CN115436617A - Dry analytical reagent for gamma-glutamyl transpeptidase detection - Google Patents
Dry analytical reagent for gamma-glutamyl transpeptidase detection Download PDFInfo
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Abstract
A dry analytical reagent for detecting gamma-glutamyl transpeptidase comprises an upper support layer, a diffusion layer, a reagent layer, a support and a lower support layer from top to bottom in sequence, wherein the diffusion layer, the reagent layer and the support are arranged at the middle position between the upper support layer and the lower support layer from top to bottom in sequence, the pores at the two ends of the upper support layer and the lower support layer are bonded through the middle support layer, the middle positions of the upper support layer and the lower support layer are respectively provided with a sample adding hole and a test hole, and the porosity of the diffusion layer is 40-75%; the diffusion layer consists of high-crosslinked polymethyl methacrylate microspheres, an adhesive and a surfactant, and the particle size of the high-crosslinked polymethyl methacrylate microspheres is 5-50 mu m; the reagent layer comprises a surfactant, diglycine, L-gamma-glutamyl-3-carboxyl-p-nitroaniline, a buffer solution, a stabilizer and a water-soluble high polymer. The diffusion layer has the characteristic of isotropy, has good detection specificity, high accuracy and wide measurement range, meets the requirement of instant diagnosis and has great clinical application value.
Description
Technical Field
The invention relates to a dry analytical reagent for detecting gamma-glutamyl transpeptidase, belonging to the field of clinical diagnosis.
Background
Gamma-glutamyl transferase (GGT) is a peptidase that catalyzes the gamma-glutamyl transferase reaction. Serum GGT is a sensitive index of hepatobiliary diseases, and the serum GGT can be increased in hepatobiliary diseases caused by various reasons. GGT clinical detection is divided into a wet chemical method and a dry chemical method, wherein the wet chemical method is a common analysis method, the reagent preparation period of the wet chemical method is long, the operation of professionals is needed, and the detection result is easily influenced by the level of the operators, while the dry chemical analysis method has the advantages of high detection speed, easy storage of detection reagents, no need of any liquid reagent, low detection threshold requirement, strong occasion adaptability and the like, along with the improvement of the requirement of emergent emergency detection, the report time of biochemical analysis in clinic provides a shorter requirement, and the development of the biochemical detection technology in emergency treatment from the traditional wet chemistry to the dry biochemistry is promoted gradually. The dry chemical analysis method has high detection speed and high detection automation level, can carry out personalized project combination detection on scattered samples at any time and any place, and is particularly suitable for scenes such as emergency treatment, primary clinics, blood stations, physical examination mechanisms, examination laboratories and the like.
At present, dry analytical reagents have been used to continue the coating technique of photosensitive films, in which functional layers such as diffusion layers, light blocking layers, and reagent layers are sequentially coated on a support, and the concentration of an analyte is analyzed by measuring the change in the density of reflected light. The multilayer film dry sheet has accurate test result, but the production process is complex, the production of the diffusion layer needs to use a plurality of organic solvents, a large amount of organic waste gas is generated, the environment is harmful, and the danger of the production process is increased. Another type of dry chemical assay reagent consists of a plastic support layer, a reaction area at one end comprising a sample layer and a reagent layer, the reagent layer using a fabric as a reagent carrier, and the sample application and the test are both performed on the upper part of the reagent layer.
Patent CN201910219761.9 discloses an automatic blood filtration dry-type photochemical method micro-channel liver function three-item detection card, wherein a blood filtration membrane adopts a commercially available product, and a transmission photometry is adopted for testing, so that the substance interference in a sample to be detected cannot be completely avoided. Patent CN201910236059.3 discloses a reagent for measuring γ -glutamyl transferase and a diagnostic analysis method, wherein the diagnostic analysis method has more complicated steps, still needs to be operated by a person with certain professional knowledge, and the precision of the detection result of the microfluidic chip cannot be guaranteed.
