CN115521768B - Oil displacement system and method for improving crude oil recovery ratio of high-freezing oil reservoir - Google Patents
Oil displacement system and method for improving crude oil recovery ratio of high-freezing oil reservoir Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/588—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific polymers
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Materials Engineering (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Geology (AREA)
- Mining & Mineral Resources (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Fluid Mechanics (AREA)
- Geochemistry & Mineralogy (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The application particularly relates to an oil displacement system and a method for improving the recovery ratio of crude oil in a high-freezing-point oil reservoir, which belong to the technical field of oil exploitation, wherein the oil displacement system comprises a microorganism oil displacement system and a chemical oil displacement system; the microbial oil displacement system comprises a mixture of hydrocarbon degrading bacteria and water, wherein the hydrocarbon degrading bacteria at least comprise one hydrocarbon degrading bacteria, so that the high-freezing oil blocking the pore throat of a reservoir after microbial degradation and solidification can be used for improving the fluidity of crude oil, and the swept volume and the oil washing efficiency can be improved through a low-concentration weak base ternary system, so that the recovery ratio of a high-freezing oil reservoir is greatly improved compared with that of water flooding; the system has obvious functions of reducing pour point, viscosity and wax precipitation prevention on crude oil; the micro-molecular biological surfactant produced by microorganism metabolism in the system plays a role in synergy with the surfactant, reduces the tension of an oil-water interface, enhances the oil washing capability, and can greatly improve the crude oil recovery ratio.
Description
Technical Field
The application belongs to the technical field of petroleum exploitation, and particularly relates to an oil displacement system and method for improving the recovery ratio of crude oil in a high-freezing-point oil reservoir.
Background
Through indoor research, field practice and application of a polymer/alkali/surfactant multi-element compound chemical system for decades, a set of mature oil displacement system research and development and preparation technology is formed, the problem that oil field cannot enter high-water-content later-stage underground residual oil production is effectively solved, and important contribution is made to further improvement of recovery ratio of the oil field. However, the chemical system has certain limitations for special oil reservoirs such as high pour point oil reservoirs. The oil product of the oil reservoir has the characteristics of high solidifying point, high wax content and high wax precipitation temperature, and has poor fluidity and larger exploitation difficulty than thin oil. The high-pour-point oil reservoirs are distributed all over the world, the distribution of Liaohe oil fields is most concentrated, and the high-pour-point oil reservoirs are the largest high-pour-point oil production bases nationwide, currently generally enter a high-water-content development stage, have low exploitation speed and low overall recovery ratio. Through years of indoor experiments, weak base ternary complex flooding is realized at present, and considerable crude oil recovery ratio can be improved.
At present, in the petroleum exploitation site, a part of common oil reservoirs use a microbial single-well huff and puff technology for treating wax deposition in a near-wellbore zone, namely, a small amount of microbial solution is injected from a crude oil extraction end, and the oil reservoirs are extracted from the same well after being stewed, so that a certain effect is achieved.
Disclosure of Invention
Applicants found during the course of the application that: through water injection development for many years, the temperature of an oil reservoir is reduced to different degrees, high-freezing oil forms a solidification state in a near-well low-temperature area, the permeability is reduced by blocking a stratum, and the speed of crude oil flowing into a shaft is influenced, so that the oil production capacity is influenced, and common chemical oil displacement is difficult to solve; meanwhile, when the single-well throughput technology of microorganisms is adopted, the injection quantity cannot be too large, because the microorganism carrying medium is water, a large amount of water is injected from the extraction well to push crude oil to a far place, and the recovery period is long; the method has the advantages that no main technology is matched, the amount of crude oil passing through the untangling holes is limited, and the overall benefit is low.
The present application has been made in view of the above problems, and has as its object to provide a flooding system and method for enhanced oil recovery from a high pour point oil reservoir that overcomes or at least partially solves the above problems.
The embodiment of the application provides an oil displacement system for improving the crude oil recovery ratio of a high-freezing oil reservoir, which comprises a microorganism oil displacement system and a chemical oil displacement system;
the microbial oil displacement system comprises: a mixture of hydrocarbon degrading bacteria and water, the hydrocarbon degrading bacteria comprising at least one hydrocarbon degrading bacteria.
Optionally, the hydrocarbon degrading bacteria of the hydrocarbon degrading bacterial agent are obtained by carrying out bacteria enrichment and screening on oil well produced liquid of a high-freezing oil reservoir to be displaced.
Optionally, the hydrocarbon degrading bacterial agent has a bacterial content of 10 7 cfu/mL-10 8 cfu/mL；
In the microbial oil displacement system, the weight concentration of the hydrocarbon degrading bacterial agent is 4500mg/L-5500mg/L.
Optionally, the microbial flooding system further comprises a microbial activator; the microbial activator comprises a mixture of corn steep liquor dry powder, sodium nitrate, diammonium phosphate and yeast powder.
Optionally, in the microbial oil displacement system, the weight concentration of the corn steep liquor dry powder is 500mg/L-1500mg/L; the weight concentration of the sodium nitrate is 500mg/L-1500mg/L; the weight concentration of the diammonium hydrogen phosphate is 500mg/L-1500mg/L; the weight concentration of the yeast powder is 200mg/L-300mg/L.
Alternatively, the chemical flooding system comprises a weak base ternary system comprising a mixture of anionic surfactant, polymer, sodium carbonate and water.
Optionally, the anionic surfactant is a petroleum sulfonate surfactant and the polymer is a first partially hydrolyzed polyacrylamide;
in the weak base ternary system, the weight concentration of the petroleum sulfonate surfactant is 500mg/L-3000mg/L, the weight concentration of the first partially hydrolyzed polyacrylamide is 1000mg/L-2000mg/L, and the weight concentration of the sodium carbonate is 500mg/L-4000mg/L.
Optionally, the chemical flooding system further comprises a pre-slug liquid and a protection slug liquid.
Optionally, the pre-slug liquid is second partially hydrolyzed polyacrylamide, and the weight concentration of the second partially hydrolyzed polyacrylamide is 2000-3000mg/L; the viscosity of the second partially hydrolyzed polyacrylamide is 100-200 mpa.s;
the protection slug liquid is third partially hydrolyzed polyacrylamide, and the weight concentration of the third partially hydrolyzed polyacrylamide is 800mg/L-2000mg/L; the viscosity of the third partially hydrolyzed polyacrylamide is 50-150 mpa.s.
