CN115516105A - 经由杂交链反应分析样品内的靶分子 - Google Patents

经由杂交链反应分析样品内的靶分子 Download PDF

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Publication number
CN115516105A
CN115516105A CN202180033401.5A CN202180033401A CN115516105A CN 115516105 A CN115516105 A CN 115516105A CN 202180033401 A CN202180033401 A CN 202180033401A CN 115516105 A CN115516105 A CN 115516105A
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China
Prior art keywords
hcr
initiator
probe
sample
probes
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Pending
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CN202180033401.5A
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English (en)
Chinese (zh)
Inventor
N·A·皮尔斯
H·M·T·蔡
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Merck Euler Equipment Co ltd
California Institute of Technology
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Merck Euler Equipment Co ltd
California Institute of Technology
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Application filed by Merck Euler Equipment Co ltd, California Institute of Technology filed Critical Merck Euler Equipment Co ltd
Publication of CN115516105A publication Critical patent/CN115516105A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180033401.5A 2020-03-06 2021-03-04 经由杂交链反应分析样品内的靶分子 Pending CN115516105A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062986436P 2020-03-06 2020-03-06
US62/986,436 2020-03-06
PCT/US2021/020919 WO2021221789A2 (en) 2020-03-06 2021-03-04 Analysis of target molecules within a sample via hybridization chain reaction

Publications (1)

Publication Number Publication Date
CN115516105A true CN115516105A (zh) 2022-12-23

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CN202180033401.5A Pending CN115516105A (zh) 2020-03-06 2021-03-04 经由杂交链反应分析样品内的靶分子

Country Status (9)

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US (2) US20220282300A1 (https=)
EP (2) EP4114969B1 (https=)
JP (2) JP2023517054A (https=)
KR (1) KR20220151183A (https=)
CN (1) CN115516105A (https=)
AU (1) AU2021265719A1 (https=)
CA (1) CA3173975A1 (https=)
IL (1) IL295981A (https=)
WO (1) WO2021221789A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121653230A (zh) * 2026-02-06 2026-03-13 中国科学技术大学 高通量原位检测生物样品内核酸分子的探针组、试剂盒和方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL290679B2 (en) 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
GB202100760D0 (en) * 2021-01-20 2021-03-03 Qbiotix Ltd Target-dependent polymerisation of oligonucleotides
WO2022164796A1 (en) 2021-01-26 2022-08-04 California Institute Of Technology Allosteric conditional guide rnas for cell-selective regulation of crispr/cas
EP4482982A1 (en) 2022-02-21 2025-01-01 Kanvas Biosciences, Inc Highly multiplexed detection of gene expression with hybridization chain reaction
CN115961011B (zh) * 2022-12-19 2025-04-08 中山大学 一种基于金纳米粒子和杂交链反应扩增检测甲醛的方法
CN116206684B (zh) * 2022-12-26 2024-01-30 纳昂达(南京)生物科技有限公司 一种评估基因组重复区探针捕获安全性的方法及其装置
US20240336956A1 (en) 2023-04-04 2024-10-10 California Institute Of Technology Ultrasensitive molecular detection via hybridization chain reaction
US20250011843A1 (en) * 2023-07-07 2025-01-09 California Institute Of Technology Molecular detection using barcode probes or barcode fusion probes and hybridization chain reaction
WO2025076345A1 (en) * 2023-10-06 2025-04-10 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Compositions and methods for identifying bacterial characteristics
GB202402960D0 (en) * 2024-02-29 2024-04-17 Nanovery Ltd Catalytic nucleic acid system

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098049A2 (en) 2004-03-25 2005-10-20 California Institute Of Technology Hybridization chain reaction
EP1799825B1 (en) * 2004-10-05 2011-06-29 The California Institute of Technology Aptamer regulated nucleic acids and uses thereof
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
DK2444807T3 (da) * 2006-11-01 2014-08-11 Ventana Med Syst Inc Mono- og dinitropyrazolhaptenkonjugater
US8793705B2 (en) 2007-08-31 2014-07-29 Red Hat, Inc. Rich internet bus
GB201401885D0 (en) * 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
US10364457B2 (en) 2015-08-07 2019-07-30 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
IL290679B2 (en) * 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
CA3034617A1 (en) * 2016-08-30 2018-03-08 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
US11459603B2 (en) * 2017-05-23 2022-10-04 Rutgers, The State University Of New Jersey Target mediated in situ signal amplification with dual interacting hairpin probes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121653230A (zh) * 2026-02-06 2026-03-13 中国科学技术大学 高通量原位检测生物样品内核酸分子的探针组、试剂盒和方法

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Publication number Publication date
EP4114969B1 (en) 2025-08-06
JP2026065072A (ja) 2026-04-14
EP4114969C0 (en) 2025-08-06
US20230193362A1 (en) 2023-06-22
US20220282300A1 (en) 2022-09-08
KR20220151183A (ko) 2022-11-14
WO2021221789A3 (en) 2022-03-24
EP4592389A3 (en) 2025-08-13
EP4114969A2 (en) 2023-01-11
CA3173975A1 (en) 2021-11-04
EP4114969A4 (en) 2024-03-06
IL295981A (en) 2022-10-01
EP4592389A2 (en) 2025-07-30
JP2023517054A (ja) 2023-04-21
WO2021221789A2 (en) 2021-11-04
AU2021265719A1 (en) 2022-11-03

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