Disclosure of Invention
The invention provides a dry analytical reagent for detecting gamma-glutamyl transpeptidase, wherein the diffusion layer is non-swelling and non-permeable high cross-linked polymethyl methacrylate microsphere, which is combined with a small amount of specific adhesive to form a diffusion layer with high porosity structure, which can realize high-efficiency and rapid permeation and diffusion of the liquid to be analyzed containing gamma-glutamyl transpeptidase, and can uniformly distribute, diffuse and rapidly transfer the sample to be analyzed containing analytes in the interior.
The technical scheme adopted by the invention for solving the technical problem is as follows:
the dry analytical reagent for detecting the gamma-glutamyl transpeptidase comprises an upper support layer, a diffusion layer, a reagent layer, a support and a lower support layer from top to bottom in sequence, wherein the diffusion layer, the reagent layer and the support are arranged at the middle position between the upper support layer and the lower support layer from top to bottom in sequence, pores at two ends of the upper support layer and the lower support layer are bonded through the middle support layer, a sample adding hole and a test hole are respectively arranged at the middle positions of the upper support layer and the lower support layer, the diffusion layer is an isotropic porous diffusion membrane, and the porosity of the diffusion layer is 40-75%; the diffusion layer consists of highly-crosslinked polymethyl methacrylate microspheres, an adhesive and a surfactant, the particle size of each highly-crosslinked polymethyl methacrylate microsphere is 5-50 mu m, the adhesive is concentrated on the surface of each highly-crosslinked polymethyl methacrylate microsphere and the adjacent area of the adjacent microsphere, and the highly-crosslinked polymethyl methacrylate microspheres are bonded into a coherent three-dimensional lattice; the reagent layer comprises a surfactant, diglycine, L-gamma-glutamyl-3-carboxyl-p-nitroaniline, a buffer solution, a stabilizer and a water-soluble high polymer.
The dry analytical reagent for detecting gamma-glutamyl transpeptidase is prepared by suspension polymerization, the cross-linking agent is one or a composition of more than two of methacrylate, ethylene glycol dimethacrylate, urethane, divinylbenzene and N, N-methylene bisacrylamide, and the mass of the cross-linking agent accounts for 5-30 wt% of the mass of methyl methacrylate MMA monomer.
The dry analytical reagent for detecting gamma-glutamyl transpeptidase is characterized in that the binder comprises one of poly (butyl acrylate-styrene), poly (N-butyl acrylate-acrylic acid), polyethylacrylate, poly (N-isopropylacrylamide), poly (ethyl acrylate-styrene), poly (butyl acrylate-methacrylic acid), poly (N-butyl methacrylate), poly (methyl methacrylate-butyl acrylate) or poly (vinyl toluene-tert-butyl styrene-methacrylic acid).
In the dry analytical reagent for detecting gamma-glutamyl transpeptidase, the addition amount of the high cross-linked polymethyl methacrylate microspheres per unit square meter is 20-500g, and the addition amount of the adhesive per unit square meter accounts for 1.5-4.5% of the addition amount of the high cross-linked polymethyl methacrylate microspheres per unit square meter.
The surface active agent is a nonionic surface active agent and comprises one of p-octyl phenoxy polyoxyethylene ether, p-nonyl phenoxy polyoxyethylene ether, polyoxyethylene sorbitan monolaurate or octyl glucoside.
In the above dry analytical reagent for detecting γ -glutamyl transpeptidase, the surfactant is preferably a nonionic surfactant having a chain structure consisting of 8 to 15 oxyethylene or oxypropylene groups linked to each other.