Based on the same inventive concept, the embodiment of the application also provides an oil displacement method for improving the recovery ratio of crude oil of a high-freezing oil reservoir, which comprises the following steps:
sequentially injecting a microorganism oil displacement system and a chemical oil displacement system of the oil displacement system into a high-freezing-point oil reservoir for oil displacement treatment so as to improve the crude oil recovery ratio of the high-freezing-point oil reservoir;
the microbial oil displacement system comprises: a mixture of hydrocarbon degrading bacterial agent and water.
Optionally, the microbial oil displacement system and the chemical oil displacement system of the oil displacement system are sequentially injected into the high-freezing-point oil reservoir for oil displacement treatment so as to improve the recovery ratio of crude oil of the high-freezing-point oil reservoir,
the chemical flooding system includes a weak base ternary system including a mixture of anionic surfactant, polymer, sodium carbonate, and water.
Optionally, the microbial oil displacement system and the chemical oil displacement system of the oil displacement system are sequentially injected into the high-freezing-point oil reservoir for oil displacement treatment so as to improve the recovery ratio of crude oil of the high-freezing-point oil reservoir,
the chemical oil displacement system also comprises a preposed slug liquid and a protective slug liquid;
the injection of the chemical flooding system specifically comprises the following steps: and sequentially injecting the pre-slug liquid, the weak base ternary system and the protection slug liquid into the high-freezing-point oil reservoir.
Optionally, the injection volume of the microbial oil displacement system accounts for 10% -20% of the total pore volume of the underground oil-containing layer; the injection volume of the pre-slug liquid accounts for 5-15% of the total pore volume of the underground oil-bearing layer; the injection volume of the weak base ternary system accounts for 75% -85% of the total pore volume of the underground oil-bearing layer; the injection volume of the protection slug liquid accounts for 5% -15% of the total pore volume of the underground oil-bearing layer.
One or more technical solutions in the embodiments of the present application at least have the following technical effects or advantages:
the oil displacement system for improving the crude oil recovery ratio of the high-freezing-point oil reservoir comprises a microorganism oil displacement system and a chemical oil displacement system; the microbial oil displacement system comprises: the hydrocarbon degrading bacteria agent at least comprises a hydrocarbon degrading bacteria, and the hydrocarbon degrading bacteria can generate micromolecular biosurfactant, so that the high-freezing oil blocking the pore throat of a reservoir after microbial degradation and solidification can be used for improving the fluidity of crude oil, and the swept volume and the oil washing efficiency can be improved through a low-concentration weak base ternary system, so that the recovery ratio of the high-freezing oil reservoir is greatly improved compared with that of a water flooding; the system has obvious functions of reducing pour point, viscosity and wax precipitation prevention on crude oil; the micro-molecular biological surfactant produced by microorganism metabolism in the system plays a role in synergy with the surfactant, reduces the tension of an oil-water interface, enhances the oil washing capability, and can greatly improve the crude oil recovery ratio.
The foregoing description is only an overview of the present application, and is intended to be implemented in accordance with the teachings of the present application in order that the same may be more clearly understood and to make the same and other objects, features and advantages of the present application more readily apparent.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a method provided by an embodiment of the present application;
FIG. 2 is a schematic diagram of components of an oil displacement system provided by an embodiment of the application;
FIG. 3 is a schematic illustration of a pre-slug formulation provided by an embodiment of the present application;
FIG. 4 is a schematic illustration of a main slug formulation provided by an embodiment of the present application;
FIG. 5 is a schematic illustration of a protected slug formulation provided by an embodiment of the present application;
FIG. 6 is a schematic diagram of a slug combination of a flooding system provided by an embodiment of the present application;
FIG. 7 is a graph of the results of a single colony streaking experiment provided by an embodiment of the present application;
FIG. 8 is a graph showing the result of amplification of the AlkB gene of the strain according to the example of the present application;
FIG. 9 is a phylogenetic analysis of strains according to an embodiment of the present application;
FIG. 10 is a technical roadmap for research of nutritional activators provided by an embodiment of the application;
FIG. 11 is a graph of water-in-oil displacement versus pressure-in-oil displacement efficiency for scheme 1 provided in example 3 of the present application;
FIG. 12 is a graph of water-in-oil displacement versus pressure-in-oil displacement efficiency for scheme 2 provided in example 3 of the present application;
FIG. 13 is a graph of water-in-oil displacement versus pressure-in-oil displacement efficiency for scheme 3 provided in example 3 of the present application;
FIG. 14 is a graph of water-in-oil displacement versus pressure-in-oil displacement efficiency for scheme 4 provided in example 3 of the present application;
FIG. 15 is a graph of flooding water-pressure-flooding efficiency for scheme 5 provided in example 3 of the present application.
Detailed Description
The advantages and various effects of the present application will be more clearly apparent from the following detailed description and examples. It will be understood by those skilled in the art that these specific embodiments and examples are intended to illustrate the application, not to limit the application.
Throughout the specification, unless specifically indicated otherwise, the terms used herein should be understood as meaning as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. In case of conflict, the present specification will control.
Unless otherwise specifically indicated, the various raw materials, reagents, instruments, equipment and the like used in the present application are commercially available or may be prepared by existing methods.
The technical scheme of the embodiment of the application aims to solve the technical problems, and the overall thought is as follows:
the high-pour-point oil reservoirs are distributed all over the world, the distribution of Liaohe oil fields is most concentrated, the high-pour-point oil reservoirs are the largest production base of the high pour-point oil in the whole country, the high-water-content development stage is generally carried out at present, the exploitation speed is low, the overall recovery ratio is low, the existing method is to improve the original recovery ratio through a weak base ternary composite oil displacement system, and the applicant finds that in the application process: through water injection development for many years, the temperature of an oil reservoir is reduced to different degrees, high-freezing oil forms a solidification state in a near-well low-temperature area, the permeability is reduced by blocking a stratum, and the speed of crude oil flowing into a shaft is influenced, so that the oil production capacity is influenced, and common chemical oil displacement is difficult to solve; in the petroleum exploitation field, a part of common oil reservoirs use a microbial single-well huff-puff technology for treating wax deposition in a near wellbore zone, namely, a small amount of microbial solution is injected from a crude oil extraction end, and the oil reservoirs are extracted from the same well after well stewing, so that a certain effect is obtained, and the applicant finds that in the application process: when the single-well microbial huff and puff technology is adopted, the injection quantity cannot be too large, because the microorganism carrying medium is water, a large amount of water injected from the extraction well can push crude oil to a distance, and the extraction period is long; the method has the advantages that no main technology is matched, the amount of crude oil passing through the untangling holes is limited, and the overall benefit is low.
