CN115634648A - Preparation device and method for producing polysulfated chondroitin sulfate - Google Patents
Preparation device and method for producing polysulfated chondroitin sulfate Download PDFInfo
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- CN115634648A CN115634648A CN202211364992.7A CN202211364992A CN115634648A CN 115634648 A CN115634648 A CN 115634648A CN 202211364992 A CN202211364992 A CN 202211364992A CN 115634648 A CN115634648 A CN 115634648A
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- chondroitin sulfate
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- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Polymers FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 title claims abstract description 131
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 230000007246 mechanism Effects 0.000 claims abstract description 120
- 238000000926 separation method Methods 0.000 claims abstract description 63
- 238000002156 mixing Methods 0.000 claims abstract description 61
- 239000011259 mixed solution Substances 0.000 claims abstract description 54
- 229920001287 Chondroitin sulfate Polymers 0.000 claims abstract description 45
- 229940059329 chondroitin sulfate Drugs 0.000 claims abstract description 36
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Substances NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000008213 purified water Substances 0.000 claims abstract description 20
- 239000002244 precipitate Substances 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 16
- KEQGZUUPPQEDPF-UHFFFAOYSA-N 1,3-dichloro-5,5-dimethylimidazolidine-2,4-dione Chemical compound CC1(C)N(Cl)C(=O)N(Cl)C1=O KEQGZUUPPQEDPF-UHFFFAOYSA-N 0.000 claims abstract description 10
- XTHPWXDJESJLNJ-UHFFFAOYSA-N chlorosulfonic acid Substances OS(Cl)(=O)=O XTHPWXDJESJLNJ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000047 product Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims description 50
- 238000003860 storage Methods 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 4
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Polymers CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 229910021653 sulphate ion Inorganic materials 0.000 claims 1
- 239000012528 membrane Substances 0.000 abstract description 9
- 239000000706 filtrate Substances 0.000 description 21
- 238000007789 sealing Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920002567 Chondroitin Polymers 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Polymers OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- -1 amino polysaccharide Chemical class 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000003042 chondroprotective agent Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
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- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a preparation device and a preparation method for producing polysulfated chondroitin sulfate, and belongs to the technical field of production equipment of the polysulfated chondroitin sulfate, the scheme can realize that in the preparation process of the polysulfated chondroitin sulfate, prepared chondroitin sulfate formamide solution, chlorosulfonic acid, formamide mixed solution, ethanol and purified water are added into a mixing mechanism to be mixed and stirred, so that the polysulfated chondroitin sulfate mixed solution is prepared, then the polysulfated chondroitin sulfate mixed solution is released to flow into a centrifugal separation mechanism through the mixing mechanism, precipitates in the polysulfated chondroitin sulfate mixed solution are separated, so that the polysulfated chondroitin sulfate enters an accommodating mechanism to be collected, finally, 10 times of the mass of dissolved chondroitin sulfate solution is added into the accommodating mechanism by a worker, and the purified chondroitin sulfate is filtered by a 0.45 mu m membrane and then freeze-dried to obtain a freeze-dried product of the polysulfated chondroitin sulfate, and the preparation of the polysulfated chondroitin sulfate is completed.
Description
Technical Field
The invention relates to the technical field of polysulfated chondroitin sulfate production equipment, in particular to a preparation device and a preparation method for producing polysulfated chondroitin sulfate.
Background
With the aging of the world population, the prevalence rate of osteoarthritis and other diseases is increasing, and the population suffering from osteoarthritis and other diseases is increasing, but currently, the common non-steroidal anti-inflammatory drugs, polysulfated acid chondroprotective agents and polysaccharide chondroprotective agents are heparin drugs, such as amino polysaccharide polysulfate, sodium pentose polysulfate, chondroitin polysulfate and the like.
