CN115444825B - Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder - Google Patents
Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder Download PDFInfo
- Publication number
- CN115444825B CN115444825B CN202210636060.7A CN202210636060A CN115444825B CN 115444825 B CN115444825 B CN 115444825B CN 202210636060 A CN202210636060 A CN 202210636060A CN 115444825 B CN115444825 B CN 115444825B
- Authority
- CN
- China
- Prior art keywords
- licorice
- freeze
- radix paeoniae
- dried powder
- paeoniae alba
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 title claims abstract description 70
- 239000000843 powder Substances 0.000 title claims abstract description 39
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 235000014347 soups Nutrition 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 title claims description 47
- 244000303040 Glycyrrhiza glabra Species 0.000 title claims description 15
- 235000011477 liquorice Nutrition 0.000 title claims description 13
- 241000202807 Glycyrrhiza Species 0.000 claims abstract description 60
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims abstract description 55
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 claims abstract description 55
- 229940010454 licorice Drugs 0.000 claims abstract description 55
- 239000003814 drug Substances 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 244000236658 Paeonia lactiflora Species 0.000 claims abstract description 18
- 235000008598 Paeonia lactiflora Nutrition 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 239000008176 lyophilized powder Substances 0.000 claims abstract description 7
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 42
- 241000736199 Paeonia Species 0.000 claims description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 241001106477 Paeoniaceae Species 0.000 claims description 17
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 229960004949 glycyrrhizic acid Drugs 0.000 description 39
- 235000019410 glycyrrhizin Nutrition 0.000 description 36
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 35
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 35
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 description 28
- JBQATDIMBVLPRB-UHFFFAOYSA-N isoliquiritigenin Natural products OC1=CC(O)=CC=C1C1OC2=CC(O)=CC=C2C(=O)C1 JBQATDIMBVLPRB-UHFFFAOYSA-N 0.000 description 28
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 26
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 26
- DXDRHHKMWQZJHT-FPYGCLRLSA-N isoliquiritigenin Chemical compound C1=CC(O)=CC=C1\C=C\C(=O)C1=CC=C(O)C=C1O DXDRHHKMWQZJHT-FPYGCLRLSA-N 0.000 description 25
- 235000008718 isoliquiritigenin Nutrition 0.000 description 25
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 description 22
- HKQYGTCOTHHOMP-UHFFFAOYSA-N formononetin Chemical compound C1=CC(OC)=CC=C1C1=COC2=CC(O)=CC=C2C1=O HKQYGTCOTHHOMP-UHFFFAOYSA-N 0.000 description 22
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 description 22
- 239000004378 Glycyrrhizin Substances 0.000 description 21
- 239000001685 glycyrrhizic acid Substances 0.000 description 17
- 239000000126 substance Substances 0.000 description 16
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 description 14
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 description 14
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 description 14
- 239000005711 Benzoic acid Substances 0.000 description 13
- 235000010233 benzoic acid Nutrition 0.000 description 13
- 229940074391 gallic acid Drugs 0.000 description 13
- 235000004515 gallic acid Nutrition 0.000 description 13
- FTVWIRXFELQLPI-ZDUSSCGKSA-N (S)-naringenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC(O)=C2C(=O)C1 FTVWIRXFELQLPI-ZDUSSCGKSA-N 0.000 description 12
- LATYEZNGPQKAIK-UHFFFAOYSA-N 6'-O-benzoylpaeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(COC(=O)C=6C=CC=CC=6)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 LATYEZNGPQKAIK-UHFFFAOYSA-N 0.000 description 12
- LATYEZNGPQKAIK-HRCYFWENSA-N Benzoylpaeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](COC(=O)C=2C=CC=CC=2)O1)O)C)OC(=O)C1=CC=CC=C1 LATYEZNGPQKAIK-HRCYFWENSA-N 0.000 description 12
- VIWQCBZFJFSCLC-UHFFFAOYSA-N alpha-benzoyloxypaeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(COC(=O)C=6C=CC=CC=6)O5)O)CC3(O)OC1C24COC(=O)C1=CC=C(O)C=C1 VIWQCBZFJFSCLC-UHFFFAOYSA-N 0.000 description 12
- WGEYAGZBLYNDFV-UHFFFAOYSA-N naringenin Natural products C1(=O)C2=C(O)C=C(O)C=C2OC(C1)C1=CC=C(CC1)O WGEYAGZBLYNDFV-UHFFFAOYSA-N 0.000 description 12
- 229940117954 naringenin Drugs 0.000 description 12
- 235000007625 naringenin Nutrition 0.000 description 12
- RIKPNWPEMPODJD-UHFFFAOYSA-N formononetin Natural products C1=CC(OC)=CC=C1C1=COC2=CC=CC=C2C1=O RIKPNWPEMPODJD-UHFFFAOYSA-N 0.000 description 11
- QQUHMASGPODSIW-UHFFFAOYSA-N Albiflorin Natural products C=1C=CC=CC=1C(=O)OCC12C(=O)OC3(C)CC(O)C1CC32OC1OC(CO)C(O)C(O)C1O QQUHMASGPODSIW-UHFFFAOYSA-N 0.000 description 10
- QQUHMASGPODSIW-ICECTASOSA-N albiflorin Chemical compound O([C@@]12C[C@H]3[C@H](O)C[C@@]1(OC(=O)[C@]32COC(=O)C=1C=CC=CC=1)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O QQUHMASGPODSIW-ICECTASOSA-N 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 229940079593 drug Drugs 0.000 description 7
- 239000013558 reference substance Substances 0.000 description 7