According to an exemplary embodiment of the present application, there is provided an oil displacement system for enhanced oil recovery from a high pour point oil reservoir, the oil displacement system comprising a microbial oil displacement system and a chemical oil displacement system;
the microbial oil displacement system comprises: a mixture of hydrocarbon degrading bacteria and water, the hydrocarbon degrading bacteria comprising at least one hydrocarbon degrading bacteria.
The applicant provides a microbial chemical compound oil displacement system for improving the recovery ratio of a high-pour-point oil reservoir through extensive comparative research and repeated experiments, which can degrade and solidify high pour-point oil blocking the pore throat of the reservoir by microorganisms to improve the fluidity of crude oil, and can improve the swept volume and the wash oil efficiency by a low-concentration weak base ternary system, so that the problem of poor fluidity of the blocked pore throat after solidification of the high pour-point oil is solved, the problem of low throughput efficiency of individual microorganisms is also solved, after the advantages of the high pour-point oil and the low pour-point oil are effectively combined, the system plays an obvious roles of pour-point reduction, viscosity reduction and wax precipitation prevention on the crude oil, meanwhile, the micro-molecular biosurfactant produced by microbial metabolism plays a role of synergism with the surfactant, reduces the interfacial tension of oil water, enhances the wash oil capability, greatly improves the recovery ratio of the high pour-point oil, opens up a high-point oil tertiary oil recovery, and opens up a high-efficiency path for the three-time oil recovery of the high pour-point oil, wherein the micro-molecular biosurfactant is proteoglycan and lipopeptide, and the micro-molecular biosurfactant plays a role of synergism: (1) The small molecular biological surfactant is resistant to high temperature, so that the long-term thermal stability of the surfactant is improved; (2) The small molecular biological surfactant reduces the adsorption quantity of polymer and petroleum sulfonate, and improves the action effect of the surfactant in the ground.
As an optional implementation mode, the hydrocarbon degrading bacteria of the hydrocarbon degrading bacteria are obtained by carrying out microorganism enrichment and screening on oil well produced liquid of a high-freezing oil reservoir to be displaced.
Specifically, the method for obtaining the hydrocarbon degrading bacterial agent comprises the following steps:
s1, obtaining oil well produced liquid, wherein the oil well produced liquid is preferably the oil well produced liquid of a high-freezing oil reservoir to be displaced;
s2, enriching microorganisms in the oil well produced liquid to obtain oil well microorganisms;
s3, screening the oil well microorganisms through a hydrocarbon degrading bacterium solid paraffin culture medium to obtain a plurality of hydrocarbon degrading bacteria;
s4, testing hydrocarbon degradation performance of a plurality of hydrocarbon degradation bacteria, and then preparing a plurality of hydrocarbon degradation bacteria with good hydrocarbon degradation performance into a bacterial agent to obtain the hydrocarbon degradation bacteria bacterial agent, wherein the hydrocarbon degradation bacteria bacterial agent can be bacterial agents comprising a plurality of hydrocarbon degradation bacteria or bacterial agents of single hydrocarbon degradation bacteria.
During actual operation, firstly, enriching microorganisms in an oil well by using an oil well produced fluid enrichment culture medium, and then, selectively screening strains with hydrocarbon degradation function by using a hydrocarbon degradation bacterium solid paraffin culture medium, wherein the raw materials of the hydrocarbon degradation bacterium solid paraffin culture medium comprise: naCl 3g/L, K 2 HPO 4 0.1g/L、KH 2 PO 4 0.5g/L、MgSO 4 ·7H 2 O 0.4g/L、FeSO 4 ·7H 2 O 0.01g/L、CaCl 2 0.01g/L、NaNO 3 3g/L, 0.5g/L of yeast extract and 2g/L of solid paraffin, wherein the pH value is 7.0-7.2; the raw materials for enriching the oil well produced fluid culture medium comprise: naCl 3g/L, K 2 HPO 4 0.1g/L、KH 2 PO 4 0.5g/L、MgSO 4 ·7H 2 O 0.4g/L、FeSO 4 ·7H 2 O 0.01g/L、CaCl 2 0.01g/L、NaNO 3 3g/L, 0.5g of yeast extract, and pH of 7.0-7.2; the screening method specifically comprises the following steps: enriching the oil well output mixed solution (5% of V/V), inoculating the oil well output mixed solution to a hydrocarbon degrading bacterium solid paraffin culture medium, standing and culturing for 4-5 days in a 70 ℃ incubator, and observing the degradation condition of the solid paraffin; the performance optimization method specifically comprises the following steps: (1) Picking single colony with sterilized toothpick, adding 20 μl of deionized sterile water into PCR tube (200 μl), washing with pipetting gun, mixing (if thallus adheres to tube wall, flicking centrifuge tube with finger), boiling the PCR tube in boiling water for 10 min, freezing in refrigerator for 2 min, centrifuging at 1000rpm for 1 min, and sucking 5 μl of supernatant as DNA amplification stock solution. (2) PCR reaction System: 5 mu L of DNA amplification stock solution; 10 XPCR buffer (containing magnesium ions) 5. Mu.L; dNTP mixed nucleotide (10 mmol/L) 1. Mu.L; left primer (25. Mu. Mol/L) 1. Mu.L; right primer (25. Mu. Mol/L); 1. Mu.LEX-Taq enzyme (2 units per microliter) 0.5. Mu.L; the total volume was made up to 50. Mu.L with deionized water. (3) PCR amplification reaction procedure: first, 95 degrees of pre-denaturation for 10 minutes; next, the main cycle was continued 30 times (94 degrees 30 seconds, functional gene primer annealing temperature 45 seconds, 72 degrees 30 seconds); finally, the amplified product is extended at 72 ℃ for 7 minutes; the product was taken out of the PCR apparatus and stored at 4 ℃. (4) electrophoresis detection: gel with concentration of 8g/L is prepared by using 0.5 XTBE buffer for hot-melting agarose, micro Goldview (with concentration of about 0.2 mu g/ml) is added, 3 mu L of PCR amplified product is mixed with 0.5 mu L-6 times of loading buffer, 3.5 mu L of each sample is loaded, 120V/m electrophoresis is carried out for 40min, an electrophoresis result is shot by an ultraviolet gel imaging system, and the first few microorganisms with the best performance in the performance test are screened out as bacterial agents according to actual conditions, and 2-4 microorganisms are generally selected.
