CN115624109A

CN115624109A - Broiler silage and preparation method and application thereof

Info

Publication number: CN115624109A
Application number: CN202211184469.6A
Authority: CN
Inventors: 黄志朝; 易显菊; 滕晓婳; 易显凤; 吴思谦; 庞天德; 邓素媛
Original assignee: Guangxi Agricultural Vocational University
Current assignee: Guangxi Agricultural Vocational University
Priority date: 2022-09-27
Filing date: 2022-09-27
Publication date: 2023-01-20

Abstract

The invention relates to the field of silage processing, in particular to broiler silage and a preparation method and application thereof, the invention researches the influence of 3 feeds such as elephant grass (including Guijian elephant grass and purple elephant grass), alfalfa, kudzu and the like on the growth of Jinling partridge, and a large number of animal experiments prove that: the fermented feed with a single fiber source has no obvious promotion effect on the growth of the Jinling spotted chickens, and after reasonable formula optimization, the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu vine silage have a good promotion effect on the growth of the Jinling spotted chickens, and simultaneously can effectively reduce the feed weight ratio of the silage and improve the utilization rate of the silage.

Description

Broiler silage as well as preparation method and application thereof

[ technical field ] A method for producing a semiconductor device

The invention relates to the technical field of silage processing, in particular to broiler silage and a preparation method and application thereof.

[ background ] A method for producing a semiconductor device

Dietary fiber is composed of cellulose, hemicellulose and lignin, structural compounds and non-structural compounds. In recent years, researches show that a large number of enzymes with biological activity and a plurality of functional substances are arranged on plant cell walls, and the components can effectively improve the absorption and utilization of nutrient substances by animal bodies, thereby promoting the healthy growth of animals. Fiber is an essential ingredient in poultry feed. On the one hand, poultry do not have the corresponding digestive enzymes and have a poor ability to digest fiber, and thus whether fiber is added or not is considered an anti-nutritional ingredient, and on the other hand, many types of fiber are considered to improve the quality of poultry manure, improve intestinal health, eliminate hunger, promote health and growth of poultry. In these studies it was found that different kinds of cellulases have a great effect on the decomposition of cellulose. They can also promote the development of organs of the broiler chickens, promote the secretion of related enzymes, promote the digestion of nutrient substances and improve the performance of the broiler chickens. Thus, fiber is considered a functional feed additive. Previous studies have investigated the effect of dietary fiber on animal growth and development, and found in the studies: different fiber source diets have different effects on the growth and development of animals. In the prior art, under the same feeding conditions, the FREIRE test shows that: the addition of a proper amount of alfalfa fiber can promote the growth of piglets; and the addition of a large amount of bean cakes can result in the reduction of the feed conversion rate. Therefore, it is recommended to select a suitable fiber source as a ration additive. FREIRE and others found that wheat bran fiber was effective in ensuring daily nitrogen retention in pigs when the pigs were fed daily a diet containing alfalfa fiber, soybean fiber, beet pulp and wheat bran fiber. The results of the juxiachun and the like for feeding white goose in Yangzhou by using the alfalfa and the rice hull grass powder as fiber sources show that: the two fiber sources have no influence on the growth performance of the Yangzhou white goose at 1-4 weeks. Therefore, different dietary fibers have different effects on different animals, and deep research and screening are required to obtain the dietary fiber feed suitable for different animals.

Because of the influence of the policy of banning grazing, how to realize the higher growth of economy becomes the difficult problem that awaits solution urgently, the above-mentioned problem has effectively been solved in the appearance of fermented silage, and in addition, adopt the fermentation process dietary fiber feed not only can improve the productivity of beasts and birds, but also can reduce the disease and take place, improve the internal condition of animal, promote beasts and birds meat egg class food product quality, reduce the discarded object and discharge, protect the environment and prevent pollution, be one of the most effective methods of solving antibiotic residue, promoting animal growth and reduction in production cost at present.

Earlier researches show that the fermented feed can improve the weight of the Jinling spotted chickens and improve the production performance and slaughtering performance of the Jinling spotted chickens, but whether certain influence is exerted on blood physiological indexes of the Jinling spotted chickens is not clear. In northern areas of China, due to the lack of pasture resources such as high-quality alfalfa and the like, the healthy and rapid development of poultry industry in the areas is limited. The fermented feed has the advantages of comprehensive nutrition, good palatability and the like, and is an ideal green and environment-friendly feed raw material. Therefore, the influence of the fermented feed on the production performance and slaughtering performance and the influence of the fermented forage grass on the physiological indexes of blood are researched, and a theoretical basis is provided for popularization and application in the feeding of the fermented forage grass, the food and the livestock.

[ summary of the invention ]

In view of the above, there is a need for a fermented feed for the production performance and slaughter performance of Jinling chicken, which is produced from a dietary fiber material and has a good effect of improving the meat quality of Jinling chicken.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the broiler silage comprises the following raw materials in percentage by weight: 57.52 percent of corn, 22.05 percent of soybean meal, 8.73 percent of wheat bran, 2.3 percent of fish meal, 1.4 percent of stone powder and 8 percent of fermented forage grass; the fermented forage grass consists of fermented purple elephant grass, fermented alfalfa and fermented kudzu.

