CN115505509A

CN115505509A - Intestinal pathogenic bacteria sampling culture apparatus

Info

Publication number: CN115505509A
Application number: CN202211181968.XA
Authority: CN
Inventors: 殷雪瑞
Original assignee: Chengdu Shuangliu District First People's Hospital
Current assignee: Chengdu Shuangliu District First People's Hospital
Priority date: 2022-09-27
Filing date: 2022-09-27
Publication date: 2022-12-23

Abstract

The invention belongs to the field of germ culture equipment, and particularly relates to a sampling and culturing device for intestinal pathogenic bacteria. The specific technical scheme is as follows: including box, the case lid that can dismantle the connection, circular array is provided with a plurality of culture dishes in the box, the case lid is including rotating first apron, the second apron that sets up, seted up respectively on first apron, the second apron with corresponding first sample through-hole, the second sample through-hole of culture dish, be provided with the dwang in the box, the dwang passes first apron and first apron fixed connection, and passes the second apron and rotate with the second apron and be connected. Can be used to cultivate different kinds of intestinal pathogenic bacteria in every culture dish, except treating the culture dish of sample, the arc sealing head continues to seal other culture dishes, prevents that other culture dishes from being polluted by the external world.

Description

Intestinal pathogenic bacteria sampling culture apparatus

Technical Field

The invention belongs to the field of germ culture equipment, and particularly relates to a sampling and culturing device for intestinal pathogenic bacteria.

Background

The intestinal pathogenic bacteria refer to bacteria which can cause intestinal diseases and the like besides normal large intestine flora, and mainly comprise salmonella, shigella, proteus, escherichia coli, yersinia, and the like. When pathogenic bacteria enter a human body and multiply, a series of intestinal reactions, such as diarrhea, stomachache and the like, can be caused. However, the intestinal pathogenic bacteria causing diarrhea are various, and the content of the intestinal pathogenic bacteria in feces is usually low, so that the intestinal pathogenic bacteria in feces need to be cultured in an enrichment manner in clinic, and then the root cause of the intestinal disease can be found through multiple tests, so that the detection rate of the intestinal pathogenic bacteria is improved.

When the intestinal colony is cultured clinically, physiological saline and fresh excrement are mixed according to a certain proportion to prepare turbid liquid, the turbid liquid is mixed with anaerobic culture solution according to a certain proportion, and finally the turbid liquid is mixed with medicines according to a certain proportion and then is subjected to anaerobic culture at a certain temperature, and sampling detection is carried out after the culture is finished. As the intestinal pathogenic bacteria are various, different culture mediums are needed to be adopted to culture the turbid liquid. After the culture is finished, the culture dish needs to be opened every time sampling detection is carried out. The existing culture dish only adopts a simple cover, and after the culture dish is opened, air and bacteria in the environment enter the culture dish to pollute culture solution in the culture dish and influence subsequent detection results.

Aiming at the technical problems, if the intestinal pathogenic bacteria culture device which can culture various pathogenic bacteria at one time and has no pollution or less pollution during sampling can be provided, the intestinal pathogenic bacteria culture device has excellent medical application prospect.

Disclosure of Invention

In order to solve the technical problems in the background art, the invention provides a sampling and culturing device for intestinal pathogenic bacteria.

In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: the utility model provides an intestinal pathogenic bacteria sampling culture apparatus, is including dismantling box, case lid of connection, circular array is provided with a plurality of culture dishes in the box, the case lid is including rotating first apron, the second apron that sets up, first apron, second apron on seted up respectively with corresponding first sample through-hole, the second sample through-hole of culture dish, be provided with the dwang in the box, the dwang passes first apron and first apron fixed connection, and passes the second apron and rotate with the second apron and be connected.

Preferably: first apron setting is in second apron below, first apron lower extreme be provided with a plurality ofly with the sealed head of the corresponding arc of culture dish, the dwang goes up and down to set up in the box, when the dwang reciprocated, drive first apron, second apron and reciprocate.

Preferably: be provided with the third stopper on the dwang lower extreme lateral wall, the bottom is provided with the dead lever in the box, the dead lever upper end seted up with the first recess that the dwang corresponds, from top to bottom the interval be provided with in the first recess with second recess, the third recess that the dwang lower extreme corresponds, the regional between first recess inside wall, second recess and the third recess seted up with the corresponding breach of third stopper.

Preferably: the lower end of the rotating rod is provided with an accommodating groove, the third limiting block is rotatably arranged in the accommodating groove through a torsion spring, and the accommodating groove accommodates the third limiting block; and in a natural state, the third limiting block is vertical to the accommodating groove.