The chondroitin sulfate is a sulfated glycosaminoglycan compound, the molecular weight of a natural product is generally between 10kD and 50kD, and the chondroitin sulfate is widely existed in animal cartilage tissues, and researches show that the chondroitin sulfate has a strong effect of reducing triglyceride and cholesterol in blood and can effectively prevent atherosclerosis, but the preparation method of the chondroitin in the prior art has the problems of complicated preparation process and device and uneven mixing of solutions, so the invention of the preparation device and the method for producing the polysulfated chondroitin sulfate is urgently needed.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to provide a preparation device and a preparation method for producing polysulfated chondroitin sulfate, which can realize that in the preparation process of the polysulfated chondroitin sulfate, firstly, a prepared chondroitin sulfate formamide solution and a mixed solution of chlorosulfonic acid and formamide are added into a mixing mechanism for mixing and stirring, ethanol and purified water are added again after a certain time, the mixture is kept still for a period of time and then stirred, so that a polysulfated chondroitin sulfate mixed solution is prepared, then, the polysulfated chondroitin sulfate mixed solution is released to flow into a centrifugal separation mechanism through the mixing mechanism, the polysulfated chondroitin sulfate mixed solution is centrifugally shaken, precipitates in the polysulfated chondroitin sulfate mixed solution are separated, so that the precipitates are left in the centrifugal separation mechanism, the prepared polysulfated chondroitin sulfate enters the storage mechanism through the centrifugal separation mechanism to finish collection, finally, a worker adds 10 times of the mass of the chondroitin sulfate in the storage mechanism for purification, and the chondroitin sulfate is filtered by a 0.45 mu m film, so that the chondroitin sulfate is dissolved in water and is lyophilized, and the polysulfated chondroitin sulfate is prepared.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
The scheme can realize that in the preparation process of the multi-sulfated chondroitin sulfate, firstly, prepared chondroitin sulfate formamide solution, chlorosulfonic acid and formamide mixed solution are added into the mixing mechanism for mixing and stirring, ethanol and purified water are added again after a certain time, the mixture is stirred after standing for a period of time, so that the multi-sulfated chondroitin sulfate mixed solution is prepared, then, the multi-sulfated chondroitin sulfate mixed solution is released to flow into the centrifugal separation mechanism through the mixing mechanism, the multi-sulfated chondroitin sulfate is centrifugally whipped, precipitates in the chondroitin sulfate are separated, so that the precipitates are left in the centrifugal separation mechanism, the chondroitin sulfate enters the storage mechanism through the centrifugal separation mechanism, the chondroitin sulfate is collected in the storage mechanism, and the quality of the prepared chondroitin sulfate is increased by 45-fold, and the lyophilized chondroitin sulfate is filtered by a freeze-drying mechanism, so that the prepared chondroitin sulfate is dissolved in 0.0 mu m.
Further, the mixing mechanism comprises a mixing box fixedly connected between the inner walls of the supporting frames, a plurality of uniformly distributed conveying pipes are embedded at the upper end of the mixing box, a conveying pump is installed at the outer end of each conveying pipe, a driving rotating shaft is installed at the top end in the mixing box and electrically connected with a servo motor, a plurality of uniformly distributed first rotating grooves are formed in the outer end of the driving rotating shaft, stirring rods are rotatably connected between the inner walls of the first rotating grooves and electrically connected with the servo motor, a hydrophobic coating is coated on the inner wall of the mixing box, a second rotating groove is formed in the lower end of the mixing box, a sealing plate is rotatably connected between the inner walls of the second rotating grooves, a control switch is installed at the rotating shaft of the sealing plate and the second rotating groove, and in the process of preparing polysulfated chondroitin sulfate, the conveying pump is started firstly, adding 10-23% chondroitin sulfate formamide solution, chlorosulfonic acid and formamide mixed solution into a mixing box through solutions corresponding to different conveying pipes, then starting a servo motor to drive a rotating shaft to rotate and drive a stirring rod to stir the mixed solution, wherein the stirring rod drives the rotating shaft to do up-and-down swinging operation in a first rotating tank under the action of electrical connection of the servo motor during stirring, so that the stirring rod does circular motion and up-and-down movement in the mixing box, thereby greatly improving the mixing efficiency between the mixed solutions, then drives the rotating shaft and the stirring rod to stop operating after a certain time, and injects ethanol and purified water into the mixing box again to stir after standing for a period of time, thereby preparing polysulfided chondroitin sulfate mixed solution, after the mixing and stirring work is finished, the control switch is controlled to enable the sealing plate to rotate, so that the prepared polysulfated chondroitin sulfate mixed liquid is released downwards.
Furthermore, the stirring rod comprises a built-in cavity which is formed inside, a supporting plate is fixedly connected between the left inner wall and the right inner wall of the built-in cavity, a plurality of uniformly distributed extension springs are fixedly connected to the upper end and the lower end of the supporting plate, a plurality of marbles are arranged on the upper side and the lower side of the supporting plate, and when the stirring rod swings in the circumferential direction and the up-and-down direction to stir, the plurality of marbles inside the stirring rod continuously impact the extension springs under the action of shaking, so that the stirring rod is impacted again under the action of the elastic force of the extension springs, and the polysulfated chondroitin sulfate mixed liquid adhered to the surface of the stirring rod is vibrated and dropped into the mixing box under the action of shock generated when the marbles impact the inner wall of the stirring rod, so that the polysulfated chondroitin sulfate mixed liquid is prevented from having residues on the surface of the stirring rod, and the cleaning work of later-period workers is reduced.