Furthermore, the mass ratio of the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu in the fermented forage grass is 1. Furthermore, the invention also comprises the application of the broiler silage in improving the weight of the Jinling spotted chicken and/or reducing the abdominal fat rate, wherein the mass ratio of the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu in the fermented forage grass is 1.

The invention also comprises a method for preparing the broiler silage, which comprises the following steps:

(1) preparing fermented forage grass: respectively inoculating purple elephant grass, alfalfa and kudzu vine into silage agents for fermentation to obtain fermented purple elephant grass, fermented alfalfa and fermented kudzu vine, and then mixing the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu vine according to a mass ratio of 1-2-6; the fermentation time of the purple elephant grass, the alfalfa and the kudzu is 25-30d;

(2) weighing the raw materials according to the weight percentage, fully mixing, stacking with fermented forage grass for 20-25d, taking out the stack retting product, air drying, crushing and granulating to obtain the silage.

The invention also comprises a method for feeding Jinling Huaji by using the broiler silage, which is characterized in that the method comprises the steps of feeding the just-born Jinling Huaji with a basic feed without adding fermented forage grass, and feeding the Jinling Huaji with the broiler silage added with the fermented forage grass instead of the basic feed after 1 week, wherein the silage is prepared by mixing the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu according to the mass ratio of 1-2-6.

The invention has the following beneficial effects:

1. the invention researches the influence of 3 forage grasses such as elephant grass (including Guijian leading elephant grass and purple elephant grass), alfalfa, kudzu and the like on the growth of Jinling partridge chickens, and a large number of animal experiments prove that: the fermented forage grass with a single fiber source is adopted, and has no obvious promotion effect on the growth of the Jinling spotted chickens due to single nutritional ingredients, and after reasonable formula optimization, the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu silage play a good promotion effect on the growth of the Jinling spotted chickens, and meanwhile, the feed weight ratio of the silage can be effectively reduced, and the utilization rate of the silage is improved, and continuous researches show that when the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu silage are mixed according to the mass ratio of (2-6).

[ description of the drawings ]

FIG. 1 is a graph showing the effect of fermented feed from different fiber sources on the performance of chickens from Jinling;

FIG. 2 is a graph of the effect of fermented feed from different fiber sources on slaughter performance of Jinling spotted chickens;

FIG. 3 is a graph showing the effect of fermented feeds from different fiber sources on the physiological indicators of the blood of the Jinling spotted chickens.

[ detailed description ] embodiments

All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.

Any feature disclosed in this specification (including any accompanying claims, abstract) is an example of a generic series of equivalent or similar features, unless expressly stated otherwise.

Example 1:

the present example mainly studies the influence of different fermented feeds on the growth of the jinling spotted maigre chickens, as follows:

1. test materials:

the Guangxi Jinling Huaji test field is provided by Guangxi Jinling farming and animal husbandry limited company, guangxi Dafuhua farming and animal husbandry limited company provides basic compound feed, and Guangxi Zhuang nationality autonomous region animal husbandry institute provides fermented alfalfa. The fermented forage grass types are respectively fermented purple elephant grass, fermented Guijian Zhenxiang elephant grass, fermented alfalfa and fermented kudzu, and are respectively marked as GG, GR, LM and LA. The composition and nutritional level of the air-dried basal diet and fermented feed are shown in table 1:

TABLE 1 air-dried basal diet composition and nutritional levels

In the embodiment, 1-day-old Jinling spotted chickens are selected, the feeding mode is cage culture, the culture environment is well ventilated, the natural illumination is realized, the humidity of about 65 percent is controlled and maintained, the chickens are fed freely and sufficiently, and the feeding times are 2 times/day.

2. Experiment design:

a random block experiment design is adopted, 1 day old Jinling flower chickens are selected, and 240 feathers of healthy Jinling flower chickens without obvious weight difference are selected and randomly divided into 5 experiment groups. One group of the four groups of fermented forage grass is used as a control group (fed by basic compound feed and recorded as CK), and the other four groups of fermented forage grass are respectively added from the first week and replace the feeding treatment of basic forage grains (the types of the added fermented forage grass are respectively fermented purple elephant grass, fermented Guiminyue elephant grass, fermented alfalfa and fermented kudzu and recorded as GG, GR, LM and LA). 48-feathered chickens were treated in each treatment group, with each 4-feathered chicken being housed in the same cage.

3. Test animals and test design

A random block experimental design is adopted, 1 day old Jinling spotted chickens are selected, and 240 feathers of healthy Jinling spotted chickens without obvious weight difference are selected and randomly divided into 5 experimental groups. One group of the four groups of fermented forage grass is used as a control group (fed by basic compound feed and recorded as CK), and the other four groups of fermented forage grass are respectively added from the first week and replace the feeding treatment of basic forage grains (the types of the added fermented forage grass are respectively fermented purple elephant grass, fermented Guiminyue elephant grass, fermented alfalfa and fermented kudzu and recorded as GG, GR, LM and LA). 48-feathered chickens were treated in each treatment group, with each 4-feathered chicken being housed in the same cage.