Preferably: the box cover is provided with a positioning mechanism, the positioning mechanism comprises a first limiting groove arranged on the upper surface of the first cover plate, the lower surface of the second cover plate is provided with a first positioning groove, a first spring is arranged in the first positioning groove, and the other end of the first spring is provided with an arc-shaped first limiting block corresponding to the first limiting groove.

Preferably, the following components: the box cover is provided with a positioning mechanism, the positioning mechanism comprises a first limiting groove arranged on the lower surface of the second cover plate, the upper surface of the first cover plate is provided with a first positioning groove, a first spring is arranged in the first positioning groove, and the other end of the first spring is provided with an arc-shaped first limiting block corresponding to the first limiting groove.

Preferably, the following components: still be provided with fixed establishment on the case lid, fixed establishment includes last the last fixed orifices who offers of second apron, with the fixed block that fixed orifices corresponds, first apron upper surface is provided with the fixed slot, transversely set up the second spring in the fixed slot, the second spring other end is provided with trapezoidal extrusion piece, the fixed block lower extreme with the contact surface of trapezoidal extrusion piece is the wedge.

Preferably, the following components: the fixed block lower extreme is the quadrangular frustum pyramid of invering, first apron upper surface is provided with four the fixed slot, each set up the second spring in the fixed slot, the second spring other end is provided with trapezoidal extrusion piece.

Preferably, the following components: the case lid still includes the case lid casing, the vertical spout that is provided with on the case lid casing, first apron periphery is provided with the connecting block, the connecting block inboard is provided with the fourth recess, first apron periphery rotates and sets up in the fourth recess, the connecting block is kept away from first apron one side and is slided and set up on the spout.

Correspondingly: a using method of a sampling and culturing device for intestinal pathogenic bacteria comprises the following steps,

s1, lifting the rotating rod upwards to a third limiting block to be located in the second groove, and rotating the first cover plate until the first sampling through hole is aligned with a culture dish to be sampled;

s2, rotating the second cover plate until the first sampling through hole is aligned with the second sampling through hole;

s3, pressing the fixing block downwards, pressing the rotating rod downwards until the first sampling through hole and the second sampling through hole are aligned with the corresponding culture dish, and starting sampling;

s4, after sampling is finished, lifting the fixing block upwards, and rotating the second cover plate until the first sampling through hole and the second sampling through hole are staggered;

s5, lifting the rotating rod upwards until the third limiting block is located in the second groove, and rotating the rotating rod until the third limiting block is aligned with the notch;

s6, the rotating rod is pressed downwards, the first sampling through hole is aligned to the blank position, and the second cover plate is rotated to be staggered with the first sampling through hole and the second sampling through hole.

The invention has the following beneficial effects: set up first apron through the activity on the case lid, the second apron, set up first sample through-hole respectively on it, second sample through-hole, first sample through-hole, second sample through-hole can misplace, can align, circular array has a plurality of culture dishes in the box, first apron lower surface is provided with a plurality of arc sealed heads corresponding with the culture dish, can be used to cultivate different types of intestinal pathogenic bacteria in every culture dish, except treating the culture dish of sample, arc sealed head continues to seal other culture dishes, prevent that other culture dishes from being contaminated by the external world.

Drawings

FIG. 1 is a schematic view of the overall structure of the sampling and culturing apparatus according to the present invention (in a sealed state);

FIG. 2 is a schematic view of a portion A of FIG. 1;

FIG. 3 is a schematic view of a portion B of FIG. 1;

fig. 4 is a schematic sectional view (three states) of the connection between the rotating rod and the fixed rod according to the present invention;

FIG. 5 is a schematic three-dimensional perspective view of a fixation rod of the present invention;

FIG. 6 is a schematic top sectional view of the container of the present invention;

FIG. 7 is a schematic view of the entire structure of the sampling culture apparatus of the present invention (non-sealed state).

