CN115634924B - Tea garden polluted soil restoration and treatment method - Google Patents
Tea garden polluted soil restoration and treatment method Download PDFInfo
- Publication number
- CN115634924B CN115634924B CN202211210104.6A CN202211210104A CN115634924B CN 115634924 B CN115634924 B CN 115634924B CN 202211210104 A CN202211210104 A CN 202211210104A CN 115634924 B CN115634924 B CN 115634924B
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- soil
- actinomycetes
- microbial
- tea garden
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002689 soil Substances 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims abstract description 51
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 123
- 230000000813 microbial effect Effects 0.000 claims abstract description 67
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 62
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 50
- 241001122767 Theaceae Species 0.000 claims abstract description 48
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000004321 preservation Methods 0.000 claims abstract description 7
- 238000005067 remediation Methods 0.000 claims description 36
- 230000001580 bacterial effect Effects 0.000 claims description 19
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 35
- 239000002131 composite material Substances 0.000 description 27
- 239000010949 copper Substances 0.000 description 21
- 239000003344 environmental pollutant Substances 0.000 description 13
- 231100000719 pollutant Toxicity 0.000 description 13
- 244000005700 microbiome Species 0.000 description 11
- 150000002500 ions Chemical class 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 8
- 239000011651 chromium Substances 0.000 description 8
- 239000011133 lead Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 230000008439 repair process Effects 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 229910052793 cadmium Inorganic materials 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 229910052759 nickel Inorganic materials 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241001446247 uncultured actinomycete Species 0.000 description 4
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002420 orchard Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- ODLMAHJVESYWTB-UHFFFAOYSA-N propylbenzene Chemical compound CCCC1=CC=CC=C1 ODLMAHJVESYWTB-UHFFFAOYSA-N 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 241001467490 Agromyces Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- -1 Cu (II) Chemical class 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000396668 Lechevalieria Species 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 240000002044 Rhizophora apiculata Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000007633 bacillus mucilaginosus Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 210000004709 eyebrow Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000005468 ion implantation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- NBTOZLQBSIZIKS-UHFFFAOYSA-N methoxide Chemical compound [O-]C NBTOZLQBSIZIKS-UHFFFAOYSA-N 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a method for restoring and treating contaminated soil in a tea garden, which adopts a microbial restoration agent to be applied into the contaminated soil in the tea garden for treating the heavy metal contaminated soil, wherein the microbial restoration agent consists of heavy metal-resistant actinomycetes NJ07-19, heavy metal-enriched actinomycetes CS-16 and nano ferroferric oxide according to the weight ratio of 3:1:2; wherein, the preservation number of the heavy metal-resistant actinomycetes NJ07-19 is CCTCC No: m2015737; the collection number of the heavy metal enrichment actinomycetes CS-16 is CGMCC No.21594. The invention fully plays the microbial restoration effect of two actinomycetes, the actinomycetes can play roles of mutual promotion and synergy, meanwhile, the nano ferroferric oxide can play a good carrier role, the two microbial agents are efficiently loaded and fully keep the activity, the microbial restoration effect is played for a long time, and the invention plays a good restoration effect on complex soil polluted by various heavy metals in tea gardens.
Description
Technical Field
The invention belongs to the technical field of soil heavy metal pollution treatment, and particularly relates to a tea garden polluted soil restoration treatment method.
Background
With the rapid development of modern industry and the influence of human production activities, the phenomenon that the soil in nature is damaged is continuously aggravated, and the heavy metal pollution in the soil is more serious. The heavy metal pollution of the soil not only seriously affects the soil quality and the soil fertility improvement, but also endangers the ecology, the food safety and the human health.
Heavy metal pollution refers to pollution of metals with specific gravity greater than 5 or other types of compounds to the environment, and the heavy metal pollutants in soil resources mainly comprise lead, zinc, copper, cadmium, arsenic, mercury, chromium, nickel, manganese and the like.
The main cause of heavy metal pollution in soil is human factor. The heavy metal content in the soil is increased to a certain extent in industrial and mining enterprises, agricultural production activities, transportation and the like, and in addition, a large amount of heavy metals such as lead are carried in electronic products, and the heavy metals are not recycled after being abandoned, so that the heavy metals are easy to penetrate into the soil to cause pollution.