- RETHOWGCGNZYSL-UHFFFAOYSA-N 6-[(11-carboxy-4,4,6a,6b,8a,11,14b-heptamethyl-14-oxo-2,3,4a,5,6,7,8,9,10,12,12a,14a-dodecahydro-1h-picen-3-yl)oxy]-5-[3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-3,4-dihydroxyoxane-2-carboxylic acid Chemical compound CC1(C)C2CCC3(C)C4(C)CCC5(C)CCC(C)(C(O)=O)CC5C4=CC(=O)C3C2(C)CCC1OC1OC(C(O)=O)C(O)C(O)C1OC1OCC(O)(CO)C1O RETHOWGCGNZYSL-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- KZNIFHPLKGYRTM-UHFFFAOYSA-N apigenin Chemical compound C1=CC(O)=CC=C1C1=CC(=O)C2=C(O)C=C(O)C=C2O1 KZNIFHPLKGYRTM-UHFFFAOYSA-N 0.000 description 6
- 229940117893 apigenin Drugs 0.000 description 6
- 235000008714 apigenin Nutrition 0.000 description 6
- XADJWCRESPGUTB-UHFFFAOYSA-N apigenin Natural products C1=CC(O)=CC=C1C1=CC(=O)C2=CC(O)=C(O)C=C2O1 XADJWCRESPGUTB-UHFFFAOYSA-N 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- LTINPJMVDKPJJI-UHFFFAOYSA-N iodinated glycerol Chemical compound CC(I)C1OCC(CO)O1 LTINPJMVDKPJJI-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- QREQQDSOBGSTFM-XXBRTPTLSA-N paeoniflorin b Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@@H](COC(=O)C=2C=CC=CC=2)O1)O)C)OC(=O)C1=CC=CC=C1 QREQQDSOBGSTFM-XXBRTPTLSA-N 0.000 description 5
- 239000013582 standard series solution Substances 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- GSZUGBAEBARHAW-UHFFFAOYSA-N sophoraflavone B Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C=2OC3=CC(O)=CC=C3C(=O)C=2)C=C1 GSZUGBAEBARHAW-UHFFFAOYSA-N 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- FURUXTVZLHCCNA-UHFFFAOYSA-N Liquiritigenin Natural products C1=CC(O)=CC=C1C1OC2=CC(O)=CC=C2C(=O)C1 FURUXTVZLHCCNA-UHFFFAOYSA-N 0.000 description 3
- 208000037386 Typhoid Diseases 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- FURUXTVZLHCCNA-AWEZNQCLSA-N liquiritigenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC=C2C(=O)C1 FURUXTVZLHCCNA-AWEZNQCLSA-N 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 201000008297 typhoid fever Diseases 0.000 description 3
- LPLVUJXQOOQHMX-MOGLOQIBSA-N (2s,3s,4s,5r,6r)-6-[(2r,3r,4s,5s,6s)-2-[[(3s,4ar,6ar,6bs,8as,11s,12ar,14ar,14bs)-11-carboxy-4,4,6a,6b,8a,11,14b-heptamethyl-14-oxo-2,3,4a,5,6,7,8,9,10,12,12a,14a-dodecahydro-1h-picen-3-yl]oxy]-6-carboxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-c Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-MOGLOQIBSA-N 0.000 description 2
- 235000017443 Hedysarum boreale Nutrition 0.000 description 2
- 235000007858 Hedysarum occidentale Nutrition 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 210000002435 tendon Anatomy 0.000 description 2
- KSDSYIXRWHRPMN-UHFFFAOYSA-N 4'-O-beta-D-Galactopyranoside-6''-p-Coumaroylprunin-4',5,7-Trihydroxyflavanone Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC(O)=C3C(=O)C2)C=C1 KSDSYIXRWHRPMN-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 description 1
- 206010063659 Aversion Diseases 0.000 description 1
- AVGPOAXYRRIZMM-UHFFFAOYSA-N D-Apiose Natural products OCC(O)(CO)C(O)C=O AVGPOAXYRRIZMM-UHFFFAOYSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-LJJLCWGRSA-N D-apiofuranose Chemical compound OC[C@@]1(O)COC(O)[C@@H]1O ASNHGEVAWNWCRQ-LJJLCWGRSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-UHFFFAOYSA-N D-apiofuranose Natural products OCC1(O)COC(O)C1O ASNHGEVAWNWCRQ-UHFFFAOYSA-N 0.000 description 1
- DEMKZLAVQYISIA-ONJCETCRSA-N Liquiritin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)c1ccc([C@@H]2Oc3c(C(=O)C2)ccc(O)c3)cc1 DEMKZLAVQYISIA-ONJCETCRSA-N 0.000 description 1
- DEMKZLAVQYISIA-UHFFFAOYSA-N Liquirtin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-UHFFFAOYSA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 206010062575 Muscle contracture Diseases 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- NTDLXWMIWOECHG-YRCFQSNFSA-N apiin Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O[C@H]1[C@@H]([C@@](O)(CO)CO1)O)O)CO)C(C=1)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C=C1 NTDLXWMIWOECHG-YRCFQSNFSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 208000006111 contracture Diseases 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- DEMKZLAVQYISIA-ZRWXNEIDSA-N liquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C([C@H]2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-ZRWXNEIDSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 description 1
- 229930019673 naringin Natural products 0.000 description 1
- 229940052490 naringin Drugs 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/14—Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Neurology (AREA)
- Vascular Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of radix paeoniae alba and licorice decoction freeze-dried powder, belonging to the technical field of traditional Chinese medicine preparations, wherein the preparation method is to add radix paeoniae alba into an ethanol water solution with concentration of 80-90 wt%, adjust the pH value, soak, and then cover and decoct the radix paeoniae alba to obtain radix paeoniae alba liquid medicine; adding Glycyrrhrizae radix into water, adjusting pH, soaking, decocting, and filtering to obtain Glycyrrhrizae radix liquid medicine; mixing radix Paeoniae alba liquid medicine and Glycyrrhrizae radix liquid medicine, concentrating, and lyophilizing to obtain the radix Paeoniae and Glycyrrhrizae radix soup lyophilized powder. The 17 effective components in the Paeonia lactiflora and licorice decoction prepared by the invention are improved compared with the decoction method in the classical prescription, and the curative effect of the Paeonia lactiflora and licorice decoction can be effectively improved.