The above dry analytical reagent for detecting gamma-glutamyl transpeptidase, wherein the buffer is a pH buffer with a pH value in the range of 7.0 to 9.0, and the buffer contains tris (hydroxymethyl) aminomethane (tris), phosphate, borate, lysine; one of a sodium salt or a potassium salt of N, N-bis (2-hydroxyethyl) glycine, N-2-hydroxyethylpiperazine-N ' -2-hydroxypropane-3-sulfonic acid, a sodium salt or a potassium salt of N-2-hydroxyethylpiperazine-N ' -3-sulfonic acid, a sodium salt or a potassium salt of N- [ tris (hydroxymethyl) methyl ] -3-aminopropane sulfonic acid and a sodium salt or a potassium salt of N-2-hydroxyethylpiperazine-N ' -2-ethanesulfonic acid, a sodium salt or a potassium salt of 1, 4-piperazine-diethylsulfonic acid (PIPES), and an acid, a base, or a salt in combination with any one of the above compounds.
The dry analytical reagent for detecting gamma-glutamyl transpeptidase is characterized in that the stabilizer is one or more of polysaccharide and disaccharide; the water-soluble high molecular polymer is one or a mixture of more of gelatin, gelatin derivatives, agarose, dextran, polyvinyl alcohol, polyacrylamide and hydrophilic cellulose derivatives.
The dry analytical reagent for detecting gamma-glutamyl transpeptidase is characterized in that the upper support, the lower support and the middle support are all transparent plastic substrates with light transmittance of more than 80% under the wavelength of 200nm-900nm, and the transparent plastic substrates comprise one of polyethylene glycol terephthalate, polycarbonate, polypropylene and polyethylene, and the thickness of the transparent plastic substrates is 50-300 mu m.
The invention has the beneficial effects that:
the preparation process of the dry analytical reagent for detecting the gamma-glutamyl transpeptidase does not use organic solvent, and the production and preparation process is environment-friendly; the diffusion layer is made of water-insoluble and impermeable high-crosslinking polymethyl methacrylate microspheres, and is combined with a very small amount of specific adhesive to form the diffusion layer with a high porosity structure, so that the liquid to be analyzed containing the gamma-glutamyl transpeptidase can be efficiently and quickly permeated and diffused, and a sample to be detected containing an analyte can be uniformly distributed, diffused and quickly transferred in the diffusion layer; the L-gamma-glutamyl-3-carboxyl-p-nitroaniline with good water solubility is used as a chromogenic substrate, the accuracy of a test result is high, the measurement range is wide, the requirements of instant diagnosis and field diagnosis are met, and the clinical application value is high.
Drawings
FIG. 1 is a schematic view of a multilayer film dry chemical reagent sheet according to the present invention;
FIG. 2 is a graph showing the correlation between the method of the present invention of example 1 and a plurality of test samples using the γ -glutamylcarboxylanilide control method;
FIG. 3 is a graph of the correlation of a plurality of test samples according to the method of the present invention and using the gamma-glutamylcarboxylanilide control of example 2;
FIG. 4 is a graph showing the correlation between the method of the present invention of example 3 and a plurality of test samples using the gamma-glutamylcarboxylanilide control method.
In the figure: 1. an upper support layer; 1-1, sample adding holes; 2. a diffusion layer; 3. a reagent layer; 4. a support; 5. a lower support layer; 5-1, testing holes; 6. an intermediate support layer.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
The dry analytical reagent of the present invention enables the determination of gamma-glutamyl transpeptidase in a body fluid sample. The body fluid can be blood or urine, etc.; the body fluid sample may be blood or urine as it is, or may be a sample subjected to an appropriate pretreatment.
The dry type analytical reagent comprises an upper support layer 1, a diffusion layer 2, a reagent layer 3, a support 4 and a lower support layer 5 from top to bottom in sequence, wherein the diffusion layer, the reagent layer and the support are arranged at the middle position between the upper support layer and the lower support layer from top to bottom in sequence, pores at two ends of the upper support layer and the lower support layer are bonded through the middle support layer 6, a sample adding hole 1-1 and a test hole 5-1 are respectively arranged at the middle positions of the upper support layer 1 and the lower support layer 5, liquid to be detected is dripped onto the diffusion layer through the sample adding hole, the liquid to be detected is quickly and uniformly diffused in four directions through the diffusion layer and is longitudinally diffused towards the reagent layer, biochemical reaction is generated in the reagent layer, the support is a structure layer with a supporting function and a light-transmitting carrier, and the upper support layer, the lower support layer and the middle support layer are inner packaging structures with functions of fixing and protecting the support distributed with the reagent layer and the diffusion layer.