Heavy metal pollutants are easy to accumulate due to high toxicity and difficult degradation, can be enriched in soil and organisms, cause pollution to the soil and crops, have great harm to the growth and quality of the crops, and have potential danger of damaging human and animal health after being absorbed and enriched into a food chain by the crops. The heavy metal contaminated soil has concealment, long-term property and irreversibility, and is an important pollutant for affecting the safety of an ecological system. Therefore, the treatment of heavy metal pollution becomes a matter that people protect natural resources, improve living environment and ensure human health and are forced to be in eyebrows.
At present, methods for treating heavy metal contaminated soil mainly comprise a physical repair method, a chemical repair method and a biological repair method. The physical repair method has the problems of narrow application range and high input energy components in the treatment process. The chemical remediation method mainly changes the form of heavy metal pollutants in soil, and does not change the total amount and total concentration of heavy metals in the soil greatly, and the heavy metals subjected to improvement treatment still have the possibility of reactivation, so that secondary pollution is easily caused to the soil.
While bioremediation is a method that is completely different from the above-described physical and chemical remediation methods. Compared with the traditional repairing method, the biological repairing method is widely paid attention to due to the advantages of environment friendliness, high efficiency, low cost and the like. The widely studied microbial remediation method is to add a microbial agent into contaminated soil, and reduce the effective concentration of heavy metals in the soil by utilizing the affinity, adsorption and conversion effects of the microbial agent on the heavy metals. The microbial remediation method for treating the heavy metal pollution of the soil has the advantages of low cost, high efficiency, no need of stopping tillage, in-situ remediation, small influence on the soil environment, improvement of the soil fertility and the like, and becomes a hotspot in the research field of restoring the heavy metal pollution of the soil.
Although a great deal of research is carried out on a microbial remediation method, the existing research is mainly focused on the effect between a certain microbial agent and a plurality of different microbial agents to realize the remediation of heavy metal pollution, and the microbial remediation method can only treat a certain pollutant or a few pollutants and has limited exerted remediation effect. However, for heavy metal pollution of soil, the heavy metal pollution is generally polluted by multiple heavy metals at the same time, the heavy metal pollutant components in the soil are complex, the single restoration means often has poor restoration effect on complex pollutant components, and the high-efficiency restoration treatment can be realized only for a certain pollutant, but the restoration effect on other pollutants is poor.
For example, patent document CN 105036352B provides a microorganism method for repairing aromatic hydrocarbon-heavy metal ion composite pollution, which is to uniformly mix pseudomonas putida concentrated bacterial liquid, saccharomycete concentrated bacterial liquid and bacillus mucilaginosus concentrated bacterial liquid according to a proportion to prepare composite concentrated bacterial liquid, and then add the composite concentrated bacterial liquid into a solution containing aromatic hydrocarbon and heavy metal ions to effectively degrade aromatic hydrocarbon and mineralize heavy metal ions to form stable mineral carbonate. However, the method is mainly aimed at removing and repairing propylbenzene and zinc ions, the repairing effect of other heavy metal ions is not researched, and the removing effect of pollutants with complex compositions is not researched and reported.
For example, patent document CN 107129942A discloses a method for culturing composite flora and producing acid rapidly and the obtained composite flora, and the method uses the composite flora, but the composite flora only has better removal efficiency for removing heavy metal cadmium in soil, and the application range is still limited.
The microbial remediation method mainly depends on obtaining microorganisms with high activity and high metabolic capacity, but due to the complexity of the microorganisms, the specific application of the microbial remediation method to soil heavy metal pollution by domestic and foreign scholars is still in deep, and the influence of the interaction between plants and microorganisms or between biological adsorbents on plant growth, heavy metal absorption and remediation efficiency is still to be further studied. Therefore, the existing restoration means still need to be further researched so as to provide a heavy metal restoration agent with better restoration effect and a restoration treatment method for soil.
