CN115629166B - Sample pretreatment device and pretreatment method for food detection - Google Patents
Sample pretreatment device and pretreatment method for food detection Download PDFInfo
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- CN115629166B CN115629166B CN202211265747.0A CN202211265747A CN115629166B CN 115629166 B CN115629166 B CN 115629166B CN 202211265747 A CN202211265747 A CN 202211265747A CN 115629166 B CN115629166 B CN 115629166B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention relates to the technical field of detection sample treatment, and discloses a sample pretreatment device for food detection, which comprises a homogenizer body, wherein the top of the homogenizer body is hinged with a cover plate through a hinge, the surface of the cover plate is fixedly connected with a convex plate, the surface of the cover plate is provided with two grooves, the front surface of the homogenizer body is provided with a control panel, and a treatment groove is arranged in the homogenizer body.
Description
Technical Field
The invention relates to the technical field of detection sample treatment, in particular to a sample pretreatment device and a pretreatment method for food detection.
Background
Rosin is an important chemical raw material commonly used in the field of paper making or production of plant inhibitors, has good adhesion after being heated, and is widely used in the secondary unhairing process of livestock and poultry slaughtering processing. Rosin acid and dehydrorosin acid are main components of rosin, belong to mushroom compounds, are slightly toxic, and can have adverse effects on human health if being taken for a long time. Researches show that the abietic acid, the dehydroabietic acid and oxidation products thereof are skin contact allergens, have adverse effects on the growth and development of fishes, can reduce the reproductive capacity of daphnia squarrosomas, and cause the remarkable reduction of the biological activities of human epithelial cells and fibroblasts, and the necrosis of human erythrocytes and multinuclear leukocytes.
The current chinese patent is CN 214844372U's a sample pretreatment device for food detection, including the base, the welding of base upper end has a first pinion rack that transversely sets up, is located the vertical rigid coupling of still symmetry has two parallel arrangement's track, two on the base at both ends around the first pinion rack the common sliding connection has the connecting block of level setting in the middle of the track.
The device and the method are only characterized in that the connecting block which can circularly move back and forth after being driven by the motor drives the shaking bottles to synchronously move, reagents in the shaking bottles are uniformly mixed, but the crushing effect of epidermal tissues in the bottles is poor only by simple shaking, and the crushing is not thorough, so that the device and the method for pretreating samples for food detection are provided.
Disclosure of Invention
Technical problem to be solved
In order to overcome the defects in the prior art, the invention provides a sample pretreatment device and a pretreatment method for food detection, which aim to solve the problems in the background art.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a sample pretreatment device for food detection comprises a homogenizer body, wherein the top of the homogenizer body is hinged with a cover plate through a hinge, the inner side of the cover plate is fixedly connected with a convex plate, two grooves are formed in the inner side of the cover plate, a control panel is arranged on the front side of the homogenizer body, and a treatment tank is arranged in the homogenizer body;
the tank wall of the treatment tank is fixedly connected with a cross rod, the upper end of the cross rod is provided with an ultrasonic treatment device, and the lower end of the cross rod is provided with a vortex device.
Preferably, the two side groove walls of the processing groove are provided with movable grooves, the two side groove walls of the processing groove are also provided with communicating grooves, the communicating grooves are communicated with the movable grooves, the top of the homogenizer body is provided with two slots, and the two slots are respectively communicated with the two movable grooves.
Preferably, the ultrasonic treatment device comprises a collection chamber, the front surface and the back surface of the collection chamber are fixedly connected with connecting plates, the connecting plates extend to the inner part of the movable groove to be contacted with the wall of the movable groove, the bottom of the inner wall of the collection chamber is provided with an ultrasonic extractor, and the inner wall of the collection chamber is fixedly connected with a protective cover.
Preferably, the bottom of the collecting chamber is arranged in a downward arc shape, the bottom of the collecting chamber is connected with a communicating pipe, the communicating pipe is wound on a cross rod through a rope, two inserting rods are inserted into the connecting plates and penetrate through the two slots respectively, the diameter of each inserting rod is consistent with the aperture of each slot, and the two inserting rods are slightly deeper than the homogenizer body.
Preferably, the vortex device includes power component, power component output end fixedly connected with backup pad, the equal fixedly connected with baffle of backup pad left and right sides, two the baffle top all rotates through the pivot and is connected with the deflector, two the indent has all been seted up on the deflector surface, the backup pad surface is provided with two and places the piece, every place the piece inside five standing grooves of all having seted up, every the centrifuging tube has all been inserted to the standing groove inside, backup pad top axis department fixedly connected with stop gear.