The diffusion layer is a diffusion layer which is formed by combining non-swelling and impermeable high-crosslinked polymethyl methacrylate microspheres when meeting water with a very small amount of specific adhesive to form a high-porosity structure, the adhesive is concentrated on the surface of the high-crosslinked polymethyl methacrylate microspheres and adjacent areas of adjacent microspheres to bond the high-crosslinked polymethyl methacrylate microspheres into a coherent three-dimensional lattice, the three-dimensional lattice structure shows high void volume, and the particle size distribution of the high-crosslinked polymethyl methacrylate microspheres and the addition amount of the adhesive are controlled to form various uniform porous diffusion layers with different porosities and pore size distributions, so that various analytes can be quickly absorbed, uniformly distributed, metered and quickly transmitted, and the substances to be detected with different molecular weight distributions and compositions in biological liquid can be analyzed.
The high-crosslinking polymethyl methacrylate microsphere is prepared by a suspension polymerization technology, in order to meet the requirements of no swelling and impermeability of the polymer microsphere, a proper crosslinking agent is required to be added during the polymerization of the microsphere so as to obtain a high-strength crosslinking structure, the crosslinking agent in the invention is preferably divinylbenzene, and the addition amount of the crosslinking agent is preferably 5-2 wt% of the mass of methyl methacrylate MMA monomer; the particle size of the high cross-linked polymethyl methacrylate microsphere is controlled to be 5-50 mu m by controlling the reaction time, the addition amount of the high cross-linked polymethyl methacrylate microsphere is 20-500g per unit square meter, preferably 50-300g per unit square meter, the addition amount of the adhesive per unit square meter accounts for 1.5-4.5% of the addition amount of the high cross-linked polymethyl methacrylate microsphere, the porosity of the diffusion layer is controlled to be 40-75%, a large number of diffusion passages from hundreds of nanometers to several micrometers can be formed, the relative molecular mass of gamma-glutamyl transpeptidase is about 90kDa, the molecular size is about tens of nanometers, the diffusion passages of the diffusion layer can allow water, small molecular substances and gamma-glutamyl transpeptidase to be rapidly distributed, metered and transmitted, and macromolecular substances such as red blood cells and the like are prevented from passing through, so that effective filtration effect is formed.
The chromogenic substrate in the reagent layer is L-gamma-glutamyl-3-carboxyl-p-nitroaniline with good water solubility. The existing commonly used gamma-glutamyltranspeptidase detection kit basically adopts L-gamma-glutamyl-p-nitroaniline as a chromogenic substrate, but the L-gamma-glutamyl-p-nitroaniline has the defects of poor water solubility, low chromogenic density and the like, is difficult to reach the concentration of a saturated substrate, and cannot show the maximum activity of the L-gamma-glutamyltransferase. Through screening, the invention uses a substrate L-gamma-glutamyl-3-carboxyl-p-nitroaniline with good water solubility as a color developing substrate. The addition amount of L-gamma-glutamyl-3-carboxy-p-nitroaniline is 0.01-1g per unit square meter, preferably 0.02-0.5g per unit square meter.
The addition amount of the surfactant in the reagent layer is 1-25g per unit square meter, preferably 2-15g per unit square meter; 0.1-10g of diglycine per unit square meter, preferably 0.2-5g per unit square meter; the addition amount of the stabilizer is 0.2-10g per unit square meter; the water-soluble high molecular polymer is 1 to 50g per square meter, preferably 2 to 25g per square meter.