Because the demand of people for tea leaves is large, especially in places such as Sichuan and Fujian, the tea planting is popular, and the tea garden has the characteristics of wide planting area and high soil quality requirement. However, heavy metals accumulate in tea garden soil and easily enter human bodies through tea food chains, so that a series of tea quality and safety problems are caused. Therefore, whether a repair treatment method aiming at the heavy metal pollution of the tea garden soil can be found becomes a technical problem to be solved urgently.
Disclosure of Invention
The invention aims to solve the technical problems, and provides a method for restoring and treating contaminated soil in tea gardens. The invention aims to solve the problems that the existing microorganism repairing method cannot fully exert the synergistic repairing effect among different microorganism bactericides, so that the existing microorganism repairing method has lower removing efficiency on complex heavy metal pollution components and can only treat one or a few heavy metal pollutants.
The invention provides a method for restoring and treating tea garden polluted soil, which comprises the steps of applying a microbial restoration agent into tea garden soil to treat heavy metal polluted soil, wherein the microbial restoration agent consists of heavy metal-resistant actinomycetes NJ07-19, heavy metal-enriched actinomycetes CS-16 and nano ferroferric oxide according to a weight ratio of 3:1:2; wherein, the preservation number of the heavy metal-resistant actinomycetes NJ07-19 is CCTCC No: m2015737; the collection number of the heavy metal enrichment actinomycetes CS-16 is CGMCC No.21594.
Further, the concentration of the bacterial liquid of the heavy metal resistant actinomycetes NJ07-19 in the microbial remediation agent is 6.0x10 7 The concentration of bacterial liquid of heavy metal enriched actinomycetes CS-16 is 3.5X10 per mL 7 And each mL.
Further, the purity of the nano ferroferric oxide is more than 99.5%, and the average grain diameter is less than or equal to 20nm.
Further, the two actinomycetes are respectively cultured, the two bacterial solutions are mixed after the required bacterial solution concentration is achieved, then nano ferroferric oxide powder is added into the bacterial solutions, and the bacterial solutions are uniformly mixed and then are applied into tea garden soil as a microbial restoration agent.
Furthermore, the microbial remediation agent applied to the tea garden soil accounts for 2-3% of the total weight of the soil.
Further, the heavy metals in the tea garden polluted soil comprise at least one of Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI).
The restoration and treatment method for the contaminated soil in the tea garden provided by the invention fully plays the synergistic effect of various microbial agents in the microbial restoration method, and realizes the treatment of the heavy metal contaminated soil in the tea garden to the greatest extent. According to the invention, a large number of microbial strains are screened, and the method finally obtains the composite microbial agent which is selected from the heavy metal-resistant actinomycetes and the heavy metal-enriched actinomycetes, and the combination of the two actinomycetes can fully play the high-efficiency repairing and synergistic repairing roles of the microbial agent.
On the other hand, as the mechanism of the microbial agent for playing the repairing role in the soil is to keep higher microbial activity, the invention further searches, and finally, the nano ferroferric oxide is selected to be compounded with the two actinomycetes, so that the microbial agent can play a role of a carrier, efficiently fix and keep the activity of the microbial agent, is more beneficial to playing the repairing role of the microbial agent, and has a good synergistic effect. When the nano ferroferric methoxide is not used as a carrier or the dosage proportion is changed, the capability of playing the effect of repairing microorganisms is reduced, and the effect of removing heavy metals is weakened. The repairing method provided by the invention can realize the efficient absorption and removal of various heavy metal ions including Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI) in tea garden soil, plays a good interaction promoting role between two microbial agents and between the microbial agents and a carrier, and greatly improves the repairing effect of the invention.
A heavy metal resistant actinomycete strain Lechevalieria nanjingensis NJ-19 (preservation number: CGMCC No: M2015737) is reported in patent document CN 105861362B, and the original strain is obtained by separating and screening from heavy metal contaminated soil and then by ion implantation physical mutagenesis. The strain can tolerate heavy metals of lead, copper, zinc and nickel, wherein the highest tolerance concentration of lead and copper is 1500mg/L and 600mg/L respectively, and the adsorption rate of heavy metal lead and copper can reach 83% and 56% respectively, so that the strain can be applied to the treatment of heavy metal pollution of water and soil.
