CN115629468A - Optical imaging system - Google Patents

Abstract

The present application provides an optical imaging system, comprising: the system comprises a laser, a laser adapter and a microscope host; the laser is used for emitting laser to the laser adapter; the laser adapter is used for receiving laser emitted by the laser, adjusting and adapting the laser, and then transmitting the adjusted and adapted laser to the microscope host; the microscope host is arranged to transmit laser to the microscope probe and control the microscope probe to perform laser scanning on a living body to generate a fluorescence signal for imaging. In the technical scheme that this application provided, the laser that the laser instrument produced passes through the laser adapter, and the laser adapter converts the laser signal of the various differences of receiving into unified laser signal output, even the position of changing laser instrument or laser instrument produced a pair ofly like this, the homoenergetic is so that laser and follow-up connected equipment adaptation.

Description

Optical imaging system

Technical Field

The application relates to the technical field of optics, in particular to an optical imaging system.

Background

Direct recording of neuronal activity in freely moving living animals is one of the most direct and effective methods for studying the relationship between animal behavior and neurological function. The multiphoton optical imaging system is the most important and widely used tool for neuron observation due to its good optical slicing capability and deep penetration depth. The multi-photon optical imaging system can be a two-photon, three-photon, raman and other nonlinear laser scanning microscope equipment.

Among traditional many photon optical imaging system, the mode that laser instrument and optical adjusting bracket were fixed on optical platform carries out the regulation of light path, and the light path enters into microscope host computer through the speculum after the plastic, because the light path from between laser instrument to the microscope host computer is space light path, therefore the microscope host computer also must be stable fix on optical platform to guarantee that the light path of main part inside can not receive external force and take place to deflect, thereby influence microscopical performance.

However, since many modules, such as a beam shaping module, a circuit control module, various drivers, a fluorescence collection module, a wide-field fluorescence module, a laser module, etc., are usually disposed on the periphery, the apparatus is complicated, the wiring is complicated, and the modules are easily interfered by signals and are easily operated by human beings, so that the light path is easily deflected.

In addition, the light path and the microscope host are fixed, and experiments with position and direction requirements on the microscope main body cannot be changed or realized, for example, the laser light path and the microscope main body are not on a platform, even in a room, and the traditional mode cannot be realized. If the laser needs to be replaced or the distance between the lasers is changed, all the optical paths need to be readjusted due to the change of the laser emitted by the lasers, and some lasers cannot be adapted even due to the large parameter difference of the lasers.

Disclosure of Invention

In view of this, the present application provides an optical imaging system to solve the problems of the conventional optical imaging system that the light path needs to be readjusted due to the change of the laser emitted by the laser, and the flexibility of use is poor.

The present application provides an optical imaging system, comprising: the system comprises a laser, a laser adapter and a microscope host; wherein, the first and the second end of the pipe are connected with each other,

the laser is used for emitting laser to the laser adapter;

the laser adapter is used for receiving the laser emitted by the laser, adjusting and adapting the laser, and then transmitting the adjusted and adapted laser to the microscope host;

the microscope host is used for transmitting laser to the microscope probe and controlling the microscope probe to scan the living body with the laser to generate a fluorescence signal.

Optionally, the optical imaging system further includes a first transmission optical fiber connected between the laser adapter and the microscope host, and the laser adapter transmits the adjusted and adapted laser to the microscope host through the first transmission optical fiber.

Optionally, the microscope host includes a laser coupling module, a laser input end of the laser coupling module is connected to the first transmission optical fiber, and a laser output end of the laser coupling module is connected to the microscope probe through a second transmission optical fiber;

the laser coupling module is used for adjusting the laser received from the first transmission optical fiber and then transmitting the laser to the microscope probe through the second transmission optical fiber.

Optionally, the laser adapter comprises a first power detection device for detecting laser power;

the laser coupling module comprises a second power detection device for detecting laser power.

Optionally, the first power detection device is close to a laser output end of the laser adapter, and the second power detection device is close to a laser input end of the laser coupling module.

Optionally, the laser adapter comprises an adapter housing, and a beam transformation device and a first beam stabilization device located in the adapter housing; wherein the content of the first and second substances,

the light beam conversion device is used for carrying out light beam conversion on the laser entering the laser adapter;

the first light beam stabilizing device is arranged at the downstream of the light beam changing device along the laser transmission direction and is used for adjusting the laser transmission direction so as to correct the deviation of the actual position and the ideal position of the laser beam at the laser output end of the laser adapter.

Optionally, the laser coupling module includes a coupler housing, a dispersion compensation element, an acousto-optic modulator, and a second beam stabilizing device, where the dispersion compensation element, the acousto-optic modulator, and the second beam stabilizing device are all disposed in the coupler housing and sequentially disposed along a transmission direction of laser light; wherein the content of the first and second substances,

the dispersion compensation element is used for compensating negative dispersion caused by the transmission optical fiber in the process of transmitting laser light;

the acousto-optic modulator is used for adjusting the intensity of laser;

the second light beam stabilizing device is used for adjusting the laser transmission direction so as to correct the deviation of the actual position and the ideal position of the laser beam at the laser output end of the laser coupling module.

