CN115449462A

CN115449462A - Microbial flora storage device and storage method thereof

Info

Publication number: CN115449462A
Application number: CN202210994327.XA
Authority: CN
Inventors: 朱峰; 朱林; 班文洁
Original assignee: Anhui Mount Everest Biological Co ltd
Current assignee: Anhui Mount Everest Biological Co ltd
Priority date: 2022-08-18
Filing date: 2022-08-18
Publication date: 2022-12-09

Abstract

The invention discloses a microbial flora storage device and a microbial flora storage method, and the microbial flora storage device comprises a tank body, wherein a sealing door is arranged at the top of the tank body, the inner wall of the tank body is rotatably connected with an outer shaft sleeve, a transmission gear is fixedly arranged on the peripheral side surface of the outer shaft sleeve, the inner wall of the outer shaft sleeve is rotatably connected with an inner coil, the inner wall of the inner coil is rotatably connected with a cloth cooling shaft barrel, the peripheral side surface of the tank body is respectively and fixedly provided with a servo motor and a refrigeration mechanism, the output shaft end of the servo motor is respectively and rotatably connected with the outer shaft sleeve and the inner coil, the port of the refrigeration mechanism is fixedly communicated with the cloth cooling shaft barrel, the peripheral side surface of the cloth cooling shaft barrel is provided with a plurality of groups of air supply holes distributed in a circumferential array, and the peripheral side surface of the inner coil is fixedly provided with a main rotary seat. The device can efficiently finish the storage and culture operation of microbial flora through the arrangement of the structures such as the placement module, the refrigerating mechanism and the like, and the static storage structure of the traditional storage device is changed into the dynamic storage structure when the microbial flora is stored.

Description

Microbial flora storage device and storage method thereof

Technical Field

The invention relates to the technical field of flora storage devices, in particular to a microbial flora storage device and a microbial flora storage method.

Background

The human gastrointestinal tract is inhabited by a wide variety of microorganisms, which are called intestinal flora, the intestinal flora is combined according to a certain proportion, and the bacteria are restricted and dependent with each other, so that an ecological balance is formed on the quality and quantity.

The intestinal flora, which responds to nutrients consumed by the host and also responds to the state of the host by various hormones, can also produce compounds for signaling the human body system, including neurotransmitters such as gamma-aminobutyric acid, amino acids such as tyrosine and tryptophan, molecules which can be converted into emotions, dopamine and 5-hydroxytryptamine, and many other substances which have an important effect on human health, and once a plurality of diseases are caused by lack of such nutrients, a low-temperature storage operation is required for the flora during the cultivation of the intestinal flora, and the existing storage device is liable to suffer from uneven cooling during the storage operation, and on the other hand, the distribution of the flora in the culture solution is not uniform during the storage, and based on this, the present invention provides a microbial flora storage device and a storage method thereof, so as to solve the problems presented in the background art.

Disclosure of Invention

Technical problem to be solved

In view of the disadvantages of the prior art, the present invention aims to provide a microbial flora storage device and a storage method thereof, which solve the problems that the microbial flora is easily cooled unevenly during the storage operation of the conventional storage device, and the distribution of the microbial flora in the culture solution is uneven during the storage.

(II) technical scheme

In order to achieve the above object, the present invention provides the following technical solutions, wherein a microbial flora storage device adopts the following technical solutions: the utility model discloses a cooling device, including a jar body, the top of jar body is installed and is sealed a door, the inner wall of the jar body rotates and is connected with the outer axle sleeve, the week side fixed mounting of outer axle sleeve has drive gear, the inner wall of outer axle sleeve rotates and is connected with interior coil, the inner wall of interior coil rotates and is connected with the cloth cooling barrel, week side fixed mounting respectively of the jar body has servo motor and refrigeration mechanism, servo motor's output axle head is connected with outer axle sleeve and interior coil transmission respectively, refrigeration mechanism's port and the fixed intercommunication of cloth cooling barrel, week side of cloth cooling barrel has seted up a plurality of groups and has been the blast hole that circumference array distributes, week side of interior coil is equipped with the main seat of revolving admittedly, week side of main seat is connected with jar body rotation, the inner wall fixed mounting of main seat has a set ofly to be the module of placing that circumference array distributes, a set of placing the inner wall of module all is connected with the drive gear transmission, the bottom fixed mounting of the jar body has and places module complex excitation mechanism, the upper portion fixed mounting of the jar body has supplementary module.

