CN115633736A - Feed additive for improving growth of spotted-brown broiler chickens, chicken feed and preparation method and application of chicken feed - Google Patents

Feed additive for improving growth of spotted-brown broiler chickens, chicken feed and preparation method and application of chicken feed Download PDF

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CN115633736A
CN115633736A CN202211326095.7A CN202211326095A CN115633736A CN 115633736 A CN115633736 A CN 115633736A CN 202211326095 A CN202211326095 A CN 202211326095A CN 115633736 A CN115633736 A CN 115633736A
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spotted
brown
growth
feed additive
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谢小玲
罗凯文
周建平
韦建权
黄永建
杨胜杰
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Guangzhou Kevin Food Co ltd
Guangdong Kaiwen Biological Technology Co ltd
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Guangdong Kaiwen Biological Technology Co ltd
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Abstract

The invention discloses a feed additive for improving the growth of spotted-brown broiler chickens, a chicken feed, and a preparation method and application thereof, and belongs to the technical field of feeds. The feed additive comprises the following components in parts by weight: 30-50 parts of lauric glyceride, 20-40 parts of butyrin, 20-40 parts of assistant and 1-10 parts of organic acid. According to the feed additive for improving the growth of the ephedra broiler chicken, the lauric acid glyceride and the butyric acid glyceride are introduced, the unique action mechanism of different carbon chain fatty acid glycerides is comprehensively utilized, and the effects of the lauric acid glyceride and the butyric acid glyceride are improved. Improve the digestion and absorption of the feed, promote the growth and weight increment, enhance the immune system and regulate the intestinal health.

Description

Feed additive for improving growth of spotted-brown broiler chickens, chicken feed and preparation method and application of chicken feed
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a feed additive for improving the growth of spotted-brown broiler chickens, a chicken feed, and a preparation method and application thereof.
Background
At present, under the conditions of scale, intensification and high density, chicks are easily stimulated by various stress factors, the disease resistance of the body is weak, and the chicks are more easily threatened by various bacterial and viral diseases, so that a series of problems of growth obstruction, immunity inhibition, intestinal dysfunction and the like are caused, and the breeding benefit is seriously influenced. Because of the health degree of the chick culture stage, the subsequent breeding is greatly influenced. In the past, antibiotics and some medicines are often relied on, and the use of antibiotics and medicines may be harmful to human health. Therefore, the research and development of nonreactive technology and the guarantee of smooth culture are urgent.
In recent years, the drug resistance of special antibacterial drugs for animals is increased year by abusing antibiotics, which affects food safety and public health safety. In the antibiotic age after banning resistance, substitute products of antibiotics are actively searched, the culture gap is filled, and the method has potential huge economic benefit market. The existing market has a lot of antibiotics, including Chinese herbal medicines, acidifiers, enzyme preparations, essential oil, antibacterial peptide and the like, which have various characteristics but have some defects.
The alpha-glycerol monolaurate is a novel feed additive, has multiple functions of emulsifying property, bacteriostasis and virus resistance, regulating lipid metabolism, enhancing immunity, regulating intestinal flora structure and the like, and has no corrosivity, bad smell and taste. The feed additive can emulsify and disperse grease droplets in the feed into fine oil droplets, increase the contact area of lipase and the fine oil droplets and promote the digestion and absorption of fat; the fat envelope virus inhibitor has obvious inhibiting effect on fat envelope viruses such as blue ear diseases and the like in livestock and poultry; improving the lipid metabolism of the liver of an animal body, and slowing down or even treating the aggravation of the fatty liver; enhancing the immune system of the animal body and promoting the proliferation and differentiation of T lymphocytes; promoting the proliferation of beneficial bacteria in intestinal tract and inhibiting the proliferation of harmful bacteria. Is an ideal antibiotic substitute product and has wide development prospect.