Further, stop gear includes fixed connection's between the support frame inner wall limiting base, the bottom end chisel has the through-hole in the limiting base, four limiting support are installed to limiting base inner wall, four limiting support all is diagonal distribution, and the contained angle between the just is 90.
Further, spacing support includes four support bases of spacing base inner wall fixed connection, the annular spout is dug to support base outer end, sliding connection has the gag lever post between the annular spout inner wall, when centrifugal separation mechanism changes and joins in marriage to spacing base inner wall, the staff causes it to shrink to inside the annular spout through pressing down the gag lever post to it slides to support base below to lead to centrifugal separation mechanism to pass through the size of support base, and the staff takes spacing support out once more afterwards, thereby carries on spacingly with the centrifugal separation mechanism of below this moment, prevents to appear the phenomenon of skew when the stop mechanism inner wall is placed.
Further, the centrifugal separation mechanism comprises a separation base connected with the inner wall of the limiting mechanism in a sliding manner, four limiting grooves are formed in the outer end of the separation base, the four limiting grooves are distributed diagonally and form a 90-degree included angle, the diameter size of each limiting groove is identical to that of the support base, filtrate holes are formed in the inner bottom end of the separation base, a driving base is arranged between the inner walls of the filtrate holes, a filtrate film is fixedly connected between the driving base and the inner walls of the filtrate holes, a plugging mechanism is installed between the inner walls of the separation base, a centrifugal storage tank is fixedly connected to the upper end of the driving base, a filtrate film is embedded in the outer end of the centrifugal storage tank, a threaded cover plate is connected between the inner walls of the centrifugal storage tank in a threaded manner, a filter screen is embedded in the upper end of the threaded cover plate, and the centrifugal storage tank and the threaded cover plate are both located on the lower side of the plugging mechanism, when a worker assembles the centrifugal separation mechanism, the worker can mutually correspond the position of the limiting groove and the limiting bracket and then slide to the lower surface of the limiting bracket, before the prepared mixed solution of the poly-sulfated chondroitin sulfate is released to the centrifugal separation mechanism, the worker can control to open the plugging mechanism to enable the plugging mechanism to contract, the prepared mixed solution of the poly-sulfated chondroitin sulfate enters the centrifugal storage tank through the filter screen, then the plugging mechanism is started again to enable the plugging mechanism to wholly seal the separation base, then the driving base is started to enable the centrifugal storage tank to carry out centrifugal separation work, precipitates in the prepared mixed solution of the poly-sulfated chondroitin sulfate are filtered, the poly-sulfated chondroitin sulfate enters the space between the separation base and the inner wall of the centrifugal storage tank through the filtrate membrane and then flows into the accommodating mechanism through the filtrate hole, the completion is to the preparation of polysulfated chondroitin sulfate to after the preparation of polysulfated chondroitin sulfate was accomplished, the staff in the later stage, the accessible rotates the thread cover board, with the thread cover board from the inside roll-out of centrifugal bin, then will separate the whole taking out of base, thereby clear up the deposit of centrifugal bin inside.
Further, the plugging mechanism comprises a storage support fixedly connected between the inner walls of the separation bases, folding plates are mounted between the upper inner wall and the lower inner wall of the storage support, the folding plates and the storage support are in fan-shaped telescopic shapes, when workers control the folding plates to perform closing work, the folding plates are in fan-shaped telescopic shapes and are completely stored between the inner walls of the storage supports, and polysulfated chondroitin sulfate enters the centrifugal storage box.
Further, the containing mechanism comprises a containing base fixedly connected with the inner bottom end of the supporting frame, an annular hole is formed in the upper end of the containing base, the diameter of the annular hole is matched with that of the filtrate hole, the annular hole is located on the lower side of the filtrate hole, a collecting box is connected between the inner walls of the containing base in a sliding mode, a handle is fixedly connected to the outer end of the collecting box, when polysulfated chondroitin sulfate entering the space between the separating base and the inner wall of the centrifugal storage box through centrifugal separation works flows downwards through the filtrate film, the polysulfated chondroitin sulfate enters the inside of the collecting box through the annular hole, collection of the polysulfated chondroitin sulfate is completed, purified water with 10 times of chondroitin sulfate feeding mass can be added into the inside of the collecting box to be dissolved, and the purified water is filtered through a 0.45 mu m film and then is freeze-dried, and a freeze-dried product of the polysulfated chondroitin sulfate is obtained.