Preferably, stop gear includes the montant, two fixed plates of montant surface fixed connection, the inside draw-in groove of having seted up of montant, and draw-in groove cell wall fixed connection has the elastic component, the montant surface is provided with spacing, spacing and backup pad top fixed connection, the one end that the backup pad was kept away from to spacing is located inside the draw-in groove and with the contact of draw-in groove cell wall, montant bottom fixedly connected with spring, spring bottom and backup pad top fixed connection, the extrusion frame has been taken to spacing surface, extrusion frame bottom fixedly connected with support frame, support frame bottom and backup pad top contact, support frame top and spacing surface contact, spacing surface fixed connection has the limiting plate, and limiting plate and montant surface contact.
Preferably, the elastic part comprises an elastic frame, the elastic frame is fixedly connected with the groove wall of the clamping groove, the upper end and the lower end of the elastic frame are both provided with a notch, and the inner wall of the elastic frame is fixedly connected with an elastic plate.
A pretreatment method of a sample for detection comprises the following steps:
s1, preparing standard substance information and a standard solution, accurately sucking mixed standard intermediate liquid with different volumes respectively, and diluting the mixed standard intermediate liquid into a series of standard working liquids by using acetonitrile, wherein the concentrations of dehydroabietic acid and abietic acid in the standard series are 0.5 mu g/mL, 1.0 mu g/mL, 2.0 mu g/mL, 5.0 mu g/mL and 10.0 mu g/mL;
s2, selecting the type of a sample matrix;
s3, optimizing the pretreatment of the sample, selecting acetonitrile as an extracting agent, and extracting the investigation substrate by three extraction modes of ultrasound, vortex and homogenate;
s4, optimizing instrument conditions, and determining final chromatographic conditions by optimizing detection wavelength and optimizing mobile phase conditions;
and S5, verifying methodology by using the linear range, the linear equation, the sensitivity, the accuracy and the repeatability of the method and the verification condition of the method.
Compared with the prior art, the invention provides a sample pretreatment device and a pretreatment method for food detection, which have the following beneficial effects:
1. according to the invention, the ultrasonic treatment device is combined with the vortex device, so that the extraction efficiency of the matrix is higher, the residual matrix in the extracting solution is effectively crushed more thoroughly, and the homogenization effect is achieved.
2. According to the invention, the insertion rods are inserted into the surface of the connecting plate and penetrate through the insertion grooves, so that the connecting plate and the collection chamber can be limited through the insertion grooves, the phenomenon that the collection chamber slides randomly in the movable groove due to vibration generated when the power assembly works is avoided, and meanwhile, the solution in the collection chamber can be blocked while the collection chamber is driven to vibrate due to the arrangement of the protective cover on the inner wall of the collection chamber, so that the phenomenon that the solution in the collection chamber splashes out of the collection chamber along with vibration is avoided.
3. According to the centrifugal tube, the vertical rod is pulled upwards and slightly twisted, so that the vertical rod moves upwards through the spring, the deflection plate is shifted, the top of the centrifugal tube is blocked after the deflection plate deflects through the partition plate, the vertical plate is reset through the spring, and the fixing plate is driven to be clamped into the pressing groove formed in the side edge of the deflection plate, so that the deflection plate is limited, and the possibility that a solution in the centrifugal tube is thrown out is avoided.
4. According to the invention, the clamping groove is formed in the vertical rod, the tail end of the limiting frame is clamped into the clamping groove, and then the limiting plate is contacted with the surface of the vertical rod, so that the vertical rod can be limited by the limiting frame and the limiting plate, the situation that the centrifugal tube is not tightly blocked by the deflection plate due to the fact that the vertical rod shakes along with the supporting plate is avoided, meanwhile, the extrusion frame is clamped into the middle end of the limiting frame, the limiting frame and the extrusion frame are supported by the supporting frame, the limiting effect of the limiting frame on the vertical rod is better ensured, meanwhile, the extrusion frame can also press the deflection plate through two ends, and the blocking effect of the deflection plate on the centrifugal tube is better ensured.
5. According to the invention, the elastic frame is arranged in the clamping groove, and after the elastic frame and the clamping groove are fixed, two ends of the elastic frame are opened through the elastic plate to separate the limiting frame, so that the situation that the limiting frame is separated from the vertical rod from the clamping groove can be avoided.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a cross-sectional view of the homogenizer body of the present invention;
FIG. 4 is an enlarged view of A in FIG. 3 according to the present invention;
FIG. 5 is a schematic view of the ultrasonic treatment apparatus of the present invention;
FIG. 6 is a schematic structural view of the swirling device of the present invention;
FIG. 7 is a perspective view of the support plate and stop mechanism of the present invention;
fig. 8 is an exploded view of the spacing mechanism of the present invention.