In the figure: the box 1, the second apron 2, first apron 3, culture dish 4, sealed head 5, spout 6, connecting block 7, handle 8, dwang 9, dead lever 10, fixed block 11, fixing hole 12, slider 13, case lid casing 14, fourth recess 15, first recess 16, third recess 17, second recess 18, breach 19, holding tank 20, third stopper 21, blank position 22, first spring 23, first locating groove 24, first stopper 25, first spacing groove 26, second spring 27, fixed slot 28, trapezoidal extrusion piece 29.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1-7, an intestinal pathogenic bacteria sampling and culturing device comprises a box body 1 and a box cover which are detachably connected, wherein the box body 1 and the box cover can be connected in a fastening, inserting, bolt connection mode, and the like, and a sealing ring can be arranged at the connecting part of the box body 1 and the box cover to ensure the sealing performance of the culturing device. Circle array is provided with a plurality of culture dishes 4 in 1 box, can be used to cultivate different types of enteropathogenic bacteria in every culture dish 4, and the different culture medium that corresponds enteropathogenic bacteria is placed to every culture dish 4 promptly. The case lid is including rotating first apron 3, the second apron 2 that sets up, first apron 3, second apron 2 go up seted up respectively with 4 corresponding first sample through-holes, the second sample through-holes of culture dish, the internal dwang 9 that is provided with of box, dwang 9 passes first apron 3 and first apron 3 fixed connection, and passes second apron 2 and rotate and be connected, and the second apron 2 external connection that stretches out is lapped to dwang 9 upper end has handle 8. The first cover plate 3 and the second cover plate 2 can rotate together, the second cover plate 2 can also rotate relative to the first cover plate 3, and the first cover plate 3 can also rotate relative to the second cover plate 2. According to the preferable technical scheme, a position of the culture dish 4 is reserved in the box body 1, the position is a blank position 22, namely the blank position 22 does not actually place the culture dish 4, and after sampling is completed or in a culture period, the first sampling through hole is aligned to the blank position 22.

As shown in fig. 1 and 7, during sampling, the second cover plate 2 is rotated, the first cover plate 3 is kept stationary, the second sampling through hole is aligned with the first sampling through hole, then the first cover plate 3 and the second cover plate 2 are rotated simultaneously, the first sampling through hole and the second sampling through hole are aligned with the culture dish 4, and the culture solution in the corresponding culture dish 4 is sampled and detected. After the sampling is finished, the first cover plate 3 and the second cover plate 2 are rotated simultaneously to enable the first sampling through hole and the second sampling through hole to be aligned to the blank position 22, and then only the second cover plate 2 is rotated, the first cover plate 3 is kept still, and the first sampling through hole and the second sampling through hole are staggered.

Or, during sampling, the first cover plate 3 is rotated firstly, the second cover plate 2 is kept still, the first sampling through hole is aligned to the culture dish 4 needing sampling, then the second cover plate 2 is rotated, the second sampling through hole is aligned to the first sampling through hole, and the culture solution in the corresponding culture dish 4 is sampled and detected. After the sampling is finished, the first cover plate 3 and the second cover plate 2 are rotated simultaneously, so that the first sampling through hole is aligned to the blank position 22, and then only the second cover plate 2 is rotated, so that the first cover plate 3 is kept still, and the first sampling through hole and the second sampling through hole are staggered.

Further, the first cover plate 3 is arranged below the second cover plate 2, a plurality of arc-shaped sealing heads 5 corresponding to the culture dish 4 are arranged at the lower end of the first cover plate 3, and the arc-shaped sealing heads 5 protrude out of the first cover plate 3. The arc-shaped sealing head 5 is made of elastic materials. Dwang 9 goes up and down to set up in the box 1, when dwang 9 reciprocated, drive first apron 3, second apron 2 and reciprocate. During the cultivation period, the blank position 22 is aimed at to first sample through-hole, and the opening part of all culture dishes 4 in the box 1 all corresponds there is the sealed head 5 of arc, seals culture dish 4. During the sample, dwang 9 rebound, arc seal head 5 break away from culture dish 4, and when rotating first apron 3, aim at corresponding culture dish 4 with first sample through-hole, move dwang 9 rebound again, arc seal head 5 continues to seal other culture dishes 4, prevents that other culture dishes 4 from being polluted by the external world. Although the culture dishes 4 use different arc-shaped sealing heads 5 during sampling, the excrement suspension in all the culture dishes 4 is the same, the types of the intestinal pathogens are the same, and the detection aims at detecting all the intestinal pathogens, so that the problem of cross contamination of the intestinal pathogens among the culture dishes 4 does not exist. After the sample is accomplished, dwang 9 rebound, arc seal head 5 break away from culture dish 4, and when rotating first apron 3, with the blank position 22 of first sample through-hole alignment, again with dwang 9 rebound, arc seal head 5 seals culture dish 4.

As shown in FIGS. 4 and 5, (a) in FIG. 4 shows a state where the rotating lever is pushed down to the bottom of the first well during sampling, (b) shows a state during the incubation period, and (c) shows a state where the rotating lever is pulled up into the second well during sampling. Be provided with third stopper 21 on the lateral wall of dwang 9 lower extreme, the bottom is provided with dead lever 10 in box 1, dead lever 10 upper end seted up with the first recess 16 that dwang 9 corresponds. Housing groove 20 has been seted up on the lateral wall under dwang 9, and third stopper 21 passes through the torsional spring and rotates the setting in housing groove 20, and housing groove 20 can hold third stopper 21. By arranging the torsion spring, in a natural state, the third limiting block 21 is horizontally arranged relative to the rotating rod 9, namely, the length direction of the rotating rod 9 is vertical to the length direction of the third limiting block 21; when an external force is applied, the third stopper 21 is located in the receiving groove 20. A second groove 18 and a third groove 17 corresponding to the lower end of the rotating rod 9 are arranged in the first groove 16 from top to bottom at intervals. A gap 19 corresponding to the third limiting block 21 is formed in the inner side wall of the first groove 16, the area between the second groove 18 and the third groove 17, the size of the gap 19 corresponds to the size of the third limiting block 21, namely, when the third limiting block 21 is in a natural state, the gap 19 can allow the third limiting block 21 to pass through. The position of the notch 19 corresponds to the position of the blank position 22, that is, the center of the blank position 22 falls on the vertical plane of the center line of the notch 19 in the horizontal direction, and the notch 19 and the blank position 22 are located on the same side of the rotating rod 9. Preferably, in the culturing period, the center of the first sampling through hole, the center line of the third limiting block 21 in the horizontal direction, and the center line of the notch 19 in the horizontal direction are located on the same plane.

It should be noted that the radius of the rotating rod 9 is R1, and the distance from the outermost periphery of the third limiting block 21 to the central axis of the rotating rod 9 is R2. The inner diameter of the first groove 16 corresponds to the radius R1 of the rotating lever 9, i.e. the inner diameter of the first groove 16 is slightly larger than the radius R1 of the rotating lever 9, so that the upper end of the rotating lever 9 can slide up and down in the first groove 16. That is, the inner diameters of the second groove 18 and the third groove 17 correspond to R2, so that the lower end of the rotating lever 9 can slide up and down in the second groove 18 and the third groove 17.

During the culture period, the lower end of the rotating rod 9 is positioned in the third groove 17. During sampling, the rotating rod 9 is rotated to the third limiting block 21 to be aligned with the notch 19, the rotating rod 9 is pulled upwards, the rotating rod 9 enters the second groove 18 from the third groove 17, the lower end of the rotating rod 9 rotates in the second groove 18, and the third limiting block 21 is in a horizontal state; rotatory dwang 9 aligns with corresponding culture dish 4 to first sample through-hole, pushes down dwang 9, and third stopper 21 falls into holding tank 20 in, and third stopper 21 does not fall into third recess 17 this moment. After the sample was accomplished, upward draw dwang 9, until third stopper 21 falls into second recess 18 in, rotatory dwang 9 aims at with breach 19 to third stopper 21, pushes down dwang 9, dwang 9 falls into third recess 17 from second recess 18 in.

Further technical scheme, when rotating second apron 2, in order to aim at first sample through-hole and second sample through-hole fast, be provided with positioning mechanism on the case lid. One embodiment of the positioning mechanism, as shown in fig. 2, includes a first limiting groove 26 disposed on the upper surface of the first cover plate 3, a first positioning groove 24 is disposed on the lower surface of the second cover plate 2, a first spring 23 in a compressed state is disposed in the first positioning groove 24, and an arc-shaped first limiting block 25 corresponding to the first limiting groove 26 is disposed at the other end of the first spring 23. Another embodiment of the positioning mechanism includes a first limiting groove 26 disposed on the lower surface of the second cover plate 2, a first positioning groove 24 is disposed on the upper surface of the first cover plate 3, a first spring 23 in a compressed state is disposed in the first positioning groove 24, and an arc-shaped first limiting block 25 corresponding to the first limiting groove 26 is disposed at the other end of the first spring 23. During positioning, the first limiting block 25 falls into the first limiting groove 26, and the first sampling through hole is aligned with the second sampling through hole.

In a further technical scheme, as shown in fig. 3, a fixing mechanism is further arranged on the box cover, the fixing mechanism includes a fixing through hole 12 formed in the second cover plate 2 and a fixing block 11 corresponding to the fixing through hole 12, a fixing groove 28 is arranged on the upper surface of the first cover plate 3, and the lower end of the fixing block 11 penetrates through the fixing through hole 12 and can extend into the fixing groove 28. A second spring 27 in a compressed state is transversely arranged in the fixing groove 28, a trapezoidal extrusion block 29 is arranged at the other end of the second spring 27, and the contact surface between the lower end of the fixing block 11 and the trapezoidal extrusion block 29 is a wedge-shaped surface. When the second cover plate 2 rotates along with the first cover plate 3, the lower end of the fixing block 11 is inserted into the fixing groove 28, and the trapezoid extrusion block 29 is tightly abutted to the lower end of the fixing block 11. When only the second cover plate 2 is rotated, the fixing block 11 is lifted upward, so that the lower end of the fixing block 11 is separated from the fixing groove 28.

In a preferred technical scheme, the lower end of the fixing block 11 is an inverted quadrangular frustum pyramid, four fixing grooves 28 are formed in the upper surface of the first cover plate 3, a second spring 27 in a compressed state is arranged in each fixing groove 28, and a trapezoidal extrusion block 29 is arranged at the other end of each second spring 27.

Further, the case lid still includes case lid casing 14, the vertical spout 6 that is provided with on the case lid casing 14, first apron 3 periphery is provided with connecting block 7, and connecting block 7 slides and sets up on the 14 inner walls of case lid casing, connecting block 7 inboard is provided with the fourth recess 15 of "[" form, first apron 3 periphery is rotated and is set up in fourth recess 15, one side of keeping away from first apron 3 on connecting block 7 is provided with slider 13, slider 13 slides and sets up on the spout 6. When the first cover plate 3 moves up and down, the connecting block 7 slides up and down on the box cover shell 14, and the sliding block 13 slides in the sliding groove 6. Note that the periphery of the second cover 2 can slide up and down and rotate on the inner wall of the cover case 14.

During the culture period, the first sampling through hole is aligned with the blank position 22, and the first sampling through hole and the second sampling through hole are staggered. When sampling, the use method of the intestinal pathogenic bacteria sampling and culturing device comprises the following steps:

s1, pulling the rotating rod 9 upwards, enabling the lower end of the rotating rod 9 to enter the second groove 18 from the third groove 17, rotating the rotating rod 9, enabling the lower end of the rotating rod 9 to rotate in the second groove 18, and driving the first cover plate 3 to rotate until the first sampling through hole is aligned with the culture dish 4 to be sampled;

s2, rotating the second cover plate 2 to align the first sampling through hole with the second sampling through hole;

s3, downwards pressing the fixing block 11, downwards pressing the rotating rod 9, aligning the first sampling through hole and the second sampling through hole with the corresponding culture dishes 4, starting sampling, and sealing the rest culture dishes 4 by the arc-shaped sealing heads 5 in the sampling process;

s4, after sampling is finished, lifting the fixing block 11 upwards, rotating the second cover plate 2 until the first sampling through hole and the second sampling through hole are staggered, and ensuring that the culture dish 4 is not polluted by the outside;

s5, the rotating rod 9 is pulled upwards, the lower end of the rotating rod 9 rotates in the second groove 18, and the rotating rod 9 is rotated until the third limiting block 21 is aligned with the notch 19;

s6, push down dwang 9, make the sealed head of arc 5 seal up culture dish 4, blank position 22 is aimed at to first sample through-hole, rotates second apron 2 simultaneously to first sample through-hole, second sample through-hole dislocation.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes, modifications, alterations, and substitutions which may be made by those skilled in the art without departing from the spirit of the present invention shall fall within the protection scope defined by the claims of the present invention.

Claims

1. The utility model provides an intestinal pathogenic bacteria sampling culture apparatus which characterized in that: including box (1), the case lid of dismantling the connection, circle shape array is provided with a plurality of culture dishes (4) in box (1), the case lid is including rotating first apron (3), second apron (2) that set up, seted up respectively on first apron (3), second apron (2) with corresponding first sample through-hole, the second sample through-hole of culture dish (4), be provided with dwang (9) in box (1), dwang (9) pass first apron (3) and first apron (3) fixed connection, and pass second apron (2) and rotate and be connected.

2. The intestinal pathogenic bacteria sampling and culturing device according to claim 1, characterized in that: first apron (3) set up second apron (2) below, first apron (3) lower extreme be provided with a plurality of with sealed head (5) of corresponding arc of culture dish (4), dwang (9) go up and down to set up in box (1), when dwang (9) reciprocated, drive first apron (3), second apron (2) and reciprocate.

3. The intestinal pathogenic bacteria sampling and culturing device as claimed in claim 2, wherein: be provided with third stopper (21) on dwang (9) lower extreme lateral wall, the bottom is provided with dead lever (10) in box (1), dead lever (10) upper end seted up with first recess (16) that dwang (9) correspond, from top to bottom the interval be provided with in first recess (16) with second recess (18), third recess (17) that dwang (9) lower extreme corresponds, first recess (16) inside wall, second recess (18) and third recess (17) between the region seted up with corresponding breach (19) of third stopper (21).

4. The enteropathogenic bacteria sampling and culturing device according to claim 3, wherein: an accommodating groove (20) is formed in the lower end of the rotating rod (9), and the third limiting block (21) is rotatably arranged in the accommodating groove (20) through a torsion spring; and in a natural state, the third limiting block (21) is vertical to the accommodating groove (20).

5. The intestinal pathogenic bacteria sampling and culturing device as claimed in claim 4, wherein: the box cover is provided with a positioning mechanism, the positioning mechanism comprises a first limiting groove (26) arranged on the upper surface of the first cover plate (3), the lower surface of the second cover plate (3) is provided with a first positioning groove (24), a first spring (23) is arranged in the first positioning groove (24), and the other end of the first spring (23) is provided with an arc-shaped first limiting block (25) corresponding to the first limiting groove (26).

6. The enteropathogenic bacteria sampling and culturing device according to claim 4, wherein: the box cover is provided with a positioning mechanism, the positioning mechanism comprises a first limiting groove (26) arranged on the lower surface of the second cover plate (2), the upper surface of the first cover plate (3) is provided with a first positioning groove (24), a first spring (23) is arranged in the first positioning groove (24), and the other end of the first spring (23) is provided with an arc-shaped first limiting block (25) corresponding to the first limiting groove (26).

7. An enteropathogenic bacteria sampling and culturing device according to claim 5 or 6, characterized in that: still be provided with fixed establishment on the case lid, fixed establishment includes fixed through hole (12) seted up on second apron (2), with fixed block (11) that fixed through hole (12) correspond, first apron (3) upper surface is provided with fixed slot (28), transversely set up second spring (27) in fixed slot (28), second spring (27) other end is provided with trapezoidal extrusion piece (29), fixed block (11) lower extreme with the contact surface of trapezoidal extrusion piece (29) is the wedge.

8. The intestinal pathogenic bacteria sampling and culturing device as claimed in claim 7, wherein: the lower end of the fixing block (11) is an inverted quadrangular frustum pyramid, four fixing grooves (28) are formed in the upper surface of the first cover plate (3), each fixing groove (28) is internally provided with a second spring (27), and the other end of each second spring (27) is provided with a trapezoidal extrusion block (29).

9. The enteropathogenic bacteria sampling and culturing device according to claim 2, characterized in that: the case lid still includes case lid casing (14), vertical spout (6) that are provided with on case lid casing (14), first apron (3) periphery is provided with connecting block (7), connecting block (7) inboard is provided with fourth recess (15), first apron (2) periphery is rotated and is set up in fourth recess (15), first apron (3) one side slip setting is kept away from in connecting block (7) is in on spout (6).

10. The use method of the intestinal pathogenic bacteria sampling and culturing device is characterized in that: comprises the following steps of (a) preparing a solution,

s1, upwards pulling a rotating rod (9) to a third limiting block (21) to be positioned in a second groove (18), and rotating a first cover plate (3) until a first sampling through hole is aligned with a culture dish (4) to be sampled;

s2, rotating the second cover plate (2) until the first sampling through hole is aligned with the second sampling through hole;

s3, the fixing block (11) is pressed downwards, the rotating rod (9) is pressed downwards until the first sampling through hole and the second sampling through hole are aligned with the corresponding culture dish (4), and sampling is started;

s4, after sampling is finished, the fixing block (11) is lifted upwards, and the second cover plate (3) is rotated until the first sampling through hole and the second sampling through hole are staggered;

s5, lifting the rotating rod (9) upwards to a third limiting block (21) to be located in the second groove (18), and rotating the rotating rod (9) to the third limiting block (21) to align to the notch (19);

s6, the rotating rod (9) is pressed downwards, the first sampling through hole is aligned to the blank position (22), and meanwhile the second cover plate (2) is rotated to be staggered with the first sampling through hole and the second sampling through hole.