CN115462371A - Fresh cut flower preservative and preparation method and application thereof - Google Patents
Fresh cut flower preservative and preparation method and application thereof Download PDFInfo
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- CN115462371A CN115462371A CN202211373798.5A CN202211373798A CN115462371A CN 115462371 A CN115462371 A CN 115462371A CN 202211373798 A CN202211373798 A CN 202211373798A CN 115462371 A CN115462371 A CN 115462371A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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Abstract
The invention belongs to the technical field of flower preservation, and particularly relates to a fresh cut flower preservative as well as a preparation method and application thereof. The fresh cut flower preservative comprises the following raw materials in parts by weight: 0.2 to 3 portions of organic acid, 0.1 to 0.5 portion of chitosan and 0.1 to 0.5 portion of polylysine. The chitosan and the polylysine in the fresh cut flower preservative provided by the invention have synergistic interaction, so that the permeability of cell membranes is increased, the antioxidant capacity of petals of fresh cut flowers can be improved, the size of the flower diameter of the nymphaea hybrid is maintained, the vase life of the nymphaea hybrid is prolonged, and the decline of the fresh cut flowers is prevented.
Description
Technical Field
The invention belongs to the technical field of flower preservation, and particularly relates to a fresh cut flower preservative as well as a preparation method and application thereof.
Background
With the improvement of living standard, the requirements of people on living quality are higher and higher, and the demand of flowers is also increased. In the fresh-keeping process of flowers, the florescence of the flowers is maintained as much as possible, and the shape of the flowers is kept.
The current fresh-keeping method for fresh flowers comprises a preservative of a bottle insert liquid, and refrigeration and air conditioning preservation. The preservative can provide nutrients for flowers in the bottle inserting process, but avoids the loss of the flowers in the transportation process. The fresh flowers are stored in a low-temperature environment for refrigeration and preservation, and the requirement on the environment is high and the temperature is too high or too lowThe temperature can cause damage to flowers, and the requirements of different types of flowers on temperature are obviously different. Controlled atmosphere preservation by controlling the storage environment of CO in the cut flowers 2 And O 2 The content of the active ingredients in the fresh-keeping agent reduces the respiration rate of flowers, slows down the consumption of nutrients and energy in the fresh-keeping process of the fresh cut flowers, prolongs the vase life of the fresh cut flowers, and has high requirements on the environment and higher cost. The refrigeration fresh-keeping and the air-conditioning fresh-keeping are fresh-keeping methods suitable for the transportation process and are not suitable for daily bottle insertion.
The nymphaea hybrid is tropical large-scale nymphaea candida belonging to Nymphaeaceae, is perennial root aquatic flower, is bright in color, fresh and natural in taste and has high ornamental value. However, the vase service life of the nymphaea hybrid is short, petals are easy to fall off, and the nymphaea hybrid has an ornamental period of 2-3d after being picked, so that the development of the nymphaea hybrid in the market of fresh cut flowers is severely limited.
The film coating preservation is to uniformly coat a macromolecule liquid film on the surface of the fresh flower, and the macromolecule liquid film can be attached to the surface of the fresh flower after being dried to form a transparent film, so that the film can reduce the direct contact between the fresh flower and air, avoid the water evaporation of the fresh flower, reduce the consumption of respiration and nutrient substances, and reduce the phenomena of fresh flower decay and the like caused by pathogenic bacteria infection. The coating preservative has less research on the coating preservation of the cut flowers, and after the flowers are coated, the stability of petals can be improved by utilizing the bonding effect of the coating agent, so that the ornamental time is prolonged.
In conclusion, the film coating agent suitable for the transportation process and the bottle-inserting preservation of the fresh cut flowers is developed.
Disclosure of Invention
In view of the above disadvantages of the prior art, the present invention aims to provide a cut flower preservative, a preparation method and a use thereof, which are used for alleviating the phenomena of easy fall, dehydration and wilting of petals of the adopted nymphaea hybrid and maintaining the length of flower stems of the nymphaea hybrid when the nymphaea hybrid blossoms bloom.
In order to achieve the purpose, the invention adopts the following technical scheme.