In the figure: homogenizer organism 1, apron 2, flange 3, recess 4, control panel 5, treatment trough 6, horizontal pole 7, ultrasonic treatment device 8, collection room 81, connecting plate 82, inserted bar 83, protection casing 84, ultrasonic extractor 85, communicating pipe 86, vortex device 9, power component 91, backup pad 92, baffle 93, deflector plate 94, indent 95, centrifuging tube 96, place the piece 97, standing groove 98, stop gear 99, montant 991, draw-in groove 992, spacing frame 993, spring 994, fixed plate 995, support frame 996, extrusion frame 997, limiting plate 998, elastic member 999, elastic frame 9991, elastic plate 9992, fluting 9993, activity groove 10, communicating groove 11, slot 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to explain the present invention and should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1-8, the present invention provides a technical solution: a sample pretreatment device for food detection comprises a homogenizer body 1, wherein the top of the homogenizer body 1 is connected with a cover plate 2 through a hinge, the inner side of the cover plate 2 is fixedly connected with a convex plate 3, the inner side of the cover plate 2 is provided with two grooves 4, the front surface of the homogenizer body 1 is provided with a control panel 5, a treatment tank 6 is arranged inside the homogenizer body 1, the tank wall of the treatment tank 6 is fixedly connected with a cross rod 7, the upper end of the cross rod 7 is provided with an ultrasonic treatment device 8, the lower end of the cross rod 7 is provided with a vortex device 9, the tank walls at two sides of the treatment tank 6 are both provided with movable tanks 10, the tank walls at two sides of the treatment tank 6 are also provided with communication tanks 11, the communication tanks 11 are communicated with the movable tanks 10, the top of the homogenizer body 1 is provided with two slots 12, the two slots 12 are respectively communicated with the two movable tanks 10, the ultrasonic treatment device 8 is combined with the vortex device 9, the extraction efficiency of a substrate is ensured to be higher, the residual substrate in an extracting solution is effectively and thoroughly crushed, and the homogenizing effect is achieved;
the ultrasonic treatment device 8 comprises a collection chamber 81, connecting plates 82 are fixedly connected to the front side and the back side of the collection chamber 81, the connecting plates 82 extend into the movable groove 10 and are in contact with the wall of the movable groove 10, an ultrasonic extractor 85 is arranged at the bottom of the inner wall of the collection chamber 81, when the ultrasonic treatment device is used, one of beef, pork, mutton, duck meat and chicken meat is selected as an investigation substrate, after animal tissues are added into the collection chamber 81, acetonitrile is selected as an extracting agent to extract the investigation substrate, after the ultrasonic extractor 85 is started, the solvent quickly enters the investigation substrate, organic components contained in the investigation substrate are dissolved in the solvent to obtain a multi-component mixed extracting solution, and the pretreatment of the investigation substrate is completed;
collect room 81 inner wall fixedly connected with protection casing 84, collect room 81 bottom and set up for decurrent arc, and collect room 81 bottom and be connected with communicating pipe 86, communicating pipe 86 winds on horizontal pole 7 through the rope, inserted bar 83 has all been pegged graft to two connecting plates 82 inside, two inserted bars 83 run through two slots 12 respectively, the diameter of two inserted bars 83 is unanimous with the aperture of two slots 12, and two inserted bars 83 are slightly darker than homogenizer machine body 1, inserted bar 83 through pegging graft on connecting plate 82 surface, and inserted bar 83 also runs through slot 12 department simultaneously, can restrict connecting plate 82 and collection room 81 through slot 12, the vibrations that avoid collecting room 81 to produce when power component 91 works lead to taking place at will at the inside gliding condition in movable groove 10, set up protection casing 84 at the inner wall of collection room 81 simultaneously, can be driven the vibrations to its inside solution separation when collection room 81, avoid the inside solution of collection room 81 to take place along with the condition that vibrations spill from collecting room 81.
