CN115443998B - High-specificity spodoptera frugiperda sex attractant and application thereof - Google Patents
High-specificity spodoptera frugiperda sex attractant and application thereof Download PDFInfo
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- CN115443998B CN115443998B CN202211267495.5A CN202211267495A CN115443998B CN 115443998 B CN115443998 B CN 115443998B CN 202211267495 A CN202211267495 A CN 202211267495A CN 115443998 B CN115443998 B CN 115443998B
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- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 61
- 239000000877 Sex Attractant Substances 0.000 title claims abstract description 39
- ANJAOCICJSRZSR-WAYWQWQTSA-N 9Z-Tetradecenal Chemical compound CCCC\C=C/CCCCCCCC=O ANJAOCICJSRZSR-WAYWQWQTSA-N 0.000 claims abstract description 25
- ANJAOCICJSRZSR-UHFFFAOYSA-N Z9-14:Ald Natural products CCCCC=CCCCCCCCC=O ANJAOCICJSRZSR-UHFFFAOYSA-N 0.000 claims abstract description 25
- SATICYYAWWYRAM-UHFFFAOYSA-N (2E,4E)-2,4-heptadienal Natural products CCC=CC=CC=O SATICYYAWWYRAM-UHFFFAOYSA-N 0.000 claims abstract description 19
- SATICYYAWWYRAM-VNKDHWASSA-N (E,E)-hepta-2,4-dienal Chemical compound CC\C=C\C=C\C=O SATICYYAWWYRAM-VNKDHWASSA-N 0.000 claims abstract description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 48
- 239000000203 mixture Substances 0.000 claims description 36
- 229940095068 tetradecene Drugs 0.000 claims description 25
- 229940069096 dodecene Drugs 0.000 claims description 16
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 12
- 241000256250 Spodoptera littoralis Species 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 description 53
- 238000009472 formulation Methods 0.000 description 17
- 208000009091 myxoma Diseases 0.000 description 12
- SATICYYAWWYRAM-DSSYRYGXSA-N (2E)-hepta-2,4-dienal Chemical compound CCC=C\C=C\C=O SATICYYAWWYRAM-DSSYRYGXSA-N 0.000 description 9
- 241001469654 Lawsonia <weevil> Species 0.000 description 9
- 241000607479 Yersinia pestis Species 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 241000255777 Lepidoptera Species 0.000 description 3
- 241000256259 Noctuidae Species 0.000 description 3
- 230000001617 migratory effect Effects 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000256248 Spodoptera Species 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010004716 Binge eating Diseases 0.000 description 1
- 208000032841 Bulimia Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001477928 Mythimna Species 0.000 description 1
- 241000618568 Mythimna loreyi Species 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000014679 binge eating disease Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P17/00—Pest repellants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a high-specificity spodoptera frugiperda sex attractant and application thereof. Among them, cis-9-tetradecenal and/or trans, trans-2, 4-heptadienal can improve spodoptera frugiperda specificity of spodoptera frugiperda, for example, they can reduce the number of spodoptera frugiperda armyworm.
Description
Technical Field
The invention relates to the field of biological control, in particular to a compound for reducing the quantity of spodoptera frugiperda sex pheromone induced by myxoplasma gondii and improving spodoptera frugiperda inducing specificity.
Background
Spodoptera frugiperda Spodoptera frugiperda (Smith), an alias fall armyworm, belongs to the genus spodoptera of the family Lepidoptera, the genus spodoptera, and the predatory of larvae, the host plant reaches more than 76 families 350, and the leaf, stem and growing point of the fed plant can cause serious losses to commercial crops such as corn, rice, sorghum, sugarcane, wheat, and the like, vegetable crops and cotton. Spodoptera frugiperda is originally in tropical and subtropical areas in america, mainly distributed in the united states, brazil, mexico, caribbean coasts and other areas, has strong migration capability and high migration speed, and since the invasion of the africa in 2016, the loss of up to 2 hundred million tons of local corn yield is caused, and the economic loss is up to 61 hundred million dollars. Quarantine pests were listed in europe in 2017 and advanced invasion in india, bangladesh, thailand, maine, sriland, 2018.