Optionally, the microscope host further comprises a fluorescence acquisition module and a scanning control module;

the scanning control module is connected with the microscope probe through a control cable and is used for controlling the microscope probe to carry out laser scanning so as to generate a fluorescence signal;

the fluorescence collection module is connected with the microscope probe through a fluorescence collection optical fiber and used for collecting the fluorescence signal output by the microscope probe through the fluorescence collection optical fiber.

Optionally, the microscope host further comprises a wide field search module;

the wide field searching module is arranged for performing wide field imaging on a living body so as to search a target area for mounting the microscope probe on the living body.

Optionally, the optical imaging system further comprises a workbench, wherein the workbench comprises a workbench host and a display;

the workbench host is connected with the microscope host, the microscope host processes the collected fluorescence signals and then transmits the processed fluorescence signals to the workbench host, and the display displays images;

the workbench host also sends a control instruction to the microscope host.

In the technical scheme that this application provided, the laser that the laser instrument produced passes through the laser adapter, and the laser adapter can enlarge, reduce and zoom the transform such as to laser beam, converts the laser signal of the various differences of receiving into unified laser signal output for the equipment adaptation of laser and follow-up connection does benefit to the best performance that reaches the system. Thus, lasers with different parameters can be used, or even if the distance of the lasers changes, the received laser can be converted through the laser adapter, and then the laser beam can be output to the microscope host.

Additional features and advantages of the present application will be described in detail in the detailed description which follows.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application. In the drawings:

FIG. 1 is a schematic diagram of an optical imaging system in accordance with an embodiment of the present application;

FIG. 2 is a schematic illustration of an optical path of an optical imaging system in accordance with an embodiment of the present application;

FIG. 3 is a schematic diagram of a laser adapter in an embodiment in accordance with the present application;

FIG. 4 is a schematic diagram of the internal structure of the laser adapter shown in FIG. 3;

FIG. 5 is a schematic diagram of a laser coupling module according to an embodiment of the present application;

fig. 6 is a schematic diagram of an internal structure of the laser coupling module shown in fig. 5;

FIG. 7 is a schematic diagram of a microscope mainframe according to an embodiment of the present application;

FIG. 8 is a schematic structural view of the main unit of the microscope shown in FIG. 7 with the light-shielding door in an open state;

FIG. 9 is a schematic view of the microscope host shown in FIG. 8 in an exploded state;

FIG. 10 is a schematic view of the cooperative mounting of the motion module, living body mount, and visual field searching adapter according to one embodiment of the present application;

FIG. 11 is a schematic diagram of a wide field search module mounted on a laser coupling module in accordance with an embodiment of the present application;

FIG. 12 is a schematic diagram of a control box according to an embodiment of the present application;

FIG. 13 is a front view of the control box of FIG. 12;

fig. 14 is a schematic structural diagram of a receiving device according to an embodiment of the present application.

Description of reference numerals:

100-a laser; 200-a laser adapter; 201-an adapter housing; 202-a first fixed mirror; 203-a second fixed mirror; 204-a light beam transformation device; 205-a first deflection mirror; 206-a second deflection mirror; 207-position detector; 208-a first power detection device; 209-first beam splitter; 210-a switching device; 211-laser input; 212-laser output; 213-support legs; 214-a laser coupler; 300-microscope host; 1-mounting a main body; 11-a base; 12-a mounting frame; 13-a support plate; 2-a shading door; 3-a laser coupling module; 31-laser input end; 32-laser output end; 33-a coupler housing; 311-a second power detection device; 321-a second beam splitter; 331-a light path through hole; 34-a dispersion compensating element; 35-a mirror; 36-an acousto-optic modulator; 361-a driver; 362-heat dissipation fins; 363-a fan; 37-a first deflection mirror; 38-a second deflection mirror; 39-position detector; 4-a visual field searching module; 41-a fluorescent light source; 42-a camera; 43-objective lens; 5-a control box; 51-a first interface; 52-a second interface; 53-a fluorescence collection module; 54-a master control circuit board; 6-a storage device; 61-a containing body; 611-a winding drum; 612-wire blocking disk; 613-wire clamping grooves; 62-probe holder; 63-a protective cover; 631-a viewing window; 7-a mobile module; 8-a living body installation device; 9-visual field search adapter; 91-a probe mounting assembly; 400-a microscope probe; 401-a second transmission fiber; 402-fluorescence collection fiber; 403-control cables; 500-first transmission fiber.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application. In the present application, the embodiments and the features of the embodiments may be combined with each other without conflict.

In the description of the present application, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "axial," "radial," "circumferential," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present application. Further, "inner and outer" refer to inner and outer relative to the contour of each member itself.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature.

The present application provides an optical imaging system, as shown in fig. 1, comprising: a laser 100, a laser adapter 200, and a microscope host 300; wherein the content of the first and second substances,

the laser 100 is used for emitting laser to the laser adapter 200;

the laser adapter 200 is configured to receive laser emitted by the laser 100, adjust and adapt the laser, and transmit the adjusted and adapted laser to the microscope host 300;

the microscope main body 300 is configured to transmit laser light to the microscope probe 400, and controls the microscope probe 400 to perform laser scanning on a living body to generate a fluorescence signal for imaging.