The preparation method of the dry type analytical reagent comprises the following steps:
(1) The reagent layer reagent solution is coated on the support by using a known method, preferably wire rod coating, blade coating and extrusion coating, wherein the wet film thickness of the reagent layer is preferably 50-500 μm, the reagent layer is dried at the temperature of 10-60 ℃, and the dry film thickness of the reagent layer is preferably 5-50 μm.
(2) The slurry for the diffusion layer is coated on the reagent layer (1) by a known method, preferably wire rod coating, blade coating, extrusion coating, the wet film thickness of the diffusion layer is preferably 60-600 μm, and the diffusion layer is dried at 10-60 deg.C, and the dry film thickness of the diffusion layer is preferably 20-200 μm.
(3) Cutting the material prepared in the step (2) into 1.2cm 2 The dry analytical reagent was immobilized and assembled by using a custom-made white PET plastic sheet as an upper support layer, a lower support layer and an intermediate support layer.
The detection method of the dry analytical reagent for detecting the gamma-glutamyl transpeptidase comprises the following steps:
dripping about 10 mu L of liquid to be detected on each homogeneous porous diffusion layer, incubating for 5 minutes at the temperature of about 37 ℃, and allowing the liquid to be detected to diffuse longitudinally towards the reagent layer while rapidly and uniformly diffusing in four directions through the diffusion layer to form uniform concentration distribution. GGT catalyzes partial transfer of glutamate of L-gamma-glutamyl-p-nitroaniline into glycylglycine and simultaneously generates p-nitroaniline. Reflection density detection was carried out by a rate method from the support side for the color change reaction generated by the dry analytical reagent, and the concentration of GGT in the liquid to be examined was calculated using a calibration curve prepared in advance. The equation for the reaction of the target substance GGT with the corresponding substance in the dry analytical reagent is as follows:
the preparation process of the dry analytical reagent for detecting the gamma-glutamyl transpeptidase does not need an organic solvent, is environment-friendly, and has good stability, easy storage and preservation, high detection accuracy and wide linear range.
The present invention will be further described with reference to the following examples.
Example 1
The reagent layer coating liquid having the composition shown in Table 1 below was applied to a colorless transparent 175 μm PET film support 4 and dried at a constant temperature of 40 ℃ to a thickness of 15 μm after drying. Subsequently, a diffusion layer coating liquid having the composition shown in Table 2 below was applied on the above coating layer and dried at a constant temperature of 35 ℃ so that the thickness after drying became 100. Mu.m. Drying, and cutting into 1.2cm pieces 2 Then white PET plastic sheets were used as the upper support layer 1, the lower support layer 5 and the intermediate support layer 6, and the assembly was ready for measurement.
TABLE 1 reagent layer formulation
| Reagent | Coating weight/m 2 |
| Gelatin | 10g |
| Double-glycylglycine | 1.8g |
| L-gamma-glutamyl-3-carboxy-p-nitroanilide | 200mg |
| PIPES buffer | pH7.7 |
| Triton X-100 | 1.5g |
| Sucrose | 1.2g |
TABLE 2 diffusion layer formulation
| Material | Coating weight/m 2 |
| Highly crosslinked polymethyl methacrylate microspheres (10 μm) | 150g |
| Poly (n-butyl acrylate-acrylic acid) emulsion | 3g |
| Surface active agent | 1.5g |
The correlation of multiple test samples was analyzed for 25 clinical serum specimens for the method of the invention and using a modified gamma glutamylcarboxylanilide control. As a result, as shown in fig. 2, the present invention can obtain a good correlation as compared with the reference method.
Example 2
The reagent layer coating liquid composed of the following table 3 was applied on the colorless transparent 175 μm PET film support 4 and dried at a constant temperature of 37 ℃ to a thickness of 10 μm after drying. Subsequently, a diffusion layer coating liquid having the composition of the following table 4 was applied on the above coating layer and dried at a constant temperature of 40 ℃ so that the thickness after drying became 150 μm. Drying, and cutting into 1.2cm pieces 2 Then white PET plastic sheets were used as the upper support layer 1, the lower support layer 5 and the intermediate support layer 6, and the assembly was ready for measurement.