As preferred scheme, place the module and include the seat of autogyration respectively and install in the lower ring gear of main seat of gyration bottom surface, the week side of seat of autogyration is rotated with the main seat of gyration and is connected, the top surface fixed mounting of seat of autogyration has the last ring gear of being connected with the drive gear transmission, the inner wall of seat of autogyration rotates and is connected with a set of cylinder of placing that is circumference array distribution, every place the equal fixed mounting in all sides of cylinder of gyration and have driven gear, driven gear's all sides are connected with lower ring gear transmission, the inner wall joint of placing the cylinder of gyration has top open-ended fungus tube of cultivateing, the bottom surface axis position of seat of autogyration rotates and is connected with the gas injection double-layered pipe, the fixed intercommunication in all sides of gas injection double-layered pipe has a set of clamp bag that is circumference array distribution, every the all sides of clamp bag cooperate with the fungus tube of cultivateing, the bottom and the rotation intercommunication of gas injection mechanism of clamp pipe.

As preferred scheme, excitation mechanism includes air pump, air guide revolves seat, electromagnetic ring and excitation section of thick bamboo respectively, the top surface and the jar body fixed connection of air pump, the air guide revolves the week side of seat and jar body rotation to be connected, the one end and the air guide of air pump gas outlet revolve the seat and rotate the intercommunication, the top surface and the air injection double-layered pipe rotation intercommunication of air guide revolves the seat, the bottom and the air guide of cloth cold axle section of thick bamboo revolve the seat and rotate and be connected, the inner wall of excitation section of thick bamboo just correspond every equal fixed mounting in position of placing the module bottom and have the filler ring, the inner wall and the air guide of excitation section of thick bamboo revolve seat sliding connection, install a set of resistance to compression spring that is circumferential array and distributes between the relative surface of excitation section of thick bamboo and air guide revolve the seat, the bottom surface fixed mounting of excitation section of thick bamboo have with electromagnetic ring complex permanent magnetism ring, the electromagnetic ring establish in the below of permanent magnetism ring just the bottom surface and jar body fixed connection of electricity.

As preferred scheme, the fixed baroceptor that is provided with in intercommunication department that the air pump revolves the seat with the air guide, the air guide revolves the seat and is hollow structure, the week side fixed mounting of cylinder that shakes has the sealing washer with jar body laminating, the inner wall of cylinder that shakes is fixed set up revolve seat sliding fit's guiding hole with the air guide, the cross section of guiding hole is regular polygon, the air guide revolves the fixed guide pin bushing that is provided with guiding hole shape adaptation in bottom of seat.

Preferably, the magnetism of the electromagnetic ring is the same as that of the permanent magnet ring.

As a preferred scheme, the refrigerating mechanism comprises a refrigerator fixedly connected with the tank body, a refrigerating annular cavity arranged in the tank body, a heat insulating layer arranged on the outer side of the tank body and a refrigerating coil arranged on the inner wall of the refrigerating annular cavity, one end of an air return port of the refrigerator is communicated with an air outlet of the refrigerating coil, one end of an air outlet of the refrigerator is communicated with an air supply main pipe, one end of the air outlet of the air supply main pipe is communicated with an air inlet of the refrigerating coil, the top end of the air supply main pipe is fixedly communicated with an air supply branch pipe, and the peripheral side face of the air supply branch pipe is fixedly communicated with the cold distribution shaft cylinder.

As the preferred scheme, the auxiliary module respectively include with jar body fixed connection's automatically controlled seat, install in the inside of jar body top temperature probe and oxygen concentration sensor, the inside of automatically controlled seat embeds there is the singlechip, temperature probe and oxygen concentration sensor's monitoring end all extends to the inside of jar body, temperature probe and oxygen concentration sensor's data end all is connected with the singlechip electricity, the top of jar body is fixed mounting respectively has into oxygen pipe and blast pipe, the pneumatic valve is all installed to the inside of advancing oxygen pipe and blast pipe.

According to the preferable scheme, the outer shaft sleeve and the inner rotating pipe are both hollow tubular structures with openings at two ends, driven bevel gears are fixedly mounted on the peripheral side surfaces of the outer shaft sleeve and the inner rotating pipe, two driving bevel gears are mounted at the output shaft end of the servo motor, the peripheral side surfaces of the two driving bevel gears are respectively meshed with the two driven bevel gears, and the two driven bevel gears are symmetrically arranged by taking the horizontal plane where the axis of the servo motor is located as an axis.

Preferably, the cloth cooling shaft cylinder is a hollow cylindrical structure with an open top end and a closed lower end, and the axis of the air supply hole is perpendicular to the axis of the cloth cooling shaft cylinder.

