CN115612617A

CN115612617A - Biological vaccine multicellular reactor

Info

Publication number: CN115612617A
Application number: CN202211552868.3A
Authority: CN
Inventors: 安有才; 陈则; 徐奇; 游媛媛
Original assignee: Jiangsu Zhonghui Yuantong Biotechnology Co ltd
Current assignee: Jiangsu Zhonghui Yuantong Biotechnology Co ltd
Priority date: 2022-12-06
Filing date: 2022-12-06
Publication date: 2023-01-17

Abstract

The invention relates to the technical field of biological vaccines, in particular to a biological vaccine multicellular reactor which comprises a multicellular generator and a sealing cover arranged on the multicellular generator, wherein one end of the multicellular generator is provided with a connecting through hole, a fixed limiting plate is welded on the multicellular generator at one side of the connecting through hole, a matching groove is formed in the fixed limiting plate, a mounting hole is formed in the multicellular generator at one side of the fixed limiting plate, the other end of the multicellular generator is fixedly provided with a vent pipe, and an inner end surface, opposite to the vent pipe, of the inside of the multicellular generator is provided with an inner shrinkage jack.

Description

Biological vaccine multicellular reactor

Technical Field

The invention relates to the technical field of biological vaccines, in particular to a biological vaccine multicellular reactor.

Background

The development of biological vaccines needs to culture cells, the culture of the cells needs to be carried out in a multi-cell reactor, the cells need to be aerated into the multi-cell reactor in the culture process to ensure the normal physiological activity of the cells, the traditional aeration mode is to directly aerate air into a culture solution, so that air bubbles are generated and are broken in the culture solution, the cells are fragile and very easy to cause cell death, in addition, in order to increase the oxygen content in the culture solution, the culture solution is stirred in a stirring mode, and the shearing force generated by blades during the stirring of the culture solution also very easily causes the cell death, so that the survival rate of the cells is not high.

Disclosure of Invention

The present invention is directed to a biological vaccine multi-cell reactor to solve the above problems of the background art.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a biological bacterin multicellular reactor, includes multicellular generator and installs the sealed lid on multicellular generator, connect the through hole has been seted up to the one end of multicellular generator, the welding has fixed limiting plate on the multicellular generator of connect the through hole one side, the cooperation groove has been seted up on the fixed limiting plate, and has seted up the mounting hole on the multicellular generator of fixed limiting plate one side, the other end of multicellular generator is fixed and is provided with the breather pipe, and has seted up the jack-in on the inside relative interior terminal surface with the breather pipe of multicellular generator, the jack-in link up with connect the through hole, and the welding has first support guide pillar on the inner wall of the multicellular generator of jack-in downside has the stopper, the welding has the supporting shoe on the multicellular generator of the inner port downside of breather pipe, the cooperation guide hole has been seted up on the supporting shoe, and the symmetry welding has the second support guide pillar on the multicellular generator's of supporting shoe lower extreme inner wall, install on the multicellular generator and can push the air in the culture solution and push down the mechanism.

Preferably, the air pressing mechanism comprises a piston air pressure plate, a rotating long shaft, a movable supporting block, a first pushing plate, a movable block and a second pushing plate, the piston air pressure plate is inserted into the multi-cell generator, a pressing column is welded at the upper end of the piston air pressure plate, and the pressing column penetrates through the sealing cover.

Preferably, the upper end of the pressing column is welded with a pressing plate, one side of the pressing plate is welded with a side support plate, the end of the side support plate is welded with a rack, the rack faces downwards vertically, the pressing column is sleeved with a first spring, and the first spring is located at the upper end of the sealing cover.

Preferably, the rotating long shaft is sleeved with a bearing and is installed in the installation hole through the bearing, a fixed plate is welded on one side of the rotating long shaft, an annular cylindrical rod is welded on the fixed plate, an annular spring is sleeved on the annular cylindrical rod, and the annular cylindrical rod is partially inserted into the matching groove.