Sex pheromone is a trace chemical substance for the amphiprotic communication of insects, is released by gonads of female moths generally, has attraction effect on remote male moths, has species specificity, and can repel closely-related species male moths through own secondary sex pheromone components so as to achieve reproduction isolation. The sex attractant is an attractant based on sex pheromone, can trap a large amount of target male moths, monitor population and the like, and is widely used for preventing and controlling most of agriculture and forestry pests. The sex attractant is used for monitoring and early warning spodoptera frugiperda with serious agricultural hazard, and the premise of timely control is to grasp the occurrence dynamics of spodoptera frugiperda. At present, spodoptera frugiperda sex attractant products of a plurality of factories in China can lure spodoptera frugiperda, but have poor specificity, and can lure a large number of non-target species such as myxoma, and when the spodoptera frugiperda sex attractant products are used for population monitoring, prevention and control, the accuracy of insect condition forecast data can be influenced, so that wrong chemical prevention and control time is caused, and the phenomenon is similarly reported in northeast China in the United states and in the Okinawa county in Japan.
Mythimna rosea, mythimna loreyi (duponche), belonging to the genus Lepidoptera (Lepidoptera) noctuid (Noctuidae) and belonging to the genus noctuid, also belonging to the genus global migratory pest, larvae have omnivorous and binge eating properties, occur in the field in combination with spodoptera frugiperda, are ecologically highly overlapping, and are listed by the agricultural rural authorities as a list of crop pest and disease damage due to severe harm.
Disclosure of Invention
One of the present invention provides the use of cis-9-tetradecenal and/or trans, trans-2, 4-heptadienal to increase spodoptera frugiperda specificity of spodoptera frugiperda sex pheromone.
In a specific embodiment, the cis-9-tetradecenal and/or trans, trans-2, 4-heptadienal is capable of reducing the number of spodoptera frugiperda sex pheromones induced by myxoplasma gondii.
In a specific embodiment, the spodoptera frugiperda is an adult male spodoptera frugiperda.
In a specific embodiment, the myxoplasma gondii is male myxoplasma gondii adults.
The second invention provides a composition comprising spodoptera littoralis sex pheromone and cis-9-tetradecenal.
In a specific embodiment, the mass ratio of the spodoptera frugiperda sex pheromone and the cis-9-tetradecenal is (89.5 to 100): (1 to 15).
In a specific embodiment, the mass ratio of the spodoptera frugiperda sex pheromone and the cis-9-tetradecenal is (89.5 to 100): (5 to 15).
In a specific embodiment, trans-2, 4-heptadienal is also included in the composition.
In a specific embodiment, the spodoptera littoralis sex pheromone, the cis-9-tetradecenal and the trans, trans-2, 4-heptadienal are present in a mass ratio of (89.5 to 100): (1 to 15): (5 to 10).
In a specific embodiment, the spodoptera littoralis sex pheromone, the cis-9-tetradecenal and the trans, trans-2, 4-heptadienal are present in a mass ratio of (89.5 to 100): (5 to 15): (5 to 10).
In a specific embodiment, the spodoptera littoralis sex pheromone is cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate.
In one embodiment, the mass ratio of cis-9-tetradecene acetate, cis-7-dodecene acetate, and cis-11-hexadecene acetate is 79: (0.5 to 1): (10 to 20).
In a specific embodiment, a solvent is also included in the composition.
In one embodiment, the solvent is hexane.
The third aspect of the present invention provides a lure comprising a carrier and a composition according to any one of the second aspects of the present invention, wherein the composition is adsorbed onto the carrier.
In one embodiment, the carrier is a rubber stopper.
The invention has the beneficial effects that:
the invention discovers that cis-9-tetradecenal and the compound trans, trans-2, 4-heptadienal can not be used for repelling male spodoptera frugiperda by themselves, but after the cis-9-tetradecenal is added into spodoptera frugiperda sex pheromone, male spodoptera frugiperda can be repelled, the attraction to the male spodoptera frugiperda is obviously reduced, namely the attraction to non-target species is reduced, and the specificity or specificity of the spodoptera frugiperda is improved; the anti-2, 4-heptadienal is added into the composition of spodoptera frugiperda sex pheromone and cis-9-tetradecenal, so that the male myxoworm of the Lawsonia can be further repelled, the attraction to the myxoworm of the Lawsonia is further remarkably reduced, the attraction to non-target species is further reduced, and the specificity or specificity of attracting spodoptera frugiperda is improved. The result shows that the spodoptera frugiperda sex attractant is favorable for improving the population monitoring accuracy of the spodoptera frugiperda, which is an invasive pest with migratory property, so that the accurate prevention and control time is judged, and the spodoptera frugiperda sex attractant has important application value for preventing and controlling important agricultural pest spodoptera frugiperda.