As an alternative embodiment, the hydrocarbon degrading bacterial agent has a bacterial content of 10 7 cfu/mL-10 8 cfu/mL；
In the microbial oil displacement system, the weight concentration of the hydrocarbon degrading bacterial agent is 4500mg/L-5500mg/L.
The concentration of the hydrocarbon degrading bacterial agent is controlled to be 4500mg/L-5500mg/L, and the specific operation is that the concentration is determined according to the recovery ratio of the object model experiment under different microorganism system concentrations, and the adverse effect of the overlarge concentration is that the cost is overhigh, and the adverse effect of the overlarge concentration is that the recovery ratio is low.
As an alternative embodiment, the microbial flooding system further comprises a microbial activator comprising: corn steep liquor dry powder, sodium nitrate, diammonium phosphate and yeast powder; the concentration of the corn steep liquor dry powder is 500mg/L-1500mg/L; the concentration of sodium nitrate is 500mg/L-1500mg/L; the concentration of diammonium hydrogen phosphate is 500mg/L-1500mg/L; the concentration of the yeast powder is 200mg/L-300mg/L.
The corn steep liquor dry powder is controlled to have the concentration of 500mg/L-1500mg/L, the sodium nitrate is controlled to have the concentration of 500mg/L-1500mg/L, the diammine hydrogen phosphate is controlled to have the concentration of 500mg/L-1500mg/L, and the yeast powder is controlled to have the concentration of 200mg/L-300mg/L, so that the normal growth of microorganisms is ensured, meanwhile, the chemical flooding system is not influenced, the viscosity of the chemical flooding system is greatly reduced due to the adverse effect of the excessive concentration, the sweep coefficient of the system is influenced, the microbial system cannot be completely activated, the wax component in crude oil cannot be effectively degraded, and the sufficient biosurfactant cannot be generated;
as an alternative embodiment, the chemical flooding system comprises a weak base ternary system comprising a mixture of anionic surfactant, polymer and sodium carbonate;
the anionic surfactant is petroleum sulfonate surfactant, and the polymer is first partially hydrolyzed polyacrylamide;
in the weak base ternary system, the weight concentration of the petroleum sulfonate surfactant is 500mg/L-3000mg/L, the weight concentration of the first partially hydrolyzed polyacrylamide is 1000mg/L-2000mg/L, the degree of hydrolysis is 20% -27%, the molecular weight is 1900-2200 ten thousand, and the weight concentration of the sodium carbonate is 500mg/L-4000mg/L.
The reason that hydrocarbon degrading bacteria can survive in a chemical oil displacement system is that the screened microbial inoculum is greatly influenced by alkali, namely sodium carbonate, in the chemical oil displacement system, and the microbial is separated from the oil displacement slug by using the partially hydrolyzed polyacrylamide pre-slug, so that the hydrocarbon degrading bacteria are ensured to survive in the oil displacement system.
According to another exemplary embodiment of the application, a method for preparing an oil displacement system for improving the recovery ratio of crude oil in a high-freezing oil reservoir is provided; the method comprises the following steps: the system preparation takes water as a dispersion medium, (1) a microbial agent, a microbial activator and water are used for preparing a microbial system; (2) Preparing a polymer into a mother solution with the concentration of 3000mg/L to 5000 mg/L; (3) Preparing surfactant into mother solution with concentration of 5000-10000 mg/L; (4) Preparing sodium carbonate into 20000mg/L-50000mg/L mother liquor; (5) Mixing and stirring the polymer mother liquor, the surfactant mother liquor, the sodium carbonate mother liquor and water together for 2 hours according to the proportion; after the mother solution is prepared, when in use, the mother solution is prepared into proper use concentration according to the requirement, and then the mother solution is injected into an underground oil layer.
According to another exemplary embodiment of the present application, there is provided a method of displacing oil for increasing recovery of crude oil from a high pour point oil reservoir, the method comprising:
sequentially injecting a microorganism oil displacement system and a chemical oil displacement system of the oil displacement system into a high-freezing-point oil reservoir for oil displacement treatment so as to improve the crude oil recovery ratio of the high-freezing-point oil reservoir;
the microbial oil displacement system comprises: the chemical oil displacement system comprises a weak base ternary system, a pre-slug liquid and a protection slug liquid. The method comprises the steps of carrying out a first treatment on the surface of the
The specific operation is as follows:
s1, injecting the microbial oil displacement system into a subsurface oil-containing layer, wherein the microbial oil displacement system is commonly called a microbial slug by a person skilled in the art;
s3, injecting the pre-slug liquid into the underground oil-bearing layer, wherein the pre-slug liquid is usually called as a pre-slug by a person skilled in the art;
s4, injecting the weak base ternary system into a subsurface oil-bearing layer, wherein the weak base ternary system is commonly called a main slug by a person skilled in the art;
s5, injecting the protection slug liquid into the underground oil-bearing layer, wherein the protection slug liquid is generally called a protection slug by a person skilled in the art;
the microbial slugs, the pre-slug, the main slug and the protection slug are sequentially injected from the surface to the underground oil-water mixing belt of the containing layer and finally mixed in the oil-water mixing belt.