Example 2:

in this example, the influence of different fermented feeds in example 1 on the growth performance of the jingling partridge chickens was studied as follows:

1. the experimental animals and the design were carried out according to step 3 of example 1. The indexes of the test period for detecting the growth performance of the Jinling spotted chicken are as follows:

the feed feeding amount and the residual feeding amount in the trough are recorded every day for each group, all test chickens are weighed on an empty stomach, and the daily average weight gain and the feed weight ratio are calculated.

Average daily gain = (end weight-initial weight)/number of days tested

Daily average feed weight ratio = daily average feed intake/daily average gain

2. The results obtained are shown in FIG. 1:

compared with a control group, the daily weight gain of the Holly odorifera test group, the Holly odorifera test group and the Carya illinoensis test group which are added in the full period of the formal period is remarkably reduced (P is less than 0.05, F is not less than 11.079), and the reduction ratios are 14.57%, 14.63% and 5.93% respectively. Wherein the daily average weight gain of the fermented Guijian grassiness test group in the whole period of the official period is reduced most obviously.

Compared with a control group, the fermented purple elephant grass test group is added in the full period of the formal period, the fermented Guijian Xiangcao test group obviously increases the daily average feed weight ratio of the Jinling spotted chickens (P <0.05, F = 7.549), and the increase ratio is respectively 13.70% and 14.59%. Wherein the daily average material weight ratio of the fermented Guijian Xiangcao experimental group in the whole period of the formal period is increased most obviously.

For 10-14 weeks old chickens with the Jinling flowers, the fermented purple elephant grass test group, the fermented Guimin Xiangcao test group, the fermented alfalfa test group and the fermented kudzu vine test group are added, compared with a control group, the daily average weight gain is remarkably reduced (P is less than 0.05, F is not less than 8.929), the reduction ratio is respectively 11.68%, 16.57%, 16.13% and 14.38%, and the fermented Guimin Xiangcao is reduced most remarkably. Compared with a control group, the daily average material weight ratio of the fermented purple elephant grass test group, the fermented Guiminyue elephant grass test group, the fermented alfalfa test group and the fermented kudzu test group is remarkably increased (P is less than 0.05, F is not less than 4.973), the increasing ratios are respectively 10.14%, 17.05%, 16.13% and 14.06%, and the fermented Guiminyue elephant grass is most remarkably increased.

For the Jinling spotted chickens of 15-19 weeks old, the daily average weight gain of the fermented purple elephant grass group and the fermented Guijian Zhen elephant grass group is obviously reduced (P is less than 0.05, F is not less than 7.834) compared with the control group, and the reduction ratio is 17.49 percent and 12.53 percent. And the reduction of the fermented purple elephant grass group is most obvious. Compared with a control group, the daily average material weight ratio of the group added with the fermented purple elephant grass and the fermented GuiMin Zheng elephant grass is remarkably increased (P is less than 0.01, F is less than 7.861), and the increase ratio is 17.66 percent and 11.09 percent. And the rise of the fermented purple elephant grass group is most obvious.

3. The above results show that:

the fermented purple elephant grass, the fermented Guijian Zhenjiao elephant grass and the fermented alfalfa are added, and the fermented kudzu vine reduces the daily average weight gain of the Jinling spotted-brown chickens to different degrees compared with a control group. The fermented purple elephant grass, the fermented Guijian elephant grass and the fermented alfalfa are added, the fermented kudzu increases the daily average feed weight ratio of the Jinling spotted chickens in different degrees compared with a control group, and the daily gain is lower than that of the control group after the fermented alfalfa is added to the Jinling spotted chickens, which is different from the view point that the production performance of animals can be improved by adding alfalfa powder in single-stomach animal diet proposed by the Touris national culture. This may be related to the different dietary formulations and the quality of the different fiber sources. If the microbial mineral content of the feed is sufficient to meet the nutritional needs of the poultry, the addition of a different cellulose source will increase the cellulose content of the feed, reducing the production performance. The addition of fermented alfalfa may adversely affect the performance of chicken production, mainly because alfalfa contains a large amount of high saponins and fibers, and may also be associated with vitamin E analogues, chlorophyllase, isoflavones, and the like. The adverse effects can be effectively relieved after the fermented alfalfa is fed, and the effect is better than that of a group without any treatment. The addition of the purple elephant grass can obviously increase the daily weight gain of the Jinling spotted chickens, while the Guijian elephant grass can reduce the daily average weight gain of the Jinling spotted chickens, but compared with a control group, the fermented elephant grass has better palatability, and has the effects of promoting gastrointestinal motility, improving daily feed intake, accelerating growth speed, shortening marketing time and saving concentrate cost; the daily gain of the Jinling spotted chickens added with the puerarin is lower than that of a control group, the satiety can be improved by fermenting the kudzu, and the palatability of the feed can be influenced by some substances in the puerarin so as to limit the food intake of the feed.