The above patent document reports the effect of heavy metal-resistant actinomycete strain NJ07-19 for treating and adsorbing heavy metal ions, however, the patent document has limited studies on the adsorption of heavy metal species, and the adsorption performance thereof has yet to be further improved.
Patent document CN 113201475B discloses an actinomycetes sp.CS-16 (preservation number: CGMCC No. 21594) capable of enriching heavy metals, which is an actinomycetes capable of enriching heavy metals and is screened from Shenzhen Futian mangrove forest natural protection zone and belongs to the genus Agromyces. The actinomycete has good tolerance to nickel and cadmium, and can be used for preparing Ni with concentration less than 160mg/L 2+ Can survive in a concentration environment and can also survive in Cd of less than 15mg/L 2+ The water body and soil heavy metal pollution restoration agent can survive in a concentration environment, and the purpose of restoring the water body and soil heavy metal pollution can be achieved. However, the patent document only discloses that the actinomycetes have good removal rates for Ni and Cd, but cannot be used for efficiently removing other kinds of heavy metal ions.
The inventors of the present invention have unexpectedly found that by compounding the heavy metal-resistant actinomycetes NJ07-19 with the heavy metal-enriched actinomycetes CS-16, the ratio thereof is adjusted, which shows an excellent adsorption-removal ability for original heavy metals, and at the same time, shows a high-efficiency adsorption-removal ability for other various heavy metals. The possible reasons are that the combination of the two actinomycetes plays a role in mutual synergy, fully plays the respective heavy metal adsorption performance, and realizes the effect of efficiently removing the heavy metals.
The invention creatively combines the two actinomycetes, and surprisingly discovers that the actinomycetes play a remarkable role in repairing various heavy metal ions in soil, and obviously reduces the content of various heavy metals in the soil of an orchard.
On the basis of the determination of the method of microbial remediation, the inventors tried to combine it with a microbial carrier in order to more fully remediate heavy metal contamination. After a great deal of experimental research, the inventor combines the microbial restoration agent with nano ferroferric oxide, so that the absorption and removal effects of heavy metals can be further improved, the removal effects are comprehensive, the removal effect of the microbial restoration agent on one heavy metal is not good, and the removal effect of the microbial restoration agent on other heavy metals is poor. Finally, by adopting the microbial remediation method, when various heavy metal ions exist in the tea garden soil at the same time, such as Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI) exist at the same time, the efficient absorption and removal effects are still achieved, the combination of the microbial remediation agent and the carrier plays a good role in interaction, and the nano ferroferric oxide is used as the carrier of the microbial remediation agent, so that the activity and the long-acting performance of the restoration capability of the microbial remediation agent can be better ensured. The original microbial repairing agent cannot realize efficient repairing effect on up to the plurality of metal ions when being singly used.
The beneficial effects of the invention are as follows:
(1) The invention provides a method for repairing and treating heavy metal contaminated soil in a tea garden, which adopts a microorganism composite repairing method, and two actinomycetes are selected for compositing, so that a good synergistic effect is achieved, and the respective microorganism microbial agents can be promoted to better exert repairing effects;
(2) Under the composite synergy of the two actinomycetes, the invention further selects a carrier (nano ferroferric oxide) which has good loading function on the two actinomycetes and can efficiently keep the microbial activity of the actinomycetes, and by the combination, the restoration effect of the actinomycetes on heavy metal pollution is further improved;
(3) The method for restoring and treating the contaminated soil in the tea garden can effectively remove complex pollutants existing in the soil in the tea garden when various heavy metal ions exist simultaneously, such as Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III), cr (VI) and the like.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be specifically described with reference to the following examples, which are provided for explaining and illustrating the present invention only and are not intended to limit the present invention. Some non-essential modifications and adaptations of the invention according to the foregoing summary will still fall within the scope of the invention.