Detailed Description
The above-described aspects of the invention are described in further detail below in the form of preferred embodiments, which are not to be construed as limiting the invention.
Reagents for use in the examples of the invention are commercially available unless otherwise specified.
Cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate are spodoptera frugiperda sex pheromone components.
Example 1
Test of the attracting effect of cis-9-tetradecenal on myxoma Latifolia
Test time and place: the planting crop in the field is summer corn in the county of Hui nationality of Yi nationality of mountain of white self-curing state of Yuan nan province of 15 days 6 to 27 days 6 months 2022.
The preparation method of the lure comprises the following steps: cis-9-tetradecene acetate, cis-7-dodecene acetate, cis-11-hexadecene acetate and cis-9-tetradecene aldehyde were prepared into mother solutions having a concentration of 10. Mu.g/mu.l, respectively, using n-hexane as a solvent.
100. Mu.l of N-hexane was added dropwise to a green rubber stopper (from Mitsubishi tetragonal biotechnology) of 240mm by 340mm size using N-hexane as a negative control, to prepare a lure, and this was designated as N-CK.
The formula of spodoptera littoralis sex pheromone is used as a positive control: 79 microliters of the mother liquor of 10 micrograms/microliter of cis-9-tetradecene acetate was taken, 0.5 microliters of the mother liquor of 10 micrograms/microliter of cis-7-dodecene acetate was taken, 10 microliters of the mother liquor of 10 micrograms/microliter of cis-11-hexadecene acetate was taken, the three were thoroughly mixed, and then the mixture was dropped onto a green rubber plug of 240mm×340mm size to prepare a lure, so that the total dose of the three was 0.895 milligrams, which was designated as P-CK-1.
The formulation of spodoptera littoralis sex pheromone was used as another positive control: 79 microliters of the mother liquor of the 10 micrograms/microliter cis-9-tetradecene acetate was taken, 1 microliter of the mother liquor of the 10 micrograms/microliter cis-7-dodecene acetate was taken, 20 microliters of the mother liquor of the 10 micrograms/microliter cis-11-hexadecene acetate was taken, the three were fully mixed, and then the mixture was dropped onto a green rubber plug of 240mm×340mm size to prepare a lure, so that the total dose of the three was 1 mg, which was designated as P-CK-2.
100. Mu.l of the 10. Mu.g/. Mu.l of cis-9-tetradecenal mother liquor was taken and dropped onto a green rubber stopper having a size of 240 mm. Times.340 mm to prepare a lure, so that the dose of cis-9-tetradecenal was 1 mg, which was designated as L1.
79. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of cis-9-tetradecene acetate was taken out, 0.5. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of cis-7-dodecene acetate was taken out, 10. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of cis-11-hexadecene acetate was taken out, 0.3. Mu.l of the mother liquor of 10. Mu.g/. Mu.l of cis-9-tetradecene aldehyde was taken out, and the four were thoroughly mixed and then dropped onto a green rubber plug of 240 mm. Times.340 mm in size to prepare a lure, so that the total dose of the four was 0.898 mg, which was designated as L2.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 0.5 microliters from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 10 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 1 microliter from the 10 micrograms/microliter of cis-9-tetradecene aldehyde mother liquor, four were thoroughly mixed, and then dropped onto a 240mm×340mm sized green rubber plug to make a lure, so that the total dose of the four is 0.905 milligrams, designated as L3.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 0.5 microliters from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 10 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 5 microliters from the 10 micrograms/microliter of cis-9-tetradecene aldehyde mother liquor, four were thoroughly mixed, and then dropped onto a 240mm×340mm sized green rubber plug to make a lure, so that the total dose of the four is 0.945 milligrams, designated as L4.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 0.5 microliters from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 10 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 15 microliters from the 10 micrograms/microliter of cis-9-tetradecene aldehyde mother liquor, four were thoroughly mixed, and then dropped onto a green rubber plug of 240mm×340mm size to prepare a lure, so that the total dose of the four is 1.045 milligrams, denoted as L5.