The butyrin has good regulating effect on the intestinal health of animals. After being ingested by animals, the feed is not decomposed in the stomach, and after reaching the intestinal tract, the feed is decomposed into butyric acid and butyrin through the action of enzymes in the intestinal tract: (1) The energy is rapidly supplied to the intestinal epithelial cells, the growth of the small intestinal mucosa cells is effectively promoted, the maintenance and the repair of the intestinal mucosa are participated, and the integrity and the function of the intestinal mucosa cells are maintained; (2) The villus height of the intestinal tract of the animal is increased, the crypt depth is reduced, the ratio of the intestinal villus height to the crypt depth is increased, and the small intestine morphological structure is improved; (3) Reducing the pH value of intestinal tracts, inhibiting the growth of pathogenic bacteria such as escherichia coli, salmonella, clostridium perfringens and the like, promoting the proliferation of beneficial bacteria of lactic acid bacteria, and regulating the intestinal tract microecological balance of livestock and poultry; (4) Promoting the secretion of antibodies and immune cell proliferation of intestinal mucosa, enhancing the anti-stress and immunity capability of livestock and poultry, and reducing intestinal inflammation and other diseases.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a feed additive for improving the growth of spotted-brown broiler chickens. The product has the advantages of good safety, reliability, cost performance and the like, the short-chain fatty acid ester product is compounded and used, the respective characteristics are comprehensively utilized, the short-chain fatty acid ester product generates interaction through a reasonable preparation scheme, the efficacy of the short-chain fatty acid ester product is improved, the antibacterial drug resistance can not be generated in animal bodies, and the use of antibiotics is effectively replaced.
The invention discloses a feed additive for improving the growth of spotted-brown broiler chickens, which comprises the following components in parts by weight:
30-50 parts of lauric glyceride, 20-40 parts of butyrin, 20-40 parts of silicon dioxide and 1-10 parts of compound acidifier.
In some embodiments of the invention, the following components are included in parts by weight:
40 parts of lauric glyceride, 30 parts of butyrin, 30 parts of silicon dioxide and 5 parts of compound acidifier.
In some embodiments of the invention, the glycerol monolaurate is primarily alpha-glycerol monolaurate.
In some embodiments of the invention, the glyceryl butyrate compositions are glyceryl monobutyrate, glyceryl dibutyrate and glyceryl tributyrate.
In some embodiments of the invention, the complex acidulant includes at least one of citric acid and fumaric acid.
In some embodiments of the invention, the mass ratio of the citric acid to the fumaric acid in the compound acidulant is (50-80) to (20-50).
In some embodiments of the invention, the mass ratio of citric acid to fumaric acid in the complex acidulant is 80:20.
The second aspect of the invention discloses a preparation method of the feed additive for improving the growth of spotted-brown broiler chicken, which is characterized by comprising the following steps:
s01, mixing the raw materials in proportion;
s02, spraying powder and granulating to obtain the solid granular feed additive.
The third aspect of the invention discloses a chicken feed comprising the feed additive for improving the growth of the spotted-brown broiler chickens, and preferably, the weight content of the feed additive for improving the growth of the spotted-brown broiler chickens is 0.01-1%.
The fourth aspect of the invention discloses application of the feed additive for improving the growth of spotted-brown broiler chickens in the first aspect in preparation of chicken feed.
The invention has the beneficial effects that:
1. the lauric acid glyceride and the butyric acid glyceride are introduced, the unique action mechanism of different carbon chain fatty acid glycerides is comprehensively utilized, and the effects of the lauric acid glyceride and the butyric acid glyceride are improved. Improve the digestion and absorption of the feed, promote the growth and weight increment, enhance the immune system and regulate the intestinal health.
2. The silicon dioxide is used as a carrier, and is subjected to solid granulation treatment by a spray granulation process. The high surface activity, the microporous structure and the large specific surface area of the silicon dioxide are utilized to efficiently load, the good biocompatibility and the gastrointestinal tract mucosa affinity of the silicon dioxide are kept, and the silicon dioxide has controlled and sustained release effects on the loaded materials.