Description
Technical Field
The invention relates to preparation of traditional Chinese medicine freeze-dried powder, in particular to a preparation method of peony and licorice soup freeze-dried powder.
Background
The Paeonia lactiflora and licorice decoction is the 6 th prescription in the category of classical names (first batch). The original text comes from the treatise on typhoid fever (Han. Zhang Zhongjing), and is described as "the typhoid fever pulse is superficial, spontaneous sweating, frequent urination, vexation, slight aversion to cold, foot contracture. … … for foot warming, it is combined with Paeonia radix Glycyrrhizae decoction to stretch the foot. The original text records the prescription composition of four or two of radix paeoniae alba and radix glycyrrhizae respectively, and the prescription composition is roasted. The two medicines are boiled with three liters of water, one liter is boiled, five times are combined, dregs are removed, and the medicines are taken after being separated into temperature. "according to the literature and examination data, the modern application dosage and processed product are determined by research: white peony root 12g, water-roasted licorice 12g. Has effects of softening liver, relaxing muscles and tendons, relieving spasm and pain. Can be used for treating yin blood, body fluid deficiency, and malnutrition of tendons and vessels, and can also be used for preventing heart disease and treating varicosis.
The original text records that the decoction method is that the two medicines are decocted by three liters of water, one liter of five medicines are decocted, and the decoction is calculated according to the fact that the modern volume is 200mL and the modern volume is 20mL by one liter of Chinese instead of one liter, the Chinese herbaceous peony and licorice decoction is converted into the two medicines in the modern preparation method, 600mL of water is added, and the decoction is decocted until the liquid medicine is 300mL. However, the method is time-consuming, inconvenient to take, and unfavorable for quality control and use.
Disclosure of Invention
Aiming at the problems, the invention provides a preparation method of freeze-dried powder of paeonia lactiflora and licorice decoction.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the preparation method of the freeze-dried powder of the peony and licorice soup is characterized by comprising the following steps of:
adding white paeony root into an ethanol water solution with the concentration of 80-90 wt%, regulating the pH value to 6.0-6.5, soaking, covering and decocting, and filtering to obtain white paeony root liquid medicine;
adding licorice into water, regulating pH to 7.2-7.5, soaking, decocting, filtering to obtain licorice liquid;
mixing radix Paeoniae alba liquid medicine and Glycyrrhrizae radix liquid medicine, concentrating, and lyophilizing to obtain the radix Paeoniae and Glycyrrhrizae radix soup lyophilized powder.
Further, the dosage of the ethanol aqueous solution is 7 to 8 times of the weight of the white paeony root; the water consumption is 8-10 times of the weight of the liquorice.
Further, the white paeony root is decocted for 1.5 to 2 hours by slow fire; the liquorice is decocted for 2-3 hours by slow fire.
Further, the soaking time of the white paeony root and the liquorice is 0.5-1 h.
Further, the concentration is carried out under the conditions of 60-80 ℃ and-0.08 to-0.1 MPa.
Further, after concentration and before freeze drying, the total weight ratio of the concentrated solution to the white paeony root and the liquorice is 1.8-2.2: 1.
further, the freeze drying is to pre-freeze at-30 to-45 ℃ for at least 60min, and then vacuum drying at-40 to-70 ℃ for at least 12h.
Further, the vacuum degree of vacuum drying is less than 100Pa.
Further, the weight ratio of the white paeony root to the liquorice is 1:1.
the preparation method of the freeze-dried powder of the peony and licorice soup has the beneficial effects that:
according to the invention, the 17 most main effective components in the peony and licorice soup are detected, and the different characteristics of the radix paeoniae alba and the licorice are utilized, and different solvents are adopted for extraction respectively, so that the content of the 17 effective components in the finally prepared freeze-dried powder of the peony and licorice soup can be effectively increased;
according to the invention, effective components such as gallic acid, isoliquiritigenin, paeonol, albiflorin, paeoniflorin, albiflorin B, benzoic acid, naringenin and benzoylpaeoniflorin and the like extracted from radix paeoniae alba are examined, and apigenin, glycyrrhizin, formononetin, neoglycyrrhizin, 3' -deoxidized Su Mutong A, isoliquiritigenin, glycyrrhizin and glycyrrhizic acid extracted from radix paeoniae alba are extracted under different extraction conditions by selecting extraction solvents which are more suitable for radix paeoniae alba and radix glycyrrhizae, so that the extraction quantity of the effective components is effectively increased;
17 effective components in the radix paeoniae alba and licorice decoction prepared by the invention are improved compared with the decoction method in the classical prescription, so that the curative effect of the radix paeoniae alba and licorice decoction can be effectively improved;
according to the invention, through selecting proper concentration and freeze-drying processes, the freeze-dried powder is prepared under the condition of ensuring that the content of the effective components is not reduced as much as possible, so that the convenience of storage and administration is improved;
the invention has simple production process and low solvent consumption, can save the time of concentration and freeze-drying, and reduces the production cost.
