CN209989410U - Stem cell culture state monitor - Google Patents
Stem cell culture state monitor Download PDFInfo
- Publication number
- CN209989410U CN209989410U CN201920082696.5U CN201920082696U CN209989410U CN 209989410 U CN209989410 U CN 209989410U CN 201920082696 U CN201920082696 U CN 201920082696U CN 209989410 U CN209989410 U CN 209989410U
- Authority
- CN
- China
- Prior art keywords
- case lid
- stem cell
- cell culture
- box
- state monitor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 29
- 238000004113 cell culture Methods 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000002184 metal Substances 0.000 claims abstract description 14
- 239000000523 sample Substances 0.000 claims description 16
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 abstract description 9
- 239000001301 oxygen Substances 0.000 abstract description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 5
- 239000001569 carbon dioxide Substances 0.000 abstract description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract description 2
- 235000017491 Bambusa tulda Nutrition 0.000 abstract description 2
- 241001330002 Bambuseae Species 0.000 abstract description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract description 2
- 239000011425 bamboo Substances 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000252254 Catostomidae Species 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the biotechnology field, more specifically, a stem cell culture state monitor, including box and case lid, the inside of case lid is equipped with first telescopic machanism, second telescopic machanism, the lifter plate is installed to the lower extreme of first telescopic machanism, install electrode sensor on the lifter plate, the surface mounting of case lid has the inflater, the inside of inflater is equipped with the piston board, run through the tubular metal resonator on the piston board, the surface mounting of case lid has the control box, the left side of control box is equipped with the leading water pipe, the left side wall detachably of case lid installs the collecting box, through setting up liftable electrode sensor, dissolved oxygen content, pH value, carbon dioxide content in the culture solution are monitored in real time, through setting up the piston section of thick bamboo, can inject the air into this device under the condition of not opening the case lid, increase the oxygen content in the culture solution, through setting up leading water pipe and collecting box, can conveniently and quickly finish the sampling operation.
Description
Technical Field
The utility model relates to the field of biotechnology, the more specifically stem cell culture state monitor that says so.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent (multipotent). Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field.
The culture process of stem cells is often carried out in a culture dish, the culture dish has a single function, the culture state of the stem cells cannot be monitored in real time, and the requirements of people are difficult to meet.
Disclosure of Invention
An object of the utility model is to provide a stem cell culture state monitor to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a stem cell culture state monitor, includes box and case lid, the inside of case lid is equipped with first telescopic machanism, second telescopic machanism, and the lifter plate is installed to first telescopic machanism's lower extreme, installs electrode sensor on the lifter plate, and the last surface mounting of case lid has the inflater, and the inside of inflater is equipped with the piston board, and it has the tubular metal resonator to run through on the piston board, and the last surface mounting of case lid has the control box, and the left side of control box is equipped with the leading water pipe, and the collecting box is installed to the left side wall detachably.
Further: the lower end of the second telescopic mechanism is provided with an electron microscope probe, the upper surface of the control box is provided with an electron microscope, and the electron microscope probe is electrically connected with the electron microscope through a telescopic lead.
Further: and the bottom of the box body is provided with support legs.
Further: and the lower ends of the supporting legs are provided with suckers.
Further: the lateral wall of case lid is installed movable buckle, and the top of movable buckle is equipped with the handle.
Adopt the technical scheme provided by the utility model, compare with prior art, have following beneficial effect: through setting up the electrode sensor of liftable, dissolved oxygen content, pH value, carbon dioxide content to in the culture solution monitor in real time, through setting up a piston section of thick bamboo, can be under the condition of not opening the case lid to this device in inject the air, increase the oxygen content in the culture solution, through setting up leading water pipe and collecting box, can accomplish the sample operation convenient and fast, solved current stem cell culture device and can't monitor, and the inconvenient problem of culture solution sample to the process of cultivateing in real time.
Drawings
FIG. 1 is a schematic diagram of a stem cell culture status monitor;
FIG. 2 is a top view of a stem cell culture status monitor;
fig. 3 is a schematic structural diagram of an inflator in the stem cell culture state monitor.