3. The compound acidifier can improve the palatability of the feed, increase the appetite and increase the feed intake; the pH value in the stomach can be reduced, and the activity of pepsin is accelerated, so that the digestion and absorption of protein are promoted; can also improve gastrointestinal microflora, promote the reproduction and growth of beneficial bacteria and microorganisms such as lactobacillus, and inhibit the reproduction of harmful bacteria such as Escherichia coli and salmonella.
4. Test results show that the growth performance of the spotted-brown broiler chickens is remarkably improved during the high-density breeding period by adding the feed additive; reducing liver index, and maintaining liver health; increases the level of zonulin ZO-1 and occludin; improves species abundance, stability and beneficial bacteria level of intestinal flora, and promotes intestinal health.
Drawings
FIG. 1 shows the average end weight of 21d of spotted-brown broiler chickens treated by different dosages of alpha-GML;
FIG. 2 shows the 21d intestinal length of spotted-brown broiler chickens treated by different dosages of alpha-GML;
FIG. 3 is the liver index of spotted-brown broiler chickens treated by different dosages of alpha-GML;
FIG. 4 is thymus organ index of broiler chickens treated by different dosages of alpha-GML;
FIG. 5 is spleen organ index of broiler chickens treated with different dosages of alpha-GML;
FIG. 6 shows the protein expression of ZO-1, occludin in chicken intestinal tissues of each group;
FIG. 7 shows the expression levels of ZO-1 proteins in each group;
FIG. 8 shows the expression levels of occludin proteins in each group;
FIG. 9 is a plot of relative abundance accumulation for genus-level species;
FIG. 10 is a Venn diagram between treatment groups;
FIG. 11 shows the results of the structural analysis of the caecum flora, the structural analysis of the caecum flora and the analysis of the taxonomic composition of the genus level;
FIG. 12 is a graph of the level abundance of each set of samples;
fig. 13 is an alpha-diversity index, a.shannon index, b.simpson index, c.chao index and d.ace index;
FIG. 14 is a hierarchical clustering tree for each set of samples.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps, preparation parameters.
Raw materials: lauric acid glyceride (Kaiwen food development Co., ltd., guangzhou city, alpha-monolaurin content is more than or equal to 85%); butyrin (Weifang Keneng Biotechnology limited, mono-, di-and tributyrin > 50%); silica (Shanxi Tond chemical Co., ltd.); citric acid (Jungbunzlauer); fumaric acid (Zibovisus speciality chemical Co., ltd.). The compound acidulant comprises citric acid and fumaric acid, and the mass ratio of the citric acid to the fumaric acid is 80.
Examples
The feed additive for improving the growth of the spotted-brown broiler chicken adopts a powder spraying and granulating process and is prepared by mixing the raw materials in parts by weight in the following table into a solid granular feed additive.
Lauric acid glyceride Butyric acid glyceride Silicon dioxide () Compound acidifier (star)
Example 1 30 40 20 1
Example 2 50 20 40 10
Example 3 40 30 30 5
Example 4 35 35 30 7
Example 5 40 40 20 (corn gluten meal) 5
Practice ofExample 6 40 40 20 (Bean pulp) 5
Example 7 43 35 24 6 (citric acid)
Example 8 43 35 24 6 (fumaric acid)
Example 9 43 35 24 6 (tartaric acid)
Comparative example 1 0 70 20 1
Comparative example 2 70 0 20 1
Note: the unit of each datum is weight portion; note that: * Where specified, are the specified components, and are otherwise silica; note: * Where specifically indicated, are indicated components, otherwise are complex acidulants.
EXAMPLE 1 (Overall examination)
1200 1 day old spotted brown broiler chicks were selected and randomly divided into 4 treatment groups of 6 replicates each, with 50 replicates each. The control group was fed with basal diet, and the test group was fed with 0.05%, 0.1%, and 0.2% of the feed additive of example 1, respectively, based on the basal diet. The basic diet is a commercial product.