TABLE 3 reagent layer formulation
| Reagent | Coating weight/m 2 |
| Gelatin | 10g |
| Diglycine | 3.5g |
| L-gamma-glutamyl-3-carboxy-p-nitroaniline | 150mg |
| PIPES buffer solution | pH8.0 |
| Triton X-100 | 1.5g |
| Sucrose | 2.0g |
TABLE 4 diffusion layer formulation
| Material | Coating weight/m 2 |
| Highly crosslinked polymethyl methacrylate microspheres (30 μm) | 100g |
| Poly (butyl acrylate-styrene) emulsion | 1.5g |
| Surface active agent | 1g |
The correlation of multiple test samples was analyzed for 25 clinical serum samples for the method of the invention and using a modified gamma glutamylcarboxylanilide control. The results are shown in fig. 3, and the present invention can obtain a good correlation compared with the reference method.
Example 3
The reagent layer coating liquid having the composition shown in Table 5 below was applied to a colorless transparent 175 μm PET film support 4 and dried at a constant temperature of 40 ℃ to a thickness of 20 μm after drying. Next, a diffusion layer coating solution having the composition shown in Table 6 below was applied on the above coating layer and dried at a constant temperature of 45 ℃ to allow the coating layer to standThe thickness after drying reached 100 μm. Drying, and cutting into 1.2cm 2 Then white PET plastic sheets were used as the upper support layer 1, the lower support layer 5 and the intermediate support layer 6, and the assembly was ready for measurement.
TABLE 5 reagent layer formulation
| Reagent | Coating weight/m 2 |
| Gelatin | 10g |
| Double-glycylglycine | 1.0g |
| L-gamma-glutamyl-3-carboxy-p-nitroaniline | 100mg |
| Tris buffer | pH7.8 |
| Triton X-100 | 1.5g |
| Sucrose | 0.6g |
TABLE 6 diffusion layer formulation
| Material | Coating weight/m 2 |
| Highly crosslinked polymethylmethacrylate microsphere (45 μm) | 150g |
| Poly (butyl acrylate-methacrylic acid) emulsion | 4g |
| Surface active agent | 2g |
The correlation of multiple test samples was analyzed for 25 clinical serum samples for the method of the invention and using a modified gamma glutamylcarboxylanilide control. As a result, as shown in fig. 4, the present invention can obtain a good correlation as compared with the reference method.
The above embodiments are only for illustrating the technical idea and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to follow the fact, and not to limit the protection scope of the present invention. All equivalent substitutions or modifications made according to the spirit of the present invention should be included in the scope of the claims of the present invention.
Claims (9)
1. A dry analytical reagent for detecting gamma-glutamyl transpeptidase, the dry analytical reagent comprises an upper support layer (1), a diffusion layer (2), a reagent layer (3), a support (4) and a lower support layer (5) from top to bottom in sequence, the diffusion layer, the reagent layer and the support are arranged at the middle position between the upper support layer and the lower support layer from top to bottom in sequence, the pores at the two ends of the upper support layer and the lower support layer are bonded through the middle support layer (6), and the middle position of the upper support layer (1) and the lower support layer (5) is respectively provided with a sample adding hole (1-1) and a test hole (5-1), the dry analytical reagent is characterized in that: the diffusion layer is an isotropic porous diffusion film, and the porosity of the diffusion layer is 40-75%; the diffusion layer consists of high-crosslinked polymethyl methacrylate microspheres, an adhesive and a surfactant, the particle size of the high-crosslinked polymethyl methacrylate microspheres is 5-50 mu m, the adhesive is concentrated on the surface of the high-crosslinked polymethyl methacrylate microspheres and the area adjacent to the adjacent microspheres, and the high-crosslinked polymethyl methacrylate microspheres are bonded into a coherent three-dimensional lattice; the reagent layer (3) comprises a surfactant, diglycine, L-gamma-glutamyl-3-carboxyl-p-nitroaniline, a buffer solution, a stabilizer and a water-soluble high polymer.
2. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 1, characterized in that: the high-crosslinking polymethyl methacrylate microspheres are prepared by a suspension polymerization method, the crosslinking agent is selected from one or a composition of more than two of methyl acrylate, ethylene glycol dimethacrylate, urethane, divinylbenzene and N, N-methylene bisacrylamide, and the mass of the crosslinking agent accounts for 5-30 wt% of the mass of methyl methacrylate MMA monomers.
3. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 2, characterized in that: the adhesive comprises one of poly (butyl acrylate-styrene), poly (N-butyl acrylate-acrylic acid), polyethyl acrylate, poly (N-isopropylacrylamide), poly (ethyl acrylate-styrene), poly (butyl acrylate-methacrylic acid), poly (N-butyl methacrylate), poly (methyl methacrylate-butyl acrylate) or poly (vinyltoluene-t-butylstyrene-methacrylic acid).
4. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 3, characterized in that: the addition amount of the high cross-linked polymethyl methacrylate microspheres per unit square meter is 20-500g, and the addition amount of the adhesive per unit square meter accounts for 1.5-4.5% of the addition amount of the high cross-linked polymethyl methacrylate microspheres per unit square meter.
5. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 4, characterized in that: the surfactant is a nonionic surfactant and comprises one of p-octyl phenoxy polyoxyethylene ether, p-nonyl phenoxy polyoxyethylene ether, polyoxyethylene sorbitan monolaurate or octyl glucoside.
6. The dry analytical reagent according to claim 5 for the detection of γ -glutamyl transpeptidase, wherein: the surfactant is preferably a nonionic surfactant of a chain structure consisting of 8 to 15 oxyethylene or oxypropylene groups linked to one another.
7. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 6, characterized in that: the buffer is a pH buffer with a pH value ranging from 7.0 to 9.0, and the buffer contains tris (hydroxymethyl) aminomethane (tris), phosphate, borate and lysine; one of a sodium salt or a potassium salt of N, N-bis (2-hydroxyethyl) glycine, N-2-hydroxyethylpiperazine-N ' -2-hydroxypropane-3-sulfonic acid, a sodium salt or a potassium salt of N-2-hydroxyethylpiperazine-N ' -3-sulfonic acid, a sodium salt or a potassium salt of N- [ tris (hydroxymethyl) methyl ] -3-aminopropane sulfonic acid and a sodium salt or a potassium salt of N-2-hydroxyethylpiperazine-N ' -2-ethanesulfonic acid, a sodium salt or a potassium salt of 1, 4-piperazine-diethylsulfonic acid (PIPES), and an acid, a base, or a salt in combination with any one of the above compounds.
8. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 7, characterized in that: the stabilizing agent is one or more of polysaccharide or disaccharide; the water-soluble high molecular polymer is one or a mixture of more of gelatin, gelatin derivatives, agarose, dextran, polyvinyl alcohol, polyacrylamide and hydrophilic cellulose derivatives.
9. The dry analytical reagent for the detection of γ -glutamyl transpeptidase according to claim 8, characterized in that: the upper support body (1), the lower support body (5) and the middle support body (6) are all transparent plastic base materials with light transmittance of more than 80% under the wavelength of light of 200nm-900nm, comprise one of polyethylene terephthalate, polycarbonate, polypropylene and polyethylene, and have the thickness of 50-300 mu m.
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| CN202211010894.3A CN115436617A (en) | 2022-08-23 | 2022-08-23 | Dry analytical reagent for gamma-glutamyl transpeptidase detection |
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| CN202211010894.3A CN115436617A (en) | 2022-08-23 | 2022-08-23 | Dry analytical reagent for gamma-glutamyl transpeptidase detection |
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