A method for preparing a device for producing polysulfated chondroitin sulfate comprises the following steps:
s1, injecting a prepared chondroitin sulfate formamide solution and a mixed solution of chlorosulfonic acid and formamide into a mixing mechanism for mixing and stirring, and injecting ethanol and purified water again after a certain time to stir after standing for a period of time, so as to prepare a polysulfated chondroitin sulfate mixed solution;
s2, releasing the mixed solution of the polysulfated chondroitin sulfate to flow into a centrifugal separation mechanism through a mixing mechanism, carrying out centrifugal swing on the mixed solution of the polysulfated chondroitin sulfate, and separating precipitates in the mixed solution of the polysulfated chondroitin sulfate;
s3, the prepared oversulfated chondroitin sulfate enters the storage mechanism through the centrifugal separation mechanism to be collected, purified water with the mass being 10 times that of the added chondroitin sulfate is added into the storage mechanism by a worker to be dissolved, and the solution is filtered by a 0.45 mu m membrane and then is freeze-dried to obtain a oversulfated chondroitin sulfate freeze-dried product, so that the preparation of the oversulfated chondroitin sulfate is completed.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
according to the scheme, in the preparation process of the multi-sulfated chondroitin sulfate, firstly, the mixed chondroitin sulfate formamide solution and the mixed solution of chlorosulfonic acid and formamide are added into a mixing mechanism for mixing and stirring, ethanol and purified water are added again after a certain time, the mixture is allowed to stand for a period of time and then is stirred, so that the multi-sulfated chondroitin sulfate mixed solution is prepared, then the multi-sulfated chondroitin sulfate mixed solution is released to flow into a centrifugal separation mechanism through the mixing mechanism, the multi-sulfated chondroitin sulfate mixed solution is centrifugally shaken, precipitates in the multi-sulfated chondroitin sulfate mixed solution are separated, the precipitates are left in the centrifugal separation mechanism, the prepared multi-sulfated chondroitin sulfate enters an accommodating mechanism through the centrifugal separation mechanism to be collected, finally, purified water with the mass being 10 times that of the chondroitin sulfate is added into the accommodating mechanism by a worker, and the precipitates are filtered by a 0.45 mu m film and then are freeze-dried to obtain the multi-sulfated chondroitin sulfate freeze-dried product, and the preparation of the multi-sulfated chondroitin sulfate is completed.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the mixing mechanism of the present invention;
FIG. 3 is a schematic view of the stirring rod of the present invention;
FIG. 4 is a schematic structural view of a spacing mechanism of the present invention;
FIG. 5 is a schematic view of the structure of the position-limiting bracket of the present invention;
FIG. 6 is a schematic view of the centrifugal separation mechanism of the present invention;
FIG. 7 is a schematic exploded view of the centrifugal separation mechanism of the present invention;
FIG. 8 is a schematic exploded view of the centrifugal storage bin of the present invention;
FIG. 9 is a schematic structural view of the plugging mechanism before and after expansion;
fig. 10 is a schematic structural view of the receiving mechanism of the present invention.
The reference numbers in the figures illustrate:
100 support frames, 200 mixing mechanisms, 201 mixing boxes, 202 conveying pipes, 203 conveying pumps, 204 driving rotating shafts, 205 first rotating grooves, 206 stirring rods, 2061 built-in cavities, 2062 supporting plates, 2063 extension springs, 2064 marble, 207 hydrophobic coatings, 208 second rotating grooves, 209 sealing plates, 210 control switches, 300 servo motors, 400 limiting mechanisms, 401 limiting bases, 402 through holes, 403 limiting supports, 4031 support bases, 4032 annular sliding grooves, 4033 limiting rods, 500 centrifugal separation mechanisms, 501 separation bases, 502 limiting grooves, 503 filtrate holes, 504 driving bases, 505 filtrate membranes, 506 plugging mechanisms, 5061 storage supports, 5062 folding plates, 507 centrifugal storage boxes, 508 filtrate membranes, 509 threaded cover plates, 510 filter screens, 600 storage mechanisms, 601 storage bases, 602 annular holes, 603 collection boxes and 604 handles.
