CN1154849A - Method for preparation of puerarin - Google Patents
Method for preparation of puerarin Download PDFInfo
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- CN1154849A CN1154849A CN 96100953 CN96100953A CN1154849A CN 1154849 A CN1154849 A CN 1154849A CN 96100953 CN96100953 CN 96100953 CN 96100953 A CN96100953 A CN 96100953A CN 1154849 A CN1154849 A CN 1154849A
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Abstract
The present invention relates to a method for extracting puerarin, which is characterized by that it uses sliced dry pueraria root as raw material and uses ethyl alcohol to make extraction and concentration to obtain extract, uses n-butyl alcohol to dissolve the obtained extract, and makes said material pass through extractiont reatment; and makes the extract pass through such processes of decolouring by aluminium oxide column, concentrating under vacuum, filtering, drying and dissolving crystal so as to obtain the invented refined product. Said invention features simple technological process, convenient operation control and good product quality with purity of above 97%.
Description
The present invention relates to a kind of technology that adopts chemical industry method effective component extracting from plant, specifically it is a kind of method of extracting puerarin from Radix Puerariae.
Puerarin is a kind of raw material of producing medicine, and it is to extract from the root of pulse family Pueraria lobota platymiscium bavin Pueraria lobota.More for the method for from plant, extracting effective ingredient, it as application number the production technology of having introduced a kind of gypenoside in China's application for a patent for invention prospectus of 87106556.8, it is that gynostemma pentaphyllum herb is chopped up, and water or ethanol soaked charge level, heating extraction three times, merge extractive liquid,, reclaim ethanol, be condensed into flowing soaking paste, the cooling back adds ethanol, to no longer producing precipitation, more after filtration, concentrate, be drying to obtain thick Herb Gynostemmae Pentaphylli total glycosides.This extraction process mainly is to be used to extract Herb Gynostemmae Pentaphylli total glycosides, can't be directly used in the extraction puerarin.By retrieval, do not see the manufacturing technique method that is directly used in the extraction puerarin.
The purpose of this invention is to provide a kind of method of producing puerarin, and this method has better simply technical process, to adapt to the needs of carrying market.
Purpose of the present invention can reach by following scheme.The present invention is a raw material with the Radix puerariae heel piece, and main points of the present invention are, the Radix puerariae heel piece is put into the extraction equipment ethanol extraction, promptly soaked charge level lixiviate 1-5 time with 60%-95% ethanol, extraction temperature is 50-70 ℃, and each extraction time is 10-24 hour, merge lixiviating solution, be condensed into the extractum shape; Gained extractum is put into dissolution equipment with the n-butyl alcohol dissolving, promptly use water-saturated n-butanol and n-butyl alcohol, alternately dissolve 5-20 time at normal temperatures and pressures, stir after 0.5-2 hour at every turn and left standstill 1-3 hour, get its supernatant, and with each time supernatant mix homogeneously; The supernatant injection extraction equipment of mix homogeneously is extracted with water or n-butyl alcohol; Extract decolours through acid aluminium sesquioxide post, and through concentrating under reduced pressure, filtration, the dry semifinished product that gets; Semifinished product is put into dissolving crystallized equipment adding 90%-95% glacial acetic acid, and its adding weight is 8-15 times of semifinished product weight, and temperature adds to 90-118 ℃ and dissolves, and the dissolving postcooling leaves standstill crystallization, crystallization and filtration and the dry elaboration that gets.
The present invention has been owing to adopted ethanol leaching and the n-butanol extraction method, make the present invention have technological process simply, the characteristics of convenient operation and control, good product quality, thereby reached purpose of the present invention.
Below be several embodiments of the present invention, can further understand process of the present invention by embodiment.
Embodiment 1
1, feeds intake: get 200 kilograms of Radix puerariae heel pieces;
2, ethanol extraction: add 1000 kilograms of 75% ethanol for the first time, be warming up to 60 ℃, leached 20 hours; For the second time add 600 kilograms of 75% ethanol, be warming up to 58 ℃, leached 16 hours; Add 600 kilograms of 75% ethanol for the third time, be warming up to 55 ℃, leached 12 hours; Three immersion get 65 kilograms of extractum after concentrating recovery ethanol;
3, extractum dissolving: 65 kilograms of extractum are put into dissolving tank, alternately add dissolving 16 times with water-saturated n-butanol and commercial n-butyl alcohol respectively, each stirring and dissolving 1 hour left standstill 2 hours; Get the preceding supernatant mix homogeneously that leaves standstill for 11 times in order to extraction, afterwards leave standstill supernatant 5 times and be used for extractum dissolving in batches down;
4, extraction: adopt the stirring extraction tower to carry out the double tower counter-current extraction; The first tower axis rotating speed is that per minute 54 changes, and velocity of medium is: per hour 18 liters of supernatant, and per hour 72 liters in water, gained single extraction liquid is transparent, glassy yellow, no flocculent deposit; The second tower axis rotating speed is that per minute 84 changes, and velocity of medium is: per hour 72 liters of single extraction liquid, and per hour 36 liters of n-butyl alcohol, the gained extract is transparent, glassy yellow, no flocculent deposit;
5, this batch 1050 kilograms of extracts, through the decolouring of aluminium sesquioxide post,, after concentrated solution leaves standstill and separates out, get rid of filter and get 7.5 kilograms of semifinished products through natural drying again through concentrating under reduced pressure;
6, crystallization: get semifinished product 50 gram, add 175 milliliters in 95% glacial acetic acid, be heated to 116 ℃ of dissolvings, and crystallization gets elaboration 18.75 grams, the elaboration drying measures that it is up-to-standard, and content is 97.6%.
Claims (1)
1, a kind of method of producing puerarin, with the Radix puerariae heel piece is raw material, the invention is characterized in, the Radix puerariae heel piece is put into the extraction equipment ethanol extraction, promptly soaked charge level lixiviate 1-5 time with 60%-95% ethanol, extraction temperature is 50-70 ℃, and each extraction time is 10-24 hour, merge lixiviating solution, be condensed into the extractum shape; Gained extractum is put into dissolution equipment with the n-butyl alcohol dissolving, promptly use water-saturated n-butanol and n-butyl alcohol, alternately dissolve 5-20 time at normal temperatures and pressures, stir after 0.5-2 hour at every turn and left standstill 1-3 hour, get its supernatant, and with each time supernatant mix homogeneously; The supernatant injection extraction equipment of mix homogeneously is extracted with water or n-butyl alcohol; Extract decolours through acid aluminium sesquioxide post, and through concentrating under reduced pressure, filtration, the dry semifinished product that gets; Semifinished product is put into dissolving crystallized equipment adding 90%-95% glacial acetic acid, and its adding weight is 8-15 times of semifinished product weight, and temperature adds to 90-118 ℃ and dissolves, and the dissolving postcooling leaves standstill crystallization, crystallization and filtration and the dry elaboration that gets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96100953A CN1054068C (en) | 1996-01-18 | 1996-01-18 | Method for preparation of puerarin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96100953A CN1054068C (en) | 1996-01-18 | 1996-01-18 | Method for preparation of puerarin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1154849A true CN1154849A (en) | 1997-07-23 |
| CN1054068C CN1054068C (en) | 2000-07-05 |
Family
ID=5116831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96100953A Expired - Fee Related CN1054068C (en) | 1996-01-18 | 1996-01-18 | Method for preparation of puerarin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1054068C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064255C (en) * | 1997-09-01 | 2001-04-11 | 河北省宽城制药厂 | Preparation method of Yufengningxin tablets |
| CN1074673C (en) * | 1998-07-17 | 2001-11-14 | 山东绿叶制药股份有限公司 | Traditional Chinese medicine compositions contg. pueraria root extract |
| CN1300162C (en) * | 2004-04-02 | 2007-02-14 | 安康市志朗生物资源应用研究所 | Puerarin extracting method |
| CN104447719A (en) * | 2014-11-11 | 2015-03-25 | 常州大学 | Method for extracting puerarin from kudzu vine |
| CN107522692A (en) * | 2017-10-25 | 2017-12-29 | 贵州国农葛业有限公司 | A kind of method that waste liquid separating puerarin is produced using pueraria starch |
| CN111393422A (en) * | 2020-05-07 | 2020-07-10 | 四川轻化工大学 | Method for producing puerarin from radix Puerariae |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305888C (en) * | 2005-05-09 | 2007-03-21 | 何照范 | Method for producing puerarin in high purity and aglycon of soybean jointly |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1033914C (en) * | 1991-11-06 | 1997-01-29 | 河南省医学科学研究所 | Kudzu vine root extract, its preparation and use |
| CN1057093C (en) * | 1995-02-23 | 2000-10-04 | 赵爱平 | Extraction and application of Lobed Kudzuvine |
-
1996
- 1996-01-18 CN CN96100953A patent/CN1054068C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064255C (en) * | 1997-09-01 | 2001-04-11 | 河北省宽城制药厂 | Preparation method of Yufengningxin tablets |
| CN1074673C (en) * | 1998-07-17 | 2001-11-14 | 山东绿叶制药股份有限公司 | Traditional Chinese medicine compositions contg. pueraria root extract |
| CN1300162C (en) * | 2004-04-02 | 2007-02-14 | 安康市志朗生物资源应用研究所 | Puerarin extracting method |
| CN104447719A (en) * | 2014-11-11 | 2015-03-25 | 常州大学 | Method for extracting puerarin from kudzu vine |
| CN107522692A (en) * | 2017-10-25 | 2017-12-29 | 贵州国农葛业有限公司 | A kind of method that waste liquid separating puerarin is produced using pueraria starch |
| CN111393422A (en) * | 2020-05-07 | 2020-07-10 | 四川轻化工大学 | Method for producing puerarin from radix Puerariae |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1054068C (en) | 2000-07-05 |
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