Example 1
1. Preparation of composite microbial repairing agent
(1) Purchasing heavy metal-resistant actinomycetes NJ07-19 (with a preservation number of CGMCC No. M2015737) and heavy metal-enriched actinomycetes CS-16 (with a preservation number of CGMCC No. 21594) from China center for type culture collection and China Committee for culture Collection of microorganisms, respectively, and culturing the heavy metal-resistant actinomycetes NJ07-19 and the heavy metal-enriched actinomycetes CS-16 in a culture medium under culture conditions described in CN 105861362B and CN 113201475B, respectivelyAfter culturing to the logarithmic phase, the bacterial count is measured by adopting a dilution plate count to obtain the bacterial liquid with the concentration of 6.0x10 7 Heavy metal-resistant actinomycetes NJ07-19 suspension with a concentration of 3.5X10 7 individual/mL of heavy metal enriched actinomycete CS-16 suspension;
(2) Taking the suspension of the two actinomycetes, and forming a microbial agent by the heavy metal-resistant actinomycetes NJ07-19 and the heavy metal-enriched actinomycetes CS-16 according to the weight ratio of 3:1 for later use.
(3) Adding nano ferroferric oxide (CAS number: 1317-61-9, molecular weight: 231.53, purity: 99.5%, average particle size less than or equal to 20 nm) into the composite microbial agent, and maintaining the respective weight ratio of 3:1:2 to obtain the composite microbial restoration agent.
And thirdly, mixing the microbial restoration agent and the chemical restoration agent according to the weight ratio of 2:3, and using the mixture as a composite restoration agent for restoration treatment of heavy metal contaminated soil in an orchard.
2. Preparation of test soil
The test soil was collected from standard test fields of Sichuan university of agriculture. The basic physical and chemical properties of the soil are as follows: pH 7.0, organic matter 35.13%, total nitrogen 1.98g.kg -1 0.81 g.kg of total phosphorus -1 25.32 g.kg of available potassium -1 . The soil was not contaminated with heavy metals, and the soil was sieved (2 mm) and sterilized at 120℃for 30min for three consecutive days for further use.
Example 2
The composite microbial remediation agent is used for remediation and treatment of the tea garden heavy metal contaminated soil, and the heavy metal contaminated condition of the tea garden soil to be remediated is simulated as follows:
adding CuSO into the tested soil 4 ·5H 2 O、ZnCl 2 And 3CdSO 2 ·8H 2 O solution so that the initial concentration of Cu (II) in the soil is 500 mg.kg -1 The initial concentration of Zn (II) is 500 mg.kg -1 The initial concentration of Cd (II) is 500 mg.kg -1 . The composite microbial remediation agent is added into the soil sample, the addition amount of the composite microbial remediation agent is 2% of the total weight of the soil sample, and the composite microbial remediation agent and the soil sample are uniformly mixed. Adding water into soil, keeping the water content of the soil at 50%, and oscillating at 110rpm at 25deg.CAnd (5) treating the soil. After 3 days, the soil sample is centrifuged, and the final concentrations of Cu (II), zn (II) and Cd (II) in the soil sample are measured, wherein the test results are 0.88 mg.kg respectively -1 、0.54mg·kg -1 、0.69mg·kg -1 The composite microbial remediation agent provided by the invention has the removal rates of Cu (II), zn (II) and Cd (II) in tea garden soil of 99.83%, 99.9% and 99.87%, respectively, and the removal rate of the remediation method provided by the invention on tea garden soil heavy metal is kept at a higher level under the condition of pollution of 3 mixed heavy metals.
Example 3
The composite microbial remediation agent is used for remediation and treatment of the tea garden heavy metal contaminated soil, and the heavy metal contaminated condition of the tea garden soil to be remediated is simulated as follows:
adding CuSO into the tested soil 4 ·5H 2 O、FeCl 3 ·6H 2 O、3CdSO 2 ·8H 2 O、Pb(NO 3 ) 2 And ZnSO 4 The solution is such that the initial concentration of Cu (II) in the soil is 300 mg.kg -1 The initial concentration of Fe (III) is 300 mg.kg -1 The initial concentration of Cd (II) is 400 mg.kg -1 The initial concentration of Pb (II) is 350 mg.kg -1 The initial concentration of Zn (II) is 500 mg.kg -1 . The composite microbial remediation agent of the invention is added into the soil sample, the addition amount of the composite microbial remediation agent is 2% of the total weight of the soil sample, and the composite microbial remediation agent and the soil sample are uniformly mixed. Water was added to the soil to maintain the soil moisture content at 50% and the soil was treated by shaking at 110rpm at 25 ℃. Centrifuging the soil sample after 3 days, and measuring the final concentrations of Cu (II), fe (III), cd (II), pb (II) and Zn (II) in the soil sample to be 0.93 mg.kg respectively -1 、1.32mg·kg -1 、0.95mg·kg -1 、1.21mg·kg -1 、1.30mg·kg -1 The soil restoration agent provided by the invention has the advantages that the removal rates of Cu (II), fe (III), cd (II), pb (II) and Zn (II) in tea garden soil are respectively 99.69%, 99.66%, 99.77%, 99.66% and 99.74%, and the removal rate of complex heavy metals in 5 mixed heavy metal pollution conditions can be seen to be still very high.