Detailed Description
The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", etc. indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, which are merely for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "fitted/connected", "connected", and the like, are to be interpreted broadly, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The embodiment is as follows:
referring to fig. 1-10, a device for preparing polysulfated chondroitin sulfate comprises a support 100, a mixing mechanism 200 is fixedly connected between inner walls of the support 100, a servo motor 300 is installed at an outer end of the support 100, a limiting mechanism 400 is installed between inner walls of the support 100, the limiting mechanism 400 is located at a lower side of the mixing mechanism 200, a centrifugal separation mechanism 500 is arranged inside the limiting mechanism 400, a receiving mechanism 600 is installed at an inner bottom end of the support 100, and the receiving mechanism 600 is located at a lower side of the limiting mechanism 400, so that in the process of preparing the polysulfated chondroitin sulfate, firstly, the prepared chondroitin sulfate formamide solution, the chlorosulfonic acid and formamide mixed solution are added into the mixing mechanism 200 for mixing and stirring, and then, ethanol and purified water are added again after a certain time, stirring after standing for a period of time, thereby preparing a mixed solution of polysulfated chondroitin sulfate, subsequently releasing the mixed solution of polysulfated chondroitin sulfate to flow into the centrifugal separation mechanism 500 through the mixing mechanism 200, centrifugally shaking the mixed solution of polysulfated chondroitin sulfate, separating precipitates in the mixed solution of polysulfated chondroitin sulfate, so that the precipitates are left in the centrifugal separation mechanism 500, allowing the prepared polysulfated chondroitin sulfate to enter the storage mechanism 600 through the centrifugal separation mechanism 500 to complete collection, finally adding purified water with 10 times of the feeding mass of the chondroitin sulfate into the storage mechanism 600 by a worker, dissolving the purified water with 0.45 mu m of membrane filtration, and freeze-drying to obtain a freeze-dried product of the polysulfated chondroitin sulfate, thereby completing the preparation of the polysulfated chondroitin sulfate.
Referring to fig. 1-2, the mixing mechanism 200 comprises a mixing box 201 fixedly connected between inner walls of a support frame 100, a plurality of uniformly distributed feed delivery pipes 202 are embedded at the upper end of the mixing box 201, a feed delivery pump 203 is installed at the outer end of the feed delivery pipes 202, a driving rotating shaft 204 is installed at the top end of the mixing box 201, the driving rotating shaft 204 is electrically connected with a servo motor 300, a plurality of uniformly distributed first rotating grooves 205 are drilled at the outer end of the driving rotating shaft 204, a stirring rod 206 is rotatably connected between the inner walls of the first rotating grooves 205, the stirring rod 206 is electrically connected with the servo motor 300, a hydrophobic coating 207 is coated on the inner wall of the mixing box 201, a second rotating groove 208 is drilled at the lower end of the mixing box 201, a sealing plate 209 is rotatably connected between the inner walls of the second rotating groove 208, a control switch 210 is installed at the rotating shaft of the sealing plate 209 and the second rotating groove 208, in the process of preparing polysulfated sulfuric acid, firstly, a material conveying pump 203 is started, chondroitin sulfate formamide solution with the concentration of 10 to 23 percent by mass and mixed solution of chlorosulfonic acid and formamide are filled into a mixing box 201 through solutions corresponding to different material conveying pipes 202, then a servo motor 300 is started, so that a driving rotating shaft 204 rotates to drive a stirring rod 206 to stir the mixed solution, and the stirring rod 206 performs up-and-down swinging operation in a first rotating groove 205 under the action of the electrical connection of the servo motor 300 for a certain time in the stirring process, so that the stirring rod 206 performs circular motion and up-and-down movement in the mixing box 201, thereby greatly improving the mixing efficiency of the mixed solution, then the rotating shaft 204 and the stirring rod 206 are driven to stop operation after a certain time, ethanol and purified water are filled into the mixing box 201 again, and stirring is performed after standing for a period of time, after the mixing and stirring work is finished, the control switch 210 is controlled to enable the sealing plate 209 to rotate, so that the prepared mixed solution of the polysulfated chondroitin sulfate is released downwards.
Referring to fig. 2-3, the stirring rod 206 includes an internal cavity 2061 drilled therein, a supporting plate 2062 is fixedly connected between the left and right inner walls of the internal cavity 2061, a plurality of uniformly distributed tension springs 2063 are fixedly connected to the upper and lower ends of the supporting plate 2062, and a plurality of marbles 2064 are disposed on the upper and lower sides of the supporting plate 2062, when the stirring rod 206 is in the circumferential direction and in the process of up-and-down swinging stirring, the plurality of marbles 2064 therein continuously impact the tension springs 2063 under the action of shaking, so that the stirring rod 206 is impacted again under the action of the elastic force of the tension springs 2063, and the mixed solution of polysulfated chondroitin sulfate adhered to the surface of the stirring rod 206 is vibrated and dropped into the mixing box 201 under the action of the shaking force generated by the marbles 2064 impacting the inner wall of the stirring rod 206, thereby preventing the mixed solution of polysulfated chondroitin sulfate from remaining on the surface of the stirring rod 206 and reducing the cleaning work of the workers in the later period.