In the technical scheme that this application provided, the laser that laser instrument 100 produced passes through laser adapter 200, and laser adapter 200 can enlarge, reduce and zoom transform such as to laser beam, converts the various different laser signal that receive into unified laser signal output for laser and the equipment adaptation of follow-up connection do benefit to the best performance that reaches the system. In this way, lasers having various parameters can be used, and even if the distance between the lasers changes, the laser adapter 200 converts the received laser beam and outputs the adapted laser beam to the microscope 300.

The optical imaging system provided by the present application may be a multi-photon imaging system, i.e., the microscope probe 400 may employ two-photon, three-photon, raman, and other non-linear laser scanning imaging. In some embodiments, the microscope probe 400 may specifically include a Micro-Electro-Mechanical System (MEMS) scanning galvanometer and various lenses.

In one embodiment, the optical imaging system further includes a first transmission fiber 500 connected between the laser adapter 200 and the microscope host 300, and the laser adapter 200 transmits the adjusted and adapted laser light to the microscope host 300 through the first transmission fiber 500. One end of the first transmission fiber 500 may be connected to a laser coupler, and connected to the output end of the laser adapter 200 through the laser coupler, and the other end is connected to a collimator, and connected to the microscope host 300 through the collimator.

The laser adapter 200 and the microscope host 300 are connected by an optical fiber, so that the microscope host 300 can move freely, and therefore the microscope host 300 can be placed at different positions as required, even placed across platforms, and is more flexible to use.

And the optical fiber output can play a role in shaping the light beam, so that light spots output from the laser adapter 200 to the microscope host 300 are more uniform, and the system performance is favorably improved. In addition, compared with the mode of arranging a fixed light path adjusting device between the laser adapter 200 and the microscope host 300, the mode of optical fiber connection has the advantages that the interference and misoperation are reduced, the stability of the system is improved, the arrangement of the light path adjusting device before the module can be reduced, and the installation and the maintenance are easy.

In one embodiment, the microscope mainframe 300 includes a laser coupling module 3, a laser input end 31 of the laser coupling module 3 is connected with a first transmission optical fiber 500, and a laser output end 32 is connected with the microscope probe 400 through a second transmission optical fiber 401.

The laser coupling module 3 is configured to adjust the laser light received from the first transmission fiber 500 and transmit the adjusted laser light to the microscope probe 400 through the second transmission fiber 401. For example, the laser coupling module 3 may perform dispersion compensation and/or intensity adjustment on the laser light.

In one embodiment, as shown in fig. 2, the laser adapter 200 includes a first power detection device 208 for detecting the laser power, and the laser coupling module 3 includes a second power detection device 311 for detecting the laser power.

The first power detection device 208 can detect the laser power entering the laser adapter 200 in real time, and the power change detected by the first power detection device 208 can detect whether the laser transmission is abnormal, generally, whether the laser 100 is damaged or whether the laser is blocked or the like is determined. Specifically, a first beam splitter 209 may be disposed on the laser transmission path, a part of the laser beam is split by the first beam splitter 209 to the first power detection device 208, and the first power detection device 208 obtains the power of the laser by detecting the split light. The second power detection device 311 may detect the power of the laser in the laser coupling module 3 in real time, and similarly, by obtaining the split light of the laser beam, the second power detection device 311 detects the split light to obtain the laser power. By comparing the power changes of the second power detection device 311 and the first power detection device 208, it can be determined whether the laser transmission between the laser adapter 200 and the microscope host 300 is abnormal, and therefore, the first power detection device 208 and the second power detection device 311 can be used for quickly positioning links with problems in the laser transmission process.

Optionally, the first power detection device 208 is close to the laser output end 212 of the laser adapter 200, and the second power detection device 311 is close to the laser input end 31 of the laser coupling module 3, and if the power change of the second power detection device 311 compared with the first power detection device 208 is large, it may be determined that the first transmission optical fiber 500 has a problem, so that a problem link is quickly located, and quick maintenance may be realized.

In one embodiment, the laser adapter 200 is configured as shown in fig. 3 and 4, and includes an adapter housing 201, and a beam transforming device 204 and a first beam stabilizing device located within the adapter housing 201.

The light beam transformation device 204 is configured to transform the laser light entering the laser adapter 200, and the light beam transformation device 4 may adopt an existing device that can expand or reduce the cross section of the laser beam and can zoom the laser beam, and a specific structure thereof can be implemented by those skilled in the art, and is not described herein again.

The first beam stabilizing device is disposed downstream of the beam varying device 204 along the laser transmission direction, and is used for adjusting the transmission direction of the laser to correct the deviation of the actual position of the laser beam at the laser output end 212 from the ideal position, i.e. controlling the laser to be able to output within a range having a smaller deviation from the ideal output position, so that the laser is stably coupled into the first transmission fiber 500, and the coupling efficiency of the laser output is ensured.

When the lasers 100 with different parameters are adopted and the lasers 100 with different parameters are emitted into the laser adapter 200, the light beams with fixed spot sizes can be uniformly output after being converted by the light beam conversion device 4, so that the lasers output by the different lasers 100 can be adapted to equipment connected at the back; alternatively, when the distance of the laser 100 is changed, the beam converter 204 may perform the zoom process to output a uniform laser beam. When the environment changes (for example, temperature, humidity, or the like changes, or vibration occurs), each device (for example, a laser, a reflecting mirror, a beam splitter, or the like) on the optical path may be vibrated or may be shifted due to the influence of temperature, so that the output direction of the laser changes, and the first beam stabilizing device may adjust the deflection direction of the laser in real time according to the deviation between the actual position and the ideal position of the beam at the laser output end 212, so that the laser is stably output, and the coupling efficiency of the laser output is ensured.