Preferably, the other end of the rotating long shaft is fixedly provided with a half-tooth gear, the half-tooth gear is meshed with the rack, and an inclined annular top plate is fixedly arranged on the end face, close to the multicellular generator, of the half-tooth gear.

Preferably, the movable supporting block is inserted into the inward-shrinkage jack, the rear end of the movable supporting block is welded with the connecting inserted rod, the connecting inserted rod is inserted into the connecting through hole, the connecting inserted rod is sleeved with the second spring, the end of the connecting inserted rod is welded with the matching block, the upper end of the matching block is movably provided with the rotating wheel, and the rotating wheel is in contact with the inclined plane circular ring top plate.

Preferably, a first connecting plate is welded in the middle of the first pushing plate, a guide jack is formed in the first connecting plate, a first supporting guide pillar is inserted into the guide jack, and a third spring is arranged between the first connecting plate and the limiting block and sleeved on the first supporting guide pillar.

Preferably, the lower extreme welding of movable block has the guide bar, the guide bar is pegged graft in the cooperation guide hole, and has cup jointed the fourth spring on the guide bar, the movable block is in the upside of supporting shoe, and articulates on the movable block has movable hinge rod, the lower extreme of movable hinge rod articulates on the second connecting plate, the second connecting plate is fixed to be set up on the intermediate position of second slurcam, the both ends symmetry of second slurcam is fixed and is provided with the deflector, the direction cooperation through-hole has been seted up on the deflector, the second support guide pillar has been pegged graft in the direction cooperation through-hole.

Compared with the prior art, the invention has the beneficial effects that: the invention has reasonable structure and strong functionality, and has the following advantages:

1. change the mode that traditional direct was ventilated in toward the culture solution, set up the breather pipe in the upper end of culture solution, let in the multicellular generator with the air, then make the inside pressure intensity grow of multicellular generator through the mode of down pressing piston gas-pressure plate, and then make the air can be merged into the culture solution, can not produce the bubble, and then can not cause the death of cell.

2. The piston gas pressure plate can drive the half-tooth gear to rotate in the moving process, the movable supporting block can be pushed to move under the action of the inclined ring top plate on the half-tooth gear, the piston gas pressure plate can be limited, and the phenomenon that the piston gas pressure plate moves down too much due to misoperation, so that the air pressure in the multi-cell generator is too high, and cell death is caused.

3. In addition, the movable supporting block can drive the first pushing plate to move in the moving process, the piston air pressure plate can drive the second pushing plate to move in the moving process, the culture solution can be pushed to move under the action of the two pushing plates, and the culture solution is in a dynamic state, so that air is easily blended into the culture solution, and the oxygen content in the culture solution is increased while cells are not damaged.

Drawings

FIG. 1 is a schematic view of the assembly of the multicellular generator.

Fig. 2 is an exploded view of the internal assembly of the multicellular generator.

FIG. 3 is a schematic diagram of the external structure of the multicellular generator.

Fig. 4 is a schematic diagram of the internal structure of the multicellular generator.

Fig. 5 is an enlarged schematic view of a point a in fig. 1.

Fig. 6 is a schematic view of a structure of a rotating long shaft.

In the figure: 1. a multicellular generator; 11. a connecting through hole; 12. fixing a limit plate; 13. a mating groove; 14. mounting holes; 15. a breather pipe; 16. an inward shrinkage jack; 17. a first support guide post; 171. a limiting block; 18. a support block; 19. a second support guide post; 2. a sealing cover; 3. a piston gas pressure plate; 31. pressing the column; 32. a pressing plate; 33. a side support plate; 34. a rack; 35. a first spring; 4. rotating the long shaft; 41. a fixing plate; 42. an annular cylindrical rod; 43. an annular spring; 44. a half-tooth gear; 45. a bevel ring top plate; 5. a movable supporting block; 51. connecting the inserted link; 52. a second spring; 53. a matching block; 54. a rotating wheel; 6. a first push plate; 61. a first connecting plate; 62. a third spring; 7. a movable block; 71. a guide rod; 72. a fourth spring; 73. a movable hinge rod; 8. a second pusher plate; 81. a second connecting plate; 82. a guide plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 6, the present invention provides a technical solution:

the utility model provides a biological bacterin multicellular reactor, including multicellular generator 1 and the sealed lid 2 of installing on multicellular generator 1, connect through-hole 11 has been seted up to the one end of multicellular generator 1, it has fixed limiting plate 12 to weld on the multicellular generator 1 of connect through-hole 11 one side, cooperation groove 13 has been seted up on fixed limiting plate 12, and seted up mounting hole 14 on the multicellular generator 1 of fixed limiting plate 12 one side, the other end of multicellular generator 1 is fixed and is provided with breather pipe 15, and the inside of multicellular generator 1 has seted up interior jack 16 on the inner terminal surface relative with breather pipe 15, interior jack 16 has been link up with connect through-hole 11, and weld on the inner wall of multicellular generator 1 of interior jack 16 downside has first support guide pillar 17, the tip welding of first support guide pillar 17 has stopper 171, the welding has 18 on the multicellular generator 1 of the inner mouth downside of 15, the cooperation guide hole has been seted up on the support shoe 18, and the symmetry welding has second support pillar 19 on the multicellular generator 1's 1 inner wall of support shoe 18 lower extreme, install on the multicellular generator 1 can push down the air in the culture fluid, and push down the air pressure mechanism 3 pushes down and push plate 3 push plates 3, the piston push plate 2 push plate 3 is 3 and push plate 3, the piston push plate 3 is 3 and push plate 3 is the piston push plate 3 and push plate 3 is matched with the piston push plate mechanism and the piston push plate 3 is matched with the piston push plate 3, the piston push plate 3 is matched.

The upper end of the pressing column 31 is welded with the pressing plate 32, one side of the pressing plate 32 is welded with the side support plate 33, the end of the side support plate 33 is welded with the rack 34, the rack 34 faces downwards vertically, the pressing column 31 is sleeved with the first spring 35, the first spring 35 is located at the upper end of the sealing cover 2, and two ends of the first spring 35 are respectively welded on the upper end face of the sealing cover 2 and the lower end face of the pressing plate 32.

The bearing is sleeved on the rotary long shaft 4 and is installed in the installation hole 14 through the bearing, a fixing plate 41 is welded on one side of the rotary long shaft 4, an annular cylindrical rod 42 is welded on the fixing plate 41, an annular spring 43 is sleeved on the annular cylindrical rod 42, part of the annular cylindrical rod 42 is inserted into the matching groove 13, the width of the matching groove 13 is equal to the diameter of the annular cylindrical rod 42, two ends of the annular spring 43 are respectively welded on the fixing plate 41 and the fixed limiting plate 12, a half-tooth gear 44 is fixedly arranged at the other end of the rotary long shaft 4, the half-tooth gear 44 is meshed with the rack 34, an inclined ring top plate 45 is fixedly arranged on the end face, close to the multicellular generator 1, of the half-tooth gear 44, and the surface of the inclined ring top plate 45 is smooth and free of burrs.

The movable supporting block 5 is inserted in the inward shrinkage jack 16, the rear end of the movable supporting block 5 is welded with the connecting inserted bar 51, the connecting inserted bar 51 is inserted in the connecting through hole 11, the connecting inserted bar 51 is sleeved with the second spring 52, the end part of the connecting inserted bar is welded with the matching block 53, the second spring 52 is positioned outside the multicellular generator 1, two ends of the second spring 52 are respectively contacted with the matching block 53 and the outer wall of the multicellular generator 1, the upper end of the matching block 53 is movably provided with the rotating wheel 54, and the rotating wheel 54 is contacted with the inclined plane circular ring top plate 45.

