3.9% of a 0.1mol/LPBS solution of the D/E neutralization broth.
The test method comprises the following steps: refer to the sterilization technical Specification (2022 edition) suspension method quantitative kill test.
TABLE 4 Disinfection Effect test of the Chinese medicinal Disinfection composition prepared in example 1 at various concentrations on Escherichia coli
It is necessary to say that: the applicant of the present invention considers the problem of practicality, namely: the transmission speed of the new corona pneumovirus in the air and between people is very high, and if the new corona pneumovirus is close to or faces to each other, the transmission speed of the new corona pneumovirus can be counted in seconds, so that a non-toxic disinfection product matched with the transmission speed of the virus is urgently needed in public places, and therefore, when the disinfection action time and standard of the disinfection product are designed, the shortest action time of the air disinfectant is set to be 30 S (ii) a The time limit of the disinfection effect of the object surface is set to be 5min, and the applicant of the invention believes that if the air disinfection effect is slowed for 30s, the practical value of air disinfection and prevention and treatment of respiratory infectious diseases such as new coronary pneumonia, pulmonary tuberculosis, legionella pneumophila and the like is lost.
The disinfection rate test of the traditional Chinese medicine disinfection composition is shown in tables 1 and 2, and the test result is as follows: the 100% anhydrous diluted disinfectant in the embodiment 1 and the 80% anhydrous diluted disinfectant in the embodiment 1 are subjected to a disinfection effect test, the effect is 30s, and the killing logarithm value of escherichia coli is not less than 5; the 50 percent of the water diluent of the embodiment 1 acts for 1min, and the killing logarithm value of the escherichia coli can be more than or equal to 5; the 30 percent of the disinfectant water diluent of the embodiment 1 acts for 2min, and the killing log value of the disinfectant water diluent to escherichia coli can be more than or equal to 5;20 percent of the disinfectant diluent of the embodiment 1 acts for 10min, and the killing logarithm value of the disinfectant on escherichia coli is more than or equal to 5;10% of the disinfectant diluent of the embodiment 1 and 5% of the disinfectant diluent of the embodiment 1, the action is 10min, and the killing logarithm value of the Escherichia coli is still less than 5 (not meeting the requirement of a disinfection standard).
TABLE 5 test of sterilizing effect of the Chinese medicinal sterilizing composition prepared in example 1 on Staphylococcus aureus at various concentrations
And (3) test results: as shown in Table 5, the disinfection effect test is carried out on 100% of the anhydrous disinfectant of example 1, 80% of example 1+20%, 50% of example 1+50% of water and 30% of example 1+70% of water, and the killing logarithm value of the staphylococcus aureus can be more than or equal to 5 after the action of 30 s; 20% of the disinfectant of example 1+ 80% of purified water, and acting for 1min;10 percent of the embodiment 1+90 percent of water acts for 10min, and the killing log value of the staphylococcus aureus is more than 5 (the killing log value is of no practical significance for preventing and controlling the new corolla pneumovirus); 5% of example 1+95% of water, acting for 10min, the killing logarithm value to staphylococcus aureus is less than 5 (no practical significance).
(2) The traditional Chinese medicine disinfection composition prepared by the invention can be used for testing the activity of resisting influenza A virus
The test method comprises the following steps: adding MDCK cell culture fluid into 96-well cell culture plate, culturing to obtain single layer, discarding supernatant, washing with PBS buffer twice, and adding 100 μ L each of the Chinese medicinal composition prepared in examples 1-4 and H1N1 virus solution into each well; additionally, adding blank control group and virus control group at 37 deg.C and saturation humidity of 70% in 5% CO 2 After incubation in an incubator for 72 hours, WST-1 solution with a concentration of 0.5mM was added to each well, and after 4 hours, absorbance was measured at 450nm with a microplate reader to calculate IC 50 The value is obtained.
IC 50 The calculation method is as follows: cell survival rate = (cell control well OD value-dosing well OD value) × 100%;
the concentration corresponding to 50% cell viability was determined as IC50 value by plotting the drug concentration on the X-axis and the cell viability on the Y-axis.
TABLE 6 IC50 values after treatment of the Chinese medicinal disinfecting compositions prepared in accordance with the present invention
As shown in table 6, the Chinese medicinal sterilizing compositions prepared in examples 1 to 5 of the present invention had sterilizing activity against influenza a virus.