Specific test groups are shown in the following table:
TABLE 1 test grouping
Figure BDA0003912099900000061
The high-density cage-rearing chicken house is adopted for breeding, and during the experiment, a specially-assigned person is responsible for breeding according to the conventional breeding operation rules and immunizing according to the conventional immunization program.
After 21 days of cultivation, 30 chickens were randomly selected for each group for sample treatment and data collection, and experimental data were analyzed and processed.
Influence of 1 alpha-GML treatment for 21d on growth performance of spotted-brown broiler chicken
FIG. 1 shows the average end weight of 21d of spotted-brown broiler chickens treated by different dosages of alpha-GML. The test results are expressed as "mean ± standard deviation". "" indicates the degree of difference from the corresponding control (.: P <0.05,: P < 0.01), and the lower graph indicates the same method.
From the above figure, it can be seen that at 1-21d, compared with the blank control group, the average end weight of the alpha-GML group added with 500mg/kg of the basic ration is slightly increased but the difference is not significant, while the average end weight of the ephedra broilers is significantly increased by the alpha-GML group added with 1000mg/kg and 2000mg/kg of the basic ration, wherein the average end weight of the alpha-GML group added with 1000mg/kg of the basic ration is increased by 11.85% (p < 0.01), and the average end weight of the alpha-GML group added with 2000mg/kg of the basic ration is increased by 9.45% (p < 0.01). Compared with the group of 500mg/kg, the group added with 1000mg/kg of basal diet and 2000mg/kg of alpha-GML has 10.3 percent (p < 0.01) and 7.9 percent (p < 0.01) of average terminal weight, and the difference is very obvious. The results show that the addition of 1000mg/kg of alpha-GML and 2000mg/kg of alpha-GML in the basic daily ration can remarkably improve the growth performance of the spotted-brown broiler chickens during 1-21d of high-density culture.
Influence of 2 alpha-GML treatment for 21d on intestinal length and liver index of ephedra broiler chicken
Fig. 2 shows the 21d intestinal length of the spotted-brown broiler chickens treated by different dosages of alpha-GML, and fig. 3 shows the liver index of the spotted-brown broiler chickens treated by different dosages of alpha-GML.
As can be seen from FIG. 2I, at 1-21d, compared with the blank control group, the intestinal lengths of the alpha-GML groups added with 500mg/kg,1000mg/kg and 2000mg/kg of basal diet are slightly increased but the difference is not significant. Figure 3 shows that the liver index of the blank control group is similar to that of the alpha-GML group added with 500mg/kg (p > 0.05), while the liver index of the group added with 1000mg/kg,2000mg/kg alpha-GML is lower than that of the blank control group (p < 0.01), and the difference is very significant. The result shows that the addition of the alpha-GML in the daily ration hardly influences the intestinal length of the spotted-brown broiler, but the liver indexes of the alpha-GML group of 1000mg/kg and 2000mg/kg are remarkably reduced.
Influence of 3 alpha-GML treatment for 21d on immune organ index of spotted-brown broiler chicken
The figure is thymus organ index of the broiler chickens treated by different dosages of alpha-GML, and the figure 5 is spleen organ index of the broiler chickens treated by different dosages of alpha-GML.
The effect of the feed additive on the immune organ index of the spotted-brown broiler is shown in figure 4.
As shown in FIG. 4, the thymus organ index of the group with 1000mg/kg and 2000mg/kg of alpha-GML added to the diet was higher than that of the blank control group and that of the group with 500mg/kg of alpha-GML added to the diet (p > 0.05), and the difference was not significant. FIG. 5 shows that spleen organ index became large (p > 0.05) with no significant difference as the amount of daily ration added in the daily ration increased. The result shows that the addition of the alpha-GML in the daily ration can improve the immune organ index to a certain extent to enhance the immune function of the ephedra broiler chicken.