Therefore, with the laser adapter 200 provided in the present application, under the condition that lasers with different parameters are input or deflection occurs during laser transmission, the optical path does not need to be readjusted or an optical device on the optical path needs to be replaced, and the laser adapter 200 can adapt and couple the lasers to a device connected later by transforming the input lasers and adjusting the laser transmission direction.

In one embodiment, as shown in FIG. 4, the first beam stabilization device may include a position detector 207, at least one deflection mirror, and a mirror adjustment mechanism coupled to each deflection mirror;

the position detector 207 is disposed at a position close to the laser output end 212, and configured to detect position information of the laser at the laser output end 212, specifically, a spectroscope may be disposed on the laser transmission path, and a part of the light may be reflected to the position detector 207 by splitting light, so that the position detector 207 detects the position information of the laser at the laser output end 212 according to the light splitting. The position detector 207 may be a 4D position detector, and the 4D position detector can strictly detect the position drift and the angle drift of the light beam, and can accurately detect the real-time position of the light beam. The mirror adjusting mechanism is configured to drive the deflection mirror to deflect according to the position information detected by the position detector 207 so as to adjust the laser transmission direction, so that the laser is stably output.

Specifically, a desired location of the laser beam at the laser output end 212 is first determined, which is a location where the laser can achieve a desired coupling efficiency when the laser is outcoupled into a delivery fiber connected at the laser output end 212. When a light beam deflects, for example, the light beam deflects due to the deviation of an optical device caused by vibration or temperature change, or the light beam deflects due to artificial touch, the position detector 207 detects the position information of the laser at the laser output end 212 in real time and sends the position information to the control unit, the control unit continuously determines the deviation between the position of the laser beam and an ideal position according to the position information, and controls the mirror adjusting mechanism to adjust the position of the deflection mirror, so that the reflection direction of the laser is adjusted, and the laser is stably transmitted in a certain range around the ideal position.

The laser adapter 200 further includes at least one fixed mirror for changing the laser light transmission direction, which is disposed upstream of the beam transformation device 204 in the laser light transmission direction. The fixed reflector is arranged to change the laser transmission direction, so that the light path can be bent, the arrangement of all components on the light path is facilitated, and the reduction of the size of the whole laser adapter is facilitated.

In the embodiment shown in fig. 4, the at least one fixed mirror comprises a first fixed mirror 202 and a second fixed mirror 203, and the at least one deflecting mirror comprises a first deflecting mirror 205 and a second deflecting mirror 206. Alternatively, the incident angle and the exit angle of the laser light at the first fixed mirror 202 and the incident angle and the exit angle at the second fixed mirror 203 are respectively substantially 45 °, and the incident angle and the exit angle of the laser light at the first deflecting mirror 205 and the incident angle and the exit angle at the second deflecting mirror 203 are respectively substantially 45 °.

The laser is reflected to the second fixed reflector 203 through the first fixed reflector 202, and the second fixed reflector 203 reflects the laser to the light beam conversion device 204; after the light beam conversion device 204 converts the laser light, the output laser light is reflected to the second deflection mirror 206 through the first deflection mirror 205, the second deflection mirror 206 is configured to reflect the laser light to the laser output end 212, and the laser light is stably output to the first transmission optical fiber 500 under the adjustment of the first deflection mirror 205 and the second deflection mirror 206.

Optionally, the laser adapter 200 further comprises a switch device 210 disposed at the laser input end 211, and the switch device 210 comprises a switch door for opening and closing the laser input port (the laser inlet provided at the laser input end 211) and a door driving mechanism for driving the switch door to switch between an open state and a closed state. When the switch door is opened, laser can enter the laser adapter for transmission, and when the switch door is closed, the laser is blocked from entering the laser adapter. The laser output 212 is connected to a laser coupler 214 for connection to a transmission fiber.

In addition, a support leg 213 may be further disposed below the adapter housing 201 of the laser adapter 200, the support leg 213 may be set to be height-adjustable, and the height of the support leg 213 may be adjusted to enable the laser input end 211 to be adapted to the height of the laser 100, so that the laser 100 may accurately emit laser to the laser input end 211.

The laser output by the laser adapter 200 is transmitted to the laser coupling module 3 of the microscope host 300, and the specific structure of the laser coupling module 3 refers to fig. 5 and 6:

the laser coupling module 3 includes a coupler housing 33, a dispersion compensation element 34, an acousto-optic modulator 36 and a second light beam stabilizing device, wherein the dispersion compensation element 34, the acousto-optic modulator 36 and the second light beam stabilizing device are all disposed in the coupler housing 33 and are sequentially disposed along the transmission direction of the laser.