Example 3:

this example investigates the effect of the different fermented feeds of example 1 on the slaughter performance of chickens from the holy gracils.

1. The experimental animals and the design were carried out according to step 3 of example 1, and the slaughter performance test method for the jinling pheasants was as follows:

at the end of the trial, 12 broilers of similar weight were randomly selected from each treatment group for slaughter. Taking each part of the carcass (longissimus dorsi, muscles on two sides of chest and meat on the inner side of waist), and measuring the dry matter content by adopting a weighing method; a sample of the tail fat was taken and its fatty acid value was measured by HPLC. All data were then statistically analyzed. In the test process, slaughter performance indexes such as live body weight, carcass weight, slaughter rate, half-bore rate, full-bore rate, pectoral muscle rate, leg muscle rate, abdominal fat rate and the like are measured. The carcasses and the sampling method are specified in the agricultural industry Standard of the people's republic of China, poultry production performance noun terms and measurement statistical method.

2. The experimental results of the chicken breast muscle slaughtering performance are shown in figure 2:

compared with a control group, the fermented purple elephant grass test group, the fermented Guimin elephant grass test group, the fermented alfalfa test group and the fermented kudzu vine test group are added in the full period of the formal period, the live weight of the Jinling spotted chickens is obviously reduced (P is less than 0.05, F is not greater than 4.746), and the reduction ratio is 15.50%, 7.50%, 11.50% and 11.00% respectively. And the test group added with the fermented purple elephant grass has the most obvious reduction.

Compared with a control group, the fermented purple elephant grass test group, the fermented Guijian Xiangcao test group, the fermented alfalfa test group and the fermented kudzu vine test group are added in the full period of the formal period, the carcasses of Jinling partridge chickens are remarkably reduced (P is less than 0.05, F is not less than 3.973), and the reduction ratios are respectively 16.13%, 9.14%, 11.83% and 11.29%. And the test group added with the fermented purple elephant grass has the most obvious reduction.

Compared with a control group, the slaughtering rate of the jinling partridge in the fermented Guimin introductive elephant grass test group is remarkably reduced (P <0.05, F = 2.408), and the reduction ratio is 1.59 percent respectively. Compared with the control group, the fermented purple elephant grass test group of the Jinling spotted chickens has a significantly reduced abdominal fat rate (P <0.05, F = 2.081) compared with the control group, and the reduction rate is 21.91%. Compared with the control group, the spleen coefficient of the fermented kudzu experimental group of the jinling partridge is remarkably increased (P <0.01, F = 6.384) and the increase rate is 42.86%.

3. The above results show that:

dressing percentage and total mortarless rate are considered as key indicators of chicken dressing performance. The requirement of meat production can be met only when the full-bore-cleaning rate is up to more than 60%. Growth performance, slaughter performance, feed conversion rate, muscle proportion, fat proportion and the like are all related to the feed. In the production of modern broiler chickens, on one hand, the chicken yield is reduced due to feed conversion, and on the other hand, the abdominal fat content is too high due to the development and metabolism disorder; the raising cost can be effectively reduced by reducing the abdominal fat of the broiler chickens. Therefore, it is an important issue to improve meat quality, especially to reduce or control excess abdominal fat. There have been many reports of studies on the effect of dietary fiber on meat quality, but there is still controversy about its specific mechanism. Research by Mohiti-Asli et al shows that when broiler chickens are fed with dietary fibers, addition of a proper amount (3% of cellulose and 8% of inulin) of dietary fibers or inulin helps to reduce abdominal fat content of the broiler chickens, but interaction exists between the cellulose and the inulin. Okrathok et al found that after 0.5%, 1.0% and 1.5% of modified cassava fiber is eaten by broilers, 1% of modified cassava fiber can reduce abdominal fat and cholesterol levels of meat, blood and liver. The test result shows that the addition of the fermented purple elephant grass has no significant influence on the slaughter rate and the full-bore rate of the broiler chickens, and the slaughter rate and the full-bore rate respectively reach more than 91% and 63%, which indicates that the addition of the fermented purple elephant grass does not influence the meat production performance of the broiler chickens. The result shows that the abdominal fat rate is obviously reduced on the basis of not influencing slaughtering performance by adding the fermented purple elephant grass in the daily ration.

Compared with a control group, the addition of the fermented Guimin Wei Xiangcao has obvious influence on the reduction of the broiler slaughter rate, and the complete evisceration rate has no obvious influence, which indicates that the addition of the fermented Guimin Wei Xiangcao influences the meat production performance of the Jinling Huaji. The result shows that the weight of the carcass is obviously increased on the basis that the slaughtering performance is influenced by adding the fermented Guimingxi elephantopus grass in the daily ration; the addition of the fermented alfalfa has no significant influence on the slaughter rate and the full-bore rate of the broiler chicken, and the slaughter rate and the full-bore rate respectively reach more than 91% and 63%, which indicates that the meat production performance of the broiler chicken is not influenced by the addition of the fermented alfalfa; the addition of the fermented kudzu has no significant influence on the slaughtering rate and the full-bore rate of the broiler chickens, and the slaughtering rate and the full-bore rate respectively reach more than 91% and 63%, which indicates that the addition of the fermented kudzu does not influence the meat production performance of the broiler chickens. The result shows that the spleen coefficient is obviously increased on the basis of not influencing slaughtering performance by adding the fermented kudzu in the daily ration.