Example 4
The composite microbial remediation agent is used for remediation and treatment of the tea garden heavy metal contaminated soil, and the heavy metal contaminated condition of the tea garden soil to be remediated is simulated as follows:
adding CuSO into the tested soil 4 ·5H 2 O、ZnSO 4 ·7H 2 O、NiCl 2 ·6H 2 O、3CdSO 2 ·8H 2 O、FeCl 3 ·6H 2 O、MnSO 4 ·H 2 O、Pb(NO 3 ) 2 、CrCl 3 ·6H 2 O and Na 2 Cr 2 O 7 ·2H 2 O solution to make initial concentration of Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI) in soil be 500 mg.kg respectively -1 、300mg·kg -1 、400mg·kg -1 、350mg·kg -1 、450mg·kg -1 、550mg·kg -1 、600mg·kg -1 、200mg·kg -1 、350mg·kg -1 . The composite microbial restoration agent is added into the tea garden soil sample, the addition amount of the composite microbial restoration agent is 3 percent of the total weight of the soil sample, and the composite microbial restoration agent and the soil sample are uniformly mixed. Water was added to the soil to maintain the soil moisture content at 50% and the soil was treated by shaking at 150rpm at 25 ℃. Centrifuging the soil sample after 3 days, and measuring the final concentrations of Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI) in the soil sample to be 1.52 mg.kg respectively -1 、0.99mg·kg -1 、0.87mg·kg -1 、1.01mg·kg -1 、1.38mg·kg -1 、1.21mg·kg -1 、0.69mg·kg -1 、1.43mg·kg -1 、1.58mg·kg -1 The soil remediation agent disclosed by the invention has the advantages that the removal rates of Cu (II), zn (II), ni (II), cd (II), fe (III), mn (II), pb (II), cr (III) and Cr (VI) in soil are respectively 99.7%, 99.67%, 99.78%, 99.71%, 99.7%, 99.78%, 99.89%, 99.29 and 99.65%, and the removal rate of heavy metals in orchard soil is not reduced under the condition of pollution of the 9 mixed heavy metals, so that the removal rate is still kept extremely high.
Comparative example 1
According to the method of example 1, when only heavy metal resistant actinomycetes NJ07-19 and nano ferroferric oxide are adopted, the weight ratio is 3:2, when combined, was used for the treatment of the tea garden soil to be remedied in example 2, the results were: the restoration agent has the removal rates of 90.12%, 89.32% and 92.15% for Cu (II), zn (II) and Cd (II) in tea garden soil, and the restoration effect is obviously reduced.
Comparative example 2
According to the method of example 1, when only heavy metal enriched actinomycetes CS-16 and nano ferroferric oxide are adopted, the weight ratio is 1:2, when combined, was used for the treatment of the tea garden soil to be remedied in example 2, the results were: the restoration agent has the removal rates of 92.23%, 90.30% and 91.51% on Cu (II), zn (II) and Cd (II) in tea garden soil respectively, and the restoration effect is obviously reduced.
Comparative example 3
According to the method of example 1, when only heavy metal-resistant actinomycetes NJ07-19 and heavy metal-enriched actinomycetes CS-16 are used, the weight ratio is 3:1, when combined, was used for the treatment of the tea garden soil to be remedied in example 2, the results were: the restoration agent has the removal rates of 95.58 percent, 96.19 percent and 97.42 percent for Cu (II), zn (II) and Cd (II) in the tea garden soil respectively, and the restoration effect is obviously reduced.