Experimental example 3 verification experiment of synergistic effects of components of Chinese medicinal disinfectant composition
(1) The carvacrol has synergistic effect with other components
In order to prove that carvacrol and a food preservative used in the traditional Chinese medicine disinfection composition have synergistic effects with other components, the applicant of the patent compares the disinfection effects of the traditional Chinese medicine disinfection compositions prepared in the example 1 and the control group 2 on escherichia coli and staphylococcus aureus, the control group 1 and the example 1 are only different in that all components do not contain carvacrol, and the rest components, the component content and the preparation method are the same as those in the example 1, and the specific experimental method refers to the experimental example 1; the control group 2 is different from the example 1 only in that all the components do not contain crataegus pinnatifida essence and saligenin extract (only containing carvacrol), and the rest components, the content of the components and the preparation method are the same as those of the example 1, and the specific experimental method refers to the experimental example 1.
As shown in table 7, the test results: the traditional Chinese medicine disinfection composition prepared in the embodiment 1 (100%) has the effect of 30s, and the killing logarithm value of escherichia coli can reach the disinfection standard of more than or equal to 5; however, the control group 1 and the control group 2 both acted for 5min, and thus it was judged that: 1) The effect of using carvacrol only or using carvacrol is limited and retarded, and the sterilization rate is obviously inferior to that of the important mixed preparation of the disinfection composition prepared by the invention; 2) The hawthorn seed essence and the carvacrol have obvious synergistic relationship.
TABLE 7 test of the synergistic effect of carvacrol and the disinfecting effect on E.coli
TABLE 8 synergistic effect of carvacrol and Sterilization Effect test on Staphylococcus aureus
As shown in table 8, the test results: the traditional Chinese medicine disinfection composition prepared in the embodiment 1 has (100%) action for 30s, and the killing logarithm value of staphylococcus aureus can reach the disinfection standard of more than or equal to 5; however, the control group without carvacrol and food preservative can make the killing logarithm value of staphylococcus aureus be more than 5 after 5min of action, and the difference is great compared with the test group, so the carvacrol and the food preservative can improve the sterilizing effect of the hawthorn seed extract and the saligenin extract on the staphylococcus aureus.
(2) The hawthorn seed extract and other components have synergistic effect
In order to prove that the hawthorn seed extract used in the traditional Chinese medicine disinfection composition has synergistic effect with other components, the applicant of the patent carries out disinfection and killing on escherichia coli, staphylococcus aureus and candida albicans by comparing the traditional Chinese medicine disinfection composition prepared in the embodiment 1 with the control group 1, wherein the control group 1 only differs from the embodiment 1 in that all the components do not contain the hawthorn seed extract, and the control group 2 only differs from the embodiment 1 in that the salix leaf extract and carvacrol (only containing the hawthorn seed extract) do not contain the traditional Chinese medicine disinfection composition, and the rest components, the component contents and the preparation method are the same as those of the embodiment 1.
The inhibition effect of the experiment on escherichia coli, staphylococcus aureus and candida albicans is achieved by adopting a method of inhibiting bacteria ring (qualitative) in 2002 edition 'disinfection technical Specification', a preparation with the hawthorn seed essence removed is adsorbed by a filter paper sheet with the diameter of 5mm, distilled water is adsorbed by a blank control filter paper, and the experimental result is shown in the following table:
TABLE 9 analysis of the bacteriostatic and synergistic effects of Hawthorn kernel extract
The diameter of the bacteriostatic ring is in direct proportion to the sterilization rate or the disinfection effect, the larger the diameter of the bacteriostatic ring is, the higher the sterilization rate is, the result analysis shows that the specific bacteriostatic ring with the diameter of 7mm has the bacteriostatic action, the bacteriostatic ring with the diameter of 10mm has the stronger effect, the bacteriostatic ring with the diameter of 20mm has the stronger effect, and the control group 1 has the bacteriostatic action on escherichia coli and staphylococcus aureus; has no bacteriostatic ring and bacteriostatic action on candida albicans and has a weakened effect, and the control group 2 has no bacteriostatic action on escherichia coli, staphylococcus aureus and candida albicans, which shows that the combination of the hawthorn seed extract, the willow leaf extract and carvacrol obviously improves the killing action on pathogenic bacteria and has a synergistic effect.