Specifically, the injection volume of the microbial oil displacement system accounts for 10% -20% of the total pore volume of the underground oil-bearing layer; the injection volume of the pre-slug liquid accounts for 5-15% of the total pore volume of the underground oil-bearing layer; the injection volume of the weak base ternary system accounts for 75% -85% of the total pore volume of the underground oil-bearing layer; the injection volume of the protection slug liquid accounts for 5% -15% of the total pore volume of the underground oil-bearing layer; it should be noted that the total pore volume of the subsurface oil-bearing layer is obtained by methods commonly used in the industry at present.
Controlling the injection amount of a microorganism system to account for 10% -20% of the total pore volume in a stratum, determining according to an improvement value of a recovery ratio of a physical simulation experiment in actual use, wherein the adverse effect of the excessive injection amount is that economic evaluation is influenced, the cost is high, the input-output ratio is influenced, the adverse effect of the insufficient injection amount is that the wax component in the high-freezing oil is not effectively degraded, and meanwhile, a sufficient amount of biosurfactant cannot be generated;
the reason for controlling the injection amount of the front slug to account for 5-15% of the total pore volume in the stratum is to ensure that the front slug can effectively block the hypertonic layer, the adverse effect of the excessive injection amount is that the stratum can be blocked, and the adverse effect of the excessive injection amount is that the hypertonic layer cannot be effectively blocked;
controlling the lifting injection amount of the weak base ternary system to account for 75% -85% of the total pore volume in the stratum, determining according to the improvement value of the recovery ratio of a physical simulation experiment in actual use, wherein the adverse effect of the excessive injection amount is to influence the economic evaluation, the cost is too high, the input-output ratio is influenced, and the adverse effect of the too small adverse effect is to not exert the effect of the oil displacement system in improving the sweep coefficient and the wash oil efficiency;
the reason for controlling the injection quantity of the protection slug accounting for 5-15% of the total pore volume in the stratum is that the oil displacement main slug is effectively protected from being diluted by the subsequent water drive, the adverse effect of the excessive injection quantity is that economic evaluation is influenced, the cost is too high, the input-output ratio is influenced, and the adverse effect of too small is that the oil displacement main slug and the subsequent water drive cannot be effectively separated;
in this embodiment, the pre-slug liquid injected into the stratum is preferably a polymer aqueous solution composed of a polymer and stratum water, wherein the concentration of the polymer in the polymer aqueous solution is 2000-3000mg/L, the viscosity of the polymer aqueous solution is 100-200 mPa.s, the polymer adopted by the pre-slug liquid is partially hydrolyzed polyacrylamide, the molecular weight of the partially hydrolyzed polyacrylamide is 3000 ten thousand, and the degree of hydrolysis is 23-27%.
In this embodiment, in the step of injecting the protection slug liquid into the formation, the protection slug liquid is preferably a polymer aqueous solution composed of a polymer and water, and the polymer concentration of the polymer aqueous solution used in the protection slug liquid is 0.08-0.20wt% (i.e., 800mg/L-2000 mg/L), the viscosity of the polymer aqueous solution is 50-150mpa·s, the polymer used in the protection slug liquid is partially hydrolyzed polyacrylamide, and the molecular weight of the partially hydrolyzed polyacrylamide is 2000 ten thousand, and the degree of hydrolysis is 23-27%.
The oil displacement system and method for improving the recovery ratio of crude oil in the high-freezing oil reservoir according to the application are described in detail below with reference to examples, comparative examples and experimental data.
Example 1
Preparation of the culture medium:
enriching oil well produced fluid culture medium (g/L):
NaCl 3g、K 2 HPO 4 0.1g、KH 2 PO 4 0.5g、MgSO 4 ·7H 2 O 0.4g、FeSO 4 ·7H 2 O 0.01g、CaCl 2 0.01g、NaNO 3 3g, 0.5g of yeast extract and pH 7.0-7.2;
hydrocarbon degrading bacteria solid paraffin culture medium (g/L):
NaCl 3g、K 2 HPO 4 0.1g、KH 2 PO 4 0.5g、MgSO 4 ·7H 2 O 0.4g、FeSO 4 ·7H 2 O 0.01g、CaCl 2 0.01g、NaNO 3 3g, 0.5g of yeast extract, 2g of solid paraffin, pH 7.0-7.2
Obtaining strains with excellent performance:
firstly enriching microorganisms in an oil well by using an oil well produced fluid enrichment culture medium, and then selectively screening strains with hydrocarbon degradation function by using a hydrocarbon degradation strain solid paraffin culture medium;
the specific screening method is as follows: and (3) enriching the oil well output mixed solution (5 percent, V/V), inoculating the oil well output mixed solution to a hydrocarbon degrading bacterium solid paraffin culture medium, standing and culturing for 4-5 days in a 70 ℃ incubator, and observing the degradation condition of the solid paraffin.
Coated LB plates for detection of samples 61-11, 64-14, 68-588 and 71-551, 10.25 in 2018 were subjected to sterilization, and single colony streaks were picked on 26.9 in 2018 (as shown in FIG. 7);
20 strains of bacteria selected in 10.26 of 2018 are inoculated into a culture medium for utilizing solid wax, and the solid wax distribution condition in the culture solution is observed in 9.28 of 2018, wherein the solid wax particles of 61-11-2 (all fragmented), 71-511-1, 61-11-3, 61-11-4 and 68-588 are better in distribution, and the solid wax particles are slightly larger in flake; 61-11-1 and 71-551-2 have some solid wax particles, and large flake solid wax exists; the solid wax of the culture solution of the 64-14 strain is unchanged.
The hydrocarbon degradation function of the strain is verified by functional gene-colony PCR, and the specific steps are as follows:
(1) Picking single colony with sterilized toothpick, adding 20 μl of deionized sterile water into PCR tube (200 μl), washing with pipetting gun, mixing (if thallus adheres to tube wall, flicking centrifuge tube with finger), boiling the PCR tube in boiling water for 10 min, freezing in refrigerator for 2 min, centrifuging at 1000rpm for 1 min, and sucking 5 μl of supernatant as DNA amplification stock solution.
(2) PCR reaction system: 5 mu L of DNA amplification stock solution; 10 XPCR buffer (containing magnesium ions) 5. Mu.L; dNTP mixed nucleotide (10 mmol/L) 1. Mu.L; left primer (25. Mu. Mol/L) 1. Mu.L; right primer (25. Mu. Mol/L); 1. Mu.L of EX-Taq enzyme (2 units per microliter) 0.5. Mu.L; the total volume was made up to 50. Mu.L with deionized water.
