CN115500304A

CN115500304A - Separation method of philosophila and daphnia magna

Info

Publication number: CN115500304A
Application number: CN202211211509.1A
Authority: CN
Inventors: 张东; 刘鑫; 申丰源; 林听听; 李思平
Original assignee: Shandong Qianyuan Biotechnology Co ltd; East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Current assignee: Shandong Qianyuan Biotechnology Co ltd; East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Priority date: 2022-09-30
Filing date: 2022-09-30
Publication date: 2022-12-23
Anticipated expiration: 2042-09-30
Also published as: CN115500304B

Abstract

The invention relates to a method for separating philosophila and daphnia magna. The method comprises the following steps: A. placing the collected copepods into a separation barrel, filling the barrel with water, and stopping inflating; B. the upper part of the separation barrel is provided with illumination; C. standing, discharging part of water, collecting and checking the proportion of philosophy water fleas, then adding water, repeating the process, and abandoning the collected philosophy water fleas if the proportion of the collected philosophy water fleas in the copepods is below 5%; D. if the proportion of collected copepods is higher than 10%, placing the collected copepods into another separation barrel, and repeating the processes C and D until the proportion of the discharged copepods is lower than 5%; F. and finally, checking copepods in the separation barrel, and completely collecting the copepods when the proportion of the harpacticoida is less than 5%. The separation method can make the separated calachis water flea proportion reach more than 95%, and can be safely fed to the hippocampus.

Description

Separation method of philosophila and daphnia magna

Technical Field

The invention belongs to the field of sea horse cultivation, and particularly relates to a method for separating philosophila and daphnia magna.

Background

The hippocampus is a traditional rare Chinese medicine. According to statistics, the amount of dry medicine used in China is more than 300 tons every year. As natural resources in China are almost exhausted, most of hippocampus required by the current market is imported, the price is increased year by year, the average price exceeds 8000 yuan per kilogram, and the value exceeds 40 hundred million yuan according to 500 tons of annual consumption. Because natural resources of hippocampus are seriously damaged, artificial breeding research is carried out in many countries in the world for recovering resources. In the last five years, the Hippocampus culture technology taking Hippocampus erectus as a leading variety in China has rapidly developed, and the cultured Hippocampus has a trend to replace wild.

The sea horse cultivation is different from general aquaculture, and all the sea horse cultivation is fed with natural fresh and live baits, such as copepods, mysidacea and adult artemia, which are similar to the baits of the sea horse in the natural environment. The hippocampus mainly depends on copepods before 5cm, although artemia larvae can be partially replaced, the growth and survival effects of hippocampus seedlings are poor, and the copepods determine the success or failure of hippocampal culture to some extent, particularly for grey hippocampus. The hippocampus prefers to eat the philosophila fleas, does not eat the harpacia fleas, and the harpacia fleas are harmful to the hippocampus, and the newborn seedlings are put into the harpacia fleas and die in the fastest 20 minutes. The copepods required for cultivation are basically supplied in ponds, however, at a certain stage, the pond flea is propagated in a large amount, so that the harvested copepods cannot be fed to the hippocampus due to the high proportion of the flea, and the proportion of the flea and the philosophile reaches the level of 1. If the two copepods can be effectively separated, the method has important value for hippocampal culture.

The production of copepods is to be understood as the first step to grasp their ecological habits. The ecological habits of copepods are complex, the distribution in water is closely related to light, some have phototaxis, some have photophobia, and the phototaxis and photophobia are related to light intensity, namely, the phototaxis or photophobia is expressed under certain light intensity. The philosophila is a planktonic species which changes day and night in the water body, and the harpactica is mostly benthic species which are distributed in the middle and lower layers of the water body, so that a basic theoretical basis is provided for separating two copepods. However, although philosophila fleas are planktonic species, their phototaxis is very different. In natural waters, some species are shown to sink to the middle and lower layers of the water body in daytime and rise to the upper layers in the evening; some species are not sensitive to light intensity and only live on the upper layer; some upper, middle and lower water bodies are distributed. And the calamus philoxeroides and the harpacticoida are mixed and distributed in the pond, which shows that the harpacticoida also has certain phototaxis. In short, copepods respond to light with a wide variety of species. At present, the research on the aspects is very few, and particularly, the tendency and avoidance behavior of copepods to different bright lights are only seen in a few species such as Chinese philosophy fleas.

Disclosure of Invention

The invention aims to solve the technical problem of providing a method for separating philosophila and harpacticoida so as to fill the blank in the prior art.

The invention provides a method for separating philosophila and daphnia magna, which comprises the following steps of:

A. placing the collected copepods into a separation barrel, filling the barrel with water, and stopping inflating;

B. the upper part of the separation barrel is provided with illumination with the light intensity of 1000-1100lx;

C. standing, discharging part of water, collecting and checking the proportion of philosophy water fleas, then adding water, repeating the process, and abandoning the collected philosophy water fleas if the proportion of the collected philosophy water fleas in the copepods is below 5%;

D. if the proportion of the collected copepods is higher than 10%, putting the collected copepods into another separation barrel, and repeating the processes C and D until the proportion of the fleas in the discharged copepods is lower than 5%;

F. and finally, checking copepods in the separation barrel, and completely collecting copepods when the proportion of harpacticoida is less than 5%.

Preferably, the separation barrel in the step A is a conical bottom barrel similar to a artemia hatching barrel, the diameter of the separation barrel is 85-90 cm, the depth of the separation barrel is 75-85 cm, a valve is arranged at the cone bottom, the bottom of the inner cone and the lower part of the barrel body are white in 10-15 cm, the rest is black, and the outer part of the separation barrel is black.

Preferably, the LED illuminating lamp is arranged at the top of the separation barrel in the step B, and the light intensity is 1000-1100lx.

Preferably, the standing time in the step C is 8-10 minutes.

Preferably, the part of water discharged in the step C is: and opening a valve at the bottom of the barrel to discharge 20-25 cm of water.

Preferably, the philosophila is spindle water flea, and the harpacticoida is tiger magna.

Preferably, more than 95% of the copepods collected in step F are philosophila fleas and used to feed the hippocampus.

The light intensity related to the invention can enable the philosophila to have phototaxis and simultaneously enable the daphnia magna to generate light-resistant reaction.

Advantageous effects

The inventors have found that harpacticoida prefers to adhere to the walls of a container, especially to the walls of a white container. The biggest characteristic of the invention is that according to the characteristics of certain photophobia, benthic and white preference of the daphnia magna, the daphnia magna is separated out by a designed separation barrel under certain light intensity, so that the proportion of the daphnia magna in the separated copepods reaches more than 95%, the daphnia magna is reduced from 50% to less than 5%, and the daphnia magna can be safely fed to avoid the damage to the young seahorses.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.

Example 1

1. Separation barrel

The separating barrel is changed from an artemia hatching barrel, the volume of the barrel body is about 420 liters, the depth of the barrel is 85cm, white paint is brushed on the bottom and the lower part of the inner wall of the barrel by 10 cm, the rest is black, and the outer part of the barrel is black; an illuminating lamp is arranged above the separation barrel.

2. Separation process

The separation barrel is placed in a low light environment, and the light intensity is lower than 100lx. 2 jin (the weight after water filtration) of collected copepods are put into a separation barrel, and the separation barrel is filled with seawater without aeration; comparing the four water surface light intensities: 600-700lx, 1000-1100lx, 1400-1500lx and 1600-1700lx; standing for 8-10 minutes, completely opening a valve at the bottom of the bucket to discharge 20 cm of water, collecting and checking the proportion of the philosophy water and the fleas, then filling the water, and repeating the process; if the proportion of the collected calamus philadelphoides in the copepoda is lower than 5 percent, the collected calamus philadelphoides is discarded; if the proportion of collected calamus water fleas in the copepoda is higher than 10%, putting the collected copepoda into another separation barrel, and repeating the process until the proportion of the discharged calamus water fleas in the copepoda is lower than 5%; and finally, checking copepods in the separation barrel, and collecting copepods in the barrel to feed the hippocampus after the proportion of the harpacticoida is lower than 5%. The four light intensity experimental effects show that the separation times of the 1000-1100lx groups are the least, the flea harpacticoida can be separated to 4% only by 3 times, the 600-700lx and 1400-1500lx groups respectively need 4 and 5 times to reach 5%, and under the light intensity of 1600-1700lx, due to the light-shielding behavior of the philosophy flea, the flea harpacticoida and the flea at the lower part are mixed together and are difficult to separate. Feeding experiments prove that the young hippocampus cannot be harmed as long as the proportion of harpacticoida in copepods is lower than 5 percent. Wherein the philosophila flea is spindle flea, and the harpacticoida is tiger flea.

Comparative example 1

According to the embodiment 1, the inner side of the separation barrel is completely black, the light intensity is 1000-1100lx, the rest is the same as the embodiment 1, and the separation times are 5 times, so that the harpacticoida can be separated to 5 percent.

Comparative example 2

According to the embodiment 1, the inner side of the separation barrel is white, the light intensity is 1000-1100lx, the rest is the same as the embodiment 1, and the separation times are 6 times, so that the flea harpacticoides can be separated to 5 percent.

While the embodiments of the present invention have been described in detail, the present invention is not limited to the embodiments, and those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention, and such equivalent modifications or substitutions are intended to be included within the scope of the present invention as defined by the appended claims.

Claims

1. A method for separating philosophila and daphnia magna comprises the following steps:

C. standing, discharging part of water, collecting and checking the proportion of the philosophila to fleas, then adding water, repeating the process, and if the proportion of the philosophila to fleas in the collected copepods is below 5%, discarding;

F. and finally, checking copepods in the separation barrel, and completely collecting the copepods when the proportion of the harpacticoida is less than 5%.

2. The separation method of claim 1, wherein the separation barrel in step A is a cone bottom barrel with diameter of 85-90 cm and depth of 75-85 cm, the cone bottom is equipped with a valve, the inner cone bottom and the lower part of the barrel body are white 10-15 cm, the rest is black, and the outside of the barrel is black.

3. The separation method according to claim 1, wherein the illumination in step B employs an LED lamp as a light source.

4. The separation method according to claim 1, wherein the standing time in the step C is 8 to 10 minutes.

5. The separation process of claim 1, wherein the portion of the water withdrawn in step C is: and fully opening a valve at the bottom of the barrel to discharge 20-25 cm of water.

6. The separation method according to claim 1, wherein the philosophila is spindle water flea and the harpacticoida is tiger flea.

7. The isolation method according to claim 1, wherein more than 95% of the copepods collected in step F are fleas and used to feed the hippocampus.