Influence of 4 alpha-GML treatment on serum biochemical indexes of the spotted-brown chickens by 21d on the influence of the feed additive on the serum biochemical indexes of the spotted-brown chickens, and the serum biochemical index results are shown in a table 2.
TABLE 2 Effect on serum biochemical indices of spotted-brown chicken
Figure BDA0003912099900000071
Figure BDA0003912099900000081
Note: * Indicating significant difference compared with the control group, p <0.05; * Indicates a very significant difference compared to the control group, p <0.01.
Compared with a control group, the activity of the lactate dehydrogenase added into the diet of the group with 1000mg/kg of alpha-GML is obviously improved by 55.4 percent (p is less than 0.05). Compared with the control group, the serum glutamic pyruvic transaminase activity is remarkably reduced by 23.1% (p is less than 0.05) and 21.4% (p is less than 0.05) by adding 500mg/kg and 2000mg/kg of alpha-GML in the diet respectively, and the serum glutamic pyruvic transaminase activity is remarkably reduced by 31.0% (p is less than 0.01) by adding 1000mg/kg of alpha-GML in the diet. The other indexes have no significant difference. Glutamate pyruvate transaminase is one of the most characteristic functional indexes of animal livers. When the liver is damaged by various factors, the hepatocytes cannot maintain their structural integrity and glutamate pyruvate transaminase permeates into the blood. Thus, increased glutamate pyruvate transaminase activity is a response to liver damage, and the detection of increased glutamate pyruvate transaminase activity in serum may indicate a disruption in the normal function of the liver. Experimental results show that the activity of serum glutamic pyruvic transaminase can be remarkably reduced by adding GML into the diet, which indicates that the liver function of the chicken is improved.
Influence of 5 alpha-GML treatment on 21d on Ephedra chicken intestinal proteins ZO-1 and occludin
FIG. 6 shows the protein expression of ZO-1,occludin in each group of chicken intestinal tissues. The corresponding relation between each group name and the addition amount of the alpha-GML is as follows: blank-0 mg/kg; low-500 mg/kg; medium-1000 mg/kg; the height is-2000 mg/kg. The same is applied below.
FIG. 7 shows the ZO-1 protein expression levels of each group, and FIG. 8 shows the occludin protein expression levels of each group.
The results of Western Blot analysis of the effect of alpha-GML treatment for 21d on the Ephedra chicken intestinal tight junction protein ZO-1, occludin are shown in 6,7,8. Analysis of the results shows that after the alpha-GML treatment is carried out for 21 days, the content of the ephedra broiler intestinal tight junction protein ZO-1 is increased and a certain dosage effect is presented. The low group has a significant increase in ZO-1 content (p < 0.05) over the blank group; the middle group has extremely obviously increased ZO-1 content (p < 0.01) compared with the blank group; the middle group, the high group, also had a very significant increase in ZO-1 content (p < 0.01) compared to the low group. The results show that after the alpha-GML treatment for 21 days, the content of the ephedra broiler intestinal tight junction protein occludin is also increased, and the difference is significant. Wherein the occludin content is significantly increased in the low and high groups compared to the blank group (p < 0.05); the occludin content was significantly increased in the middle group compared to the blank group (p < 0.01). The results show that after the alpha-GML is treated for 21 days, the content of ZO-1,occludin in intestinal tract of the ephedra broiler chicken is increased, and the difference is significant. .
7 influence of feed additive on caecum flora of ephedra broilers
FIG. 9 is a plot of relative abundance accumulation for genus-level species; as shown in the figure, the late trend of the accumulation curve of species in each genus level is flat, which indicates that the sampling quantity is sufficient, and the data analysis can be carried out.
FIG. 10 is a Venn diagram between treatment groups; as can be seen, a total of 290 OTUs were obtained for the four treatment groups, and 130 OUT were common for each treatment group, with 3 OTUs unique to the control group, 14 OUT unique to the low dose group, 6 OUT unique to the medium dose group, and 14 OUT unique to the high dose group.