(3) PCR amplification reaction procedure: first, 95 degrees of pre-denaturation for 10 minutes; next, the main cycle was continued 30 times (94 degrees 30 seconds, functional gene primer annealing temperature 45 seconds, 72 degrees 30 seconds); finally, the amplified product is extended at 72 ℃ for 7 minutes; the product was taken out of the PCR apparatus and stored at 4 ℃.
(4) And (3) electrophoresis detection: gel with a concentration of 8g/L was prepared by thermally dissolving agarose in 0.5 XTBE buffer, adding a trace amount of Goldview (with a concentration of about 0.2. Mu.g/ml), mixing 3. Mu.L of PCR amplification product with 0.5. Mu.L-6 times of loading buffer, loading 3.5. Mu.L of each sample, electrophoresis at 120V/m for 40min, and taking electrophoresis results by an ultraviolet gel imaging system.
Alkane oxygenases initiate aerobic biodegradation of alkanes. The most currently studied alkane oxygenase is the first AlkB found in Pseudomonas putida, which is widely present in a variety of crude oil degrading bacteria, and up to now over 300 different types of AlkB genes have been detected in at least 50 bacteria. Therefore, the gene can be used as an important reference index for analyzing whether hydrocarbon degrading bacteria exist in a sample and the diversity of the hydrocarbon degrading bacteria. The hydrocarbon degrading function of the strain was verified by PCR of the alkB gene in the genome of the hydrocarbon degrading strain (shown in the following Table) using the alkB gene primer, and the result is shown in FIG. 8. The figure shows that the 23 hydrocarbon degradation strains all contain the alkB genes, which proves the high efficiency and reliability of the hydrocarbon degradation strain screening method adopted in the research.
The hydrocarbon degrading bacteria are screened to obtain 20 strains by using a solid paraffin culture medium of the hydrocarbon degrading bacteria, and the strains can grow by taking the high-freezing oil as a unique carbon source. The strains are now collectively numbered (H65-1-H65-20) and the results of the amplification of the hydrocarbon degradation functional genes are summarized in the following table:
and (3) strain identification system development and adaptability analysis:
the strain identification method developed recently is based on strain character description, and mainly uses indexes such as strain morphology, growth metabolism, physiological and biochemical characteristics and the like to classify and identify strains, and particularly refers to a Berger's bacteria classification manual. Although the taxonomic method can perform preliminary identification on the screened microorganisms according to a large number of character descriptions, the method has a plurality of indexes, is large in workload and is not completely reliable. Modern developed classification methods based on molecular biology classify species according to conserved genes such as the 16S ribosomal RNA gene. Because the evolution rate of the conserved gene is relatively slow, the conserved gene can be kept stable under different environments, and can be used as a target gene for strain classification and identification. The research firstly carries out identification of genus level on the isolated oil extraction functional bacteria according to the highly conserved 16S rRNA gene, then downloads a reference sequence according to blast results to construct a phylogenetic tree, and presumes possible species level classification of the strain.
The analysis result of the system development of the high-temperature resistant oil extraction functional bacteria of the high-freezing oil reservoir constructed based on the 16S sequence in the study is shown in fig. 9:
according to the identification result, 20 hydrocarbon degrading bacteria are totally separated from the high-freezing oil reservoir, wherein the bacteria mainly comprise bacillus, including bacillus subtilis, bacillus licheniformis, anaerobic bacillus, geobacillus and the like. The adaptability analysis of hydrocarbon degrading bacteria oil deposit is mainly evaluated by examining the growth and metabolism activity of the bacteria under different temperature, mineralization degree and oxygen condition (without oxygen condition), and the adaptability analysis results of 20 oil extraction functional bacteria in the bacteria library are shown in the following table.
The strains with excellent performance are microorganism H65-4 and microorganism H65-6.
Example 2
Indoor activated formula system research is carried out on the basis of analysis of early-stage endogenous microorganisms (functional bacteria count analysis and microbial community composition analysis). The following principles apply to the screening of the activator formulation: (l) Contains nutrients lacking in the growth of microorganisms in the oil reservoir, including inorganic nutrients and organic nutrients. The oil reservoir environment generally lacks nutrition required by the growth of microorganisms, and the lacking main nutrition type can be primarily known by analyzing the chemical components in the stratum produced liquid, so that a foundation is laid for selecting a proper nutrition activator. (2) Can selectively activate microbial communities in oil reservoirs, which are beneficial to oil recovery. (3) the activator component must be compatible with the injected water and formation water. The activator must have good solubility, and when it contacts with the formation water, it will not react chemically, and it will not precipitate, because the bacteria only use the dissolved nutrients, and if the activator reacts with the formation water, it will cause the formation to block. (4) The main ingredients of the activator formulation must be inexpensive and widely available.
The research thought and the technical route of the nutrition activating agent of the oil extraction functional bacteria are shown in figure 10.
Screening carbon as a hydrocarbon degrading bacterium nutrition activator:
10 strains of bacteria are selected by comprehensively considering indexes such as high-freezing oil emulsification grade, 2% high-freezing oil degradation, crude oil degradation functional genes, viable ternary system and the like, and are respectively: h65-1, 3, 4, 6, 8, 13, 15, 16, 18 and 19, developing carbon source screening, namely 0.3 percent molasses (1)/0.8 percent corn steep liquor dry powder (2)/0.8 percent liquid paraffin (3)/0.8 percent vegetable oil (4) +0.2 percent sodium nitrate+0.2 hydrogen diamine phosphate+0.02 percent yeast powder, 10 percent inoculation amount, culturing at 65 ℃ and 120rmp for 48 hours, and observing the growth and metabolism conditions of the strain.
As shown in the following table, the results of the experiment show that the cell growth was good in the formulation using molasses and corn steep liquor as carbon sources, but the cell growth was poor and almost no in the formulation using liquid paraffin and food oil as carbon sources, and the OD600 values of the molasses and corn steep liquor culture solutions were measured, and the overall growth promoting effect corn steep liquor dry powder was better than molasses.
Remarks: "a, +" indicates that there is cell growth, but little. "-" means aseptic growth.