79 microliters of the mother liquor of 10 micrograms/microliter of cis-9-tetradecene acetate was taken, 1 microliter of the mother liquor of 10 micrograms/microliter of cis-7-dodecene acetate was taken, 20 microliters of the mother liquor of 10 micrograms/microliter of cis-11-hexadecene acetate was taken, 0.3 microliter of the mother liquor of 10 micrograms/microliter of cis-9-tetradecene aldehyde was taken, four were thoroughly mixed, and then the mixture was dropped onto a green rubber plug of 240mm×340mm size to prepare a lure, so that the total dose of the four was 1.003 milligrams, which was designated as L6.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 1 microliter from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 20 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 1 microliter from the 10 micrograms/microliter of cis-9-tetradecene aldehyde mother liquor, four are fully mixed, and then the mixture is dripped on a green rubber plug with the size of 240mm multiplied by 340mm to prepare a lure, so that the total dosage of the four is 1.01 milligrams, and the lure is marked as L7.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 1 microliter from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 20 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 5 microliters from the 10 micrograms/microliter of cis-9-tetradecene aldehyde mother liquor, and four are thoroughly mixed and then added dropwise to a green rubber plug of 240mm×340mm size to prepare a lure, so that the total dosage of the four is 1.05 milligrams, which is designated as L8.
79 microliters from the 10 micrograms/microliter of cis-9-tetradecene acetate mother liquor, 1 microliter from the 10 micrograms/microliter of cis-7-dodecene acetate mother liquor, 20 microliters from the 10 micrograms/microliter of cis-11-hexadecene acetate mother liquor, 15 microliters from the 10 micrograms/microliter of trans-cis-9-tetradecene aldehyde mother liquor, four are fully mixed, and then the mixture is dropped onto a green rubber plug with the size of 240mm multiplied by 340mm to prepare a lure, so that the total dosage of the four is 1.15 milligrams, which is denoted as L9.
The positive control, negative control, and components and amounts of L1 to L9 are shown in table 1.
TABLE 1
Test fields of 20 mu are selected and divided into 12 treatment groups, and each treatment group is repeated for 3. The traps were 20 cm above the maize plants, each lure was individually placed in one trap device with 15 meters spacing between each trap, and 3 rows were placed, with a total of 12 different treated lures per row randomly placed P-CK-1, P-CK-2, N-CK, L1 through L9. The trapping amount of spodoptera frugiperda and myxoplasma gondii adults and the sex of the trapped adults in the trap were counted once a day, after which the trapped adults were emptied from the trap, and then the positions of the different treatment traps in each row were randomly exchanged to eliminate test errors. The total insect trapping amount of the single lure in 13 days of the 2022 6-27-day hanging period was counted under 3 replicates, the average value of the 3 replicates was calculated, the significant difference was analyzed by one-way ANOVA, the LSD was used for comparison, and the result was shown in table 2, with α=0.05.
TABLE 2
Note that: capital letters a-D represent significant differences in spodoptera frugiperda trapping amounts between the different treatment groups; the lowercase letters a-e represent significant differences in the amount of myxoplasma gondii trapped between the different treatment groups.
From the results of Table 1, it was found that the N-CK lure and the L1 lure were unable to lure Spodoptera frugiperda male adults and myxoma Latifolium male adults; the quantity of male adults of spodoptera frugiperda induced by the P-CK-1 induced core, the L2 induced core, the L3 induced core and the L4 induced core is equal, the quantity of male adults of myxoplasma gondii induced by the P-CK-1 induced core and the L2 induced core is equal, and the quantity of male adults of myxoplasma gondii induced by the L3 induced core, the L4 induced core and the L5 induced core is remarkably reduced; the P-CK-2 lure, the L6 lure, the L7 lure and the L8 lure are equivalent in number of male adults of spodoptera frugiperda, the P-CK-2 lure and the L6 lure are equivalent in number of male adults of myxoworm of Lawsonia, and the L7 lure, the L8 lure and the L9 lure are remarkably reduced in number of male adults of myxoworm of Lawsonia.
The above results fully demonstrate that cis-9-tetradecenal alone has no behavioral effect on male adults of myxoworm, and that the mass ratio of spodoptera frugiperda sex pheromone to cis-9-tetradecenal is (89.5 to 100): in the process of (1-15), cis-9-tetradecenal can remarkably reduce the trapping effect of spodoptera frugiperda sex pheromone on male adults of myxoma roulette, has little influence on trapping spodoptera frugiperda, and improves the specificity of spodoptera frugiperda sex attractant and the accuracy of population monitoring.
