CN115500304B

CN115500304B - Separation method of hucho taimen and fierce water fleas

Info

Publication number: CN115500304B
Application number: CN202211211509.1A
Authority: CN
Inventors: 张东; 刘鑫; 申丰源; 林听听; 李思平
Original assignee: Shandong Qianyuan Biotechnology Co ltd; East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Current assignee: Shandong Qianyuan Biotechnology Co ltd; East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Priority date: 2022-09-30
Filing date: 2022-09-30
Publication date: 2023-08-22
Anticipated expiration: 2042-09-30
Also published as: CN115500304A

Abstract

The application relates to a method for separating hucho fleas from fierce fleas. The method comprises the following steps: A. putting the collected copepods into a separation barrel, filling the barrel with water, and stopping inflating; B. illumination is arranged above the separation barrel; C. standing, discharging part of water, collecting and checking the flea proportion of the philosophy water, then adding water, repeating the process, and if the flea proportion in the collected copepods is below 5%, discarding; D. if the proportion of the hucho fleas in the collected copepods is higher than 10%, putting the collected copepods into another separating barrel, and repeating C, D until the proportion of the fleas in the discharged copepods is lower than 5%; F. finally, the copepods in the separation barrel are inspected, and when the proportion of the fleas in the water is lower than 5 percent, the copepods can be fully collected. The separation method can make the ratio of the hucho fleas in the separated copepods reach more than 95%, and the separated copepods can be safely fed to the sea horse.

Description

Separation method of hucho taimen and fierce water fleas

Technical Field

The application belongs to the field of sea horse cultivation, and particularly relates to a separation method of hucho fleas and fierce water fleas.

Background

Hippocampus is a traditional rare Chinese medicine. It is counted that the dry product of China is more than 300 tons every year. Since natural resources in China are nearly exhausted, most of sea horses in the market at present are imported, the price is increased year by year, the average price exceeds 8000 yuan per kilogram, and the value exceeds 40 hundred million yuan according to the consumption of 500 tons per year. Because of the serious damage to the natural resources of the hippocampus, artificial culture research is being carried out in many countries around the world for recovering the resources. In recent five years, the technology of cultivating the Hippocampus with the gray Hippocampus (Hippocampus erectus) as the dominant variety in China has been rapidly developed, and the cultivation of the Hippocampus has been a trend to replace the wild.

Different from general aquaculture, the sea horse cultivation is totally fed with natural fresh and alive baits, such as copepods, furosema and adult artemia, and the sea horse cultivation is similar to the baits of the sea horse in the natural environment. The sea horse mainly depends on copepods before 5cm, and although artemia larvae can be partially replaced, the growth and survival effect of sea horse seedlings are poor, and the copepods determine success or failure of sea horse cultivation to a certain extent, especially for gray sea horses. The sea horse is loved to eat the hucho fleas, does not eat the fierce water fleas, and the fierce water fleas are harmful to the sea horse, and the new-born seedlings are put into the fierce water fleas and die in 20 minutes at maximum. The radial fleas needed for cultivation are basically supplied by ponds, however, in a certain stage, the pond fierces are greatly propagated, so that the harvested radial fleas cannot be fed to the sea horse due to the fact that the ratio of fierces is too high, and the ratio of fierces to philosophy fleas reaches a level of 1:1, and even exceeds the ratio of philosophy fleas in severe cases. If the two copepods can be effectively separated, the method has important value for sea horse cultivation.

The ecological habit of the copepods is firstly mastered in the productive separation of copepods. The ecological habit of copepods is very complex, the distribution in a water body is closely related to light, some species have phototropism, phototropism and photophobicity are related to light intensity, namely the phototropism or photophobicity behavior is shown under a certain light intensity. The hucho is a planktonic species, day and night changes are distributed in the water body, and most of the fierce water fleas are benthic species and distributed in the middle and lower layers of the water body, so that a basic theoretical basis is provided for separating two types of copepods. However, the taimen is a planktonic species, but the phototactic difference is very large. In natural water areas, some species are sunk to the lower layer in the water body in the daytime, and rise to the upper layer in the evening; some species are insensitive to light intensity and only live in the upper layer; some water bodies on the upper, middle and lower layers are distributed. The hucho fleas and the fierce fleas are mixed and distributed in the pond, which shows that the fierce fleas also have certain phototactic property. In short, copepods vary widely in their response to light. At present, little research is done in this respect, especially the copepods' tendency to different light highlights and avoidance is only seen in a few species such as chinese hucho.

Disclosure of Invention

The application aims to provide a separation method of hucho fleas and fierce fleas so as to fill the blank of the prior art.

The application provides a separation method of hucho fleas and fierce fleas, which comprises the following steps:

A. putting the collected copepods into a separation barrel, filling the barrel with water, and stopping inflating;

B. the light intensity is 1000-1100lx;

C. standing, discharging part of water, collecting and checking the flea proportion of the philosophy water, then adding water, repeating the process, and if the flea proportion in the collected copepods is below 5%, discarding;

D. if the proportion of the hucho fleas in the collected copepods is higher than 10%, putting the collected copepods into another separating barrel, and repeating C, D until the proportion of the fleas in the discharged copepods is lower than 5%;

F. finally, the copepods in the separation barrel are inspected, and when the proportion of the fleas in the water is lower than 5 percent, the copepods can be fully collected.

Preferably, in the step A, the separating barrel is a conical bottom barrel similar to a artemia hatching barrel, the diameter is 85-90 cm, the barrel depth is 75-85 cm, the cone bottom is provided with a valve, the inner cone bottom and the lower part of the barrel body are 10-15 cm white, the rest are black, and the outside of the barrel is black.

Preferably, in the step B, an LED illuminating lamp is arranged at the top of the separation barrel, and the light intensity is 1000-1100lx.

Preferably, the standing time in the step C is 8-10 minutes.

Preferably, the water discharged in the step C is: the valve at the bottom of the barrel is opened to discharge water of 20-25 cm.

Preferably, the hucho fleas are spindle fleas and the fleas are tiger spot fleas.

Preferably, more than 95% of the copepods collected in the step F are fleas and are used for feeding the sea horses.

The light intensity related by the application can enable the hucho fleas to have phototaxis and simultaneously enable the fleas to generate light-shielding reaction.

Advantageous effects

The inventors have found that fleas prefer to attach to the walls of the container, especially on white walls. The application has the biggest characteristics that the fleas have certain light-avoiding property, benthonic property and white like, but the fleas have the light-tending property under certain light intensity, and the fleas which cannot feed the sea horse are separated through the designed separating barrel, so that the proportion of the fleas in the separated copepods reaches more than 95 percent, the fleas are reduced from 50 percent to less than 5 percent, the sea horse can be fed safely, and the damage to sea horse seedlings is avoided.

Detailed Description

The application will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present application and are not intended to limit the scope of the present application. Furthermore, it should be understood that various changes and modifications can be made by one skilled in the art after reading the teachings of the present application, and such equivalents are intended to fall within the scope of the application as defined in the appended claims.

Example 1

1. Separating barrel

The separating barrel is changed from a artemia hatching barrel, the volume of the barrel is about 420 liters, the depth of the barrel is 85cm, the bottom and the lower part of the inner wall of the barrel are brushed with white paint by 10 cm, the rest is black, and the outside of the barrel is black; an illuminating lamp is arranged above the separating barrel.

2. Separation process

The separation barrel is placed in a weak light environment, and the light intensity is lower than 100lx. Putting the collected copepods (weight after water filtering) into a separation barrel, and filling seawater without aeration; comparing the light intensity of four water surfaces: 600-700lx, 1000-1100lx, 1400-1500lx, 1600-1700lx; standing for 8-10 min, completely opening a valve at the bottom of the barrel to discharge water of 20 cm, collecting and checking the water flea proportion, then adding water, and repeating the process; if the collected philosophy flea proportion in the copepods is lower than 5%, discarding the philosophy flea; if the proportion of the hucho fleas in the collected copepods is higher than 10%, putting the collected copepods into another separating barrel, and repeating the process until the proportion of the fleas in the discharged copepods is lower than 5%; finally, checking the copepods in the separating barrel, and feeding the copepods in the collecting barrel with the proportion of the fierce water fleas lower than 5 percent. The four light intensity experiment effects show that the separation times of the 1000-1100lx group are the least, the fleas can be separated into 4% only by 3 times, and the 600-700lx group and the 1400-1500lx group respectively need 4 times and 5 times to reach 5%, and under 1600-1700lx light intensity, the fleas sink to the lower part and are mixed together to be difficult to separate due to the photophobic behavior of the fleas. The feeding test proves that the young sea horse cannot be harmed as long as the proportion of the fleas in the copepods is less than 5 percent. Wherein the hucho fleas are spindle fleas and the fierce fleas are tiger spot fierce fleas.

Comparative example 1

According to example 1, the inside of the separation barrel is completely black, the light intensity is 1000-1100lx, and the rest is the same as that of example 1, and the fleas can be separated to 5% only by 5 times of separation.

Comparative example 2

According to example 1, the inside of the separation barrel is full white, the light intensity is 1000-1100lx, and the rest is the same as that of example 1, and the fleas can be separated to 5% only by 6 times of separation.

While the embodiments of the present application have been described in detail, the present application is not limited to the embodiments, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the present application, and these equivalent modifications and substitutions are intended to be included in the scope of the present application as defined in the appended claims.

Claims

1. A method for separating hucho fleas from fierce fleas, comprising the following steps:

A. putting the collected copepods into a separation barrel, filling the barrel with water, stopping inflating, wherein the separation barrel is a conical bottom barrel with the diameter of 85-90 cm, the barrel depth of 75-85 cm, a cone bottom is provided with a valve, the inner cone bottom and the lower part of the barrel body are 10-15 cm white, the rest are black, and the outside of the barrel is black;

B. the light intensity is 1000-1100lx;

2. The separation method according to claim 1, wherein the illumination in step B uses an LED lamp as a light source.

3. The separation method according to claim 1, wherein the standing time in the step C is 8 to 10 minutes.

4. The separation method according to claim 1, wherein the water discharged in the step C is: the valve at the bottom of the barrel is completely opened to discharge water of 20-25 cm.

5. The method of claim 1, wherein the hucho fleas are spindle fleas and the fleas are tiger spot fleas.

6. The method of claim 1, wherein more than 95% of all copepods collected in step F are fleas for feeding the hippocampus.