Referring to fig. 1 and 4, the limiting mechanism 400 includes a limiting base 401 fixedly connected to the inner wall of the supporting frame 100, a through hole 402 is drilled at the bottom end of the limiting base 401, four limiting brackets 403 are installed on the inner wall of the limiting base 401, the four limiting brackets 403 are diagonally distributed, and the included angle therebetween is 90 °.
Referring to fig. 4-5, the limiting bracket 403 includes four bracket bases 4031 fixedly connected to the inner walls of the limiting base 401, an annular sliding groove 4032 is cut at the outer end of the bracket base 4031, and a limiting rod 4033 is slidably connected between the inner walls of the annular sliding groove 4032, when the centrifugal separation mechanism 500 is rotatably coupled to the inner wall of the limiting base 401, a worker presses the limiting rod 4033 to contract the limiting rod 4032 into the annular sliding groove 4032, so that the centrifugal separation mechanism 500 can slide below the bracket base 4031 by the size of the bracket base 4031, and then the worker withdraws the limiting bracket 403 again, thereby limiting the centrifugal separation mechanism 500 below the limiting bracket at this time, and preventing the inner wall of the limiting mechanism 400 from being displaced when placed.
Referring to fig. 1 and fig. 6-8, the centrifugal separation mechanism 500 comprises a separation base 501 slidably connected to the inner wall of a limiting mechanism 400, four limiting grooves 502 are cut on the outer end of the separation base 501, the four limiting grooves 502 are distributed diagonally and form an included angle of 90 degrees, the diameter of the limiting grooves 502 is matched with the diameter of the bracket base 4031, a filtrate hole 503 is cut on the inner bottom end of the separation base 501, a driving base 504 is arranged between the inner walls of the filtrate hole 503, a filtrate film 505 is fixedly connected between the driving base 504 and the inner wall of the filtrate hole 503, a blocking mechanism 506 is arranged between the inner walls of the separation base 501, a centrifugal storage box 507 is fixedly connected to the upper end of the driving base 504, a filtrate film 508 is embedded on the outer end of the centrifugal storage box 507, a threaded cover plate 509 is threadedly connected between the inner walls of the centrifugal storage box 507, a filter screen 510 is embedded on the upper end of the threaded cover plate 509, the centrifugal storage box 507 and the threaded cover plate 509 are both located on the lower side of the blocking mechanism 506, when a worker assembles the centrifugal separation mechanism 500, the worker can slide to the lower surface of the limiting bracket 403 after the limiting groove 502 and the limiting bracket 403 correspond to each other, then before the prepared mixed solution of the polysulfated chondroitin sulfate is released to the centrifugal separation mechanism 500, the worker can control to open the plugging mechanism 506 to cause the plugging mechanism 506 to contract, the prepared mixed solution of the polysulfated chondroitin sulfate enters the centrifugal storage tank 507 through the filter screen 510, then the plugging mechanism 506 is started again to cause the plugging mechanism 506 to seal the whole separation base 501, then the driving base 504 is started to cause the centrifugal storage tank 507 to carry out centrifugal separation work, precipitates in the prepared mixed solution of the polysulfated chondroitin sulfate are filtered, and the mixed solution of the polysulfated chondroitin sulfate enters between the separation base 501 and the inner wall of the centrifugal storage tank 507 through the filter liquid membrane 508, then flow in penetrating filtering liquid hole 503 to receiving mechanism 600, accomplish the preparation to polysulfated chondroitin sulfate to after polysulfated chondroitin sulfate preparation was accomplished, the staff in the later stage, accessible rotation thread cover plate 509, with thread cover plate 509 from the inside roll-out of centrifugal storage case 507, then will separate the whole of base 501 and take out, thereby clear up the inside precipitate of centrifugal storage case 507.
Referring to fig. 1, 6 and 9, the blocking mechanism 506 includes a storage bracket 5061 fixedly connected between inner walls of the separation base 501, a folding plate 5062 is installed between upper and lower inner walls of the storage bracket 5061, the folding plate 5062 and the storage bracket 5061 are extended and retracted in a fan shape, when a worker controls the folding plate 5062 to perform a closing operation, the folding plate 5062 is retracted in a fan shape until being completely stored between inner walls of the storage bracket 5061, so that the polysulfated chondroitin sulfate enters the inside of the centrifugal storage box 507, when the worker controls the folding plate 5062 to perform an opening and closing operation, the folding plate 5062 is extended in a fan shape, and then the separation base 501 is closed, thereby preventing the polysulfated chondroitin sulfate from being splashed out of the separation base 501 during the centrifugal separation operation and causing waste.