The first aspect of the invention protects a fresh cut flower preservative which comprises the following raw materials in parts by weight:
0.2 to 3 portions of organic acid
0.1 to 0.5 portion of chitosan
0.1-0.5 part of polylysine.
The fresh cut flower preservative is compounded by chitosan and polylysine, and the chitosan and the polylysine have a synergistic effect. Malondialdehyde (MDA) is a product of membrane lipid peroxidation, and compared with the independent chitosan coating treatment, the chitosan composite polylysine treatment increases the excessive accumulation of MDA, avoids the increase of membrane permeability, improves the stability of cell membranes, and maintains the strong oxidation resistance of the fresh cut flowers, thereby preventing the decay of the fresh cut flowers, better maintaining the flower diameter of the fresh cut flowers, and better prolonging the vase life of the fresh cut flowers.
In some embodiments, the organic acid may be present in an amount of 0.2 to 1.0 part by weight, 0.5 to 1.5 parts by weight, 1.2 to 2.5 parts by weight, or 2.2 to 3 parts by weight. In a preferred embodiment, the amount is 1.0 part.
In some embodiments, the chitosan may be present in an amount of 0.1 to 0.22 parts by weight, or 0.20 to 0.30 parts by weight, or 0.25 to 0.41 parts by weight, or 0.32 to 0.5 parts by weight. In a preferred embodiment, the amount is 0.25 parts.
In certain embodiments, the polylysine is present in an amount of 0.1 to 0.22 parts by weight, alternatively 0.20 to 0.30 parts by weight, alternatively 0.25 to 0.41 parts by weight, alternatively 0.32 to 0.5 parts by weight. In a preferred embodiment, the amount is 0.25 parts.
In certain embodiments, the organic acid is selected from one or more of acetic acid, citric acid, salicylic acid, sorbic acid, and ascorbic acid. The organic acid can reduce the pH value of the solution, inhibit the growth of microorganisms, prevent the cauliflower vascular bundle from being blocked and promote the water absorption of the flowering branches.
Preferably, the organic acid is selected from acetic acid. The compatibility of acetic acid, chitosan and polylysine has more remarkable effects on MDA content, antioxidase and in-vivo and in-vitro oxidation resistance, and is superior to other organic acids.
In some embodiments, the chitosan is a natural product formed from chitosan deacetylation, and the molecular formula is (C) 6 H 11 NO 4 ) n The molecular weight of the unit body is: 161.2g/mol.
In certain embodiments, the polylysine is selected from alpha-polylysine (APL) and/or epsilon-polylysine (EPL).
In certain embodiments, the polylysine has a weight average molecular weight of 3500 to 70000Da.
Preferably, the polylysine is epsilon-polylysine (EPL), and the molecular weight of the polylysine is 10000-70000 Da.
In certain embodiments, the cut flower preservative further comprises water.
Preferably, the concentration of the acetic acid is 0.5 to 1.5v/v% based on the total volume of the organic acid, chitosan, polylysine and water.
More preferably, the concentration of acetic acid may be 0.5 to 0.9v/v%, or may be 0.8 to 1.2v/v%, or may be 1.0 to 1.5v/v%. In a preferred embodiment, the concentration is 1.0v/v%.
Preferably, the concentration of the chitosan is 0.1 to 0.5w/v% based on the total volume of the organic acid, chitosan, polylysine and water.
More preferably, the concentration of the chitosan may be 0.1 to 0.28 w/v%, or may be 0.15 to 0.38 w/v%, or may be 0.35 to 0.5w/v%. In a preferred embodiment, the concentration is 0.25 w/v%.
Preferably, the polylysine is present in a concentration of 0.1 to 0.5w/v%, based on the total volume of organic acid, chitosan, polylysine, and water.
More preferably, the concentration of polylysine may be 0.1 to 0.28 w/v%, or may be 0.15 to 0.38 w/v%, or may be 0.35 to 0.5w/v%. In a preferred embodiment, the concentration is 0.25 w/v%.
The second aspect of the present invention protects the preparation method of the cut flower preservative as described above, comprising the steps of:
mixing acetic acid, chitosan, polylysine and water to obtain the fresh cut flower preservative.
In certain embodiments, acetic acid and water are mixed first, chitosan is added and mixed, and polylysine is added and mixed.