Wherein the dispersion compensating element 34 is used for compensating negative dispersion caused by the transmission fiber during the transmission of the laser light; the acousto-optic modulator 36 is used for adjusting the intensity of the laser; the second beam stabilizing device is used for adjusting the laser transmission direction to correct the deviation of the actual position of the laser beam at the laser output end 32 of the laser coupling module 3 from the ideal position, so as to stably couple the laser into the second transmission fiber 401.

The second light beam stabilizing device may specifically include a position detector 39, at least one deflecting mirror, and a mirror adjusting mechanism connected to each deflecting mirror, fig. 6 shows that the at least one deflecting mirror includes a first deflecting mirror 37 and a second deflecting mirror 38, the position detector 39 is disposed at a position close to the laser output end 32, a second beam splitter 321 is disposed on a laser transmission path close to the laser output end 32, the second beam splitter 321 reflects a part of the split light to the position detector 39, and the position detector 39 detects position information according to the split light. Based on the position information detected by the position detector 39, the controller may control the mirror adjusting mechanism to drive the deflecting mirror to deflect to adjust the laser transmission direction, and the second beam stabilizing device is substantially similar to the first beam stabilizing device in the laser adapter 200 to adjust the laser transmission direction, and will not be described herein again.

The transmission path of the laser light is described below with reference to fig. 2 as follows:

laser emitted by the laser 100 enters from the laser input end 211, is transmitted to the first fixed reflector 202, is deflected by about 90 ° by the first fixed reflector 202, is reflected to the second fixed reflector 203, is deflected by about 90 ° by the second fixed reflector 203, is transmitted to the light beam transformation device 204, is subjected to light beam transformation by the light beam transformation device 204, is transmitted to the first deflection reflector 205, is deflected by about 90 ° by the first deflection reflector 205, is reflected to the second deflection reflector 206, is reflected to the laser output end 212 by the second deflection reflector 206, and is coupled to the first transmission optical fiber 500 connected to the laser output end 212.

The optical fiber transmitted by the first transmission optical fiber 500 enters the laser coupling module 3 from the laser input end 31, is subjected to dispersion compensation by the dispersion compensation element 34, is transmitted to the reflecting mirror 35, is deflected by about 90 degrees by the reflecting mirror 35, is transmitted to the acousto-optic modulator 36, is subjected to intensity adjustment by the acousto-optic modulator 36, is transmitted to the first deflecting reflecting mirror 37, is deflected by about 90 degrees by the first deflecting reflecting mirror 37, is reflected to the second deflecting reflecting mirror 38, is reflected to the laser output end 32 by the second deflecting reflecting mirror 38, and is coupled into the second transmission optical fiber 401 connected to the laser output end 32.

In one embodiment of the present application, the microscope host 300 further includes a fluorescence collection module and a scan control module;

the scanning control module is configured to be connected to the microscope probe 400 through a control cable 403, and is configured to control the microscope probe 400 to perform laser scanning to generate a fluorescence signal;

the fluorescence collection module is connected to the microscope probe 400 through the fluorescence collection fiber 402, and is configured to collect a fluorescence signal output by the microscope probe 400 through the fluorescence collection fiber 402, where the fluorescence signal can be converted into an electrical signal and transmitted to a computer for imaging display.

In one embodiment, the microscope main body 300 further comprises a wide field search module 4, and the wide field search module 4 is configured to perform wide field imaging on the living body to search a target area for installing the microscope probe 400 on the living body. The wide field searching module 4 is a device capable of imaging a large visual field area of a living body, single photon fluorescence imaging can be adopted, imaging of the wide field searching module 4 can be transmitted to a computer to be displayed, an ocular lens can be arranged on the wide field searching module 4, and imaging can be directly observed from the ocular lens.

Fig. 7-9 show a microscope main body 300 in an embodiment, and the microscope main body 300 includes a mounting main body 1, and a wide field searching module 4, a laser coupling module 3, a fluorescence collecting module and a scanning control module integrated on the mounting main body 1.

The microscope main unit 300 in this embodiment integrates all the functional modules to form an integral structure, is compact in arrangement, can greatly reduce the occupied space, is suitable for various laboratories, and is set as a complete machine, so that outgoing lines are neat, neat and attractive. In addition, this microscope host computer is small, and is portable, easily carries the change place, can quick adjustment this microscope host computer position and direction to some experimental demands simultaneously, conveniently matches more applications. In addition, the microscope host is convenient for quick field installation and maintenance.

Optionally, the microscope main body 300 may further include a moving module 7 disposed on the mounting body 1, the moving module 7 is configured to carry a living body and can drive the living body to move in multiple directions, and the wide field searching module 4 is configured to search a visual field of the living body located on the moving module 7.

Specifically, the living body may be directly mounted on the moving module 7, and specifically, the living body may be limited by providing a clamping or limiting structure on the moving module 7. It is also possible to first mount the living body on the living body installation device 8 and then fix the living body installation device 8 on the moving module 7, and the moving module 7 can move with the living body installation device 8 to adjust the position of the living body, whereby the wide-field searching module 4 can image different areas of the living body to search for the target position of interest. The moving module 7 may be a multi-axis moving platform, and can move in multiple directions, up, down, left, right, front, and back.

As shown in fig. 9 and 10, when a living body is subjected to wide-field imaging using the wide-field search module 4, the living body is mounted on the living body mounting device 8, and then the living body mounting device 8 is fixed on the moving module 7. The living body may be a mouse or other animal.