Example 4:

this example investigates the effect of different fermented feeds of example 1 on the blood physiological indices of chickens from the Jinling.

1. The experimental animals and the design were carried out according to step 3 of example 1, and the blood physiological index test criteria were as follows:

randomly taking 12 chickens from each group, collecting blood of wing vein, centrifuging blood sample in a centrifuge of 2000r/min for 10min, collecting serum, and storing in a refrigerator at-25 deg.C for use. White Blood Cell (WBC) number, red Blood Cell (RBC) number, hemoglobin (HGB) content, hematocrit (HCV), mean Corpuscular Volume (MCV), mean corpuscular hemoglobin content (MCH), and mean corpuscular hemoglobin mass concentration (MCHC) were measured using an XD811 biochemical analyzer (shanghai sudada medical instruments).

2. The results of the experiment on the physiological indexes of the chicken blood are shown in figure 3:

compared with a control group, the white blood cell number of the Jinling spotted chickens is remarkably increased by adding fermented purple elephant grass in the feed (P <0.05, F = 9.646), and the increase rate is 70.39%. Compared with the control group, the white blood cell count of the Jinling partridge chickens is remarkably reduced (P is less than 0.01) by adding the fermented alfalfa into the diet, and the reduction ratio is 90.40%.

Compared with a control group, the average RBC hemoglobin concentration (MCHC) of the Jinling partridge chickens is remarkably reduced (P <0.05, F = 10.671) by adding the fermented Guimin pennisetum purpureum experimental group and the fermented alfalfa experimental group into the feed, and the reduction ratios are 4.91% and 11.53% respectively. The addition of the fermented alfalfa experimental group most remarkably reduces the average RBC hemoglobin concentration (MCHC) of the Jinling spotted chickens.

Compared with a control group, the average red blood cell volume (MCV) of the Jinling spotted deer is remarkably increased (P <0.05, F = 9.646) by adding fermented alfalfa into the diet, and the increase proportion is 21.11%; mean RBC hemoglobin concentration (MCHC) was very significantly reduced from the control group (P <0.01, f = 4.647) with a reduction rate of 11.53%; RBC volume distribution width CV (RDW-CV) was significantly increased (P <0.05, f = 6.819) over the control group with a 50.73% increase; RBC volume distribution width SD (RDW-SD) was significantly increased (P <0.05, f = 5.613) compared to the control group, with an increase ratio of 81.17%; platelet count (PLT) was very significantly increased compared to the control group (P <0.01, f = 15.981) with an increase rate of 600.60%.

Compared with a control group, the addition of the fermented kudzu in the feed has no obvious influence on the physiological indexes of the chicken serum, and the difference among the groups is not obvious (P is more than 0.05).

3. The results of this example show that:

the change of the content of various components in the serum is the result of the function change of animal tissues and organs or the normal metabolism change of substances. These variations can be divided into two categories: one is caused by blood components (e.g., leukocytes); the other is caused by hypovolemia. The factor affecting the ability of red blood cells to generate and release is erythropoietin. Erythrocytes play an important role in serum, not only for the transport of oxygen, but also for the synthesis of hemoglobin. The red blood cells can discharge oxygen and carbon dioxide from the cells, and are combined with water, electrolyte (such as glucose), amino acid and the like to participate in metabolism in vivo; it also has acid-base balance regulating and buffering effects. Both substances contain red blood cells and hemoglobin. Leukocytes play a very important role in the human body as one of the most important immune cells: it is the main defense system in the body; they can phagocytose foreign bodies, produce antibodies, treat body injuries, resist pathogens, and enhance immunity.

The results show that: the fermented purple elephant grass can increase the white blood cell count (P is less than 0.05) of the blood serum of the Jinling spotted chicken, but has the tendency of increasing the disease resistance; the fermented purple elephant grass can slightly reduce the number of serum red blood cells and hemoglobin, thereby reducing the functions of carrying oxygen and delivering oxygen, but has no significance (P > 0.05).

The fermented Guijianxiang elephantopus can improve the number of white blood cells in the serum of the Jinling spotted chicken, but the obvious difference is not obvious (P is more than 0.05), but the white blood cells have the tendency of increasing, so that the disease resistance of the organism is improved. The fermented Chinese angelica, cassia twig and common sage can slightly reduce the number of red blood cells and hemoglobin in blood serum, thereby reducing the functions of carrying oxygen and transporting oxygen, but has no significant difference (P > 0.05). Fermented GuiMin Yinxiao can obviously reduce the average RBC hemoglobin concentration and has obvious difference (P < 0.05).