Drawings
FIG. 1 is a high performance liquid chromatogram of lyophilized powder of radix Paeoniae and Glycyrrhrizae radix decoction in embodiment 1 of the invention;
fig. 2 is a high performance liquid chromatogram of the freeze-dried powder of the peony and licorice decoction in comparative example 1 of the present invention.
Detailed Description
The following description of the technical solution in the embodiments of the present invention is clear and complete. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.
Example 1 preparation method of Paeonia lactiflora and Glycyrrhiza decoction lyophilized powder
The embodiment is a preparation method of radix paeoniae alba and liquorice soup freeze-dried powder, and the specific synthetic process comprises the following steps:
1) Taking 12g of white paeony root, adding 8 times of 85wt% ethanol water solution (96 g), dripping a small amount of 10wt% hydrochloric acid to adjust the pH value to 6.5, soaking for 1h, adding a cover, decocting with slow fire for 1.5h, and filtering by a 200-mesh nylon sieve at normal pressure to obtain white paeony root liquid medicine;
2) Adding water (108 g) which is 9 times of licorice into 12g of licorice, dropwise adding a small amount of 10wt% sodium hydroxide aqueous solution to adjust the pH value to 7.4, soaking for 1h, directly decocting with slow fire for 2.5h without covering, and filtering by a 200-mesh nylon sieve at normal pressure to obtain licorice liquid medicine;
3) Mixing radix Paeoniae alba liquid medicine and Glycyrrhrizae radix liquid medicine, concentrating under reduced pressure at 70deg.C and-0.1 MPa, stopping concentrating until the obtained concentrated solution is 48g, transferring into freeze dryer, pre-freezing at-35deg.C for 60min, cooling to-50deg.C, vacuum drying at-50deg.C under vacuum degree less than 100Pa for 12 hr, and pulverizing to obtain 7.47g radix Paeoniae Glycyrrhrizae radix decoction lyophilized powder marked as M1. The obtained radix paeoniae alba and licorice decoction freeze-dried powder is dry paste powder of radix paeoniae alba and licorice decoction, and the dry paste rate is 32.25%.
The content of 17 effective components of the freeze-dried powder M1 of the peony and licorice decoction is measured by high performance liquid chromatography, the obtained chromatogram is shown in figure 1, and the specific measuring method is as follows:
b1 Preparation of test solution and reference solution
b11 Preparation of test sample solution
0.1g of the freeze-dried powder M1 (namely M) of the peony and licorice decoction is precisely weighed out Radix paeoniae alba =0.1 g), put into a 10mL measuring flask, add 0.5wt% sodium hydroxide aqueous solution to dissolve and dilute to scale, ultrasound for 100min under 40kHz and 300W, cool to obtain dissolution liquid;
precisely transferring 1mL of the dissolution liquid, placing the dissolution liquid in a 5mL measuring flask, diluting with ethanol, fixing the volume to a scale, and shaking uniformly to obtain a sample solution; at this time, the freeze-drying of 0.1g of Paeonia lactiflora and Glycyrrhiza decoction corresponds to the volume of the prepared test solution of V Test on test ＝50mL。
b12 Preparation of control stock solution
The preparation method comprises the steps of respectively taking appropriate amounts of gallic acid, isoliquiritigenin, paeonol, albiflorin, paeoniflorin B, benzoic acid, naringenin, benzoylpaeoniflorin, apioglycyrrhizin, glycyrrhizin, formononetin, neoglycyrrhizin, 3' -deoxy Su Mutong A, isoliquiritigenin, glycyrrhizin and glycyrrhizic acid reference substances, precisely weighing, dissolving and diluting with 70wt% ethanol aqueous solution to obtain a solution with concentration of 135.274 mug/mL (gallic acid), 40.125 mug/mL (concentration of isoliquiritigenin), 0.415 mug/mL (concentration of paeonol), 86.249 mug/mL (concentration of paeoniflorin), 318.615 mug/mL (concentration of paeoniflorin), 431.743 mug/mL (concentration of apioglycyrrhizin), 95.893 mug/mL (concentration of glycyrrhizin), 6.124 mug/mL (concentration of paeoniflorin B), 4.320 mug/mL (concentration of benzoic acid), 10.410 mug/mL (concentration of naringin), 7.920 muginein/mL (concentration of 3832 muginein), stock solution with concentration of 3 muginein (concentration of 3 muginein/mL (concentration of 3 muginein), and 37 muginein (concentration of 3.3737 muginein) of 3 muginein (concentration of 3 muginein).
b13 Standard series solutions of different concentrations of reference substances
Precisely measuring 1, 2, 4, 5, 8 and 10mL of reference substance stock solutions respectively, placing the reference substance stock solutions in 50, 20, 10 and 10mL measuring flasks, and fixing the volumes by using 70wt% ethanol water solution to serve as reference substance standard series solutions with different concentrations, wherein the concentrations of different chemical components are shown in Table 1.
b2 High performance liquid chromatography detection
b21 Taking reference standard series solutions with different concentrations, and performing high performance liquid chromatography detection under the above chromatographic conditions to obtain peak areas of corresponding chemical components measured according to the concentrations, wherein the specific peak areas are shown in Table 1.
Table 1A list of test results for Standard series solutions of reference substances at different concentrations
Wherein, the high performance liquid chromatography conditions of the standard solution of the detection reference substance are as follows:
chromatographic column: YMC-Triart C18 (5 μm, 4.6X1250 mm);
mobile phase: acetonitrile (a) -0.1wt% formic acid in water (B);
elution procedure: 0-5 min,5% -13% mobile phase A,95% -87% mobile phase B;
5-15 min,13% -23% mobile phase A,87% -77% mobile phase B;
15-40 min,23% -50% mobile phase A,77% -50% mobile phase B;
40-55 min,50% -100% mobile phase A,50% -0% mobile phase B;
55-55.01 min,100% -5% mobile phase A,0% -95% mobile phase B;
55.01-65 min,5% mobile phase A,95% mobile phase B.