FIG. 11 shows the results of structural analysis of the cecal flora, including structural analysis of the cecal flora and analysis of the taxonomic composition of the genus level; as can be seen from the figure, at the phylum level, the sum of 2 phyla, namely Bacteroidetes (Bacteroides) and Firmicutes (Firmicutes), reaches more than 92%. The control group was most abundant in Firmicutes and the α -GML treated group was most abundant in Bacteroidetes. The content of cecropin (bacteroides) in the control group is obviously lower than that in the low dose group (p < 0.05), the medium dose group (p < 0.05) and the high dose group (p < 0.05). As can be seen from the figure, at the genus level, the control group had significantly lower levels of Papanicolaria (Barnesiella) than those of the low dose group (p < 0.05), the medium dose group (p < 0.05), and the high dose group (p < 0.05), and significantly higher levels of Ruminococcaceae-UCG-014 than those of the low dose group (p < 0.05), the medium dose group (p < 0.05), and the high dose group (p < 0.05). Consistent with the gate level, the Bacteroides (Bacteroides) content of the control group was also significantly lower than that of the low dose group (p < 0.05), the medium dose group (p < 0.05), and the high dose group (p < 0.05).
FIGS. 12-14 are identity analyses, FIGS. 12-13 are alpha-diversity analyses, and FIG. 14 is beta-diversity analyses; wherein, fig. 12 is a grade abundance curve of each group of samples, and the species uniformity and species abundance of the control group, the low dose group and the high dose group are different according to the gentle degree of the later period of the curve and the range of the curve on the horizontal axis. FIG. 13 is an α -diversity index showing that there is a difference in α -diversity between the experimental group and the control group. The Shannon index of the control group is significantly lower than that of the low dose group (p < 0.05) and that of the high dose group (p < 0.05), indicating that the species uniformity of the control group is significantly lower than that of the low dose group and that of the high dose group. The control group had a significantly lower Chao index and ACE index than the low dose group (p < 0.05), indicating that the low dose group had a greater number of species than the control group. FIG. 14 is a hierarchical clustering tree of samples in each group, from which it can be seen that the low dose group, the high dose group are closest and then the medium dose group are close, the three groups are farthest from the control group. The differences of species compositions of the control group, the low dose group, the medium dose group and the high dose group are illustrated.
According to the analysis of the results, the alpha-GML treatment for 21d has great influence on the caecum of the ephedra broilers. The addition of alpha-GML in the daily ration increases the species abundance of the caecum, and the species diversity is also improved, thereby increasing the stability of the flora. According to phylum horizontal taxonomic composition analysis, the addition of alpha-GML reduces the ratio of firmicutes to bacteroidetes of the caecal flora, and according to previous researches, the increase of the ratio of firmicutes to bacteroides is not beneficial to the health of intestinal flora. Therefore, the addition of α -GML is beneficial for the growth of chickens. Bacteroides are significantly increased at the genus level, helping to break down food and produce the nutrients and energy required by the body. It also provides a degree of protection to the gut from invasive pathogens. Therefore, the alpha-GML treatment for 21d has a positive effect on the caecum of the ephedra broilers, and is beneficial to the intestinal health of the chickens.
To summarize: the alpha-GML can remarkably improve the growth performance of the spotted-brown broiler chickens during 1-21d of higher density culture period; reducing liver index, and maintaining liver health; increasing the level of zonulin ZO-1 and Occludin; improves species abundance, stability and beneficial bacteria level of intestinal flora, and promotes intestinal health.
Experimental example 2 (influence of different examples and comparative examples on growth performance of spotted-brown chickens)
The feed additives of examples and comparative examples were added to the feed of the basal diet in a weight ratio of 0.1% to prepare a chicken feed.
The influence of the feed additive on the growth performance of the spotted-brown broiler chickens was examined according to the method of experimental example 1, and the results are shown in table 3.