Comparative example 4
According to the method of example 1, when heavy metal-resistant actinomycetes NJ07-19, heavy metal-enriched actinomycetes CS-16 and nano ferroferric oxide are adopted, the weight ratio is 3:1:1, when combined, was used for the treatment of the tea garden soil to be remedied in example 3, the results were: the restoration agent has the removal rates of 96.21%, 98.12%, 95.33%, 95.62% and 97.13% on Cu (II), fe (III), cd (II), pb (II) and Zn (II) in tea garden soil, and the restoration effect is reduced compared with the embodiment of the invention.
Claims (6)
1. A method for restoring and treating tea garden polluted soil is characterized in that a microbial restoration agent is applied to tea garden soil to treat heavy metal polluted soil, wherein the microbial restoration agent is prepared from heavy metal-resistant actinomycetesNJ07-19, heavy metal enriched actinomycetes CS-16 and nano ferroferric oxide according to the weight ratio of 3:1:2; wherein, the preservation number of the heavy metal-resistant actinomycetes NJ07-19 is CCTCC No: m2015737; the collection number of the heavy metal enrichment actinomycetes CS-16 is CGMCC No.21594; the concentration of the bacterial liquid of the heavy metal resistant actinomycetes NJ07-19 in the microbial remediation agent is 6.0x10 7 The concentration of bacterial liquid of heavy metal enriched actinomycetes CS-16 is 3.5X10 per mL 7 And each mL.
2. The method according to claim 1, wherein the nano ferroferric oxide has a purity of 99.5% or more and an average particle diameter of 20nm or less.
3. The method according to claim 1 or 2, wherein the two actinomycetes are respectively cultured, the two bacterial solutions are mixed after the required bacterial solution concentration is reached, then nano ferroferric oxide powder is added into the bacterial solutions, and the bacterial solutions are uniformly mixed and then applied into the tea garden soil as a microbial restoration agent.
4. A method according to claim 3 wherein the microbial remediation agent is applied to the tea garden soil in an amount of from 2 to 3% by weight of the total soil.
5. The method according to claim 1 or 2, wherein the heavy metals in the tea garden contaminated soil comprise at least one of Cu (ii), zn (ii), ni (ii), cd (ii), fe (iii), mn (ii), pb (ii), cr (iii), cr (VI).
6. A method according to claim 3, wherein the heavy metals in the tea garden contaminated soil comprise at least one of Cu (ii), zn (ii), ni (ii), cd (ii), fe (iii), mn (ii), pb (ii), cr (iii), cr (VI).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211210104.6A CN115634924B (en) | 2022-09-30 | 2022-09-30 | Tea garden polluted soil restoration and treatment method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211210104.6A CN115634924B (en) | 2022-09-30 | 2022-09-30 | Tea garden polluted soil restoration and treatment method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN115634924A CN115634924A (en) | 2023-01-24 |
CN115634924B true CN115634924B (en) | 2024-04-12 |
Family
ID=84941273
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202211210104.6A Active CN115634924B (en) | 2022-09-30 | 2022-09-30 | Tea garden polluted soil restoration and treatment method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115634924B (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006067085A1 (en) * | 2004-12-20 | 2006-06-29 | Eurovix S.R.L. | Method for decontaminating polluted soil |
CN102199562A (en) * | 2011-03-22 | 2011-09-28 | 四川农业大学 | Composite bacterial preparation as well as preparation method and application thereof |
CN104962294A (en) * | 2015-06-10 | 2015-10-07 | 中山市巴斯德农业科技有限公司 | Soil conditioner and preparation method thereof |
CN105861362A (en) * | 2016-04-13 | 2016-08-17 | 南京北盛荣能源科技有限公司 | Heavy metal resisting actinomycete strain and application thereof |
-
2022