Example 2
Test of the attracting effect of trans, trans-2, 4-heptadienal on myxoma Latifolium
Test time and place: the planting crop in the field is summer corn, and the year is from 29 days in 2022, 6 months to 11 days in 7 months, and the white self-curing state of Yunnan, the Yi nationality county of Hui nationality of mountain.
The preparation method of the lure comprises the following steps: the trans, trans-2, 4-heptadienal was prepared as a mother liquor having a concentration of 10. Mu.g/. Mu.l using n-hexane as a solvent.
From the above 10. Mu.g/μl of trans, trans-2, 4-heptadienal mother liquor, 100 μl was taken and dropped onto a 240mm×340mm size green rubber plug to prepare a lure, so that the dose of trans, trans-2, 4-heptadienal was 1 mg, designated as L10.
The formulation of L3 in example 1 was formulated.
To the L3 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal was added, 1. Mu.l was thoroughly mixed, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, so that the total dose of the five was 0.915 mg, designated as L11.
To the L3 formulation of example 1, 10 micrograms/microliter of trans, trans-2, 4-heptadienal mother liquor 5 microliter was added, thoroughly mixed, and then dropped onto a 240mm by 340mm sized green rubber plug to make a lure, giving a total dosage of 0.955 milligrams for the five, designated L12.
To the L3 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal, 10 μl was added, mixed thoroughly, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, so that the total dose of the five was 1.005 mg, designated as L13.
The formulation of L5 in example 1 was formulated.
To the L5 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal was added, 1. Mu.l was thoroughly mixed, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, so that the total dose of the five was 1.055 mg, designated as L14.
To the L5 formulation of example 1, 10. Mu.g/μl of trans, trans-2, 4-heptadienal mother liquor 5 μl was added, thoroughly mixed, and then dropped onto a 240mm by 340mm sized green rubber plug to prepare a lure, giving a total dosage of 1.095 mg for the five, designated L15.
To the L5 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal, 10 μl was added, mixed thoroughly, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, giving a total dosage of 1.145 mg for the five, designated L16.
The formulation of L7 in example 1 was formulated.
To the L7 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal was added, 1. Mu.l was thoroughly mixed, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, so that the total dose of the five was 1.02 mg, designated as L17.
To the L7 formulation of example 1, 10 micrograms/microliter of trans, trans-2, 4-heptadienal mother liquor 5 microliter was added, thoroughly mixed, and then dropped onto a 240mm by 340mm sized green rubber plug to make a lure, giving a total dosage of 1.06 milligrams of the five, designated as L18.
To the L7 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal, 10 μl was added, mixed well, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, giving a total dosage of five of 1.11 mg, designated as L19.
The formulation of L9 in example 1 was formulated.
To the L9 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal was added, 1. Mu.l was thoroughly mixed, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, so that the total dose of the five was 1.16 mg, designated as L20.
To the L9 formulation of example 1, 10. Mu.g/μl of trans, trans-2, 4-heptadienal mother liquor 5 μl was added, thoroughly mixed, and then dropped onto a 240mm by 340mm sized green rubber plug to prepare a lure, giving a total dosage of 1.2 mg for the five, designated L21.
To the L9 formulation of example 1, 10. Mu.g/μl of the mother liquor of trans-2, 4-heptadienal, 10 μl was added, mixed well, and then dropped onto a green rubber stopper of 240mm by 340mm size to prepare a lure, giving a total dosage of five of 1.25 mg, designated as L22.
The compositions and contents of the different treatment cores L3, L5, L7, L9 to L22 are shown in Table 3.
TABLE 3 Table 3
The test fields were selected at 20 mu and divided into 17 treatment groups, each treatment group being repeated 3 times.
Otherwise, the same as in example 1 was conducted.
The experimental results are shown in Table 4.
TABLE 4 Table 4
Note that: capital letters a-E represent significant differences in spodoptera frugiperda trapping amounts between the different treatment groups; the lowercase letters a-d represent significant differences in the amount of myxoplasma gondii trapped between the different treatment groups.