TABLE 3 influence on the growth Performance of Ephedra broilers
21d average last seed (g)
Example 1 315
Example 2 309
Example 3 327
Example 4 304
Example 5 297
Example 6 290
Example 7 306
Example 8 310
Example 9 294
Comparative example 1 286
Comparative example 2 289
The results show that the feed additives of examples 1-9 have obvious effect (p < 0.01) on the growth performance of the spotted-brown chickens, and are superior to those of comparative examples 1 and 2, wherein the feed additive of example 1 is optimal (p < 0.01). The feed additive for improving the growth of the spotted-brown broiler chicken introduces the lauric acid glyceride and the butyric acid glyceride, comprehensively utilizes the unique action mechanism of different carbon chain fatty acid glycerides, and improves the effects of the lauric acid glyceride and the butyric acid glyceride.
While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the embodiments and examples, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims (10)

1. The feed additive for improving the growth of the spotted-brown broiler chicken is characterized by comprising the following components in parts by weight:
30-50 parts of lauric glyceride, 20-40 parts of butyrin, 20-40 parts of assistant and 1-10 parts of organic acid.
2. The feed additive for improving growth of spotted-brown broiler chickens according to claim 1, is characterized by comprising the following components in parts by weight:
40 parts of lauric glyceride, 30 parts of butyrin, 30 parts of auxiliary agent and 5 parts of organic acid.
3. The feed additive for improving growth of spotted-brown broiler chickens of claim 1 or 2, wherein the glycerol monolaurate is α -glycerol monolaurate as a main component.
4. The feed additive for improving growth of spotted-brown broiler chickens of claims 1-3, wherein said butyrin ester component is glycerol monobutyrate, glycerol dibutyrate and glycerol tributyrate.
5. The feed additive for improving growth of spotted-brown broiler chickens of any one of claims 1-4, wherein said additive is selected from at least one of silicon dioxide, corn protein powder and bean meal.
6. The feed additive for improving the growth of spotted-brown broiler chickens of claims 1-5, wherein said organic acid is selected from at least one of citric acid, fumaric acid and tartaric acid; preferably a complex acidulant of citric and fumaric acids.
7. The feed additive for improving the growth of spotted-brown broiler chickens according to any one of claims 1-6, wherein the mass ratio of citric acid to fumaric acid in the compound acidulant is (70-90) to (10-30), preferably 80:20.
8. The preparation method of the feed additive for improving the growth of spotted-brown broiler chickens according to any one of claims 1-7, characterized by comprising the following steps:
s01, mixing the raw materials in proportion;
s02, spraying powder and granulating to obtain the solid granular feed additive.
9. A chicken feed comprising the feed additive for improving the growth of spotted-brown broiler chickens according to any one of claims 1-8, preferably the weight content of the feed additive for improving the growth of spotted-brown broiler chickens is 0.01-1%.
10. The application of the feed additive for improving the growth of spotted-brown broiler chickens according to any one of claims 1-7 in the preparation of chicken feed.
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CN105724821A (en) * 2016-03-04 2016-07-06 江苏省家禽科学研究所 Chicken feed composite acidifier as well as preparation method and application thereof
CN105916385A (en) * 2013-11-20 2016-08-31 普罗维隆控股股份有限公司 Animal feed comprising a combination of mono glycerides
CN112006165A (en) * 2019-05-30 2020-12-01 龙岩新奥生物科技有限公司 Emulsion composition, preparation method and application thereof

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Publication number Priority date Publication date Assignee Title
CN105916385A (en) * 2013-11-20 2016-08-31 普罗维隆控股股份有限公司 Animal feed comprising a combination of mono glycerides
CN105724821A (en) * 2016-03-04 2016-07-06 江苏省家禽科学研究所 Chicken feed composite acidifier as well as preparation method and application thereof
CN112006165A (en) * 2019-05-30 2020-12-01 龙岩新奥生物科技有限公司 Emulsion composition, preparation method and application thereof

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Application publication date: 20230124