(3) Experiment of bacteriostatic effect of salix leaf hydroalcoholic extract monomer
In this experimental example, all other ingredients in the formulation of example 1 of the present invention were removed, and only the salix leaf hydroalcoholic extract component was retained, and the experimental method was performed with reference to experimental example 3 (2).
TABLE 10 experiment of bacteriostatic effect of the willow leaf extract used alone in example 1
The results show that the diameter of the saligenin extract monomer on the bacteriostatic ring of escherichia coli is 6.0mm, the diameter of the bacteriostatic ring of staphylococcus aureus is 7.5mm, the bacteriostatic ring of candida albicans is not generated, 5mm filter paper sheets have no bacteriostatic ring in contrast, and the number of colonies growing on the surface cannot be counted, so that the situation that only the saligenin extract in example 1 is used, the saligenin extract has no bacteriostatic action on escherichia coli, the bacteriostatic action on staphylococcus aureus is weak, and the bacteriostatic action on candida albicans is avoided can be judged, and the number of blank control colonies in the experiment cannot be counted, so that the situation that the bactericidal effect is poor when the saligenin extract is used alone can be inferred.
(4) Synergistic preservative effect experiment of carvacrol and preservative
The traditional Chinese medicine disinfection composition is a water-soluble plant formula and a preparation, if no preservative is added, the preparation starts to go moldy and grow mildews after being stored for 20 days in hot summer (30-40 ℃), the color of the preparation turns green, and peculiar smell is generated. Therefore, the invention selects ethyl hydroxybenzoate (alias: ethylparaben) as a preservative, the preservative is stored at 35 ℃ within the application range of 0.2-1.0%, and the shelf life of the product is 3 months. If the ethylparaben and the carvacrol are used in a compound way, the preservative shelf life can be prolonged to 18 months.
The experiment example of the synergistic antiseptic effect of carvacrol and ethylparaben is to remove all other ingredients in the formula of the invention in the embodiment 1, only carvacrol and ethylparaben are reserved, the group containing carvacrol and ethylparaben in the embodiment 1 (the control group 1), the group containing carvacrol and ethylparaben in the embodiment 1 (the control group 2) and the group containing carvacrol and ethylparaben in the embodiment 1 (the embodiment 1) are respectively arranged, the natural sample reservation is adopted to carry out the mildew-proof and quality guarantee period tests, and the product is observed to have mold breeding, discoloration and odor in four seasons (18 months) of spring, summer, autumn and winter,
TABLE 11 synergistic preservative efficacy analysis results for carvacrol plus preservative
As shown in table 11, the effect of the traditional Chinese medicine disinfection composition prepared in example 1 is significantly better than that of the control group 1 and the control group 2, which indicates that carvacrol and ethylparaben have excellent combination effect.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The present invention and its embodiments have been described above, and the description is not intended to be limiting, and what is shown in the drawings is only one embodiment of the present invention, and the practical application is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A quick-acting traditional Chinese medicine disinfection composition capable of being used in space with people is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
0.5 to 3.0 percent of hawthorn kernel essence;
10 to 90 percent of salix leaf hydroalcoholic extract;
carvacrol 0.1-1.0%;
0.2 to 1.0 percent of food preservative;
the balance of water.
2. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the space with people according to claim 1, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
0.5% of hawthorn kernel extract;
salix leaf hydroalcoholic extract 90.0%;
carvacrol 0.2%;
0.3% of food preservative;
9.0 percent of water.
3. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the space with people according to claim 2, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
0.8% of hawthorn kernel extract;
10.0 percent of salix leaf hydroalcoholic extract;
1.0% of carvacrol;
0.2% of food preservative;
and (5) 88.0 percent of water.
4. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the human space according to claim 3, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
1.0% of hawthorn seed extract;
salix leaf hydroalcoholic extract 80%;
carvacrol 0.1%;
0.5% of food preservative;
and (4) 18.4% of water.
5. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the human space according to claim 4, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
2.0% of hawthorn seed extract;
salix leaf hydroalcoholic extract 90%;
carvacrol 0.2%;
0.3% of food preservative;
and 7.5% of water.
6. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the human space according to claim 5, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following raw materials in percentage by weight:
3.0% of hawthorn seed extract;
10.0% of salix leaf hydroalcoholic extract;
1.0% of carvacrol;
0.2% of food preservative;
and 85.8 percent of water.
7. The quick-acting traditional Chinese medicine disinfection composition capable of being used in the human space according to claim 6, which is characterized in that: the traditional Chinese medicine disinfection composition comprises the following applications:
(1) The traditional Chinese medicine disinfection composition can be used in human space;
(2) Applied to surface disinfection treatment;
(3) The composition is applied to killing one or more of escherichia coli, staphylococcus aureus, staphylococcus albus, influenza A virus and coronavirus;
(4) The preparation is used for preventing respiratory infectious diseases, wherein the pathogenic bacteria of the respiratory infectious diseases comprise one or more of neocoronarism, influenza, tuberculosis and legionella pneumophila.
8. The quick acting traditional Chinese medicine disinfecting composition for use in occupied space of claim 7, characterized in that: the neutralizer used in the disinfection effect test of the traditional Chinese medicine disinfection composition is a 0.1mol/L PBS solution of 3.9% D/E neutralization broth culture medium.
9. A method for preparing the quick-acting traditional Chinese medicine disinfecting composition used in the space where people are present according to claim 8, which is characterized in that: the preparation method of the traditional Chinese medicine disinfection composition comprises the following steps:
(1) Preparation of the hawthorn seed extract
Mixing the raw material of the hawthorn seed powder with distilled water according to the proportion of 1-4, and distilling for 1-3 times under reduced pressure at 40-65 ℃ to obtain colorless and transparent distillate or dark brown concentrate;
(2) Preparation and antioxidation of the salix leaf hydroalcoholic extract
Picking leaves of Salix babylonica, naturally airing or sun drying, soaking in 15-20 times volume of ethanol-water solution containing 5-10% (v/v) ethanol for 24 hr, reflux extracting at 90 deg.C for 2 hr, squeezing, filtering, and collecting filtrate; adding 10 times of water into the filter residue, continuously extracting under reflux at 90 deg.C for 1 hr, squeezing and filtering, collecting filtrate, mixing the filtrates, and making saligenin crude extract; immediately adding 0.1% (v/v) citric acid and 0.1% (v/v) ascorbic acid, stirring until the citric acid and the ascorbic acid are completely dissolved, cooling in water bath, sealing, and storing in dark place;
(3) Preparation of the carvacrol
Mixing and dissolving a carvacrol raw material and absolute ethyl alcohol according to a volume ratio of 1;
(4) Preparation of the Chinese medicinal disinfectant composition
Mixing the hawthorn kernel essence prepared in the step (1), the salix leaf hydroalcoholic extract prepared in the step (2), the carvacrol prepared in the step (3), a food preservative and water according to a ratio, and homogenizing and stirring to obtain the traditional Chinese medicine disinfection composition, wherein the traditional Chinese medicine disinfection composition can be prepared into a disinfectant using dosage form according to a conventional process, and the dosage form comprises one of aqua, gel, solid and powder.
10. The method for preparing a quick-acting Chinese medicinal disinfectant composition for use in occupied spaces according to claim 9, wherein: the pH of the distillate in step (1) of claim 9 is 2.0-3.0, the organic acid content of the distillate is 4-9%, and the pH of the salix leaf hydroalcoholic extract in step (2) of claim 9 is 4.5-5.5.
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| CN1259990A (en) * | 1997-05-30 | 2000-07-12 | 普罗格特-甘布尔公司 | Disinfecting compositions and processes for disinfecting surface |
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| DE102020125669A1 (en) * | 2020-10-01 | 2022-04-07 | Hans Karrer Gmbh | Antiviral disinfectant hand gel |
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2022
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1259990A (en) * | 1997-05-30 | 2000-07-12 | 普罗格特-甘布尔公司 | Disinfecting compositions and processes for disinfecting surface |
| CN104082353A (en) * | 2014-07-04 | 2014-10-08 | 李钟� | Pure traditional Chinese medicine disinfectant and preparation method thereof |
| CN104094979A (en) * | 2014-07-04 | 2014-10-15 | 李钟� | Willow leaf for preparing sanitizer |
| DE102020125669A1 (en) * | 2020-10-01 | 2022-04-07 | Hans Karrer Gmbh | Antiviral disinfectant hand gel |
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