The microscope main body 300 further includes a field search adapter 9 mounted on the mounting body 1, the field search adapter 9 including a probe mounting assembly 91 for detachably mounting the microscope probe 400, and a switching mechanism provided to be able to switch the probe mounting assembly 91 to the first position and the second position.

When the probe mounting assembly 91 is in the first position, the microscope probe 400 mounted on the probe mounting assembly 91 avoids the optical path between the wide field searching module 4 and the living body, and when the probe mounting assembly 91 is in the second position, the microscope probe 400 is aligned with the optical path of the wide field searching module 4.

The switching mechanism may be configured to switch the probe mounting assembly 91 between two positions by manually pushing and pulling, and specifically, a grip portion (not shown) may be disposed on the switching mechanism for facilitating a hand to hold, when the probe mounting assembly 91 is pushed by the grip portion, the probe mounting assembly 91 may move to the first position, and when the probe mounting assembly 91 is pulled in an opposite direction, the probe mounting assembly 91 may move to the second position.

Further, the field searching adapter 9 may be provided with an objective lens 43, when the field searching adapter 9 is mounted on the mounting body 1, the objective lens 43 is aligned with the optical path of the wide field searching module 4, and the microscope probe 400 mounted on the probe mounting assembly 91 is adjusted to be aligned with the optical path of the wide field searching module 4 when the probe mounting assembly is in the second position. When adopting wide field of vision to look for module 4 and carrying out wide field of vision formation of image, adopt switching mechanism to switch probe installation component 91 to the first position, after wide field of vision module 4 finds the target area on the live body, again with probe installation component 91 switch to the second position, then take off microscope probe 400 on the probe installation component 91, fix on the probe installed part of installation on the live body, specifically install the position (can fix through sticky mode) that corresponds with the target area that seeks at the probe installed part.

After the microscope probe 400 is mounted on the probe mount, a living body (for example, a mouse) can be detached from the living body mounting device 8, and the mouse is released to be freely movable, whereby the freely movable mouse can be observed by fluorescence imaging through the microscope probe 400.

In one embodiment, the microscope main body 300 further includes a light-shielding door 2 installed on the installation body 1 to be opened and closed. When the light-shielding door 2 is in a closed state, a closed space is formed between the installation body 1 and the light-shielding door 2, the moving module 7 is positioned in the closed space, and the wide-field searching module 4 is arranged to perform wide-field searching on living bodies on the moving module 7 positioned in the closed space.

Set up light-shielding door 2 and can guarantee that the live body is located the darkroom when wide field search module 4 images the live body, can reach higher formation of image SNR, set up light-shielding door 2 moreover and need not to set up special light-proof environment again (for example set up big aircraft bonnet, or close the light etc. in laboratory).

Alternatively, as shown in fig. 7 and 8, the light-shielding doors 2 may be provided in two, in a half-open manner, that is, two light-shielding doors 2 are respectively rotatably mounted on the mounting body 1 to be capable of being closed when rotated toward each other and being opened by being rotated away from each other. Fig. 7 shows a state where the two light-shielding doors 2 are closed, and fig. 8 shows a state where the two light-shielding doors 2 are opened. It is understood that only one of the light-shielding doors 2 may be provided, and the light-shielding doors may be configured to be opened and closed by being lifted or slid.

In one embodiment, the specific arrangement of the modules of the microscope main unit 300 can be seen in fig. 9, wherein the mounting body 1 includes a base 11 and a mounting frame 12 fixed on the base 11, and a support plate 13 is disposed at an upper position of the mounting frame 12.

The moving module 7 is movably arranged on the base 11 and positioned on one side of the mounting frame 12, the control box 5 positioned below the supporting plate 13 is fixed on the other side of the mounting frame 12, and the scanning control module and the fluorescence collection module are arranged in the control box 5; the laser coupling module 3 is mounted on the support plate 13, and the wide-field searching module 4 is mounted above the moving module 7. The light shielding door 2 is installed on a side of the mounting frame 12 facing the moving module 7, and is used for forming a closed space on a side of the mounting frame 12 having the moving module 7, and a light path of the wide field searching module 4 located above can be aligned with the closed space.

Optionally, the wide field search module 4 is installed on the laser coupling module 3, an optical path through hole 331 penetrating from top to bottom is formed in the laser coupling module 3, and the optical path of the wide field search module 4 is configured to pass through the optical path through hole 331 downward. The wide field searching module 4 may include a fluorescent light source 41 and a camera 42, and an optical path of the camera 42 passes through the optical path through hole 331 downward to reach the living body on the moving module 7, so as to realize wide-field imaging of the living body.

The laser coupling module 3 further includes a driver 361 for driving the acousto-optic modulator 36 and a cooling mechanism for cooling the driver 361, both of which are located on the upper surface of the coupler housing 33. The cooling mechanism may include a heat dissipating fin 362 for dissipating heat of the driver 361, and a fan 363 for dissipating heat of the heat dissipating fin 362.

Because the driver 361 has large driving radio frequency power, the risk of high-power radio frequency signal interference is increased when the driver 361 is placed in the coupler shell 33, and because the driver 361 has large heat productivity, the deformation of a precise optical system flat plate is easily caused, the temperature in a cavity is increased, and the performance of equipment is influenced, the driver 361 is arranged outside the coupler shell 33, and a heat radiation fin 362 and a fan 363 are added for heat radiation.