When the growth of the bacterial cells using liquid paraffin and food oil as carbon sources was observed for 96 hours, the bacterial cells were grown with individual strains (H65-4, 8, 11, 16, 18, 19), the LB plates were diluted 104 and plated for counting, and the bacterial cells were not grown on the LB plates after 2 days of culture. All strains are separated from oil well produced water, but enrichment is enriched by adopting rich nutrition formulas such as yeast powder, molasses, peptone and the like (even if liquid wax is used as the only carbon source for enrichment, 0.1% of yeast powder is added into the culture medium), so that all strains are better utilized for molasses and corn steep liquor dry powder during carbon source screening. The comprehensive analysis experiment result shows that the corn steep liquor dry powder can be used as the optimal activator carbon source.
Screening nitrogen sources of hydrocarbon degrading bacteria nutrition activators:
and (3) determining the corn steep liquor dry powder as a carbon source, and evaluating the growth activity of 9 oil extraction functional bacteria (H65-1, 3, 4, 6, 8, 13, 15, 18 and 19) in different nitrogen source culture mediums. The formula of the culture medium is designed as follows: screening of 0.4% corn steep liquor dry powder+nitrogen source 0.2% sodium nitrate (1)/0.287% ammonium chloride (2)/0.161% urea (3)/0.354% diammonium phosphate (4) +0.02% yeast powder. The seed solution was inoculated at an amount of 10%, and cultured at 65℃and 120rmp for 48 hours. As a result, the cell growth was very poor and hardly observed in the formulation using sodium nitrate and ammonium chloride as nitrogen sources, while the cell growth was very poor using liquid urea and diammonium phosphate as nitrogen sources, and the OD of the culture solution was measured 600 The values, generally sodium nitrate, are the best nitrogen source, with the individual strains (H65-1, 8) growing best with ammonium chloride as nitrogen source, the results being given in the following table:
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optimizing the carbon/nitrogen source ratio of the hydrocarbon degrading bacterium nutrition activator:
for 8 strains of oil extraction functional bacteria (H65-3, 4, 6, 11, 13, 15, 18 and 20 respectively), 1.0% of corn steep liquor dry powder is used as a unique carbon source, 1% of inoculum size is used for culturing for 36 hours, and OD of the culture solution is measured 600 . According to the OD of the measurement culture solution 600 The optimal formula of the nutrition activator of the strain H65-3 is 1% corn steep liquor dry powder, 0.1% sodium nitrate and 0.2% dipotassium hydrogen phosphate; the optimal formula of the nutrition activators of the strains H65-4, 6, 11, 15, 18 and 20 is 1 percent of corn steep liquor dry powder, 0.2 percent of sodium nitrate and 0.2 percent of dipotassium hydrogen phosphate; the optimal nutrition activator formula of the strain H65-13 is 0.8% corn steep liquor dry powder, 0.2% sodium nitrate and 0.2% dipotassium hydrogen phosphate; the optimal formulation for the nutritional activator for strain H70-2 is 1% corn steep liquor dry powder +0.2% sodium nitrate +0.2% dipotassium hydrogen phosphate, the results are shown in the following table:
meanwhile, the growth experiment shows that: in addition, the optimal culture medium of the two strains H65-1 and 8 is 0.4% corn steep liquor dry powder, 0.2% sodium nitrate and 0.2% hydrogen diamine phosphate, and the carbon-nitrogen source proportion optimization results are shown in the following table:
example 3
Determination of injection quantity and concentration of microbial strain-expelling
In order to determine the optimal injection amount and injection concentration of the microbial system, a physical simulation oil displacement experiment was first performed from the development of microbial slug optimization, and an experimental model used small-size artificial cores (2.5 x 10cm, average permeability 650 mD).
The design experiment group and the control group are shown in the following table:
in the table, v represents the experimental group.
Microorganism slug optimization oil displacement model construction and basic parameters thereof are listed in the following table
The higher the concentration of the microbial system, the higher the oil displacement efficiency under the condition of the same microbial slug injection amount (PV number).
The oil increasing amplitude and the economic effect of the microorganism systems with different concentrations are combined, and the proper microorganism injection concentration is selected to be 1%.
The recovery at different slug sizes, the same microorganism system concentration is as follows:
under the condition of the same microorganism system concentration, the higher the microorganism slug injection amount (PV number), the higher the oil displacement efficiency of the injection stage, although the improvement recovery ratio of the injection stage is continuously increased along with the increase of the PV number; according to the situation that the total recovery rate value is improved by corresponding to the follow-up water flooding according to different PV numbers, 0.15PV is selected to be proper.
Microorganism-chemistry combination flooding injection slug optimization
After the reasonable injection concentration and injection quantity of the microorganism are determined, the order of the microorganism-chemical composite flooding slugs is further optimized, and the position of the microorganism slugs in the composite flooding system is determined. The experimental model used three layers of heterogeneous artificial cores (4.5 x 30cm, average permeability 650 mD). The composition, injection amount and concentration of each slug in the experiment are shown in the following table:
the experimental group and the control group of the composite driving slug sequence optimization design are shown in the following table
| Test group number | Experiment group slug design |
| 1 (original scheme-control group) | Front slug + main slug + protection slug (weak triple drive) |
| 2 (Experimental group) | Microorganism slug + leading slug + main slug + protection slug |
| 3 (Experimental group) | Front slug + microbial slug + main slug + protection slug |
| 4 (Experimental group) | Front slug + main slug + protection slug + microbial slug |
| 5 (Experimental group) | 1/2 microbial slug + pre-slug +1/2 microbial slug + main slug + protection slug |
Basic parameters of the oil displacement model of the compound oil displacement slug sequence optimization experiment are listed in the table
The water content-pressure-displacement efficiency of the displacement under different slug sequences is shown in fig. 10-15, in each of which ■ dotted line represents water content, X dotted line represents recovery, and dotted line represents pressure, wherein the result of scheme 1 (weak base ternary body displacement) is shown in fig. 11, the result of scheme 11 is shown in fig. 11, the recovery ratio is 27.92% relative to water displacement, the result of scheme 2 (microbial slugs are placed in front of the front slug) is shown in fig. 12, the result of scheme 12 is shown in fig. 12, the recovery ratio is 35.19% relative to water displacement, and the displacement effect is better than that of scheme 1; the results of scheme 3 (microbial slugs placed before the main slugs) are shown in fig. 13, as can be obtained from fig. 13, with an enhanced recovery ratio of 31.53% relative to water flooding, and the results of scheme 4 (microbial slugs placed after the protection slugs) are shown in fig. 14, as can be obtained from fig. 14, with an enhanced recovery ratio of 29.91% relative to water flooding; scheme 3 has better effect than scheme 4 and also better effect than scheme 1; the results of scheme 5 (microbial slugs placed before the main slug and the pre-slug, respectively) are shown in fig. 15, and as can be obtained from fig. 15, the recovery ratio is improved by 33.22% relative to the water flooding, and the effect is superior to that of scheme 1, scheme 3 and scheme 4.