The middle of the first pushing plate 6 is welded with a first connecting plate 61, a guide insertion hole is formed in the first connecting plate 61, a first support guide pillar 17 is inserted in the guide insertion hole, a third spring 62 is arranged between the first connecting plate 61 and the limiting block 171, the third spring 62 is sleeved on the first support guide pillar 17, two ends of the third spring 62 are respectively in contact with the limiting block 171 and the first connecting plate 61, the first connecting plate 61 is partially in contact with the movable supporting block 5, and the first pushing plate 6 is located in the culture solution.

The lower extreme welding of movable block 7 has guide bar 71, guide bar 71 pegs graft in the cooperation guide hole, and cup jointed fourth spring 72 on the guide bar 71, movable block 7 is in the upside of supporting shoe 18, fourth spring 72 is in the upside of supporting shoe 18, and the both ends of fourth spring 72 weld respectively on the lower terminal surface of movable block 7 and on the up end of supporting shoe 18, and it has movable hinge pole 73 to articulate on the movable block 7, the lower extreme of movable hinge pole 73 articulates on second connecting plate 81, second connecting plate 81 is fixed to be set up on the intermediate position of second catch flap 8, the both ends symmetry of second catch flap 8 is fixed and is provided with deflector 82, the direction cooperation through-hole has been seted up on deflector 82, peg graft in the direction cooperation through-hole and have second support guide pillar 19, second catch flap 8 is in addition in the culture solution.

The position of the vent pipe 15 is at the upper end of the culture solution, after the ventilation is completed, the pressing plate 32 can be pressed downwards, so that the piston air pressure plate 3 can be driven to move downwards, and further the pressure inside the multicellular generator 1 is increased, so that air can be partially merged into the culture solution, in the process that the piston air pressure plate 3 moves downwards, the rack 34 is meshed with the half-tooth gear 44 to drive the half-tooth gear 44 to rotate, and further the matching block 53 is pushed towards the direction of the multicellular generator 1 under the action of the inclined ring top plate 45, and simultaneously the movable supporting block 5 is driven to extend out of the inner shrinkage hole 16, so that the movable supporting block 5 can play a limiting role on the piston air pressure plate 3, thereby avoiding that the piston air pressure plate 3 moves downwards too much due to misoperation, causing damage to cells due to overlarge internal air pressure of the multicellular generator 1, and in the process that the movable supporting block 5 pushes the first pushing plate 6 to move, so that the first pushing plate 6 pushes the culture solution, and further causes damage to the second movable supporting block 7, and further pushing the second movable plate 8 can not move downwards, and further pushing the second movable supporting block 7 can not cause damage to the second cell culture solution, and further pushing the second cell pushing plate 8, the survival rate of cell has been improved greatly, release pressing plate 32 can make piston pressure panel 3 realize restoring to the throne under first spring 35's effect in addition, and then can drive half-tooth gear 44 and reverse, and then realize movable supporting shoe 5, first slurcam 6, the reseing of movable block 7 and second slurcam 8, the ring spring 43 of setting on rotating major axis 4 guarantees when piston pressure panel 3 takes off from multicellular generator 1, half-tooth gear 44 can guarantee stable state, random rotation can not take place.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A biological vaccine multicellular reactor, comprising a multicellular generator (1) and a sealing cover (2) mounted on the multicellular generator (1), characterized in that: connecting hole (11) have been seted up to the one end of multicellular generator (1), the welding has fixed limiting plate (12) on multicellular generator (1) of connecting hole (11) one side, cooperation groove (13) have been seted up on fixed limiting plate (12), and has seted up mounting hole (14) on the multicellular generator (1) of fixed limiting plate (12) one side, the other end of multicellular generator (1) is fixed and is provided with breather pipe (15), and has seted up interior jack (16) on the inside relative interior terminal surface with breather pipe (15) of multicellular generator (1), interior jack (16) link up with connecting hole (11), and has welded first support guide pillar (17) on the inner wall of multicellular generator (1) of interior jack (16) downside, the tip welding of first support guide pillar (17) has stopper (171), the welding has supporting shoe (18) on multicellular generator (1) of the interior port downside of breather pipe (15), set up cooperation guide hole on supporting shoe (18), and the symmetry welding has support pillar (19) on the inner wall of multicellular generator (1) of supporting shoe (18) lower extreme has the symmetry to press the air culture mechanism on the multicellular generator (1), air culture mechanism.