The microscope main body 300 may further include a cover 10 for covering the wide field search module 4, the driver 361 and the cooling mechanism, and the cover 10 not only protects but also contributes to the appearance.

Fig. 11 and 12 show the structure of the control box 5, a fluorescence collection module 53 and a main control circuit board 54 are arranged in the control box 5, the fluorescence collection module 53 is located above the main control circuit board 54, the main control circuit board 54 includes a scanning control module, and may further include a driving circuit board for controlling the laser coupling module 3, the visual field searching module 4, and an indicator light, a light sensor, a temperature and humidity sensor, etc.

Optionally, the fluorescence collection module 53 includes a beam splitter and at least two beam splitting and collecting modules, and the fluorescence signals collected by the fluorescence collection fiber 402 from the microscope probe 400 are split into at least two paths of fluorescence signals by the beam splitter and then collected by the at least two beam splitting and collecting modules, respectively.

The control box 5 may also be provided with a signal processing module, which is configured to process the signal output by the fluorescence collecting module 53 and transmit the processed signal to a computer for display. For example, the fluorescence signal collected by the fluorescence collection module 53 is converted into an electrical signal, amplified, collected and recombined by high-speed AD collection, and then transmitted to a computer for display.

Optionally, the control box 5 is provided with a first interface 51 for connecting the control cable 403 and a second interface 52 for connecting the fluorescence collection fiber 402, and both the first interface 51 and the second interface 52 are located at the upper position on the same side of the control box 5.

The laser coupling module 3 is located above the control box 5, and the laser output end 32 of the laser coupling module 3 for connecting the second transmission fiber 401 is located on the same side as the first interface 51 and the second interface 52 of the control box 5.

Through setting up laser coupling module 3 in the top of control box 5, the first interface 51 and the second interface 52 of control box 5 are located the top position of box, and the laser output end 32 of laser coupling module 3 is located same one side with the first interface 51 and the second interface 52 of control box 5, can be so that the second transmission fiber 401 that laser output end 32 is connected, fluorescence collection optic fibre 402 and control cable 403 are close to each other, do benefit to and walk the line rule and pleasing to the eye, and can make each cable assemble into the bus cable, for example can assemble the back and wrap up into total cable through the line skin, conveniently accomodate through storage device 6.

In addition, the outlet ends of the second transmission fiber 401, the fluorescence collection fiber 402 and the control cable 403 are arranged at the upper position of the control box 5, so that more ethological devices can be conveniently adapted, and the reduction of the length of the cable is facilitated. For example, when a mouse with the microscope probe 400 is freely placed in the living body action box, the cable may be provided to facilitate the downward insertion of the microscope probe 400 into the living body action box.

In one embodiment, the microscope main body 300 further comprises a receiving device 6, wherein the receiving device 6 is installed on the side of the control box 5 having the first interface 51 and the second interface 52, and since the second transmission fiber 401, the fluorescence collection fiber 402 and the control cable 403 are all located on the side, the receiving device 6 can conveniently receive the microscope probe 400 and the cable connected with the microscope probe 400 and comprising the second transmission fiber 401, the fluorescence collection fiber 402 and the control cable 403.

As shown in fig. 14, the storage device 6 includes: the microscope probe 400 is attached to the probe holder 62 after the cable is wound around the winding drum 611, and the storage body 61 having the winding drum 611 and the probe holder 62 are provided.

Specifically, the microscope probe 400 to which the cable is connected is inserted into the storage body 61 of the storage device 6 from the outlet of the control box 5, and then is passed out to the annular space outside the winding drum 611 and wound around the winding drum 611, and the microscope probe 400 may be mounted on the probe holder 62. Wherein, the cable is around on the winding cylinder 611 back, and when the one end that is connected with microscope probe 400 outwards stretched out to probe mounting bracket 62, the cable can block in the card wire casing 613 that sets up on accomodating main part 61, can avoid the cable loose from the winding cylinder 611, but also can avoid driving the condition that the probe breaks away from the probe support because the cable swing or loose.

Accomodate through storage device 6, cable and probe can not touched easily, pressed and lead to damaging to can avoid the cable to place the condition that leads to the winding to tie a knot at will and the condition that the cable is buckled at will and lead to inside optic fibre damage, accomodate the condition that cable and probe are neater pleasing to the eye more through storage device 6 in addition, do benefit to and promote visual effect.

In addition, the storage device 6 may further include an indicator lamp disposed in the storage body 1 (hidden from view by the light blocking plate 612 in the drawing), and a light-transmitting cover may be disposed on the light blocking plate 612 of the storage body 61, so that light emitted from the indicator lamp can be seen from the light-transmitting cover.

The indicator light may be set to indicate the operating state of the optical imaging system, for example, when the controller receives a message that the laser 100 emits laser light, or when laser transmission inside the laser adapter 200 or the laser coupling module 3 is detected, the indicator light may be controlled to emit green light, for example, to indicate that the system is in the operating state, and if an abnormality of laser light in the system is detected or other abnormal states occur, the indicator light may emit red light, for example, to warn, and when the system is in the non-operating state, the ring indicator light may display yellow for internal illumination.