The reference numerals in the schematic drawings illustrate: 1-a box body; 2-a leg; 3-box cover; 4, movable buckle; 5-a handle; 6-a first telescoping mechanism; 7-a circuit board; 8-a second telescoping mechanism; 9-electron microscope probe; 10-a control box; 11-an inflator; 12-a piston plate; 13-a metal tube; 14-a first one-way valve; 15-a second one-way valve; 16-a water conduit; 17-collection box.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention, and the following embodiments are combined to further describe the present invention.
Example 1
Referring to fig. 1-3, in the embodiment of the present invention, a stem cell culture state monitor comprises a box body 1 and a box cover 3, wherein a support leg 2 is installed at the bottom of the box body 1, a suction cup is installed at the lower end of the support leg 2, the device is firmly attached to a laboratory bench by the suction cup, so as to improve stability, the left end of the box body 1 is hinged to the left end of the box cover 3, a sealing ring is installed at the top edge of the box body 1, after the box cover 3 is closed, the box body 1 is kept in a sealing state, a movable buckle 4 is installed on the side wall of the box cover 3, the box cover 3 and the box body 1 can be tightly combined by the movable buckle 4, so as to ensure sealing performance, a handle 5 is arranged above the movable buckle 4, the handle 5 is installed on the box cover 3, a first telescopic mechanism 6 and a second telescopic mechanism 8 are installed inside the box cover 3, the, the first telescopic mechanism 6 and the second telescopic mechanism 8 are both electric hydraulic telescopic cylinders, and the lifting plate 7 is installed at the lower end of the first telescopic mechanism 6.
The last fixed surface of case lid 3 is connected with control box 10, the internal integration of control box 10 has the controller, the model of controller is KY02S, the last surface mounting of control box 10 has electron microscope, install a plurality of electrode sensor on the lifter plate 7, including dissolved oxygen electrode, the pH electrode, the carbon dioxide electrode, lifter plate 7 is through flexible wire and control box 10 electric connection, contain the stem cell culture solution in the box 1, the extension of the first telescopic machanism 6 of control, drive lifter plate 7 downstream, make electrode sensor submerge in the stem cell culture solution, dissolved oxygen content in the culture solution, the pH value, carbon dioxide content monitors in real time.
An inflator 11 is mounted on the upper surface of the case cover 3, the inflator 11 is communicated with the inside of the case cover 3, a second one-way valve 15 is mounted at the joint of the inflator 11 and the case cover 3, air in the inflator 11 can enter the case cover 3 through the second one-way valve 15, a piston plate 12 is arranged inside the inflator 11, the piston plate 12 is in sliding connection with the side wall of the case body 1, a metal pipe 13 penetrates through the piston plate 12, a handle is mounted at the upper end of the metal pipe 13, a first one-way valve 14 is mounted at the lower end of the metal pipe 13, outside air can enter the inflator 11 through the metal pipe 13, the metal pipe 13 is lifted upwards, the pressure in the inflator 11 is reduced, outside air enters the inflator 11 through the metal pipe 13, the metal pipe 13 is pressed downwards, the pressure in the inflator 11 is increased, air in the inflator 11 enters the case cover 3 through the second one-way valve 15, and, the left side of control box 10 is equipped with leading water pipe 16, leading water pipe 16 runs through case lid 3, the lower extreme of leading water pipe 16 stretches into in the box 1, leading water pipe 16 and case lid 3 sliding connection, the height-adjustable of leading water pipe 16, collecting box 17 is installed to the left side wall detachably of case lid 3, the upper end of leading water pipe 16 is passed through flexible hose and collecting box 17 intercommunication, when the culture solution sample is got to needs, insert the lower extreme of leading water pipe 16 in the culture solution, pull tubular metal resonator 13 from top to bottom, with the air injection this device in, under the effect of pressure, the culture solution flows into in collecting box 17 along leading water pipe 16, accomplish the sample operation.