Flow rate: 0.60mL/min;
column temperature: 35 ℃;
detection wavelength: 230nm;
sample injection amount: 5. Mu.L.
And drawing a standard curve corresponding to the different chemical components according to the concentrations of the different chemical components and the peak areas measured according to the concentrations of the corresponding chemical components, wherein the concentrations of the different chemical components are taken as an abscissa (namely x, the unit is mu g/mL), and the peak areas measured according to the concentrations of the corresponding chemical components are taken as an ordinate (namely y).
The standard curves drawn with the reference standard series solutions of different concentrations are as follows:
the standard curve corresponding to gallic acid is y=7893.9x+10.438, r 2 ＝1.000；
The standard curve corresponding to isoliquiritigenin is y=9517x+8090.5, R 2 ＝0.9999；
Paeonol has standard curve of y= 874711x-597.18, R 2 ＝1.000；
The standard curve corresponding to the paeoniflorin is y=4789 x-1561.8, R 2 ＝0.9995；
The standard curve corresponding to paeoniflorin is y=12301x+21868, R 2 ＝0.9996；
The standard curve corresponding to apigenin is y= 8142.2x-3638.1, R 2 ＝0.9993；
The standard curve corresponding to glycyrrhizin is y=19786x+11017, R 2 ＝0.9996；
The standard curve corresponding to the albino B is y=47442x+629.14, r 2 ＝0.9996；
Benzoic acid corresponds to a standard curve y= 106956x-899.14, r 2 ＝1.000；
Naringenin has a standard curve of y= 40543x-1700.9, R 2 ＝0.9996；
The standard curve corresponding to formononetin is y= 40417x-1051.9, R 2 ＝0.9994；
The standard curve corresponding to the new glycyrrhizin is y= 61161x-1294, R 2 ＝0.9998；
The standard curve corresponding to 3' -deoxy Su Mutong a is y=49390x+298.4, r 2 ＝0.9999；
Isoliquiritigenin has a standard curve of y= 28818x-272.07, R 2 ＝0.9998；
The standard curve corresponding to the glycyrrhizin isy＝16997x+371.77，R 2 ＝0.9998；
The standard curve corresponding to the benzoylpaeoniflorin is y= 1684.4x-587.75, R 2 ＝0.9999；
The standard curve corresponding to glycyrrhizic acid is y=5868.1x+14514, R 2 ＝0.9995。
b23 Taking the sample solution, performing high performance liquid chromatography detection under the above chromatographic conditions to obtain peak areas corresponding to different chemical components contained in the sample solution, substituting the peak areas of the corresponding chemical components into corresponding standard curves to obtain the concentration (C) of the corresponding chemical components in the sample solution Test-corresponding chemical composition )。
And then the concentration (C) of the corresponding chemical component in the sample solution Test-corresponding chemical composition ) Multiplied by the total volume of the prepared test solution (V Test on test =50 mL) divided by the amount of the freeze-dried powder of the peony and licorice decoction used for preparing the test solution (m Radix paeoniae alba =0.1 g), i.e. the content of the corresponding chemical component in the peony and licorice soup, that is to say the content of the corresponding chemical component in the peony and licorice soup= (C) Para-corresponding chemical composition ×V Test on test )/m Radix paeoniae alba 。
In the case of glycyrrhizic acid, the peak area (A) Para-glycyrrhizic acid = 436727) substituting the standard curve corresponding to glycyrrhizic acid, and calculating to obtain the concentration (C) Glycyrrhizinic acid ＝71.95μg/mL)；
Content of glycyrrhizic acid in Paeonia lactiflora and Glycyrrhiza decoction= (C) Para-glycyrrhizic acid ×V I-glycyrrhizic acid )/m Radix paeoniae alba ＝65.134μg/mL×50mL/0.1g＝29606.5μg/g＝35.98mg/g。
In the embodiment, the paeonia lactiflora decoction M1 contains 2.074mg/g of gallic acid, 3.321mg/g of isoliquiritigenin, 0.0058mg/g of paeonol, 1.369mg/g of paeoniflorin, 52.71mg/g of paeoniflorin, 0.1023mg/g of paeoniflorin B, 0.0676mg/g of benzoic acid, 0.1529mg/g of naringenin, 5.524mg/g of benzoylpaeoniflorin, 28.64mg/g of apioglycyrrhizin, 26.19mg/g of glycyrrhizin, 0.1217mg/g of formononetin, 0.1034mg/g of neoglycyrrhizin, 0.1426mg/g of 3' -deoxysappan ketone A, 0.2312mg/g of isoliquiritigenin, 0.2628mg/g of glycyrrhizin and 47.98mg/g of glycyrrhizic acid.