The fermented alfalfa can obviously reduce the number of leucocytes in the serum of the Jinling partridge, and has obvious difference (P is less than 0.05), thereby reducing the disease resistance of organisms. Fermented alfalfa significantly reduces red blood cells in serum. Fermentation of alfalfa significantly increased serum red blood cells, mean red blood cell volume, RBC volume distribution width CV, RBC volume distribution width SD, platelet count, and significant differences (P < 0.05). Fermentation of alfalfa decreased mean RBC hemoglobin very significantly, with very significant differences (P < 0.01).

The fermented kudzu vine can increase the number of leucocytes in the serum of the Jinling partridge chicken, but the significant difference is not significant (P is more than 0.05), but the variation is increased, so that the disease resistance of the organism is improved. The fermentation of kudzu vine slightly reduces the number of red blood cells and hemoglobin in serum, thereby reducing the functions of oxygen carrying and transporting, but has no significant difference (P > 0.05). Therefore, different fiber sources added into the daily ration have different influences on the blood physiological indexes of the Jinling spotted chickens.

From examples 2 to 4, the following conclusions can be drawn: (1) The growth performance of the Jinling partridge chicken is remarkably reduced by adding fermented purple elephant grass, fermented Guijian peng elephant grass, fermented alfalfa and fermented kudzu; in the full period of the formal period, the initial weight and the final weight of the Jinling spotted chickens added with the fermented purple elephant grass experimental group are reduced most obviously; the daily average weight gain of the Jinling spotted chickens added with the fermented Guijian Xiangcao experimental group is reduced most obviously; the daily average feed weight ratio of the Jinling spotted chickens added with the fermented Guijian Xiangcao experimental group is increased most obviously; when the chickens are 10-14 weeks old, the daily average weight gain of the chickens in the Jinling Huaji added with the fermented Guijian Xiangcao experimental group is reduced most obviously; the daily average feed weight ratio of the Jinling spotted chickens added with the fermented Guijian Xiangcao experimental group is increased most obviously; compared with a control group at the age of 15-19 weeks, the average daily gain of the Jinling spotted chickens added with the fermented purple elephant grass experimental group is reduced most obviously; the daily average material weight ratio of the experimental group added with the fermented purple elephant grass is increased most remarkably; (2) The slaughtering performance of the Jinling spotted chickens can be obviously influenced by adding fermented purple elephant grass, fermented Guijian peng elephant grass, fermented alfalfa and fermented kudzu, and the live weight and the carcass weight can be obviously reduced; the live weight and carcass weight of the Jinling spotted chickens added with the fermented purple elephant grass test group are reduced most obviously; the slaughtering rate of the Jinling spotted chickens in the test group of adding the fermented Guijian Xiangcao is obviously reduced; the abdominal fat rate of the Jinling spotted chickens added with the fermented purple elephant grass test group is obviously reduced; the spleen coefficient of the Jinling flower chicken added with the fermented kudzu experimental group is remarkably increased; (3) The blood physiological indexes of the Jinling spotted chickens can be obviously influenced by adding the fermented purple elephant grass, the fermented Guijian peng elephant grass and the fermented alfalfa, but the influence of the experimental group added with the fermented kudzu vine on the blood physiological indexes of the Jinling spotted chickens is not obvious. Compared with a control group, the white blood cell count of the Jinling partridge chickens is obviously increased by adding the fermented purple elephant grass; the white blood cell number of the Jinling spotted chickens is remarkably reduced by adding the fermented alfalfa into the feed; the addition of the fermented alfalfa experimental group most obviously reduces the average RBC hemoglobin concentration (MCHC) of the Jinling spotted chickens; the average red blood cell volume (MCV) of the Jinling spotted chickens is obviously increased by adding the fermented alfalfa into the feed; mean RBC hemoglobin concentration (MCHC) is very significantly reduced; RBC volume distribution width CV (RDW-CV) is significantly increased; RBC volume distribution width SD (RDW-SD) is significantly increased; platelet count (PLT) was very significantly increased.

Data statistics for examples 2-4 single factor analysis of variance was performed on each index data using SPSS 23.0. The test data are expressed as "mean ± sem". The significance of the differences between groups was compared by the Least Significant Difference (LSD) method. P <0.01 indicates that the difference is very significant, P <0.05 indicates that the difference is significant, and P >0.05 indicates that the difference is not significant.

Example 5:

the present example mainly studies the influence of different fermentation fiber source combinations on the growth of jinling spotted maigre chickens, which is as follows:

from the studies of examples 2-4, we know that: the above several fermented fiber sources have a certain effect of reducing the growth performance of the chickens suffering from the jingling flower, but in the experiment, it is found that if different fiber sources are used in combination, the fermented fiber sources have a certain effect of promoting the growth of the chickens suffering from the jingling flower, which is completely different from the research result of the embodiment 2, therefore, the embodiment adopts different fiber source combinations to research the growth of the chickens suffering from the jingling flower, and specifically comprises the following steps:

a random block experiment design is adopted, 1 day old Jinling spotted chickens are selected, healthy Jinling spotted chickens without obvious weight difference are selected, and the Jinling spotted chickens are randomly divided into 12 experiment groups. One group served as a control group (basal formula feed, denoted CK) and the remaining four groups were fed with fermented forage separately from the first week of age and in place of the basal diet (the types of fermented forage added are shown in groups 1-11 of table 2). 48-feathered chickens were treated in each treatment group, with each 4-feathered chicken being housed in the same cage.