The vortex device 9 comprises a power assembly 91, an output end of the power assembly 91 is fixedly connected with a support plate 92, the left side and the right side of the support plate 92 are both fixedly connected with partition plates 93, the tops of the two partition plates 93 are both rotatably connected with deflection plates 94 through rotating shafts, the surfaces of the two deflection plates 94 are both provided with pressure grooves 95, the surface of the support plate 92 is provided with two placing blocks 97, the placing blocks 97 are made of foamed plastics, five placing grooves 98 are formed in each placing block 97, a centrifugal tube 96 is inserted in each placing groove 98, the obtained mixed extracting solution can flow out along a communicating tube 86 and drip in the centrifugal tube 96, glass steel balls are added in the centrifugal tube 96, the inserted centrifugal tube 96 in each placing groove 98 can be driven by starting the power assembly 91 to carry out vortex treatment, when the extracting solution is subjected to vortex mixing, high-speed motion through collision between the steel balls is carried out, then the steel balls between cavities are collided at high speed, residual matrixes in the extracting solution are crushed more thoroughly, and the homogenizing effect is achieved;
supporting plate 92 top axis department fixedly connected with stop gear 99, through upwards pulling montant 991 and slight wrench movement, make montant 991 shift up through spring 994, the deflector 94 is stirred to the rethread, make deflector 94 deflect the back through baffle 93 and block up centrifuge tube 96's top, make riser 991 pass through the reseing of spring 994 again, drive fixed plate 995 card go into in the indent 95 that deflector 94 side was seted up, thereby the realization is spacing to deflector 94, avoid centrifuge tube 96 in the solution by the possibility of throwing away.
The limiting mechanism 99 comprises a vertical rod 991, two fixing plates 995 are fixedly connected to the outer surface of the vertical rod 991, a clamping groove 992 is formed in the vertical rod 991, an elastic member 999 is fixedly connected to the groove wall of the clamping groove 992, a limiting frame 993 is arranged on the outer surface of the vertical rod 991, the limiting frame 993 is fixedly connected with the top of the supporting plate 92, one end of the limiting frame 993, which is far away from the supporting plate 92, is positioned in the clamping groove 992 and is in contact with the groove wall of the clamping groove 992, a spring 994 is fixedly connected to the bottom of the vertical rod 991, the bottom of the spring 994 is fixedly connected with the top of the supporting plate 92, an extrusion frame 997 is arranged on the outer surface of the limiting frame 993, a supporting frame 996 is fixedly connected to the bottom of the extrusion frame 997, the bottom of the supporting frame 996 is in contact with the top of the supporting plate 92, the top of the supporting frame 996 is in contact with the surface of the limiting frame 993, a limiting plate 998 is fixedly connected to the outer surface of the limiting frame 993, and limiting plate 998 and montant 991 outer surface contact, through set up draw-in groove 992 in montant 991 is inside, go into the inside back of draw-in groove 992 with spacing 993 end card, make limiting plate 998 and montant 991's surface contact, thereby can restrict montant 991 through spacing 993 and limiting plate 998, avoid montant 991 to rock along with backup pad 92 and lead to the deflector plate 94 to stop up the not tight condition emergence of centrifuging tube 96, simultaneously go into extrusion bracket 997 at spacing 993 middle-end card, support spacing 993 and extrusion bracket 997 through support frame 996, guarantee that spacing 993 is better to the restriction effect of montant 991, extrusion bracket 997 also can press deflector plate 94 through both ends simultaneously, guarantee that deflection plate 94 is better to the jam effect of centrifuging tube 96.
And the homogenizer that used before is when using every secondary washing tool bit, and is more troublesome, through adding glass steel ball to centrifuge tube 96 mixing crushing matrix back, because the steel ball is disposable, can directly abandon the steel ball after having smashed, does not have the drawback of washing tool bit, and is very convenient.