Equivalently, the analysis of the components in the preparation of the peony and licorice decoction M1 in this example is specifically shown in the following table:
TABLE 2 list of results of active ingredients in Paeonia lactiflora and Glycyrrhiza decoction
| Active ingredient | Content of active ingredient (mg/g) | Total amount of each active ingredient | Extraction efficiency (%) |
| Gallic acid | 2.074 | 16.05276 | 0.134 |
| Isoglycyrrhizin | 3.321 | 25.70454 | 0.214 |
| Paeonol | 0.0058 | 0.044892 | 0.0004 |
| Albiflorin | 1.369 | 10.59606 | 0.0883 |
| Paeoniflorin | 52.71 | 407.9754 | 0.34 |
| Apiose glycyrrhizin | 28.64 | 221.6736 | 1.85 |
| Liquiritigenin | 26.19 | 202.7106 | 1.69 |
| Albiflorin B | 0.1023 | 0.791802 | 0.0066 |
| Benzoic acid | 0.0676 | 0.523224 | 0.0044 |
| Naringenin | 0.1529 | 1.183446 | 0.0099 |
| Formononetin-like element | 0.1217 | 0.941958 | 0.0078 |
| Novel glycyrrhizin | 0.1034 | 0.800316 | 0.0067 |
| 3' -deoxy Su Mutong A | 0.1426 | 1.103724 | 0.0092 |
| Isoliquiritigenin | 0.2312 | 1.789488 | 0.0149 |
| Liquiritigenin | 0.2628 | 2.034072 | 0.017 |
| Benzoyl paeoniflorin | 5.524 | 42.75576 | 0.356 |
| Glycyrrhizic acid | 47.98 | 371.3652 | 2.32 |
EXAMPLES 2-6 preparation method of Paeonia lactiflora and Glycyrrhiza decoction lyophilized powder
Examples 2 to 6 are respectively a preparation method of radix paeoniae alba and licorice decoction freeze-dried powder, and the steps are basically the same as those of example 1, except that the raw material dosage and the technological parameters are different, and the specific details are shown in table 3:
table 3 list of process parameters in examples 2 to 5
The other parts of examples 2 to 6 are the same as in example 1.
Determination of Experimental example 1 content
Comparative examples 1 to 6 are comparative experiments of the preparation process of the freeze-dried powder of the peony and licorice decoction in example 1, and the difference between the radix paeoniae alba and the licorice is that the same batch of medicinal materials is taken from example 1, and the difference is that:
in comparative example 1, four or two of the Chinese herbaceous peony and licorice root are processed according to the description of typhoid fever. The two above two medicines are boiled with three liters of water, one liter of five medicines is removed, the dregs are separated, then the mixed liquor of white paeony root and liquorice is prepared, and then the mixed liquor is concentrated and freeze-dried by the method in the example 1 to prepare 5.04 white paeony root and liquorice soup freeze-dried powder, wherein the dry paste rate is 21.00 percent, and the white paeony root and liquorice soup freeze-dried powder DM1 contains 1.614mg/g gallic acid, 0.8241mg/g isoliquiritigenin, 0.0045mg paeonol, 0.8632mg of paeoniflorin, 41.44mg/g paeoniflorin, 0.0678mg/g paeoniflorin, 0.0459mg/g benzoic acid, 0.1042mg/g naringenin, 3.512mg/g benzoylpaeoniflorin, 24.12mg/g apigenin, 19.69mg/g glycyrrhizin, 0.0798mg formononetin, 0.0657mg/g neoglycyrrhizin, 3' -deoxidized Su Mutong A0.0869 mg/g, 0.1534mg isoliquiritigenin and 0.1856.63 mg liquorice.
1.614mg/g of gallic acid, 0.8241mg/g of isoliquiritigenin, 0.0045mg/g of paeonol, 0.8632mg/g of paeoniflorin, 41.44mg/g of paeoniflorin, 0.0678mg/g of paeoniflorin B, 0.0459mg/g of benzoic acid, 0.1042mg/g of naringenin, 3.512mg/g of benzoylpaeoniflorin, 24.12mg/g of apigenin, 19.69mg/g of liquiritin, 0.0798mg/g of formononetin, 0.0657mg/g of neoglycyrrhizin, 0.0869mg/g of 3' -deoxy Su Mutong A, 0.1534mg/g of isoliquiritigenin, 0.1856mg/g of liquiritigenin and 29.61mg/g of glycyrrhizic acid.
In the step 1) in the comparative example 2, only 85wt% of ethanol aqueous solution is replaced by 100% ethanol, the obtained 6.86g of radix paeoniae alba and licorice soup freeze-dried powder is marked as DM2, the dry paste rate is 28.58%, and the radix paeoniae alba and licorice soup freeze-dried powder DM2 contains 2.121mg/g gallic acid, 3.314mg/g isoliquiritigenin, 0.0062mg/g paeonol, 0.657mg/g albiflorin, 34.24mg/g paeoniflorin, 0.8793mg/g paeoniflorin, 0.0423mg/g benzoic acid, 0.1293mg/g naringenin, 4.912mg/g benzoyl paeoniflorin, 28.38mg/g apigenin, 26.27mg/g glycyrrhizin, 0.1224mg/g formononetin, 0.1021mg/g neoglycyrrhizin, 3' -deoxidized Su Mutong A0.1409 mg/g isoliquiritigenin 0.2321mg/g, 0.2619mg/g glycyrrhizin and 47.93mg glycyrrhizic acid; it can be seen that the content of the effective components of the white peony root is obviously reduced.
In the step 1) in the comparative example 3, only 85wt% of ethanol aqueous solution is replaced by 60wt% of ethanol aqueous solution, the obtained 6.58g of radix paeoniae alba and licorice soup freeze-dried powder is marked as DM3, the dry paste rate is 27.38%, and the radix paeoniae alba and licorice soup freeze-dried powder DM3 contains 1.0123mg/g gallic acid, 3.327mg/g isoliquiritigenin, 0.0041mg/g paeonol, 0.8264mg of paeoniflorin, 38.71mg/g paeoniflorin, 0.9153mg/g paeoniflorin, 0.0375mg/g benzoic acid, 0.1497mg/g naringenin, 2.524mg/g benzoylpaeoniflorin, 28.72mg/g apigenin, 25.98mg/g glycyrrhizin, 0.1231mg/g formononetin, 0.1042mg/g neoglycyrrhizin, 0.1410mg/g 3' -deoxysappan, 0.2306mg isoliquiritigenin, 0.2617mg/g and 48.04mg glycyrrhizic acid; it can be seen that the content of part of the effective components in the white paeony root is obviously reduced.