The invention also provides a method for storing the microbial flora, which comprises the following steps:

SS001, pre-placing, before working, opening a sealing door, placing floras to be stored into each bacteria culture tube, after placing the floras, sequentially placing the bacteria culture tubes into placing rotary cylinders in each placing module, after placing, sealing the sealing door, after sealing the sealing door, inflating each clamping bag by an air pump until a monitoring value of an air pressure sensor reaches a set threshold value, after air is fed by the air pump, communicating an oxygen inlet tube with external oxygen feeding equipment, and enabling the oxygen concentration in the tank body to be constant by matching the oxygen inlet tube with an oxygen concentration sensor;

SS002, storage operation, during the storage operation, the refrigerator makes the inner part of the tank body maintain a set low temperature through the cooperation with the temperature probe, during the storage operation, the servo motor works periodically, during the working period of the servo motor, the inner rotary pipe drives the main rotary seat to perform revolution motion, during the revolution motion of the main rotary seat, the outer shaft sleeve drives the upper gear ring to rotate through the transmission gear, after the upper gear ring rotates, the main rotary seat rotates at a set speed while performing revolution motion, during the rotation of the main rotary seat, due to the meshing connection of the driven gear and the lower gear ring, when the placing rotary cylinder rotates on the main rotary seat, synchronous rotation also occurs, through the rotation, each bacteria cultivation tube is uniformly cooled, and during the working period of the servo motor, the electromagnetic ring is periodically electrified, during the electrifying period of the electromagnetic ring, repulsion force is generated between the electromagnetic ring and the permanent magnetic ring, after the repulsion force is generated, so as to drive the excitation cylinder to move upwards, through the electrifying period control of the electromagnetic ring, the up-down vibration frequency of the excitation cylinder can be effectively controlled, through the electrifying period control, the electromagnetic ring can be further opened, and then the vibration of the cultivation tube can be acted on the cultivation tube after the cultivation tube, and the cultivation tube can be taken out, after the cultivation tube can be taken out, and the cultivation tube can be taken out.

(III) advantageous effects

Compared with the prior art, the invention provides a microbial flora storage device and a storage method thereof, and has the following beneficial effects

1. The device can efficiently finish the storage and culture operation of microbial flora by arranging the structures such as the placing module and the refrigerating mechanism, and when the device stores the microbial flora, the static storage structure of the traditional storage device is changed into a dynamic storage structure, a servo motor periodically works during working, an inner rotary pipe drives a main rotary seat to perform revolution motion in the working period of the servo motor, an outer shaft sleeve drives an upper toothed ring to rotate through a transmission gear during the revolution motion of the main rotary seat, after the upper toothed ring rotates, the main rotary seat rotates at a set speed during the revolution motion, and when the main rotary seat rotates, due to the meshing connection of a driven gear and a lower toothed ring, a placing rotary cylinder also synchronously rotates when the main rotary seat rotates, so that each bacteria culture pipe is uniformly cooled through the rotation.

2. According to the invention, through the arrangement of the electromagnetic ring and the permanent magnet ring, an oscillating mechanism for floras is added on the basis of a traditional storage device, when the electromagnetic cultivation device works, the electromagnetic ring is periodically electrified in a working period of a servo motor, repulsion force is generated between the electromagnetic ring and the permanent magnet ring in an electrifying period of the electromagnetic ring, and the repulsion force is generated, so that the excitation cylinder is driven to move upwards, the up-and-down vibration frequency of the excitation cylinder can be effectively controlled through controlling the electrifying period of the electromagnetic ring, the up-and-down displacement stroke of the excitation cylinder can be effectively controlled through controlling the current passing through the electromagnetic ring, after the excitation cylinder vibrates, the excitation cylinder acts on the cultivation tube through the support ring, after the support ring acts on the cultivation tube, the cultivation tube is then excited upwards and downwards, and the floras is uniformly distributed in the cultivation tube through the generation of the up-and-down excitation of the cultivation tube.

Drawings

FIG. 1 is a schematic structural view of a microbial colony storage device according to the present invention;

FIG. 2 is a schematic cross-sectional view of FIG. 1 according to the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 2A;

FIG. 4 is an enlarged view of a portion of FIG. 2 at B according to the present invention;

FIG. 5 is an enlarged view of a portion of the structure of FIG. 2 at C;

FIG. 6 is a schematic structural view of the excitation cylinder and the culture tube of the present invention;

FIG. 7 is an enlarged view of a portion of FIG. 6 at D;

FIG. 8 is a schematic view of the placement module of the present invention mounted on the main rotary base;

FIG. 9 is an enlarged partial schematic view of FIG. 8 at E in accordance with the present invention;

FIG. 10 is a schematic view of the present invention showing the placement of the rotary cylinder and the driven gear;

FIG. 11 is a schematic view of the structure of the main rotary base and the air supply hole of the present invention;

fig. 12 is a schematic structural view of the excitation cylinder of the present invention.

In the figure: 1. a tank body; 2. sealing the door; 3. an outer sleeve; 4. a transmission gear; 5. an inner coil; 6. a servo motor; 7. an air supply hole; 8. a main rotary seat; 9. placing a module; 10. a self-rotating seat; 11. a lower gear ring; 12. an upper gear ring; 13. placing a rotary cylinder; 14. a driven gear; 15. a bacterium cultivation tube; 16. a gas injection clamping pipe; 17. clamping a bag; 18. an air pump; 19. an air guide rotary seat; 20. an electromagnetic ring; 21. an excitation cylinder; 22. a supporting ring; 23. a compression spring; 24. a permanent magnet ring; 25. an air pressure sensor; 26. an air supply branch pipe; 27. a refrigerator; 28. a refrigeration ring cavity; 29. a heat-insulating layer; 30. a refrigeration coil; 31. a main gas supply pipe; 32. an electric control base; 33. a temperature probe; 34. an oxygen concentration sensor; 35. an oxygen inlet pipe; 36. an exhaust pipe; 37. and (4) distributing cold shaft cylinders.

Detailed Description

The invention will be further elucidated and described with reference to a specific embodiment and the drawings of the specification:

referring to fig. 1-12, the present invention: a technical scheme that microbial community storage device adopts is: the tank comprises a tank body 1, wherein a sealing door 2 is arranged at the top of the tank body 1, the sealing door 2 and the tank body 1 are in a hinged state, a buckle matched with the tank body 1 is fixedly arranged on the surface of the sealing door 2, a lockset matched with the buckle is fixedly arranged on the surface of the tank body 1, and a sealing rubber ring matched with the tank body 1 is lined in the inner wall of a sealing plate;

the inner wall of the tank body 1 is rotatably connected with an outer shaft sleeve 3, the peripheral side surface of the outer shaft sleeve 3 is fixedly provided with a transmission gear 4, the inner wall of the outer shaft sleeve 3 is rotatably connected with an inner coil pipe 5, the inner wall of the inner coil pipe 5 is rotatably connected with a cloth cooling shaft cylinder 37, and the port of the refrigeration mechanism is fixedly communicated with the cloth cooling shaft cylinder 37;

the outer shaft sleeve 3 and the inner rotary pipe 5 are both hollow tubular structures with two open ends, and the cooling distribution shaft cylinder 37 is a hollow tubular structure with an open top end and a closed lower end;

the peripheral side surface of the tank body 1 is respectively and fixedly provided with a servo motor 6 and a refrigeration mechanism, and the output shaft end of the servo motor 6 is respectively in transmission connection with the outer shaft sleeve 3 and the inner rotary pipe 5;

driven bevel gears are fixedly arranged on the peripheral side surfaces of the outer shaft sleeve 3 and the inner coil pipe 5, two driving bevel gears are arranged at the output shaft end of the servo motor 6, the peripheral side surfaces of the two driving bevel gears are respectively meshed with the two driven bevel gears, and the two driven bevel gears are symmetrically arranged by taking the horizontal plane where the axis of the servo motor 6 is located as an axis;

the upper parts of the two driven bevel gears are arranged in a distributed state, so that the rotating directions of the outer shaft sleeve 3 and the inner rotating pipe 5 are opposite;

the circumferential side surface of the cloth cooling shaft cylinder 37 is provided with a plurality of groups of air supply holes 7 distributed in a circumferential array, the axis of each air supply hole 7 is vertical to the axis of the cloth cooling shaft cylinder 37, the axis of the cloth cooling shaft cylinder 37 and the axis of the tank body 1 are on the same straight line, and the aperture size of each air supply hole 7 can be customized according to actual requirements;

the peripheral side of the inner coil 5 is fixedly provided with a main rotary seat 8, the peripheral side of the main rotary seat 8 is rotatably connected with the tank body 1, a group of placing modules 9 distributed in a circumferential array is fixedly arranged on the inner wall of the main rotary seat 8, the inner walls of the placing modules 9 are in transmission connection with the transmission gear 4, the bottom of the tank body 1 is fixedly provided with an excitation mechanism matched with the placing modules 9, and the upper part of the tank body 1 is fixedly provided with an auxiliary module.

The placing module 9 comprises a self-rotating seat 10 and a lower gear ring 11 arranged on the bottom surface of the main rotating seat 8 respectively, and the lower gear ring 11 and the main rotating seat 8 are coaxially arranged;

the peripheral side surface of the spinning seat 10 is rotationally connected with the main spinning seat 8, the top surface of the spinning seat 10 is fixedly provided with an upper gear ring 12 which is in transmission connection with the transmission gear 4, the inner wall of the spinning seat 10 is rotationally connected with a group of placing spinning cylinders 13 which are distributed in a circumferential array, and the placing spinning cylinders 13 are hollow cylindrical structures with openings at two ends;

a driven gear 14 is fixedly mounted on the peripheral side surface of each placing rotary cylinder 13, the peripheral side surface of the driven gear 14 is in transmission connection with the lower gear ring 11, a bacterium cultivation pipe 15 with an opening at the top end is clamped on the inner wall of each placing rotary cylinder 13, and the bacterium cultivation pipe 15 is made of glass;

the axial line position of the bottom surface of the self-rotating seat 10 is rotatably connected with a gas injection clamping pipe 16, the peripheral side surface of the gas injection clamping pipe 16 is fixedly communicated with a group of clamping bags 17 distributed in a circumferential array, the peripheral side surface of each clamping bag 17 is matched with a bacterium cultivation pipe 15, and the bottom end of the gas injection clamping pipe 16 is rotatably communicated with a vibration excitation mechanism.

The excitation mechanism comprises an air pump 18, an air guide rotary seat 19, an electromagnetic ring 20 and an excitation cylinder 21 respectively, the air guide rotary seat 19 is of a hollow structure, and a sealing ring attached to the tank body 1 is fixedly arranged on the peripheral side surface of the excitation cylinder 21;

the top surface of the air pump 18 is fixedly connected with the tank body 1, the peripheral side surface of the air guide rotary seat 19 is rotatably connected with the tank body 1, one end of an air outlet of the air pump 18 is rotatably communicated with the air guide rotary seat 19, an air pressure sensor 25 is fixedly arranged at the communication part of the air pump 18 and the air guide rotary seat 19, and the air pressure sensor 25 is used for carrying out auxiliary monitoring on the clamping strength of the clamping bag 17;

when the air pump is used, the air pressure sensor 25 feeds monitored data back to the single chip microcomputer in the electric control seat 32 in real time, and the single chip microcomputer feeds back the data according to the air pressure sensor 25 so as to control the working state of the air pump 18;

the model of the air pressure sensor 25 is QMP 6989;

the air pump 18 is a pumping dual-purpose pump;

the top surface of the air guide rotary seat 19 is rotationally communicated with the air injection clamping tube 16, the bottom end of the cloth cooling shaft barrel 37 is rotationally connected with the air guide rotary seat 19, the inner wall of the excitation barrel 21 and the position corresponding to the bottom of each placing module 9 are fixedly provided with supporting rings 22, the inner wall of the excitation barrel 21 is slidably connected with the air guide rotary seat 19, the inner wall of the excitation barrel 21 is fixedly provided with a guide hole in sliding fit with the air guide rotary seat 19, the cross section of the guide hole is a regular polygon, and the bottom of the air guide rotary seat 19 is fixedly provided with a guide sleeve in shape matched with the guide hole;

through the arrangement of the regular polygon structure of the guide hole, on one hand, the excitation cylinder 21 can slide up and down along the air guide rotary seat 19, and on the other hand, the excitation cylinder 21 and the air guide rotary seat 19 can synchronously rotate;

a group of compression springs 23 distributed in a circumferential array are arranged between the opposite surfaces of the excitation cylinder 21 and the air guide rotary seat 19, a permanent magnet ring 24 matched with the electromagnetic ring 20 is fixedly arranged on the bottom surface of the excitation cylinder 21, the electromagnetic ring 20 is arranged below the permanent magnet ring 24, the bottom surface of the electromagnetic ring 20 is fixedly connected with the tank body 1, and the magnetism of the electromagnetic ring 20 is the same as that of the permanent magnet ring 24;

the permanent magnet ring 24 is a permanent magnet in nature, the electromagnetic ring 20 is an electromagnet in nature, and the electromagnet loses magnetism when being powered on and when being powered off.

The refrigerating mechanism comprises a refrigerator 27 fixedly connected with the tank body 1, a refrigerating annular cavity 28 arranged in the tank body 1, a heat-insulating layer 29 arranged on the outer side of the tank body 1 and a refrigerating coil 30 arranged on the inner wall of the refrigerating annular cavity 28;

the refrigerator 27 is a common device in the prior art and is not described herein, and the refrigerator 27 is configured to supply a cold air source;

the refrigerator 27 is model XH-X266;

one end of the air return opening of the refrigerator 27 is communicated with the air outlet of the refrigeration coil 30, one end of the air outlet of the refrigerator 27 is communicated with the air supply main pipe 31, one end of the air outlet of the air supply main pipe 31 is communicated with the air inlet of the refrigeration coil 30, the top end of the air supply main pipe 31 is fixedly communicated with the air supply branch pipe 26, and the peripheral side surface of the air supply branch pipe 26 is fixedly communicated with the cloth cooling shaft tube 37.

The auxiliary module respectively includes the automatically controlled seat 32 with jar body 1 fixed connection, install temperature probe 33 and the oxygen concentration sensor 34 at jar body 1 top, the inside of automatically controlled seat 32 embeds there is the singlechip, temperature probe 33 and the inside of the equal extension of monitoring end of oxygen concentration sensor 34 to jar body 1, temperature probe 33 and the data end of oxygen concentration sensor 34 all are connected with the singlechip electricity, jar body 1 top fixed mounting has oxygen inlet pipe 35 and blast pipe 36 respectively, the inside of oxygen inlet pipe 35 and blast pipe 36 all installs the pneumatic valve.

The model of the temperature probe 33 is DS18B20;

the model of the oxygen concentration sensor 34 is OOM102-1;

the type of the singlechip is FX1N-60MR-001;

a method of storing a microbial flora comprising the steps of:

SS001, pre-placing, before working, opening the sealing door 2, placing the floras to be stored into each bacteria cultivation pipe 15, after placing the floras, sequentially placing the bacteria cultivation pipes 15 into the placing rotary cylinders 13 in the placing modules 9, after placing, sealing the sealing door 2, after sealing the sealing door 2, inflating each clamping bag 17 by the air pump 18 until the monitoring value of the air pressure sensor 25 reaches a set threshold value, after air is fed by the air pump 18, communicating the oxygen inlet pipe 35 with external oxygen feeding equipment, and keeping the oxygen concentration in the tank body 1 constant by the matching of the oxygen inlet pipe 35 and the oxygen concentration sensor 34;

SS002, storage operation, during which the refrigerator 27 maintains the interior of the tank body 1 at a set low temperature through cooperation with the temperature probe 33, and during which the servo motor 6 periodically operates, the inner rotary tube 5 drives the main rotary base 8 to perform revolution motion within the operating period of the servo motor 6, during the revolution motion of the main rotary base 8, the outer sleeve 3 drives the upper gear ring 12 to rotate through the transmission gear 4, after the upper gear ring 12 rotates, the main rotary base 8 simultaneously performs rotation at a set speed during the revolution motion, when the main rotary base 8 performs rotation, the placing rotary cylinder 13 also performs synchronous rotation when the placing rotary cylinder 8 performs rotation at the main rotary base 8 due to the meshing connection of the driven gear 14 and the lower gear ring 11, thereby enabling each of the culture tubes 15 to be uniformly cooled through the rotation, in the working period of the servo motor 6, the electromagnetic ring 20 is periodically electrified, in the electrifying period of the electromagnetic ring 20, repulsive force is generated between the electromagnetic ring 20 and the permanent magnetic ring 24, after the repulsive force is generated, the excitation cylinder 21 is driven to move upwards, the up-and-down vibration frequency of the excitation cylinder 21 can be effectively controlled through the electrifying period control of the electromagnetic ring 20, the up-and-down displacement stroke of the excitation cylinder 21 can be effectively controlled through the current control of the electromagnetic ring 20, after the excitation cylinder 21 vibrates, the vibration cylinder then acts on the culture tube 15 through the supporting ring 22, after the supporting ring 22 acts on the culture tube 15, the culture tube 15 is then excited up and down, and when the culture tube 15 needs to be taken out, the sealing door 2 is opened, and then the taking-out operation of the storage culture tube 15 can be completed.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The utility model provides a microbial community storage device, includes jar body (1), a door (2), its characterized in that are installed to the top of jar body (1): the inner wall of the jar body (1) rotates and is connected with outer axle sleeve (3), all side fixed mounting of outer axle sleeve (3) has drive gear (4), the inner wall of outer axle sleeve (3) rotates and is connected with interior screwed pipe (5), the inner wall of interior screwed pipe (5) rotates and is connected with cloth cold beam barrel (37), all side fixed mounting of the jar body (1) has servo motor (6) and refrigeration mechanism respectively, the output shaft end of servo motor (6) is connected with outer axle sleeve (3) and interior screwed pipe (5) transmission respectively, the port and the cloth cold beam barrel (37) fixed intercommunication of refrigeration mechanism, all side of cloth cold beam barrel (37) is seted up the blast hole (7) that a plurality of groups are circumference array distribution, all side of interior screwed pipe (5) is equipped with main seat (8) admittedly, the main side of seat (8) of revolving of seat (8) rotates with the jar body (1) and is connected, the main inner wall fixed mounting of revolving seat (8) has the module (9) of placing that are a set of circumference array distribution, a set of placing the inner wall of placing module (9) all with the internal wall of the jar body (4) the drive gear is connected the excitation module (1) and the cooperation of the fixed mounting of the drive gear (1) is connected with the bottom of the auxiliary mechanism (1).

2. A microbial community storage device according to claim 1, wherein: place module (9) and include respectively revolve seat (10) and install in the owner and revolve lower ring gear (11) of seat (8) bottom surface soon, the side of week of seat (10) revolves seat (8) with the owner and rotates and be connected, the top surface fixed mounting who revolves seat (10) has last ring gear (12) of being connected with drive gear (4) transmission, the inner wall of seat (10) of revolving rotates and is connected with a set of placing that is circumference array distribution revolves a section of thick bamboo (13), every place the equal fixed mounting of side of week of revolving a section of thick bamboo (13) has driven gear (14), the side of week of driven gear (14) is connected with lower ring gear (11) transmission, the inner wall joint of placing revolving a section of thick bamboo (13) has top open-ended to bank up fungus pipe (15), the bottom surface axis position of week of seat (10) of revolving is connected with gas injection clamp pipe (16), the fixed intercommunication of side of gas injection clamp pipe (16) has a set of being circumference array distribution and presss from both sides bag (17), every press from both sides the side of bag (17) all with fungus pipe (15) of week, the cooperation of gas injection clamp mechanism (16) and the excitation.

3. The microbial colony storage device according to claim 1, wherein: the excitation mechanism includes air pump (18), air guide revolves seat (19), electromagnetism ring (20) and excitation section of thick bamboo (21) respectively, the top surface and the jar body (1) fixed connection of air pump (18), the week side of air guide revolves seat (19) rotates with jar body (1) to be connected, the one end and the air guide of air pump (18) gas outlet revolve seat (19) and rotate the intercommunication, the top surface and the gas injection clamp tube (16) of air guide revolve seat (19) rotate the intercommunication, the bottom and the air guide of cloth cold axis section of thick bamboo (37) revolve seat (19) and rotate to be connected, the inner wall of excitation section of thick bamboo (21) just corresponds every equal fixed mounting in position of placing module (9) bottom has backing ring (22), the inner wall and the air guide of excitation section of thick bamboo (21) revolve seat (19) sliding connection, install a set of resistance to compression spring (23) that are circumference array distribution between the relative surface of excitation section of thick bamboo (21) and air guide revolve seat (19), the fixed mounting of excitation section of thick bamboo bottom surface (21) have with electromagnetism ring (20) permanent magnetism ring (24), set up in jar body (20) below fixed connection.

4. A microbial community storage device according to claim 3, wherein: air pump (18) revolves the fixed baroceptor (25) that is provided with in intercommunication department of seat (19) with the air guide, the air guide revolves seat (19) and is hollow structure, the all sides fixed mounting of cylinder (21) that shakes has the sealing washer of laminating with jar body (1), the inner wall of cylinder (21) that shakes is fixed set up revolve seat (19) sliding fit's guiding hole with the air guide, the cross section of guiding hole is regular polygon, the air guide revolves the fixed guide pin bushing that is provided with guiding hole shape adaptation in bottom of seat (19).

5. A microbial community storage device according to claim 3, wherein: the magnetism of the electromagnetic ring (20) is the same as that of the permanent magnet ring (24).

6. A microbial community storage device according to claim 1, wherein: refrigerating mechanism include with jar body (1) fixed connection's refrigerator (27), set up in jar internal portion of body (1) refrigeration ring chamber (28), set up in heat preservation (29) in the jar body (1) outside and install in refrigeration coil (30) of refrigeration ring chamber (28) inner wall, the one end of refrigerator (27) return air mouth communicates with the gas outlet of refrigeration coil (30), the one end intercommunication of refrigerator (27) gas outlet has the person in charge of supplying air (31), the one end of the person in charge of supplying air (31) gas outlet communicates with the air inlet of refrigeration coil (30), the fixed intercommunication in top of the person in charge of supplying air (31) has the branch pipe of supplying air (26), the week side and the fixed intercommunication of cloth cold shaft section of thick bamboo (37) of branch pipe (26).

7. A microbial community storage device according to claim 1, wherein: the utility model discloses a solar water heater, including the jar body (1) and the oxygen concentration sensor (34), supplementary module includes respectively with jar body (1) fixed connection's automatically controlled seat (32), install in the temperature probe (33) and the oxygen concentration sensor (34) at jar body (1) top, the inside of automatically controlled seat (32) is built-in to have the singlechip, the monitoring end of temperature probe (33) and oxygen concentration sensor (34) all extends to the inside of jar body (1), the data end of temperature probe (33) and oxygen concentration sensor (34) all is connected with the monolithic is electromechanical, the top of jar body (1) is fixed mounting respectively has into oxygen pipe (35) and blast pipe (36), the pneumatic valve is all installed to the inside of entering oxygen pipe (35) and blast pipe (36).

8. The microbial colony storage device according to claim 1, wherein: outer axle sleeve (3) and interior screwed pipe (5) are both ends open-ended cavity tubular structure, the equal fixed mounting in all sides of outer axle sleeve (3) and interior screwed pipe (5) has driven bevel gear, two drive bevel gear, two are installed to the output axle head of servo motor (6) the all sides of drive bevel gear mesh with two driven bevel gear respectively, two driven bevel gear uses the axis place horizontal plane of servo motor (6) to be the symmetry setting for the axle.

9. A microbial community storage device according to claim 1, wherein: the cloth cooling shaft cylinder (37) is of a hollow cylindrical structure with an open top end and a closed lower end, and the axis of the air supply hole (7) is perpendicular to the axis of the cloth cooling shaft cylinder (37).

10. A method for storing a microbial flora, which comprises using a microbial flora storage device as claimed in any of claims 1 to 9, wherein: the method comprises the following steps:

SS001, pre-placing, before working, opening a sealing door (2), placing floras to be stored into each bacteria culture tube (15), placing the floras, sequentially placing the bacteria culture tubes (15) into placing rotary drums (13) in each placing module (9), after placing, sealing the sealing door (2), after sealing the sealing door (2), inflating an air pump (18) into each clamping bag (17) until a monitoring value of an air pressure sensor (25) reaches a set threshold value, after air is fed into the air pump (18), communicating an oxygen inlet pipe (35) with external oxygen feeding equipment, and enabling the oxygen concentration in the tank body (1) to be constant through the matching of the oxygen inlet pipe (35) and an oxygen concentration sensor (34);

SS002, storage operation, during the storage operation, the refrigerator (27) maintains the interior of the tank body (1) at a set low temperature through the matching with the temperature probe (33), and during the storage operation, the servo motor (6) periodically works, in the working period of the servo motor (6), the inner rotary pipe (5) drives the main rotary base (8) to perform revolution movement, during the revolution movement of the main rotary base (8), the outer shaft sleeve (3) drives the upper gear ring (12) to rotate through the transmission gear (4), after the upper gear ring (12) rotates, the main rotary base (8) simultaneously performs rotation at a set speed during the revolution movement, when the main rotary base (8) performs rotation, because the driven gear (14) is connected with the lower gear ring (11) in an engaged manner, the rotary cylinder (13) is placed to perform rotation on the main rotary base (8), synchronous rotation also occurs, thereby each bacteria culture pipe (15) is uniformly cooled, in the working period of the servo motor (6), the electromagnetic ring (20) generates a periodic repulsive force, after the electromagnetic ring (20) is electrified and the electromagnetic ring (20) controls the electromagnetic ring (21) to generate an upward electromagnetic ring vibration frequency, after the electromagnetic ring (21) is electrified and the electromagnetic ring (21) is controlled by an effective electromagnetic ring vibration frequency, the vertical displacement stroke of the excitation cylinder (21) can be effectively controlled, after the excitation cylinder (21) vibrates, the excitation cylinder acts on the culture tube (15) through the supporting ring (22), after the supporting ring (22) acts on the culture tube (15), the culture tube (15) is excited vertically, and when the culture tube (15) needs to be taken out, the sealing door (2) is opened, so that the operation of taking out the storage culture tube (15) can be completed.