TABLE 2 different fermented forage grass experimental groups

Group of	Mass ratio of fermented forage grass
		Group 1	Mixing fermented purple elephant grass with fermented Guimin Zhenjiao grass according to a ratio of 1
Group 2	Mixing fermented purple elephant grass and fermented kudzu according to the ratio of 1
		Group 3	Mixing fermented purple elephant grass with fermented alfalfa according to the proportion of 1
Group 4	Mixing fermented Guijianxiaohui and fermented kudzu according to the ratio of 1
		Group 5	Mixing fermented GuiMin Xiangxiang grasses and fermented alfalfa according to the proportion of 1
Group 6	Mixing the fermented alfalfa and the fermented kudzu according to the proportion of 1
		Group 7	Mixing fermented purple elephant grass, fermented Guimin elephant grass and fermented kudzu vine according to a ratio of 1
Group 8	1, mixing fermented purple elephant grass, fermented Guimin elephant grass and fermented alfalfa
		Group 9	Mixing fermented purple elephant grass, fermented alfalfa and fermented kudzu according to a ratio of 1
Group 10	Mixing fermented GuiMin Daiwei grasses, fermented alfalfa and fermented kudzu according to a ratio of 1
		Group 11	The fermented purple elephant grass, the fermented Guimin elephant grass, the fermented alfalfa and the fermented kudzu are mixed according to the ratio of 1

The feed feeding amount and the residual feeding amount in the trough are recorded every day for each group, all test chickens are weighed on an empty stomach, and the daily average weight gain, the feed weight ratio and the initial weight and the final weight are calculated.

Average daily gain = (end weight-initial weight)/number of days tested

Daily average feed weight ratio = daily average feed intake/daily average gain

The results obtained are shown in table 3:

TABLE 3 influence of different groups of forage on the performance of chickens from Jinling

As can be seen from table 3, the combination of the forage grasses from multiple plant sources has both promoting effect and crop inhibition compared with the effect of the forage grass from a single source on the growth performance of the jingling partridge chickens, and the experimental results show that the daily weight gain of the jingling partridge chickens can be significantly improved compared with that of the CK pigs by the forage grass proportion of the group 9 and the group 10, the daily weight gain of the jingling partridge chickens can be reduced to different degrees compared with that of the CK pigs by other experimental groups, and the feed weight ratio of the group 9 is lower than that of the group 10 in terms of the feed weight ratio; therefore, from the viewpoint of feed utilization, it is optimal to select group 9 mixed forage as a fiber source feed for the Jinling broiler. That is, it should be preferable that: adding fermented forage grass mixed with fermented purple elephant grass, fermented alfalfa and fermented kudzu in a mass ratio of 1.

Example 6:

in this example, the optimum ratio of the fermented fiber source feed composition is studied, specifically as follows:

designing an orthogonal experiment according to the mass ratio of the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu vine, feeding the Jinling partridge chickens in groups by adopting the method of the embodiment 5, and taking the daily gain of 10-14 weeks as a screening index; the method comprises the following specific steps:

TABLE 4 levels of orthogonal experimental factors for different fermented forage grasses

The results of the orthogonal analysis of the above formulation are shown in Table 5:

TABLE 5 Quadrature experiments and results

Number of tests	Fermented purple elephant grass	Fermented alfalfa	Fermented kudzu vine	Daily average weight gain (g/d)
					1	A	A	A	19.2
2	A	B	B	19.5
					3	A	C	C	21.3
4	B	A	B	18.1
					5	B	B	C	17.3
6	B	C	A	18.6
					7	C	A	C	16.8
8	C	B	A	15.1
					9	C	C	B	17.6
r1	20.000	18.0333	17.633
					r2	18.000	17.300	18.400
r3	16.500	19.167	18.467
					R	3.500	1.867	0.834

As can be seen from the above table, the daily average weight gain in the experiment of group 3 is the highest, and reaches 21.3g/d, namely, the purple elephant grass is fermented: fermenting alfalfa: the mass ratio of the fermented kudzu is 1; except that the differences between the groups 7-9 and the CK group in the example 5 are not significant, the fermented forage grass combination of other groups can effectively increase the daily weight gain of the Jinling spotted chickens, which indicates that the fermented purple grass: fermenting alfalfa: the weight gain of the Jinling spotted chickens can be effectively improved by the mass ratio of the fermented kudzu to 1. From the extremely poor point, the fermented purple elephant grass is more than the fermented alfalfa is more than the fermented kudzu, thereby showing that the fermented purple elephant grass has the greatest influence on the daily gain of the Jinling spotted chicken.

Example 7:

this example studies the effect of fermented forage combinations on slaughter performance and blood performance of chickens from the daling.