In the step 1) of the comparative example 4, the pH value is not adjusted, the obtained 6.72g of the freeze-dried powder of the Paeonia lactiflora decoction is directly soaked in an ethanol aqueous solution, the dry paste rate is 28.00 percent, and the DM4 of the Paeonia lactiflora decoction contains 1.845mg/g gallic acid, 3.321mg/g isoliquiritigenin, 0.0047mg/g paeonol, 1.126mg/g paeoniflorin, 32.69mg/g paeoniflorin, 0.0824mg/g paeoniflorin B, 0.0563mg/g benzoic acid, 0.1392mg/g naringenin, 4.524mg/g benzoyl paeoniflorin, 28.69mg/g apioglycoside, 25.94mg/g glycyrrhizin, 0.1236mg/g formononetin, 0.1029mg/g neoglycyrrhizin, 3' -deoxy Su Mutong A0.1419 mg/g, 0.2324mg isoliquiritigenin 0.2626mg/g and 47.85mg glycyrrhizic acid; it can be seen that the content of part of the effective components in the white paeony root is obviously reduced.
In the step 1) of the comparative example 5, the obtained 7.09g of freeze-dried powder of the peony licorice soup, marked as DM5, has a dry paste rate of 29.54%, and the freeze-dried powder DM5 of the peony licorice soup contains 2.076mg/g gallic acid, 3.325mg/g isoliquiritigenin, 0.0007mg/g paeonol, 1.321mg/g albiflorin, 49.69mg/g paeoniflorin, 0.1034mg/g albiflorin B, 0.0667mg/g benzoic acid, 0.1534mg/g naringenin, 5.521mg/g benzoylpaeoniflorin, 28.72mg/g apioglycoside, 26.21mg/g glycyrrhizin, 0.1214mg/g formononetin, 0.1039mg/g neoglycyrrhizin, 3' -deoxy Su Mutong A0.1424 mg/g isoliquiritigenin, 0.2316mg/g isoliquiritigenin, 0.2625mg/g glycyrrhizin and 47.91mg/g glycyrrhizic acid; it can be seen that the content of paeonol and other effective components in the white paeony root is obviously reduced.
In the step 2) of the comparative example 6, the pH value is not regulated, water is directly added for soaking, the obtained 6.96g of freeze-dried powder of the peony licorice soup is marked as DM6, the dry paste rate is 29.00 percent, and the freeze-dried powder DM6 of the peony licorice soup contains 2.076mg/g gallic acid, 2.672mg/g isoliquiritigenin, 0.0057mg/g paeonol, 1.364mg/g albiflorin, 52.68mg/g paeoniflorin, 0.1026mg/g albiflorin B, 0.0678mg/g benzoic acid, 0.1524mg naringenin, 5.531mg/g benzoyl paeoniflorin, 22.92mg/g apioside, 20.71mg/g glycyrrhizin, 0.0954mg of formononetin, 0.0623mg/g neoglycyrrhizin, 0.0324mg/g 3' -deoxidized Su Mutong A, 0.1059mg isoliquiritigenin, 0.1379mg/g glycyrrhizin and 26.15mg/g glycyrrhizic acid; it can be seen that the content of the effective components in the licorice root is obviously reduced.
It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Claims (9)
1. The preparation method of the freeze-dried powder of the peony and licorice soup is characterized by comprising the following steps of:
adding white paeony root into an ethanol water solution with the concentration of 80-90 wt%, regulating the pH value to 6.0-6.5, soaking, covering and decocting, and filtering to obtain white paeony root liquid medicine;
adding licorice into water, adjusting the pH value to 7.2-7.5, soaking, decocting, and filtering to obtain licorice liquid medicine;
mixing radix Paeoniae alba liquid medicine and Glycyrrhrizae radix liquid medicine, concentrating, and lyophilizing to obtain the radix Paeoniae and Glycyrrhrizae radix soup lyophilized powder.
2. The method for preparing freeze-dried powder of paeonia lactiflora and licorice decoction according to claim 1, wherein the dosage of the ethanol aqueous solution is 7-8 times of the weight of the paeonia lactiflora; the water is 8-10 times of the licorice by weight.
3. The method for preparing the freeze-dried powder of the peony and licorice decoction according to claim 1 or 2, wherein the radix paeoniae alba is decocted with slow fire for 1.5-2 hours; the liquorice is decocted for 2-3 hours by slow fire.
4. The method for preparing the freeze-dried powder of the peony and licorice soup according to claim 1 or 2, wherein the soaking time of the radix paeoniae alba and the licorice is 0.5-1 h.
5. The preparation method of the freeze-dried powder of paeonia lactiflora and licorice decoction according to claim 1 or 2, wherein the concentration is carried out under reduced pressure at 60-80 ℃ and-0.08 to-0.1 MPa.
6. The method for preparing the freeze-dried powder of the peony and licorice soup according to claim 1 or 2, wherein the total weight ratio of the concentrated solution to the radix paeoniae alba and the licorice is 1.8-2.2 after concentration and before freeze-drying: 1.
7. the method for preparing the freeze-dried powder of the peony and licorice soup according to claim 1 or 2, wherein the freeze-drying is performed by pre-freezing at-30 to-45 ℃ for at least 60min and then vacuum drying at-40 to-70 ℃ for at least 12h.
8. The method for preparing freeze-dried powder of paeonia lactiflora and licorice decoction according to claim 7, wherein the vacuum degree of vacuum drying is less than 100Pa.
9. The method for preparing the freeze-dried powder of the peony and licorice soup according to claim 1 or 2, wherein the weight ratio of the radix paeoniae alba to the licorice is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210636060.7A CN115444825B (en) | 2022-06-07 | 2022-06-07 | Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210636060.7A CN115444825B (en) | 2022-06-07 | 2022-06-07 | Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115444825A CN115444825A (en) | 2022-12-09 |
| CN115444825B true CN115444825B (en) | 2024-01-26 |
Family
ID=84297001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210636060.7A Active CN115444825B (en) | 2022-06-07 | 2022-06-07 | Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115444825B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116159095B (en) * | 2023-02-03 | 2024-03-22 | 神威药业集团有限公司 | Preparation method of angelica hexa-yellow Shang Dong dry powder |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204951A (en) * | 2011-05-24 | 2011-10-05 | 药都制药集团股份有限公司 | Method for extracting active components from licorice |
| CN112773831A (en) * | 2020-12-21 | 2021-05-11 | 贵州景诚制药有限公司 | Preparation method and detection method of peony and liquorice pharmaceutical composition |
| CN113230316A (en) * | 2021-06-04 | 2021-08-10 | 郑州大学 | Application of peony and licorice decoction preparation in preventing and treating tumor diseases |
| CN113820422A (en) * | 2021-09-24 | 2021-12-21 | 宁波立华制药有限公司 | Method for detecting total glucosides of paeony by fingerprint spectrum |
| CN114376952A (en) * | 2021-12-31 | 2022-04-22 | 浙江大学 | Paeonia lactiflora flower extract and preparation method and application thereof |
-
2022
- 2022-06-07 CN CN202210636060.7A patent/CN115444825B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204951A (en) * | 2011-05-24 | 2011-10-05 | 药都制药集团股份有限公司 | Method for extracting active components from licorice |
| CN112773831A (en) * | 2020-12-21 | 2021-05-11 | 贵州景诚制药有限公司 | Preparation method and detection method of peony and liquorice pharmaceutical composition |
| CN113230316A (en) * | 2021-06-04 | 2021-08-10 | 郑州大学 | Application of peony and licorice decoction preparation in preventing and treating tumor diseases |
| CN113820422A (en) * | 2021-09-24 | 2021-12-21 | 宁波立华制药有限公司 | Method for detecting total glucosides of paeony by fingerprint spectrum |
| CN114376952A (en) * | 2021-12-31 | 2022-04-22 | 浙江大学 | Paeonia lactiflora flower extract and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 不同评价指标对芍药甘草汤提取工艺优化的影响;杜平等;中成药;第40卷(第10期);第2188-2193页 * |
| 芍药甘草汤HPLC指纹图谱的建立和7种成分含量测定;何利等;中药材;第42卷(第08期);第1837-1841页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115444825A (en) | 2022-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU658614B2 (en) | Pharmaceutical compositions for the treatment of skin disorders | |
| CN1364468A (en) | Wall breaking and extracting method for Chinese herbal medicine and formulation | |
| CN111840363B (en) | Natto-assisted hypoglycemic health-care composition and preparation method and application thereof | |
| CN115444825B (en) | Preparation method of radix paeoniae alba and liquorice soup freeze-dried powder | |
| CN107308234A (en) | A kind of preparation method of guizhi fuling pill | |
| CN102283913A (en) | Radix aconiti carmichaeli extract and application thereof | |
| CN112516219B (en) | Preparation process of traditional Chinese medicine granules for treating systemic lupus erythematosus | |
| CN103316074B (en) | Medicine composite of halenia corni extract, astragalus extract and liquorice extract as well as preparation and application of medicine composite | |
| CN101869675B (en) | Chinese medicinal composition granules for treating summer-heat damp cold and preparation method thereof | |
| CN100455300C (en) | Compound wild kiwi tablet and its preparing method | |
| CN104474143B (en) | A kind of Chinese medicine composition and preparation method thereof for treating hepatitis B | |
| CN114145374A (en) | Astragalus membranaceus and corn stigma composite tea and preparation method and application thereof | |
| CN111150750A (en) | Processing method and application of wine-processed cornus officinalis | |
| CN100488543C (en) | Local externally applied itch stopping garlic liniments | |
| CN106177416B (en) | A kind of traditional Chinese medicinal composition with effect of reducing blood sugar and preparation method thereof | |
| CN112618425A (en) | Preparation method of multi-effect repairing, moistening and hair nourishing essence | |
| AU2015203092B1 (en) | Pharmaceutical composition for treatment of menopausal syndrome and preparation method thereof | |
| CN108042682A (en) | A kind of Long Lu Zishuiqinggan ball and preparation method thereof | |
| CN114306415B (en) | Preparation method for improving content of index component in pulp powder of dogwood fruit | |
| CN103961602B (en) | The treatment Chinese medicine composition of chronic gastritis and tablet thereof and preparation method | |
| CN115919939B (en) | Traditional Chinese medicine composition for treating hyperplasia of mammary glands and preparation method and application thereof | |
| CN107952012A (en) | A kind of Chinese medicine composition for preventing global-worm illness and preparation method thereof | |
| CN1096195A (en) | The preparation method and the purposes of Chinese medicine paste freckle-removing mask | |
| CN104524044A (en) | Preparation method of Zhixinkang | |
| CN116370552A (en) | Lotion and extraction process of extract thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |