CN112029650A - Fermentation equipment for lactobacillus plantarum and working method - Google Patents
Fermentation equipment for lactobacillus plantarum and working method Download PDFInfo
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- CN112029650A CN112029650A CN202010487947.5A CN202010487947A CN112029650A CN 112029650 A CN112029650 A CN 112029650A CN 202010487947 A CN202010487947 A CN 202010487947A CN 112029650 A CN112029650 A CN 112029650A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 150
- 230000004151 fermentation Effects 0.000 title claims abstract description 150
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 51
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 51
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 109
- 238000010438 heat treatment Methods 0.000 claims abstract description 86
- 241000186660 Lactobacillus Species 0.000 claims abstract description 8
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims description 78
- 239000000463 material Substances 0.000 claims description 24
- 238000005507 spraying Methods 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 21
- 239000000498 cooling water Substances 0.000 claims description 15
- 238000004140 cleaning Methods 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000000523 sample Substances 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000007858 starting material Substances 0.000 claims description 8
- 238000005057 refrigeration Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000012423 maintenance Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- BGOFCVIGEYGEOF-UJPOAAIJSA-N helicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1C=O BGOFCVIGEYGEOF-UJPOAAIJSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention discloses a fermentation device and a working method of lactobacillus plantarum, relates to the technical field of fermentation of lactobacillus, and aims to solve the problem that the conventional fermentation device of lactobacillus plantarum only has the function of standing fermentation and has single function, so that the fermentation time is long in winter. The supporting legs is installed to the below of fermentation cylinder main part, and the supporting legs is provided with threely, and three supporting legs all with fermentation cylinder main part welded connection, the outside fixed mounting of fermentation cylinder main part has water proof heating jacket, the upper end of water proof heating jacket one side is provided with the water intaking valve, the lower extreme of water proof heating jacket one side is provided with the outlet valve, the inside of water proof heating jacket is provided with water bath heating coil, and the inner wall fixed connection of water bath heating coil and water proof heating jacket, the equal fixed mounting in both ends of water bath heating coil has the wiring end, and the wiring end runs through and extends to water proof heating jacket.
Description
Technical Field
The invention relates to the technical field of fermentation of lactobacillus, in particular to fermentation equipment and a working method of lactobacillus plantarum.
Background
The lactobacillus plantarum is one of lactobacillus, has the optimal growth temperature of 30-35, is anaerobic or facultative anaerobic, has a straight or bent rod shape, is single or sometimes paired or chained, has the optimal pH value of about 6.5, and belongs to homofermentation lactobacillus. The bacterium is different from other lactic acid bacteria in that the number of viable bacteria of the bacterium is higher, a large amount of acid can be produced, the PH value in water is stable and not increased, and the produced acidic substances can degrade heavy metals; since this bacterium is an anaerobic bacterium, it produces a characteristic lactobacillin, a biological preservative, during the propagation process. In the middle and later period of cultivation, as the excrement and the residual feed of animals are increased, the animals sink to the bottom of the pond and are rotten, a lot of germs are bred, a large amount of ammonia nitrogen and nitrite are generated, and the phenomenon that the bottom is killed by theft is serious. If the lactobacillus plantarum is used for a long time, the rot of bottom excrement and residual feed can be well inhibited, the increase of ammonia nitrogen and nitrite is reduced, the consumption of chemical degradation elements is greatly reduced, and the breeding cost is reduced.
However, the existing lactobacillus plantarum fermentation equipment only can play a role in standing fermentation, and has a single function, so that the fermentation time in winter is long; therefore, the existing requirements are not met, and a fermentation device and a working method of the lactobacillus plantarum are provided for the requirement.
Disclosure of Invention
The invention aims to provide fermentation equipment of lactobacillus plantarum and a working method thereof, and aims to solve the problem that the conventional fermentation equipment of lactobacillus plantarum in the background art only has the function of standing fermentation and single function, so that the fermentation time is long in winter.
In order to achieve the purpose, the invention provides the following technical scheme: a fermentation device of lactobacillus plantarum comprises a fermentation tank main body, wherein three supporting legs are arranged below the fermentation tank main body and are welded with the fermentation tank main body, a water-proof heating jacket is fixedly arranged outside the fermentation tank main body, a water inlet valve is arranged at the upper end of one side of the water-proof heating jacket, a water outlet valve is arranged at the lower end of one side of the water-proof heating jacket, a water bath heating coil is arranged inside the water-proof heating jacket and is fixedly connected with the inner wall of the water-proof heating jacket, wiring ends are fixedly arranged at the two ends of the water bath heating coil and penetrate through and extend to the water-proof heating jacket, the connection part of the wiring ends and the water-proof heating jacket is connected in a sealing manner, a sealing cover is arranged at the upper end of the fermentation tank main body, and an internal thread mechanism is arranged on the inner wall of the lower end of the sealing cover, the inner thread mechanism and the sealing cover are of an integral structure, the outer wall of the upper end of the fermentation tank main body is provided with an outer thread mechanism, the outer thread mechanism and the fermentation tank main body are of an integral structure, the inside of the sealing cover is provided with an annular spraying pipe, the outer wall of the annular spraying pipe is welded with the sealing cover, the annular spraying pipe is fixedly connected with a water inlet joint, one end of the water inlet joint penetrates through and extends to the outside of the sealing cover, one end of the water inlet joint is in threaded connection with a sealing head, the lower end of the sealing cover is fixedly provided with a nozzle, twelve nozzles are arranged on the nozzle, the upper end of the sealing cover is provided with a servo motor, the lower surface of the servo motor is fixedly provided with a motor bracket, the servo motor is fixedly connected with the sealing cover through the motor bracket, the output end of the servo motor is fixedly provided with a stirring shaft, and, (mixing) shaft and the junction fixed mounting of closing cap have mechanical seal, the one end fixed mounting of (mixing) shaft has the weight, one side of servo motor is provided with the feed inlet, and feed inlet and closing cap fixed connection, servo motor's opposite side is provided with the thermometer, and thermometer and closing cap threaded connection, the lower extreme fixed mounting of thermometer has the probe, and the one end of probe runs through the inside of closing cap and extending to the fermentation cylinder main part, one side fixed mounting of fermentation cylinder main part has the admission valve, and the inner tube of admission valve extends to the bottom of fermentation cylinder main part, the opposite side fixed mounting of fermentation cylinder main part has discharge valve.
Preferably, the sealing cover is in threaded connection with an external thread mechanism at the upper end of the fermentation tank body through an internal thread mechanism at the lower end.
Preferably, the joint of the sealing cover and the fermentation tank main body is provided with a rubber sealing ring, and the rubber sealing ring is in interference fit with the fermentation tank main body.
Preferably, the outer wall of the stirring shaft is provided with five helical blades, and the helical blades are in threaded connection with the stirring shaft through screws.
Preferably, the two sides of the outer wall of the sealing cover are both provided with rotary hanging lugs, and the rotary hanging lugs are connected with the sealing cover in a welded mode.
Preferably, the feed inlet and the discharge outlet are internally provided with valves, and the valves are hermetically connected with the feed inlet and the discharge outlet.
Preferably, the working method of the lactobacillus plantarum fermentation equipment comprises the following steps:
the method comprises the following steps: preparing a lactobacillus plantarum starter, namely inoculating lactobacillus plantarum strains to an MRS agar plate, standing for two days at 35 ℃ to activate the strains, inoculating the activated lactobacillus plantarum into a liquid culture medium, standing for one day at 35 ℃ to obtain a seed solution, inoculating the seed solution into a propagation culture medium, and continuously standing for one day at 35 ℃ to obtain the lactobacillus plantarum starter;
step two: the material that will need the fermentation is poured into the fermentation cylinder main part from feed inlet department in, according to material and plant lactobacillus leaven 200: 1, adding a proper amount of lactobacillus plantarum ferment into the tank, and starting a servo motor to drive a stirring shaft and helical blades on an output end to rotate so as to fully mix the material and the lactobacillus plantarum ferment;
step three: an air inlet valve at one side of the fermentation tank main body is communicated with an air compressor, one end of the air compressor is connected with a sterile air tank, and the air compressor is also arranged at an exhaust valve at the other side of the fermentation tank main body, so that bacteria-containing air in the tank is replaced by sterile air to ensure the fermentation quality of the material;
step four: after the tightness of each part of the fermentation tank main body is checked, the fermentation tank main body is allowed to stand at normal temperature for two to three days;
step five: and opening a valve at the discharge port, discharging the fermented materials, and cleaning the tank body to quickly enter the next fermentation operation.
Preferably, in the fourth step, if the temperature is too high in summer, the tank body can be cooled by injecting cooling water into the water-proof heating jacket, the heat exchange area is the diameter of the fermentation tank x 3.14 x the height of the water-proof heating jacket, a water inlet and a water outlet of the cooling water need to be communicated with a water circulation water cooler to maintain the refrigeration temperature, the temperature in the tank is controlled to be 30-35 ℃, and the tank is kept still for two to three days to complete the fermentation work; and in winter, clear water is required to be injected into the waterproof heating jacket, the water is heated by the water bath heating coil in the waterproof heating jacket, so that the water passes through the early heating stage, cooling water is introduced into the jacket after heat is generated in the thallus growth stage, the temperature is kept constant by continuously cooling by virtue of an external circulating water cooler, and the temperature is controlled to be 30-35 ℃, so that the time required by fermentation in winter is effectively shortened.
Preferably, in the fifth step, when the fermentation tank is cleaned, the water inlet joint at the upper end of the sealing cover can be communicated with the water pump, the water pump is used for conveying water into the annular spraying pipe at the lower end of the sealing cover, and the nozzle at the lower end of the annular spraying pipe is used for cleaning the interior of the tank body, so that the cleaning operation is convenient and fast.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, a water-proof heating jacket is arranged outside the equipment, a water bath heating coil is arranged inside the water-proof heating jacket, the water bath heating coil is not directly contacted with the equipment shell but fixed with the inner wall of the water-proof heating jacket, if the temperature is too high in summer, the tank body can be cooled by injecting cooling water into the water-proof heating jacket, the heat exchange area is the diameter of a fermentation tank and 3.14 and the height of the water-proof heating jacket, and a cooling water inlet and outlet need to be communicated with a water circulation water cooler to maintain the refrigeration temperature, so that the temperature in the tank is controlled to be 30-35 ℃, and the tank is kept still for two to three days to complete the fermentation work; and in winter, clear water is required to be injected into the waterproof heating jacket, the water is heated by the water bath heating coil in the waterproof heating jacket, so that the water passes through the early heating stage, cooling water is introduced into the jacket after heat is generated in the thallus growth stage, the temperature is kept constant by continuously cooling by virtue of an external circulating water cooler, and the temperature is controlled to be 30-35 ℃, so that the time required by fermentation in winter is effectively shortened.
2. Through the inside fixed mounting annular shower at the closing cap, treat that the fermentation tank unloads the back that finishes, can communicate water supply connector and the water pump of closing cap upper end, carry water to the annular shower of closing cap lower extreme in by the water pump, utilize the nozzle of annular shower lower extreme to accomplish the inside cleaning work of jar, convenient and fast makes it can get into the fermentation work of next time fast.
3. Through set up female screw mechanism on the inner wall at the closing cap lower extreme, set up external screw mechanism on the outer wall of fermentation cylinder main part upper end, make both can threaded connection, easy dismounting is swift, the rotatory hangers of closing cap both sides can help the staff to assist rotatory closing cap part, make it dismouting smoothly, because of servo motor, the equal fixed mounting of thermometer and annular shower is on the closing cap, so this mounting structure can effectively improve equipment's maintenance efficiency, rubber seal is installed with the junction of fermentation cylinder main part to the closing cap, outside air enters into jar internal portion from the junction during can preventing the fermentation.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the water-proof heating jacket according to the present invention;
FIG. 3 is a schematic view of the internal structure of the fermenter body according to the present invention;
FIG. 4 is a schematic view of the structure of the lower end face of the closure of the present invention;
FIG. 5 is an enlarged view of a portion of the present invention at A;
FIG. 6 is a schematic diagram of the working method of Lactobacillus plantarum fermentation according to the present invention;
in the figure: 1. a fermenter main body; 2. a water-proof heating jacket; 3. a water inlet valve; 4. a water outlet valve; 5. a terminal; 6. sealing the cover; 7. a rubber seal ring; 8. rotating the hangers; 9. an intake valve; 10. an exhaust valve; 11. supporting legs; 12. a discharge opening; 13. a feed inlet; 14. a valve; 15. a servo motor; 16. a motor bracket; 17. a thermometer; 18. a water bath heating coil; 19. a stirring shaft; 20. a mechanical seal; 21. a probe; 22. a water inlet joint; 23. an annular shower pipe; 24. a nozzle; 25. a helical blade; 26. a screw; 27. a weighting block; 28. an internal thread mechanism; 29. and the external thread mechanism.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1:
referring to fig. 1-6, an embodiment of the present invention is shown: a fermentation device of lactobacillus plantarum comprises a fermentation tank main body 1, wherein supporting legs 11 are arranged below the fermentation tank main body 1, the number of the supporting legs 11 is three, the three supporting legs 11 are all welded with the fermentation tank main body 1, a water-proof heating jacket 2 is fixedly arranged outside the fermentation tank main body 1, a water inlet valve 3 is arranged at the upper end of one side of the water-proof heating jacket 2, a water outlet valve 4 is arranged at the lower end of one side of the water-proof heating jacket 2, a water bath heating coil 18 is arranged inside the water-proof heating jacket 2, the water bath heating coil 18 is fixedly connected with the inner wall of the water-proof heating jacket 2, wiring terminals 5 are fixedly arranged at the two ends of the water bath heating coil 18, the wiring terminals 5 penetrate through and extend to the water-proof heating jacket 2, the connection part of the wiring terminals 5 and the water-proof heating jacket 2 is in sealing connection, a sealing cover 6 is arranged at the upper end, the internal thread mechanism 28 and the sealing cover 6 are integrated, the external thread mechanism 29 is arranged on the outer wall of the upper end of the fermentation tank main body 1, the external thread mechanism 29 and the fermentation tank main body 1 are integrated, the annular spraying pipe 23 is arranged inside the sealing cover 6, the outer wall of the annular spraying pipe 23 is welded with the sealing cover 6, the annular spraying pipe 23 is fixedly connected with the water inlet connector 22, one end of the water inlet connector 22 penetrates through and extends to the outside of the sealing cover 6, one end of the water inlet connector 22 is connected with the sealing head in a threaded manner, the lower end of the sealing cover 6 is fixedly provided with the nozzles 24, twelve nozzles 24 are arranged on the nozzles 24, the upper end of the sealing cover 6 is provided with the servo motor 15, the lower surface of the servo motor 15 is fixedly provided with the motor support 16, the servo motor 15 is fixedly connected with the sealing cover 6 through the motor support 16, the output end of the servo motor 15 is fixedly provided with the stirring shaft 19, mechanical seal 20 is fixedly mounted at the joint of stirring shaft 19 and sealing cover 6, a weighting block 27 is fixedly mounted at one end of stirring shaft 19, a feed inlet 13 is arranged at one side of servo motor 15, feed inlet 13 is fixedly connected with sealing cover 6, a thermometer 17 is arranged at the other side of servo motor 15, thermometer 17 is in threaded connection with sealing cover 6, a probe 21 is fixedly mounted at the lower end of thermometer 17, one end of probe 21 penetrates through sealing cover 6 and extends to the inside of fermentation tank body 1, an air inlet valve 9 is fixedly mounted at one side of fermentation tank body 1, the inner tube of air inlet valve 9 extends to the bottom of fermentation tank body 1, and an exhaust valve 10 is fixedly mounted at the other side of fermentation tank body 1.
Further, the closing cap 6 is in threaded connection with the external thread mechanism 29 at the upper end of the fermentation tank body 1 through the internal thread mechanism 28 at the lower end, the closing cap 6 and the fermentation tank body 1 are fixedly installed in a threaded connection mode, the assembly and disassembly are convenient, and the maintenance efficiency of the device can be effectively improved due to the fact that the servo motor 15, the thermometer 17 and the annular spraying pipe 23 are fixedly installed on the closing cap 6.
Further, the junction of closing cap 6 and fermentation cylinder main part 1 is provided with rubber seal 7, and rubber seal 7 and fermentation cylinder main part 1 interference fit, and rubber seal 7 can strengthen the leakproofness of closing cap 6 and fermentation cylinder main part 1 junction, avoids outside air to enter into jar internal portion.
Further, be provided with helical blade 25 on the outer wall of (mixing) shaft 19, helical blade 25 passes through screw 26 and (mixing) shaft 19 threaded connection, and screw 26 is provided with five, and spiral helicine blade is big with material area of contact, can effectively improve the mixing efficiency of material and plant lactobacillus leaven.
Further, the outer wall both sides of closing cap 6 all are provided with rotatory hangers 8, and rotatory hangers 8 and closing cap 6 welded connection, and rotatory hangers 8 can help equipment to accomplish handling work on the one hand, and on the other hand can help convenient separation closing cap 6 of staff and fermentation cylinder main part 1.
Further, valve 14 is all installed to the inside of feed inlet 13 and discharge opening 12, and valve 14 and feed inlet 13 and discharge opening 12 sealing connection, and valve 14 can control the business turn over of material, can play sealing device's effect when closed.
Further, a working method of fermentation equipment for lactobacillus plantarum comprises the following steps:
the method comprises the following steps: preparing a lactobacillus plantarum starter, namely inoculating lactobacillus plantarum strains to an MRS agar plate, standing for two days at 35 ℃ to activate the strains, inoculating the activated lactobacillus plantarum into a liquid culture medium, standing for one day at 35 ℃ to obtain a seed solution, inoculating the seed solution into a propagation culture medium, and continuously standing for one day at 35 ℃ to obtain the lactobacillus plantarum starter;
step two: pouring 500kg of material and 2.5kg of lactobacillus plantarum ferment into the fermentation tank main body 1 from the feeding hole 13, starting the servo motor 15 to drive the stirring shaft 19 and the helical blade 25 on the output end to rotate, so that the material and the lactobacillus plantarum ferment can be fully mixed;
step three: an air inlet valve 9 at one side of the fermentation tank main body 1 is communicated with an air compressor, one end of the air compressor is connected with a sterile air tank, and the air compressor is also installed at an air outlet valve 10 at the other side of the fermentation tank main body 1, so that the bacteria-containing air in the tank is replaced by sterile air to ensure the fermentation quality of the material;
step four: after checking the sealing performance of each part of the fermentation tank main body 1, standing and fermenting for two days at normal temperature;
step five: and opening a valve at the discharge opening 12 to discharge the fermented materials, and cleaning the tank body to quickly enter the next fermentation work.
Further, in the fourth step, if the temperature is too high in summer, the tank body can be cooled by injecting cooling water into the water-proof heating jacket 2, the heat exchange area is the diameter of the fermentation tank, 3.14 and the height of the water-proof heating jacket 2, a water inlet and a water outlet of the cooling water need to be communicated with a water circulation water cooler to maintain the refrigeration temperature, the temperature in the tank is controlled to be 35 ℃, and the tank is kept still for two days to complete the fermentation work; and when in winter, clear water needs to be injected into the waterproof heating jacket 2, the water is heated by the water bath heating coil 18 in the waterproof heating jacket 2, so that the water passes through the early heating stage, after the thalli grow stage generates heat, cooling water is introduced into the jacket, the temperature is continuously reduced by virtue of the external circulating water cooler, so that the jacket is in a constant temperature state, and the temperature is controlled at 35 ℃, thereby effectively shortening the time required by fermentation in winter.
Further, in the fifth step, when the fermentation tank is cleaned, the water inlet connector 22 at the upper end of the sealing cover 6 can be communicated with a water pump, the water pump is used for conveying water into the annular spraying pipe 23 at the lower end of the sealing cover 6, and the nozzle 24 at the lower end of the annular spraying pipe 23 is used for cleaning the interior of the tank body, so that the cleaning operation is convenient and fast.
Example 2:
referring to fig. 1-6, an embodiment of the present invention is shown: a fermentation device of lactobacillus plantarum comprises a fermentation tank main body 1, wherein supporting legs 11 are arranged below the fermentation tank main body 1, the number of the supporting legs 11 is three, the three supporting legs 11 are all welded with the fermentation tank main body 1, a water-proof heating jacket 2 is fixedly arranged outside the fermentation tank main body 1, a water inlet valve 3 is arranged at the upper end of one side of the water-proof heating jacket 2, a water outlet valve 4 is arranged at the lower end of one side of the water-proof heating jacket 2, a water bath heating coil 18 is arranged inside the water-proof heating jacket 2, the water bath heating coil 18 is fixedly connected with the inner wall of the water-proof heating jacket 2, wiring terminals 5 are fixedly arranged at the two ends of the water bath heating coil 18, the wiring terminals 5 penetrate through and extend to the water-proof heating jacket 2, the connection part of the wiring terminals 5 and the water-proof heating jacket 2 is in sealing connection, a sealing cover 6 is arranged at the upper end, the internal thread mechanism 28 and the sealing cover 6 are integrated, the external thread mechanism 29 is arranged on the outer wall of the upper end of the fermentation tank main body 1, the external thread mechanism 29 and the fermentation tank main body 1 are integrated, the annular spraying pipe 23 is arranged inside the sealing cover 6, the outer wall of the annular spraying pipe 23 is welded with the sealing cover 6, the annular spraying pipe 23 is fixedly connected with the water inlet connector 22, one end of the water inlet connector 22 penetrates through and extends to the outside of the sealing cover 6, one end of the water inlet connector 22 is connected with the sealing head in a threaded manner, the lower end of the sealing cover 6 is fixedly provided with the nozzles 24, twelve nozzles 24 are arranged on the nozzles 24, the upper end of the sealing cover 6 is provided with the servo motor 15, the lower surface of the servo motor 15 is fixedly provided with the motor support 16, the servo motor 15 is fixedly connected with the sealing cover 6 through the motor support 16, the output end of the servo motor 15 is fixedly provided with the stirring shaft 19, mechanical seal 20 is fixedly mounted at the joint of stirring shaft 19 and sealing cover 6, a weighting block 27 is fixedly mounted at one end of stirring shaft 19, a feed inlet 13 is arranged at one side of servo motor 15, feed inlet 13 is fixedly connected with sealing cover 6, a thermometer 17 is arranged at the other side of servo motor 15, thermometer 17 is in threaded connection with sealing cover 6, a probe 21 is fixedly mounted at the lower end of thermometer 17, one end of probe 21 penetrates through sealing cover 6 and extends to the inside of fermentation tank body 1, an air inlet valve 9 is fixedly mounted at one side of fermentation tank body 1, the inner tube of air inlet valve 9 extends to the bottom of fermentation tank body 1, and an exhaust valve 10 is fixedly mounted at the other side of fermentation tank body 1.
Further, the closing cap 6 is in threaded connection with the external thread mechanism 29 at the upper end of the fermentation tank body 1 through the internal thread mechanism 28 at the lower end, the closing cap 6 and the fermentation tank body 1 are fixedly installed in a threaded connection mode, the assembly and disassembly are convenient, and the maintenance efficiency of the device can be effectively improved due to the fact that the servo motor 15, the thermometer 17 and the annular spraying pipe 23 are fixedly installed on the closing cap 6.
Further, the junction of closing cap 6 and fermentation cylinder main part 1 is provided with rubber seal 7, and rubber seal 7 and fermentation cylinder main part 1 interference fit, and rubber seal 7 can strengthen the leakproofness of closing cap 6 and fermentation cylinder main part 1 junction, avoids outside air to enter into jar internal portion.
Further, be provided with helical blade 25 on the outer wall of (mixing) shaft 19, helical blade 25 passes through screw 26 and (mixing) shaft 19 threaded connection, and screw 26 is provided with five, and spiral helicine blade is big with material area of contact, can effectively improve the mixing efficiency of material and plant lactobacillus leaven.
Further, the outer wall both sides of closing cap 6 all are provided with rotatory hangers 8, and rotatory hangers 8 and closing cap 6 welded connection, and rotatory hangers 8 can help equipment to accomplish handling work on the one hand, and on the other hand can help convenient separation closing cap 6 of staff and fermentation cylinder main part 1.
Further, valve 14 is all installed to the inside of feed inlet 13 and discharge opening 12, and valve 14 and feed inlet 13 and discharge opening 12 sealing connection, and valve 14 can control the business turn over of material, can play sealing device's effect when closed.
Further, a working method of fermentation equipment for lactobacillus plantarum comprises the following steps:
the method comprises the following steps: preparing a lactobacillus plantarum starter, namely inoculating lactobacillus plantarum strains to an MRS agar plate, standing for two days at 35 ℃ to activate the strains, inoculating the activated lactobacillus plantarum into a liquid culture medium, standing for one day at 35 ℃ to obtain a seed solution, inoculating the seed solution into a propagation culture medium, and continuously standing for one day at 35 ℃ to obtain the lactobacillus plantarum starter;
step two: pouring 1000kg of material and 5kg of lactobacillus plantarum ferment into the fermentation tank main body 1 from the feeding hole 13, starting the servo motor 15 to drive the stirring shaft 19 and the helical blades 25 on the output end to rotate, so that the material and the lactobacillus plantarum ferment can be fully mixed;
step three: an air inlet valve 9 at one side of the fermentation tank main body 1 is communicated with an air compressor, one end of the air compressor is connected with a sterile air tank, and the air compressor is also installed at an air outlet valve 10 at the other side of the fermentation tank main body 1, so that the bacteria-containing air in the tank is replaced by sterile air to ensure the fermentation quality of the material;
step four: checking the sealing performance of each part of the fermentation tank main body 1, and standing and fermenting for three days at normal temperature;
step five: and opening a valve at the discharge opening 12 to discharge the fermented materials, and cleaning the tank body to quickly enter the next fermentation work.
Further, in the fourth step, if the temperature is too high in summer, the tank body can be cooled by injecting cooling water into the water-proof heating jacket 2, the heat exchange area is the diameter of the fermentation tank, 3.14, of the height of the water-proof heating jacket 2, a water inlet and a water outlet of the cooling water need to be communicated with a water circulation water cooler to maintain the refrigeration temperature, the temperature in the tank is controlled at 30 ℃, and the tank is kept still for three days to finish the fermentation work; and in winter, clear water is required to be injected into the waterproof heating jacket 2, the water is heated by the water bath heating coil 18 in the waterproof heating jacket 2 to pass through the early heating stage, after the thalli generate heat in the growth stage, cooling water is introduced into the jacket, and the temperature is continuously reduced by virtue of an external circulating water cooler to be in a constant temperature state, so that the temperature is controlled at 30 ℃, and the time required by fermentation in winter is effectively shortened.
Further, in the fifth step, when the fermentation tank is cleaned, the water inlet connector 22 at the upper end of the sealing cover 6 can be communicated with a water pump, the water pump is used for conveying water into the annular spraying pipe 23 at the lower end of the sealing cover 6, and the nozzle 24 at the lower end of the annular spraying pipe 23 is used for cleaning the interior of the tank body, so that the cleaning operation is convenient and fast.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. The utility model provides a fermentation equipment of plant lactobacillus, includes fermentation cylinder main part (1), its characterized in that: the fermentation tank is characterized in that supporting legs (11) are installed below the fermentation tank main body (1), three supporting legs (11) are arranged, the three supporting legs (11) are all in welded connection with the fermentation tank main body (1), a water-resisting heating jacket (2) is fixedly installed outside the fermentation tank main body (1), a water inlet valve (3) is arranged at the upper end of one side of the water-resisting heating jacket (2), a water outlet valve (4) is arranged at the lower end of one side of the water-resisting heating jacket (2), a water bath heating coil (18) is arranged inside the water-resisting heating jacket (2), the water bath heating coil (18) is fixedly connected with the inner wall of the water-resisting heating jacket (2), wiring terminals (5) are fixedly installed at two ends of the water bath heating coil (18), the wiring terminals (5) penetrate through and extend to the water-resisting heating jacket (2), and the connection positions of the wiring terminals (5) and the water-resisting heating jacket (2) are, the upper end of the fermentation tank main body (1) is provided with a sealing cover (6), an internal thread mechanism (28) is arranged on the inner wall of the lower end of the sealing cover (6), the internal thread mechanism (28) and the sealing cover (6) are of an integrated structure, an external thread mechanism (29) is arranged on the outer wall of the upper end of the fermentation tank main body (1), the external thread mechanism (29) and the fermentation tank main body (1) are of an integrated structure, an annular spraying pipe (23) is arranged inside the sealing cover (6), the outer wall of the annular spraying pipe (23) is welded with the sealing cover (6), a water inlet connector (22) is fixedly connected onto the annular spraying pipe (23), one end of the water inlet connector (22) penetrates through and extends to the outside of the sealing cover (6), one end of the water inlet connector (22) is connected with a sealing head through threads, and a nozzle (24) is fixedly arranged at the lower end of, and the nozzle (24) is provided with twelve, the upper end of the sealing cover (6) is provided with a servo motor (15), the lower surface of the servo motor (15) is fixedly provided with a motor support (16), the servo motor (15) is fixedly connected with the sealing cover (6) through the motor support (16), the output end of the servo motor (15) is fixedly provided with a stirring shaft (19), one end of the stirring shaft (19) penetrates through the sealing cover (6) and extends into the fermentation tank main body (1), the joint of the stirring shaft (19) and the sealing cover (6) is fixedly provided with a mechanical sealing element (20), one end of the stirring shaft (19) is fixedly provided with a weighting block (27), one side of the servo motor (15) is provided with a feed inlet (13), the feed inlet (13) is fixedly connected with the sealing cover (6), the other side of the servo motor (15) is provided with a thermometer (17), and thermometer (17) and closing cap (6) threaded connection, the lower extreme fixed mounting of thermometer (17) has probe (21), and the one end of probe (21) runs through closing cap (6) and extends to the inside of fermentation cylinder main part (1), one side fixed mounting of fermentation cylinder main part (1) has admission valve (9), and the inner tube of admission valve (9) extends to the bottom of fermentation cylinder main part (1), the opposite side fixed mounting of fermentation cylinder main part (1) has discharge valve (10).
2. A fermentation apparatus of lactobacillus plantarum according to claim 1, characterized in that: the sealing cover (6) is in threaded connection with an external thread mechanism (29) at the upper end of the fermentation tank main body (1) through an internal thread mechanism (28) at the lower end.