Referring to fig. 1 and 10, the receiving mechanism 600 includes a receiving base 601 fixedly connected to the inner bottom end of the supporting frame 100, an annular hole 602 formed in the upper end of the receiving base 601, the diameter of the annular hole 602 matches the diameter of the filtrate hole 503, the annular hole 602 is located on the lower side of the filtrate hole 503, a collecting box 603 is slidably connected between the inner walls of the receiving base 601, a handle 604 is fixedly connected to the outer end of the collecting box 603, when the polysulfated chondroitin sulfate entering between the separating base 501 and the inner wall of the centrifugal storage box 507 flows downward through the filtrate membrane 505 through centrifugal separation, the polysulfated chondroitin sulfate enters the collecting box 603 through the annular hole 602, the collection of the polysulfated chondroitin sulfate is completed, then, purified water with 10 times of the mass of the chondroitin sulfate can be added into the collecting box 603 for dissolving, and the chondroitin sulfate is filtered by a 0.45 μm membrane and then lyophilized to obtain a freeze-dried product of the polysulfated chondroitin sulfate.
A method for preparing a device for producing polysulfated chondroitin sulfate comprises the following steps:
s1, adding a prepared chondroitin sulfate formamide solution and a mixed solution of chlorosulfonic acid and formamide into a mixing mechanism 200 for mixing and stirring, adding ethanol and purified water again after a certain time, and stirring after standing for a period of time to prepare a polysulfated chondroitin sulfate mixed solution;
s2, releasing the mixed solution of the polysulfurized chondroitin sulfate to flow into the centrifugal separation mechanism 500 through the mixing mechanism 200, carrying out centrifugal swing on the mixed solution of the polysulfurized chondroitin sulfate, and separating precipitates in the mixed solution of the polysulfurized chondroitin sulfate;
s3, enabling the prepared oversulfated chondroitin sulfate to penetrate through the centrifugal separation mechanism 500 to enter the containing mechanism 600 to finish collection, adding 10 times of purified water of the chondroitin sulfate feeding mass into the containing mechanism 600 by a worker for dissolving, filtering by a 0.45 mu m membrane, and then freeze-drying to obtain a freeze-dried oversulfated chondroitin sulfate product, thereby finishing the preparation of the oversulfated chondroitin sulfate.
The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.
Claims (9)
1. A preparation facilities of polysulfated chondroitin sulfate production usefulness, includes support frame (100), its characterized in that: fixedly connected with mixing mechanism (200) between support frame (100) inner wall, servo motor (300) are installed to support frame (100) outer end, install stop gear (400) between support frame (100) inner wall, stop gear (400) are located mixing mechanism (200) downside, stop gear (400) inside is equipped with centrifugal separation mechanism (500), receiving mechanism (600) are installed to the bottom in support frame (100), receiving mechanism (600) are located stop gear (400) downside.
2. The apparatus of claim 1, wherein the apparatus comprises: mixing mechanism (200) is including fixed connection's mixing box (201) between support frame (100) inner wall, mixing box (201) upper end inlays conveying pipeline (202) that are equipped with a plurality of evenly distributed, conveying pump (203) are installed to conveying pipeline (202) outer end, drive pivot (204) are installed to the top in mixing box (201), electric connection between drive pivot (204) and servo motor (300), drive pivot (204) outer end is excavated first rotation groove (205) that have a plurality of evenly distributed, it is connected with stirring rod (206) to rotate between first rotation groove (205) inner wall, electric connection between stirring rod (206) and servo motor (300), mixing box (201) inner wall is scribbled and is equipped with hydrophobic coating (207), mixing box (201) lower extreme is excavated there is second rotation groove (208), rotate between second rotation groove (208) inner wall and be connected with closing plate (209), closing plate (209) and second rotation groove (208) pivot department install control switch (210).
3. The apparatus of claim 2, wherein the apparatus comprises: the stirring rod (206) comprises a built-in cavity (2061) which is drilled inside, a supporting plate (2062) is fixedly connected between the left inner wall and the right inner wall of the built-in cavity (2061), a plurality of uniformly distributed extension springs (2063) are fixedly connected to the upper end and the lower end of the supporting plate (2062), and a plurality of marbles (2064) are arranged on the upper side and the lower side of the supporting plate (2062).