2. A biological vaccine multicellular reactor as recited in claim 1, wherein: the air pressing mechanism comprises a piston air pressure plate (3), a rotating long shaft (4), a movable supporting block (5), a first pushing plate (6), a movable block (7) and a second pushing plate (8), the piston air pressure plate (3) is inserted into the multi-cell generator (1), a pressing column (31) is welded at the upper end of the piston air pressure plate (3), and the pressing column (31) penetrates through a sealing cover (2).

3. A biological vaccine multicellular reactor as recited in claim 2, wherein: the upper end of pressing post (31) is welded with pressing plate (32), one side of pressing plate (32) is welded with side support plate (33), the end of side support plate (33) is welded with rack (34), rack (34) vertically faces downwards, pressing post (31) is sleeved with first spring (35), and first spring (35) is located at the upper end of sealing cover (2).

4. A biological vaccine multicellular reactor as recited in claim 2, wherein: the rotary long shaft (4) is sleeved with a bearing and is installed in the installation hole (14) through the bearing, a fixing plate (41) is welded on one side of the rotary long shaft (4), an annular cylindrical rod (42) is welded on the fixing plate (41), an annular spring (43) is sleeved on the annular cylindrical rod (42), and part of the annular cylindrical rod (42) is inserted into the matching groove (13).

5. A biological vaccine multi-cell reactor as claimed in claim 4, characterised in that: the other end of the rotating long shaft (4) is fixedly provided with a half-tooth gear (44), the half-tooth gear (44) is meshed with the rack (34), and an inclined ring top plate (45) is fixedly arranged on the end face, close to the multicellular generator (1), of the half-tooth gear (44).

6. A biological vaccine multicellular reactor as recited in claim 2, wherein: the movable supporting block (5) is inserted into the inward-shrinkage jack (16), the rear end of the movable supporting block (5) is welded with the connecting inserted bar (51), the connecting inserted bar (51) is inserted into the connecting through hole (11), the connecting inserted bar (51) is sleeved with the second spring (52), the end of the connecting inserted bar is welded with the matching block (53), the upper end of the matching block (53) is movably provided with the rotating wheel (54), and the rotating wheel (54) is in contact with the inclined plane circular ring top plate (45).

7. A biological vaccine multicellular reactor as recited in claim 2, wherein: a first connecting plate (61) is welded in the middle of the first pushing plate (6), a guide inserting hole is formed in the first connecting plate (61), a first supporting guide pillar (17) is inserted into the guide inserting hole, a third spring (62) is arranged between the first connecting plate (61) and the limiting block (171), and the third spring (62) is sleeved on the first supporting guide pillar (17).

8. A biological vaccine multicellular reactor as recited in claim 2, wherein: the lower extreme welding of movable block (7) has guide bar (71), guide bar (71) are pegged graft in the cooperation guide hole, and have cup jointed fourth spring (72) on guide bar (71), movable block (7) are in the upside of supporting shoe (18), and articulated on movable block (7) have movable hinge pole (73), the lower extreme of activity hinge pole (73) articulates on second connecting plate (81), second connecting plate (81) are fixed to be set up on the intermediate position of second slurcam (8), both ends symmetry of second slurcam (8) are fixed and are provided with deflector (82), direction cooperation through-hole has been seted up on deflector (82), second support guide pillar (19) have been pegged graft in the direction cooperation through-hole.