3. A fermentation apparatus of lactobacillus plantarum according to claim 1, characterized in that: the joint of the sealing cover (6) and the fermentation tank main body (1) is provided with a rubber sealing ring (7), and the rubber sealing ring (7) is in interference fit with the fermentation tank main body (1).
4. A fermentation apparatus of lactobacillus plantarum according to claim 1, characterized in that: the stirring shaft is characterized in that spiral blades (25) are arranged on the outer wall of the stirring shaft (19), the spiral blades (25) are in threaded connection with the stirring shaft (19) through screws (26), and five screws (26) are arranged.
5. A fermentation apparatus of lactobacillus plantarum according to claim 1, characterized in that: the outer wall both sides of closing cap (6) all are provided with rotatory hangers (8), and rotatory hangers (8) and closing cap (6) welded connection.
6. A fermentation apparatus of lactobacillus plantarum according to claim 1, characterized in that: the inside of feed inlet (13) and discharge opening (12) all installs valve (14), and valve (14) and feed inlet (13) and discharge opening (12) sealing connection.
7. A working method of a fermentation device of lactobacillus plantarum is characterized by comprising the following steps:
the method comprises the following steps: preparing a lactobacillus plantarum starter, namely inoculating lactobacillus plantarum strains to an MRS agar plate, standing for two days at 35 ℃ to activate the strains, inoculating the activated lactobacillus plantarum into a liquid culture medium, standing for one day at 35 ℃ to obtain a seed solution, inoculating the seed solution into a propagation culture medium, and continuously standing for one day at 35 ℃ to obtain the lactobacillus plantarum starter;
step two: pouring materials to be fermented into the fermentation tank main body (1) from the feeding hole (13), and mixing the materials with the lactobacillus plantarum ferment agent 200: 1, adding a proper amount of lactobacillus plantarum ferment into the tank, starting a servo motor (15) to drive a stirring shaft (19) and a helical blade (25) on an output end to rotate, and fully mixing the material and the lactobacillus plantarum ferment;
step three: an air inlet valve (9) at one side of the fermentation tank main body (1) is communicated with an air compressor, one end of the air compressor is connected with a sterile air tank, and the air compressor is also installed at an exhaust valve (10) at the other side of the fermentation tank main body (1), so that the bacteria-containing air in the tank is replaced by sterile air to ensure the fermentation quality of the materials;
step four: after the tightness of each part of the fermentation tank main body (1) is checked, the fermentation tank main body is kept stand at normal temperature for 2 to three days;
step five: and opening a valve at the discharge opening (12), discharging the fermented materials, and cleaning the tank body to quickly enter the next fermentation work.
8. The working method of lactobacillus plantarum fermentation equipment according to claim 7, characterized in that: in the fourth step, if the temperature is too high in summer, the tank body can be cooled by injecting cooling water into the water-proof heating jacket (2), the heat exchange area is the diameter of the fermentation tank x 3.14 x the height of the water-proof heating jacket (2), a water inlet and a water outlet of the cooling water need to be communicated with a water circulation water cooler to maintain the refrigeration temperature, the temperature in the tank is controlled to be 30-35 ℃, and the tank is kept still for two to three days to finish the fermentation work; and when in winter, clear water is required to be injected into the waterproof heating jacket (2), water is heated through the water bath heating coil (18) in the waterproof heating jacket (2) to pass through the early heating stage, cooling water is introduced into the jacket after heat is generated in the thallus growth stage, the temperature is kept constant by means of continuous cooling of an external circulating water cooler, and the temperature is controlled to be 30-35 ℃, so that the time required by fermentation in winter is effectively shortened.
9. The working method of lactobacillus plantarum fermentation equipment according to claim 7, characterized in that: in the fifth step, when the fermentation tank is cleaned, the water inlet connector (22) at the upper end of the sealing cover (6) can be communicated with the water pump, the water pump is used for conveying water into the annular spraying pipe (23) at the lower end of the sealing cover (6), and the nozzle (24) at the lower end of the annular spraying pipe (23) is used for cleaning the inside of the tank body, so that the fermentation tank is convenient and fast.
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| CN113142264A (en) * | 2021-04-08 | 2021-07-23 | 柳志勇 | Water bath thermostatic type flour fermenting installation |
| CN115414997A (en) * | 2022-08-15 | 2022-12-02 | 吉林省佳园再生科技有限公司 | Animal carcass innocent treatment device |
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