A pretreatment method of a sample for detection comprises the following steps:
s1, preparing standard substance information and a standard solution, accurately sucking mixed standard intermediate liquid with different volumes respectively, and diluting the mixed standard intermediate liquid into a series of standard working liquids by using acetonitrile, wherein the concentrations of dehydroabietic acid and abietic acid in the standard series are 0.5 mu g/mL, 1.0 mu g/mL, 2.0 mu g/mL, 5.0 mu g/mL and 10.0 mu g/mL;
s2, selecting the type of a sample matrix;
s3, optimizing the pretreatment of the sample, selecting acetonitrile as an extracting agent, and extracting the investigation substrate by three extraction modes of ultrasound, vortex and homogenate;
s4, optimizing instrument conditions, and determining final chromatographic conditions by optimizing detection wavelength and optimizing mobile phase conditions;
and S5, verifying methodology by using the linear range, the linear equation, the sensitivity, the accuracy and the repeatability of the method and the verification condition of the method.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a food detects uses sample pretreatment device, includes isotropic symmetry organism (1), its characterized in that: the top of the homogenizer body (1) is hinged with a cover plate (2) through a hinge, a convex plate (3) is fixedly connected to the inner side of the cover plate (2), two grooves (4) are formed in the inner side of the cover plate (2), a control panel (5) is arranged on the front side of the homogenizer body (1), and a treatment tank (6) is arranged in the homogenizer body (1);
the wall of the treatment tank (6) is fixedly connected with a cross rod (7), the upper end of the cross rod (7) is provided with an ultrasonic treatment device (8), and the lower end of the cross rod (7) is provided with a vortex device (9);
the vortex device (9) comprises a power assembly (91), a support plate (92) is fixedly connected with the output end of the power assembly (91), partition plates (93) are fixedly connected to the left side and the right side of the support plate (92), the tops of the two partition plates (93) are rotatably connected with deflection plates (94) through rotating shafts, pressure grooves (95) are formed in the surfaces of the two deflection plates (94), two placing blocks (97) are arranged on the surface of the support plate (92), five placing grooves (98) are formed in each placing block (97), a centrifugal tube (96) is inserted in each placing groove (98), and a limiting mechanism (99) is fixedly connected to the middle shaft at the top of the support plate (92);
stop gear (99) are including montant (991), montant (991) outer fixed surface is connected with two fixed plates (995), montant (991) inside draw-in groove (992) of having seted up, and draw-in groove (992) cell wall fixed connection have elastic component (999), montant (991) surface is provided with spacing (993), spacing (993) and backup pad (92) top fixed connection, spacing (993) keep away from backup pad (92) one end be located draw-in groove (992) inside and with draw-in groove (992) cell wall contact, montant (991) bottom fixedly connected with spring (994), spring (994) bottom and backup pad (92) top fixed connection, spacing (993) surface has been taken extrusion frame (997), extrusion frame (997) bottom fixedly connected with support frame (996), support frame (996) bottom and backup pad (92) top contact, support frame (996) top and spacing (993) surface contact, spacing (993) outer fixed surface is connected with limiting plate (998), and limiting plate (998) and 991) outer surface contact.
2. The sample pretreatment device for food detection according to claim 1, characterized in that: the processing tank is characterized in that movable grooves (10) are formed in the two side tank walls of the processing tank (6), communicating grooves (11) are further formed in the two side tank walls of the processing tank (6), the communicating grooves (11) are communicated with the movable grooves (10), two inserting grooves (12) are formed in the top of the homogenizer body (1), and the two inserting grooves (12) are respectively communicated with the two movable grooves (10).
3. The sample pretreatment device for food detection according to claim 2, characterized in that: ultrasonic treatment device (8) are including collecting room (81), collect equal fixedly connected with connecting plate (82) in room (81) tow sides, connecting plate (82) extend to the cell wall of activity groove (10) inside and activity groove (10) and contact, it is provided with supersound extractor (85) to collect room (81) inner wall bottom, it has protection casing (84) to collect room (81) inner wall fixedly connected with.
4. The sample pretreatment device for food detection according to claim 3, characterized in that: the bottom of the collecting chamber (81) is arranged in a downward arc shape, the bottom of the collecting chamber (81) is connected with a communicating pipe (86), the communicating pipe (86) is wound on the cross rod (7) through a rope, two inserting rods (83) are inserted into the connecting plates (82), the inserting rods (83) penetrate through the slots (12), the diameter of each inserting rod (83) is consistent with the aperture of each slot (12), and the two inserting rods (83) are slightly deeper than the homogenizer body (1).
5. The sample pretreatment apparatus for food detection according to claim 1, characterized in that: elastic component (999) are including elastic frame (9991), elastic frame (9991) and draw-in groove (992) cell wall fixed connection, fluting (9993) have all been seted up at both ends about elastic frame (9991), elastic frame (9991) inner wall fixedly connected with elastic plate (9992).
6. A method for pretreating a sample for testing to which the sample pretreatment device for food testing according to claim 1 is applied, comprising the steps of:
s1, preparing standard substance information and a standard solution, accurately absorbing mixed standard intermediate solutions with different volumes respectively, and diluting the mixed standard intermediate solutions into a series of standard working solutions by using acetonitrile, wherein the concentrations of dehydroabietic acid and abietic acid in the standard series are 0.5 mu g/mL, 1.0 mu g/mL, 2.0 mu g/mL, 5.0 mu g/mL and 10.0 mu g/mL;
s2, selecting the type of a sample matrix;
s3, optimizing the pretreatment of the sample, selecting acetonitrile as an extracting agent, and extracting the investigation substrate by three extraction modes of ultrasound, vortex and homogenate;
s4, optimizing instrument conditions, and determining final chromatographic conditions by optimizing detection wavelength and optimizing mobile phase conditions;
and S5, verifying methodology by using the linear range, the linear equation, the sensitivity, the accuracy and the repeatability of the method and the verification condition of the method.
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