CN115611974A

CN115611974A - Filtering and sterilizing equipment for preparing interferon purified protein

Info

Publication number: CN115611974A
Application number: CN202211302355.7A
Authority: CN
Inventors: 张顺荣; 蔡绘图; 陆凯磊; 刘惠
Original assignee: SHANGHAI HUAXIN HIGH BIOTECHNOLOGY Inc
Current assignee: SHANGHAI HUAXIN HIGH BIOTECHNOLOGY Inc
Priority date: 2022-10-24
Filing date: 2022-10-24
Publication date: 2023-01-17
Anticipated expiration: 2042-10-24
Also published as: CN115611974B

Abstract

The invention discloses a filtering and sterilizing device for preparing interferon purified protein, which relates to the technical field of interferon processing, and the device comprises a filtering and sterilizing frame, wherein a liquid inlet hopper is fixedly arranged at the top of the filtering and sterilizing frame, a filtering chamber is arranged below the liquid inlet hopper, the bottom of the liquid inlet hopper is communicated with the top of the filtering chamber, a drainage tube is fixedly arranged at the bottom of the liquid inlet hopper, a first drainage plate and a second drainage plate are fixedly arranged in the filtering chamber, a lifting hopper is slidably arranged between the first drainage plate and the second drainage plate, a dialysis membrane is fixedly arranged on the lifting hopper, a guide roller is rotatably arranged in the filtering chamber, and the guide roller can efficiently remove impurities on the surface of the dialysis membrane while vibrating and filtering the interferon solution by matching with the lifting hopper which is lifted repeatedly, so that the phenomenon that the surface of the dialysis membrane is blocked is avoided, and the filtering efficiency of the dialysis membrane is improved.

Description

Filtering and sterilizing equipment for preparing interferon purified protein

Technical Field

The invention relates to the technical field of interferon processing, in particular to a filtering and sterilizing device for preparing interferon purified protein.

Background

Interferons are glycoproteins with high species specificity, so that animal interferons are ineffective in humans, and have antiviral, cytostatic, immunomodulating and antitumor effects.

The preparation of purified protein by interferon requires the use of filter sterilization equipment, and the current filter sterilization equipment filters the interferon solution through a dialysis membrane and performs sterilization treatment through a sterilization lamp. The dialysis membrane in the existing filtration sterilization equipment is often easy to block, and the impurities on the surface of the dialysis membrane cannot be effectively removed by a conventional vibration structure, so that the dialysis membrane needs to be replaced or cleaned manually and periodically. And the sterilizing lamp in the filter sterilization equipment can perform effective sterilization treatment on all interferon solutions after irradiating for a period of time, and the sterilization efficiency is not high.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a filter sterilization device for preparing an interferon purified protein.

In order to realize the purpose, the invention provides the following technical scheme:

a filtration and sterilization device for preparing purified interferon protein comprises a filtration and sterilization frame, wherein a liquid inlet hopper is fixedly installed at the top of the filtration and sterilization frame, a filter chamber is arranged below the liquid inlet hopper, the bottom of the liquid inlet hopper is communicated with the top of the filter chamber, a drainage tube is fixedly installed at the bottom of the liquid inlet hopper, a first drainage plate and a second drainage plate are fixedly installed in the filter chamber, a lifting hopper is installed between the first drainage plate and the second drainage plate in a sliding manner, a dialysis membrane is fixedly installed on the lifting hopper, a guide roller is installed in the filter chamber in a rotating manner, a cleaning pump is fixedly installed on the outer wall of the filter chamber, a cleaning water tank is fixedly installed on one side of the cleaning pump, the water pumping end of the cleaning pump is communicated with the side wall of the cleaning water tank, the water pumping end of the cleaning pump is rotatably connected with an engagement pipe, one end of the engagement pipe, which is far away from the cleaning pump, is fixedly connected with the guide roller, a plurality of cleaning holes are formed in the surface of the guide roller, the guide roller is arranged right above the lifting hopper, and a telescopic pipe is fixedly installed at the bottom of the lifting hopper;

the filter chamber below is provided with except that the fungus room, flexible tub of bottom fixed mounting has the branch flow box, branch flow box below is provided with a plurality of first sterilamp, around being equipped with the winding pipe on the first sterilamp, winding pipe fixed connection divides flow box, the winding pipe is kept away from and is divided flow box one end fixed connection ring pipe, fixed mounting has a plurality of connecting pipes on the ring pipe, the equal fixed connection drain pipe of a plurality of connecting pipes, the interior rotation of degerming installs rotatory ring gear, fixed mounting has a plurality of second sterilamp on the rotatory ring gear.

Further, first drainage plate all is the slope setting with the second drainage plate, filter chamber inner wall bottom fixed mounting has lifting unit, lifting unit is used for the drive to go up and down to fight and go up and down.

Furthermore, a driving assembly is fixedly mounted on the outer wall of the filtering chamber and used for driving the guide roller to rotate.

Furthermore, a discharge hole is formed in one side of the filtering chamber, a drain pipe is fixedly mounted on the filtering and sterilizing frame and communicated with the discharge hole.

Furthermore, a plurality of first sterilamp are the equidistant annular distribution, and radian such as a plurality of connecting pipes fixed mounting is on the ring pipe.

Furthermore, one end of the liquid outlet pipe, which is far away from the connecting pipe, penetrates through the side wall of the degerming chamber.

Furthermore, radians such as a plurality of second sterilamp fixed mounting are on rotatory ring gear, degerming room inner chamber bottom fixed mounting has rotating assembly, rotating assembly is used for driving the rotatory gear, rotatory gear meshing connects rotatory ring gear.

Compared with the prior art, the invention has the following beneficial effects:

1. the rotary guide roller is arranged in the filter chamber and matched with the lifting hopper which is lifted repeatedly, so that the interferon solution can be efficiently removed from impurities on the surface of the dialysis membrane by the guide roller during vibration filtration, the blockage on the surface of the dialysis membrane is avoided, the filtration efficiency of the dialysis membrane is improved, after filtration and sterilization, the guide roller can spray water to correspondingly clean the dialysis membrane, the replacement and cleaning times of the dialysis membrane are reduced, the guide roller can not only play a role in improving the filtration efficiency of the dialysis membrane, but also can correspondingly clean the dialysis membrane;

2. set up a plurality of first sterilamp in the degerming room, and set up the winding pipe on first sterilamp, and set up a plurality of circulation pivoted second sterilamp in the degerming room, a plurality of winding pipes shunt to interferon solution, reduce the flow of interferon solution, and then improve the sterilizing effect and the efficiency of first sterilamp and second sterilamp to interferon solution, make interferon solution can obtain sterilization treatment at the in-process that flows, first sterilamp and second sterilamp guarantee to carry out the sterilization treatment at no dead angle to the interferon solution in the winding pipe.

Drawings

FIG. 1 is a schematic view showing the construction of a filtration sterilization apparatus for interferon purified protein production;

FIG. 2 is a view showing the inner structure of the filtering chamber of the present invention;

FIG. 3 is an internal structural view of a guide roller of the present invention;

FIG. 4 is an internal structure view of the lift bucket of the present invention;

figure 5 is a side view of the filtering chamber of the present invention;

FIG. 6 is a view showing the inner structure of the sterilization chamber according to the present invention;

FIG. 7 is an installation view of a first germicidal lamp in accordance with the invention;

FIG. 8 is a schematic view showing the structure of the ring tube of the present invention.

100. Filtering and sterilizing the rack; 101. a liquid inlet hopper; 102. a filtering chamber; 103. a drive assembly; 104. a guide roller; 1041. connecting the pipe; 1042. cleaning the holes; 105. a drainage tube; 106. a first drainage plate; 107. a second drainage plate; 108. a lifting bucket; 1081. a dialysis membrane; 109. a lifting assembly; 110. a telescopic pipe; 111. cleaning the pump; 112. cleaning a water tank; 113. a discharge hole; 114. a blow-off pipe; 200. a sterilization chamber; 201. a shunt box; 202. winding a pipe; 203. a first germicidal lamp; 204. an annular tube; 205. a connecting pipe; 206. a liquid outlet pipe; 207. a rotating assembly; 208. a rotating gear; 209. rotating the toothed ring; 210. a second germicidal lamp.

Detailed Description

Example 1

Referring to fig. 1 to 5, a filtration and sterilization device for preparing purified interferon protein, which includes a filtration and sterilization frame 100, a liquid inlet hopper 101 is fixedly installed on the top of the filtration and sterilization frame 100, a filter chamber 102 is disposed below the liquid inlet hopper 101, the bottom of the liquid inlet hopper 101 is communicated with the top of the filter chamber 102, a drainage tube 105 is fixedly installed on the bottom of the liquid inlet hopper 101, a first drainage plate 106 and a second drainage plate 107 are fixedly installed in the filter chamber 102, a lifting hopper 108 is slidably installed between the first drainage plate 106 and the second drainage plate 107, a dialysis membrane 1081 is fixedly installed on the lifting hopper 108, a guide roller 104 is rotatably installed in the filter chamber 102, a cleaning pump 111 is fixedly installed on the outer wall of the filter chamber 102, a cleaning water tank 112 is fixedly installed on one side of the cleaning pump 111, a water pumping end of the cleaning pump 111 is communicated with the side wall of the cleaning water tank 112, a water outlet end of the cleaning pump 111 is rotatably connected with an adapter tube 1041, one end of the adapter tube 1041, which is far away from the cleaning pump 111 is fixedly connected with the guide roller 104, a plurality of cleaning holes 1042 are disposed on the surface of the guide roller 104, the guide roller 104 is disposed right above the lifting hopper 108. First drainage plate 106 all is the slope setting with second drainage plate 107, and filter chamber 102 inner wall bottom fixed mounting has lifting unit 109, and lifting unit 109 can be cylinder, hydro-cylinder, and lifting unit 109 is used for driving lift fill 108 and goes up and down. The outer wall of filter chamber 102 is fixedly mounted with a driving assembly 103, wherein driving assembly 103 can be a motor, and driving assembly 103 is used for driving guide roller 104 to rotate. Discharge hole 113 is opened to filter chamber 102 one side, and fixed mounting has blow off pipe 114 on the filtration sterilization frame 100, and blow off pipe 114 communicates discharge hole 113. Set up rotatory guide roller 104 in filter chamber 102, the cooperation is the lift fill 108 that goes up and down repeatedly, make interferon solution guide roller 104 can get rid of the impurity high efficiency on dialysis membrane 1081 surface in the time of vibration filtration, avoid dialysis membrane 1081 surface the condition of blockking up to appear, and then improve dialysis membrane 1081's filtration efficiency, and after filtering the degerming, guide roller 104 can spray water and carry out corresponding washing to dialysis membrane 1081, reduce dialysis membrane 1081's change and washing number of times, guide roller 104 not only can play the effect that improves dialysis membrane 1081 filtration efficiency, can carry out corresponding washing to dialysis membrane 1081 simultaneously.

Example 2

Referring to fig. 6 to 8, on the basis of embodiment 1, a sterilization chamber 200 is disposed below a filter chamber 102, a diversion box 201 is fixedly mounted at the bottom of a telescopic tube 110, a plurality of first sterilamp 203 is disposed below the diversion box 201, a winding tube 202 is wound on the first sterilamp 203, the winding tube 202 is fixedly connected to the diversion box 201, one end of the winding tube 202, which is far away from the diversion box 201, is fixedly connected to a ring-shaped tube 204, a plurality of connecting tubes 205 are fixedly mounted on the ring-shaped tube 204, the connecting tubes 205 are all fixedly connected to a liquid outlet tube 206, a rotary toothed ring 209 is rotatably mounted in the sterilization chamber 200, and a plurality of second sterilamp 210 is fixedly mounted on the rotary toothed ring 209. The first sterilizing lamps 203 are distributed in an annular shape at equal intervals, and the connecting pipes 205 are fixedly arranged on the annular pipe 204 at equal radian. The end of the liquid outlet pipe 206 far away from the connecting pipe 205 penetrates through the side wall of the sterilization chamber 200. A plurality of second sterilizing lamps 210 are fixedly arranged on a rotating toothed ring 209 in an equal radian manner, a rotating component 207 is fixedly arranged at the bottom of the inner cavity of the sterilizing chamber 200, the rotating component 207 can be a motor or a rotary cylinder, the rotating component 207 is used for driving a rotating gear 208 to rotate, and the rotating gear 208 is meshed with the rotating toothed ring 209. Set up a plurality of first sterilamp 203 in degerming room 200, and set up winding pipe 202 on first sterilamp 203, and set up a plurality of circulative rotation's second sterilamp 210 in degerming room 200, a plurality of winding pipes 202 shunt interferon solution, reduce the flow of interferon solution, and then improve the sterilizing effects of first sterilamp 203 and second sterilamp 210 to interferon solution, first sterilamp 203 and second sterilamp 210 guarantee to carry out the sterilization at no dead angle to the interferon solution in winding pipe 202.

The working principle is as follows:

the method comprises the following steps: adding an interferon solution into a liquid inlet hopper 101, wherein the interferon solution flows to a first drainage plate 106 through a drainage pipe 105, the interferon solution is guided to a lifting hopper 108 by the first drainage plate 106, the lifting hopper 108 is repeatedly pushed and pulled by a lifting assembly 109, the lifting hopper 108 is repeatedly lifted and lowered between the first drainage plate 106 and a second drainage plate 107, meanwhile, a driving assembly 103 drives a guide roller 104 to rotate, the interferon solution passes through a dialysis membrane 1081 and flows into a telescopic pipe 110 in the lifting process of the lifting hopper 108, impurities are attached to the surface of the dialysis membrane 1081, and when the surface of the guide roller 104 is in contact with the surface of the dialysis membrane 1081 in the lifting process of the lifting hopper 108, the rotating guide roller 104 drives the impurities on the surface of the dialysis membrane 1081 to move towards the second drainage plate 107, and the impurities fall on the second drainage plate 107 and flow to a drainage pipe 114 through the second drainage plate 107;

step two: the interferon solution flows into the flow distribution box 201 through the telescopic tube 110, the interferon solution in the flow distribution box 201 flows into the winding tube 202, meanwhile, the rotating assembly 207 drives the rotating gear 208 to rotate, the rotating gear 208 drives the rotating toothed ring 209 to rotate, the rotating toothed ring 209 drives the plurality of second sterilizing lamps 210 to rotate, the first sterilizing lamp 203 is matched with the second sterilizing lamps 210 to sterilize the interferon solution in the winding tube 202, the sterilized interferon solution flows into the connecting tube 205, and the interferon solution flows into the liquid outlet tube 206 through the connecting tube 205;

step three: after the interferon solution is filtered and sterilized, the cleaning pump 111 pumps water in the cleaning water tank 112, and conveys the water into the guide roller 104 through the connecting pipe 1041, the water in the guide roller 104 is discharged through the cleaning hole 1042, meanwhile, the driving assembly 103 drives the guide roller 104 to rotate, the lifting assembly 109 drives the lifting bucket 108 to repeatedly lift, when the surface of the guide roller 104 is in contact with the surface of the dialysis membrane 1081 in the lifting process of the lifting bucket 108, the rotating guide roller 104 is matched with the water discharged from the cleaning hole 1042 to clean impurities remaining on the surface of the dialysis membrane 1081 onto the second flow guide plate 107, and part of the water flows into the telescopic pipe 110 through the dialysis membrane 1081 and sequentially cleans a subsequent shunt box 201, a winding pipe 202, a ring pipe 204, a connecting pipe 205 and a liquid outlet pipe 206.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the present template may occur to those of ordinary skill in the art without departing from the principles of the present invention.

Claims

1. The utility model provides a be used for interferon purified protein preparation with filtration degerming equipment, includes filtration degerming frame (100), its characterized in that, filtration degerming frame (100) top fixed mounting has into liquid fill (101), it is provided with filter chamber (102) to advance liquid fill (101) below, it has filter chamber (102) top to advance liquid fill (101) bottom intercommunication, it has drainage tube (105) to advance liquid fill (101) bottom fixed mounting, fixed mounting has first drainage plate (106), second drainage plate (107) in filter chamber (102), slidable mounting has between first drainage plate (106) and second drainage plate (107) and goes up and down fill (108), fixed mounting has dialysis membrane (1081) on going up and down fill (108), guide roller (104) is installed to filter chamber (102) internal rotation, filter chamber (102) outer wall fixed mounting has cleaning pump (111), cleaning pump (111) one side fixed mounting has cleaning water tank (112), cleaning pump (111) end intercommunication cleaning water tank (112) lateral wall draws water, cleaning pump (111) outlet end rotation connection takeover (1041), it connects takeover (1041) to join in, cleaning pump (111) one end fixed mounting has kept away from cleaning pump (104) surface connection hole (1042) and has just seted up and has guide roller (1042) guide roller (104), an extension tube (110) is fixedly arranged at the bottom of the lifting bucket (108);

filter chamber (102) below is provided with except that fungus room (200), flexible pipe (110) bottom fixed mounting has branch flow box (201), branch flow box (201) below is provided with a plurality of first sterilamp (203), around being equipped with winding pipe (202) on first sterilamp (203), winding pipe (202) fixed connection branch flow box (201), branch flow box (201) one end fixed connection ring venturi tube (204) are kept away from in winding pipe (202), fixed mounting has a plurality of connecting pipes (205) on ring duct (204), the equal fixed connection drain pipe (206) of a plurality of connecting pipes (205), remove fungus room (200) internal rotation and install rotatory ring gear (209), fixed mounting has a plurality of second sterilamp (210) on rotatory ring gear (209).

2. The filtration and sterilization apparatus for interferon purified protein preparation according to claim 1, wherein the first drainage plate (106) and the second drainage plate (107) are both disposed in an inclined manner, and a lifting assembly (109) is fixedly installed at the bottom of the inner wall of the filtration chamber (102), wherein the lifting assembly (109) is used for driving the lifting bucket (108) to lift.

3. The filtration sterilization apparatus for interferon purified protein preparation according to claim 2, wherein a driving assembly (103) is fixedly installed on the outer wall of the filtration chamber (102), and the driving assembly (103) is used for driving the guide roller (104) to rotate.

4. The filtration and sterilization equipment for preparing purified interferon protein according to claim 3, wherein a discharge hole (113) is opened at one side of the filtration chamber (102), a sewage pipe (114) is fixedly installed on the filtration and sterilization rack (100), and the sewage pipe (114) is communicated with the discharge hole (113).

5. The filter sterilization apparatus for interferon purified protein preparation according to claim 4, wherein the plurality of first sterilization lamps (203) are distributed in an equally spaced ring shape, and the plurality of connection tubes (205) are fixedly installed on the ring-shaped tube (204) in an equally arc shape.

6. The filter sterilization apparatus for interferon purified protein preparation according to claim 1, wherein the end of the outlet tube (206) far from the connection tube (205) penetrates the side wall of the sterilization chamber (200).

7. The filtration and sterilization apparatus for preparing purified interferon protein according to claim 6, wherein a plurality of second sterilization lamps (210) are fixedly installed on a rotating toothed ring (209) in an equal arc, a rotating assembly (207) is fixedly installed at the bottom of the inner cavity of the sterilization chamber (200), the rotating assembly (207) is used for driving a rotating gear (208) to rotate, and the rotating gear (208) is engaged with the rotating toothed ring (209).