From the results of table 4, L10 lures no spodoptera frugiperda and male adults of myxoplasma gondii; the quantity of male adults of spodoptera frugiperda induced by the L3 lure, the L11 lure, the L12 lure and the L13 lure is equal, the quantity of male adults of myxoma rouxii induced by the L3 lure and the L11 lure is equal, the quantity of male adults of myxoma rouxii induced by the L12 lure and the L13 lure is obviously reduced, and the quantity of the male adults of myxoma rouxii induced by the L13 lure is relatively lowest; the quantity of male adults of spodoptera frugiperda induced by the L5 lure, the L14 lure, the L15 lure and the L16 lure is equal, the quantity of male adults of myxoworm of Lawsonia induced by the L5 lure and the L14 lure is equal, the quantity of male adults of myxoworm of Lawsonia induced by the L15 lure and the L16 lure is obviously reduced, and the quantity of the male adults of myxoworm of Lawsonia induced by the L16 lure is relatively lowest; the quantity of male adults of spodoptera frugiperda induced by the L7 lure, the L17 lure, the L18 lure and the L19 lure is equal, the quantity of male adults of myxoma rouxii induced by the L7 lure and the L17 lure is equal, the quantity of male adults of myxoma rouxii induced by the L18 lure and the L19 lure is obviously reduced, and the quantity of the male adults of myxoma rouxii induced by the L19 lure is relatively lowest; the L9 lure, the L20 lure, the L21 lure and the L22 lure are equivalent in number of male adults of spodoptera frugiperda, the L9 lure and the L20 lure are equivalent in number of male adults of myxoworm of Lawsonia, the L21 lure and the L22 lure are remarkably reduced in number of male adults of myxoworm of Lawsonia, and the L22 lure is relatively lowest.
The above results fully demonstrate that trans, trans-2, 4-heptadienal alone has no behavioral effect on male adults of myxoworm of Latifolia, when spodoptera frugiperda sex pheromone, cis-9-tetradecenal and trans, trans-2, 4-heptadienal are in a mass ratio of (89.5 to 100): (1 to 15): and (5) in the process (10), the trans, trans-2, 4-heptadienal can obviously reduce the attracting effect of the composition of spodoptera frugiperda sex pheromone and cis-9-tetradecenal on male adults of myxoma roursonii, further improve the specificity of the spodoptera frugiperda sex attractant and realize the accurate monitoring, prevention and control of the spodoptera frugiperda population of the migratory insect pest cordyceps sinensis.
Claims (10)
1. Use of cis-9-tetradecenal for increasing spodoptera frugiperda specificity, wherein the spodoptera frugiperda sex pheromone is 79: (0.5 to 1): cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate of (10 to 20); the mass ratio of the spodoptera littoralis sex pheromone and the cis-9-tetradecenal is (89.5 to 100): (1 to 5).
2. The use according to claim 1, wherein said cis-9-tetradecenal is capable of reducing the number of spodoptera frugiperda sex pheromones induced by myxoplasma gondii.
3. The use according to claim 1, wherein the spodoptera frugiperda is an adult male spodoptera frugiperda.
4. The use according to claim 2, wherein the myxoworm is a male myxoworm strain adult.
5. A composition comprising spodoptera littoralis sex pheromone and cis-9-tetradecenal, the spodoptera littoralis sex pheromone being in a mass ratio of 79: (0.5 to 1): cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate of (10 to 20); the mass ratio of the spodoptera littoralis sex pheromone and the cis-9-tetradecenal is (89.5 to 100): (1 to 5).
6. The composition of claim 5, further comprising trans, trans-2, 4-heptadienal; the mass ratio of the spodoptera frugiperda sex pheromone, the cis-9-tetradecenal and the trans, trans-2, 4-heptadienal is (89.5 to 100): (1 to 5): (5 to 10).
7. The composition of any one of claims 5 or 6, further comprising a solvent.
8. The composition of claim 7, wherein the solvent is hexane.
9. A lure comprising a carrier and a composition according to any one of claims 5 to 8, wherein the composition is adsorbed onto the carrier.
10. The lure according to claim 9, wherein the carrier is a rubber plug.
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Non-Patent Citations (2)
| Title |
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| Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China;Wang chan等;《Pest Management Science》;第78卷(第7期);第2995-3004页 * |
| 昆虫性信息素及其类似物干扰昆虫行为的机理和应用研究进展;王安佳;张开心;梅向东;高玉林;张涛;折冬梅;宁君;;农药学学报(第04期);第425-438页 * |
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