From the studies of examples 3 and 4, it was found that the difference in the effects of fermented forage grass mainly on live body weight, carcass weight, abdominal fat percentage, spleen coefficient and white blood cells is significant, and therefore, in this example, the effects of the fermented fiber combination on the above-mentioned several indexes were mainly studied, specifically as follows:

the best group (group 3) from example 6 was selected and labeled as experimental group 1, the worst group (group 8) was labeled as experimental group 2, and the CK group without fermented fiber forage was compared in animal experiments, and the experimental design was performed as described in example 1, and the test methods were described in example 3 and example 4, and the results are shown in table 6.

TABLE 6 influence of different experimental groups on the muscle performance and blood index of the chickens with Jinling flowers

Group of	Live body weight (kg)	Carcass weight (kg)	Abdominal fat percentage (%)	Spleen factor (%)	White blood cell count (WBC)
						Experimental group 1	2.3	2.0	4.8	2.1	91
Experimental group 2	1.6	1.3	4.9	2.0	87
						CK group	1.8	1.5	6.1	0.13	53

As can be seen from table 6, the live weights and carcass weights of the jingling chickens in experimental group 1 were significantly higher than those of experimental group 2 and CK group, while the live weights and carcass weights of the jingling chickens in experimental group 2 were lower than those of CK group, indicating that after the fermented forage grass of experimental group 1 was used in combination, and fermented purple elephant grass: fermenting alfalfa: the fermented kudzu vine has the mass ratio of 1; from the perspective of abdominal fat rate, spleen coefficient and white blood cell count in blood, the abdominal fat rate of the experimental group 1 and the experimental group 2 is obviously lower than that of the CK group, and the spleen coefficient and the white blood cell count are obviously higher than that of the CK group, so that the fermented forage grass can effectively reduce the abdominal fat rate of the Jinling flower chicken, the chicken is firmer, the fat is less, the spleen coefficient and the white blood cell count in blood are obviously higher than that of the CK group, and the fermented forage grass can obviously improve the immunity of the Jinling flower chicken.

The fermented feed of the experimental examples 1 to 7 was prepared as follows:

1. preparing fermented forage grass:

removing impurities from the purple elephant grass, the guijian elephant grass, the alfalfa and the kudzu vine respectively, cleaning, cutting into pieces by a hay cutter, adding a silage micro-storage agent purchased from the market according to the instruction, putting into a scenting pool for composting, and taking out a fermentation product after 25-30 days to obtain the corresponding fermented forage grass.

The silage micro-storage agent (purchased from Yichun Strong microorganism science and technology Co., ltd.) of the embodiment comprises Candida utilis, bacillus subtilis and Pediococcus acidilactici.

2. Preparing fermented feed:

weighing each raw material according to the proportion in the table 1 of the application, retting the raw materials with corresponding fermented forage grass for 20-25d after fully mixing, taking out the retted product, air-drying, crushing and granulating to prepare the silage.

In conclusion, a large number of animal experiments prove that the fermented forage grass has a remarkable effect on the growth of the Jinling spotted chickens, and the fermented forage grass has no remarkable effect on the weight increase of the Rilingspotted chickens from the perspective of the forage grass of a single fiber source, and the weight of the Jinling spotted chickens can be remarkably increased after compounding, which indicates that the single fiber source forage grass has single nutrient content and cannot meet the growth requirement of the Jinling spotted chickens, and the fermented forage grass can remarkably increase the weight of the Jinling spotted chickens after compounding improvement and formula optimization, and the comparative experiments in the later period also find that different fiber sources: after the forage grass of the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu vine is used in combination, the abdominal fat rate of the Jinling spotted chickens can be obviously reduced, the leukocyte content and the spleen coefficient in blood are improved, and the forage grass is rare excellent fermented forage grass.

The above examples are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The broiler silage is characterized by comprising the following raw materials in percentage by weight: 57.52 percent of corn, 22.05 percent of soybean meal, 8.73 percent of wheat bran, 2.3 percent of fish meal, 1.4 percent of stone powder and 8 percent of fermented forage grass; the fermented forage grass consists of fermented purple elephant grass, fermented alfalfa and fermented kudzu.

2. The broiler silage as claimed in claim 1, wherein the mass ratio of fermented purple elephant grass, fermented alfalfa and fermented kudzu in the fermented forage grass is 1.

3. The broiler silage according to claim 1 or 2, wherein the mass ratio of the fermented purple elephant grass, the fermented alfalfa and the fermented kudzu in the fermented forage grass is 1.

4. Use of the broiler silage of claim 1 or 2 for increasing the weight and/or reducing the abdominal fat rate of a jinling Huaji.

5. A method for preparing silage for broiler chickens as claimed in claim 1 or 2, characterized in that the method is as follows:

6. A method for feeding Jinling Huaji with the broiler silage of claim 1 or 2, wherein the method is characterized in that the method comprises the step of feeding the Jinling Huaji which is just born with a basic feed without adding fermented forage grass, and after 1 week of age, the broiler silage with the fermented forage grass is used for feeding instead of the basic feed, and the broiler silage is prepared by mixing fermented purple elephant grass, fermented alfalfa and fermented kudzu according to the mass ratio of 1.