Composite slug position optimization each experimental group enhanced recovery rate values are shown in the table below
The oil displacement effect of scheme 2 (the microbial slug is arranged in front of the pre-slug) is obviously better than that of other schemes by optimizing each experimental group by combining the positions of the composite flooding slugs, and the oil displacement efficiency is 35.19% by adopting the slug under the same PV number, and compared with scheme 1, the recovery ratio is improved by 7.27%. Scheme 4 (after the microbial slug is placed in the protection slug) has poor oil displacement effect, and the recovery ratio is only improved by 1.99% compared with the original scheme.
One or more technical solutions in the embodiments of the present application at least have the following technical effects or advantages:
(1) The embodiment of the application provides a microbial chemical compound efficient oil displacement system for improving the recovery ratio of high-freezing crude oil;
(2) The oil displacement system provided by the embodiment of the application has obvious functions of reducing the pour point and viscosity of crude oil and preventing wax precipitation;
(3) The micro-molecular biological surfactant produced by microorganism metabolism in the oil displacement system provided by the embodiment of the application can play a role in synergy with the surfactant, reduce the tension of an oil-water interface and enhance the oil washing capability;
(4) The oil displacement system provided by the embodiment of the application can greatly improve the recovery ratio of crude oil.
Finally, it is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present application have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the application.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present application without departing from the spirit or scope of the application. Thus, it is intended that the present application also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (4)
1. An oil displacement method for improving the crude oil recovery ratio of a high-freezing-point oil reservoir is characterized in that a microbial oil displacement system and a chemical oil displacement system of the oil displacement system are sequentially injected into the high-freezing-point oil reservoir for oil displacement treatment so as to improve the crude oil recovery ratio of the high-freezing-point oil reservoir;
the microbial oil displacement system comprises: the chemical oil displacement system comprises a weak base ternary system, a pre-slug liquid and a protection slug liquid;
the method specifically comprises the following steps:
s1, injecting the microorganism oil displacement system into a subsurface oil-containing layer to form a microorganism slug;
s3, injecting the pre-slug liquid into the underground oil-bearing layer to form a pre-slug;
s4, injecting the weak base ternary system into the underground oil-bearing layer to form a main slug;
s5, injecting the protection slug liquid into the underground oil-containing layer to form a protection slug;
the microbial slugs, the pre-slug, the main slug and the protection slug are sequentially injected from the surface to an oil-water mixing zone of the underground containing layer and are finally mixed in the oil-water mixing zone, and the injection volume of the microbial oil displacement system accounts for 10% -20% of the total pore volume of the underground oil-containing layer; the injection volume of the pre-slug liquid accounts for 5-15% of the total pore volume of the underground oil-bearing layer; the injection volume of the weak base ternary system accounts for 75% -85% of the total pore volume of the underground oil-bearing layer; the injection volume of the protection slug liquid accounts for 5-15% of the total pore volume of the underground oil-containing layer, and the bacterial content of the hydrocarbon degrading bacterial agent is 10 7 cfu/mL-10 8 cfu/mL；
In the microbial oil displacement system, the weight concentration of the hydrocarbon degrading bacterial agent is 4500mg/L-5500mg/L;
the chemical flooding system comprises a weak base ternary system, wherein the weak base ternary system comprises a mixture of an anionic surfactant, a polymer, sodium carbonate and water, the anionic surfactant is a petroleum sulfonate surfactant, and the polymer is first partially hydrolyzed polyacrylamide;
in the weak base ternary system, the weight concentration of the petroleum sulfonate surfactant is 500mg/L-3000mg/L, the weight concentration of the first partially hydrolyzed polyacrylamide is 1000mg/L-2000mg/L, and the weight concentration of the sodium carbonate is 500mg/L-4000mg/L;
the pre-slug liquid is second partially hydrolyzed polyacrylamide, and the weight concentration of the second partially hydrolyzed polyacrylamide is 2000-3000mg/L; the viscosity of the second partially hydrolyzed polyacrylamide is 100-200 mpa.s;
the protection slug liquid is third partially hydrolyzed polyacrylamide, and the weight concentration of the third partially hydrolyzed polyacrylamide is 800mg/L-2000mg/L; the viscosity of the third partially hydrolyzed polyacrylamide is 50-150 mpa.s.
2. The oil displacement method for improving the recovery ratio of crude oil in a high-freezing-point oil reservoir according to claim 1, wherein hydrocarbon degrading bacteria of the hydrocarbon degrading bacteria agent are obtained by carrying out bacteria enrichment and screening on oil well produced liquid of the high-freezing-point oil reservoir to be displaced, and the hydrocarbon degrading bacteria can generate biosurfactants.
3. The method of claim 1, wherein the microbial flooding system further comprises a microbial activator; the microbial activator comprises a mixture of corn steep liquor dry powder, sodium nitrate, diammonium phosphate and yeast powder.
4. The method of claim 3, wherein in the microbial oil displacement system, the weight concentration of the corn steep liquor dry powder is 500mg/L-1500mg/L; the weight concentration of the sodium nitrate is 500mg/L-1500mg/L; the weight concentration of the diammonium hydrogen phosphate is 500mg/L-1500mg/L; the weight concentration of the yeast powder is 200mg/L-300mg/L.
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| 微生物―三元复合驱提高采收率的研究;陈美竹;;内蒙古石油化工(16);第122-124页 * |
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