A third aspect of the present invention protects the use of a cut flower preservative as described above in the preservation of cut flowers.
In certain embodiments, use in at least one of:
1) The vase life of the fresh cut flowers is prolonged;
2) Improving the enzyme activity of the oxidase of the fresh cut flowers;
3) Reducing the malondialdehyde content of the fresh cut flowers;
4) Improving the in-vivo and in-vitro oxidation resistance of the fresh cut flowers.
The fourth aspect of the invention provides a method for preserving fresh cut flowers, wherein the fresh cut flower preservative is sprayed on the fresh flowers.
In some embodiments, the cut flower preservative is placed in an atomizer and sprayed on the surface of the cut flower. So that a uniform protective film is formed.
A fifth aspect of the present invention protects a cut flower preservative as described above or a method of preparation as described above or a use as described above or a method as described above, the cut flower being selected from one or more of lotus, hydrangea, rose and rose.
Preferably, the lotus is a nymphaea hybrid.
The chitosan raw material adopted by the invention has wide sources, is natural and non-toxic, has good biocompatibility, biodegradability, film forming property and antibacterial property, and is widely applied to the film preservation of foods such as fruits and vegetables, meat products, aquatic products and the like.
Epsilon-polylysine (Epsilon-PL) is a natural preservative of microbial source, has broad-spectrum antibacterial activity on most gram-negative and gram-positive bacteria, fungi and viruses, has the advantages of safety, no toxicity, good biodegradability, high-temperature stability and the like, has good preservative effect on dairy products, meat products, marine products and starch foods, and has simple preparation process, low cost and good effect. The epsilon-polylysine, the glycine, the Nisin, the natamycin, the chitosan and the like have a synergistic antibacterial effect and are widely applied to food fresh-keeping and medical antibacterial products, and the chitosan and the epsilon-PL have an obvious synergistic antibacterial effect or can be complemented in antioxidant property.
Compared with the prior art, the invention has the following beneficial effects:
1) The fresh cut flower preservative is compounded by chitosan and polylysine, and the chitosan and the polylysine have a synergistic effect. Malondialdehyde (MDA) is a product of membrane lipid peroxidation, and compared with the treatment of singly adopting chitosan to coat, the treatment of chitosan and polylysine increases the excessive accumulation of MDA, avoids the increase of membrane permeability, improves the stability of cell membranes, and maintains the strong oxidation resistance of the fresh cut flowers, thereby preventing the decay of the fresh cut flowers, better maintaining the flower diameter of the fresh cut flowers, and better prolonging the vase life of the fresh cut flowers.
2) After the fresh cut flower preservative is sprayed on the fresh cut flowers, the in-vivo and in-vitro oxidation resistance reduction rate is slowed down, the decay of the fresh cut flowers is delayed, and the ornamental value of the fresh cut flowers is increased.
3) The fresh cut flower preservative has the advantages of simple preparation process, safety, harmlessness, simple formula and low cost, increases the economic and ornamental values and the practical values of the fresh cut flower preservative, and has good development and application potentials.
Drawings
FIG. 1 is a graph showing the change of the flower diameter of the nymphaea hybrid of the chitosan group, the composite coating group and the control group with time in the application example of the present invention.
Fig. 2 is a graph showing changes in the water balance values of the nymphaea hybrid of the chitosan group, the composite coating film group, and the control group in the application example of the present invention.
Fig. 3 is a graph showing the change of MDA content of the nymphaea hybrid of the chitosan group, the composite coating group, and the control group in the application example of the present invention.
FIG. 4 is a graph showing the effect of CAT enzyme activity of nymphaea hybrid of chitosan group, composite coating group and control group in the application example of the present invention.
Fig. 5 is a graph showing the effect of POD enzyme activity of nymphaea hybrid of the chitosan group, composite coating group, and control group in the application example of the present invention.
FIG. 6 is a graph showing the effect of SOD enzyme activity of nymphaea hybrid of chitosan group, composite coating group and control group in the application example of the present invention.
FIG. 7 is a graph showing the in vitro antioxidant capacity of the nymphaea hybrid of the chitosan group, the composite coating group and the control group in the application example of the present invention.
Fig. 8 is a graph showing the in vivo antioxidant capacity of nymphaea hybrid of the chitosan group, composite coating group and control group in the application example of the present invention.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention. Test methods in which specific conditions are not noted in the following examples are generally performed under conventional conditions or conditions recommended by each manufacturer.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.
Example 1
In this example, the preparation and obtaining of the cut flower preservative comprises the following steps:
the formula of the fresh cut flower preservative comprises the following components: 1 part of organic acid, 0.25 part of chitosan and 0.25 part of epsilon-polylysine.
Adding acetic acid into deionized water, stirring uniformly, and preparing into an acetic acid aqueous solution with the volume fraction of 1 wt%.
2.5g of chitosan is weighed and added into 1L of 1wt% acetic acid water solution, fully stirred, ultrasonically dissolved, kept stand and defoamed to prepare 0.25wt% chitosan-acetic acid solution.
Weighing 200 mL of 0.25wt% chitosan-acetic acid solution, adding 0.5g of polylysine, uniformly stirring, and fully dissolving to obtain the fresh cut flower preservative.
Example 2
In this example, the preparation and obtaining of the cut flower preservative comprises the following steps:
the formula of the fresh cut flower preservative comprises the following components: 1.5 parts of organic acid, 0.3 part of chitosan and 0.3 part of epsilon-polylysine.
Adding acetic acid into deionized water, and stirring uniformly to prepare an acetic acid aqueous solution with the volume fraction of 1.5 wt%.
Weighing 3g of chitosan, adding the chitosan into 1L of 1.5wt% acetic acid aqueous solution, fully stirring, performing ultrasonic treatment to completely dissolve the chitosan, standing and defoaming, and preparing 0.3wt% chitosan-acetic acid solution.
200 mL of 0.3wt% chitosan-acetic acid solution is measured, 0.6 g polylysine is added, and the fresh cut flower preservative is obtained after uniform stirring and full dissolution.
Example 3
In this example, the preparation and obtaining of the cut flower preservative comprises the following steps:
the formula of the fresh cut flower preservative comprises the following components: 1 part of organic acid, 0.2 part of chitosan and 0.2 part of epsilon-polylysine.
Adding acetic acid into deionized water, and stirring uniformly to prepare an acetic acid aqueous solution with the volume fraction of 1 wt%.
Weighing 2g of chitosan, adding the chitosan into 1L of 1wt% acetic acid aqueous solution, fully stirring, performing ultrasonic treatment to completely dissolve the chitosan, standing and defoaming, and preparing 0.2wt% chitosan-acetic acid solution.
200 mL of 0.2wt% chitosan-acetic acid solution is measured, 0.4g polylysine is added, and the fresh cut flower preservative is obtained after uniform stirring and full dissolution.
Comparative example 1
The difference between the comparative example and the example 1 is that the 0.25wt% chitosan-acetic acid solution obtained in the example 1 is directly used as the fresh cut flower preservative without adding polylysine.
Comparative example 2
The difference of the comparative example from the example 1 is that no chitosan is added, 200 mL of the 1wt% acetic acid aqueous solution obtained in the example 1 is measured, 0.5g of polylysine is added, and the fresh cut flower preservative is obtained after uniform stirring and full dissolution.
Application example
In the application example, the nymphaea hybrid is taken as a research object, and the fresh-keeping research is carried out by respectively adopting the fresh-keeping agents obtained in the example 1 and the comparative example 1.
1. Experimental methods
1.1 grouping
Selecting 32 fresh and open nymphaea hybrid with similar growth conditions, and 8 nymphaea hybrid groups.
Composite coating film group: the preservative obtained in the example 1 is placed in an atomizer, the surface of the nymphaea hybrid is coated with the preservative by the atomizer to form a layer of uniform protective film, then the protection film is inserted into equivalent deionized water to be stored at room temperature, and the deionized water is replaced every day to keep the liquid level constant.
Coating a chitosan film group: the preservative obtained in the comparative example 1 is placed in an atomizer and sprayed on the surface of the nymphaea hybrid, and the rest are all the same as the composite coating group.
Polylysine coating set: the preservative obtained in the comparative example 2 is placed in an atomizer and sprayed on the surface of the nymphaea hybrid, and the rest are all the same as the composite coating group.
Blank group: the preservative is replaced by deionized water, and the rest components are the same as the composite coating group.
Samples from each treatment group were taken at 0, 2, 4 and 6d for each index measurement. The freshness-keeping effect of the nymphaea hybrid is judged by measuring the bottle-inserting life, the flower diameter, the water balance value, the MDA content, the enzyme activity and the change of the in-vivo and in-vitro oxidation resistance of the nymphaea hybrid.
1.2 index measurement method
1) The service life of the bottle insert is as follows: the color of the petals, the back of the petals, the severe water loss and wilting and the loss of ornamental value are taken as the signs of the end of the life of the arranged flowers.
2) The flower diameter is as follows: the maximum diameter of each flower was measured twice by a cross measurement method with a vernier caliper at about 16: 00 per day, and the average value was taken. The flower diameter is not measured when half of each repetition loses ornamental value.
3) Determination of the moisture balance value:
and measuring the water loss and water absorption of the lotus flowers every ten am every day, and calculating the water balance value.
Flowering branch water loss (g) = (bottle mass on the day + solution mass on the day + flowering branch mass on the day) - (bottle mass on the next day + solution mass on the next day + flowering branch mass on the next day); flower branch water absorption (g) = (bottle mass on the day + solution mass on the day) - (bottle mass on the day after + solution mass on the day after)
Water balance value (g) = water absorption-water loss
4) And (3) MDA content determination:
the assay was performed with reference to Shanghai Biotech kit.
5) And (3) enzyme activity determination:
CAT, POD and SOD enzyme activities were measured with reference to the kit.
6) And (3) measuring in vivo and in vitro antioxidant capacity:
in vitro antioxidant assay comprises ABTS free radical scavenging ability assay and DPPH free radical scavenging ability assay
i. ABTS free radical scavenging Capacity assay
Weighing certain mass of lotus aqueous extracts with different bottle-inserting days, respectively preparing sample solutions with different concentrations (0.1-2.5 mg/mL) and positive control Vc into solutions with concentrations of 5.0, 1.0, 0.5, 0.1, 0.01 and 0.001 mmol/mL, respectively taking 50 mu L of each solution, adding the solution into each hole of a 96-hole cell culture plate, then respectively taking 150 mu L of ABTS + solution, and adding the solution into each hole of the 96-hole cell culture plate. After incubation at room temperature for 6 min, the absorbance at 734 nm (A) was measured and the clearance and IC50 values were calculated.
Clearance =[A 0 －(A 1 －A 2 )]/A 0
A 0 、A 1 AndA 2 ABTS + solutions representing blank solutions respectivelyATest sample and ABTS + mixed solutionAAnd of test samplesA。
Measurement of DPPH radical scavenging Capacity
Weighing certain mass of lotus aqueous extracts with different bottle insertion days, and respectively preparing sample solutions with different concentrations (0.1-2.5 mg/mL) and positive control Vc into solutions of 10.0, 5.0, 1.0, 0.5, 0.1, 0.01 and 0.001 mmol/mL. 50. Mu.L of each solution was added to each well of a 96-well cell culture plate, and 130. Mu.L of DPPH solution was added to each well of the 96-well cell culture plate. Standing at room temperature in dark place for 30 min, detecting absorbance at 517 nm, and calculating clearance rate in the same way as ABTS + free radical scavenging activity.
In vivo antioxidant capacity determination based on caenorhabditis elegans: n is to be 2 After the synchronization of wild type caenorhabditis elegans, the wild type caenorhabditis elegans is exposed to lotus aqueous extracts with different bottle insertion days of 0.1 mg/mL, and the determination of the activities of superoxide dismutase (SOD), catalase (CAT) and Glutathione (GSH) of the caenorhabditis elegans is respectively carried out according to the requirements of the specification of a kit produced by Nanjing institute of built bioengineering.
2. Results of the study
2.1 Research on flower diameter of nymphaea hybrid by preservative
The maximum flower diameter of the nymphaea hybrid was measured at 0d, 2d, 4d and 6d, respectively.
FIG. 1 is a graph of the effect of different treatment groups on the flower diameter of a nymphaea hybrid.
As can be seen from FIG. 1, the decrease of the nymphaea hybrid of the control group is fast, the state is worst, and the ornamental value is low; the chitosan coating and the polylysine coating improve the shape of the nymphaea hybrid to a certain extent; the diameters of the nymphaea hybrid of the composite coating group are relatively stable in 2d, 4d and 6d, are not obviously reduced, and the state of the nymphaea hybrid of the composite coating is optimal and the ornamental value is highest.
2.2 study of the Water balance value of the preservative on the nymphaea hybrid
Fig. 2 is a graph of the water balance value of the nymphaea hybrid for different treatment groups.
As can be seen from fig. 2, the water balance values of the control group, the chitosan coating film, the polylysine coating film and the composite coating film group were all positive values in 0-2d, but the water balance values of the control group and the chitosan coating film group were decreased to negative values in 4d, and the water balance values of the composite coating film group were decreased to negative values only in 6 d.
The water balance value directly reflects the utilization condition of the cut flowers of the nymphaea hybrid to water, and the composite coating treatment improves the water condition in the cut flowers, so that the withering process of the petals caused by water loss is delayed.
2.3 research on the content of MDA in nymphaea hybrid by the preservative
Fig. 3 is a graph of MDA content of nymphaea hybrid for different treatment groups.
Malondialdehyde (MDA) acts as a product of membrane lipid peroxidation, which behaves in accordance with cell membrane permeability. In the aging process, the MDA is excessively accumulated due to disturbance of the active oxygen scavenging system, so that the membrane permeability is increased.
As can be seen from fig. 3, the blank MDA growth amount and growth rate were higher than those of each treatment group, and the composite coating film group was grown less than those of the other coating film groups. The composite coating film group can better eliminate the accumulation of MDA, maintain the permeability of cell membranes, increase the stability of the cell membranes and slow down the aging.
2.4 study of enzymatic Activity of the preservative on the nymphaea hybrid
Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) are important protective enzymes in plants, can effectively remove free radicals in vivo and maintain normal operation of organisms, and play an important role in storage and fresh keeping of fresh flowers after being picked.
FIG. 4 is a graph of Catalase (CAT) of different treatment groups on the nymphaea hybrid.
Fig. 5 is a graph of Peroxidase (POD) of the nymphaea hybrid for different treatment groups.
Fig. 6 is a graph of superoxide dismutase (SOD) of nymphaea hybrid for different treatment groups.
As can be seen from FIGS. 4, 5 and 6, when the cut flower is just picked, more CAT, POD and SOD may be produced due to stress reaction, and then there is a descending process, as the bottle insertion time increases, senescence begins, the CAT, POD and SOD gradually rise to resist senescence to the late stage, the physiological function of the plant decreases all around, and the activities of CAT, POD and SOD gradually decline. According to the preservative group, three enzymes including CAT, POD and SOD are all in higher activity levels, and the effect is better than that of single coating treatment, so that the preservative can improve the activity of antioxidase of flower petals, improve the quality and the service life of flowers, and further delay the senescence of the flowers.
2.5 study of in vivo and in vitro antioxidant capacity of the preservative on nymphaea hybrid
FIG. 7 is the DPPH IC of different treatment groups for nymphaea hybrid 50 A graph of (a).
FIG. 8 is the IC of ABTS of nymphaea hybrid for different treatment groups 50 A graph of (a).
TABLE 1 comparison of in vitro antioxidant Capacity of nymphaea hybrid
It can be comprehensively known from fig. 7, fig. 8 and table 1 that the reduction rate of the in-vivo and in-vitro oxidation resistance of the nymphaea hybrid membrane group is smaller than that of the chitosan membrane, the polylysine group and the blank group, and the composite membrane group has the lowest influence on the oxidation resistance of the nymphaea hybrid membrane group, which indicates that the preservative can improve the oxidation resistance of the cut flowers and reduce the oxidation loss, which is possibly related to the stability of epsilon-polylysine.
2.5 vase life study of the preservative on the nymphaea hybrid
The vase life of the nymphaea hybrid is the days from the current day of vase cutting to the end of serious withering, shrinkage, fading, elbow and the like of petals (the indexes are the same below).
TABLE 2 perfume lotus flower Life
As can be seen from Table 2, the vase life of the nymphaea hybrid of the blank group, the chitosan coating group and the polylysine group is not significantly different, while the composite coating significantly improves the vase life of the nymphaea hybrid, and in addition, the effects of promoting flower blooming, delaying senescence and inhibiting petal shedding can be realized.
In conclusion, the chitosan/polylysine composite coating liquid serving as a green, efficient and safe preservation technology prolongs the bottle insertion time of the fresh cut flowers and improves the ornamental value, the in-vivo and in-vitro oxidation resistance reduction rate of the coated fresh cut flowers is slowed down, and the chitosan/polylysine composite coating can prolong the preservation time of the fresh cut flowers, delay the reduction of the oxidation resistance of the fresh cut flowers and increase the economic ornamental value and the practical value of the fresh cut flowers.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art can modify or change the above-described embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and scope of the present invention as defined in the appended claims.
Claims (10)
1. The fresh cut flower preservative is characterized by comprising the following raw materials in parts by weight:
0.2 to 3 portions of organic acid
0.1 to 0.5 portion of chitosan
0.1-0.5 part of polylysine.
2. The cut flower preservative according to claim 1, characterized by comprising at least one of the following technical characteristics:
1) The organic acid is selected from one or more of acetic acid, citric acid, salicylic acid, sorbic acid and ascorbic acid;
2) The chitosan is a natural product formed by removing N-acetyl from chitin, and the molecular formula is (C) 6 H 11 NO 4 ) n The molecular weight of the unit body is: 161.2g/mol;
3) The polylysine is selected from alpha-polylysine or epsilon-polylysine;
4) The weight-average molecular weight of the polylysine is 3500-70000 Da;
5) The fresh cut flower preservative also comprises water.
3. The fresh cut flower preservative according to claim 2, wherein the polylysine is epsilon-polylysine (EPL), and the molecular weight of the polylysine is 10000-70000 Da.
4. The fresh cut flower preservative according to claim 2, characterized by comprising at least one of the following technical characteristics:
5-1) taking the total volume of the organic acid, the chitosan, the polylysine and the water as a reference, wherein the concentration of the acetic acid is 0.5-1.5 v/v%;
5-2) taking the total volume of the organic acid, the chitosan, the polylysine and the water as a reference, wherein the concentration of the chitosan is 0.1-0.5 w/v%;
5-3) the polylysine has a concentration of 0.1 to 0.5w/v% based on the total volume of the organic acid, the chitosan, the polylysine and the water.
5. The preparation method of the fresh cut flower preservative according to any one of claims 1 to 4, characterized by comprising the following steps:
mixing acetic acid, chitosan, polylysine and water to obtain the fresh cut flower preservative.
6. A process according to claim 5, wherein the acetic acid and water are mixed, chitosan is added and mixed, and polylysine is added and mixed.
7. Use of a cut flower preservative according to any one of claims 1-4 for preserving cut flowers.
8. Use according to claim 7, characterized in that it comprises at least one of the following:
1) The vase life of the fresh cut flowers is prolonged;
2) Improving the enzyme activity of the oxidase of the fresh cut flowers;
3) Reducing the malondialdehyde content of the fresh cut flowers;
4) Improving the in-vivo and in-vitro oxidation resistance of the fresh cut flowers.
9. A method for keeping fresh of cut flowers, characterized in that the cut flower preservative according to any one of claims 1 to 4 is sprayed on the cut flowers.
10. Fresh-cut flower preservative according to any one of claims 1 to 4 or the method of preparation according to any one of claims 4 to 5 or the use according to claim 5 or the method according to claim 9, wherein the fresh-cut flower is selected from one or more of lotus, hydrangea, rose and rose.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116098145A (en) * | 2023-04-11 | 2023-05-12 | 云南建投物流有限公司 | Method for prolonging storage period of cut rose flowers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116098145A (en) * | 2023-04-11 | 2023-05-12 | 云南建投物流有限公司 | Method for prolonging storage period of cut rose flowers |
| CN116098145B (en) * | 2023-04-11 | 2023-06-20 | 云南建投物流有限公司 | Method for prolonging storage period of cut rose flowers |
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