The utility model discloses a theory of operation is: pouring stem cell culture solution into the box body 1, closing the box cover 3, controlling the extension of the first telescopic mechanism 6, driving the lifting plate 7 to move downwards, enabling the electrode sensor to be immersed in the stem cell culture solution, monitoring the dissolved oxygen content, the pH value and the carbon dioxide content in the culture solution in real time, when detecting that the oxygen content in the culture solution is insufficient, drawing the metal pipe 13 up and down, injecting air into the device, increasing the oxygen content in the culture solution, when a culture solution sample needs to be taken, inserting the lower end of the water diversion pipe 16 into the culture solution, drawing the metal pipe 13 up and down, injecting air into the device, and under the action of pressure, enabling the culture solution to flow into the collection box 17 along the water diversion pipe 16, and completing sampling operation.
Example 2
On the basis of embodiment 1, the lower end of the second telescoping mechanism 8 is provided with the electron microscope probe 9, the upper surface of the control box 10 is provided with the electron microscope, the electron microscope probe 9 is electrically connected with the electron microscope through a telescoping lead, the second telescoping mechanism 8 is controlled to extend, the electron microscope probe 9 is driven to move downwards, and the stem cells are observed by the electron microscope probe 9 and the control box 10.
It needs to explain very much, box 1, case lid 3, activity buckle 4 and handle 5 are prior art's application in this application, and the circuit board of liftable, electrode sensor, the electron microscope probe of liftable, inflater, leading water pipe, collecting box are the innovation point of this application, and it has effectively solved current stem cell culture apparatus can't monitor the process of cultivateing in real time, and the inconvenient problem of culture solution sample.
The present invention and its embodiments have been described above schematically, and the description is not limited thereto, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching of the present invention, without departing from the inventive spirit of the present invention, the person skilled in the art should also design the similar structural modes and embodiments without creativity to the technical solution, and all shall fall within the protection scope of the present invention.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. The utility model provides a stem cell culture state monitor, includes box (1) and case lid (3), a serial communication port, the inside of case lid (3) is equipped with first telescopic machanism (6), second telescopic machanism (8), lifter plate (7) are installed to the lower extreme of first telescopic machanism (6), install electrode sensor on lifter plate (7), the last surface mounting of case lid (3) has inflater (11), the inside of inflater (11) is equipped with piston board (12), it has tubular metal resonator (13) to run through on piston board (12), the last surface mounting of case lid (3) has control box (10), the left side of control box (10) is equipped with leading water pipe (16), collecting box (17) are installed to the left side wall detachably of case lid (3).
2. The stem cell culture state monitor according to claim 1, wherein an electron microscope probe (9) is mounted at the lower end of the second telescoping mechanism (8), an electron microscope is mounted on the upper surface of the control box (10), and the electron microscope probe (9) is electrically connected with the electron microscope through a telescoping lead.
3. The stem cell culture state monitor according to claim 1, wherein the bottom of the box body (1) is provided with a support leg (2).
4. The stem cell culture state monitor according to claim 3, wherein a suction cup is installed at the lower end of the support leg (2).
5. The stem cell culture state monitor according to any one of claims 1-4, wherein a movable buckle (4) is mounted on the side wall of the box cover (3), and a handle (5) is arranged above the movable buckle (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920082696.5U CN209989410U (en) | 2019-01-18 | 2019-01-18 | Stem cell culture state monitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920082696.5U CN209989410U (en) | 2019-01-18 | 2019-01-18 | Stem cell culture state monitor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209989410U true CN209989410U (en) | 2020-01-24 |
Family
ID=69287935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920082696.5U Expired - Fee Related CN209989410U (en) | 2019-01-18 | 2019-01-18 | Stem cell culture state monitor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209989410U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115612617A (en) * | 2022-12-06 | 2023-01-17 | 江苏中慧元通生物科技股份有限公司 | Biological vaccine multicellular reactor |
-
2019
- 2019-01-18 CN CN201920082696.5U patent/CN209989410U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115612617A (en) * | 2022-12-06 | 2023-01-17 | 江苏中慧元通生物科技股份有限公司 | Biological vaccine multicellular reactor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200124 |