- 2022-09-30 CN CN202211210104.6A patent/CN115634924B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006067085A1 (en) * | 2004-12-20 | 2006-06-29 | Eurovix S.R.L. | Method for decontaminating polluted soil |
CN102199562A (en) * | 2011-03-22 | 2011-09-28 | 四川农业大学 | Composite bacterial preparation as well as preparation method and application thereof |
CN104962294A (en) * | 2015-06-10 | 2015-10-07 | 中山市巴斯德农业科技有限公司 | Soil conditioner and preparation method thereof |
CN105861362A (en) * | 2016-04-13 | 2016-08-17 | 南京北盛荣能源科技有限公司 | Heavy metal resisting actinomycete strain and application thereof |
Non-Patent Citations (3)
Title |
---|
3种有机酸对伴矿景天修复效率及土壤微生物数量的影响;柏佳;谭长银;曹雪莹;周青;黄硕霈;彭曦;邓月强;孙丽娟;;水土保持学报(第02期);全文 * |
复垦矿区重金属对土壤微生物群落的影响;卢永强;陈浮;马静;王辉;张绍良;;环境科学与技术(第03期);全文 * |
茶园污染及控制措施;杨普香;李文金;聂樟清;;蚕桑茶叶通讯(第04期);全文 * |
Also Published As
Publication number | Publication date |
---|---|
CN115634924A (en) | 2023-01-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10131840B2 (en) | Method for preparing iron silicon sulfur multi-element composite biochar soil heavy metal conditioner | |
CN109504398B (en) | Humic acid nano zero-valent iron soil Cr pollution remediation agent and preparation method thereof | |
CN106905980B (en) | Heavy metal soil remediation agent, preparation method and application thereof in sludge digestion | |
Su et al. | Chemical speciation and phytoavailability of Zn, Cu, Ni and Cd in soil amended with fly ash-stabilized sewage sludge | |
EP1968898B1 (en) | Bioremediation materials | |
Liu et al. | Inoculation of Cd-contaminated paddy soil with biochar-supported microbial cell composite: A novel approach to reducing cadmium accumulation in rice grains | |
CN105217802A (en) | A kind of water quality cleansing agent | |
Parameswari et al. | Biosorption and metal tolerance potential of filamentous fungi isolated from metal polluted ecosystem. | |
CN104830740A (en) | Preparation and application methods of efficient microorganism agent for treating compound fertilizer wastewater | |
El-Sheekh et al. | Efficacy of immobilized biomass of the seaweeds Ulva lactuca and Ulva fasciata for cadmium biosorption | |
CN113248093A (en) | Ecological composite bottom mud in-situ restoration agent and restoration process | |
CN115634924B (en) | Tea garden polluted soil restoration and treatment method | |
CN113732052B (en) | Pseudomonas soil remediation agent and application thereof in remediation of heavy metal contaminated soil | |
CN115532817B (en) | Method for repairing and treating heavy metal contaminated soil in orchard | |
CN108570325B (en) | Microbial preparation for high-salinity soil remediation and preparation method thereof | |
Zhang et al. | Evaluation of dissipation mechanisms for pyrene by maize (Zea mays L.) in cadmium co-contaminated soil | |
CN103381418B (en) | Method for processing tobacco waste or organic fluorine wastewater | |
Zhu et al. | Isolation of 2 simazine-degrading bacteria and development of a microbial agent for bioremediation of simazine pollution | |
Zhou et al. | Sorption and biodegradability of sludge bacterial extracellular polymers in soil and their influence on soil copper behavior | |
CN102373155A (en) | Enrichment screening method of polycyclic aromatic hydrocarbons (PAHs) aerobic degrading bacterium | |
Narula et al. | Plant–Microbe Interaction in Heavy-Metal-Contaminated Soils | |
CN108704938B (en) | Compound microbial agent, preparation method thereof and application thereof in high-salinity soil remediation | |
CN114570761B (en) | Method for remediating soil uranium pollution by using biological mineralization of pseudomonas syriacus in Guuriaceae | |
CN111961624B (en) | Composite microbial agent for efficiently degrading nitrogen and phosphorus as well as preparation method and application thereof | |
Saufie et al. | Evaluation of Nutrient Removal Efficiency with Chitosan: Nutrient Composition and Bacterial Removal in Effluents of Nile Tilapia (Oreochromis niloticus) in the Hatchery |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |