CN115572662A - Swab cleaning device in biological reaction card box and cleaning method thereof - Google Patents
Swab cleaning device in biological reaction card box and cleaning method thereof Download PDFInfo
- Publication number
- CN115572662A CN115572662A CN202211401936.6A CN202211401936A CN115572662A CN 115572662 A CN115572662 A CN 115572662A CN 202211401936 A CN202211401936 A CN 202211401936A CN 115572662 A CN115572662 A CN 115572662A
- Authority
- CN
- China
- Prior art keywords
- swab
- cavity
- hole
- swab head
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004140 cleaning Methods 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 16
- 238000003860 storage Methods 0.000 claims abstract description 66
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 238000005406 washing Methods 0.000 claims abstract description 19
- 238000001514 detection method Methods 0.000 claims description 28
- 239000000126 substance Substances 0.000 claims description 19
- 239000000835 fiber Substances 0.000 claims description 9
- 239000002657 fibrous material Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000001746 injection moulding Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000004033 plastic Substances 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 46
- 239000000243 solution Substances 0.000 description 30
- 238000012123 point-of-care testing Methods 0.000 description 25
- 238000005070 sampling Methods 0.000 description 21
- 238000012360 testing method Methods 0.000 description 21
- 239000000047 product Substances 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 8
- 239000012530 fluid Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000003745 diagnosis Methods 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 208000035473 Communicable disease Diseases 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 206010068516 Encapsulation reaction Diseases 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Abstract
The invention provides a swab cleaning device in a biological reaction card box and a cleaning method thereof, wherein the swab cleaning device comprises a swab head receiving cavity, a swab head cleaning cavity and a swab head cleaning cavity, wherein the swab head receiving cavity is used for receiving a swab head after a sample is collected; the storage cavity is used for storing clean washing liquid and/or washing liquid carrying a sample; a mixing chamber for storing clean washing liquid and/or repeatedly infiltrating the swab head with clean washing liquid to elute the sample. The device has simple processing technology, meets the requirement of plastic material injection molding technology, and is suitable for mass production; the standardized treatment of the sample can be achieved, and the sample pretreatment result can reach the same level as that of the sample pretreatment result of the laboratory swab cleaning.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a swab cleaning device in a biological reaction card box and a cleaning method thereof.
Background
The advanced transportation system provides great convenience for the travel of human beings and various production and living services. However, high frequency human activities and population migration also create opportunities for the wide spread of various infectious diseases. In the traditional infectious disease detection method, samples of a detected object are collected and then are collectively sent to a central laboratory or a designated medical fixed-point institution for detection, and a detection report can be issued only within days to a week. When dealing with the epidemic situation of a large-scale high-risk infectious disease, the traditional laboratory detection method often cannot provide the detection data of the first time for the prevention and control of the epidemic situation, so that the development of the instant fixed-point rapid detection technology and products is an indispensable task in the current society.
In recent years, more and more in vitro diagnostic suppliers and scientific research units develop Point-of-care-testing (POCT) products suitable for rapid on-site testing. The appearance of POCT products changes the traditional infectious disease detection process and method, so that suspected cases can be sampled and detected on site, detection results can be obtained in a very short time or even in real time, and the traditional detection time is greatly shortened. In addition, POCT detection products can be independent of a traditional central laboratory, the flow required by reaction is designed in a disposable test box, the POCT detection products can work in cooperation with a miniaturized portable instrument, professional trained personnel or related personnel are not needed to operate after being simply trained, and popularization of a graded medical system is greatly accelerated.
Although POCT in vitro diagnostic products have many advantages, they also present some problems during use. For example, the most common sampling methods in the detection of infectious diseases in the respiratory system are simple and easy-to-operate methods such as throat swab, oronasal swab, nasopharyngeal swab and the like, wherein an operator holds the swab and inserts the swab into the respiratory tract of a patient to be detected, scrapes mucus or epithelial cells, then puts the swab head into a preservative fluid or cell lysate, repeatedly washes, stands, extracts supernatant, and tests on a machine. In most cases, the quality of the sample collection and the pre-processing on the machine of the original sample often directly affects the interpretation of the detection result. Most of the actual operators for in vitro diagnosis of POCT products are not medical staff trained professionally, and the collected samples cannot be subjected to standardized on-machine pretreatment by virtue of large-scale laboratory automation equipment in an instant diagnosis environment, so that the quality of the samples to be detected on the on-machine equipment is often very different. The method directly influences the accuracy of interpretation results of POCT products in vitro diagnosis, and is also one of the main reasons that the detection accuracy of the POCT products is questioned by all parties. Therefore, when the POCT apparatus and the test box for in vitro diagnosis are designed, the links of cleaning the swab head, extracting supernatant and the like are included, so that the manual operation process is reduced, more importantly, the pretreatment of a part of samples on the computer can be completed by standardizing the apparatus and the test box, and the POCT apparatus and the test box are very important for improving the accuracy of interpretation of test results. Unfortunately, most in vitro diagnostic POCT-like products currently on the market do not perform this function, based on considerations of cost and complexity of design development.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
In view of the shortcomings of the prior art, it is an object of the present invention to provide a swab washing device in a biological reaction cartridge and a washing method thereof.
According to the present invention, there is provided a swab washing device in a bioreaction cartridge, comprising a swab housing box including a swab head receiving chamber, a storage chamber and a mixing chamber, wherein:
the swab head receiving cavity and the storage cavity are respectively communicated with the mixing cavity;
the swab head receiving cavity is used for receiving a swab head which collects a sample;
the storage cavity is used for storing clean cleaning liquid and/or cleaning liquid carrying a sample;
the mixing chamber is used for storing clean cleaning liquid and/or repeatedly infiltrating the swab head with the clean cleaning liquid to elute the sample.
Preferably, the swab head receiving chamber and the storage chamber are both sealed chambers, and the mixing chamber is arranged at the bottom of the swab head receiving chamber and the storage chamber;
the top of the swab head receiving cavity is provided with a plug opening and a first through hole for controlling air pressure, and the bottom of the swab head receiving cavity is provided with a first channel communicated with the mixing cavity;
the top of the storage cavity is provided with a second through hole for controlling air pressure, and the bottom of the storage cavity is provided with a second channel for communicating the mixing cavity and a third channel for connecting other equipment;
the mixing cavity mainly comprises a hollow cylinder and a piston capable of reciprocating in the hollow cylinder, and the hollow cylinder on the top of the piston is communicated with the swab head receiving cavity and the storage cavity through a first passage and a second passage respectively.
Preferably, the swab head receiving cavity, the storage cavity and the mixing cavity are all sealed cavities, the swab head receiving cavity is obliquely inserted on the side wall of the mixing cavity, and the mixing cavity is communicated with the storage cavity through an oblique through hole which is inclined downwards;
the top of the swab head receiving cavity is provided with a plug opening;
the top parts of the storage cavity and the mixing cavity are respectively provided with a second through hole and a third through hole for controlling air pressure, and the bottom part of the storage cavity is provided with a third channel which can be connected with other equipment;
establish in the hybrid chamber along cavity reciprocating motion's piston, cavity and oblique through-hole mutually support and form: when the piston is arranged between the inclined through hole and the cavity, the piston and the cavity enclose a chamber for containing cleaning liquid, and when the piston moves to the position below the inclined through hole, the cleaning liquid flows to the storage cavity along the inclined through hole.
Preferably, the diameter of the first channel is smaller than the diameter of the swab shaft.
Preferably, the size of the opening is greater than the size of the swab head fibrous substance.
Preferably, the plugged openings comprise a perforated opening and a branched opening in communication, wherein:
the size of the macroporous open-cell fibrous substance is larger than that of the swab head;
the size of the branch opening is substantially the same as the size of the swab rod at the groove.
Preferably, the opening is provided with a sealing plug having a protrusion substantially conforming in shape to the opening.
According to the invention, the cleaning method based on the swab cleaning device in the biological reaction cartridge comprises the following steps:
step A1: pinching off the end of the swab from which the sample is collected into the swab head receiving cavity, closing the opening of the swab head receiving cavity;
step A2: closing the first through hole and the third channel, opening the second through hole, moving the piston downwards, and naturally flowing the cleaning liquid in the storage cavity into the mixing cavity from the second channel;
step A3: closing the second through hole, opening the first through hole, moving the piston upwards, spraying the cleaning solution in the mixing cavity into the swab head receiving cavity through the first channel to soak the fiber substance containing the sample, and moving the piston downwards after the cleaning solution in the mixing cavity is completely sprayed into the swab head receiving cavity through the first channel, so that the cleaning solution in the swab head receiving cavity flows back into the mixing cavity;
step A4: repeating the step A3 for a certain number of times;
step A5: and closing the first through hole and opening the second through hole, moving the piston upwards, and enabling the cleaning liquid in the mixing cavity to flow into the storage cavity through the second channel to finish the collection of the cleaning liquid.
According to the invention, the cleaning method based on the swab cleaning device in the biological reaction card box comprises the following steps:
step B1: pinching off the end of the swab from which the sample is collected, the swab head passing through the swab head receiving chamber and being immersed in the washing liquid in the mixing chamber, closing the opening of the swab head receiving chamber;
and step B2: closing the second through hole, the third channel and opening the first through hole, and reciprocating the piston between the swab head and the inclined through hole for a certain number of times;
and step B3: opening the second through hole, moving the piston downwards until the inclined through hole is exposed, and naturally flowing the cleaning solution into the storage cavity along the inclined through hole to finish the collection of the sample cleaning solution;
and step B4: the piston moves upwards and is blocked at the inclined through hole.
Preferably, the third channel is externally connected with the detection device, and the collected cleaning liquid flows into the externally connected device through the third channel.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention has simple structure and is easy to realize the sample pretreatment function by combining the design of the in-vitro diagnosis POCT detection box.
2. The invention has simple processing technology, meets the technical requirement of plastic material injection molding and is suitable for mass production.
3. The invention has good treatment effect and can reach the same level with the sample pretreatment result of the laboratory swab cleaning.
4. The invention reduces the manual process by the matching of the swab head receiving cavity, the storage cavity and the mixing cavity, and greatly improves the accuracy of detection and judgment by a machine.
5. The invention skillfully utilizes the up-and-down movement of the piston and the air pressure control hole to control the air pressure in different cavities, thereby being capable of selectively limiting different flowing directions of the fluid.
6. In the invention, after the initial state and the cleaning process of the swab head are finished, the separation between the sample receiving cavity and the cleaning solution storage cavity is realized by utilizing the piston in the mixing device, so that a complicated valve system and a complicated valve mechanism are omitted.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic view of a sampling swab.
FIG. 3 is a schematic view of the cover of the swab washing device in the bioreaction cartridge.
FIG. 4 is a schematic view of a swab entering a cleaning device.
Fig. 5 is a perspective structure diagram of the cleaning device.
FIG. 6 is a schematic view of the cleaning step 1 in example 1.
FIG. 7 is a schematic view of the cleaning step 2 in example 1.
FIG. 8 is a schematic view of the cleaning step 3 in example 1.
FIG. 9 is a schematic view of the cleaning step 5 in example 1.
FIG. 10 is a schematic view of the cleaning step 1 in example 2.
FIG. 11 is a schematic view of the cleaning step 2 in example 2.
FIG. 12 is a schematic view of the cleaning step 3 in example 2.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
As shown in fig. 1 to 12, the invention provides a sampling swab which comprises a swab rod and sampling fiber substances wrapped at one end of the swab rod, wherein a reducing part for reducing the strength of the rod body is arranged on the swab rod which is away from the sampling fiber substances by a preset distance. Wherein the reducing part is a groove or a notch.
The invention also provides a swab cleaning device in the biological reaction card box, which comprises a swab accommodating box, wherein the swab accommodating box mainly comprises: a swab head receiving cavity for receiving a swab head after collection of a sample; the storage cavity is used for storing clean cleaning liquid and/or cleaning liquid carrying a sample; a mixing chamber for storing clean washing liquid and/or repeatedly infiltrating the swab head with clean washing liquid to elute the sample.
In the technical scheme, the swab head receiving cavity and the storage cavity are both sealed cavities, and the mixing cavity is arranged at the bottoms of the swab head receiving cavity and the storage cavity; the top of the swab head receiving cavity is provided with a plug opening and a first through hole for controlling air pressure, and the bottom of the swab head receiving cavity is provided with a first channel communicated with the mixing cavity; the top of the storage cavity is provided with a second through hole for controlling air pressure, and the bottom of the storage cavity is provided with a second channel for communicating the mixing cavity and a third channel for connecting other equipment; the mixing cavity mainly comprises a hollow cylinder and a piston capable of reciprocating in the hollow cylinder, and the hollow cylinder on the top of the piston is communicated with the swab head receiving cavity and the storage cavity through a first passage and a second passage respectively.
The invention adopts the method for cleaning the swab in the biological reaction card box, which comprises the following steps:
s1, pinching off the end part of the swab with the collected sample, putting the end part of the swab into a swab head receiving cavity, and closing an opening of the swab head receiving cavity; specifically, a swab from which a sample is collected is inserted into the swab head receiving cavity and pinched off using the branch opening and the reduced diameter portion of the swab rod.
S2, closing the first through hole and the third channel, opening the second through hole, moving the piston downwards, and naturally flowing the cleaning liquid in the storage cavity into the mixing cavity from the second channel;
s3, closing the second through hole, opening the first through hole, moving the piston upwards, spraying the cleaning liquid in the mixing cavity into the swab head receiving cavity through the first channel, infiltrating the fiber substance containing the sample, moving the piston downwards after the cleaning liquid in the mixing cavity is completely sprayed into the swab head receiving cavity through the first channel, and enabling the cleaning liquid in the swab head receiving cavity to flow back into the mixing cavity;
s4, repeating the step S3 for a certain number of times;
and S5, closing the first through hole, opening the second through hole, moving the piston upwards, and enabling the cleaning liquid in the mixing cavity to flow into the storage cavity through the second channel to finish the collection of the cleaning liquid.
In more detail, the swab head receiving cavity, the storage cavity and the mixing cavity are all sealed cavities, the swab head receiving cavity is obliquely inserted on the side wall of the mixing cavity, and the mixing cavity is communicated with the storage cavity through an oblique through hole which is inclined downwards; the top of the swab head receiving cavity is provided with a plug opening; the top parts of the storage cavity and the mixing cavity are respectively provided with a second through hole and a third through hole for controlling air pressure, and the bottom part of the storage cavity is provided with a third channel which can be connected with other equipment; establish in the hybrid chamber along cavity reciprocating motion's piston, cavity and oblique through-hole mutually support and form: when the piston is arranged between the inclined through hole and the cavity, the piston and the cavity enclose a cavity for containing cleaning liquid, and when the piston moves to the lower part of the inclined through hole, the cleaning liquid flows to the storage cavity along the inclined through hole.
The first channel has a diameter less than the diameter of the swab shaft. The size of the opening is larger than that of the swab head fiber substance; the openings have a large-pore opening of a size larger than the fibrous substance of the swab head and a branch opening of a size substantially corresponding to the size of the swab rod at the groove. The opening is provided with a sealing plug which is connected or not connected with the opening, and the sealing plug is provided with a bulge with a shape basically consistent with the opening. The second channel or the inclined through hole is internally provided with a filter element. And the third channel is blocked.
The invention also provides a swab cleaning method in the biological reaction card box, which comprises the following steps: s1, cutting off the end part of a swab which collects a sample, enabling a swab head to penetrate through a swab head receiving cavity and be immersed in cleaning liquid in a mixing cavity, and closing an opening of the swab head receiving cavity; s2, closing the second through hole, opening the third channel and opening the third through hole, wherein the piston moves between the swab head and the inclined through hole for a certain number of times in a reciprocating mode; s3, opening the second through hole, moving the piston downwards until the inclined through hole is exposed, and naturally flowing the cleaning liquid into the storage cavity along the inclined through hole to finish the collection of the sample cleaning liquid; and S4, moving the piston upwards and blocking the inclined through hole. In the above technical scheme, the third channel is externally connected with the detection device, and the collected cleaning liquid flows into the external device through the third channel.
Example 1
The present invention provides a swab washing device 20 in a bioreactor cartridge, as shown in FIG. 1, comprising a main structure 23 and a mixing mechanism 24. The main structure 23 has a cover 22 and a plug 21 at the top. The plug 21 is connected to the main structure 23 by a flexible connection 211, but the plug 21 may be added separately if necessary without being connected to the main structure 23. Below the body structure 23 there is a mixing device 24.
Sampling swab 10 as shown in fig. 2, a sampling swab 10 commonly used in clinical or biological laboratories comprises a swab shaft 11 and a swab head 13. The forward end of the swab head 13 typically contains a polypropylene or cotton fiber substance 131 for collecting a patient sample. The operator typically holds the swab shaft 11, advances the swab head 13 into the patient's sampling site, and manually rotates or scrapes the patient's sampling site to adhere the sample to be collected to the fibrous material 131. The swab 10 after collection of the sample is returned to the central laboratory in a sample tube containing a sample preservative fluid, or is added directly to a POCT test apparatus (e.g., as described herein) for testing. In general, the collected sample is required to be sent to a laboratory for detection as soon as possible due to external pollution, nucleic acid degradation or sample deterioration. Too long a transport time often has a great influence on the accuracy of the detection result. The appearance of POCT in-vitro diagnosis products greatly reduces the uncertainty caused by the sample transportation link in the traditional detection process, and realizes the in-situ real-time sampling and in-situ real-time detection. A recess 12 is provided between the shaft 11 and the head 13 of the sampling swab. The swab 10 can be easily broken by applying an external force to the recess 12, so that the stem 11 and the head 13 of the swab are separated for further processing.
As shown in FIGS. 3 and 4, the swab washing device 20 of the bioreactor cartridge is covered with a cover 22, which is used to close the cavity inside the main structure 23 and optionally form a semi-open or completely closed space. There are 3 openings in the cover 22, wherein the holes 221 and 222 are air pressure control holes, connecting two chambers in the main structure 23. When the POCT test cartridge is placed inside the instrument, the open/close state of the air pressure control holes 221 and 222 can be controlled by a pressure connection member in the instrument, thereby adjusting the air pressure inside the chamber. The hole 223 is a swab insertion hole that is sized larger than the diameter of the swab head 13 containing the fibrous substance 131 so that an operator can easily insert the swab into the POCT test cartridge. The swab insertion hole 223 has a large hole 223a and a branched opening 223b.
The large hole 223a is used for receiving the sampling swab head 13 into the main structure 23, as shown in fig. 4, after the operator inserts the sampling swab 10 into the main structure 23, the sampling swab rod 11 is pulled slightly, so that the groove structure 12 on the sampling swab 10 enters the branch opening 223b, and then the swab rod 11 is bent slightly, so that the sampling swab is broken at the groove 12, and the swab head 13 is left in the POCT test box.
Referring to fig. 6, the plug 21 has a raised structure 212 thereon and mates with the swab insertion aperture 223, such that the swab insertion aperture 223 is completely closed when the plug 21 is closed. After the head 13 of the sampling swab 10 enters the body structure 23, the swab shaft 11 is discarded and the aperture plug 21 is closed. The swab insertion hole 223 in the cover 22 is now completely closed.
Fig. 5 is a transparent view of the swab head 13 inside the POCT test cassette after it has been inserted into the POCT test cassette and the plug has been closed. The main body structure 23 contains a cleaning liquid storage chamber 231 and a swab head receiving chamber 232, and a pipe 231a and a pipe 232a are provided below the cleaning liquid storage chamber 231 and the swab head receiving chamber 232, respectively, to communicate with the mixing device 24, respectively. Two air pressure control holes 221 and 222 on the cap 22 communicate with the cleaning liquid storage chamber 231 and the swab head receiving chamber 232, respectively, for adjusting and controlling the air pressure inside the two chambers. The mixing device 24 has a movable piston 241 inside, and the piston 241 can move up and down inside the mixing device 24 by an external force. In the initial state, the piston 241 is at the uppermost position inside the mixing device 24, and the upper surface of the piston 241 is closely attached to and closes the outlets of the passages 231a and 232 a. The air pressure control holes 221 and 222 are also in a closed state.
FIG. 6 is a schematic view showing the operation principle of cleaning the inside of the test cartridge using the swab cleaning apparatus in the biological reaction cartridge. The cleaning solution storage chamber 231 stores a cleaning solution 30 for cleaning the sampling swab in advance. The cleaning solution storage chamber 231 also has a connecting line 231c for connecting the swab cleaning device to other parts of the POCT test cartridge interior.
In the initial state, the connection pipe 231c is closed, and thus the cleaning liquid 30 is stored in the cleaning liquid storage chamber 231. When the sampling swab head 13 enters the swab head receiving cavity 232 and completely closes the swab insertion aperture 223 with the aperture plug 21. At this time, the air pressure control hole 221 is opened and the piston 241 is pulled to move downward, and an air pressure difference is formed between the inside of the cleaning solution storage chamber 231 and the upper space of the mixing device after the piston moves downward, so that the cleaning solution 30 enters the mixing device 24 through the passage 231a (fig. 7). After the cleaning liquid 30 completely enters the mixing device 24, the air pressure control hole 221 is closed and the air pressure control hole 222 is opened, and the piston 241 is pushed upwards by the driving device inside the apparatus. At this time, the cleaning liquid enters the swab head receiving chamber 232 through the passage 232 a. Since the size of the passage 232a is much smaller than the characteristic dimensions of the mixing device 24 and the swab head receiving chamber 232, the cleaning liquid 30 can be caused to form a high velocity fluid jet inside the swab head receiving chamber 232 (figure 8) and a strong scouring action on the fibrous substance 131 of the swab head 13 by designing the diameter and angle of the passage 232a under the action of the upward pressure of the piston. The length of the swab head 13 and the structural dimensions inside the swab head receiving chamber 232 are carefully designed so that the fibrous substance 131 of the swab head 13, after the swab 10 has been broken, can naturally fall to the bottom of the swab head receiving chamber 232, and corresponds to the location of the channel 232 a. Thus, the fiber material 131 can be effectively cleaned by the fluid beam formed by the reciprocating motion of the piston.
Referring to fig. 7 and 8, the piston 241 is repeatedly driven to move up and down for a plurality of times in the mixing device 24, and the swab head 13 can be repeatedly washed by the high-speed fluid beam for a plurality of times until all or part of the detected sample adhered to the fiber substance at the front end of the swab head 13 enters the cleaning liquid and the sample amount required by detection is achieved according to the test requirement. The piston 241 is then pushed by external drive to the lower part of the mixing device 24, so that the washing liquid 31 containing the sample to be tested completely enters the interior of the mixing device 24.
Referring to fig. 9, when the air pressure control hole 222 is closed and the air pressure control hole 221 is opened, the piston 241 is driven to move upward, and the cleaning solution 31 containing the sample to be measured can be pushed into the cleaning solution storage chamber 231. Between the channel 231a and the cleaning solution storage chamber 231, there is a filter element 231b, which can filter out the impurities and large particles in the cleaning solution 31 containing the sample to be tested, and ensure the smooth implementation of the next detection operation in the POCT detection box (fig. 9). After the cleaning solution 31 containing the sample to be tested is pushed into the cleaning solution storage chamber 231, the piston 241 is driven to the uppermost end in the mixing device 24, and the air pressure control hole 222 is closed, so that the channels 231a and 232a are completely closed, and the next reaction is ensured to be carried out in a closed space without being influenced by external substances and potential pollution. When the cleaning solution 31 containing the sample to be tested needs to be transported to other parts of the POCT test cassette, the air pressure control hole and the connection pipeline 231c are opened, so that the transfer of the sample cleaning solution can be completed.
The invention utilizes the up-and-down movement of the piston and the air pressure control hole to control the air pressure in different cavities, thereby being capable of selectively limiting different flowing directions of the fluid. And the separation between the sample-receiving chamber and the cleaning solution storage chamber is achieved by means of a piston in the mixing device after the initial state and the swab head cleaning process are finished, thereby eliminating complex valve circuitry and mechanisms, which is one of the most innovative points of the present invention.
The swab cleaning device of the present invention is also applicable to liquid samples. In some detection procedures, samples from different sources are pretreated, mixed, centrifuged or stood in a laboratory, and then supernatant is extracted and used as an experimental sample to be tested on a machine. For such samples, the operator uses a disposable pipette to add the sample into the POCT test cassette through the swab head receiving cavity, and then closes the hole plug 21. At this time, similar to the above-mentioned working process, the opening and closing of the piston 241 and the air pressure control holes 221 and 222 are controlled, so that the sample and the cleaning solution (or other reaction solution such as lysis solution and the like and the encapsulation reaction solution) are sufficiently and uniformly mixed in the mixing device, and then transferred to the cleaning solution storage chamber 231.
Example 2
Referring to fig. 7, another embodiment of the present invention is shown. The swab washing device 40 in the bioreactor cartridge, which is basically similar in principle to the embodiment 1 described above, also includes a swab head receiving chamber 43, a mixing device 45 and a storage chamber 44, but is slightly different from the embodiment described above. The swab head receiving chamber 43 is angled with respect to the mixing device 45 and communicates with the interior of the mixing device 45. The mixing device 45 communicates with the reservoir chamber 44 via a passage 44a, and the passage 44a is inclined in the same direction as the swab head receiving chamber 43, so that it can be manufactured by a single drawing operation using a plastic injection moulding process. The mixing device 45 may be in the same plane as the upper portion of the reservoir 44 or in a different plane. When in different planes, the mixing device 45 and the storage chamber 44 have covers 42a and 42b, respectively. Each closure has a gas pressure control port 421 and 422, which communicate with the mixing device 45 and the storage chamber 44, respectively. The cleaning liquid 50 for swab head fiber substance cleaning is held in advance inside the mixing device 45, and, in an initial state, the piston 451 inside the mixing device 45 closes the passage 44a, so that the cleaning liquid 50 cannot enter the storage chamber 44. When the operator places a sampling swab inside the swab head receiving chamber 43, the swab 10 is broken off so that the swab head 13 falls inside the swab head receiving chamber 43, and the sample inlet to the sampling swab head receiving chamber is then closed with the aperture plug 41. By careful design, after the swab 10 is broken and the aperture plug 41 is closed, the swab head fibrous material 131 is just inside the mixing device 45 and in contact with the pre-stored cleaning solution 50. The swab head receiving chamber is sized to just receive the swab head 13 therein, so that the swab head 13 cannot rotate or move automatically inside the swab head receiving chamber 43 after the aperture plug 41 is closed. In this way, the fibrous mass of the swab head is always inside the mixing device and cannot move freely. At this time, the external driving mechanism drives the piston 451 to reciprocate up and down through the lower portion 45b of the mixing device, thereby realizing a cleaning action for the swab head fibrous substance 451. To ensure that the cleaning liquid is always located inside the mixing device 45, the upper surface of the piston 451 is always not lower than the opening position of the passage 44a during the up-and-down reciprocating movement of the piston. By controlling the external piston drive, the above-mentioned functions can be achieved relatively easily. The sample to be detected to which the fiber substance at the front end of the swab head 13 is adhered completely or partially enters the cleaning solution, and the sample amount required for detection is achieved according to the test requirement, the piston 451 descends under the driving of external driving, and the upper surface of the piston 451 is flush with the lowest end of the opening of the channel 44 a. At this time, the sample-containing washing liquid 51 in the upper portion 45a of the mixing device 45 enters the storage chamber 44 by its own weight. A pressure differential may also be applied between the mixing device 45 and the reservoir 44 by the POCT instrument to allow the sample-containing wash solution 51 to more easily enter the reservoir 44. After the washing liquid 51 containing the sample enters the reservoir chamber 44, the piston 451 moves upward to close the passage 44a, so that the next reaction proceeds in the closed space and isolated from the swab head receiving chamber. When the cleaning solution 31 containing the sample to be tested needs to be transported to other parts of the POCT test cell, the air pressure control hole and the connection pipeline 44b are opened, so that the transfer of the sample cleaning solution can be completed.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. A swab cleaning apparatus in a biological reaction cartridge, comprising a swab containment cartridge comprising a swab head receiving chamber, a storage chamber, and a mixing chamber, wherein:
the swab head receiving cavity and the storage cavity are respectively communicated with the mixing cavity;
the swab head receiving cavity is used for receiving a swab head which collects a sample;
the storage cavity is used for storing clean cleaning solution and/or cleaning solution carrying a sample;
the mixing chamber is used for storing clean cleaning liquid and/or repeatedly infiltrating the swab head with the clean cleaning liquid to elute the sample.
2. The swab cleaning device in a bioreaction cartridge of claim 1, wherein the swab head receiving chamber and the storage chamber are sealed chambers, and the mixing chamber is disposed at the bottom of the swab head receiving chamber and the storage chamber;
the top of the swab head receiving cavity is provided with a plug opening and a first through hole for controlling air pressure, and the bottom of the swab head receiving cavity is provided with a first channel communicated with the mixing cavity;
the top of the storage cavity is provided with a second through hole for controlling air pressure, and the bottom of the storage cavity is provided with a second channel for communicating the mixing cavity and a third channel for connecting other equipment;
the mixing cavity mainly comprises a hollow cylinder and a piston capable of reciprocating in the hollow cylinder, and the hollow cylinder on the top of the piston is communicated with the swab head receiving cavity and the storage cavity through a first passage and a second passage respectively.
3. The swab cleaning device in a bioreaction cartridge of claim 1, wherein the swab head receiving chamber, the storage chamber and the mixing chamber are sealed chambers, the swab head receiving chamber is obliquely inserted on the side wall of the mixing chamber, and the mixing chamber is communicated with the storage chamber through an oblique through hole which is inclined downwards;
the top of the swab head receiving cavity is provided with a plug opening;
the top parts of the storage cavity and the mixing cavity are respectively provided with a second through hole and a third through hole for controlling air pressure, and the bottom part of the storage cavity is provided with a third channel which can be connected with other equipment;
establish in the hybrid chamber along cavity reciprocating motion's piston, cavity and oblique through-hole mutually support and form: when the piston is arranged between the inclined through hole and the cavity, the piston and the cavity enclose a chamber for containing cleaning liquid, and when the piston moves to the position below the inclined through hole, the cleaning liquid flows to the storage cavity along the inclined through hole.
4. The swab cleaning device of claim 2, wherein the first channel has a diameter less than a diameter of the swab shaft.
5. The swab cleaning device in a bioreaction cartridge of claim 2 or 3, wherein the opening is larger in size than the swab head fibrous material.
6. The swab washing device in a bioreaction cartridge of claim 5, wherein the stoppered opening comprises a perforated opening and a branched opening in communication, wherein:
the size of the macroporous open-cell fibrous substance is larger than that of the swab head;
the size of the branch opening is substantially the same as the size of the swab rod at the groove.
7. The swab cleaning apparatus in a bioreaction cartridge of claim 5, wherein the opening is provided with a sealing plug having a protrusion substantially conforming to the shape of the opening.
8. A method for cleaning a swab in a bioreactor cartridge according to claim 2, comprising the steps of:
step A1: pinching off the end of the swab from which the sample is collected into the swab head receiving cavity, closing the opening of the swab head receiving cavity;
step A2: closing the first through hole and the third channel, opening the second through hole, moving the piston downwards, and naturally flowing the cleaning liquid in the storage cavity into the mixing cavity from the second channel;
step A3: closing the second through hole, opening the first through hole, moving the piston upwards, spraying the cleaning solution in the mixing cavity into the swab head receiving cavity through the first channel to soak the fiber substance containing the sample, and moving the piston downwards after the cleaning solution in the mixing cavity is completely sprayed into the swab head receiving cavity through the first channel, so that the cleaning solution in the swab head receiving cavity flows back into the mixing cavity;
step A4: repeating the step A3 for a certain number of times;
step A5: and closing the first through hole and opening the second through hole, moving the piston upwards, and enabling the cleaning liquid in the mixing cavity to flow into the storage cavity through the second channel to finish the collection of the cleaning liquid.
9. A method for cleaning a swab in a bioreactor cartridge according to claim 3, comprising the steps of:
step B1: pinching off the end of the swab from which the sample is collected, the swab head passing through the swab head receiving chamber and being immersed in the washing liquid in the mixing chamber, closing the opening of the swab head receiving chamber;
and step B2: closing the second through hole, the third channel and opening the first through hole, and reciprocating the piston between the swab head and the inclined through hole for a certain number of times;
and step B3: opening the second through hole, moving the piston downwards until the inclined through hole is exposed, and naturally flowing the cleaning solution into the storage cavity along the inclined through hole to finish the collection of the sample cleaning solution;
and step B4: the piston moves upwards and is blocked at the inclined through hole.
10. The cleaning method according to claim 8 or 9, wherein the third channel is externally connected to the detection device, and the collected cleaning solution flows into the externally connected device through the third channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211401936.6A CN115572662A (en) | 2022-11-10 | 2022-11-10 | Swab cleaning device in biological reaction card box and cleaning method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211401936.6A CN115572662A (en) | 2022-11-10 | 2022-11-10 | Swab cleaning device in biological reaction card box and cleaning method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115572662A true CN115572662A (en) | 2023-01-06 |
Family
ID=84588419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211401936.6A Pending CN115572662A (en) | 2022-11-10 | 2022-11-10 | Swab cleaning device in biological reaction card box and cleaning method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115572662A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112342128A (en) * | 2020-11-11 | 2021-02-09 | 苏州雅睿生物技术有限公司 | Nucleic acid extraction amplification test tube and working method thereof |
| CN212713521U (en) * | 2020-06-04 | 2021-03-16 | 天津康圣达医学检验实验室有限公司 | Gene sample collector, gene sample extractor and gene sample acquisition device |
| CN214636520U (en) * | 2021-04-14 | 2021-11-09 | 沈阳市妇婴医院 | Micro reagent mixing sample injector |
| CN217244511U (en) * | 2022-04-24 | 2022-08-23 | 长春西诺生物科技有限公司 | EP tube for collecting swab samples |
| CN217659945U (en) * | 2022-01-28 | 2022-10-28 | 郑州大学第一附属医院 | Improvement type pharynx swab collection subassembly |
-
2022
- 2022-11-10 CN CN202211401936.6A patent/CN115572662A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN212713521U (en) * | 2020-06-04 | 2021-03-16 | 天津康圣达医学检验实验室有限公司 | Gene sample collector, gene sample extractor and gene sample acquisition device |
| CN112342128A (en) * | 2020-11-11 | 2021-02-09 | 苏州雅睿生物技术有限公司 | Nucleic acid extraction amplification test tube and working method thereof |
| CN214636520U (en) * | 2021-04-14 | 2021-11-09 | 沈阳市妇婴医院 | Micro reagent mixing sample injector |
| CN217659945U (en) * | 2022-01-28 | 2022-10-28 | 郑州大学第一附属医院 | Improvement type pharynx swab collection subassembly |
| CN217244511U (en) * | 2022-04-24 | 2022-08-23 | 长春西诺生物科技有限公司 | EP tube for collecting swab samples |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20200277558A1 (en) | Systems and methods for cell culture device interconnection and fluidic device interconnection | |
| CN104745452B (en) | Rare cell automates capture device | |
| CN104203412B (en) | The disposable chip cartridges system of integrated form for chemistry and/or the portable multi parameter analysis of biological substance | |
| EP3482189A1 (en) | Automated microscopic cell analysis | |
| US11590496B2 (en) | Automated microscopic cell analysis | |
| JP6503355B2 (en) | Filter structure with slider valve and method for using it | |
| JP5881621B2 (en) | Multi-reactor box for dynamic cell culture | |
| CN109022274A (en) | Micro-fluidic chip, chip steerable system, micro fluidic device and method for extracting nucleic acid | |
| WO2010022391A2 (en) | Integrated, automated system for the study of cell and tissue function | |
| JP2019525139A (en) | Screening kit and method | |
| CN112538414A (en) | Full-sealed intelligent nucleic acid extraction device with micro-fluidic structure | |
| US7494809B2 (en) | Automated cell sample enrichment preparation method | |
| CN109266518A (en) | It is a kind of to be provided with micro-fluidic or nanometer flow control structure biological reaction apparatus | |
| CN107530704A (en) | Cultivate groove | |
| CN214937358U (en) | Full-sealed intelligent nucleic acid extraction device with micro-fluidic structure | |
| CN105518463B (en) | For handling the automatic mode and automation equipment of more parts of cell suspensions | |
| US11578303B2 (en) | Method and system for processing a biological sample | |
| CN115572662A (en) | Swab cleaning device in biological reaction card box and cleaning method thereof | |
| CN108579829A (en) | Exempt from pump type micro-fluidic chip and preparation method thereof and portable biochemical analyser | |
| CN109211737A (en) | Sample testing apparatus, sample analyser and sample testing method | |
| CN103608116A (en) | Processing of biological sample components | |
| JPS62110158A (en) | Sampler and method thereof | |
| KR102458032B1 (en) | Rt-pcr device | |
| US20200292541A1 (en) | Whole blood staining preparation cartridge and system | |
| US20110215257A1 (en) | Separation Device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20230106 |