The storage device 6 can also be provided with a protective cover 63, the protective cover 63 is rotatably arranged on the storage main body 1 through a rotating shaft, and the protective cover 63 can also be provided with a transparent observation window 631 so as to conveniently observe the inside condition of the storage device and the equipment state indicated by the annular indicating lamp.

It is to be understood that the microscope main body 300 is not limited to the above-described configuration, and for example, the microscope main body may include only the laser coupling module 3, the fluorescence collection module, and the scan control module, that is, only the laser coupling module 3 and the control box 5 having the fluorescence collection module and the scan control module. The moving module 7, the wide-field search module 4 and the visual field search adapter 9 are assembled to form another independent apparatus for mounting the microscope probe 400 on a living body.

The application provides an optical imaging system can also include the workstation, the workstation includes workstation host computer and display, and the workstation host computer is connected with microscope host computer 300, and microscope host computer 300 handles the back to the fluorescence signal of collection and transmits to the workstation host computer, and the display shows the formation of image, the workstation host computer still sends control command to microscope host computer 300, then each control circuit in the control box 5 carries out the control of each spare part.

The optical imaging system may further include a behavioural test device that provides a living body in which the microscope probe 400 is installed with a moving space. For example, a mouse with the microscope probe 400 attached thereto is placed in an ethological test apparatus and allowed to move freely, and the state of neurons in the freely moving state of the mouse can be detected.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modifications, equivalents and the like that are within the spirit and principle of the present application should be included in the scope of the present application.

Claims (10)

1. An optical imaging system, characterized in that the optical imaging system comprises: the device comprises a laser, a laser adapter and a microscope host; wherein, the first and the second end of the pipe are connected with each other,

the laser is used for emitting laser to the laser adapter;

the laser adapter is used for receiving the laser emitted by the laser, adjusting and adapting the laser, and then transmitting the adjusted and adapted laser to the microscope host;

the microscope host is arranged to transmit laser to the microscope probe and control the microscope probe to perform laser scanning on a living body to generate a fluorescence signal for imaging.

2. The optical imaging system of claim 1, further comprising a first transmission fiber coupled between the laser adapter and the microscope host, the laser adapter transmitting the adapted laser light to the microscope host through the first transmission fiber.

3. The optical imaging system of claim 2, wherein the microscope host comprises a laser coupling module, a laser input end of the laser coupling module is connected with the first transmission optical fiber, and a laser output end of the laser coupling module is connected with the microscope probe through a second transmission optical fiber;

the laser coupling module is used for adjusting the laser received from the first transmission optical fiber and then transmitting the laser to the microscope probe through the second transmission optical fiber.

4. The optical imaging system of claim 3, wherein the laser adapter comprises a first power detection device for detecting laser power;

the laser coupling module comprises a second power detection device for detecting laser power.

5. The optical imaging system of claim 4, wherein the first power detection device is proximate to a laser output end of the laser adapter and the second power detection device is proximate to a laser input end of the laser coupling module.

6. The optical imaging system of any of claims 1-5, wherein the laser adapter comprises an adapter housing and a beam transforming device and a first beam stabilizing device located within the adapter housing; wherein the content of the first and second substances,

the light beam conversion device is used for carrying out light beam conversion on the laser entering the laser adapter;

the first light beam stabilizing device is arranged at the downstream of the light beam changing device along the laser transmission direction and is used for adjusting the transmission direction of the laser so as to correct the deviation of the actual position and the ideal position of the laser beam at the laser output end of the laser adapter.

7. The optical imaging system of any one of claims 3 to 5, wherein the laser coupling module comprises a coupler housing, a dispersion compensation element, an acousto-optic modulator and a second beam stabilizing device, wherein the dispersion compensation element, the acousto-optic modulator and the second beam stabilizing device are all arranged in the coupler housing and are arranged in sequence along the transmission direction of the laser; wherein the content of the first and second substances,

the dispersion compensation element is used for compensating negative dispersion caused by the transmission optical fiber in the process of transmitting laser light;

the acousto-optic modulator is used for adjusting the intensity of laser;

the second light beam stabilizing device is used for adjusting the laser transmission direction so as to correct the deviation between the actual position and the ideal position of the laser beam at the laser output end of the laser coupling module.

8. The optical imaging system of any one of claims 3 to 5, wherein the microscope host further comprises a fluorescence acquisition module and a scan control module;

the scanning control module is connected with the microscope probe through a control cable and is used for controlling the microscope probe to carry out laser scanning so as to generate a fluorescence signal;

the fluorescence collection module is connected with the microscope probe through a fluorescence collection optical fiber and used for collecting the fluorescence signal output by the microscope probe through the fluorescence collection optical fiber.

9. The optical imaging system of claim 8, wherein the microscope host further comprises a wide field search module;

the wide field searching module is configured to perform wide field imaging on a living body to search a target area for mounting the microscope probe on the living body.

10. The optical imaging system of claim 8, further comprising a table, the table comprising a table host and a display;

the workbench host is connected with the microscope host, the microscope host processes the collected fluorescence signals and then transmits the processed fluorescence signals to the workbench host, and the display displays images;

the workbench host also sends a control instruction to the microscope host.

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