4. The apparatus of claim 1, wherein the apparatus comprises: stop gear (400) include fixed connection's between support frame (100) inner wall limiting base (401), it has through-hole (402) to open the chisel in limiting base (401) bottom end, four limiting support (403), four are installed to limiting base (401) inner wall limiting support (403) all are diagonal distribution, and the contained angle between the just is 90.
5. The apparatus of claim 4, wherein the apparatus comprises: spacing support (403) include four support bases (4031) of spacing base (401) inner wall fixed connection, annular spout (4032) are dug to support base (4031) outer end, sliding connection has gag lever post (4033) between annular spout (4032) the inner wall.
6. The apparatus of claim 1, wherein the apparatus comprises: centrifugal separation mechanism (500) is including stop gear (400) inner wall sliding connection's separation base (501), four spacing groove (502) have been dug to separation base (501) outer end, four spacing groove (502) all are diagonal distribution, and contained angle between them is 90, the diameter size of spacing groove (502) is identical with the diameter size of support base (4031), it has filtrating hole (503) to open the chisel in separation base (501) inner bottom, be equipped with drive base (504) between filtrating hole (503) inner wall, fixedly connected with filtrating film (505) between drive base (504) and filtrating hole (503) inner wall, install shutoff mechanism (506) between separation base (501) inner wall, drive base (504) upper end fixedly connected with centrifugal storage case (507), centrifugal storage case (507) outer end inlays and is equipped with filtrating film (508), threaded connection has threaded cover board (509) between centrifugal storage case (507) inner wall, threaded cover board (509) upper end inlays and is equipped with filter screen (510), centrifugal cover board (507), threaded cover board (509) downside all is located shutoff mechanism (506).
7. The apparatus of claim 6, wherein: plugging mechanism (506) are including separating frame (501) between the inner wall fixed connection accomodate support (5061), accomodate and install folding plate (5062) between the upper and lower inner wall of support (5061), folding plate (5062) and accomodate and be fan-shaped form flexible between support (5061).
8. The apparatus for the production of polysulfated chondroitin sulphate of claims 1 and 6, wherein: receiving mechanism (600) include support frame (100) inner bottom end fixed connection's accomodate base (601) upper end and open annular hole (602), the diameter size of annular hole (602) and the diameter size phase-match of filtrating hole (503), annular hole (602) are located the downside of filtrating hole (503), sliding connection has collection box (603) between accomodating base (601) inner wall, collection box (603) outer end fixedly connected with handle (604).
9. The method for manufacturing a device for the production of polysulfated chondroitin sulfate according to any of claims 1 to 8, wherein: the method comprises the following steps:
s1, injecting a prepared chondroitin sulfate formamide solution and a mixed solution of chlorosulfonic acid and formamide into a mixing mechanism (200) for mixing and stirring, injecting ethanol and purified water again after a certain time, and stirring after standing for a period of time to prepare a polysulfated chondroitin sulfate mixed solution;
s2, releasing the mixed solution of the polysulfated chondroitin sulfate to flow into a centrifugal separation mechanism (500) through a mixing mechanism (200), carrying out centrifugal swing on the mixed solution of the polysulfated chondroitin sulfate, and separating precipitates in the mixed solution of the polysulfated chondroitin sulfate;
s3, the prepared oversulfated chondroitin sulfate penetrates through the centrifugal separation mechanism (500) to enter the storage mechanism (600) to be collected, purified water with the mass being 10 times that of the chondroitin sulfate is added into the storage mechanism (600) by a worker for dissolving, and the purified water is subjected to freeze-drying after membrane filtration by 0.45 mu m to obtain a oversulfated chondroitin sulfate freeze-dried product, so that the preparation of the oversulfated chondroitin sulfate is completed.
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| CN202211364992.7A CN115634648A (en) | 2022-11-03 | 2022-11-03 | Preparation device and method for producing polysulfated chondroitin sulfate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116678185A (en) * | 2023-06-08 | 2023-09-01 | 嘉兴恒杰生物制药股份有限公司 | Production method and equipment of sodium chondroitin sulfate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116678185A (en) * | 2023-06-08 | 2023-09-01 | 嘉兴恒杰生物制药股份有限公司 | Production method and equipment of sodium chondroitin sulfate |
| CN116678185B (en) * | 2023-06-08 | 2023-12-12 | 嘉兴恒杰生物制药股份有限公司 | Production method and equipment of sodium chondroitin sulfate |
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Application publication date: 20230124 |